178 results on '"Kurpińska A"'
Search Results
2. Investigating the suitability of the matched fiber Bragg grating approach for guided wave based structural health monitoring
- Author
-
Soman, Rohan, Moaf, Farzam Omidi, Fiborek, Piotr, Kudela, Pawel, and Kurpińska, Marzena
- Published
- 2025
- Full Text
- View/download PDF
3. Protein disulfide isomerase A1 regulates fenestration dynamics in primary mouse liver sinusoidal endothelial cells (LSECs)
- Author
-
Czyzynska-Cichon, Izabela, Giergiel, Magdalena, Kwiatkowski, Grzegorz, Kurpinska, Anna, Wojnar-Lason, Kamila, Kaczara, Patrycja, Szymonski, Marek, Lekka, Malgorzata, Kalvins, Ivars, Zapotoczny, Bartlomiej, and Chlopicki, Stefan
- Published
- 2024
- Full Text
- View/download PDF
4. Machine learning-based prediction of preplaced aggregate concrete characteristics
- Author
-
Moaf, Farzam Omidi, Kazemi, Farzin, Abdelgader, Hakim S., and Kurpińska, Marzena
- Published
- 2023
- Full Text
- View/download PDF
5. Fire-induced spalling of ultra-high performance concrete: A systematic critical review
- Author
-
Amran, Mugahed, Murali, G., Makul, Natt, Kurpińska, Marzena, and Nehdi, Moncef L.
- Published
- 2023
- Full Text
- View/download PDF
6. The impact of natural fibers’ characteristics on mechanical properties of the cement composites
- Author
-
Marzena Kurpińska, Magdalena Pawelska-Mazur, Yining Gu, and Filip Kurpiński
- Subjects
Medicine ,Science - Abstract
Abstract The paper reviews the properties of cement composites reinforced with short fibres. The effect of natural fibres was investigated: cotton, sisal, jute, ramie, bamboo, and synthetic fibres: polymer and polypropylene. It was noticed that the fibres change the consistency of the mixture up to 15%. In the composite flexural strength tests, a change in strength by +/− 8% was observed, depending on the type of fibres used. The research shows that the use of natural fibres had a positive effect on the compressive strength by 27%, while the use of synthetic fibres caused its decrease by 4%. Additionally, it was noticed that the chemical composition, the diameter and the total length of the fibres in the element have an impact on the composite shrinkage. The fibre-containing composites showed an 8% higher water absorption compared to the non-fibre samples. The exception is the ramie fibres, which reduce water absorption. In general, a positive effect of natural fibers on the properties of cement composites has been noticed, however, in case of natural fibres application, a thorough further properties investigation is recommended.
- Published
- 2022
- Full Text
- View/download PDF
7. Comparison of anti-cancer effects of novel protein disulphide isomerase (PDI) inhibitors in breast cancer cells characterized by high and low PDIA17 expression
- Author
-
Anna Kurpińska, Joanna Suraj-Prażmowska, Marta Stojak, Joanna Jarosz, Łukasz Mateuszuk, Ewa Niedzielska-Andres, Magdalena Smolik, Joanna Wietrzyk, Ivars Kalvins, Maria Walczak, and Stefan Chłopicki
- Subjects
Protein disulphide isomerases ,Anterior gradient 2 (AGR2, PDIA17) ,Novel PDI inhibitors ,Endoplasmic reticulum (ER)–resident protein 57 (ERP57, PDIA3) ,Protein disulphide isomerase A1 (prolyl 4-hydroxylase subunit beta ,P4HB, PDIA1) ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Protein disulphide isomerases (PDIs) play an important role in cancer progression. However, the relative contribution of the various isoforms of PDI in tumorigenesis is not clear. Methods The content of PDI isoforms in 22 cancer cells lines was investigated using LC–MS/MS-based proteomic analysis. The effects of PDIA1, PDIA3 and PDIA17 inhibition on the proliferation, migration and adhesion of MCF-7 and MDA-MB-231 cells, identified as high and low PDIA17 expressing cells, respectively, were assessed using novel aromatic N-sulphonamides of aziridine-2-carboxylic acid derivatives as PDI inhibitors. Results PDIA1 and PDIA3 were the most abundant in cancer cell lysates and were also detected extracellularly in breast cancer cells (MDA-MB-231 and MCF-7). Some cancer cell lines (e.g., MCF-7, HT-29) showed upregulated expression of PDIA17, whereas in others (e.g., MDA-MB-231, 67NR), PDIA17 was not detected. The simultaneous inhibition of PDIA1 and PDIA3 showed similar anti-proliferative effects in MCF-7 and MDA-MB-231 breast cancer cells. However, the inhibition of PDIA1 and PDIA17 in the MCF-7 cell line resulted in more effective anti-adhesive and anti-proliferative effects. Conclusions PDIA1 and PDIA3 represent major isoforms of multiple cancer cells, and their non-selective inhibition displays significant anti-proliferative effects irrespective of whether or not PDIA17 is present. The more pronounced anti-adhesive effects of PDI inhibition in hormone-sensitive MCF-7 cells featured by higher levels of PDIs when compared to triple-negative MDA-MB-231 cells suggests that targeting extracellular PDIA1 and PDIA3 with or without additional PDIA17 inhibition may represent a strategy for personalized anti-adhesive, anti-metastatic therapy in cancers with high PDI expression.
- Published
- 2022
- Full Text
- View/download PDF
8. Assessment of the application of CEM III with exposed aggregate as an alternative to CEM I for road pavements
- Author
-
Marzena Kurpińska and Aldona Wcisło
- Subjects
road pavement ,concrete ,exposed aggregate ,compressive strength ,tensile strength ,cement ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The article presents a results of study on the impact of replacing CEM I SR3/NA by CEMIII/A LH/HSR/NAon the mechanical properties and durability of pavement concrete with exposed aggregate. Was used granite aggregate and washed sand. Water/cement (w / c) ratio in the tested concretes constituted 0.35 and 0.4 and part of the cement was replaced with a 5% addition of natural pozzolana – zeolite. Compressive strength tests were performed after 3, 7, 28 and 56 days, tests of tensile strength test by splitting method and flexural strength two-point loading tests. The characteristics of the air pores and the rate of water absorption by concrete surface of the samples cut out from the slabs with exposed aggregate were presented. The resistance of the surface to exfoliation after 56 cycles of freezing-thawing in NaCl solution was tested. Based on the results obtained, it was found that when designing the composition of the concrete intended for the upper layer of the pavement, it is necessary to ensure high tensile strength, appropriate in the XF4 environment and with the decrease in the w / c < 0.4, a reduction in capillary porosity of the cement paste is obtained, and the same the durability of concrete is increased due to the improved strength parameters in the contact zone between coarse aggregate grains and cement paste. The research also showed a significant influence of proper cure on the mechanical properties and durability of pavement concrete.
- Published
- 2022
- Full Text
- View/download PDF
9. Effect of slag coal ash and foamed glass on the mechanical properties of two-stage concrete
- Author
-
Abdelgader, Hakim S., Kurpińska, Marzena, and Amran, Mugahed
- Published
- 2022
- Full Text
- View/download PDF
10. Protein disulfide isomerase‐A1 regulates intraplatelet reactive oxygen species–thromboxane A2‐dependent pathway in human platelets
- Author
-
Przyborowski, Kamil, Kurpinska, Anna, Wojkowska, Dagmara, Kaczara, Patrycja, Suraj‐Prazmowska, Joanna, Karolczak, Kamil, Malinowska, Agata, Pelesz, Agnieszka, Kij, Agnieszka, Kalvins, Ivars, Watala, Cezary, and Chlopicki, Stefan
- Published
- 2022
- Full Text
- View/download PDF
11. COVID-19-related social isolation and symptoms of depression and anxiety in young men in Poland: Does insomnia mediate the relationship?
