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7 results on '"MESH: Solubility"'

1. Identification of a Paracrine Signaling Mechanism Linking CD34high Progenitors to the Regulation of Visceral Fat Expansion and Remodeling

Author

Pires, Karla, Buffolo, Márcio, Pires, Karla Maria, Ferhat, Maroua, Ilkun, Olesya, Makaju, Aman, Achenbach, Alan, Bowman, Faith, Atkinson, Donald, Holland, William, Amri, Ez-Zoubir, Chaurasia, Bhagirath, Franklin, Sarah, Boudina, Sihem, Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
MESH: Signal Transduction, 0301 basic medicine, MESH: Rosiglitazone, [SDV]Life Sciences [q-bio], visceral fat, MESH: Bone Morphogenetic Protein 4, Adipose tissue, Inflammation, MESH: Muscle, Smooth, MESH: Stem Cells, macromolecular substances, MESH: Phenotype, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Paracrine signalling, 0302 clinical medicine, Insulin resistance, adipose progenitors, MESH: Mice, Inbred C57BL, Fibrosis, medicine, MESH: Animals, MESH: Paracrine Communication, Progenitor cell, cell sorting, MESH: Intra-Abdominal Fat, lcsh:QH301-705.5, Chemistry, technology, industry, and agriculture, MESH: Antigens, CD34, medicine.disease, fat expansion, MESH: Male, humanities, Cell biology, MESH: Solubility, Transplantation, MESH: Insulin Resistance, 030104 developmental biology, MESH: Adipose Tissue, White, lcsh:Biology (General), MESH: Fibroblasts, Adipogenesis, MESH: Weight Gain, medicine.symptom, MESH: Adipogenesis, 030217 neurology & neurosurgery, transplantation
Abstract

Summary: Accumulation of visceral (VIS) is a predictor of metabolic disorders and insulin resistance. This is due in part to the limited capacity of VIS fat to buffer lipids allowing them to deposit in insulin-sensitive tissues. Mechanisms underlying selective hypertrophic growth and tissue remodeling properties of VIS fat are not well understood. We identified subsets of adipose progenitors (APs) unique to VIS fat with differential Cd34 expression and adipogenic capacity. VIS low (Cd34 low) APs are adipogenic, whereas VIS high (Cd34 high) APs are not. Furthermore, VIS high APs inhibit adipogenic differentiation of SUB and VIS low APs in vitro through the secretion of soluble inhibitory factor(s). The number of VIS high APs increased with adipose tissue expansion, and their abundance in vivo caused hypertrophic growth, fibrosis, inflammation, and metabolic dysfunction. This study unveils the presence of APs unique to VIS fat involved in the paracrine regulation of adipogenesis and tissue remodeling. : Buffolo et al. show that the visceral fat of mice contains subsets of Cd34 positive stromal cells with distinct adipogenic potential. Stromal cells with high Cd34 expression inhibit adipogenic conversion of cells with low Cd34 expression. When implanted in vivo, Cd34 high stromal cells cause visceral adipose tissue remodeling and inflammation and impair insulin sensitivity. Keywords: adipose progenitors, fat expansion, cell sorting, transplantation, visceral fat

Published
2019
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2. Merging in-solution X-ray and neutron scattering data allows fine structural analysis of membrane-protein detergent complexes

