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Purpose: Although running traditionally relies on critical speed (CS) as an indicator of critical intensity, portable inertial measurement units offer a potential solution for estimating running mechanical power to assess critical power (CP) in runners. The purpose of this study was to determine whether CS and CP differ when assessed using the Stryd device, a portable inertial measurement unit, and if 2 running bouts are sufficient to determine CS and CP. Methods: On an outdoor running track, 10 trained runners ( V ˙ O 2 max , 59.0 [4.2] mL·kg−1·min−1) performed 3 running time trials (TT) between 1200 and 4400 m on separate days. CS and CP were derived from 2-parameter hyperbolic speed–time and power–time models, respectively, using 2 (CS2TT and CP2TT) and 3 (CS3TT and CP3TT) TTs. Subsequently, runners performed constant-intensity running for 800 m at their calculated CS3TT and CP3TT. Results: Running at the calculated CS3TT speed (3.88 [0.44] m·s−1) elicited an average Stryd running power (271 [28] W) not different from the calculated CP3TT (270 [28]; P =.940; d = 0.02), with excellent agreement between the 2 values (intraclass correlation coefficient =.980). The CS2TT (3.97 [0.42] m·s−1) was not higher than CS3TT (3.89 [0.44] m·s−1; P =.178; d = 0.46); however, CP2TT (278 [29] W) was greater than CP3TT (P =.041; d = 0.75). Conclusion: The running intensities at CS and CP were similar, supporting the use of running power (Stryd) as a metric of aerobic fitness and exercise prescription, and 2 trials provided a reasonable, albeit higher, estimate of CS and CP. [ABSTRACT FROM AUTHOR]

Blood properties influence aerobic exercise performance. While vascular volumes and hemoglobin mass (Hbmass) are elevated in trained individuals, evidence of sex differences in vascular volumes is equivocal due to inadequate matching of aerobic fitness between males and females. This cross-sectional study aimed to compare hematological values normalized to body mass (BM) and fat-free mass (FFM) between males (n = 45) and females (n = 34) matched for aerobic fitness (V̇O2max) normalized to FFM (mL∙kg FFM−1∙min−1). Data included body composition measured by dual-energy X-ray absorptiometry (DXA), V̇O2max from an incremental test, and hematological values derived from a CO rebreathe test. Fat mass was unrelated to blood volume (BV; R2 = 0.02, P = 0.26) and Hbmass (R2 = 0.03, P = 0.16), while FFM was the strongest predictor of both (R2 = 0.75 and R2 = 0.83, respectively, P < 0.001). Females exhibited higher FFM-normalized BV (+4%, P < 0.05) and plasma volume (PV) (+14%, P < 0.001) and lower red blood cell volume (RBCV) (−8%, P < 0.001) and Hbmass (−8%, P < 0.001) compared to males. Positive correlations between aerobic fitness and relative Hbmass and BV were observed in both sexes when normalized to BM and FFM (0.48 < r < 0.71; P < 0.003). Stepwise multiple regression models, including FFM, V̇O2max, height, and [Hb], provided accurate predictions of Hbmass (R2 = 0.91) and BV (R2 = 0.85). Overall, sex differences persist in relative Hbmass, BV, PV, and RBCV after matching of aerobic fitness, though relative BV and PV were greater in females. These findings suggest sex-specific strategies in oxygen delivery and/or extraction, and they underscore the importance of carefully selecting normalization practices when assessing sex-based differences in hematological variables. [ABSTRACT FROM AUTHOR]