- Author
-
Justyna Mojsa-Kaja, Klaudia Szklarczyk-Smolana, Ewa Niedzielska-Andres, Anna Kurpińska, Joanna Suraj-Prażmowska, and Maria Walczak
- Subjects
Medicine ,Science - Abstract
The need for physical distancing due to COVID-19 mitigation efforts forced prolonged social isolation, which may affect sleep and lead to mental health problems. Previous research has shown that young adults are particularly vulnerable to psychological stress caused by social isolation, the negative psychological impact of the pandemic, and greater frequency and severity of sleep problems. Therefore, the main goal of the present study was to examine whether insomnia could constitute a mediation mechanism that explains the relationship between social isolation experienced during the COVID-19 pandemic and mental health outcomes (depression and anxiety) reported up to 1.5 years later. The study was conducted among young (M±SD; 24.08±3.75) men (N = 1025) in Poland. Data were collected by means of self-report questionnaires, including The Social Isolation Index, The Athens Insomnia Scale, The State-Trait Anxiety Inventory (STAI-S) and Beck's Depression Inventory (BDI-II). The results show that insomnia mediates the relationships between social isolation and both anxiety and depression. The current findings emphasize the role of insomnia in the relationships between social isolation experienced during COVID-19 and negative emotional states. From a clinical perspective, the results suggest that implementing therapeutic components that address social isolation in insomnia treatment programs may prevent the development of depression and anxiety symptoms among young men.
- Published
- 2023
- Full Text
- View/download PDF
12. Characterization of fracture process in polyolefin fibre-reinforced concrete using ultrasonic waves and digital image correlation
- Author
-
Rucka, Magdalena, Wojtczak, Erwin, Knak, Magdalena, and Kurpińska, Marzena
- Published
- 2021
- Full Text
- View/download PDF
13. Cement kiln dust
- Author
-
Abdelgader, Hakim S., primary, Amran, Mugahed, additional, Kurpińska, Marzena, additional, Mosaberpanah, Mohammad A., additional, Murali, Gunasekaran, additional, and Fediuk, Roman, additional
- Published
- 2022
- Full Text
- View/download PDF
14. Contributors
- Author
-
Abdelgader, Hakim S., primary, Ameri, Farshad, additional, Amran, Mugahed, additional, Aygün, Beyza Fahriye, additional, Barros, Regina Mambeli, additional, Bilir, Turhan, additional, Cheah, Chee Ban, additional, Contrafatto, Loredana, additional, Demirboga, Ramazan, additional, Duan, Weiwei, additional, Farhan, Khatib Zada, additional, Fediuk, Roman, additional, Filho, Romildo Dias Toledo, additional, Fontes, Cintia Maria Ariani, additional, Gupta, Nikita, additional, Hossain, Zahid, additional, Islam, Kazi Tamzidul, additional, Karadağ, Ömer, additional, Khavarian, Mehrnoush, additional, Kim, Hyeong-Ki, additional, Kurpińska, Marzena, additional, Lim, Jay Sern, additional, Liu, Yue, additional, Mosaberpanah, Mohammad A., additional, Murali, Gunasekaran, additional, Siddique, Rafat, additional, Singh, Jagdeep, additional, Singh, Malkit, additional, Tang, Waiching, additional, Yousefi, Ali, additional, and Zhuge,, Yan, additional
- Published
- 2022
- Full Text
- View/download PDF
15. Difficult diagnosis and surgical treatment of acute aortic syndrome
- Author
-
Wioleta Kurpińska-Kac, Jakub Osowski, Natalia Sobczyk, and Andrzej Łoś
- Subjects
Surgery ,RD1-811 ,Internal medicine ,RC31-1245 - Abstract
Acute aortic syndrome (AAS) continues to be a major clinical problem due to the need for urgent diagnosis and complex surgical management. We present a case that was not only surgically demanding, but also caused many difficulties in the preoperative diagnostic process. Typical clinical symptoms of AAS did not correlate with imaging study results, including computed tomography (CT) angiography and transthoracic echocardiography (TTE). A 64-year-old female patient was admitted to the hospital due to tearing, excruciating chest pain lasting for 18 hours and radiating to the interscapular area. Pain tended to slightly decrease with time. Other symptoms were transient numbness of the left lower extremity (a few hours) and amblyopia, lasting for several minutes. She had a history of poorly controlled hypertension, nicotinism, nephrectomy due to renal cell carcinoma, peptic ulcer disease, anxiety, depressive disorder, and addiction to clonazepam.
- Published
- 2022
- Full Text
- View/download PDF
16. Synovial sarcoma of the stomach: case report and systematic review of the literature
- Author
-
Martyna Kurpińska, Ewa Kaznowska, Anna Kruczak, Katarzyna Mularz, Agnieszka Adamczyk, Jarosław Długosz, Józef Gancarz, and Janusz Ryś
- Subjects
synovial sarcoma ,stomach ,spindle cell neoplasm ,cytokeratin. ,Medicine - Abstract
Synovial sarcoma is a rare mesenchymal malignant neoplasm that presents a specific t(X;18) translocation forming SS18(SYT)-SSX chimera gene. It is most commonly seen in soft tissues of the extremities. The digestive tract is an exceptional site of involvement. We report a case of primary gastric synovial sarcoma in a 48-year-old female. Differential diagnosis of synovial sarcoma from other spindle cell, mesenchymal and cytokeratin-positive tumors is critical for the treatment and prognosis. Immunohistochemistry studies and molecular analysis are required to settle a proper diagnosis.
- Published
- 2020
- Full Text
- View/download PDF
17. A Study on Fibre-Reinforced Concrete Elements Properties Based on the Case of Habitat Modules in the Underwater Sills
- Author
-
Kurpińska Marzena, Grzyl Beata, and Kristowski Adam
- Subjects
hydrotechnical constructions ,fibre-concrete ,mechanical concrete ,strength ,x-ray tomography ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 - Abstract
Hydrotechnical constructions are mostly objects functioning in extreme conditions and requiring a custom-made construction project. In the case of using prefabricated elements, it is required to develop production, transport, assembly, conservation and repair technology. Concerning the problem of concrete cracks, modern repair systems allow positive effects to be achieved in many cases of concrete elements repair. In this work an attempt has been made to assess the properties of concrete, situated in the Baltic Sea environment, in which traditional rebar was partly replaced by dispersed fibre-phase. Fibre-reinforced concrete belongs to the group of composite materials. The presence of fibres helps to increase the tensile strength, flexural strength and resilience and also prevents the appearance of cracks. In the given paper we will also discuss basic parameters of steel and polymer fibres and the influence of both types of fibres on the maturing and hardened concrete. In this work special attention has been paid to the advantages of polypropylene and polymer fibres with regard to commonly-known steel fibres. The use of synthetic fibres will be advantageous in constructions where the reduction of shrinkage cracks and high resilience are essential. On top of that, the use of synthetic fibres is highly recommended when constructing objects that will be exposed to the impact of an aggressive environment. Undoubtedly, polymer fibres are resistant to the majority of corrosive environments. Fibre-reinforced concretes are a frequently implemented construction solution. The possibility of concrete modification allows the emergence of new construction materials with improved physical-mechanical properties, under the condition of being applied relevantly.
