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Your search keyword '"Lichtenegger, Sabine"' showing total 25 results
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25 results on '"Lichtenegger, Sabine"'

2. Melioidosis in Africa: Time to uncover the true disease load

Author

Steinmetz, Ivo, Wagner, Gabriel E, Kanyala, Estelle, Sawadogo, Mamadou, Soumeya, Hema, Teferi, Mekonnen, Andargie, Emawayish, Yeshitela, Biruk, Atse-Achi, Louise Yaba, Sanogo, Moussa, Bonfoh, Bassirou, Rakotozandrindrainy, Raphael, Shongo, Celestin Pongombo, Pongombo, Mick Shongoya, Ilunga, Eric Kasamba, Lichtenegger, Sabine, Assig, Karoline, May, Jurgen, Bertherat, Eric, Owusu, Michael, Owusu-Dabo, Ellis, and Adu-Sarkodie, Yaw

Published
2018

4. Newly detected paediatric melioidosis cases in a single referral children's hospital in Ho Chi Minh City indicate the probable underrecognition of melioidosis in South Vietnam

Author

Pham, Thai Son, primary, König, Elisabeth, additional, Bui, The Trung, additional, Vu, Thi Ngoc Anh, additional, Nguyen, Tran Nam, additional, Do, Chau Viet, additional, Lichtenegger, Sabine, additional, Bui, Nguyen Hai Linh, additional, Trinh, Huu Tung, additional, Steinmetz, Ivo, additional, and Trinh, Thanh Trung, additional

Published
2023
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5. Newly detected paediatric melioidosis cases in a single referral children's hospital in Ho Chi Minh City indicate the probable underrecognition of melioidosis in South Vietnam.

Author

Pham, Thai Son, König, Elisabeth, Bui, The Trung, Vu, Thi Ngoc Anh, Nguyen, Tran Nam, Do, Chau Viet, Lichtenegger, Sabine, Bui, Nguyen Hai Linh, Trinh, Huu Tung, Steinmetz, Ivo, and Trinh, Thanh Trung

Subjects
MELIOIDOSIS, CHILDREN'S hospitals, CITIES & towns, SOIL microbiology, BURKHOLDERIA pseudomallei
Abstract

Background The epidemiology of melioidosis in Vietnam, a disease caused by the soil bacterium Burkholderia pseudomallei , remains unclear. This study aimed to detect paediatric melioidosis in South Vietnam and describe clinical features and the geographic distribution. Methods We introduced a simple laboratory algorithm for detecting B. pseudomallei from clinical samples at Children's Hospital 2 in Ho Chi Minh City in July 2015. A retrospective observational study of children <16 y of age with culture-confirmed melioidosis between July 2015 and August 2019 was undertaken. Results Thirty-five paediatric cases of melioidosis were detected, with cases originating from 13 of 32 provinces and cities in South Vietnam. The number of paediatric melioidosis cases detected from a certain region correlated with the overall number of inpatients originating from the respective geographic area. Suppurative parotitis (n=15 [42.8%]) was the most common clinical presentation, followed by lung infection (n=10 [28.6%]) and septicaemia (n=7 [20%]). Fourteen (40%) children had disseminated disease, including all cases of lung infection, four cases with central nervous system symptoms and four (11.4%) deaths. Conclusions The patients' origin indicates a wide distribution of melioidosis in South Vietnam. It seems probable that cases not only in children, but also in adults, remain grossly undiagnosed. Further awareness raising and laboratory capacity strengthening are needed in this part of the country. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Real-Time Nanopore Q20+ Sequencing Enables Extremely Fast and Accurate Core Genome MLST Typing and Democratizes Access to High-Resolution Bacterial Pathogen Surveillance

Author

Wagner, Gabriel E., primary, Dabernig-Heinz, Johanna, additional, Lipp, Michaela, additional, Cabal, Adriana, additional, Simantzik, Jonathan, additional, Kohl, Matthias, additional, Scheiber, Martina, additional, Lichtenegger, Sabine, additional, Ehricht, Ralf, additional, Leitner, Eva, additional, Ruppitsch, Werner, additional, and Steinmetz, Ivo, additional