Few studies have explored the kinetics of performance and perceived fatigability during high‐intensity interval training, despite its popularity. We aimed to characterize the kinetics of fatigability and recovery during an 8 × 4‐min HIIT protocol, hypothesizing that most muscle function impairment would occur during the initial four intervals. Fifteen healthy males and females (mean ± standard deviation; age = 26 ± 5 years, V̇O2max = 46.8 ± 6.1 mL·kg−1·min−1) completed eight, 4‐min intervals at 105% of critical power with 3 min of rest. Maximal voluntary knee extension contractions (MVCs) coupled with electrical nerve stimulation were performed at baseline and after the first, fourth, and eighth intervals. MVC, potentiated twitch force (Pt), and Db10:100 ratio all declined throughout HIIT (p < 0.05). MVC sharply declined after interval 1 (−15 ± 9% relative to baseline; p < 0.05) and had only further declined after interval 8 (−26 ± 11%; p < 0.05), but not interval 4 (−19 ± 13%; p > 0.05). Pt and Db10:100 also sharply declined after interval 1 (Pt: −18 ± 13%, Db10:100: −14 ± 20%; p < 0.05) and further declined after interval 4 (Pt: −35 ± 19%, Db10:100: −30 ± 20%; p < 0.05) but not interval 8 (Pt: −41 ± 19%; Db10:100: −32 ± 18%; p > 0.05). Voluntary activation did not significantly change across the HIIT protocol (p > 0.05). Evoked force recovery was significantly blunted as more intervals were completed: after interval 1, Pt recovered by 7 ± 11% compared to −6 ± 7% recovery after interval 8 (p < 0.05). Ratings of perceived effort, fatigue, and leg pain rose throughout the session (p < 0.05 for each) and were greater (effort and fatigue) for females (p < 0.05). Otherwise, males and females exhibited similar performance fatigability kinetics, with contractile function declines blunted in response to additional intervals. [ABSTRACT FROM AUTHOR]

Oral iron supplementation is the first-line treatment for addressing iron deficiency, a concern particularly relevant to women who are susceptible to sub-optimal iron levels. Nevertheless, the impact of iron supplementation on the gut microbiota of middle-aged women remains unclear. To investigate the association between iron supplementation and the gut microbiota, healthy females aged 40–65 years (n = 56, BMI = 23 ± 2.6 kg/m2) were retrospectively analyzed from the Alberta's Tomorrow Project. Fecal samples along with various lifestyle, diet, and health questionnaires were obtained. The gut microbiota was assessed by 16S rRNA sequencing. Individuals were matched by age and BMI and classified as either taking no iron supplement, a low-dose iron supplement (6–10 mg iron/day), or high-dose iron (>100 mg/day). Compositional and functional analyses of microbiome data in relation to iron supplementation were investigated using various bioinformatics tools. Results revealed that iron supplementation had a dose-dependent effect on microbial communities. Elevated iron intake (>100 mg) was associated with an augmentation of Proteobacteria and a reduction in various taxa, including Akkermansia, Butyricicoccus, Verrucomicrobia, Ruminococcus, Alistipes, and Faecalibacterium. Metagenomic prediction further suggested the upregulation of iron acquisition and siderophore biosynthesis following high iron intake. In conclusion, adequate iron levels are essential for the overall health and wellbeing of women through their various life stages. Our findings offer insights into the complex relationships between iron supplementation and the gut microbiota in middle-aged women and underscore the significance of iron dosage in maintaining optimal gut health. [ABSTRACT FROM AUTHOR]

To further refine the near-infrared spectroscopy (NIRS)-derived measure of skeletal muscle oxidative capacity in humans, we sought to determine whether the exercise stimulus intensity affected the τ value and/or influenced the magnitude of correlations with in vitro measures of mitochondrial content and in vivo indices of exercise performance. Males (n = 12) and females (n = 12), matched for maximal aerobic fitness per fat-free mass, completed NIRS-derived skeletal muscle oxidative capacity tests for the vastus lateralis following repeated contractions at 40% (`40) and 100% (τ100) of maximum voluntary contraction, underwent a skeletal muscle biopsy of the same muscle, and performed multiple intermittent isometric knee extension tests to task failure to establish critical torque (CT). The value of τ100 (34.4 ± 7.0 s) was greater than τ40 (24.2 ± 6.9 s, P < 0.001), but the values were correlated (r = 0.688; P < 0.001). The values of τ40 (r = -0.692, P < 0.001) and τ100 (r = -0.488, P = 0.016) correlated with myosin heavy chain I percentage and several markers of mitochondrial content, including COX II protein content in whole muscle (s40: r = -0.547, P = 0.006; τ100: r = -0.466, P = 0.022), type I pooled fibers (s40: r = -0.547, P = 0.006; τ100: r = -0.547, P = 0.006), and type II pooled fibers (s40: r = -0.516, P = 0.009; τ100: r = -0.635, P = 0.001). The value of τ40 (r = -0.702, P < 0.001), but not τ100 (r = -0.378, P = 0.083) correlated with critical torque (CT); however, neither value correlated with W0 (τ40: r = 0.071, P = 0.753; τ100: r = 0.054, P = 0.812). Overall, the NIRS method of assessing skeletal muscle oxidative capacity is sensitive to the intensity of skeletal muscle contraction but maintains relationships to whole body fitness, isolated limb critical intensity, and mitochondrial content regardless of intensity. [ABSTRACT FROM AUTHOR]