- Published
- 2020
- Full Text
- View/download PDF
18. Proteomic characterization of early lung response to breast cancer metastasis in mice
- Author
-
Kurpińska, Anna, Suraj, Joanna, Bonar, Emilia, Zakrzewska, Agnieszka, Stojak, Marta, Sternak, Magdalena, Jasztal, Agnieszka, and Walczak, Maria
- Published
- 2019
- Full Text
- View/download PDF
19. Quantitative measurement of selected protein biomarkers of endothelial dysfunction in plasma by micro-liquid chromatography-tandem mass spectrometry based on stable isotope dilution method
- Author
-
Suraj, Joanna, Kurpińska, Anna, Sternak, Magdalena, Smolik, Magdalena, Niedzielska-Andres, Ewa, Zakrzewska, Agnieszka, Sacha, Tomasz, Kania, Aleksander, Chlopicki, Stefan, and Walczak, Maria
- Published
- 2019
- Full Text
- View/download PDF
20. The lethal effect of hydrotechnical concrete on freshwater Bivalvia
- Author
-
Wojtasik Barbara, Zbawicka Małgorzata, Grabarczyk Lucyna, and Kurpińska Marzena
- Subjects
hydrotechnical buildings ,concrete admixtures ,freshwater reservoirs ,dreissena polymorpha ,toxic ,Physical geography ,GB3-5030 ,Environmental engineering ,TA170-171 ,Technology - Abstract
Most hydrotechnical buildings under construction demand the concrete mixture to be set directly under water. The main reason for such a procedure is to limit the washing away of the the concrete binding mixture and to increase the efficiency of organisation of work so as to ensure continuity in concreting. The impact on the aquatic environment of recent developments in concrete technology and the use of new components has not yet been established. Natural pebble aggregate containing portland cement and fugacious siliceous ash as a binder was used to prepare BP concrete samples, while concrete marked LB was composed with lightweight aggregate and portland cement as a binder. The aim of this paper was to answer to the question whether hydrotechnical concrete of different compositions (BP and LB) and the technology of setting in a water habitat have any influence on the life condition of commonly occurring Dreissena polymorpha (Mollusca, Bivalvia). The lethal effect of two types of freshly hardening concrete was observed. In the case of LB concrete the lethal outcome for D. polymorpha could be the effect of a considerable increase of electrolytic conduction in the test cultivation. In the case of BP the parameters of electrolytic conductivity and pH did not exceed the values appearing in lakes. The possibility of the occurrence of toxic compounds of D. polymorpha, arising from the reaction of the aquatic / lake environment or the elution of some components should be taken into account. D. polymorpha serves as an indicator of toxicity in the aquatic environment and therefore can be used as a model organism in the analysis of the influence concrete on the natural environment. The results obtained in this study indicate the significant impact of modern chemical composition of concrete on the aquatic environment and the living organisms that cover it. They underline the need for research based on the hydrobiont reaction to the substances used in the natural environment.
- Published
- 2019
- Full Text
- View/download PDF
21. Assessment of changes in the occurrence of Fusarium toxin and ochratoxin A in Poland related to extreme weather phenomena
- Author
-
Jacek Postupolski, Andrzej Starski, Ewa Ledzion, Jolanta Kurpińska-Jaworska, and Małgorzata Szczęsna
- Subjects
mycotoxins ,cereal products ,formation ,weather condition ,prediction ,Fusarium toxin ,ochratoxin A ,Nutrition. Foods and food supply ,TX341-641 ,Industrial medicine. Industrial hygiene ,RC963-969 ,Public aspects of medicine ,RA1-1270 - Abstract
Background. Mycotoxins – substances harmful to humans, are ubiquitous in the environment. Mycotoxins are generated primarily by Penicilium, Aspergillus and Fusarium genus fungi. Their presence is associated with the unavoidable presence of mold fungi in the environment. The presently observed adverse climatic changes could negatively affect agriculture, causing erosion and loss of organic matter from soil, promulgation of pests and plant diseases, including those originating from pathogenic molds, and also migration of certain mold species into new regions, ultimately creating more favorable conditions for generation of mycotoxins. Objective. The purpose of this work was to investigate contamination of cereals in Poland with Fusarium and ochratoxin A. Elucidating a correlation between precipitation levels in the individual Provinces and reported levels of the investigated mycotoxins, referring to the generally available meteorological databases, would result in more efficient planning of sampling processes and focusing further preventive actions associated with establishing sampling plans for the following years. Material and methods. Investigations were performed on cereal and cereal product samples taken by the official foodstuffs inspection staff. Some 100 samples were taken annually in the 2009-2012 period (357 samples in total). Tests were performed using high performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS). Precipitation data were obtained from the Central Office of Statistics, based on data received from the Institute of Meteorology and Water Management. Results. Analysis of the influence of precipitation levels during vegetation period on mycotoxin levels in the investigated foodstuffs was performed by associating each recorded content of deoxynivalenol (n=52, corresponding to 14.6% tested samples), zearalenone (n=30, 8.4%), total T-2 and HT-2 toxins (n=21, 5.9%) and ochratoxin A (n=88, 24.6%) above quantification limit with precipitation levels within the Province from which the sample originated. Deoxynivalenol and zearalenone levels show distinct variability corresponding with variability of precipitation levels, well reflecting the reported higher deoxynivalenol and zearalenone levels observed during the rainy years of 2011-2012. Variability in average ochratoxin A levels was not statistically significant. The relatively higher mycotoxin levels in 2009 may result from the heavy rainfall and flooding of 2007-2008. Dependence between the precipitation levels and number of samples showing levels above quantification limit has been also observed for deoxynivalenol. However, a similar analysis made for zearalenone and ochratoxin A does not point to any significant relationship. No data analysis was possible in reference to total T-2 and HT-2 toxins content due to the insufficient number of results available. However, it should be noted that 21% analyzed samples in 2009 contained T-2 and HT-2 levels above the quantification limit, with average of 8.9 μg/kg, whereas in 2010-2012 only one sample of the 263 tested contained contaminants in quantities above the quantification limit. Conclusions. The model used for forecasting presence of mycotoxins in cereals does not allow its practical application during routine generation of official control and monitoring plans on national scale. Notably, tests performed show that exceeding of maximum contamination levels occurred just incidentally, notwithstanding the adverse weather conditions. Further systematic collection of data on mycotoxin contamination of agricultural crops is required for effective continued investigations.
