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16 results on '"Jacquot, Aurore"'

4. Phosphoregulation in the N-terminus of NRT2.1 affects nitrate uptake by controlling the interaction of NRT2.1 with NAR2.1 and kinase HPCAL1 in Arabidopsis.

Author

Li, Zhi, Wu, Xu Na, Jacquot, Aurore, Chaput, Valentin, Adamo, Mattia, Neuhäuser, Benjamin, Straub, Tatsiana, Lejay, Laurence, and Schulze, Waltraud X

Subjects
NITRATES, ARABIDOPSIS
Abstract

NRT2.1, the major high affinity nitrate transporter in roots, can be phosphorylated at five different sites within the N- and the C-terminus. Here, we characterized the functional relationship of two N-terminal phosphorylation sites, S21 and S28, in Arabidopsis. Based on a site-specific correlation network, we identified a receptor kinase (HPCAL1, AT5G49770), phosphorylating NRT2.1 at S21 and resulting in active nitrate uptake. HPCAL1 itself was regulated by phosphorylation at S839 and S870 within its kinase domain. In the active state, when S839 was dephosphorylated and S870 was phosphorylated, HPCAL1 was found to interact with the N-terminus of NRT2.1, mainly when S28 was dephosphorylated. Phosphorylation of NRT2.1 at S21 resulted in a reduced interaction of NRT2.1 with its activator NAR2.1, but nitrate transport activity remained. By contrast, phosphorylated NRT2.1 at S28 enhanced the interaction with NAR2.1, but reduced the interaction with HPCAL1. Here we identified HPCAL1 as the kinase affecting this phospho-switch through phosphorylation of NRT2.1 at S21. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Plasma membrane calcineurin B‐like calcium‐ion sensor proteins function in regulating primary root growth and nitrate uptake by affecting global phosphorylation patterns and microdomain protein distribution

Author

Chu, Liang‐Cui, primary, Offenborn, Jan Niklas, additional, Steinhorst, Leonie, additional, Wu, Xu Na, additional, Xi, Lin, additional, Li, Zhi, additional, Jacquot, Aurore, additional, Lejay, Laurence, additional, Kudla, Jörg, additional, and Schulze, Waltraud X., additional

Published
2020
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8. Post-translational regulation of the root nitrate uptake transporter NRT2.1 in Arabidopsis thaliana

Author

Jacquot, Aurore, Chaput, Valentin, Mauries, Adeline, Li, Zhi, Bonillo, Pauline, Tillard, Pascal, Fizames, Cécile, Santoni-Kervella, Véronique, Gojon, Alain, Schulze, Waltraud, Lejay, Laurence, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), University of Hohenheim, Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

9. NRT2.1 phosphorylation prevents root high affinity nitrate uptake activity inArabidopsis thaliana

Author

Jacquot, Aurore, primary, Chaput, Valentin, additional, Mauries, Adeline, additional, Li, Zhi, additional, Tillard, Pascal, additional, Fizames, Cécile, additional, Bonillo, Pauline, additional, Bellegarde, Fanny, additional, Laugier, Edith, additional, Santoni, Véronique, additional, Hem, Sonia, additional, Martin, Antoine, additional, Gojon, Alain, additional, Schulze, Waltraud, additional, and Lejay, Laurence, additional

Published
2019
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10. Plasma membrane calcineurin B‐like calcium‐ion sensor proteins function in regulating primary root growth and nitrate uptake by affecting global phosphorylation patterns and microdomain protein distribution.

Author

Chu, Liang‐Cui, Offenborn, Jan Niklas, Steinhorst, Leonie, Wu, Xu Na, Xi, Lin, Li, Zhi, Jacquot, Aurore, Lejay, Laurence, Kudla, Jörg, and Schulze, Waltraud X.

Subjects
CELL membranes, ROOT growth, PHOSPHORYLATION, CALCINEURIN, MEMBRANE proteins
Abstract

Summary: The collective function of calcineurin B‐like (CBL) calcium ion (Ca2+) sensors and CBL‐interacting protein kinases (CIPKs) in decoding plasma‐membrane‐initiated Ca2+ signals to convey developmental and adaptive responses to fluctuating nitrate availability remained to be determined.Here, we generated a cbl‐quintuple mutant in Arabidopsis thaliana devoid of these Ca2+ sensors at the plasma membrane and performed comparative phenotyping, nitrate flux determination, phosphoproteome analyses, and studies of membrane domain protein distribution in response to low and high nitrate availability.We observed that CBL proteins exert multifaceted regulation of primary and lateral root growth and nitrate fluxes. Accordingly, we found that loss of plasma membrane Ca2+ sensor function simultaneously affected protein phosphorylation of numerous membrane proteins, including several nitrate transporters, proton pumps, and aquaporins, as well as their distribution within plasma membrane microdomains, and identified a specific phosphorylation and domain distribution pattern during distinct phases of low and high nitrate responses.Collectively, these analyses reveal a central and coordinative function of CBL–CIPK‐mediated signaling in conveying plant adaptation to fluctuating nitrate availability and identify a crucial role of Ca2+ signaling in regulating the composition and dynamics of plasma membrane microdomains. [ABSTRACT FROM AUTHOR]

