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Vascular endothelial cell injury induced by high glucose (HG) plays an important role in the occurrence and development of diabetic vascular complications. Yellow tea has a protective effect on vascular endothelial cells. However, the molecular mechanisms underlying this effect are unclear. In this study, the effects of the

MicroRNAs have been reported to be implicated in myocardial ischemia/reperfusion (I/R) injury. The purpose of this study was to investigate the effect of miR-183-5p on I/R injury. Overexpression of miR-183-5p by agomiR transfection alleviated cardiac dysfunction and significantly reduced the infarct size in rats with myocardial I/R. MiR-183-5p also alleviated myocardial apoptosis with reduced apoptotic cells and lower levels of apoptosis associated proteins. in vitro experiments were conducted on rat H9c2 cells treated with anoxia/reoxygenation (A/R). Annexin V/propidium iodide (PI) staining and flow cytometry reported that the ratio of apoptotic cells decreased by miR-183-5p transfection before A/R treatment. Moreover, according to binding sequence prediction and Dual luciferase reporter assay, we explored that voltage-dependent anion channel 1 (VDAC1), which aggravates myocardial injury and apoptosis reported in our former research, was a target of miR-183-5p. In conclusion, miR-183-5p can efficiently attenuate I/R injury and miR-183-5p may exert its effect through repressing VDAC1 expression.

To observe the relationship between pulmonary artery systolic pressure (PASP) and acute renal injury (AKI) and prognosis after cardiopulmonary bypass (CPB) heart surgery.The clinical data of 9 860 patients who underwent CPB heart surgery in Beijing Anzhen Hospital from January 1st, 2015 to December 31st, 2016 were analyzed retrospectively. The patients were divided into two groups according to whether AKI occurred after operation. The clinical data were obtained from hospital information system (HIS) and DoCare including general information, types of operation, preoperative complication, ejection fraction, serum creatinine (SCr), PASP, intraoperative CPB duration, aortic occlusion duration, fluid balance, blood products and drug usage, postoperative mechanical ventilation duration, length of intensive care unit (ICU) and hospital stay, and perioperative central venous pressure (CVP). Multivariate Logistic regression analysis was used to screen the risk factors of AKI after operation. According to the preoperative PASP level, the patients were divided into ≥ 60 mmHg (1 mmHg = 0.133 kPa) group and60 mmHg group, and the incidence of AKI and prognosis after operation were compared between the two groups. All patients were followed up by telephone after discharge, and they were divided into survival group and death group according to the follow-up results, and the clinical data were compared between the two groups. Multivariate Cox regression analysis was used to screen the risk factors of long-term prognosis. Kaplan-Meier survival curve was used to analyze the long-term prognosis of two groups with different preoperative PASP levels.6 285 patients were enrolled in the final analysis. (1) Among the 6 285 patients, 2 592 patients (41.2%) suffered from AKI after operation, of whom 1 697 (65.5%) were stage 1 according to Kidney Disease: Improving Global Outcomes (KDIGO), which was the main type of AKI. Univariate analysis showed that age, preoperative ejection fraction, SCr, PASP, coronary heart disease, hypertension, diabetes, intraoperative CPB duration, aortic occlusion duration, fluid balance, red blood cell input and norepinephrine, dopamine, epinephrine dosage, postoperative mechanical ventilation duration, the length of ICU and hospital stay, and perioperative CVP might be the risk factors of AKI after operation. Multivariate Logistic regression analysis showed that preoperative PASP was one of independent risk factors for AKI in patients undergoing CPB heart surgery [odds ratio (OR) = 4.753, 95% confidence interval (95%CI) was 1.328-8.417, P = 0.004]. The incidence of AKI after operation in PASP ≥ 60 mmHg group was significantly higher than that in60 mmHg group [73.8% (712/965) vs. 35.3% (1 880/5 320), P0.01]. (2) After a follow-up of (11±3) months, 237 patients (3.8%) died in 6 285 patients. The mortality of patients in PASP ≥ 60 mmHg group was significantly higher than that in60 mmHg group [9.5% (92/965) vs. 2.7% (145/5 320), P0.01]. Kaplan-Meier survival curve analysis showed that there was a significant difference between the two groups in cumulative survival rate (Log-Rank test: χThe increase of PASP is related to AKI after CPB heart surgery, which is an independent risk factor for long-term mortality.

Background Hyperlipidemia characterized of elevated serum lipid levels is a prevalent disease frequently resulting in cardiovascular disease (CVD). Berberine and evodiamine are herbal products of traditional Chinese herb Coptis chinensis and Evodia rutaecarpa, which are indicated to exert regulation of lipid metabolism. Therefore, the objective of this study was to investigate the lipid-lowering effect of berberine and evodiamine combination in hyperlipidemic rats. Method The rat model of hyperlipidemia was established by providing high-fat-diet (HFD) for 4 weeks. Berberine (BB), evodiamine (EV), and their combination (BB + EV) were orally administered to HFD induced rats for 4 weeks. Body weight, food utilization, histopathology of liver tissues, lipid profiles of serum and liver were measured. Gas chromatography (GC) analysis was applied to examine the level of plasma total cholesterol and ß- Sitosterol (BS) to estimate cholesterol absorption activity. Furthermore, intestinal NPC1L1, ACAT2, and ApoB48 protein expressions were evaluated by immunohistochemical assay. Result According to the results, decreased levels of serum cholesterol (TC), triglycerides (TG), low density lipoprotein-cholesterol (LDL-C), as well as hepatic TC were showed in hyperlipidemic rats treated by combination of berberine and evodiamine. GC analysis indicated that the elevated plasma BS was significantly ameliorated by BB, EV, and BB + EV. In addition, immunohistochemical analysis revealed that BB + EV treatment down-regulated the expressions of intestinal NPC1L1 and ACAT2, and ApoB48 in HFD induced rats. Conclusion Based on the above results, combination of berberine and evodiamine exerted a promising preventive effect on hyperlipidemia, partially through inhibiting intestinal absorption of cholesterol.