- Published
- 2019
- Full Text
- View/download PDF
22. Exposure assessment of infants and young children on selected Fusarium toxins
- Author
-
Jacek Postupolski, Andrzej Starski, Ewa Ledzion, Jolanta Kurpińska-Jaworska, and Małgorzata Szczęsna
- Subjects
exposure assessment ,mycotoxins ,cereal products ,products for infants and young children ,TDI ,Nutrition. Foods and food supply ,TX341-641 ,Industrial medicine. Industrial hygiene ,RC963-969 ,Public aspects of medicine ,RA1-1270 - Abstract
Background. Mycotoxins belong to substances harmful to human health. They are found mainly in cereal products and their preparations. In particular, infants and young children who consume cereal products, including porridge and gruel, are exposed to these substances. Objective. The aim of the study is to assess the exposure of infants and young children in Poland to micotoxins (ochratoxin A. deoxynivalenol, nivalenol, fumonisins B1 and B1, T-2 and HT-2 toxins) derived from cereal products intended for infants and children. Material and methods. Samples of products (302) were taken from all over the country in the following three years (2011, 2012 and 2013). HPLC-MS / MS method was used to determine the test compounds. Results. Using the HPLC-MS / MS method, the assessment of population exposure in Poland to mikototoxins (ochratoxin A, deoxynivalenol, nivalenol, fumonisins B1 and B1, T-2 and HT-2 toxins) derived from cereal products (porridge, gruel) intended for infants and small children. Samples (302) were taken from across the country over the next three years. The exposure values obtained in the average exposure scenario range from 0.2 to 3% compared to the reference toxicological parameters. Considering that in the case of infants and young children, the tested products constitute a quantitatively significant part of the balanced diet of these consumers, and the remaining groups of foodstuffs, including vegetable products. fruit and meat and dairy products do not contribute significant amounts of mycotoxins to the diet can be accepted. that the level of contamination of cereal products does not pose a significant risk to the health of consumers. In the case of high exposure, it did not exceed 10% of the reference values for deoxynivalenol and the sum of fumonisins B1 and B2. These values were assessed as not relevant for the exposure of infants and young children. In contrast, in the case of zearalenone, the high level of exposure corresponded to 36% of the value of tolerable daily intake (TDI), and for the sum of T-2 and HT-2 toxins, the value of 48% of tolerable daily intake. In both cases, the contribution of pollutants to the diet was significant, but still remained 2-3 times less than the tolerable daily intake. Given, that cereal products are the main source of these contaminants, it can be estimated that exceeding the TDI value in relation to the total diet of infants and young children is unlikely. Conclusions. The exposure values obtained in the average exposure scenario range from 0.2 to 3% compared to the reference toxicological parameters. In the case of zearalenone, the high level of exposure corresponded to 36% of the TDI value. and for the sum of T-2 and HT-2 toxins, 48% TDI. The contribution of pollutants to the diet in both cases was significant. however, it still remained 2-3 times less than the tolerable daily intake. Considering, that cereal products are the main source of these pollutants can be assessed. that exceeding the TDI value for the total diet of infants and young children is unlikely.
- Published
- 2019
- Full Text
- View/download PDF
23. Unique pattern of energy metabolism in endothelial cells from liver sinusoids with oxidative phosphorylation but not glycolysis as a major pathway for ATP generation
- Author
-
Kaczara, Patrycja, Czyzynska-Cichon, Izabela, Kus, Edyta, Kurpinska, Anna, Olkowicz, Mariola, Wojnar-Lason, Kamila, Pacia, Marta Z., Lytvynenko, Olena, Baes, Myriam, and Chlopicki, Stefan
- Published
- 2024
- Full Text
- View/download PDF
24. In Vivo Magnetic Resonance Imaging‐Based Detection of Heterogeneous Endothelial Response in Thoracic and Abdominal Aorta to Short‐Term High‐Fat Diet Ascribed to Differences in Perivascular Adipose Tissue in Mice
- Author
-
Anna Bar, Anna Kieronska‐Rudek, Bartosz Proniewski, Joanna Suraj‐Prażmowska, Krzysztof Czamara, Brygida Marczyk, Karolina Matyjaszczyk‐Gwarda, Agnieszka Jasztal, Edyta Kuś, Zuzanna Majka, Agnieszka Kaczor, Anna Kurpińska, Maria Walczak, Elsbet J. Pieterman, Hans M. G. Princen, and Stefan Chlopicki
- Subjects
endothelial function ,high‐fat diet–fed mice ,magnetic resonance imaging ,perivascular adipose tissue ,thoracic and abdominal aorta ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background Long‐term feeding with a high‐fat diet (HFD) induces endothelial dysfunction in mice, but early HFD‐induced effects on endothelium have not been well characterized. Methods and Results Using an magnetic resonance imaging‐based methodology that allows characterization of endothelial function in vivo, we demonstrated that short‐term (2 weeks) feeding with a HFD to C57BL/6 mice or to E3L.CETP mice resulted in the impairment of acetylcholine‐induced response in the abdominal aorta (AA), whereas, in the thoracic aorta (TA), the acetylcholine‐induced response was largely preserved. Similarly, HFD resulted in arterial stiffness in the AA, but not in the TA. The difference in HFD‐induced response was ascribed to distinct characteristics of perivascular adipose tissue in the TA and AA, related to brown‐ and white‐like adipose tissue, respectively, as assessed by histology, immunohistochemistry, and Raman spectroscopy. In contrast, short‐term HFD‐induced endothelial dysfunction could not be linked to systemic insulin resistance, changes in plasma concentration of nitrite, or concentration of biomarkers of glycocalyx disruption (syndecan‐1 and endocan), endothelial inflammation (soluble form of vascular cell adhesion molecule 1, soluble form of intercellular adhesion molecule 1 and soluble form of E‐selectin), endothelial permeability (soluble form of fms‐like tyrosine kinase 1 and angiopoietin 2), and hemostasis (tissue plasminogen activator and plasminogen activator inhibitor 1). Conclusions Short‐term feeding with a HFD induces endothelial dysfunction in the AA but not in the TA, which could be ascribed to a differential response of perivascular adipose tissue to a HFD in the AA versus TA. Importantly, early endothelial dysfunction in the AA is not linked to elevation of classical systemic biomarkers of endothelial dysfunction.
- Published
- 2020
- Full Text
- View/download PDF
25. Application of Artificial Neural Networks to Predict Insulation Properties of Lightweight Concrete
- Author
-
Marzena Kurpińska, Leszek Kułak, Tadeusz Miruszewski, and Marcin Byczuk
- Subjects
artificial neural networks ,thermal conductivity ,lightweight concrete ,lightweight aggregate ,predicting properties ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Predicting the properties of concrete before its design and application process allows for refining and optimizing its composition. However, the properties of lightweight concrete are much harder to predict than those of normal weight concrete, especially if the forecast concerns the insulating properties of concrete with artificial lightweight aggregate (LWA). It is possible to use porous aggregates and precisely modify the composition of lightweight concrete (LWC) with specific insulating properties. In this case, it is advisable to determine the parameters of the components and perform preliminary laboratory tests, and then use theoretical methods (e.g., artificial neural networks (ANNs) to predict not only the mechanical properties of lightweight concrete, but also its thermal insulation properties. Fifteen types of lightweight concrete, differing in light filler, were tested. Lightweight aggregates with different grain diameters and lightweight aggregate grains with different porosity were used. For the tests, expanded glass was applied as a filler with very good thermal insulation properties and granulated sintered fly ash, characterized by a relatively low density and high crushing strength in the group of LWAs. The aim of the work is to demonstrate the usefulness of an ANN for the determination of the relationship between the selection of the type and quantity of LWA and porosity, density, compressive strength, and thermal conductivity (TC) of the LWC.
- Published
- 2021
- Full Text
- View/download PDF
26. COVID-19-related social isolation and symptoms of depression and anxiety in young men in Poland: Does insomnia mediate the relationship?