Published
2021
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11. Post-translational regulation of the root nitrate uptake transporter NRT2.1 in A rabidopsis thaliana

Author

Jacquot, Aurore, Mauries, Adeline, LAUGIER, Edith, Hem, Sonia, Rofidal, Valerie, Tillard, Pascal, Santoni, Veronique, Schulze, Waltraud, Gojon, Alain, Lejay-Lefebvre, Laurence, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Plateforme de Spectrométrie de Masse Protéomique - Mass Spectrometry Proteomics Platform (MSPP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

12. Regulation of root nitrate uptake by carbon and nitrogen using NRT2.1 as a target

Author

Ruffel, Sandrine, Jacquot, Aurore, Fizames, Cécile, Shasha, Dennis, Tillard, Pascal, Santoni, Veronique, Schulze, W, Gojon, Alain, Guiterrez, Rodrigo, Lejay-Lefebvre, Laurence, Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), New York University [New York] (NYU), NYU System (NYU), FONDAP Center for Genome Regulation (CGR), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)

Subjects
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Abstract

absent

Published
2016

13. Coordinated Overexpression in Yeast of a P4-ATPase and Its Associated Cdc50 Subunit: The Case of the Drs2p/Cdc50p Lipid Flippase Complex

Author

Azouaoui, Hassina, Montigny, Cédric, Jacquot, Aurore, Barry, Raphaëlle, Champeil, Philippe, Lenoir, Guillaume, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de la Vision, Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Biochimie et Physiologie Moléculaire des Plantes ( BPMP ), Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, and Université de Montpellier (UM)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology, Saccharomyces cerevisiae Proteins, Membrane Proteins, Calcium-Transporting ATPases, Saccharomyces cerevisiae, P4-ATPase, atpase, Lipid transport, transport de lipides, Gene Expression Regulation, Enzymologic, Yeast, Co-expression, phosphorilation, Gene Expression Regulation, Fungal, Multiprotein Complexes, Cdc50 protein, Membrane protein, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Phosphorylation, levure, Phospholipids
Abstract

International audience; Structural and functional characterization of integral membrane proteins requires milligram amounts of purified sample. Unless the protein you are studying is abundant in native membranes, it will be critical to overexpress the protein of interest in a homologous or heterologous way, and in sufficient quantities for further purification. The situation may become even more complicated if you chose to investigate the structure and function of a complex of two or more membrane proteins. Here, we describe the overexpression of a yeast lipid flippase complex, namely the P4-ATPase Drs2p and its associated subunit Cdc50p, in a coordinated manner. Moreover, we can take advantage of the fact that P4-ATPases, like most other P-type ATPases, form an acid-stable phosphorylated intermediate, to verify that the expressed complex is functional.

Published
2015

14. High phosphatidylinositol 4-phosphate (PI4P)-dependent ATPase activity for the Drs2p-Cdc50p flippase after removal of its N- and C-terminal extensions

Author

Azouaoui, Hassina, primary, Montigny, Cédric, additional, Dieudonné, Thibaud, additional, Champeil, Philippe, additional, Jacquot, Aurore, additional, Vázquez-Ibar, José Luis, additional, Le Maréchal, Pierre, additional, Ulstrup, Jakob, additional, Ash, Miriam-Rose, additional, Lyons, Joseph A., additional, Nissen, Poul, additional, and Lenoir, Guillaume, additional

Published
2017
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15. A High-Yield Co-Expression System for the Purification of an Intact Drs2p-Cdc50p Lipid Flippase Complex, Critically Dependent on and Stabilized by Phosphatidylinositol-4-Phosphate

Author

Azouaoui, Hassina, primary, Montigny, Cédric, additional, Ash, Miriam-Rose, additional, Fijalkowski, Frank, additional, Jacquot, Aurore, additional, Grønberg, Christina, additional, López-Marqués, Rosa L., additional, Palmgren, Michael G., additional, Garrigos, Manuel, additional, le Maire, Marc, additional, Decottignies, Paulette, additional, Gourdon, Pontus, additional, Nissen, Poul, additional, Champeil, Philippe, additional, and Lenoir, Guillaume, additional

Published
2014
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