We tested the hypothesis that presleep consumption of α-lactalbumin (LA), a fraction of whey with a high abundance of tryptophan, would improve indices of sleep quality and time-trial (TT) performance in cyclists relative to an isonitrogenous collagen peptide (CP) supplement lacking tryptophan. Using randomized, double-blind, crossover designs, cyclists consumed either 40 g of LA or CP 2 hr prior to sleep. In Study 1, six elite male endurance track cyclists (age 23 ± 6 years, ...O2peak 70.2 ± 4.4 ml⋅kg-1⋅min-1) consumed a supplement for three consecutive evenings before each 4-km TT on a velodrome track, whereas in Study 2, six well-trained cyclists (one female; age 24 ± 5 years, ...O2peak 66.9 ± 8.3 ml⋅kg-1⋅min-1) consumed a supplement the evening before each 4-km TT on a stationary cycle ergometer. Indices of sleep quality were assessed with wrist-based actigraphy. There were no differences between the CP and LA supplements in terms of total time in bed, total sleep time, or sleep efficiency in Study 1 (LA: 568 ± 71 min, 503 ± 67 min, 88.3% ± 3.4%; CP: 546 ± 30 min, 479 ± 35 min, 87.8% ± 3.1%; p = .41, p = .32, p = .74, respectively) or Study 2 (LA: 519 ± 90 min, 450 ± 78 min, 87.2% ± 7.6%; CP: 536 ± 62 min, 467 ± 57 min, 87.3% ± 6.4%; p = .43, p = .44, p = .97, respectively). Similarly, time to complete the 4-km TT was unaffected by supplementation in Study 1 (LA: 274.9 ± 7.6 s; CP: 275.5 ± 7.2 s; p = .62) and Study 2 (LA: 344.3 ± 22.3 s; CP: 343.3 ± 23.0 s; p = .50). Thus, relative to CP, consuming LA 2 hr prior to sleep over 1-3 days did not improve actigraphy-based indices of sleep quality or 4-km TT performance in cyclists. [ABSTRACT FROM AUTHOR]

Purpose: The mean power output (MPO) from a 60-min time trial (TT)—also known as functional threshold power, or FTP—is a standard measure of cycling performance; however, shorter performance tests are desirable to reduce the burden of performance testing. The authors sought to determine the reliability of 4- and 20-min TTs and the extent to which these short TTs were associated with 60-min MPO. Methods: Trained male cyclists (n = 8; age = 25 [5] y; V O 2 max = 71 [5] mL/kg/min) performed two 4-min TTs, two 20-min TTs, and one 60-min TT. Critical power (CP) was estimated from 4- and 20-min TTs. The typical error of the mean (TEM) and intraclass correlation coefficient (ICC) were calculated to assess reliability, and R2 values were calculated to assess relationships with 60-min MPO. Results: Pairs of 4-min TTs (mean: 417 [SD: 45] W vs 412 [49] W, P =.25; TEM = 8.1 W; ICC =.98), 20-min TTs (342 [36] W vs 344 [33] W, P =.41; TEM = 4.6 W; ICC =.99), and CP estimates (323 [35] W vs 328 [32] W, P =.25; TEM = 6.5; ICC =.98) were reliable. The 4-min MPO (R2 =.95), 20-min MPO (R2 =.92), estimated CP (R2 =.82), and combination of the 4- and 20-min MPO (adjusted R2 =.98) were strongly associated with the 60-min MPO (309 [26] W). Conclusion: The 4- and 20-min TTs appear useful for assessing performance in trained, if not elite, cyclists. [ABSTRACT FROM AUTHOR]