- Author
-
Mojsa-Kaja, Justyna, primary, Szklarczyk-Smolana, Klaudia, additional, Niedzielska-Andres, Ewa, additional, Kurpińska, Anna, additional, Suraj-Prażmowska, Joanna, additional, and Walczak, Maria, additional
- Published
- 2023
- Full Text
- View/download PDF
27. Early and late endothelial response in breast cancer metastasis in mice: simultaneous quantification of endothelial biomarkers using a mass spectrometry-based method
- Author
-
Joanna Suraj, Anna Kurpińska, Agnieszka Zakrzewska, Magdalena Sternak, Marta Stojak, Agnieszka Jasztal, Maria Walczak, and Stefan Chlopicki
- Subjects
Endothelium ,Murine 4T1 model of breast cancer ,Metastasis ,Biomarkers ,MicroLC/MS-MRM ,Medicine ,Pathology ,RB1-214 - Abstract
The endothelium plays an important role in cancer metastasis, but the mechanisms involved are still not clear. In the present work, we characterised the changes in endothelial function at early and late stages of breast cancer progression in an orthotopic model of murine mammary carcinoma (4T1 cells). Endothelial function was analysed based on simultaneous microflow liquid chromatography–tandem mass spectrometry using multiple reaction monitoring (microLC/MS-MRM) quantification of 12 endothelium-related biomarkers, including those reflecting glycocalyx disruption – syndecan-1 (SDC-1), endocan (ESM-1); endothelial inflammation – vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1), E-selectin (E-sel); endothelial permeability – fms-like tyrosine kinase 1 (FLT-1), angiopoietin 2 (Angpt-2); and haemostasis – von Willebrand factor (vWF), tissue plasminogen activator (t-PA), plasminogen activator inhibitor 1 (PAI-1), as well as those that are pathophysiologically linked to endothelial function – adrenomedullin (ADM) and adiponectin (ADN). The early phase of metastasis in mouse plasma was associated with glycocalyx disruption (increased SDC-1 and ESM-1), endothelial inflammation [increased soluble VCAM-1 (sVCAM-1)] and increased vascular permeability (Angpt-2). During the late phase of metastasis, additional alterations in haemostasis (increased PAI-1 and vWF), as well as a rise in ADM and substantial fall in ADN concentration, were observed. In conclusion, in a murine model of breast cancer metastasis, we identified glycocalyx disruption, endothelial inflammation and increased endothelial permeability as important events in early metastasis, while the late phase of metastasis was additionally characterised by alterations in haemostasis.
- Published
- 2019
- Full Text
- View/download PDF
28. Degradation of Glycocalyx and Multiple Manifestations of Endothelial Dysfunction Coincide in the Early Phase of Endothelial Dysfunction Before Atherosclerotic Plaque Development in Apolipoprotein E/Low‐Density Lipoprotein Receptor‐Deficient Mice
- Author
-
Anna Bar, Marta Targosz‐Korecka, Joanna Suraj, Bartosz Proniewski, Agnieszka Jasztal, Brygida Marczyk, Magdalena Sternak, Magdalena Przybyło, Anna Kurpińska, Maria Walczak, Renata B. Kostogrys, Marek Szymonski, and Stefan Chlopicki
- Subjects
atherosclerosis ,atomic force microscopy ,endothelial function ,glycocalyx ,magnetic resonance imaging ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background The impairment of endothelium‐dependent vasodilation, increased endothelial permeability, and glycocalyx degradation are all important pathophysiological components of endothelial dysfunction. However, it is still not clear whether in atherosclerosis, glycocalyx injury precedes other features of endothelial dysfunction or these events coincide. Methods and Results Herein, we demonstrate that in 4‐ to 8‐week‐old apolipoprotein E/low‐density lipoprotein receptor‐deficient mice, at the stage before development of atherosclerotic plaques, impaired acetylcholine‐induced vasodilation, reduced NO production in aorta, and increased endothelial permeability were all observed; however, flow‐mediated dilation in the femoral artery was fully preserved. In 4‐week‐old mice, glycocalyx coverage was reduced and endothelial stiffness was increased, whereas glycocalyx length was significantly decreased at 8 weeks of age. Early changes in endothelial function were also featured by increased plasma concentration of biomarkers of glycocalyx disruption (endocan), biomarkers of endothelial inflammation (soluble vascular cell adhesion molecule 1), increased vascular permeability (angiopoietin 2), and alterations in hemostasis (tissue plasminogen activator and plasminogen activator inhibitor 1). In 28‐week‐old mice, at the stage of advanced atherosclerotic plaque development, impaired NO production and nearly all other features of endothelial dysfunction were changed to a similar extent, compared with the preatherosclerotic plaque phase. The exceptions were the occurrence of acetylcholine‐induced vasoconstriction in the aorta and brachiocephalic artery, impaired flow‐mediated vasodilation in the femoral artery, and further reduction of glycocalyx length and coverage with a concomitant further increase in endothelial permeability. Conclusions In conclusion, even at the early stage before the development of atherosclerotic plaques, endothelial dysfunction is a complex multifactorial response that has not been previously appreciated.
- Published
- 2019
- Full Text
- View/download PDF
29. The impact of natural fibers’ characteristics on mechanical properties of the cement composites
- Author
-
Kurpińska, Marzena, primary, Pawelska-Mazur, Magdalena, additional, Gu, Yining, additional, and Kurpiński, Filip, additional
- Published
- 2022
- Full Text
- View/download PDF
30. Development, validation and application of a micro–liquid chromatography–tandem mass spectrometry based method for simultaneous quantification of selected protein biomarkers of endothelial dysfunction in murine plasma
- Author
-
Suraj, Joanna, Kurpińska, Anna, Olkowicz, Mariola, Niedzielska–Andres, Ewa, Smolik, Magdalena, Zakrzewska, Agnieszka, Jasztal, Agnieszka, Sitek, Barbara, Chlopicki, Stefan, and Walczak, Maria
- Published
- 2018
- Full Text
- View/download PDF
31. Badanie kompozytów cementowych ze składnikiem pochodzącym z recyklingu łopat turbin wiatrowych.
- Author
-
JEDLECKI, MIKOŁAJ, ZIÓŁKOWSKA, OLIWIA, KURPIŃSKA, MARZENA, KONIECKO, KRZESIMIR, and SZULIŃSKA, AGATA
- Abstract
Copyright of Builder (1896-0642) is the property of PWB MEDIA Zdzieblowski sp.j. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
