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Abstract The growing energy demand and lack of access to clean energy sources call for the development of more energy for cooking in households, briquettes produced using agricultural materials and related biomass have the capacity for utilization as an energy source. This study aims to introduce briquettes as an alternative energy source for cooking within conflict-affected communities in Borno State, northeastern Nigeria, where factors relating to the high cost of charcoal, security constraints, and the environmental impact of tree cutting have sparked apprehension and raised concerns. The study demonstrates the likelihood of adopting briquettes as a cooking fuel alternative in communities recovering from insurgency. Using assessment tools such as household surveys, Key Informant Interviews (KIIs), and Focus Group Discussions (FGDs), involving a total of 536 questionnaires administered to respondents; KII was administered to 37 respondents and 9 FGDs with cooperatives and dealers in traditional fuel were conducted. From the results 73% of the community relies on charcoal as their primary fuel source, since the majority (68.7%) appeared to be low-income households, 24.1% reported that the high cost of charcoal is a major hindrance to continued usage. However, despite the communities showing potential in briquettes, only 3.7% are aware of briquettes as an alternative option for cooking energy indicating the adoption of briquettes being low in the region. A situational assessment was carried and sensitization strategies were recommended as means to enhance livelihoods and build resilience within the community.

In recent years, overexplorations of ore and the growth of industries are the prime factors in the release of heavy metals in environments. As a result, the food crops and water bodies are contaminated with metals which may have several adverse effects on the health of humans and other living species. These metals and metalloids, such as Zn, Cu, Mn, Ni, Cr, Pb, Cd, and As, upset the biochemical pathways of metabolite synthesis in living organisms and contribute to the etiology of different diseases. Microorganisms include bacteria, archaea, viruses, and many unicellular eukaryotes, which can span three domains of life—Archaea, Bacteria, and Eukarya—and some microorganisms, such as cyanobacteria, have shown high efficiency in the biosorption rate of heavy metals. Cyanobacteria are suitable for bioremediation as they can grow in adverse environments, have a less negative impact on the surrounding environment, and are relatively cheaper to manage. The structure of cyanobacteria has shown no extensive internal-bound membranes, so it can directly employ the physiological mechanisms to uptake heavy metals from contamination sites. Such biochemical makeups are suitable for managing and bioremediating heavy metal concentrations in polluted environments. This review aims to explore the potential of cyanobacteria in the bioremediation of heavy metals and metalloids in water bodies. Additionally, we have identified the prospects for enhancing bioremediation effectiveness.

In this paper, we introduce the notions of mean chain transitivity, mean chain mixing, totally mean chain transitivity, and almost mean shadowing property to iterated function systems (IFS). We study the interrelations of these notions. We prove that an iterated function system is chain transitive if one of the constituent maps is surjective, and it has almost mean shadowing property.

Abstract Greenspaces are important for sustaining healthy urban environments and their human populations. Yet their capacity to support multiple ecosystem services simultaneously (multiservices) compared with nearby natural ecosystems remains virtually unknown. We conducted a global field survey in 56 urban areas to investigate the influence of urban greenspaces on 23 soil and plant attributes and compared them with nearby natural environments. We show that, in general, urban greenspaces and nearby natural areas support similar levels of soil multiservices, with only six of 23 attributes (available phosphorus, water holding capacity, water respiration, plant cover, arbuscular mycorrhizal fungi (AMF), and arachnid richness) significantly greater in greenspaces, and one (available ammonium) greater in natural areas. Further analyses showed that, although natural areas and urban greenspaces delivered a similar number of services at low (>25% threshold) and moderate (>50%) levels of functioning, natural systems supported significantly more functions at high (>75%) levels of functioning. Management practices (mowing) played an important role in explaining urban ecosystem services, but there were no effects of fertilisation or irrigation. Some services declined with increasing site size, for both greenspaces and natural areas. Our work highlights the fact that urban greenspaces are more similar to natural environments than previously reported and underscores the importance of managing urban greenspaces not only for their social and recreational values, but for supporting multiple ecosystem services on which soils and human well-being depends.

This paper aims to improve the design of a seat slider mechanism for an automotive seating system. The seating track mechanism comprises various parts, such as the track bar, upper slider rail, lower slider rail, and locking/unlocking mechanism. The upper rail holds the seat and manikin weight, whereas the lower rail is secured with mounting brackets. A Computer-aided design model of the proposed modified locking/unlocking mechanism of the seating track is created and conducted Finite Element Analysis (FEA). Also, the study includes the motion and dynamic analysis of the seating track mechanism using the bond graph technique by considering the mechanism with an equivalent spring-mass-damper system. The FEA ensured the design strength and its safety regarding failure analysis, whereas the motion analysis confirmed the suitability of the mechanism's kinematics. It is found that the maximum stress is developed at the track bar, i.e., 186.35 MPa, whereas the track displacement reaches its final design position, i.e., 175 mm in 2 s, when the applied force on the track bar is 100 N and on each slider is 20 N. The study ensures an alternative spring-less locking/unlocking mechanism for the seat slider mechanism.

Drought is the most important abiotic stress that restricts the genetically predetermined yield potential of the crops. In the present study, four tomato varieties: Kashi Vishesh, Kashi Aman, Kashi Abhiman, and Kashi Amrit, were used to study the effect of PGPMs (plant growth-promoting microorganisms). PGPM strains, Bacillus megaterium BHUPSB14, Pseudomonas fluorescens BHUPSB06, Pseudomonas aeruginosa BHUPSB01, Pseudomonas putida BHUPSB0, Paenibacillus polymixa BHUPSB17, and Trichoderma horzianum, were used as the consortium. The control group was irrigated up to 80% of field capacity, while 7-, 14-, and 21-day water-deficit-exposed (DWD) plants’ pot soil moisture was maintained to 40, 25, and 15% of the field capacity, both with and without the PGPM inoculation condition. The physiological parameters, such as electrolyte leakage, relative water content, photosynthetic efficiency, and chlorophyll color index, were significantly improved by PGPM application under progressive drought stress, compared to the control. PGPM application enhanced the proline accumulation and reduced the formation of hydrogen peroxide and lipid peroxidation under drought stress. The plant growth attributes were significantly increased by PGPM application. The Kashi Amrit variety showed the highest fruit yield among the four varieties under all the treatments. The PGPM consortium application also improved the soil physico-biological properties and nutrient availability in the soil. The PGPM consortium used in this study can potentially mitigate drought stress on tomato in drought-prone regions and act as a biofertilizer. The present study will open a new avenue of drought stress management in tomato.

Mycoplasma gallisepticum (MG) has been reported to cause significant economic losses to the poultry industry. In this study, real-time TaqMan and conventional MG PCR assays were standardized for rapid and specific detection of pathogen and its efficacy were compared. A total of 146 pooled tissue samples comprising trachea, lungs and air sacs were collected from dead birds having history of respiratory tract infections. Each sample (tissues of 4–5 dead birds) represented different poultry flocks located in Haryana and Rajasthan states of India. The analysis of samples was carried out using primers of 16 S rRNA for conventional and lipoprotein gene for real-time MG PCR assays. In conventional PCR, 48/146 (32.87%) tissue samples were found positive for MG. However, real-time PCR showed higher positivity (58/146; 39.72%) as compared to conventional PCR. Considering the conventional PCR as a gold standard, sensitivity (100%), specificity (89.8%) & positive (0.81) and negative (1.0) predictive values of the standardized real-time PCR was calculated. However, Cohen's kappa statistic (k = 0.85) showed a 'strong' agreement between two PCR assays. The study revealed that screening of samples using more than one molecular technique is helpful for determining true burden of the disease.