Author

Giulia Tamburrino, Frank Gabel, Jochen S. Hub, Olwyn Byron, Tim Rasmussen, Ulrich Zachariae, Miloš T. Ivanović, Arnaud Javelle, Felix M. Strnad, Paul A. Hoskisson, Gaëtan Dias Mirandela, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, Physics, School of Science and Engineering, University of Dundee, Theoretical Physics, Saarland University [Saarbrücken], Institute for Microbiology and Genetics [Göttingen], Georg-August-University [Göttingen], ePlant Science Group, School of Life Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, School of Chemical Sciences and Pharmacy, University of East Anglia [Norwich] (UEA), Division of Physics, School of Engineering, Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Georg-August-University = Georg-August-Universität Göttingen, Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
Materials science, Detergents, Ab initio, Molecular Dynamics Simulation, Neutron scattering, 010402 general chemistry, 01 natural sciences, Chemistry Techniques, Analytical, Turn (biochemistry), 03 medical and health sciences, X-Ray Diffraction, Molecule, MESH: Molecular Dynamics Simulation, 030304 developmental biology, 0303 health sciences, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Small-angle X-ray scattering, MESH: X-Ray Diffraction, X-ray, Membrane Proteins, MESH: Chemistry Techniques, Analytical, MESH: Neutron Diffraction, 0104 chemical sciences, QD450, MESH: Solubility, Neutron Diffraction, Crystallography, Solubility, Membrane protein, Membrane protein complex, MESH: Membrane Proteins, MESH: Detergents
Abstract

International audience; In-solution small-angle X-ray and neutron scattering (SAXS/SANS) have become popular methods to characterize the structure of membrane proteins, solubilized by either detergents or nanodiscs. SANS studies of protein-detergent complexes usually require deuterium-labeled proteins or detergents, which in turn often lead to problems in their expression or purification. Here, we report an approach whose novelty is the combined analysis of SAXS and SANS data from an unlabeled membrane protein complex in solution in two complementary ways. First, an explicit atomic analysis, including both protein and detergent molecules, using the program WAXSiS, which has been adapted to predict SANS data. Second, the use of MONSA which allows one to discriminate between detergent head- and tail-groups in an ab initio approach. Our approach is readily applicable to any detergent-solubilized protein and provides more detailed structural information on protein-detergent complexes from unlabeled samples than SAXS or SANS alone.

Published
2018
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3. Synthesis and characterization of water-soluble macrocyclic peptides stabilizing protein α-turn

Author

Wang, Lei, Coric, Pascale, Zhu, Kexin, Liu, Wang-Qing, Vidal, Michel, Bouaziz, Serge, Broussy, Sylvain, Wang, Wang-Qing, Bouaziz, Serge, Science & Technology Information Institute, Shandong Academy of Agricultural Science, Laboratoire de cristallographie et RMN biologiques (LCRB - UMR 8015), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Pharmacochimie Moléculaire et Cellulaire (PMC - UMR_S 648), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Unité de Pharmacologie Chimique et Génétique (UPCG - UMR_S 640/UMR 8151), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut de Recherche pour le Développement (IRD)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie de coordination (LCC), Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5)-Institut des sciences du Médicament -Toxicologie - Chimie - Environnement (IFR71), Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Protein Conformation, alpha-Helical, Stereochemistry, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Peptide, Chemistry Techniques, Synthetic, Molecular Dynamics Simulation, 010402 general chemistry, 01 natural sciences, Biochemistry, Peptides, Cyclic, Turn (biochemistry), Protein structure, MESH: Protein Stability, Side chain, MESH: Water, MESH: Molecular Dynamics Simulation, MESH: Hydrogen Bonding, Physical and Theoretical Chemistry, Conformational isomerism, MESH: Peptides, Cyclic, chemistry.chemical_classification, MESH: Protein Conformation, alpha-Helical, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], 010405 organic chemistry, Chemistry, Hydrogen bond, MESH: Peptides, Protein Stability, Organic Chemistry, Water, Hydrogen Bonding, 0104 chemical sciences, Amino acid, MESH: Solubility, Solubility, Covalent bond, MESH: Chemistry Techniques, Synthetic, Peptides
Abstract