- Full Text
- View/download PDF
32. Influence of the Addition of Recycled Aggregates and Polymer Fibers on the Properties of Pervious Concrete.
- Author
-
Mitrosz, Oskar, Kurpińska, Marzena, Miśkiewicz, Mikołaj, Brzozowski, Tadeusz, and Abdelgader, Hakim Salem
- Subjects
- *
POLYMER aggregates , *RECYCLED concrete aggregates , *RUBBER waste , *CONCRETE waste , *WASTE recycling , *LIGHTWEIGHT concrete , *WASTE products as building materials - Abstract
The aim of the study was to check the possibility of reusing aggregate from recycled concrete waste and rubber granules from car tires as partial substitution of natural aggregate. The main objective was to investigate the effects of recycled waste aggregate modified with polymer fibers on the compressive and flexural strength, modulus of elasticity and permeability of pervious concrete. Fibers with a multifilament structure and length of 54 mm were deliberately used to strengthen the joints among grains (max size 31.5 mm). Eight batches of designed mixes were used in the production of pervious concrete at fixed water/binder ratio of 0.34 with cement content of 350 kg/m3. Results showed that the use of recycled concrete aggregate (8/31.5 mm) with replacement ratio of 50% (by weight of aggregate) improved the mechanical properties of pervious concrete in all analyzed cases. Whereas the replacement of 10% rubber waste aggregate (2/5 mm) by volume of aggregate reduced the compressive strength by a maximum of 11.4%. Addition of 2 kg/m3 of polymer fibers proved the strengthening effect of concrete structure, enhancing the compressive and tensile strengths by a maximum of 23.4% and 25.0%, respectively. The obtained test results demonstrate the possibility of using the recycled waste aggregates in decarbonization process of pervious concrete production, but further laboratory and field performance tests are needed. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
33. Comparison of anti-cancer effects of novel protein disulphide isomerase (PDI) inhibitors in breast cancer cells characterized by high and low PDIA17 expression
- Author
-
Kurpińska, Anna, primary, Suraj-Prażmowska, Joanna, additional, Stojak, Marta, additional, Jarosz, Joanna, additional, Mateuszuk, Łukasz, additional, Niedzielska-Andres, Ewa, additional, Smolik, Magdalena, additional, Wietrzyk, Joanna, additional, Kalvins, Ivars, additional, Walczak, Maria, additional, and Chłopicki, Stefan, additional
- Published
- 2022
- Full Text
- View/download PDF
34. Hormonal changes in dairy cows during periparturient period
- Author
-
Wiesław Skrzypczak and Anna Kurpińska
- Subjects
Animal science ,business.industry ,Period (gene) ,General Earth and Planetary Sciences ,Medicine ,business ,General Environmental Science ,Hormone - Published
- 2020
- Full Text
- View/download PDF
35. Maturity curve for estimating the in-place strength of high performance concrete
- Author
-
Mariak Aleksandra, Kurpińska Marzena, and Wilde Krzysztof
- Subjects
Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The paper presents the maturity curve for estimating the in-place early-age compressive strength of concrete. The development of appropriate maturity curve is a complex process. It is important to correctly determine the datum temperature and activation energy, which can be obtained in mortar tests. This paper describes an investigation of the accuracy of the maturity method to estimate the strength when different way to rate constant and maturity function are analysed. High performance concrete used for construction of extradosed bridge was tested. The developed curve was implemented to predict the concrete strength at three construction stages, in summer, autumn and early spring.
- Published
- 2019
- Full Text
- View/download PDF
36. 10 - Cement kiln dust
- Author
-
Abdelgader, Hakim S., Amran, Mugahed, Kurpińska, Marzena, Mosaberpanah, Mohammad A., Murali, Gunasekaran, and Fediuk, Roman
- Published
- 2022
- Full Text
- View/download PDF
37. Additional file 1 of Comparison of anti-cancer effects of novel protein disulphide isomerase (PDI) inhibitors in breast cancer cells characterized by high and low PDIA17 expression
- Author
-
Kurpińska, Anna, Suraj-Prażmowska, Joanna, Stojak, Marta, Jarosz, Joanna, Mateuszuk, Łukasz, Niedzielska-Andres, Ewa, Smolik, Magdalena, Wietrzyk, Joanna, Kalvins, Ivars, Walczak, Maria, and Chłopicki, Stefan
- Abstract
Additional file 1: Methods. Contains all additional information on methods used in the study.
- Published
- 2022
- Full Text
- View/download PDF
38. Mix Design and Mechanical Properties of Pervious Concrete Containing Recycled Concrete and Rubber Waste Aggregate Modified with Polymer Fibers
- Author
-
Oskar Mitrosz, Marzena Kurpińska, Mikołaj Miśkiewicz, and Tadeusz Brzozowski
- Published
- 2022
- Full Text
- View/download PDF
39. Cement kiln dust
- Author
-
Hakim S. Abdelgader, Mugahed Amran, Marzena Kurpińska, Mohammad A. Mosaberpanah, Gunasekaran Murali, and Roman Fediuk
- Published
- 2022
- Full Text
- View/download PDF
40. Contributors
- Author
-
Hakim S. Abdelgader, Farshad Ameri, Mugahed Amran, Beyza Fahriye Aygün, Regina Mambeli Barros, Turhan Bilir, Chee Ban Cheah, Loredana Contrafatto, Ramazan Demirboga, Weiwei Duan, Khatib Zada Farhan, Roman Fediuk, Romildo Dias Toledo Filho, Cintia Maria Ariani Fontes, Nikita Gupta, Zahid Hossain, Kazi Tamzidul Islam, Ömer Karadağ, Mehrnoush Khavarian, Hyeong-Ki Kim, Marzena Kurpińska, Jay Sern Lim, Yue Liu, Mohammad A. Mosaberpanah, Gunasekaran Murali, Rafat Siddique, Jagdeep Singh, Malkit Singh, Waiching Tang, Ali Yousefi, and Yan Zhuge
- Published
- 2022
- Full Text
- View/download PDF
41. Protein disulfide isomerase-A1 regulates intraplatelet reactive oxygen speciesthromboxane A2-dependent pathway in human platelets
- Author
-
Joanna Suraj-Prazmowska, Kamil Karolczak, Stefan Chlopicki, Patrycja Kaczara, Agnieszka Kij, Anna Kurpińska, Agata Malinowska, Cezary Watala, Dagmara Wojkowska, Ivars Kalvins, Kamil Przyborowski, and Agnieszka Pelesz
- Subjects
Thromboxane ,flow cytometry ,Convulxin ,Hematology ,PDIA3 ,Cell biology ,Thromboxane A2 ,chemistry.chemical_compound ,Thrombin ,proteomics ,chemistry ,bepristat ,PDIs expression ,medicine ,platelet activation ,Platelet ,Platelet activation ,Protein disulfide-isomerase ,medicine.drug - Abstract
Background Platelet-derived protein disulfide isomerase 1 (PDIA1) regulates thrombus formation, but its role in the regulation of platelet function is not fully understood. Aims The aim of this study was to characterize the role of PDIA1 in human platelets. Methods Proteomic analysis of PDI isoforms in platelets was performed using LC-MS/MS, and the expression of PDIs on platelets in response to collagen, TRAP-14, or ADP was measured with flow cytometry. The effects of bepristat, a selective PDIA1 inhibitor, on platelet aggregation, expression of platelet surface activation markers, thromboxane A2 (TxA2 ) and reactive oxygen species (ROS) generation were evaluated by optical aggregometry, flow cytometry, enzyme-linked immunosorbent assay and dihydrodichlorofluorescein diacetate-based fluorescent assay, respectively. Results PDIA1 was less abundant compared to PDIA3 in resting platelets and platelets stimulated with TRAP-14, collagen or ADP. Collagen, but not ADP, induced a significant increase in PDIA1 expression. Bepristat potently inhibited the aggregation of washed platelets induced by collagen or convulxin, but only weakly inhibited platelet aggregation induced by TRAP-14 or thrombin, and had the negligible effect on platelet aggregation induced by arachidonic acid. Inhibition of PDIA1 by bepristat resulted in the reduction of thromboxane A2 and ROS production in collagen- or thrombin-stimulated platelets. Furthermore, bepristat reduced the activation of αIIbβ3 integrin and expression of P-selectin. Conclusions PDIA1 acts as an intraplatelet regulator of the ROS-TxA2 pathway in collagen-GP VI receptor-mediated platelet activation that is a mechanistically distinct pathway from extracellular regulation of αIIbβ3 integrin by PDIA3.