Published
2023
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9. Newly detected pediatric melioidosis cases in a single referral children’s hospital in Ho Chi Minh City indicate the probable under-recognition of melioidosis in South Vietnam

Author

Pham, Thai Son, primary, König, Elisabeth, additional, Bui, The Trung, additional, Vu, Thi Ngoc Anh, additional, Nguyen, Tran Nam, additional, Do, Chau Viet, additional, Lichtenegger, Sabine, additional, Bui, Nguyen Hai Linh, additional, Trinh, Huu Tung, additional, Steinmetz, Ivo, additional, and Trinh, Thanh Trung, additional

Published
2023
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11. Protein Microarray-Guided Development of a Highly Sensitive and Specific Dipstick Assay for Glanders Serodiagnostics

Author

Wagner, Gabriel E., primary, Berner, Andreas, additional, Lipp, Michaela, additional, Kohler, Christian, additional, Assig, Karoline, additional, Lichtenegger, Sabine, additional, Saqib, Muhammad, additional, Müller, Elke, additional, Trinh, Trung T., additional, Gad, Anne-Marie, additional, Söffing, Hans Hermann, additional, Ehricht, Ralf, additional, Laroucau, Karine, additional, and Steinmetz, Ivo, additional

Published
2023
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12. Development of a Rapid Live SARS-CoV-2 Neutralization Assay Based on a qPCR Readout

Author

Lichtenegger, Sabine, primary, Saiger, Sabine, additional, Hardt, Melina, additional, Kulnik, Susanne, additional, Wagner, Gabriel E., additional, Kleinhappl, Barbara, additional, Assig, Karoline, additional, Zauner, Andrea, additional, Ober, Michelle, additional, Kimpel, Janine, additional, von Laer, Dorothee, additional, Zatloukal, Kurt, additional, and Steinmetz, Ivo, additional

Published
2022
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15. Development and Validation of a Burkholderia pseudomallei Core Genome Multilocus Sequence Typing Scheme To Facilitate Molecular Surveillance

Author

Lichtenegger, Sabine, primary, Trinh, Trung T., additional, Assig, Karoline, additional, Prior, Karola, additional, Harmsen, Dag, additional, Pesl, Julian, additional, Zauner, Andrea, additional, Lipp, Michaela, additional, Que, Tram A., additional, Mutsam, Beatrice, additional, Kleinhappl, Barbara, additional, Steinmetz, Ivo, additional, and Wagner, Gabriel E., additional

Published
2021
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17. Burkholderia pseudomallei triggers canonical inflammasome activation in a human primary macrophage-based infection model

Author

Lichtenegger, Sabine, primary, Stiehler, Julia, additional, Saiger, Sabine, additional, Zauner, Andrea, additional, Kleinhappl, Barbara, additional, Bernecker, Claudia, additional, Schlenke, Peter, additional, Wagner, Gabriel E., additional, Krause, Kathrin, additional, Gastager, Magdalena, additional, and Steinmetz, Ivo, additional

Published
2020
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18. Melioidosis DS rapid test: A standardized serological dipstick assay with increased sensitivity and reliability due to multiplex detection

Author

Wagner, Gabriel E., primary, Föderl-Höbenreich, Esther, additional, Assig, Karoline, additional, Lipp, Michaela, additional, Berner, Andreas, additional, Kohler, Christian, additional, Lichtenegger, Sabine, additional, Stiehler, Julia, additional, Karoonboonyanan, Wisansanee, additional, Thanapattarapairoj, Nida, additional, Promkong, Chidchanok, additional, Koosakulnirand, Sirikamon, additional, Chaichana, Panjaporn, additional, Ehricht, Ralf, additional, Gad, Anne-Marie, additional, Söffing, Hans H., additional, Dunachie, Susanna J., additional, Chantratita, Narisara, additional, and Steinmetz, Ivo, additional