International audience; Short peptides composed of naturally occurring amino acids are usually unstructured in aqueous media. The installation of covalent constraints within their side chains or backbones, resulting in the formation of macrocyclic peptides, is an appealing approach to stabilize them in defined secondary structures. Therefore, with the objective to stabilize α-turn conformation, we designed, synthesized and characterized constrained 13-membered macrocyclic peptides. Their design was inspired by previous work using the replacement of a hydrogen bond by a covalent bond, for the stabilization of α-helical secondary structures. Their synthesis employed our recently published solid-phase method based on Fukuyama-Mitsunobu alkylation reactions. We report herein an optimized synthesis leading to three water-soluble 13-membered macrocyclic peptides 10a-c, including respectively two, one and zero glycine residues. They were characterized by CD and NMR, which indicated the presence of equilibrating conformers. The detailed conformational analysis was based on extensive NMR and molecular dynamics studies. We found that the peptide without glycine residues 10c was mostly present as slowly interconverting conformers whereas the peptide with two glycine residues 10a was mostly present as rapidly interconverting conformers. We did not find a good match between the conformers of 10a and α-turns occurring in proteins, due to the high flexibility of the glycine backbone. Interestingly, we found that the major conformer of 10c accurately matched the "non-classical" or "tight" α-turn of type II-αLS, with a RMSD value of 0.42 Å for heavy atoms constituting the macrocycle. This is, to the best of our knowledge, the first molecule reported to mimic this type of α-turn found in proteins.

Published
2017
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4. Toxoplasma gondii: Biochemical and biophysical characterization of recombinant soluble dense granule proteins GRA2 and GRA6

Author

Guy Schoehn, Jean Gagnon, Laetitia Travier, Grégory Effantin, Corinne Mercier, Graciane Petre, Amina Bittame, Winfried Weissenhorn, Pauline Ruffiot, Marie-France Cesbron-Delauw, Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Thomas, Frank, Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)

Subjects
Protein Folding, Circular dichroism, Light, [SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], MESH: Protein Folding, Population, Protozoan Proteins, Biophysics, MESH: Protein Structure, Secondary, Antigens, Protozoan, Biology, Biochemistry, Protein Structure, Secondary, MESH: Circular Dichroism, law.invention, MESH: Recombinant Proteins, Microscopy, Electron, Transmission, law, parasitic diseases, Scattering, Radiation, MESH: Scattering, Radiation, education, MESH: Protozoan Proteins, Molecular Biology, Protein secondary structure, education.field_of_study, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Circular Dichroism, Intracellular parasite, Toxoplasma gondii, Cell Biology, biology.organism_classification, Recombinant Proteins, MESH: Light, Cell biology, MESH: Solubility, Solubility, MESH: Microscopy, Electron, Transmission, Recombinant DNA, Dense granule, Biogenesis, MESH: Antigens, Protozoan
Abstract

International audience; The most prominent structural feature of the parasitophorous vacuole (PV) in which the intracellular parasite Toxoplasma gondii proliferates is a membranous nanotubular network (MNN), which interconnects the parasites and the PV membrane. The MNN function remains unclear. The GRA2 and GRA6 proteins secreted from the parasite dense granules into the PV have been implicated in the MNN biogenesis. Amphipathic alpha-helices (AAHs) predicted in GRA2 and an alpha-helical hydrophobic domain predicted in GRA6 have been proposed to be responsible for their membrane association, thereby potentially molding the MMN in its structure. Here we report an analysis of the recombinant proteins (expressed in detergent-free conditions) by circular dichroism, which showed that full length GRA2 displays an alpha-helical secondary structure while recombinant GRA6 and GRA2 truncated of its AAHs are mainly random coiled. Dynamic light scattering and transmission electron microscopy showed that recombinant GRA6 and truncated GRA2 constitute a homogenous population of small particles (6-8 nm in diameter) while recombinant GRA2 corresponds to 2 populations of particles (∼8-15 nm and up to 40 nm in diameter, respectively). The unusual properties of GRA2 due to its AAHs are discussed.