Background: Dysmenorrhea, the cramping pain experienced by women during menstruation, can significantly impact their daily lives and academic performance. Mental stress is a potential trigger for dysmenorrhea, but their association remains to be precisely determined. This study aims to explore the prevalence of dysmenorrhea among undergraduate female students at Universal College of Medical Sciences and investigate its association with perceived stress. Methods: A descriptive cross-sectional study was conducted among undergraduate female students at Universal College of Medical Sciences, Bhairahawa. Participants were selected using a simple random sampling method. A structured questionnaire, including anthropometric data, menstrual history, and the Perceived Stress Scale (PSS), were used for data collection. PSS scores were used to categorize stress levels as low, moderate, or high. Results: Total 196 female students participated in the study. Dysmenorrhea was present in 80.1% of the participants, with 34.7% seeking treatment for the condition. Additionally, 29.6% of the students reported moderate to severe premenstrual symptoms. Most students (81.6%) had a moderate level of perceived stress. Statistical analysis revealed a significant association between dysmenorrhea and level of perceived stress (p < 0.001). Conclusion: The study findings highlight a considerable prevalence of dysmenorrhea among female students at Universal College. The association between dysmenorrhea and perceived stress underscores the importance of addressing the psychosocial well-being of these students. Implementing supportive measures, such as mental health support and stress management programs, can create a more conducive environment for students to manage dysmenorrhea and its impact on their academic performance.

ABSTRACT Biological soil crusts (BSC) are commonly found in soils in the drylands regions, which can influence stabilization, water retention, nutrient cycling (particularly carbon (C) and nitrogen (N) dynamics), and several ecological processes. However, the composition of BSC in Brazilian soils undergoing the desertification process remains poorly understood. This study aimed to characterize the bacterial community in BSC formed in a Brazilian semiarid region under the desertification process. Thus, a highly desertified region was selected from which 34 BSC samples were collected. The total DNA of the BSC was extracted from 0.5 g samples, and the bacterial community was sequenced by a Next Generation Sequencing (NGS) platform (Miseq – Illumina®) using universal primers (515F and 806R). Bioinformatic analysis was carried out in QIIME (v.1.9), and the Operational Taxonomic Units (OTU) table was constructed following the Sumaclust methodology. The pH of BSC, C, N, and phosphorus contents was analyzed. Our study identified a diverse bacterial community in the BSCs. Cyanobacteria, Chloroflexi, and Proteobacteria phyla presented the greatest relative abundance (%) across the samples. Cyanobacteria were dominated by the orders Nostocales and Leptolyngbyales. The prediction of the putative functions found that mostf OTU were related to phototrophy, photosynthetic cyanobacteria, and photoautotrophy. The study found correlations between bacterial phyla and BSC properties, with Cyanobacteria positively related to C. Chloroflexi, Armatimonadetes, and WPS-2 were negatively correlated with C and N contents. These results suggest the critical roles bacteria communities play in BSCs from the Caatinga biome and highlight the potential impact of environmental factors on their diversity and functions.

This review aims to examine the existence of Pseudomonas aeruginosa (P. aeruginosa) and their antibiotic resistance genes (ARGs) in aquatic settings and the alternative treatment ways. P. aeruginosa in a various aquatic environment have been identified as contaminants with impacts on human health and the environment. P. aeruginosa resistance to multiple antibiotics, such as sulfamethoxazole, ciprofloxacin, quinolone, trimethoprim, tetracycline, vancomycin, as well as specific antibiotic resistance genes including sul1, qnrs, blaVIM, blaTEM, blaCTX, blaAIM-1, tetA, ampC, blaVIM. The development of resistance can occur naturally, through mutations, or via horizontal gene transfer facilitated by sterilizing agents. In addition, an overview of the current knowledge on inactivation of Pseudomonas aeruginosa and ARG and the mechanisms of action of various disinfection processes in water and wastewater (UV chlorine processes, catalytic oxidation, Fenton reaction, and ozonation) is given. An overview of the effects of nanotechnology and the resulting wetlands is also given.

Rice is a major dietary element for about two billion people worldwide and it faces numerous biotic and abiotic stress for its cultivation. Rice blast disease caused by Magnaporthe oryzae reduce up to 30 % rice yield. Overuse of synthetic chemicals raises concerns about health and environment; so, there is an urgent need to explore innovative sustainable strategies for crop productivity. The main aim of this study is to explore the impact of bacterial volatiles (BVCs) on seedling growth and defense mechanisms of rice under in-vitro condition. On the basis of plant growth promoting properties, six bacterial strains were selected out of ninety-one isolated strains for this study; Pantoea dispersa BHUJPVR01, Enterobacter cloacae BHUJPVR02, Enterobacter sp. BHUJPVR12, Priestia aryabhattai BHUJPVR13, Pseudomonas sp. BHUJPVWRO5 and Staphylococcus sp. BHUJPVWLE7. Through the emission of bacterial volatiles compounds (BVCs), Enterobacter sp., P. dispersa and P. aryabhattai significantly reduces the growth of rice blast fungus Magnaporthe oryzae by 69.20 %, 66.15 % and 62.31 % respectively. Treatment of rice seedlings with BVCs exhibited significant enhancement in defence enzyme levels, including guaiacol peroxidase, polyphenol oxidase, total polyphenols, and total flavonoids by a maximum of up to 24 %, 48 %, 116 % and 80 %, respectively. Furthermore, BVCs effectively promote shoot height, root height, and root counts of rice. All BVCs treated plant showed a significant increase in shoot height. P. dispersa treated plants showed the highest increase of 60 % shoot and 110 % root length, respectively. Root counts increased up to 30% in plants treated with E. cloacae and Staphylococcus sp. The BVCs can be used as a sustainable approach for enhancing plant growth attributes, productivity and defence mechanism of rice plant under biotic and abiotic stresses.

OBJECTIVES:. We postulate that corticosteroid-related side effects in critically ill patients are similar across sepsis, acute respiratory distress syndrome (ARDS), and community-acquired pneumonia (CAP). By pooling data across all trials that have examined corticosteroids in these three acute conditions, we aim to examine the side effects of corticosteroid use in critical illness. DATA SOURCES:. We performed a comprehensive search of MEDLINE, Embase, Centers for Disease Control and Prevention library of COVID research, CINAHL, and Cochrane center for trials. STUDY SELECTION:. We included randomized controlled trials (RCTs) that compared corticosteroids to no corticosteroids or placebo in patients with sepsis, ARDS, and CAP. DATA EXTRACTION:. We summarized data addressing the most described side effects of corticosteroid use in critical care: gastrointestinal bleeding, hyperglycemia, hypernatremia, superinfections/secondary infections, neuropsychiatric effects, and neuromuscular weakness. DATA SYNTHESIS:. We included 47 RCTs (n = 13,893 patients). Corticosteroids probably have no effect on gastrointestinal bleeding (relative risk [RR], 1.08; 95% CI, 0.87–1.34; absolute risk increase [ARI], 0.3%; moderate certainty) or secondary infections (RR, 0.97; 95% CI, 0.89–1.05; absolute risk reduction, 0.5%; moderate certainty) and may have no effect on neuromuscular weakness (RR, 1.22; 95% CI, 1.03–1.45; ARI, 1.4%; low certainty) or neuropsychiatric events (RR, 1.19; 95% CI, 0.82–1.74; ARI, 0.5%; low certainty). Conversely, they increase the risk of hyperglycemia (RR, 1.21; 95% CI, 1.11–1.31; ARI, 5.4%; high certainty) and probably increase the risk of hypernatremia (RR, 1.59; 95% CI, 1.29–1.96; ARI, 2.3%; moderate certainty). CONCLUSIONS:. In ARDS, sepsis, and CAP, corticosteroids are associated with hyperglycemia and probably with hypernatremia but likely have no effect on gastrointestinal bleeding or secondary infections. More data examining effects of corticosteroids, particularly on neuropsychiatric outcomes and neuromuscular weakness, would clarify the safety of this class of drugs in critical illness.

