Search

This work deals with the image capture for close-range photogrammetry in the context of the modelling of a round tower with repetitive texture (made of bricks), both inside and outside. For that purpose we test different acquisition strategies that differ in terms of camera path (straight or circular) and number of images acquired at each camera position, that is with or without oblique images. Besides, the formula to compute the curvilinear base according to a given overlap rate between successive frontal photos are written for each strategy. The comparisons rely on the observation of the aspect of the different dense point clouds (noise, holes), the deformation seen in the orthomosaics and values extracted from the photogrammetric projects such as metric accuracies with GCP. Our results confirm some art rules. [ABSTRACT FROM AUTHOR]

The sugar kelp Saccharina latissima is cultivated in Europe for food, feed and ultimately the production of chemical commodities and bioenergy. Being cultivated in the open sea, S. latissima is exposed to potentially harmful organisms, such as Laminarionema elsbetiae, a filamentous brown algal endophyte with a very high prevalence in wild populations of European S. latissima. As it was shown previously that S. latissima sporophytes get infected by L. elsbetiae very early in their life, seeding the spores on collectors and keeping them under controlled conditions during the critical time of a possible infection with filamentous endophytes could be advantageous over direct seeding techniques, where the ropes are deployed within days after seeding. We used a qPCR-assay to assess the prevalence of the endophyte L. elsbetiae in S. latissima cultivated during winter in Northern Brittany, comparing individuals from direct-seeded ropes and collector-seeded lines that were kept in laboratory conditions for different time spans. No DNA of the endophyte was detected in the samples, suggesting that either the kelps were not infected or the amount of endophytic filaments were below the detection rate of the qPCR assay. Furthermore, L. elsbetiae could not be detected in the seawater surrounding the kelp farm, indicating that L. elsbetiae is not fertile or disperses at a very small scale in Northern Brittany during the deployment time of young kelps. Our results suggest that infections of cultivated S. latissima with the endophyte L. elsbetiae might be a minor problem in kelp farms in Northern Brittany if the seeding production is kept under controlled conditions without external contamination.

We model the nonlinear response of a lubricated contact composed of a two-dimensional lipid membrane immersed in a simple fluid between two parallel flat and porous walls under shear. The nonlinear dynamics of the membrane gives rise to a rich dynamical behavior depending on the shear velocity. In quiescent conditions (i.e., absence of shear), the membrane freezes into a disordered labyrinthine wrinkle pattern. We determine the wavelength of this pattern as a function of the excess area of the membrane for a fairly general form of the confinement potential using a sine-profile ansatz for the wrinkles. In the presence of shear, we find four different regimes depending on the shear rate. Regime I. For small shear, the labyrinthine pattern is still frozen, but exhibits a small drift which is mainly along the shear direction. In this regime, the tangential forces on the walls due to the presence of the membrane increase linearly with the shear rate. Regime II. When the shear rate is increased above a critical value, the membrane rearranges, and wrinkles start to align along the shear direction. This regime is accompanied by a sharp drop of the tangential forces on the wall. The membrane usually reaches a steady-state configuration drifting with a small constant velocity at long times. However, we also rarely observe oscillatory dynamics in this regime. Regime III. For larger shear rates, the wrinkles align strongly along the shear direction, with a set of dislocation defects which assemble in pairs. The tangential forces are then controlled by the number of dislocations, and by the number of wrinkles between the two dislocations within each dislocation pairs. In this dislocation-dominated regime, the tangential forces in the transverse direction most often exceed those in the shear direction. Regime IV. For even larger shear, the membrane organizes into a perfect array of parallel stripes with no defects. The wavelength of the wrinkles is still identical to the wavelength in the absence of shear. In this final regime, the tangential forces due to the membrane vanish. These behaviors give rise to a non-linear rheological behavior of lubricated contacts containing membranes.