Published
2015
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5. Effects of urban coarse particles inhalation on oxidative and inflammatory parameters in the mouse lung and colon

Author

Laurent Y. Alleman, Adil Ouali Alami, Corinne Gower-Rousseau, Madjid Djouina, Pierre Desreumaux, Mathilde Body-Malapel, Esperanza Perdrix, Cécile Vignal, Muriel Pichavant, Florian Dingreville, Christophe Waxin, Body-Malapel, Mathilde, Lille Inflammation Research International Center - U 995 (LIRIC), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Département Sciences de l'Atmosphère et Génie de l'Environnement, École des Mines de Douai (Mines Douai EMD), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Service de Gastroenterologie [CHRU Lille], Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), This work was supported by the Hauts-de-France Region and the Ministère de l’Enseignement Supérieur et de la Recherche (CPER Climibio), the European Fund for Regional Economic Development, and Digestscience (European Research Foundation on Intestinal Diseases and Nutrition)., The authors thank Bruno Mallet., Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Inserm, Université de Lille, CHU Lille, Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 8204, Lille Inflammation Research International Center - U 995 [LIRIC], and Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]

Subjects
0301 basic medicine, Male, Coarse PM, MESH: Air Pollutants, Health, Toxicology and Mutagenesis, medicine.medical_treatment, MESH: Solvents, 010501 environmental sciences, Toxicology, medicine.disease_cause, 01 natural sciences, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Antioxidants, 11. Sustainability, Gut-lung axis, N-acetyl-L-cysteine, MESH: Animals, [SDV.IMM.ALL]Life Sciences [q-bio]/Immunology/Allergology, Lung, Air Pollutants, Inhalation Exposure, MESH: Cytokines, MESH: Oxidative Stress, Inhalation, Mesh:Air Pollutants/toxicity, Mesh:Solubility, Mesh:Inflammation Mediators/immunology, Mesh:Mice, Mesh:Inbred C57BL, Mesh:Colon/immunology, Mesh:Colon/metabolism, Mesh:Acetylcysteine/pharmacology, Mesh:Animals, Mesh:Cytokines/metabolism, Mesh:Oxidative Stress/drug effects, Mesh:Colon/drug effects, Mesh:Water/chemistry, Mesh:Lung/drug effects, Mesh:Lung/metabolism, Mesh:Cytokines/immunology, Mesh:Air Pollutants/chemistry, Mesh:Antioxidants/pharmacology, Mesh:Male, Mesh:Inflammation Mediators/metabolism, Mesh:Particulate Matter/chemistry, Mesh:Inhalation Exposure/adverse effects, Mesh:Lung/immunology, Mesh:Particulate Matter/toxicity, Mesh:Particle Size, Mesh:Solvents/chemistry, Inflammation, Particulate matter, Oxidative stress, General Medicine, 3. Good health, medicine.anatomical_structure, Cytokine, MESH: Particulate Matter, Bronchitis, Cytokines, MESH: Inhalation Exposure, medicine.symptom, Inflammation Mediators, [SDV.IMM.ALL] Life Sciences [q-bio]/Immunology/Allergology, Colon, MESH: Inflammation Mediators, lcsh:Industrial hygiene. Industrial welfare, 03 medical and health sciences, Immune system, MESH: Acetylcysteine, lcsh:RA1190-1270, MESH: Mice, Inbred C57BL, medicine, MESH: Water, Animals, [SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health, MESH: Lung, MESH: Particle Size, Particle Size, MESH: Colon, 0105 earth and related environmental sciences, Asthma, lcsh:Toxicology. Poisons, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, business.industry, Research, MESH: Antioxidants, Water, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, medicine.disease, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, MESH: Male, Acetylcysteine, Mice, Inbred C57BL, MESH: Solubility, 030104 developmental biology, Solubility, 13. Climate action, Immunology, Solvents, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, business, lcsh:HD7260-7780.8
Abstract