Chickpeas (Cicer arietinum L.) are used as a good source of proteins and energy in the diets of various organisms including humans and animals. Chickpea straws can serve as an alternative option for forage for different ruminants. This research mainly focussed on screening the effects of adding beneficial chickpea seed endophytes on increasing the nutritional properties of the different edible parts of chickpea plants. Two efficient chickpea seed endophytes (Enterobacter sp. strain BHUJPCS-2 and BHUJPCS-8) were selected and applied to the chickpea seeds before sowing in the experiment conducted on clay pots. Chickpea seeds treated with both endophytes showed improved plant growth and biomass accumulation. Notably, improvements in the uptake of mineral nutrients were found in the foliage, pericarp, and seed of the chickpea plants. Additionally, nutritional properties such as total phenolics (0.47, 0.25, and 0.55 folds), total protein (0.04, 0.21, and 0.18 folds), carbohydrate content (0.31, 0.32, and 0.31 folds), and total flavonoid content (0.45, 027, and 0.8 folds) were increased in different parts (foliage, pericarp, and seed) of the chickpea plants compared to the control plants. The seed endophyte-treated plants showed a significant increase in mineral accumulation and improvement in nutrition in the different edible parts of chickpea plants. The results showed that the seed endophyte-mediated increase in dietary and nutrient value of the different parts (pericarp, foliage, and seeds) of chickpea are consumed by humans, whereas the other parts (pericarp and foliage) are used as alternative options for forage and chaff in livestock diets and may have direct effects on their nutritional conditions.

Abstract Background Echocardiography has become an integral part of the management of critically ill patients. It helps to diagnose and treat various conditions. COVID-19 patients can develop cardiac dysfunction. We planned to study the echocardiographic parameters in COVID-19 patients. Methods We conducted a prospective observational multicenter study after institutional ethical committee approval. COVID-19 pneumonia patients admitted to the intensive care unit (ICU) were enrolled. The echocardiographic evaluation was done within 24–48 hours of admission. Assessment of the left and right heart with systolic and left ventricular diastolic function evaluation was done. The primary outcome was ICU mortality. The secondary outcomes were the length of ICU stay and duration of mechanical ventilation. Results Among 573 patients mean age was 57.17 (14.67) with 68.60% being males. On day 1 of ICU, invasive mechanical ventilation was used in 257 (45%) patients. One hundred and forty-eight (25.83%) patients were on vasopressors when echocardiography was performed. Severe left ventricle (LV) systolic dysfunction was seen in 8.7% of patients and had higher odds of mortality [2.48(1.058–5.807), p = 0.037] followed by E and e′ with odds ratio of [0.984(0.971–0.998), p = 0.021] and 0.897 (0.805–0.998), p = 0.046], respectively. E/e′ indicative of filling pressure of the LV was not found to be significant. Troponin I, E/A, and RV dilatation were similar among survivors and non-survivors. Conclusion Echocardiographic evaluation in COVID-19 patients showed severe LV systolic dysfunction was associated with ICU mortality. E/e′ was not found to be significant but lower e′ was associated with higher mortality. Trial registration IEC 131/2020, CTRI/2020/06/025858 date 13th June 2020

Long-term afforestation programmes are continuously practiced by State Forest Departments (SFDs) to increase forest cover in India. A large quantity of quality seeds is required for producing quality planting materials every year. The development of seed production areas (SPAs) is a method that can be used to obtain genetically improved seeds for immediate planting. Teak (Tectona grandis Linn. f) is one of the species preferred by SFDs in India for plantation purposes due to its high timber value. Previously, the Directorate of Forests, West Bengal, established ten Seed Stands (SSs) of teak in three Silviculture divisions of West Bengal. In the present study, these SSs (five SSs in the Hills Division, four SSs in the North Plains Division and one SS in the South Division) were evaluated by verification on the basis of area, tree density, isolation zone and tree health. SSs were studied thoroughly to recommend culling inferior trees to convert them to SPAs. Trees with poor phenotypic characteristics, such as slow growth, crooked stem form, attack by pest or disease, were recommended for culling. Out of the ten SSs, five (2/TG/BAMON, 3/TG/BAMON, 3/TG/TUKR, 8/TG/REHTI and 9/TG/ATIA) were considered to be SPAs. Cumulatively, 8.86 hectares of land with 829 trees from the hills division and 15 ha of land with 1712 trees from the North Plains division were chosen for inclusion in the SPAs. Phenotypic data estimation revealed that the maximum average height was 8/TG/REHTI (33.19±2.65 m), and the average GBH was 2/TG/BAMON (2.02±0.54 m). The seed production capability of the selected SPAs was also calculated. On the basis of the average seed yield/tree, the 8/TG/REHTI stand produced the highest seed yield of 2.0 (±0.9) kg/tree.

Using messages posted on Twitter, this study develops a new approach to estimating collective emotions (CEs) within countries. It applies time series methodology to develop and demonstrate a novel application of CEs to identify emotional events that are significant at the societal level. The study analyzes over 200 million words from over 10 million Twitter messages posted in 16 countries during the first 120 days of the COVID-19 pandemic. Daily levels of collective anxiety and positive emotions were estimated using Linguistic Inquiry and Word Count's (LIWC) psychologically validated lexicon. The time series estimates of the two collective emotions were analyzed for structural breaks, which mark a significant change in a series due to an external shock. External shocks to collective emotions come from events that are of shared emotional relevance, and this study develops a new approach to identifying them. In the COVID-19 Twitter posts used in the study, analysis of structural breaks showed that in all 16 countries, a reduction in collective anxiety and an increase in positive emotions followed the WHO's declaration of COVID-19 as a global pandemic. Announcements of economic support packages and social restrictions also had similar impacts in some countries. This indicates that the reduction of uncertainty around the evolving COVID-19 situation had a positive emotional impact on people in all the countries in the study. The study contributes to the field of CEs and applied research in collective psychological phenomena.

Open source software has gained significant traction in recent years, along with some concerns. Here we discuss the features open source model like greater transparency, cost-effectiveness, and customization. It also addresses the rising concerns on privacy and security issues in internet. This article highlights the benefits and limitations of open source software in medical services and health education.

Abstract Soil contamination is one of the main threats to ecosystem health and sustainability. Yet little is known about the extent to which soil contaminants differ between urban greenspaces and natural ecosystems. Here we show that urban greenspaces and adjacent natural areas (i.e., natural/semi-natural ecosystems) shared similar levels of multiple soil contaminants (metal(loid)s, pesticides, microplastics, and antibiotic resistance genes) across the globe. We reveal that human influence explained many forms of soil contamination worldwide. Socio-economic factors were integral to explaining the occurrence of soil contaminants worldwide. We further show that increased levels of multiple soil contaminants were linked with changes in microbial traits including genes associated with environmental stress resistance, nutrient cycling, and pathogenesis. Taken together, our work demonstrates that human-driven soil contamination in nearby natural areas mirrors that in urban greenspaces globally, and highlights that soil contaminants have the potential to cause dire consequences for ecosystem sustainability and human wellbeing.

