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Your search keyword '"Hirai, Nobumitsu"' showing total 46 results
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46 results on '"Hirai, Nobumitsu"'

4. AC Electromagnetic Field Controls the Biofilms on the Glass Surface by Escherichia coli & Staphylococcus epidermidis Inhibition Effect

Author

Aoyama, Natsu, primary, Kanematsu, Hideyuki, additional, Barry, Dana M., additional, Miura, Hidekazu, additional, Ogawa, Akiko, additional, Kogo, Takeshi, additional, Kawai, Risa, additional, Hagio, Takeshi, additional, Hirai, Nobumitsu, additional, Kato, Takehito, additional, Yoshitake, Michiko, additional, and Ichino, Ryoichi, additional

Published
2023
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18. Impedance Characteristics of Monolayer and Bilayer Graphene Films with Biofilm Formation and Growth

Author

Nakagawa, Ryoichi, primary, Saito, Kai, additional, Kanematsu, Hideyuki, additional, Miura, Hidekazu, additional, Ishihara, Masatou, additional, Barry, Dana M., additional, Kogo, Takeshi, additional, Ogawa, Akiko, additional, Hirai, Nobumitsu, additional, Hagio, Takeshi, additional, Ichino, Ryoichi, additional, Ban, Masahito, additional, Yoshitake, Michiko, additional, and Zimmermann, Stefan, additional

Published
2022
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21. Detection of Biofilm Formation on Material Surfaces by Ag + Coating.

Author

Kogo, Takeshi, Sugi, Kazufumi, Kanematsu, Hideyuki, Kai, Hotaka, Ogawa, Akiko, Hirai, Nobumitsu, Takahashi, Toshiyuki, and Kato, Takehito

Subjects
GENTIAN violet, SURFACES (Technology), ALKALI metal ions, BIOFILMS, IRON ions
Abstract

The evaluation of biofilm formation is important, given the ubiquity and problematic nature of biofilms in industrial and medical settings, as well as in everyday life. Basically, biofilms are formed on substrates. Therefore, it is essential to consider the properties of the substrates during biofilm evaluation. The common dye staining method to evaluate biofilm formation requires a short evaluation time and enables the evaluation of a large area of the sample. Furthermore, it can be easily determined visually, and quantitative evaluation is possible by quantifying color adsorption. Meanwhile, the dye staining method has the problem of adsorption even on substrate surfaces where no biofilm has formed. Therefore, in this study, we focused on Ag+ reduction reaction to devise a novel biofilm evaluation method. Ag+ is highly reductive and selectively reacts with organic substances, such as saccharides, aldehydes, and proteins contained in biofilms, depositing as metallic Ag. First, to simply evaluate biofilm formation, we used a glass substrate as a smooth, transparent, and versatile oxide material. We observed that the amount of Ag deposited on the substrate was increased proportionally to the amount of biofilm formed under light irradiation. Upon comparing the Ag deposition behavior and adsorption behavior of crystal violet, we discovered that for short immersion times in AgNO3 solution, Ag deposition was insufficient to evaluate the amount of biofilm formation. This result suggests that the Ag reduction reaction is more insensitive than the crystal violet adsorption behavior. The results of the Ag deposition reaction for 24 h showed a similar trend to the crystal violet dye adsorption behavior. However, quantitative biofilm evaluation using the proposed method was difficult because of the Ag+ exchange with the alkali metal ions contained in the glass substrate. We addressed this issue by using the basic solution obtained by adding an ammonia solution to aqueous AgNO3. This can cause Ag+ to selectively react with the biofilm, thus enabling a more accurate quantitative evaluation. The optimum was determined at a ratio of distilled water to aqueous ammonia solution of 97:3 by weight. This biofilm was also evaluated for materials other than ceramics (glass substrate): organic material (polyethylene) and metal material (pure iron). In the case of polyethylene, a suitable response and evaluation of biofilm formation was successfully achieved using this method. Meanwhile, in the case of pure iron, a significantly large lumpy deposit of Ag was observed. The likely reason is that Ag precipitation occurred along with the elution of iron ions because of the difference in ionization tendency. It could be concluded that the detection of biofilm formation using this method was effective to evaluate biofilm formation on materials, in which the reduction reaction of [Ag(NH3)2]+ does not occur. Thus, a simple and relatively quantitative evaluation of biofilms formed on substrates is possible using this method. [ABSTRACT FROM AUTHOR]