Cell crawling on two-dimensional surfaces is a relatively well-understood phenomenon that is based on actin polymerization at a cell's front edge and anchoring on a substrate, allowing the cell to pull itself forward. However, some cells, such as cancer cells invading a three-dimensional matrigel, can also swim in the bulk, where surface adhesion is impossible. Although there is strong evidence that the self-organized engine that drives cells forward in the bulk involves myosin, the specific propulsion mechanism remains largely unclear. Here, we propose a minimal model for in-bulk self-motility of a droplet containing an isotropic and compressible contractile gel, representing a cell extract containing a disordered actomyosin network. In our model, contraction mediates a feedback loop between myosin-induced flow and advection-induced myosin accumulation, which leads to clustering and locally enhanced flow. The symmetry of such flow is then spontaneously broken through actomyosin-membrane interactions, leading to self-organized droplet motility relative to the underlying solvent. Depending on the balance between contraction, diffusion, detachment rate of myosin, and effective surface tension, this motion can be either straight or circular. Our simulations and analytical results shed new light on in-bulk myosin-driven cell motility in living cells and provide a framework to design a novel type of synthetic active matter droplet potentially resembling the motility mechanism of biological cells., (Copyright © 2020 Biophysical Society. Published by Elsevier Inc. All rights reserved.)

We report on the modeling of the dynamics of confined lipid membranes. We derive a thin film model in the lubrication limit which describes an inextensible liquid membrane with bending rigidity confined between two adhesive walls. The resulting equations share similarities with the Swift-Hohenberg model. However, inextensibility is enforced by a time-dependent nonlocal tension. Depending on the excess membrane area available in the system, three different dynamical regimes, denoted as A, B and C, are found from the numerical solution of the model. In regime A, membranes with small excess area form flat adhesion domains and freeze. Such freezing is interpreted by means of an effective model for curvature-driven domain wall motion. The nonlocal membrane tension tends to a negative value corresponding to the linear stability threshold of flat domain walls in the Swift-Hohenberg equation. In regime B, membranes with intermediate excess areas exhibit endless coarsening with coexistence of flat adhesion domains and wrinkle domains. The tension tends to the nonlinear stability threshold of flat domain walls in the Swift-Hohenberg equation. The fraction of the system covered by the wrinkle phase increases linearly with the excess area in regime B. In regime C, membranes with large excess area are completely covered by a frozen labyrinthine pattern of wrinkles. As the excess area is increased, the tension increases and the wavelength of the wrinkles decreases. For large membrane area, there is a crossover to a regime where the extrema of the wrinkles are in contact with the walls. In all regimes after an initial transient, robust localised structures form, leading to an exact conservation of the number of adhesion domains.

We have modeled the nonlinear dynamics and the rheological behavior of a system under shear containing a membrane confined between two attractive walls. The presence of the membrane induces additional tangential forces on the walls that always increase the global friction. At low shear rates, the membrane exhibits chaotic dynamics with slow coarsening leading to thixotropy, i.e. to a slow decrease of the membrane-induced tangential forces on the walls. At intermediate shear rates, the membrane profile presents stationary periodic patterns. At higher shear rates, membrane dynamics are governed by a nonlinear evolution equation which is similar to the Kuramoto-Sivashinski equation, but with a sixth-order stabilizing term. The membrane experiences chaotic dynamics without coarsening. As a consequence of the nonlinear dynamics of the membrane at intermediate and large shear rates, the system exhibits shear thinning.

We discuss the nonlinear dynamics and fluctuations of interfaces with bending rigidity under the competing attractions of two walls with arbitrary permeabilities. This system mimics the dynamics of confined membranes. We use a two-dimensional hydrodynamic model, where membranes are effectively one-dimensional objects. In a previous work [T. Le Goff et al., Phys. Rev. E 90, 032114 (2014)], we have shown that this model predicts frozen states caused by bending rigidity-induced oscillatory interactions between kinks (or domain walls). We here demonstrate that in the presence of tension, potential asymmetry, or thermal noise, there is a finite threshold above which frozen states disappear, and perpetual coarsening is restored. Depending on the driving force, the transition to coarsening exhibits different scenarios. First, for membranes under tension, small tensions can only lead to transient coarsening or partial disordering, while above a finite threshold, membrane oscillations disappear and perpetual coarsening is found. Second, potential asymmetry is relevant in the nonconserved case only, i.e., for permeable walls, where it induces a drift force on the kinks, leading to a fast coarsening process via kink-antikink annihilation. However, below some threshold, the drift force can be balanced by the oscillatory interactions between kinks, and frozen adhesion patches can still be observed. Finally, at long times, noise restores coarsening with standard exponents depending on the permeability of the walls. However, the typical time for the appearance of coarsening exhibits an Arrhenius form. As a consequence, a finite noise amplitude is needed in order to observe coarsening in observable time.