1. Seminal plasma proteins and their relationship with percentage of morphologically normal sperm in 2-year-old Brahman (Bos indicus) bulls
- Author
-
Yutao Li, Michael McGowan, Gry Boe-Hansen, Arlindo A. Moura, J.P.A. Rego, B. Venus, J. M. Crisp, Amanda Nouwens, and Brian M. Burns
- Subjects
Male ,medicine.medical_specialty ,Apolipoprotein B ,Seminal Plasma Proteins ,Brahman ,Semen ,Biology ,Semen analysis ,Mass Spectrometry ,Endocrinology ,Food Animals ,Internal medicine ,medicine ,Animals ,Electrophoresis, Gel, Two-Dimensional ,Phosphoglycerate kinase 1 ,education ,education.field_of_study ,medicine.diagnostic_test ,General Medicine ,Blood proteins ,Spermatozoa ,Breed ,Semen Analysis ,biology.protein ,Animal Science and Zoology ,Cattle - Abstract
The objective was to determine the relationship between seminal plasma proteins and sperm morphology in Bos indicus bulls of the Brahman breed. Fifty-six 24-month-old Australian Brahman bulls were electroejaculated and samples were examined to determine the percentage of morphologically normal sperm (PNS24) and the seminal plasma protein composition was identified and quantified by 2-D gel electrophoresis. The total integrated optical density of 152 seminal plasma protein spots (SPPs) across all gels was determined using the PDQuest software version 8.0 (Bio Rad, USA). Using a single regression mixed model with the density of individual spots as a covariate for PNS24, 17 SPPs were significantly associated with PNS24 (p
- Published
- 2015