- Published
- 2022
42. Additional file 2 of Comparison of anti-cancer effects of novel protein disulphide isomerase (PDI) inhibitors in breast cancer cells characterized by high and low PDIA17 expression
- Author
-
Kurpińska, Anna, Suraj-Prażmowska, Joanna, Stojak, Marta, Jarosz, Joanna, Mateuszuk, Łukasz, Niedzielska-Andres, Ewa, Smolik, Magdalena, Wietrzyk, Joanna, Kalvins, Ivars, Walczak, Maria, and Chłopicki, Stefan
- Abstract
Additional file 2: Table S1. The origin and conditions of maintenance of cell cultures.
- Published
- 2022
- Full Text
- View/download PDF
43. Additional file 3 of Comparison of anti-cancer effects of novel protein disulphide isomerase (PDI) inhibitors in breast cancer cells characterized by high and low PDIA17 expression
- Author
-
Kurpińska, Anna, Suraj-Prażmowska, Joanna, Stojak, Marta, Jarosz, Joanna, Mateuszuk, Łukasz, Niedzielska-Andres, Ewa, Smolik, Magdalena, Wietrzyk, Joanna, Kalvins, Ivars, Walczak, Maria, and Chłopicki, Stefan
- Abstract
Additional file 3: Table S2. The half maximal inhibitory concentration for selected PDIA1, PDIA3 and PDIA17 inhibitors.
- Published
- 2022
- Full Text
- View/download PDF
44. Mix Design and Mechanical Properties of Pervious Concrete Containing Recycled Concrete and Rubber Waste Aggregate Modified with Polymer Fibers
- Author
-
Mitrosz, Oskar, primary, Kurpińska, Marzena, additional, Miśkiewicz, Mikołaj, additional, and Brzozowski, Tadeusz, additional
- Published
- 2022
- Full Text
- View/download PDF
45. Application of Artificial Neural Networks to Predict Insulation Properties of Lightweight Concrete
- Author
-
Kurpińska, Marzena, primary, Kułak, Leszek, additional, Miruszewski, Tadeusz, additional, and Byczuk, Marcin, additional
- Published
- 2021
- Full Text
- View/download PDF
46. Parameters of protein and iron metabolism in dairy cows during periparturient period
- Author
-
Anna Kurpińska, Wiesław Skrzypczak, and A. Jarosz
- Subjects
Creatinine ,Pregnancy ,Period (gene) ,Protein metabolism ,Biology ,medicine.disease ,chemistry.chemical_compound ,Animal science ,Blood chemistry ,chemistry ,medicine ,General Earth and Planetary Sciences ,Nutrition physiology ,Dairy cattle ,Postpartum period ,General Environmental Science - Published
- 2020
- Full Text
- View/download PDF
47. The effect of macro polymer fibres length and content on the fibre reinforced concrete
- Author
-
Mariak Aleksandra and Kurpińska Marzena
- Subjects
Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
The paper presents studies of a ready-mix concrete containing polymer fibres of three different lengths: 24, 38 and 54 mm. The performed tests allowed to determine the effect of fibre volume fraction and length on the concrete strength. The basic parameters of concrete mixture (consistency, air content and bulk density) were identified. Fibre reinforced concrete belongs to a group of composite materials. The polymer fibres are applied in the concrete in structures where the reduction of shrinkage cracking as well as corrosion resistance and fire temperatures are required. It is widely known, that the cracking behaviour of concrete structures depends on flexural tensile strength of concrete. The addition of fibres significantly improves the tensile strength. The experimental study, including axial compressive strength and center-point loading flexural tensile strength, was carried out. The scope of the research was also expanded by the usage of a scanning microscope. The test results showed the effect of fibre length and fibre combinations on mechanical properties of concrete. The effect of the research is to formulate guidelines due to the quantity of macro polymer fibres. In general, appropriate fibre content brings a beneficial effect e.g. improves better workability of a concrete mixture.
- Published
- 2018
- Full Text
- View/download PDF
48. Assessment of changes in the occurrence of Fusarium toxin and ochratoxin A in Poland related to extreme weather phenomena
- Author
-
M. Szczesna, Ewa Ledzion, Jolanta Kurpińska-Jaworska, Jacek Postupolski, and Andrzej Starski
- Subjects
Crops, Agricultural ,Fusarium ,Ochratoxin A ,Fusarium toxin ,040301 veterinary sciences ,Food Contamination ,lcsh:TX341-641 ,010501 environmental sciences ,cereal products ,01 natural sciences ,0403 veterinary science ,Toxicology ,chemistry.chemical_compound ,lcsh:RC963-969 ,weather condition ,mycotoxins ,Humans ,Extreme Weather ,Organic matter ,Mycotoxin ,Ochratoxin ,Zearalenone ,Chromatography, High Pressure Liquid ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Aspergillus ,biology ,lcsh:Public aspects of medicine ,formation ,food and beverages ,lcsh:RA1-1270 ,04 agricultural and veterinary sciences ,General Medicine ,prediction ,Contamination ,biology.organism_classification ,Ochratoxins ,chemistry ,lcsh:Industrial medicine. Industrial hygiene ,Environmental science ,Poland ,Edible Grain ,ochratoxin A ,lcsh:Nutrition. Foods and food supply ,Food Analysis - Abstract
Background. Mycotoxins – substances harmful to humans, are ubiquitous in the environment. Mycotoxins are generated primarily by Penicilium, Aspergillus and Fusarium genus fungi. Their presence is associated with the unavoidable presence of mold fungi in the environment. The presently observed adverse climatic changes could negatively affect agriculture, causing erosion and loss of organic matter from soil, promulgation of pests and plant diseases, including those originating from pathogenic molds, and also migration of certain mold species into new regions, ultimately creating more favorable conditions for generation of mycotoxins. Objective. The purpose of this work was to investigate contamination of cereals in Poland with Fusarium and ochratoxin A. Elucidating a correlation between precipitation levels in the individual Provinces and reported levels of the investigated mycotoxins, referring to the generally available meteorological databases, would result in more efficient planning of sampling processes and focusing further preventive actions associated with establishing sampling plans for the following years. Material and methods. Investigations were performed on cereal and cereal product samples taken by the official foodstuffs inspection staff. Some 100 samples were taken annually in the 2009-2012 period (357 samples in total). Tests were performed using high performance liquid chromatography coupled with mass spectrometry (HPLC-MS/MS). Precipitation data were obtained from the Central Office of Statistics, based on data received from the Institute of Meteorology and Water Management. Results. Analysis of the influence of precipitation levels during vegetation period on mycotoxin levels in the investigated foodstuffs was performed by associating each recorded content of deoxynivalenol (n=52, corresponding to 14.6% tested samples), zearalenone (n=30, 8.4%), total T-2 and HT-2 toxins (n=21, 5.9%) and ochratoxin A (n=88, 24.6%) above quantification limit with precipitation levels within the Province from which the sample originated. Deoxynivalenol and zearalenone levels show distinct variability corresponding with variability of precipitation levels, well reflecting the reported higher deoxynivalenol and zearalenone levels observed during the rainy years of 2011-2012. Variability in average ochratoxin A levels was not statistically significant. The relatively higher mycotoxin levels in 2009 may result from the heavy rainfall and flooding of 2007-2008. Dependence between the precipitation levels and number of samples showing levels above quantification limit has been also observed for deoxynivalenol. However, a similar analysis made for zearalenone and ochratoxin A does not point to any significant relationship. No data analysis was possible in reference to total T-2 and HT-2 toxins content due to the insufficient number of results available. However, it should be noted that 21% analyzed samples in 2009 contained T-2 and HT-2 levels above the quantification limit, with average of 8.9 μg/kg, whereas in 2010-2012 only one sample of the 263 tested contained contaminants in quantities above the quantification limit. Conclusions. The model used for forecasting presence of mycotoxins in cereals does not allow its practical application during routine generation of official control and monitoring plans on national scale. Notably, tests performed show that exceeding of maximum contamination levels occurred just incidentally, notwithstanding the adverse weather conditions. Further systematic collection of data on mycotoxin contamination of agricultural crops is required for effective continued investigations.