In this paper model predictive direct torque control (MP-DTC) for surface permanent magnet synchronous motor (PMSM) direct torque control (DTC) system is proposed. Based on flux and torque's expressions of surface PMSM in stator flux reference frame, 6 basic voltage vectors generated by voltage source inverter (VSI) are used as candidate voltage vectors. And according to values of stator flux and torque at next sampling point after applying these 6 voltage vector, 6 different values of cost function about stator flux and torque's error are obtained. The predictive control will select voltage vector corresponding to the minimum cost function as output voltage vector. And in order to reduce the computational burden, based on the utilization rate of 6 voltage vectors, the predictive control using simplified aggregate of candidate voltage vectors is given. Simulation results show compared with switching table, control performance of predictive control is better. And compared with predictive control using 6 basic voltage vectors, the proposed predictive control has almost the same control performance, but switching frequency is decreased and calculation burden is also reduced.

Based on stator flux and torque's expressions of interior permanent magnet synchronous motor (PMSM) direct torque control (DTC) system in stator flux frame, voltage vector with variable amplitude and fixed angle is used as candidate output voltage vector. The amplitude of stator flux and torque at next sampling point after applying voltage vector with different amplitude are obtained. A one-step selection algorithm is established based on the cost function of stator flux and torque's errors, which can choose voltage vector to minimize the cost function. More candidate voltage vectors will lead to better control performance, but it will cause heavier calculation burden. In this paper, control performances of interior PMSM DTC system are compared using 10 different methods to divide voltage vector. Considering control performance and calculation burden, dividing voltage vector into five equal parts to get the aggravate of 5 candidate voltage vectors is an ideal way. And system performance tends to be stable.

Based on flux and torque’s expressions of surface permanent magnet synchronous motor (PMSM) direct torque control (DTC) system, values of stator flux and torque at next sampling point after applying voltage vector with different angle are given in this paper. The objective function is proposed which aims to minimize stator flux and torque’s error. The predictive control will select the optimal angle corresponding to the minimum objective function. Simulation results show the surface PMSM DTC system under the predictive control works properly. The predictive control selects the optimal voltage vector from aggregate of voltage vectors with different angle. More selectable voltage vectors will lead to better control performance, but it will cause heavier calculation burden. In this paper, control performances of surface PMSM DTC system using different aggregates of voltage vectors are studied. Considering control performance and calculation burden, an ideal aggregate of voltage vector is given.

Based on flux and torque’s expressions of surface permanent magnet synchronous motor (PMSM) direct torque control (DTC) system, the values of stator flux and torque at next sampling point after applying voltage vector with different amplitude are given in this paper. And objective function of flux and torque’s error is proposed which only uses the amplitude of applying voltage vector as the variable. The predictive control will select the optimal voltage vector which aims to minimize objective function. Simulation results show the PMSM DTC system under the control of proposed strategy works properly. The predictive control selects the optimal voltage vector from aggregate of voltage vectors. More selectable voltage vectors will lead to better control performance, but it will cause heavier calculation burden. In this paper, control performances of surface PMSM DTC system are given using 9 aggregates of selectable voltage vectors. Considering control performance and calculation burden, dividing voltage vector into three equal parts to get 3 selectable voltage vectors is an ideal way.

Penehyclidine hydrochloride (PHC) preconditioning can alleviate myocardial ischemia/reperfusion (I/R) injury and inhibits the upregulation of voltage-dependent anion channel 1 (VDAC1) during I/R. To validate that VDAC1 is a bona fide target of PHC for the protection against myocardial I/R injury, VDAC1 expression construct was delivered by lentiviruses into rat left ventricular myocardium before PHC preconditioning and myocardial I/R. Overexpression of VDAC1 exacerbated cardiac dysfunction and myocardial injury following I/R, and abolished the cardioprotective effect of PHC during I/R injury. Moreover, VDAC1 overexpression with myocardial I/R further increased cytochrome c release from mitochondria to cytoplasm, elevated the levels of cleaved caspase-3 and Bax, and decreased the level of Bcl-2 as compared with I/R alone, and PHC-mediated inhibition of mitochondria-dependent apoptosis during myocardial I/R was abolished by VDAC1 overexpression. In addition, VDAC1 was overexpressed in H9c2 cardiomyocytes undergoing anoxia/reoxygenation (A/R) with or without PHC pretreatment. The in vitro results showed that overexpression of VDAC1 further reduced mitochondrial membrane potential, increased mitochondrial membrane permeability and enhanced mitochondria-dependent apoptosis in H9c2 cells after A/R, and VDAC1 overexpression abrogated the protective effect of PHC on the mitochondrial function and integrity during A/R. In conclusion, exogenous overexpression of VDAC1 during myocardial I/R inhibits the cardioprotective effects of PHC. These effects may be associated with the suppression of VDAC1 expression.

In the early days of bar coding, an Efficient Foodservice Response (EFR) study identified $847 million in savings. Today, technologies, techniques, and standards exist to help organizations throughout the food supply chain gain complete traceability for safety, compliance, and business process improvement. RF identification systems use RF tag to transmit information between RF tags and reading devices through sensors, radio wave or microwave for non-contact bidirectional communications, in order to identify objects automatically. This write paper examines how the food industry can take advantage of RFID technology to improve safety, reduce operating expenses, meet compliance requirements, and improve efficiency.