Background Air pollution is a recognized aggravating factor for pulmonary diseases and has notably deleterious effects on asthma, bronchitis and pneumonia. Recent studies suggest that air pollution may also cause adverse effects in the gastrointestinal tract. Accumulating experimental evidence shows that immune responses in the pulmonary and intestinal mucosae are closely interrelated, and that gut-lung crosstalk controls pathophysiological processes such as responses to cigarette smoke and influenza virus infection. Our first aim was to collect urban coarse particulate matter (PM) and to characterize them for elemental content, gastric bioaccessibility, and oxidative potential; our second aim was to determine the short-term effects of urban coarse PM inhalation on pulmonary and colonic mucosae in mice, and to test the hypothesis that the well-known antioxidant N-acetyl-L-cysteine (NAC) reverses the effects of PM inhalation. Results The collected PM had classical features of urban particles and possessed oxidative potential partly attributable to their metal fraction. Bioaccessibility study confirmed the high solubility of some metals at the gastric level. Male mice were exposed to urban coarse PM in a ventilated inhalation chamber for 15 days at a concentration relevant to episodic elevation peak of air pollution. Coarse PM inhalation induced systemic oxidative stress, recruited immune cells to the lung, and increased cytokine levels in the lung and colon. Concomitant oral administration of NAC reversed all the observed effects relative to the inhalation of coarse PM. Conclusions Coarse PM-induced low-grade inflammation in the lung and colon is mediated by oxidative stress and deserves more investigation as potentiating factor for inflammatory diseases. Electronic supplementary material The online version of this article (10.1186/s12989-017-0227-z) contains supplementary material, which is available to authorized users.

Published
2017
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6. Soluble Siglec-5 associates to PSGL-1 and displays anti-inflammatory activity

Author

Pepin, Marion, Mezouar, Soraya, Pegon, Julie, Muczynski, Vincent, Adam, Frédéric, Bianchini, Elsa P, Bazaa, Amine, Proulle, Valerie, Rupin, Alain, Paysant, Jerome, Panicot-Dubois, Laurence, Christophe, Olivier D., Dubois, Christophe, Lenting, Peter J, Denis, Cécile V, Hémostase, Inflammation, Thrombose (HITH - U1176 Inserm - CHU Bicêtre), Université Paris-Sud - Paris 11 (UP11)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre)-Institut National de la Santé et de la Recherche Médicale (INSERM), Vascular research center of Marseille (VRCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Aix Marseille Université (AMU), Service d'hématologie, immunologie biologiques et cytogénétique, Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Bicêtre, Institut de Recherches Internationales Servier [Suresnes] (IRIS), The study was financially supported by a PhD-research grant from Institut Servier & Association Nationale de la Recherche Technique (Contrat CIFRE) to JPe, and an Inserm-Poste d’Accueil-grant (# ASC13101LSA) to MP., Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
MESH: Anti-Inflammatory Agents/pharmacology, E-SELECTIN LIGAND-1, [SDV]Life Sciences [q-bio], Anti-Inflammatory Agents, MESH: Membrane Glycoproteins/genetics, MESH: Antigens, Differentiation, Myelomonocytic/metabolism, MESH: Lectins/metabolism, MESH: Antigens, CD/pharmacology, MESH: Leukocytes, Mononuclear/drug effects, Lectins, MESH: Animals, MYELOID CELLS, MESH: E-Selectin/metabolism, NEUTROPHILS, IN-VIVO, MESH: Inflammation/drug therapy, Membrane Glycoproteins, MESH: Inflammation/chemically induced, MESH: Inflammation/pathology, MESH: Membrane Glycoproteins/metabolism, MESH: Leukocyte Rolling/physiology, P-Selectin, Female, E-Selectin, MESH: Leukocytes, Mononuclear/metabolism, CD33-RELATED SIGLECS, VON-WILLEBRAND-FACTOR, MESH: Lectins/pharmacology, Antigens, Differentiation, Myelomonocytic, MESH: Antigens, Differentiation, Myelomonocytic/pharmacology, MESH: Tumor Necrosis Factor-alpha/toxicity, GLYCOPROTEIN LIGAND, MESH: Antigens, CD/metabolism, Article, MESH: Mice, Inbred C57BL, Antigens, CD, Animals, Humans, Leukocyte Rolling, Protein Interaction Domains and Motifs, MESH: Antigens, Differentiation, Myelomonocytic/genetics, SIALIC-ACID, Inflammation, MESH: Protein Interaction Domains and Motifs, MESH: Humans, Tumor Necrosis Factor-alpha, MESH: Lectins/genetics, MESH: Solubility, Mice, Inbred C57BL, Disease Models, Animal, Solubility, Leukocytes, Mononuclear, MESH: Antigens, CD/genetics, IMMUNE-SYSTEM, MESH: Disease Models, Animal, MESH: Female, MESH: P-Selectin/metabolism
Abstract

