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Objetivo: evaluar efecto genotóxico y mutagénico en linfocitos humanos expuestos a nanotubos de carbono (NTC) prístinos y dopados con Nitrógeno. Métodos: linfocitos humanos fueron expuestos a NTC dopados y prístinos (0.08, 0.09, 0.1mg/mL), se evaluó alteraciones cromosómicas e intercambio de cromátidas hermanas (ICH). Resultados: se presentaron rupturas cromatídicas y cromosómicas en linfocitos expuestos a 0.1mg/mL y 0.08mg/mL de NTC prístinos. NTC dopados, indujeron cromosomas dicéntricos y anillos cromosómicos. Se presentó diferencia significativa en el porcentaje de ICH de células tratadas con NTC dopados versus control negativo y NTC prístinos (p

Arthrogenic muscle inhibition (AMI) refers to muscular alterations that are generated, producing biomechanical motor control and movement problems, leading to deficiencies in strength and atrophy. Currently, there exist methods that involve virtual reality (VR) and have been well perceived by physiotherapists. The present research measured the potential benefits in terms of therapeutic adherence and speed of recovery, through a comparative analysis in a healthcare provider institution, in Medellín, Colombia, with and without the aid of VR. For this purpose, dynamometry, and surface electromyography (sEMG) signal acquisition tools were used. The treatment involved neuromodulation, ranges of motion and mobility work, strengthening and reintegration into movement, complemented with TENS, NMENS and therapeutic exercise, where the patient was expected to receive a satisfactory and faster adherence and recovery. A group of 15 people with AMI who include at least 15 min of VR per session in their treatment were compared with another group who received only the base treatment, i.e., the control group. Analyzing the variables individually, it is possible to affirm that VR, as a complement, statistically significantly improved the therapeutic adherence in 33.3% for CG and 37.5% for IG. Additionally, it increased strength with both legs, the symmetry between them, and decreased the level of pain and stiffness that is related to mobility.

Nano-engineered materials such as carbon nanotubes (CNTs) have gained attention due to their many characteristics which make them the ideal material for applications in biomedical areas. The potential use of CNTs in medical applications is promising but their interaction with the human body and the effects of those interactions are still uncertain. The results of such interactions often determines the clinical success of nano-engineered materials such as CNTs in the medical field; therefore, the aim of this study was to measure the biocompatibility of two types of CNTs with human umbilical artery endothelial cells (HUAEC) and human hepatoma cells (HepG2). The genotoxic and mutagenic activity of pristine and N-doped CNTs was evaluated by trypan blue exclusion test, flow cytometry, chromosomal aberrations, cell cycle dynamics by mitotic inhibition and by flow cytometry as well as single cell gel electrophoresis (SCGE). Data was analyzed by both one-way and two-way analysis of variance (ANOVA) when appropriate, Student's t, Kruskall–Wallis and the chi-squared test (X2) based on data characteristics. Ruptures in the cell's DNA were evidenced by the SCGE on HUAEC and HepG2 cells when exposed to sub-lethal concentrations of both types of CNTs. Results obtained for the cell viability, the cell cycle dynamics and the chromosomal aberrations were not significantly different when compared to the negative control. These findings suggest an increased risk of fixed mutations since the cells continue through the cell cycle although their DNA is compromised.