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This research study investigates the behaviors, knowledge, and skills necessary for academic library outreach work. Through a review of published literature, job advertisements, and a survey of library practitioners conducted in the fall of 2020, the authors define and prioritize 18 competencies for outreach. Hiring managers, LIS instructors, and practitioners can use the results of this study to structure and lay out the essential areas of outreach work in academic libraries.

This research study investigates the behaviors, knowledge, and skills necessary for academic library outreach work. Through a review of published literature, job advertisements, and a survey of library practitioners conducted in the fall of 2020, the authors define and prioritize 18 competencies for outreach. Hiring managers, LIS instructors, and practitioners can utilize the results of this study to structure and lay out the essential areas of outreach work in academic libraries.

This research study investigates the behaviors, knowledge, and skills necessary for academic library outreach work. Through a review of published literature, job advertisements, and a survey of library practitioners conducted in the fall of 2020, the authors define and prioritize 18 competencies for outreach. Hiring managers, LIS instructors, and practitioners can use the results of this study to structure and lay out the essential areas of outreach work in academic libraries. [ABSTRACT FROM AUTHOR]

1. Metabolic acidosis due to accumulation ofl-5-oxoproline is a rare, poorly understood, disorder associated with acetaminophen treatment in malnourished patients with chronic morbidity.l-5-Oxoprolinuria signals abnormal functioning of the γ-glutamyl cycle, which recycles and synthesises glutathione. Inhibition of glutathione synthetase (GS) by N-acetyl-p-benzoquinone imine (NAPQI) could contribute to 5-oxoprolinuric acidosis in such patients. We investigated the interaction of NAPQI with GSin vitro. 2. Peptide mapping of co-incubated NAPQI and GS using mass spectrometry demonstrated binding of NAPQI with cysteine-422 of GS, which is known to be essential for GS activity. Computational docking shows that NAPQI is properly positioned for covalent bonding with cysteine-422 via Michael addition and hence supports adduct formation. 3. Co-incubation of 0.77 μM of GS with NAPQI (25–400 μM) decreased enzyme activity by 16–89%. Inhibition correlated strongly with the concentration of NAPQI and was irreversible. 4. NAPQI binds covalently to GS causing irreversible enzyme inhibitionin vitro. This is an important novel biochemical observation. It is the first indication that NAPQI may inhibit glutathione synthesis, which is pivotal in NAPQI detoxification. Further studies are required to investigate its biological significance and its role in 5-oxoprolinuric acidosis. [ABSTRACT FROM AUTHOR]