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Earlier, we demonstrated the co-circulation of genetically distinct non-rodent-borne hantaviruses, including Boginia virus (BOGV) in the Eurasian water shrew ( Neomys fodiens ), Seewis virus (SWSV) in the Eurasian common shrew ( Sorex araneus ) and Nova virus (NVAV) in the European mole ( Talpa europaea ), in central Poland. To further investigate the phylogeny of hantaviruses harbored by soricid and talpid reservoir hosts, we analyzed RNAlater ® -preserved lung tissues from 320 shrews and 26 moles, both captured during 1990-2017 across Poland, and 10 European moles from Ukraine for hantavirus RNA through RT-PCR and DNA sequencing. SWSV and Altai virus (ALTV) were detected in Sorex araneus and Sorex minutus in Boginia and the Białowieża Forest, respectively, and NVAV was detected in Talpa europaea in Huta Dłutowska, Poland, and in Lviv, Ukraine. Phylogenetic analyses using maximum-likelihood and Bayesian methods showed geography-specific lineages of SWSV in Poland and elsewhere in Eurasia and of NVAV in Poland and Ukraine. The ATLV strain in Sorex minutus from the Białowieża Forest on the Polish-Belarusian border was distantly related to the ATLV strain previously reported in Sorex minutus from Chmiel in southeastern Poland. Overall, the gene phylogenies found support long-standing host-specific adaptation.

Warmer winters may lead to changes in the hibernation behaviour of bats, such as the barbastelle Barbastella barbastellus, which prefers to hibernate at low temperatures. The species is also known for its large annual fluctuations in the number of wintering individuals, so inference about population trends should be based on long-term data. Prior to 2005, analyses indicated stable or even increasing barbastelle population in Poland. We analysed the results of 13 winter bat counts (2005-2017) of the species from 15 of the largest hibernacula, and additional site of 47 small bunkers, in Poland. The total number of wintering individuals remained stable during the study period, because the barbastelle is not a long-distance migrant, this likely reflects the national population trend. On the basis of mean winter air temperatures we divided the country into four thermal regions. Analyses of barbastelle abundance in hibernacula in the four regions revealed a 4.8% annual mean increase in numbers in the coldest region, where mean winter temperatures were below -2°C, annual mean declines of 3.3% and 3.1% in two warmer regions of western Poland, but no trend in the region of intermediate mean winter temperatures of between -1°C and -2°C. Overall, there was a significant, but weak, negative correlation between the abundance of hibernating individuals and the mean winter temperature. On the other hand, the number of individuals hibernating in small bunkers increased, even though the site was located in one of the warm regions. The results indicate a warming climate will likely reduce the use of large, well-insulated winter roosts by species that prefer colder conditions-and that this is already happening. For forest-dwelling bats, such as the barbastelle, for which monitoring schemes are primarily based on winter surveys of large hibernacula, estimations of population trends may consequently become less reliable., Competing Interests: he authors have declared that no competing interests exist.

Animals living in anthropogenic habitats bear a multitude of costs, which are directly or indirectly associated with human activities. Among others, an elevated exposure to environmental pollution can have negative consequences for wildlife populations. We examined the differences in the concentrations of trace elements between the field and forest ecotype of the European roe deer (Capreolus capreolus). Naturally, roe deer inhabited various types of woodlands (forest ecotype), but within the last century, they adapted to life in a human-transformed agricultural areas (field ecotype), which could be associated with an increased exposure to pollution. In this study, we measured concentrations of seven trace metals (barium, copper, iron, lead, manganese, strontium, zinc) and fluoride in skull bones and permanent teeth of more than 230 roe deer from 8 study plots in East-Central Europe. We found that field roe deer had higher concentrations of four trace metals (copper, iron, lead, strontium) and fluoride compared with forest roe deer. These differences were consistent with variations in the general level of environmental contamination within the study plots, as assessed with trace element content in wild plants. Our study indicates that bone and teeth of the European roe deer can be used as a valid indicator of environmental pollution. Also, we expect that elevated exposure of field roe deer to environmental pollution can have negative consequences for wild populations of this species, as well as for the consumers of venison.

Game animals, such as the roe deer (Capreolus capreolus), have long been used as bioindicators of environmental contamination. Most ecotoxicological research on ungulates has focused on trace element content in soft tissues and antlers. Also, only fragmentary information exists about whether and how trace element concentrations vary with the age of wild-living animals and whether these age-related patterns are similar for different types of tissues. The purpose of this study was to measure concentrations of seven trace metals (barium, copper, iron, lead, manganese, strontium, zinc) and fluoride in bone and teeth of roe deer and to determine whether significant variation is evident with individual age. For this purpose, we collected permanent molars and fragments of mandible bone from more than 130 female roe deer in Central Poland. We found that concentrations of four trace elements (barium, manganese, zinc, and fluoride) in teeth of deer showed positive linear relationships with individual age. No such trends were recorded for trace element content in bone. We suggest that these striking differences in age-related patterns of trace element bioaccumulation between bone and permanent teeth of roe deer might be explained by higher turnover rate and constant remodelling of bone tissue. The results suggest that analysis of permanent teeth may be useful for assessing throughout-life intoxication by environmental pollution in the roe deer and possibly in other mammal species. Our study reinforces the need to carefully account for age-related variation in ecotoxicological research on wild-living animals.