Background and Aims: Postoperative pain for patients having hip arthroplasty ranges from moderate to severe. Many regional anaesthesia procedures treat postoperative pain to improve functional ability and quality of life. Evidence comparing the analgesic effects of the pericapsular nerve group (PENG) block and fascia iliaca compartment block (FICB) remains unclear. The analgesic efficacies of PENG and FICB in hip arthroplasty were compared to determine which technique is associated with superior analgesia. Methods: The electronic databases (PubMed, Cochrane Library, Google Scholar and Web of Sciences) were searched for published randomised controlled trials (RCTs) till 5 April 2023 comparing PENG block vs. FICB following hip arthroplasty. The primary outcome was pain scores [numerical rating scale (NRS) or visual analogue scale (VAS)] between 0 and 10 at rest and during movement at 24 h. Secondary outcomes included pain scores at rest and during movement within 30 min, at 6 h and 12 h, time to first rescue analgesia and cumulative postoperative opioid use in 24 h. We assessed the risk of bias using the Cochrane Collaboration Risk-of-Bias 2 tool. Using Grading of Recommendations Assessment, Development, and Evaluation (GRADE), the certainty of the evidence was assessed. Subgroup analysis was performed to explore the source of heterogeneity. Results: We included 12 RCTs examining 644 patients. Pain scores at rest at 24 h (standardised mean differences (SMDs): 0.17; 95% confidence interval (CI): -0.90 to 1.23; P = 0.76, moderate certainty) and during movement at 24 h (SMD: -0.58, 95% CI: -1.53 to 0.38, P = 0.24, moderate certainty) were not different in both PENG block and FICB. Pain scores at rest and during movement within 30 min may be lower with PENG block than FICB. However, the pain score at rest and during movement at 6 h and the time to first rescue analgesia were not different between the two treatment arms. The mean opioid consumption in oral morphine equivalents (mg) in 24 h may be lower with PENG than FICB. Conclusion: We observed no difference between the PENG block and the FICB at 24 h for pain at rest and movement with a moderate degree of certainty. However, PENG block showed improved analgesia within 30 min at rest and during movement, and reduce postoperative opioid consumption in 24 h with moderate certainty of evidence. Further large-scale and high-quality RCTs are required to supplement the present findings.

Background and Aims: Sepsis-induced immunosuppression appears to be reversible with immunomodulatory drugs. Mycobacterium indicus pranii (MIP) stimulates the Th1 type of immune response. This systematic review and meta-analysis of randomised controlled trials (RCTs) was aimed to find out if MIP is effective at improving clinical outcomes in sepsis patients. Methods: The databases (PubMed, Google Scholar, Web of Science, and Cochrane Library), along with preprint servers until June 2023, were searched. The methodology was evaluated using the 'Cochrane Collaboration risk of bias-2 tool' for RCT. The study included patients more than 18 years of age with sepsis within 48 h of first organ dysfunction. The primary outcome was 28-day mortality, and secondary outcomes were the length of stay in the intensive care unit (ICU), days on vasopressor support, ventilator-associated pneumonia (VAP), secondary infections, catheter-related bloodstream infections (CRBSI), and the delta sequential organ failure assessment (SOFA) score. Results: The meta-analysis included two studies with 252 participants. In a pooled analysis, mortality in the MIP group was 43% lower than in the control (RR: 0.57, 95%CI: 0.33–1); however, this difference was statistically not significant. We observed the days on a vasopressor day (standardised mean difference [SMD]: 0.38; 95%CI: −1.20 to 0.44), length of ICU stay (SMD: 0.46; 95%CI: −1.44 to 0.51), secondary infection (RR: 0.75; 95%CI: 0.19–3.01), VAP (RR: 0.6; 95%CI: 0.28–1.56), CRBSI (RR: 0.97, 95%CI: 0.14–6.98), delta SOFA score (SMD: 0.88, 95%CI: −1.66 to − 0.10) between the two groups. Conclusions: Our findings observed preliminary evidence in the trends for a positive association of MIP with better outcomes in sepsis patients.

Abstract Background Little is known about the global distribution and environmental drivers of key microbial functional traits such as antibiotic resistance genes (ARGs). Soils are one of Earth’s largest reservoirs of ARGs, which are integral for soil microbial competition, and have potential implications for plant and human health. Yet, their diversity and global patterns remain poorly described. Here, we analyzed 285 ARGs in soils from 1012 sites across all continents and created the first global atlas with the distributions of topsoil ARGs. Results We show that ARGs peaked in high latitude cold and boreal forests. Climatic seasonality and mobile genetic elements, associated with the transmission of antibiotic resistance, were also key drivers of their global distribution. Dominant ARGs were mainly related to multidrug resistance genes and efflux pump machineries. We further pinpointed the global hotspots of the diversity and proportions of soil ARGs. Conclusions Together, our work provides the foundation for a better understanding of the ecology and global distribution of the environmental soil antibiotic resistome. Video Abstract

Abstract The purpose of the present paper is to examine the isometries of almost Ricci–Yamabe solitons. Firstly, the conditions under which a compact gradient almost Ricci–Yamabe soliton is isometric to Euclidean sphere $$S^n(r)$$ S n ( r ) are obtained. Moreover, we have shown that the potential f of a compact gradient almost Ricci–Yamabe soliton agrees with the Hodge–de Rham potential h. Next, we studied complete gradient almost Ricci–Yamabe soliton with $$\alpha \ne 0$$ α ≠ 0 and non-trivial conformal vector field with non-negative scalar curvature and proved that it is either isometric to Euclidean space $$E^n$$ E n or Euclidean sphere $$S^n.$$ S n . Also, solenoidal and torse-forming vector fields are considered. Lastly, some non-trivial examples are constructed to verify the obtained results.

Background: Road traffic accidents are as war on the roads and it takes the lives of approx 1.2 million individuals around the world. It is direct effect of modern day urban civilization. Occupied time between the accident and reaching the hospital is life-threatening and that is a very important gap to provide medical first aid to the victims. In urban life, 75% of thoracic trauma follows blunt injury. Greatest difficulty is faced in their management and long time in diagnosis. Hence, objective of study is to get knowledge of the blunt chest injuries among accident victims travelling in different modes of road transportation. Material and Methods: Data was collected from 100 cases of death by road traffic accident that were brought for medico-legal autopsy at the mortuary of Forensic Medicine Department, Rajrajeshwari Medical College and Hospital Bengaluru, during the period from December 2013 to May 2015. Results: Out of 100 cases studied, high risk of age of the victims were between 21-40 years (male 43.9% and female 44%). Males (82%) included the majority of victims as compared to females (18%) in the ratio 4.5:1. Most of the victims died on spot or within 6 hours of the incident (80%). Two-wheeler occupants (62%) were the commonest victims involved in RTA. Most of the RTA occurred in highway roads (78%). Most of patients succumbed on the way to hospital (54%). Conclusion: All blunt thoracic traumas constitute potential factor in increasing the amount of morbidity and mortality and therefore proper attention towards their accurate diagnosis and satisfactory management is mandatory. Last but not the least; a multidisciplinary approach is required for treating trauma victims.

