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Your search keyword '"Kirk Czymmek"' showing total 5 results
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5 results on '"Kirk Czymmek"'

4. High Light and High Temperature Reduce Photosynthesis via Different Mechanisms in the C4 Model Setaria viridis

Author

Cheyenne Anderson, Erin Mattoon, Ningning Zhang, Eric Becker, William McHargue, Jiani Yang, Dhruv Patel, Oliver Dautermann, Scott McAdam, Tonantzin Tarin, Sunita Pathak, Tom Avenson, Jeffrey Berry, Maxwell Braud, Krishna Niyogi, Margaret Wilson, Dmitri Nusinow, Rodrigo Vargas, Kirk Czymmek, Andrea Eveland, and Ru Zhang

Abstract

The authors have requested that this preprint be removed from Research Square.

Published
2021

5. Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy

Author

Kirk, Czymmek, Abhilash, Sawant, Kaija, Goodman, Janice, Pennington, Pal, Pedersen, Mrinalini, Hoon, and Marisa S, Otegui

Subjects
Freeze Substitution, Arabidopsis, Microscopy, Electron, Scanning, Microtomy
Abstract

This chapter describes methods to enhanced contrast of plant material processed by high-pressure freezing and freeze substitution for improved visualization by serial block-face scanning electron microscopy (SBEM). The contrast enhancing steps are based on a protocol involving the sequential incubation of samples in heavy metals and sodium thiocarbohydrazide (OTO staining). We also describe the pipeline for imaging plant tissues in a commercial SBEM system (Gatan 3View

Published
2020

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