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Your search keyword '"King, David C"' showing total 17 results
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17 results on '"King, David C"'

5. Transcriptome profiling of the Caenorhabditis elegans intestine reveals that ELT-2 negatively and positively regulates intestinal gene expression within the context of a gene regulatory network

Author

Williams, Robert T P, primary, King, David C, additional, Mastroianni, Izabella R, additional, Hill, Jessica L, additional, Apenes, Nicolai W, additional, Ramirez, Gabriela, additional, Miner, E Catherine, additional, Moore, Andrew, additional, Coleman, Karissa, additional, and Nishimura, Erin Osborne, additional

Published
2023
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10. Genomic characterization of 99 viruses from the bunyavirus families Nairoviridae, Peribunyaviridae, and Phenuiviridae, including 35 previously unsequenced viruses

Author

Kapuscinski, Marylee L., primary, Bergren, Nicholas A., additional, Russell, Brandy J., additional, Lee, Justin S., additional, Borland, Erin M., additional, Hartman, Daniel A., additional, King, David C., additional, Hughes, Holly R., additional, Burkhalter, Kristen L., additional, Kading, Rebekah C., additional, and Stenglein, Mark D., additional

Published
2021
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16. Transcriptome profiling of the Caenorhabditis elegansintestine reveals that ELT-2 negatively and positively regulates intestinal gene expression within the context of a gene regulatory network

Author

Williams, Robert T P, King, David C, Mastroianni, Izabella R, Hill, Jessica L, Apenes, Nicolai W, Ramirez, Gabriela, Miner, E Catherine, Moore, Andrew, Coleman, Karissa, and Nishimura, Erin Osborne

Abstract

ELT-2 is the major transcription factor (TF) required for Caenorhabditis elegansintestinal development. ELT-2 expression initiates in embryos to promote development and then persists after hatching through the larval and adult stages. Though the sites of ELT-2 binding are characterized and the transcriptional changes that result from ELT-2 depletion are known, an intestine-specific transcriptome profile spanning developmental time has been missing. We generated this dataset by performing Fluorescence Activated Cell Sorting on intestine cells at distinct developmental stages. We analyzed this dataset in conjunction with previously conducted ELT-2 studies to evaluate the role of ELT-2 in directing the intestinal gene regulatory network through development. We found that only 33% of intestine-enriched genes in the embryo were direct targets of ELT-2 but that number increased to 75% by the L3 stage. This suggests additional TFs promote intestinal transcription especially in the embryo. Furthermore, only half of ELT-2's direct target genes were dependent on ELT-2 for their proper expression levels, and an equal proportion of those responded to elt-2depletion with over-expression as with under-expression. That is, ELT-2 can either activate or repress direct target genes. Additionally, we observed that ELT-2 repressed its own promoter, implicating new models for its autoregulation. Together, our results illustrate that ELT-2 impacts roughly 20–50% of intestine-specific genes, that ELT-2 both positively and negatively controls its direct targets, and that the current model of the intestinal regulatory network is incomplete as the factors responsible for directing the expression of many intestinal genes remain unknown.

Published
2023
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17. Multifunctional cytomegalovirus (CMV)-specific CD8+ T cells are not restricted by telomere-related senescence in young or old adults.

Author

Riddell, Natalie E., Griffiths, Stephen J., Rivino, Laura, King, David C. B., Teo, Guo H., Henson, Sian M., Cantisan, Sara, Solana, Rafael, Kemeny, David M., MacAry, Paul A., Larbi, Anis, and Akbar, Arne N.

Subjects
CYTOMEGALOVIRUS diseases, CD8 antigen, T cells, TELOMERES, CELLULAR aging, INTERFERONS, CELL differentiation, PHYSIOLOGY
Abstract

Antigen-specific multifunctional T cells that secrete interferon- γ, interleukin-2 and tumour necrosis factor- α simultaneously after activation are important for the control of many infections. It is unclear if these CD8+ T cells are at an early or late stage of differentiation and whether telomere erosion restricts their replicative capacity. We developed a multi-parameter flow cytometric method for investigating the relationship between differentiation (CD45RA and CD27 surface phenotype), function (cytokine production) and replicative capacity (telomere length) in individual cytomegalovirus (CMV) antigen-specific CD8+ T cells. This involves surface and intracellular cell staining coupled to fluorescence in situ hybridization to detect telomeres (flow-FISH). The end-stage/senescent CD8+ CD45RA+ CD27− T-cell subset increases significantly during ageing and this is exaggerated in CMV immune-responsive subjects. However, these end-stage cells do not have the shortest telomeres, implicating additional non-telomere-related mechanisms in inducing their senescence. The telomere lengths in total and CMV (NLV)-specific CD8+ T cells in all four subsets defined by CD45RA and CD27 expression were significantly shorter in old compared with young individuals in both a Caucasian and an Asian cohort. Following stimulation by anti-CD3 or NLV peptide, similar proportions of triple-cytokine-producing cells are found in CD8+ T cells at all stages of differentiation in both age groups. Furthermore, these multi-functional cells had intermediate telomere lengths compared with cells producing only one or two cytokines after activation. Therefore, global and CMV (NLV)-specific CD8+ T cells that secrete interferon- γ, interleukin-2 and tumour necrosis factor- α are at an intermediate stage of differentiation and are not restricted by excessive telomere erosion. [ABSTRACT FROM AUTHOR]

Published
2015
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