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Summary This experiment was conducted to assess a protocol combining 20% excess dietary methionine above the modern breeder pullet's requirement and AviPro MGF vaccine in controlling Mycoplasma gallispeticum (MG) infection. In a complete randomized design, a total of 276 6-wk-old breeder pullets, of the Ross 308 strain were allocated into 4 treatments, each with 3 floor pen replicates of 23 birds/pen: 1) nonvaccinated with adequate methionine (NVAM), 2) nonvaccinated with 20% excess methionine (NVEM), 3) Vaccinated with adequate methionine (VAM), 4) Vaccinated with 20% excess methionine (VEM). Vaccination with AviPro MGF at 6 wk of age was performed via drinking water. Results indicated that the rate of depictable growth and tracheal colonization of MGF vaccine strain is 6 wk postvaccination. Sera Elisa titers showed that an 8-wk-postvaccination period was required for the vaccine to stimulate the humoral immunity response against MG. The 20% excess methionine numerically increased the IgG titer against MG in comparison to other groups, yet this increase was not enough to generate protective titer count. Furthermore, the addition of 20% excess methionine above the recommended levels hindered the tracheal colonization rate and log10 values of AviPro MGF vaccine colony forming units from 5.9/mL of tracheal swab suspension in VAM group to 0.82/mL of tracheal swab suspension in VEM group (P ˂ 0.05). In conclusion, the 20% excess methionine above the modern requirements reduced the tracheal colonization of F-strain MG. Further research is needed to confirm the ability of excess dietary methionine to enhance the adaptive and acquired immunity of broiler breeder pullets.

The present work assessed the effect of supplementation of 0.8% dietary Arbocel® RC Fine, a readily available commercial lignocellulose, to poultry feed. In a complete randomized design using 36 individually caged mature dubbed Hy-Line roosters (aged 55 weeks) grouped in 4 treatments with 9 birds per treatment, a digestibility trial was performed to determine apparent and true metabolizable energy values along with digestibility coefficients of protein and amino acid in Arbocel® containing diets. Results showed that 0.8% Arbocel® supplemented diets improved protein digestibility by 6% (P

Egg is considered a nutritionally complete food and an excellent source of protein. However, storing eggs for a prolonged period of time under uncontrolled temperature results in egg quality deterioration. The objective of this study is to determine the effect of storage conditions (time & temperature) on the egg's internal and external quality parameters as well as the microbiological load of eggs. For that purpose, a total of 2160 (white, brown vaccinated and brown non-vaccinated for salmonella) eggs were collected from Lebanese egg farmers in Bekaa valley and stored at 7 °C, 18 °C, 24 °C and 33 °C/20 °C (cyclic) for 2, 4 and 6 weeks. At each time point and temperature setting, 30 eggs were analysed for external and internal quality traits as well microbiological testing. Results showed that brown eggs had significantly higher weight (P

Improving water use efficiency in the agricultural sector, particularly in arid regions, is becoming increasingly important in response to increasing global population and concomitant demand for food, water, and land. The authors performed experiments to determine whether the use of super-absorbent polymers, also known as hydrogels, can increase plant survival, plant growth, and plant water use efficiency. The effect of hydrogel application proportions and application methods on plant survival and growth in clay and sandy clay loam (SCL) soils was investigated. Two pot experiments using Zea mays and Pinus pinea as model plants were performed. The hydrogel was applied at concentrations of 0, 1, 2, 3 and 4 g kg–1 soil in one of two methods: banding or mixing. Hydrogel effects on water retention in the two soil types were recorded. The results suggest that hydrogel banding at 0.4% application in SCL improves corn's fresh and dry above-ground biomass by 25%, and prolongs survival time of pine seedlings by 90%. Evapotranspiration was greater in soils banded with hydrogel at 0.4% and water use efficiency increased by 10–13% in both soils. Additionally, hydrogel increased water retention of SCL by up to 33% at 100 kPa, but had negligible effects when applied in clay soils. Accordingly, the efficacy of hydrogels in agriculture water conservation depends on using appropriate application methods and quantities.