Background: Problematic internet use (PIU) is a growing concern among teenage and college students. Excessive internet use has detrimental effects on physical and mental health. This study aims to assess the computer usage pattern and calculate the frequency of PIU in undergraduate health science students at Karnali Academy of Health Sciences, Jumla, a remote place of Nepal. Methods: A cross-sectional questionnaire-based study was conducted from October to November 2022. Data were collected from all the undergraduate students who consented to participate. The questionnaire included students’ computer-related behaviours. PIU was defined as a score of 42 or above on the PIU questionnaire. Data were analyzed by descriptive and analytical statistics, keeping p value

Abstract In wind energy, the general practice in assessing the wind resource of a site is to employ a 10‐min averaging to measured wind data. However, small wind turbines (SWTs) with rotor diameters

Abstract Introduction Harnessing synthetic communities (SynCom) of plant growth‐promoting (PGP) microorganisms is considered a promising approach to improve crop fitness and productivity. However, biotic mechanisms that underpin improved plant performance and the effects of delivery mode of synthetic community are poorly understood. These are critical knowledge gaps that constrain field efficacy of SynCom and hence large‐scale adoption by the farming community. Material & Methods In this study, a SynCom of four PGP microbial species was constructed and applied to either as seed dressing (treatment T1, applied at the time of sowing) or to soil (treatment T2, applied in soil at true leaf stage) across five different cotton (Gossypium hirsutum) cultivars. The impact of SynCom on plant growth, rhizosphere microbiome and soil nutrient availability, and how this was modified by plant variety and mode of applications, was assessed. Results Results showed that the seed application of SynCom had the strongest positive impact on overall plant fitness, resulting in higher germination (14.3%), increased plant height (7.4%) and shoot biomass (5.4%). A significant increase in the number of flowers (10.4%) and yield (8.5%) was also observed in T1. The soil nitrate availability was enhanced by 28% and 55% under T1 and T2, respectively. Results further suggested that SynCom applications triggered enrichment of members from bacterial phyla Actinobacteria, Firmicutes and Cyanobacteria in the rhizosphere. A shift in fungal communities was also observed, with a significant increase in the relative abundance of fungi from phyla Chytridiomycota and Basidiomycota in SynCom treatments. A structural equation model suggested that SynCom directly increased crop productivity but also indirectly via impacting the alpha diversity of bacteria. Conclusion Overall, this study provides mechanistic evidence that SynCom applications can shift rhizosphere microbial communities and improve soil fertility, plant growth, and crop productivity, suggesting that their use could contribute toward sustainable increase in farm productivity.

Abstract Background Estriol (E3) is a steroid hormone formed only during pregnancy in primates including humans. Although E3 is synthesized at large amounts through a complex pathway involving the fetus and placenta, it is not required for the maintenance of pregnancy and has classically been considered virtually inactive due to associated very weak canonical estrogen signaling. However, estrogen exposure during pregnancy may have an effect on organs both within and outside the reproductive system, and compounds with binding affinity for estrogen receptors weaker than E3 have been found to impact reproductive organs and the brain. Here, we explore potential effects of E3 on fetal development using mouse as a model system. Results We administered E3 to pregnant mice, exposing the fetus to E3. Adult females exposed to E3 in utero (E3-mice) had increased fertility and superior pregnancy outcomes. Female and male E3-mice showed decreased anxiety and increased exploratory behavior. The expression levels and DNA methylation patterns of multiple genes in the uteri and brains of E3-mice were distinct from controls. E3 promoted complexing of estrogen receptors with several DNA/histone modifiers and their binding to target genes. E3 functions by driving epigenetic change, mediated through epigenetic modifier interactions with estrogen receptors rather than through canonical nuclear transcriptional activation. Conclusions We identify an unexpected functional role for E3 in fetal reproductive system and brain. We further identify a novel mechanism of estrogen action, through recruitment of epigenetic modifiers to estrogen receptors and their target genes, which is not correlated with the traditional view of estrogen potency.

Abstract Introduction Plant‐associated microorganisms are widely explored for their use as bioinoculants in agriculture. However, the rate and ability of introduced microbes to colonise and interact with indigenous soil microbiomes are largely unknown. Materials & Methods In this study, we constructed a bacterial synthetic community (SynCom) using eight plant‐growth‐promoting bacteria isolated from the wheat (Triticum aestivum) rhizosphere, including three Bacillus spp., two Acinebacter spp., an Enterobacter sp., a Xanthomonas sp. and a Burkholderia sp., which all showed multiple plant growth‐promoting effects including indole‐3‐acetic acid and ammonia production and fungal pathogen suppression. We inoculated this SynCom in a soil with reduced microbial diversity, and investigated the ability of the SynCom to colonise wheat plants, and interact with soil microbes in the presence or absence of a soil‐borne pathogen Fusarium pseudograminearum (Fp). Results We found that SynCom significantly increased the wheat plant growth, root development and biomass production. Fp load in soil was significantly reduced and plant survival rates increased following the SynCom inoculation. Soil microbial community structure was altered by the SynCom, and noticeably, relative abundance of Pseudomonas spp. was induced in the soil. Conclusion This study provides novel evidence that colonisation of a beneficial SynCom promotes plant growth and alters soil microbial community.

Acidic soil is a serious harmful problem for rice crop productivity. Approximately 50% of the world’s potentially arable soils are acidic, whereas in North East (NE) India 80% of arable soils are effected. In nature, it exists synergistically with other metal stresses. Hence most of the studies to date were performed in combinations. This paper highlights the detrimental effect of acidity on plants to differentiate between the effect of acidity on plant growth to that of stress in combinations. We depict it through a cascade of morphological and physiological assays, including growth, reactive oxygen species (ROS), and photosynthesis-related parameters under acidic and non-acidic rhizospheric conditions in rice seedlings of Disang and Joymati. Up to 31% root length reduction was observed in Joymati, and up to 17% reduction in Disang variety; whereas, root-relative water content was observed to reduce by 3% in Disang and 9% was recorded in Joymati cultivars. Overall, we observed limited effect on morphometric parameters like root length, biomass, and chlorophyll content irrespective of variety analyzed. On the contrary, ROS accumulation was observed to be significantly increased; more in Joymati (sensitive variety) when compared to Disang (tolerant variety). Although there was not much decrease in chlorophyll content, photosynthesis was affected immensely as depicted from chlorophyll fluorescence parameters. Hence through this study, we hypothesize that the response of plants to acid stress is rather slow.

Background: Thrombocytopenia may result from mechanisms such as marrow hypoplasia, increased destruction of platelets, and splenic sequestration. The gold standard method for discriminating the causes of thrombocytopenia is bone marrow examination, but it is invasive and expensive. Therefore, an alternative method should be introduced as a first-line diagnostic procedure. Of late, the automated blood cell analyzer has made it possible to assess the cause of thrombocytopenia through various machine-derived parameters, known as platelet indices, which include the mean platelet volume (MPV), platelet distribution width (PDW), and plateletcrit (PCT), which are provided as a part of routine complete blood count. Objectives: The objectives of the present study are to study the variation and effectiveness of platelet indices in establishing the etiology of thrombocytopenia. Method: An observational, prospective, and comparative study was conducted on 134 patients with thrombocytopenia, and 67 cases were taken as the normal group. The study group was classified into two groups: hypo-productive and hyper-destructive. Platelet indices were recorded and compared in the two groups along with the normal group. Results: The mean platelet count (10^3 μL) in the normal, hypo-productive, and hyper-destructive groups was 232.03 ± 74.84, 73.00 ± 36.52, and 68.28 ± 38.24, respectively. The MPV and mean PCT in the normal, hypo-productive, and hyper-destructive groups were 9.46 ± 1.68fL, 8.99 ± 1.49fL, and 11.35 ± 1.35fL and 0.22 ± 0.06%, 0.07 ± 0.04%, and 0.08 ± 0.05%, respectively. The mean PDW in the normal, hypo-productive, and hyper-destructive groups was 15.66 ± 1.76fL, 17.63 ± 1.01fL, and 18.32 ± 1.10fL, respectively. Conclusion: In the present study, platelet indices such as MPV, PCT, and PDW are higher in the hyper-destructive group and may discriminate hyper-destructive from hypo-productive causes of thrombocytopenia.

