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Enterotoxigenic Escherichia coli (ETEC)-mediated diarrhea can be mitigated by inhibiting bacterial adhesion to intestinal surface. Some lactic acid bacteria (LAB) produce exopolysaccharides (EPS) that can inhibit ETEC adhesion. In this study, we fermented soy flour-based dough (SoyD) with EPS-producing LAB strains Pediococcus pentosaceus TL (PpTL), Leuconostoc citreum TR (LcTR), Leuconostoc mesenteroides WA (LmWA) and L. mesenteroides WN (LmWN) to improve anti-adhesive activity of the dough against ETEC. The strains LcTR, LmWA and LmWN produced EPS in SoyD fermentation with similar polysaccharide yields and compositions as when grown in liquid medium, whereas PpTL was unable to produce EPS in SoyD. LcTR produced high molecular weight (Mw) dextran (∼900 kDa) while LmWA and LmWN produced dextran and levan with diverse Mw (∼20-1000 kDa). SoyD fermentation by EPS-producing LAB increased the capability of the SoyD extracts to adhere to ETEC cells and block ETEC adhesion to porcine mucin. After Mw-based fractionation, all extract-fractions (>3 kDA) of LmWA- and LmWN-fermented SoyD retained their blocking activity indicating that various Mw populations of the EPS contributes to bioactivity against ETEC. This study shows the potential of EPS-producing LAB strains as fermenting microorganisms in the development of a functional food product with anti-diarrheal properties., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Considering the positive health effects of leisure-time physical activity (LTPA), youth is an important life stage to promote lifelong LTPA. However, the stability of LTPA over the life course is low, and specific predictors of LTPA in youth for lifelong activity have some shortcomings, e.g. neglecting the interacting factors of LTPA within individuals. Therefore, from a person-oriented approach, patterns of LTPA behaviour in youth considering time- and context-related aspects and their relationships with lifelong LTPA were investigated. Life course data from n  = 1519 Swiss inhabitants aged between 25 and 76 years were recorded retrospectively using a validated questionnaire (CATI method). Latent profile analyses were used to find the optimal profile solution and for the association with lifelong LTPA auxiliary conditional effect models (controlled for age) were applied. Six distinct patterns emerged. Overall, mostly inactive youth are also the least active in adulthood, whereas several other patterns are associated with a mainly continuous LTPA throughout adulthood. More precisely, multiple constellations in youth occurred to be physically active in at least 80% of the years in adulthood: (1) early starters regarding LTPA in a rather self-organised setting but not with many different LTPAs; (2) late entrants with a variety of different activities and organisational settings; or (3) a high expression in every variable investigated. Consequently, there is not just one type of LTPA behaviour in youth linked to lifelong activity, which indicates that certain aspects of LTPA in youth can be compensated by each other. Implications for LTPA promotion can be derived., Supplementary Information: The online version of this article (10.1007/s12662-023-00884-9) contains supplementary material, which is available to authorized users., Competing Interests: Conflict of interestL. Lenze, C. Klostermann, J. Schmid, M. Lamprecht and S. Nagel declare that they have no competing interests., (© The Author(s) 2023.)

Resistant starch (RS) results in relatively high health-beneficial butyrate levels upon fermentation by gut microbiota. We studied how physico-chemical characteristics of RS-3 influenced butyrate production during fermentation. Six highly resistant RS-3 substrates (intrinsic RS-3, 80-95 % RS) differing in chain length (DPn 16-76), Mw distribution (PI) and crystal type (A/B) were fermented in vitro by pooled adult faecal inoculum. All intrinsic RS-3 substrates were fermented to relatively high butyrate levels (acetate/butyrate ≤ 2.5), and especially fermentation of A-type RS-3 prepared from polydisperse α-1,4 glucans resulted in the highest relative butyrate amount produced (acetate/butyrate: 1). Analysis of the microbiota composition after fermentation revealed that intrinsic RS-3 stimulated primarily Lachnospiraceae, Bifidobacterium and Ruminococcus, but the relative abundances of these taxa differed slightly depending on the RS-3 physico-chemical characteristics. Especially intrinsic RS-3 of narrow disperse Mw distribution stimulated relatively more Ruminococcus. Selected RS fractions (polydisperse Mw distribution) obtained after pre-digestion were fermented to acetate and butyrate (ratio ≤ 1.8) and stimulated Lachnospiraceae and Bifidobacterium. This study indicates that especially the α-1,4 glucan Mw distribution dependent microstructure of RS-3 influences butyrate production and microbiota composition during RS-3 fermentation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)