Published
2020
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19. Erythritol as a single carbon source improves cultural isolation of Burkholderia pseudomallei from rice paddy soils

Author

Trinh, Trung T., primary, Assig, Karoline, additional, Tran, Quyen T. L., additional, Goehler, André, additional, Bui, Linh N. H., additional, Wiede, Claudia, additional, Folli, Bettina, additional, Lichtenegger, Sabine, additional, Nguyen, Tinh T., additional, Wagner, Gabriel E., additional, Kohler, Christian, additional, and Steinmetz, Ivo, additional

Published
2019
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20. Increased Aggregation Tendency of Alpha-Synuclein in a Fully Disordered Protein Complex

Author

Merle, David A., primary, Witternigg, Anja, additional, Tam-Amersdorfer, Carmen, additional, Hartlmüller, Christoph, additional, Spreitzer, Emil, additional, Schrank, Evelyne, additional, Wagner-Lichtenegger, Sabine, additional, Werzer, Oliver, additional, Zangger, Klaus, additional, Kungl, Andreas J., additional, Madl, Tobias, additional, Meyer, N. Helge, additional, and Falsone, S. Fabio, additional

Published
2019
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21. A cation–π interaction in a transmembrane helix of vacuolar ATPase retains the proton-transporting arginine in a hydrophobic environment

Author

Hohlweg, Walter, primary, Wagner, Gabriel E., additional, Hofbauer, Harald F., additional, Sarkleti, Florian, additional, Setz, Martina, additional, Gubensäk, Nina, additional, Lichtenegger, Sabine, additional, Falsone, Salvatore Fabio, additional, Wolinski, Heimo, additional, Kosol, Simone, additional, Oostenbrink, Chris, additional, Kohlwein, Sepp D., additional, and Zangger, Klaus, additional

Published
2018
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22. A combined vaccine approach against Vibrio cholerae and ETEC based on outer membrane vesicles

Author

Leitner, Deborah R., Lichtenegger, Sabine, Temel, Philipp, Zingl, Franz G., Ratzberger, Desiree, Roier, Sandro, Schild-Prüfert, Kristina, Feichter, Sandra, Reidl, Joachim, and Schild, Stefan

Subjects
Microbiology (medical), Vaccines, lcsh:QR1-502, bacteria, Enterotoxigenic Escherichia coli, Public Health, enteric pathogens, Microbiology, Vibrio cholerae, outer membrane vesicles, lcsh:Microbiology, Original Research, combined vaccine
Abstract

Enteric infections induced by pathogens like Vibrio cholerae and enterotoxigenic Escherichia coli (ETEC) remain a massive burden in developing countries with increasing morbidity and mortality rates. Previously, we showed that the immunization with genetically detoxified outer membrane vesicles (OMVs) derived from V. cholerae elicits a protective immune response based on the generation of O antigen antibodies, which effectively block the motility by binding to the sheathed flagellum. In this study, we investigated the potential of lipopolysaccharide (LPS)-modified and toxin negative OMVs isolated from V. cholerae and ETEC as a combined OMV vaccine candidate. Our results indicate that the immunization with V. cholerae or ETEC OMVs induced a species-specific immune response, whereas the combination of both OMV species resulted in a high-titer, protective immune response against both pathogens. Interestingly, the immunization with V. cholerae OMVs alone resulted in a so far uncharacterized and cholera toxin B-subunit (CTB) independent protection mechanism against an ETEC colonization. Furthermore, we investigated the potential use of V. cholerae OMVs as delivery vehicles for the heterologously expression of the ETEC surface antigens, CFA/I, and FliC. Although we induced a detectable immune response against both heterologously expressed antigens, none of these approaches resulted in an improved protection compared to a simple combination of V. cholerae and ETEC OMVs. Finally, we expanded the current protection model from V. cholerae to ETEC by demonstrating that the inhibition of motility via anti-FliC antibodies represents a relevant protection mechanism of an OMV-based ETEC vaccine candidate in vivo.