Objective: Due to the lack of resources for culture and antibiotic susceptibility testing in most underdeveloped countries, puerperal sepsis is treated empirically with a wide range of antibiotics. Empirical treatment, on the other hand, does not ensure treatment effectiveness and may even contribute to antibiotic resistance. So, we studied cases of puerperal sepsis, its socio-demographic factors, bacterial isolates, and antibiotic sensitivity in a tertiary health center. Material and Methods: This was a cross-sectional study conducted at the obstetrics and gynecology department of a tertiary health center in India from April 2019 to September 2020. During this time, all patients with sepsis who met the criteria for inclusion were included. After granting an informed written consent, the subjects were registered on a pre-designed proforma. Results: There were 2,049 obstetrical admissions throughout this period, with 106 (5.1%) of these having puerperal sepsis. The majority of these women (58.7%) were between the ages of 21 and 30, were multiparous (96.5%), and unbooked. Fever 104 (98.1%) was the most prevalent clinical characteristic, whereas wound gape was the most common consequence (47.1%). Klebsiella aerogens was the most common organism found in various cultures. Many organisms were shown to be multidrug-resistant and sensitive to gentamycin and amikacin. Conclusion: Klebsiella aerogens wasthe most common cause of puerperal sepsis in this investigation. Because the causal agents of puerperal sepsis and their antibiotic sensitivity patterns change over time, positive blood culture and antibiotic susceptibility of the isolates are the best guides for selecting the optimum antimicrobial therapy for treating sepsis.

Background: Spinal anesthesia (SA) has many advantages in lower segment caesarean section (LSCS), but hypotension is the main issue that can be managed in different ways. This study was conducted to know the effectiveness of three intravenous serotonin receptor antagonists (ramosetron, ondansetron, and granisetron) in the prevention of bradycardia and hypotension during LSCS under SA. Patients and Methods: A total of 160 parturients posted for LSCS under SA were enrolled in this study and divided equally into four groups. Five minutes before SA, Group R received intravenous (IV) 0.3 mg ramosetron, Group O received 4 mg ondansetron IV, Group G received 1 mg granisetron IV, and Group S received IV normal saline. All solutions were diluted with normal saline to 5 mL to blind the drugs. Heart rate (HR), systolic and diastolic blood pressure, and mean arterial pressure were recorded. The total consumption of vasopressor and side effects were recorded. Results: The decrease in mean arterial pressure and mean HR was significantly lower in group R than other groups at all intervals (P < 0.05). The incidence of hypotension was 25% in group R compared with 42.5%, 55%, and 72.5% in groups O, G, and S, respectively. Conclusion: Premedication with intravenous ramosetron significantly reduced hypotension, HR, and total vasopressor usage in LSCS parturients. Intravenous ondansetron and granisetron had lesser effects than ramosetron.

Background: Behavioral management and carrying out surgical procedures for very young, uncooperative, anxious children can be challenging. It is usually encountered in children below the age of 6 years due to the following contributing factors such as immature reasoning, anxiety, fear, and inadequate coping skills. The goal of the study was to compare the efficacy of oral midazolam and oral triclofos sodium for conscious sedation in pediatric group of patients before elective surgeries. Materials and Methods: This prospective, single- blinded, randomized controlled, comparative study was conducted in 60 patients aged 2–8 years who were scheduled for elective surgery. The patients were randomly divided into two groups: M and T of 30 children each, where group M received 0.5 mg//kg midazolam and group T received 75 mg/kg triclofos sodium, orally, 60 min prior to anesthetic induction. The hemodynamics, acceptability of drugs, depth of sedation, and anxiety during separation from parents were assessed. Fisher’s exact test, chi-square test, and Student’s t test were applied to find significance. Results: The mean systolic and diastolic blood pressure, heart rate, respiration rate, and oxygen saturation at baseline to 60 min in groups M and T showed no significant difference between groups. Triclofos sodium has better acceptability over oral midazolam, whereas oral midazolam has better sedative properties over oral triclofos sodium. Conclusion: Oral midazolam was found to be more effective premedication and may be preferred over triclofos sodium due to the rapid and better sedative effect as well as good anxiolysis properties.

Anemia is the most common haematological disorder in pregnancy. Anemia increases maternal morbidity and mortality. It is a condition that can be diagnosed and treated during antenatal period, preventing the serious complications of anemia during pregnancy and labour. Aims: This study investigated the prevalence of types of anemia during pregnancy and fetomaternal outcomes among pregnant women. Study Design: This was a cross-sectional study that enrolled 1100 pregnant women who were in the third trimester of pregnancy with haemoglobin level

The rhizosphere microbes play a key role in plant nutrition and health. However, the interaction of beneficial microbes and Vigna unguiculata (lobia) production remains poorly understood. Thus, we aimed to isolate and characterize the soil microbes from the rhizosphere and develop novel microbial consortia for enhancing lobia production. Fifty bacterial strains were isolated from the rhizosphere soil samples of lobia. Finally, five effective strains (e.g., Pseudomonas sp. IESDJP-V1 and Pseudomonas sp. IESDJP-V2, Serratia marcescens IESDJP-V3, Bacillus cereus IESDJP-V4, Ochrobactrum sp. IESDJP-V5) were identified and molecularly characterized by 16 S rDNA gene amplification. All selected strains showed positive plant growth promoting (PGP) properties in broth culture. Based on morphological, biochemical, and plant growth promoting activities, five effective isolated strains and two collected strains (Azospirillum brasilense MTCC-4037 and Paenibacillus polymyxa BHUPSB17) were selected. The pot trials were conducted with seed inoculations of lobia (Vigna unguiculata) var. Kashi Kanchan with thirty treatments and three replications. The treatment combination T3 (Pseudomonas sp. IESDJP-V2), T14 (Pseudomonas sp. IESDJP-V2 + A. brasilense), T26 (Pseudomonas sp. IESDJP-V1+ B. cereus IESDJP-V4 + P. polymyxa) and T27 (IESDJP-V1+ IESDJP-V5+ A. brasilense) were recorded for enhancing plant growth attributes, yield, nutritional content like protein, total sugar, flavonoid and soil properties as compared to control and others. The effective treatments T3 (Pseudomonas sp.), T14 (Pseudomonas sp. IESDJP-V2 + A. brasilense), T26 (Pseudomonas sp. IESDJP-V1+ B. cereus IESDJP-V4 + P. polymyxa) and T27 (IESDJP-V1+ IESDJP-V5+ A. brasilense) recorded as potential PGPR consortium for lobia production. The treatment of single (Pseudomonas sp.), duel (IESDJP-V2 + A. brasilense) and triple combination (IESDJP-V1+ IESDJP-V4 + P. polymyxa) and (IESDJP-V1+ IESDJP-V5+ A. brasilense) can be further used for developing effective indigenous consortium for lobia production under sustainable farming practices. These PGPR bio-inoculant will be cost-effective, environment-friendly and socially acceptable.

