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Your search keyword '"Kostrz, Dorota"' showing total 11 results
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11 results on '"Kostrz, Dorota"'

3. Modulation of SARS-CoV-2 spike binding to ACE2 through conformational selection

Author

Saha, Prithwidip, primary, Fernanadez, Ignacio, additional, Sumbul, Fidan, additional, Valotteau, Claire, additional, Kostrz, Dorota, additional, Meola, Annalisa, additional, Baquero, Eduard, additional, Sharma, Arvind, additional, Portman, James R., additional, Stransky, Francois, additional, Boudier, Thomas, additional, Guardado Calvo, Pablo, additional, Gosse, Charlie, additional, Strick, Terence, additional, Rey, Felix A., additional, and Rico, Felix, additional

Published
2024
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7. Combining DNA scaffolds and acoustic force spectroscopy to characterize individual protein bonds

Author

Wang, Yong, Valotteau, Claire, Aimard, Adrien, Villanueva, Lorenzo, Kostrz, Dorota, Follenfant, Maryne, Strick, Terence, Chames, Patrick, Rico, Felix, Gosse, Charlie, Limozin, Laurent, Adhésion et Inflammation (LAI), Aix Marseille Université (AMU)-Assistance Publique - Hôpitaux de Marseille (APHM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie de l'ENS Paris (IBENS), Département de Biologie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), AMIDEX Emergence Innovation, and Plan Cancer PhysCancer

Subjects
[PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det]
Abstract

International audience; Single-molecule data are of great significance in biology, chemistry, and medicine. However, new experimental tools to characterize, in a multiplexed manner, protein bond rupture under force are still needed. Acoustic force spectroscopy (AFS) is an emerging manipulation technique which generates acoustic waves to apply force in parallel on multiple microbeads tethered to a surface. We here exploite this configuration in combination with the recently developed modular junctured-DNA (J-DNA) scaffold that have been designed to study protein-protein interactions at the single-molecule level. By applying repetitive constant force steps on the FKBP12-rapamycin-FRB complex, we measure its unbinding kinetics under force at the single-bond level. Special efforts are made in analyzing the data in order to identify potential pitfalls. We propose a calibration method allowing in situ force determination during the course of the unbinding measurement. We compare our results with well-established techniques, such as magnetic tweezers, to ensure their accuracy. We also apply our strategy to study the force dependent rupture of a single domain antibody with its antigen. Overall, we get a good agreement with the published parameters that have been obtained at zero force and population level. Thus, our technique offers single-molecule precision for multiplexed measurements of interactions of biotechnological and medical interest.

Published
2022
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9. Combining Acoustic Force Spectroscopy and DNA Scaffold for High Throughput Measurement of Ligand-Receptor Kinetics at Single Molecule Resolution

Author

Jian Wang, Yong, primary, Valotteau, Claire, additional, Aimard, Adrien, additional, Villanueva, Lorenzo, additional, Kostrz, Dorota, additional, Follenfant, Maryne, additional, Strick, Terence, additional, Gosse, Charlie, additional, Chames, Patrick, additional, Rico, Felix, additional, and Limozin, Laurent, additional

Published
2021
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