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Organic micropollutants (OMPs) represent an anthropogenic stressor on stream ecosystems. In this work, we combined passive sampling with suspect and nontarget screening enabled by liquid chromatography-high-resolution mass spectrometry to characterize complex mixtures of OMPs in streams draining mixed-use watersheds. Suspect screening identified 122 unique OMPs for target quantification in polar organic chemical integrative samplers (POCIS) and grab samples collected from 20 stream sites in upstate New York over two sampling seasons. Hierarchical clustering established the co-occurrence profiles of OMPs in connection with watershed attributes indicative of anthropogenic influences. Nontarget screening leveraging the time-integrative nature of POCIS and the cross-site variability in watershed attributes prioritized and confirmed 11 additional compounds that were ubiquitously present in monitored streams. Field sampling rates for 37 OMPs that simultaneously occurred in POCIS and grab samples spanned the range of 0.02 to 0.22 L/d with a median value of 0.07 L/d. Comparative analyses of the daily average loads, cumulative exposure-activity ratios, and multi-substance potentially affected fractions supported the feasibility of complementing grab sampling with POCIS for OMP load estimation and screening-level risk assessments. Overall, this work demonstrated a multi-watershed sampling and screening approach that can be adapted to assess OMP contamination in streams across landscapes.

Climate variations and human modifications of the water cycle continue to alter the Earth's surface water and energy exchanges. It is therefore critical to ascertain how these changes impact water quality and aquatic ecosystem habitat metrics such as river temperatures. Though river temperature trend analyses exist in the literature, studies on seasonal trends in river temperatures across large spatial extents, e.g. the contiguous United States (US), are limited. As we show through both annual and monthly trend analyses for 20 year ( n = 138 sites) and 40 year ( n = 40 sites) periods, annual temperature trends across the US mask extensive monthly variability. While most sites exhibited annual warming trends, these annual trends obscured sub-annual cooling trends at many sites. Monthly trend anomalies were spatially organized, with persistent regional patterns at both reference and human-impacted sites. The largest warming and cooling anomalies happened at human impacted sites and during summer months. Though our analysis points to coherence in trends as well as the overall impact of human activity in driving these patterns, we did not investigate the impact of river temperature observation accuracy on reported trends, an area needed for future work. Overall, these patterns emphasize the need to consider sub-annual behavior when managing the ecological impacts of river temperature throughout lotic networks.

Background: Viral capsid assembly involves the oligomerization of the capsid nucleoprotein (NP), which is an essential step in viral replication and may represent a potential antiviral target. An in vitro transcription-translation reaction using a wheat germ (WG) extract in combination with a sandwich ELISA assay has recently been used to identify small molecules with antiviral activity against the rabies virus., Results: Here, we examined the application of this system to viruses with capsids with a different structure, such as the Rift Valley fever virus (RVFV), the etiological agent of a severe emerging infectious disease. The biochemical and immunological characterization of the in vitro-generated RVFV NP assembly products enabled the distinction between intermediately and highly ordered capsid structures. This distinction was used to establish a screening method for the identification of potential antiviral drugs for RVFV countermeasures., Conclusions: These results indicated that this unique analytical system, which combines nucleoprotein oligomerization with the specific immune recognition of a highly ordered capsid structure, can be extended to various viral families and used both to study the early stages of NP assembly and to assist in the identification of potential antiviral drugs in a cost-efficient manner., Reviewers: Reviewed by Jeffry Skolnick and Noah Isakov. For the full reviews please go to the Reviewers' comments section.