This case report describes the successful fixation of a periprosthetic patellar fracture in an 89-year-old female patient after total knee arthroplasty (TKA) and cemented retropatellar component. Fixation was performed by use of a fixed-angle plate combined with cerclage, under preservation of the firmly fixed retropatellar prosthetic component. No complications were observed postoperatively. Radiologic controls confirmed bone healing. A range of movement of the knee joint of 0/5/110° was achieved by the last clinical examination 5 years postoperatively. The patient was free of pain and had an age-appropriate mobility. The use of fixed-angle plates in the treatment of type II periprosthetic patellar fractures according to Ortiguera and Berry can provide stable fixation while preserving the retropatellar prosthetic component. Additional augmentation by cerclage, tapes or traction screws is recommended in cases of poor bone stock or severe osteoporosis., (© 2021. Springer Medizin Verlag GmbH, ein Teil von Springer Nature.)

Leisure-time physical activity (LTPA) is associated with various health-promoting effects. However, little is known about the relationship between life events and changes in LTPA over the life course, especially when multiple life events occur simultaneously. Therefore, this study examines taking up and terminating LTPA associated with life events in the familial and occupational life domains over 16 years of 16-76-year-old Swiss inhabitants ( n = 1857) in a retrospective longitudinal cohort design, using a validated telephone survey and multilevel discrete-time event-history analyses. The results show that taking up LTPA was more likely when ending a relationship and retiring and less likely when becoming a parent; terminating LTPA was more likely when ending a job, starting vocational training after 30 years, a relationship ended for men, and becoming a mother with increasing age. If experiencing multiple life events simultaneously, the greater the number of life events, the more likely persons aged 45-70 years were to take up LTPA and, conversely, the more likely persons aged 15-44 years to terminate LTPA. The relationship between life events and changes in LTPA over the life course was often age dependent, especially when experiencing multiple life events simultaneously. The findings should be considered when promoting LTPA.

Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals., (Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

Starch-based isomalto/malto-polysaccharides (IMMPs) are soluble dietary fibres produced by the incubation of α-(1 → 4) linked glucans with the 4,6-α-glucanotransferase (GTFB) enzyme. In this study, we investigated the reaction dynamics of the GTFB enzyme by using isoamylase debranched starches as simplified linear substrates. Modification of α-glucans by GTFB was investigated over time and analysed with 1 H NMR, HPSEC, HPAEC combined with glucose release measurements. We demonstrate that GTFB modification of linear substrates followed a substrate/acceptor model, in which α-(1 → 4) linked glucans DP ≥ 6 functioned as donor substrate, and α-(1 → 4) linked malto-oligomers DP < 6 functioned as acceptor. The presence of α-(1 → 4) linked malto-oligomers DP < 6 resulted in higher GTFB transferase activity, while their absence resulted in higher GTFB hydrolytic activity. The information obtained in this study provides a better insight into GTFB reaction dynamics and will be useful for α-glucan selection for the targeted synthesis of IMMPs in the future., (Copyright © 2021. Published by Elsevier B.V.)

In this study, we present an enzymatic fingerprinting method for the characterization of isomalto/malto-polysaccharides (IMMPs). IMMPs are produced by the modification of starch with the 4,6-α-glucanotransferase (GTFB) enzyme and consist of α-(1→4), α-(1→6) and α-(1→4,6) linked glucoses. Enzymes were used separately, simultaneously or in successive order to specifically degrade and/or reveal IMMP substructures. The enzymatic digests were subsequently analysed with HPSEC and HPAEC to reveal the chain length distribution (CLD) of different IMMP substructures. The presence of amylose in the substrate resulted in the formation of linear α-(1→6) linked glycosidic chains (13.5 kDa) in the former amylopectin fraction. The length of these chains indicates that GTFB transferase activity on amylopectin is more likely to elongate single amylopectin chains than to provide an even distribution. Enzymatic fingerprinting also revealed that the GTFB enzyme is capable of introducing large (20 kDa) linear α-(1→6) linked glycosidic chains in the α-glucan substrate., (Copyright © 2018 Elsevier Ltd. All rights reserved.)