- Published
- 2019
49. Exposure assessment of infants and young children on selected Fusarium toxins
- Author
-
M. Szczesna, Jolanta Kurpińska-Jaworska, Jacek Postupolski, Andrzej Starski, and Ewa Ledzion
- Subjects
Tolerable daily intake ,Ochratoxin A ,exposure assessment ,products for infants and young children ,Food Contamination ,lcsh:TX341-641 ,cereal products ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,lcsh:RC963-969 ,Fusarium ,TDI ,mycotoxins ,Humans ,Fusarium toxins ,Medicine ,Child ,Mycotoxin ,Ochratoxin ,Zearalenone ,030304 developmental biology ,Exposure assessment ,0303 health sciences ,business.industry ,lcsh:Public aspects of medicine ,030302 biochemistry & molecular biology ,Infant ,food and beverages ,lcsh:RA1-1270 ,Environmental Exposure ,General Medicine ,Human nutrition ,chemistry ,Child, Preschool ,lcsh:Industrial medicine. Industrial hygiene ,Poland ,Edible Grain ,business ,lcsh:Nutrition. Foods and food supply - Abstract
Background. Mycotoxins belong to substances harmful to human health. They are found mainly in cereal products and their preparations. In particular, infants and young children who consume cereal products, including porridge and gruel, are exposed to these substances. Objective. The aim of the study is to assess the exposure of infants and young children in Poland to micotoxins (ochratoxin A. deoxynivalenol, nivalenol, fumonisins B1 and B1, T-2 and HT-2 toxins) derived from cereal products intended for infants and children. Material and methods. Samples of products (302) were taken from all over the country in the following three years (2011, 2012 and 2013). HPLC-MS / MS method was used to determine the test compounds. Results. Using the HPLC-MS / MS method, the assessment of population exposure in Poland to mikototoxins (ochratoxin A, deoxynivalenol, nivalenol, fumonisins B1 and B1, T-2 and HT-2 toxins) derived from cereal products (porridge, gruel) intended for infants and small children. Samples (302) were taken from across the country over the next three years. The exposure values obtained in the average exposure scenario range from 0.2 to 3% compared to the reference toxicological parameters. Considering that in the case of infants and young children, the tested products constitute a quantitatively significant part of the balanced diet of these consumers, and the remaining groups of foodstuffs, including vegetable products. fruit and meat and dairy products do not contribute significant amounts of mycotoxins to the diet can be accepted. that the level of contamination of cereal products does not pose a significant risk to the health of consumers. In the case of high exposure, it did not exceed 10% of the reference values for deoxynivalenol and the sum of fumonisins B1 and B2. These values were assessed as not relevant for the exposure of infants and young children. In contrast, in the case of zearalenone, the high level of exposure corresponded to 36% of the value of tolerable daily intake (TDI), and for the sum of T-2 and HT-2 toxins, the value of 48% of tolerable daily intake. In both cases, the contribution of pollutants to the diet was significant, but still remained 2-3 times less than the tolerable daily intake. Given, that cereal products are the main source of these contaminants, it can be estimated that exceeding the TDI value in relation to the total diet of infants and young children is unlikely. Conclusions. The exposure values obtained in the average exposure scenario range from 0.2 to 3% compared to the reference toxicological parameters. In the case of zearalenone, the high level of exposure corresponded to 36% of the TDI value. and for the sum of T-2 and HT-2 toxins, 48% TDI. The contribution of pollutants to the diet in both cases was significant. however, it still remained 2-3 times less than the tolerable daily intake. Considering, that cereal products are the main source of these pollutants can be assessed. that exceeding the TDI value for the total diet of infants and young children is unlikely.
- Published
- 2019
50. Experimental Study of the Resistance to Influence of Aggressive Liquids on Lightweight Concrete
- Author
-
Marzena Kurpińska and Elżbieta Haustein
- Subjects
Technology ,Materials science ,0211 other engineering and technologies ,02 engineering and technology ,Article ,Corrosion ,chemistry.chemical_compound ,granulated ash aggregate ,021105 building & construction ,aggressive liquids ,General Materials Science ,Composite material ,Microscopy ,QC120-168.85 ,Aggregate (composite) ,QH201-278.5 ,Penetration (firestop) ,lightweight concrete ,Engineering (General). Civil engineering (General) ,021001 nanoscience & nanotechnology ,Microstructure ,compressive strength ,Durability ,TK1-9971 ,Phenolphthalein ,Compressive strength ,fly ash ,Descriptive and experimental mechanics ,chemistry ,Fly ash ,Electrical engineering. Electronics. Nuclear engineering ,TA1-2040 ,0210 nano-technology ,absorption - Abstract
In light of the scientific research, the corrosion of concrete structures is one of the main problems that may reduce their durability due to the negative impact of the natural environment. The paper analyzes the influence of the type of component on the selected properties of lightweight concrete subjected to the influence of aggressive liquids. Four concrete mixes were prepared with a granular aggregate made of foamed glass (GEGA) and aggregate made of sintered fly ash (GAA) with the use of a mineral additive: silica fly ash. The prepared lightweight concrete after one year was exposed for 60 days to the following environments: strong acid—HCl, 1% and 2% concentration, weak acid—CH3COOH, 1% and 2% concentration, and an aqueous salt solution of Na2SO4, 1% and 2% concentration. Then, the compressive strength was tested, and the microstructure analysis of the ready-made lightweight concrete (LWC) was performed. The degree of penetration of aggressive solutions into the cracks of the samples was assessed by means of applying 1% phenolphthalein solution. Changes in the weight of lightweight concrete samples after the test period were estimated. The obtained test results indicate that the decrease in the durability of lightweight concrete can be classified as a long-term process. Concrete with GEGA and GAA showed high resistance to aggressive environments. Moreover, the environment containing chlorides turned out to be the most aggressive, while the environment containing sulfates proved to be the least aggressive. The higher the concentration of the destructive factor was, the faster the corrosion process went. This has been proven by measuring the pH using phenolphthalein and carrying out microscopic examination. Concretes containing aggregates made of foamed glass and sintered fly ash are suitable for use both in traditional construction and in facilities exposed to an aggressive environment (e.g., in the chemical industry and at gas stations).
- Published
- 2021
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.