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Your search keyword '"Lu, Chengping"' showing total 319 results
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319 results on '"Lu, Chengping"'

36. Comparative transcriptome combined with morphophysiological analyses revealed the molecular mechanism underlying Tetrahymena thermophilapredation-induced antiphage defense in Aeromonas hydrophila

Author

Dong, Yuhao, Liu, Jin, Nie, Meng, Zhao, Dan, Huang, Hao, Geng, Jinzhu, Wan, Xihe, Lu, Chengping, and Liu, Yongjie

Abstract

ABSTRACTProtozoan predation has been demonstrated to be a strong driving force for bacterial defence strategies in the environment. Our previous study demonstrated that Aeromonas hydrophilaNJ-35, which evolved small-colony variants (SCVs), displayed various adaptive traits in response to Tetrahymena thermophilapredation, such as enhanced phage resistance. However, the evolutionary mechanisms are largely unknown. In this study, we performed a genome- and transcriptome-wide analysis of the SCV1, representing one strain of the SCVs, for identification of the genes of mutation and altered expression underlying this phage resistance phenotype. Our study demonstrated that phage resistance caused by T. thermophilapredation was due to the downregulation of a flagellar biosynthesis regulator, flhF, in SCV1. Interestingly, we confirmed that phage resistance in SCV1 was not straightforwardly attributable to the absence of flagella but to FlhF-mediated secretion of extracellular protein that hinders phage adsorption. This finding improves our understanding of the mechanisms by which A. hydrophilalowers the susceptibility to phage infection under predation pressure, and highlights an important contribution of bacterium–protozoan interactions in driving the adaptive evolution of pathogens in complex environments.

Published
2022
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39. The Novel Streptococcal Transcriptional Regulator XtgS Negatively Regulates Bacterial Virulence and Directly Represses PseP Transcription

Author

Liu, Guangjin, primary, Gao, Tingting, additional, Zhong, Xiaojun, additional, Ma, Jiale, additional, Zhang, Yumin, additional, Zhang, Shiyu, additional, Wu, Zongfu, additional, Pan, Zihao, additional, Zhu, Yinchu, additional, Yao, Huochun, additional, Liu, Yongjie, additional, and Lu, Chengping, additional

Published
2020
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44. Virulent and Vaccine Strains of Streptococcus equi ssp. zooepidemicus Have Different Influences on Phagocytosis and Cytokine Secretion of Macrophages

Author

Peng Jie, Lu Chengping, Hua Chengwei, Lin Huixing, Fan Hong-jie, Zhang Hui, and Ma Zhe

Subjects
0301 basic medicine, Streptococcus equi, Proteome, Swine, Phagocytosis, 030106 microbiology, Virulence, Biology, Biochemistry, Cell Line, Microbiology, Mice, 03 medical and health sciences, Species Specificity, Tandem Mass Spectrometry, Immunity, Streptococcal Infections, Stable isotope labeling by amino acids in cell culture, Animals, Swine Diseases, Attenuated vaccine, Macrophages, Streptococcal Vaccines, Molecular Sequence Annotation, General Chemistry, Blot, Gene Ontology, 030104 developmental biology, Gene Expression Regulation, Isotope Labeling, Host-Pathogen Interactions, Cytokines, Cytokine secretion, Chromatography, Liquid
Abstract

Swine streptococcosis is a significant threat to the Chinese pig industry, and Streptococcus equi ssp. zooepidemicus (SEZ) is one of the major pathogens. SEZ ATCC35246 is a classical virulent strain, while SEZ ST171 is a Chinese attenuated vaccine strain. In this study, we employed stable isotope labeling by amino acids in cell culture and liquid chromatography-mass spectrometry (LC-MS) to determine the differential response of macrophages to infection by these two strains. Eighty-seven upregulated proteins and 135 downregulated proteins were identified. The proteomic results were verified by real-time polymerase chain reaction for 10 chosen genes and Western blotting for three proteins. All differentially abundant proteins were analyzed for their Gene Ontology and Kyoto Encyclopedia of Genes and Genomes annotations. Certain downregulated proteins were associated with immunity functions, and the upregulated proteins were related to cytomembrane and cytoskeleton regulation. The phagocytosis rate and cytokine genes transcription in Raw264.7 cells during SEZ ATCC35246 and ST171 infection were detected to confirm the bioinformatics results. These results showed that different effects on macrophage phagocytosis and cytokine expression might explain the different phenotypes of SEZ ATCC35246 and ST171 infection. This research provided clues to the mechanisms of host immunity responses to SEZ ST171and SEZ ATCC35246, which could identify potential therapy and vaccine development targets.

Published
2016

46. SILAC and LC-MS/MS identification of Streptococcus equi ssp. zooepidemicus proteins that contribute to mouse brain microvascular endothelial cell infection

Author

Zhang Hui, Peng Jie, Fan Hong-jie, Xu Bin, Lu Chengping, Ma Zhe, Pei Xiaomeng, and Lin Huixing

Subjects
0301 basic medicine, Virulence Factors, Enolase, Virulence, Biology, Blood–brain barrier, Applied Microbiology and Biotechnology, Meningitis, Bacterial, Microbiology, Mice, 03 medical and health sciences, Bacterial Proteins, Tandem Mass Spectrometry, Streptococcal Infections, Stable isotope labeling by amino acids in cell culture, medicine, Animals, Humans, Streptococcus equi, Cytotoxic T cell, Cells, Cultured, chemistry.chemical_classification, Mice, Inbred BALB C, Brain, Endothelial Cells, General Medicine, In vitro, Amino acid, 030104 developmental biology, medicine.anatomical_structure, chemistry, Blood-Brain Barrier, Cytoplasm, Isotope Labeling, Female, Chromatography, Liquid, Biotechnology
Abstract

Streptococcus equi ssp. zooepidemicus (SEZ) causes meningitis in both humans and animals. Some dissociative proteins of SEZ are cytotoxic to mouse brain microvascular endothelial cells (mBMECs) and may contribute to the penetration of SEZ across the blood-brain barrier (BBB). In this study, the ability of SEZ to penetrate across an in vitro BBB model was confirmed. We used stable isotope labeling with amino acids in cell culture (SILAC) to label SEZ proteins with heavy or light isotope-tagged amino acids, along with LC-MS/MS to determine which SEZ proteins were involved in interactions with mBMECs. The efficiency of SEZ protein isotope labeling was 94.7 %, which was sufficient for further analysis. Forty-nine labeled peptides were identified as binding to mBMECs, which matched to 25 SEZ proteins. Bioinformatic analysis indicated that most of these proteins were cytoplasmic. These proteins may have functions in breaching the host BBB, and some of them are known virulence factors in other bacteria. Indirect immunofluorescence results indicated that SEZ enolase had binding activity toward mBMECs. Protective test results showed that enolase was a protective antigen against SEZ infection. This research is the first application of SILAC combined with LC-MS/MS to identify SEZ proteins that may contribute to the infection of mBMECs and potentially show functions related to breaching the BBB. The outcomes provide many future avenues for research into the mechanism of SEZ-induced meningitis.

Published
2016

49. Identification of six novel capsular polysaccharide loci ( NCL ) from Streptococcus suis multidrug resistant non‐typeable strains and the pathogenic characteristic of strains carrying new NCL s

Author

Huang, Jinhu, primary, Liu, Xi, additional, Chen, Hao, additional, Chen, Li, additional, Gao, Xueping, additional, Pan, Zihao, additional, Wang, Jian, additional, Lu, Chengping, additional, Yao, Huochun, additional, Wang, Liping, additional, and Wu, Zongfu, additional

Published
2019
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