International audience; Interactions between endothelial selectins and the leukocyte counter-receptor PSGL1 mediates leukocyte recruitment to inflammation sites. PSGL1 is highly sialylated, making it a potential ligand for Siglec-5, a leukocyte-receptor that recognizes sialic acid structures. Binding assays using soluble Siglec-5 variants (sSiglec-5/C4BP and sSiglec-5/Fc) revealed a dose- and calcium-dependent binding to PSGL1. Pre-treatment of PSGL1 with sialidase reduced Siglec-5 binding by 79 ± 4%. In confocal immune-fluorescence assays, we observed that 50% of Peripheral Blood Mononuclear Cells (PBMCs) simultaneously express PSGL1 and Siglec-5. Duolink-proximity ligation analysis demonstrated that PSGL1 and Siglec-5 are in close proximity (

Published
2016
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7. Highly Fluorescent and Water-Soluble Diketopyrrolopyrrole Dyes for Bioconjugation

Author

Elodie Heyer, Pauline Lory, Anthony Romieu, Massimo Guardigli, Aldo Roda, Raymond Ziessel, Jérôme Leprince, Mathieu Moreau, Heyer, Elodie, Lory, Pauline, Leprince, Jérôme, Moreau, Mathieu, Romieu, Anthony, Guardigli, Massimo, Roda, Aldo, Ziessel, Raymond, Institut de chimie et procédés pour l'énergie, l'environnement et la santé (ICPEES), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Différenciation et communication neuronale et neuroendocrine (DC2N), Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Matériaux, Surfaces et Procédés pour la Catalyse (LMSPC), and Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE)

Subjects
Carboxylic acid, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Fluorescent Dye, Quantum yield, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, Photochemistry, Pyrrole, 01 natural sciences, Fluorescence spectroscopy, Fluorescence, Catalysis, chemistry.chemical_compound, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Fluorescence microscope, MESH: Water, Organic chemistry, Pyrroles, Fluorescein, Bovine serum albumin, Fluorescent Dyes, chemistry.chemical_classification, Bioconjugation, biology, 010405 organic chemistry, Synthetic method, Protein, Chemistry (all), Dyes/pigment, Water, General Chemistry, General Medicine, MESH: Fluorescent Dyes, proteins, 0104 chemical sciences, imaging agents, MESH: Solubility, chemistry, Solubility, biology.protein, synthetic methods, MESH: Pyrroles, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, dyes/pigments, Imaging agent
Abstract

International audience; The preparation of highly water-soluble and strongly fluorescent diketopyrrolopyrrole (DPP) dyes using an unusual taurine-like sulfonated linker has been achieved. Exchanging a phenyl for a thienyl substituent shifts the emission wavelength to near λ=600 nm. The free carboxylic acid group present in these new derivatives was readily activated and the dyes were subsequently covalently linked to a model protein (bovine serum albumin; BSA). The bioconjugates were characterized by electronic absorption, fluorescence spectroscopy and MALDI-TOF mass spectrometry, thus enabling precise determination of the labeling density (ratio DPP/BSA about 3 to 8). Outstanding values of fluorescence quantum yield (30% to 59%) for these bioconjugates are obtained. The photostability of these DPP dyes is considerably greater than that of fluorescein under the same irradiation conditions. Remarkably low detection limits between 80 and 300 molecules/μm(2) were found for the BSA bioconjugates by fluorescence imaging with a epifluorescence microscope.

Published
2015
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