Published
2022
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26. Polymer Brush Made from Ionic Liquid and Its Anti-Biofilm Formation Behaviors By Environmental Biota in a Flow-Type Laboratory Biofilm Reactor

Author

Kanematsu, Hideyuki, primary, Oizumi, Atsuya, additional, Sato, Takaya, additional, Sato, Ryo, additional, Kamijo, Toshio, additional, Honma, Saika, additional, Hirai, Nobumitsu, additional, Kogo, Takeshi, additional, Ogawa, Akiko, additional, Tsunashima, Katsuhiko, additional, and Barry, Dana M., additional

Published
2020
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31. Biofilms By E.coli & S.Epidermidis and Its Sensing Possibility By Graphene-Dispersed Silane Coating

Author

Kanematsu, Hideyuki, primary, Nakagawa, Ryoichi, additional, Sano, Katsuhiko, additional, Barry, Dana M., additional, Itoh, Reo, additional, Yamada, Hirohisa, additional, Hirai, Nobumitsu, additional, Miura, Hidekazu, additional, Ogawa, Akiko, additional, Kogo, Takeshi, additional, Kuroda, Daisuke, additional, Tsunashima, Katsuhiko, additional, and Katakura, Katsumi, additional

Published
2019
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33. Biofilm Formation of a Polymer Brush Coating with Ionic Liquids Compared to a Polymer Brush Coating with a Non-Ionic Liquid

Author

Kanematsu, Hideyuki, primary, Oizumi, Atsuya, additional, Sato, Takaya, additional, Kamijo, Toshio, additional, Honma, Saika, additional, Barry, Dana, additional, Hirai, Nobumitsu, additional, Ogawa, Akiko, additional, Kogo, Takeshi, additional, Kuroda, Daisuke, additional, Sano, Katsuhiko, additional, Tsunashima, Katsuhiko, additional, Lee, Seung-Hyo, additional, and Lee, Myeong-Hoon, additional

Published
2018
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39. Biofilm Formation Behaviors on Graphene byE. coliandS. epidermidis

Author

Kanematsu, Hideyuki, primary, Sato, M, additional, Shindo, Kodai, additional, Barry, Dana M., additional, Hirai, Nobumitsu, additional, Ogawa, Akiko, additional, Kogo, Takeshi, additional, Utsumi, Yuichi, additional, Yamaguchi, Akinobu, additional, Ikegai, Hajime, additional, and Mizunoe, Yoshimitsu, additional

Published
2017
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45. Biofilm Formation Behaviors on Graphene by E. coli and S. epidermidis

Author

Kanematsu, Hideyuki, Sato, M, Shindo, Kodai, Barry, Dana M., Hirai, Nobumitsu, Ogawa, Akiko, Kogo, Takeshi, Utsumi, Yuichi, Yamaguchi, Akinobu, Ikegai, Hajime, and Mizunoe, Yoshimitsu

Abstract

The formation of biofilm on graphene materials was investigated in this study. Two kinds of laboratory biofilm reactors were used to produce biofilms artificially on glass specimens (on the laboratory scale). One of them was a loop-type and the biofilms were measured by Raman spectroscopy. The other one was the static immersion test in a culture containing bacteria. The biofilm formation was evaluated by using crystal violet. We expected that the graphene would have a strong resistance against biofilm formation. However, on the contrary, graphene tended to become more sensitive to biofilm formation when the portion of graphene increased. When we come to think about the future application for graphene, it should not be used for biomaterials, but as sensor for infectious diseases.

Published
2017

46. Biofilm Formation Behaviors on Graphene by E. coliand S. epidermidis

Author

Kanematsu, Hideyuki, Sato, M, Shindo, Kodai, Barry, Dana M., Hirai, Nobumitsu, Ogawa, Akiko, Kogo, Takeshi, Utsumi, Yuichi, Yamaguchi, Akinobu, Ikegai, Hajime, and Mizunoe, Yoshimitsu

Abstract

The formation of biofilm on graphene materials was investigated in this study. Two kinds of laboratory biofilm reactors were used to produce biofilms artificially on glass specimens (on the laboratory scale). One of them was a loop-type and the biofilms were measured by Raman spectroscopy. The other one was the static immersion test in a culture containing bacteria. The biofilm formation was evaluated by using crystal violet. We expected that the graphene would have a strong resistance against biofilm formation. However, on the contrary, graphene tended to become more sensitive to biofilm formation when the portion of graphene increased. When we come to think about the future application for graphene, it should not be used for biomaterials, but as sensor for infectious diseases.

Published
2017
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