Out of all emerging infectious diseases, approximately 75% are of zoonotic origin, with their source often traced back to animals. The emergence of zoonoses is driven by a complex interplay between anthropogenic, genetic, ecological, socioeconomic, and climatic factors. This intricate web of influences poses significant challenges for the prediction and prevention of zoonotic outbreaks. Effective coordination and collaboration among the animal, human, and environmental health sectors are essential for proactively addressing major zoonotic diseases. Despite advancements in surveillance and diagnostic practices, the emergence of zoonoses continues to be a pressing global concern. Therefore, prioritizing zoonotic disease surveillance is of paramount importance as part of a comprehensive disease prevention and containment strategy. Furthermore, evaluating existing surveillance systems provides insights into the challenges faced, which can be mitigated through implementation of One Health principles involving relevant stakeholders. To initiate multisectoral partnerships, it is crucial to identify the priorities and core themes of surveillance systems with equitable inputs from various sectors. Strengthening surveillance, promoting data sharing, enhancing laboratory testing capabilities, and fostering joint outbreak responses in both the human and animal health sectors will establish the necessary infrastructure to effectively prevent, predict, detect, and respond to emerging health threats, thereby reinforcing global health security. This review assesses existing surveillance approaches by offering an overview of global agencies engaged in monitoring zoonoses and outlines the essential components required at the human–animal–environment interface for designing comprehensive surveillance networks. Additionally, it discusses the key steps necessary for executing effective zoonotic disease surveillance through a One Health approach, while highlighting the key challenges encountered in establishing such a robust surveillance system.

Magnaporthe oryzae, the rice blast fungus, is one of the most dangerous rice pathogens, causing considerable crop losses around the world. In order to explore the rice blast-resistant sources, initially performed a large-scale screening of 277 rice accessions. In parallel with field evaluations, fifty-two rice accessions were genotyped for 25 major blast resistance genes utilizing functional/gene-based markers based on their reactivity against rice blast disease. According to the phenotypic examination, 29 (58%) and 22 (42%) entries were found to be highly resistant, 18 (36%) and 29 (57%) showed moderate resistance, and 05 (6%) and 01 (1%), respectively, were highly susceptible to leaf and neck blast. The genetic frequency of 25 major blast resistance genes ranged from 32 to 60%, with two genotypes having a maximum of 16 R-genes each. The 52 rice accessions were divided into two groups based on cluster and population structure analysis. The highly resistant and moderately resistant accessions are divided into different groups using the principal coordinate analysis. According to the analysis of molecular variance, the maximum diversity was found within the population, while the minimum diversity was found between the populations. Two markers (RM5647 and K39512), which correspond to the blast-resistant genes Pi36 and Pik, respectively, showed a significant association to the neck blast disease, whereas three markers (Pi2-i, Pita3, and k2167), which correspond to the blast-resistant genes Pi2, Pita/Pita2, and Pikm, respectively, showed a significant association to the leaf blast disease. The associated R-genes might be utilized in rice breeding programmes through marker-assisted breeding, and the identified resistant rice accessions could be used as prospective donors for the production of new resistant varieties in India and around the world.

Abstract Background The novel anti-malarial cipargamin (KAE609) has potent, rapid activity against Plasmodium falciparum. Transient asymptomatic liver function test elevations were previously observed in cipargamin-treated subjects in two trials: one in malaria patients in Asia and one in volunteers with experimentally induced malaria. In this study, the hepatic safety of cipargamin given as single doses of 10 to 150 mg and 10 to 50 mg once daily for 3 days was assessed. Efficacy results, frequency of treatment-emerging mutations in the atp4 gene and pharmacokinetics have been published elsewhere. Further, the R561H mutation in the k13 gene, which confers artemisinin-resistance, was associated with delayed parasite clearance following treatment with artemether–lumefantrine in Rwanda in this study. This was also the first study with cipargamin to be conducted in patients in sub-Saharan Africa. Methods This was a Phase II, multicentre, randomized, open-label, dose-escalation trial in adults with uncomplicated falciparum malaria in five sub-Saharan countries, using artemether–lumefantrine as control. The primary endpoint was ≥ 2 Common Terminology Criteria for Adverse Events (CTCAE) Grade increase from baseline in alanine aminotransferase (ALT) or aspartate transaminase (AST) during the 4-week trial. Results Overall, 2/135 patients treated with cipargamin had ≥ 2 CTCAE Grade increases from baseline in ALT or AST compared to 2/51 artemether–lumefantrine patients, with no significant difference between any cipargamin treatment group and the control group. Cipargamin exposure was comparable to or higher than those in previous studies. Hepatic adverse events and general safety and tolerability were similar for all cipargamin doses and artemether–lumefantrine. Cipargamin was well tolerated with no safety concerns. Conclusions This active-controlled, dose escalation study was a detailed assessment of the hepatic safety of cipargamin, across a wide range of doses, in patients with uncomplicated falciparum malaria. Comparison with previous cipargamin trials requires caution as no clear conclusion can be drawn as to whether hepatic safety and potential immunity to malaria would differ with ethnicity, patient age and or geography. Previous concerns regarding hepatic safety may have been confounded by factors including malaria itself, whether natural or experimental infection, and should not limit the further development of cipargamin. Trial registration ClinicalTrials.gov number: NCT03334747 (7 Nov 2017), other study ID CKAE609A2202

Rabbit rearing across the Indian subcontinent is growing rapidly. Growth traits are important parameters on which rabbits are being evaluated. Mathematical models have been tried to establish a relationship between body weight and time. Data spread over six years on the weekly body weight of Soviet Chinchilla and White Giant rabbits were collected and subjected to analysis for assessment of genetic parameters and factors which influence the weekly body weight. Three nonlinear growth models; Gompertz, Logistic, and Von Bertalanffy were fitted for both the breeds separately as well as collectively. The least squares mean for weekly body weights in rabbits from weaning to maturity showed an increasing trend. White Giant rabbits weigh higher than Soviet Chinchilla. The period and season of kindling had a significant influence on the weekly body weight of rabbits. The value of growth curve parameter k (an indicator of growth performance) was estimated to be higher for White Giant by all three models. All three models performed well with a high coefficient of determination values. However, Von Bertalanffy model fitted well for Soviet Chinchilla and Gompertz model for the White Giant as well as when both the breeds were fitted collectively.

Hepatitis C Virus (HCV) co-infection and its genotypic distribution in people living with Human Immunodeficiency Virus (HIV) show global inconsistency. Therefore, the present study aimed to investigate the prevalence and genotypic distribution patterns of HCV, along with viral load, in people living with HIV. This cross-sectional study was conducted at SRL Diagnostics Nepal, Pvt. Ltd. in 203 HIV-seropositive patients attending the Tribhuvan University Teaching Hospital (TUTH), Maharajgunj, Kathmandu, Nepal from October 2021 to May 2022. The viral load and HCV genotypes were estimated from RNA extracted from the blood sample (plasma) of PLHIV by using a standard Q-PCR protocol. HCV infection was considered as a core variable, whereas covariates used for this study were duration of HIV infection, age, sex, and ART regimen. Out of total 203 PLHIV, the estimated prevalence of HCV co-infection was 115 (56.6%). Male gender was a unique characteristic associated with a high prevalence of HCV co-infection compared to females. The HCV viral load among PLHIV ranged from 34 to 3,000,000 IU/mL. Among HCV co-infected PLHIV, 56 (48.69%) had a low level of HCV viral load. Interestingly, only 3 (2.6%) patients had an HCV viral load higher than 3,000,000 IU/mL. Diverse HCV genotypes were found in the population, including genotypes 1, 1a, 3a, 5a, and 6. However, genotype 3 was the most prevalent HCV variant among HCV-co-infected PLHIV, with a distribution of 36 (61.1%) and viral load ranging from 34 to 3000 IU/mL. HCV co-infection is frequent in the Nepalese population of people living with HIV, particularly due to HCV genotypic variant 3. The findings of this study could be useful for the management and clearance of the HCV co-infection in PLHIV, aiming to provide a good quality of life.