Published
2015

23. Outer membrane protein P1 is the CEACAM-binding adhesin of Haemophilus influenzae

Author

Kengmo Tchoupa, Arnaud, Lichtenegger, Sabine, Reidl, Joachim, and Hauck, Christof R.

Subjects
ddc:570
Abstract

Haemophilus influenzae is a Gram-negative pathogen colonizing the upper respiratory tract mucosa. H. influenzae is one of several human-restricted bacteria, which bind to carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) on the epithelium leading to bacterial uptake by the eukaryotic cells. Adhesion to CEACAMs is thought to be mediated by the H. influenzae outer membrane protein (OMP) P5. However, CEACAMs still bound to H. influenzae lacking OMP P5 expression, and soluble CEACAM receptor ectodomains failed to bind to OMP P5, when heterologously expressed in Escherichia coli. Screening of a panel of H. influenzae OMP mutants revealed that lack of OMP P1 completely abrogated CEACAM binding and supressed CEACAM-mediated engulfment of H. influenzae by epithelial cells. Moreover, ectopic expression of OMP P1 in E. coli was sufficient to induce CEACAM binding and to promote attachment to and internalization into CEACAM-expressing cells. Interestingly, OMP P1 selectively recognizes human CEACAMs, but not homologs from other mammals and this binding preference is preserved upon expression in E. coli. Together, our data identify OMP P1 as the bona fide CEACAM-binding invasin of H. influenzae. This is the first report providing evidence for an involvement of the major OMP P1 of H. influenzae in pathogenesis. published

Published
2015

24. Outer membrane protein P1 is the CEACAM-binding adhesin of H aemophilus influenzae.

Author

Tchoupa, Arnaud Kengmo, Lichtenegger, Sabine, Reidl, Joachim, and Hauck, Christof R.

Subjects
*HAEMOPHILUS influenzae, *MEMBRANE proteins, *VIRAL adhesins, *CELL adhesion molecules, *EUKARYOTIC cells
Abstract

H aemophilus influenzae is a Gram-negative pathogen colonizing the upper respiratory tract mucosa. H . influenzae is one of several human-restricted bacteria, which bind to carcinoembryonic antigen-related cell adhesion molecules ( CEACAMs) on the epithelium leading to bacterial uptake by the eukaryotic cells. Adhesion to CEACAMs is thought to be mediated by the H . influenzae outer membrane protein ( OMP) P5. However, CEACAMs still bound to H . influenzae lacking OMP P5 expression, and soluble CEACAM receptor ectodomains failed to bind to OMP P5, when heterologously expressed in E scherichia coli. Screening of a panel of H . influenzae OMP mutants revealed that lack of OMP P1 completely abrogated CEACAM binding and supressed CEACAM-mediated engulfment of H . influenzae by epithelial cells. Moreover, ectopic expression of OMP P1 in E . coli was sufficient to induce CEACAM binding and to promote attachment to and internalization into CEACAM-expressing cells. Interestingly, OMP P1 selectively recognizes human CEACAMs, but not homologs from other mammals and this binding preference is preserved upon expression in E . coli. Together, our data identify OMP P1 as the bona fide CEACAM-binding invasin of H . influenzae. This is the first report providing evidence for an involvement of the major OMP P1 of H . influenzae in pathogenesis. [ABSTRACT FROM AUTHOR]

Published
2015
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25. Development and Validation of a Burkholderia pseudomalleiCore Genome Multilocus Sequence Typing Scheme To Facilitate Molecular Surveillance

Author

Lichtenegger, Sabine, Trinh, Trung T., Assig, Karoline, Prior, Karola, Harmsen, Dag, Pesl, Julian, Zauner, Andrea, Lipp, Michaela, Que, Tram A., Mutsam, Beatrice, Kleinhappl, Barbara, Steinmetz, Ivo, and Wagner, Gabriel E.

Abstract

Burkholderia pseudomalleicauses the severe disease melioidosis. Whole-genome sequencing (WGS)-based typing methods currently offer the highest resolution for molecular investigations of this genetically diverse pathogen.

Published
2021
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