Plant hormones play an important role in growth, defence and plants productivity and there are several studies on their effects on plants. However, their role in humans and animals is limitedly studied. Recent studies suggest that plant hormone also works in mammalian systems, and have the potential to reduce human diseases such as cancer, diabetes, and also improve cell growth. Plant hormones such as indole-3-acetic acid (IAA) works as an antitumor, anti-cancer agent, gibberellins help in apoptosis, abscisic acid (ABA) as antidepressant compounds and regulation of glucose homeostasis whereas cytokinin works as an anti-ageing compound. The main aim of this review is to explore and correlate the relation of plant hormones and their important roles in animals, microbes and plants, and their interrelationships, emphasizing mainly human health. The most important and well-known plant hormones e.g., IAA, gibberellins, ABA, cytokinin and ethylene have been selected in this review to explore their effects on humans and animals.

Cyanobacteria have attracted the attention of researchers because of their promising role as primary and secondary metabolites in functional food and drug design. Due to an ever-increasing awareness of health and the use of natural products to avoid the onset of many chronic and lifestyle metabolic diseases, the global demand for the use of natural drugs and food additives has increased in the last few decades. There are several reports about the highly valuable cyanobacterial products such as carotenoids, vitamins, minerals, polysaccharides, and phycobiliproteins showing antioxidant, anti-cancerous, anti-inflammatory, hypoglycemic, and antimicrobial properties. Recently, it has been shown that allophycocyanin increases longevity and reduces the paralysis effect at least in Caenorhabditis elegans. Additionally, other pigments such as phycoerythrin and phycocyanin show antioxidative properties. Because of their high solubility in water and zero side effects, some of the cyanobacterial tetrapyrrole derivatives, i.e., pigments, facilitate an innovative and alternative way for the beverage and food industries in place of synthetic coloring agents at the commercial level. Thus, not only are the tetrapyrrole derivatives essential constituents for the synthesis of most of the basic physiological biomolecules, such as hemoglobin, chlorophyll, and cobalamin, but also have the potential to be used for the synthesis of synthetic compounds used in the pharmaceutical and nutraceutical industries. In the present review, we focused on the different aspects of tetrapyrrole rings in the drug design and food industries and addressed its remaining limitations to be used as natural nutrient supplements and therapeutic agents.

Abstract Background Cocoonase is a proteolytic enzyme that helps in dissolving the silk cocoon shell and exit of silk moth. Chemicals like anhydrous Na2CO3, Marseille soap, soda, ethylene diamine and tartaric acid-based degumming of silk cocoon shell have been in practice. During this process, solubility of sericin protein increased resulting in the release of sericin from the fibroin protein of the silk. However, this process diminishes natural color and softness of the silk. Cocoonase enzyme digests the sericin protein of silk at the anterior portion of the cocoon without disturbing the silk fibroin. However, no thorough characterization of cocoonase and sericin protein as well as imaging analysis of chemical- and enzyme-treated silk sheets has been carried out so far. Therefore, present study aimed for detailed characterization of cocoonase and sericin proteins, phylogenetic analysis, secondary and tertiary structure prediction, and computational validation as well as their interaction with other proteins. Further, identification of tasar silkworm (Antheraea mylitta) pupa stage for cocoonase collection, its purification and effect on silk sheet degumming, scanning electron microscope (SEM)-based comparison of chemical- and enzyme-treated cocoon sheets, and its optical coherence tomography (OCT)-based imaging analysis have been investigated. Various computational tools like Molecular Evolutionary Genetics Analysis (MEGA) X and Figtree, Iterative Threading Assembly Refinement (I-TASSER), self-optimized predicted method with alignment (SOPMA), PROCHECK, University of California, San Francisco (UCSF) Chimera, and Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) were used for characterization of cocoonase and sericin proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), protein purification using Sephadex G 25-column, degumming of cocoon sheet using cocoonase enzyme and chemical Na2CO3, and SEM and OCT analysis of degummed cocoon sheet were performed. Results Predicted normalized B-factors of cocoonase and sericin with respect to α and β regions showed that these regions are structurally more stable in cocoonase while less stable in sericin. Conserved domain analysis revealed that B. mori cocoonase contains a trypsin-like serine protease with active site range 45 to 180 query sequences while substrate binding site from 175 to 200 query sequences. SDS-PAGE analysis of cocoonase indicated its molecular weight of 25–26 kDa. Na2CO3 treatment showed more degumming effect (i.e., cocoon sheet weight loss) as compared to degumming with cocoonase. However, cocoonase-treated silk cocoon sheet holds the natural color of tasar silk, smoothness, and luster compared with the cocoon sheet treated with Na2CO3. SEM-based analysis showed the noticeable variation on the surface of silk fiber treated with cocoonase and Na2CO3. OCT analysis also exemplified the variations in the cross-sectional view of the cocoonase and Na2CO3-treated silk sheets. Conclusions Present study enlightens on the detailed characteristics of cocoonase and sericin proteins, comparative degumming activity, and image analysis of cocoonase enzyme and Na2CO3 chemical-treated silk sheets. Obtained findings illustrated about use of cocoonase enzyme in the degumming of silk cocoon at larger scale that will be a boon to the silk industry.

Abstract Passive radiative cooling is an emerging field and needs further development of material. Hence, the computational approach needs to establish for effective metamaterial design before fabrication. The finite difference time domain (FDTD) method is a promising numerical strategy to study electromagnetic interaction with the material. Here, we simulate using the FDTD method and report the behavior of various nanoparticles (SiO2, TiO2, Si3N4) and void dispersed polymers for the solar and thermal infrared spectrums. We propose the algorithm to simulate the surface emissive properties of various material nanostructures in both solar and thermal infrared spectrums, followed by cooling performance estimation. It is indeed found out that staggered and randomly distributed nanoparticle reflects efficiently in the solar radiation spectrum, become highly reflective for thin slab and emits efficiently in the atmospheric window (8–13 µm) over the parallel arrangement with slight variation. Higher slab thickness and concentration yield better reflectivity in the solar spectrum. SiO2-nanopores in a polymer, Si3N4 and TiO2 with/without voids in polymer efficiently achieve above 97% reflection in the solar spectrum and exhibits substrate independent radiative cooling properties. SiO2 and polymer combination alone is unable to reflect as desired in the solar spectrum and need a highly reflective substrate like silver.

First lactation production records of 7,825 Mehsana buffaloes, sired by 200 sires maintained at Dudhsagar Research and Development Association, Dudhsagar Dairy, Mehsana over a period of 25 years (1989-2013), were utilised for estimation of breeding value of the first lactation milk yield using BLUP - Univariate sire model and animal model using WOMBAT software. The study aimed to find out the optimum number of daughters that may be sufficient for more accurate and unbiased estimate of breeding value of Mehsana buffalo bulls. The effectiveness of different models run with different daughters per sire for sire evaluation was judged on the basis of within-sire variance. The maximum and minimum estimated breeding values were 1924.04 l (BLUP-AM, 5 daughters/sire) and 1855.61 l (BLUP-SM, 30 daughters/sire), respectively. However, error variance estimated was highest when five daughters/sire were evaluated and lowest, when 30 daughters/sire were evaluated. The present study revealed that the sire evaluation with sires having minimum 5, 10 and 15 daughters are equally efficient. However, if we do evaluation with sires having daughters more than 20, 25 or 30, additional accuracy of approximately 2.5%, 3% and 5%, respectively may be obtained.