Hantaviruses are zoonotic viruses with a complex evolutionary history of virus-host coevolution and cross-species transmission. Although hantaviruses have a broad reservoir host range, virus-host relationships were previously thought to be strict, with a single virus species infecting a single host species. Here, we describe Bruges virus, a novel hantavirus harbored by the European mole (Talpa europaea), which is the well-known host of Nova virus. Phylogenetic analyses of all three genomic segments showed tree topology inconsistencies, suggesting that Bruges virus has emerged from cross-species transmission and ancient reassortment events. A high number of coinfections with Bruges and Nova viruses was detected, but no evidence was found for reassortment between these two hantaviruses. These findings highlight the complexity of hantavirus evolution and the importance of further investigation of hantavirus-reservoir relationships., (© The Author 2017. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)

Copyright of [i2]: Research & Innovation in Architecture / Investigación e Innovación en Arquitectura y Territorio is the property of Universitat D'Alacant and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Genetically distinct hantaviruses have been identified in five species of fossorial moles (order Eulipotyphla, family Talpidae) from Eurasia and North America. Here, we report the isolation and partial characterization of a highly divergent hantavirus, named Nova virus (NVAV), from lung tissue of a European mole (Talpa europaea), captured in central Poland in August 2013. Typical hantavirus-like particles, measuring 80-120 nm in diameter, were found in NVAV-infected Vero E6 cells by transmission electron microscopy. Whole-genome sequences of the isolate, designated NVAV strain Te34, were identical to that amplified from the original lung tissue, and phylogenetic analysis of the full-length L, M and S segments, using maximum-likelihood and Bayesian methods, showed that NVAV was most closely related to hantaviruses harbored by insectivorous bats, consistent with an ancient evolutionary origin. Infant Swiss Webster mice, inoculated with NVAV by the intraperitoneal route, developed weight loss and hyperactivity, beginning at 16 days, followed by hind-limb paralysis and death. High NVAV RNA copies were detected in lung, liver, kidney, spleen and brain by quantitative real-time RT-PCR. Neuropathological examination showed astrocytic and microglial activation and neuronal loss. The first mole-borne hantavirus isolate will facilitate long-overdue studies on its infectivity and pathogenic potential in humans.

The complete genome sequence of Nova virus, a novel hantavirus isolated from a European mole (Talpa europaea) captured in central Poland, was determined. The availability of this sequence will facilitate the search for other mole-borne hantaviruses and will accelerate the acquisition of new knowledge about their phylogeography and evolutionary origin., (Copyright © 2015 Gu et al.)

Background: Rodent-borne orthohantaviruses are the causative agents of hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome. Apart from the classic rodent-borne hantaviruses, numerous species of hantaviruses have been identified in shrews and bats; however, their antigenicity and pathogenicity are unknown. This study focused on developing a serological method to detect antibodies against bat- and shrew-borne hantaviruses. Methodology/Principal findings: Five bat-borne (Brno, Dakrong, Quezon, Robina, and Xuan Song) and 6 shrew-borne (Asama, Altai, Cao Bang, Nova, Seewis, and Thottapalayam) viruses were selected based on the phylogenetic differences in their N proteins. The recombinant N (rN) proteins of these viruses were expressed as antigens in Vero E6 and 293T cell lines using the pCAGGS/MCS vector. Antisera against the Nus-tagged rN fusion proteins of these viruses (mouse anti-Brno, Dakrong, Quezon, Robina, Xuan Song, Asama, Cao Bang, and Nova, while rabbit anti-Altai, Seewis and Thottapalayam) were also generated. Antigenic cross-reactivity was examined in antisera and rN-expressing Vero E6 cells. The rN proteins of almost all the tested viruses, except for the Quezon and Robina viruses, showed independent antigenicity. For serological screening of bat samples, 5 rNs of the bat-borne viruses were expressed together in a single transfection protocol. Similarly, 6 rNs of shrew-borne viruses were expressed. Reactivities of the mixed antigen system were also examined across the singly transfected Vero cell lines to ensure that all antigens were expressed. Using these antigens, bat serum samples collected from Zambia were screened using the indirect immunofluorescence antibody test (IFAT). Selected positive samples were individually tested for the respective antigens by IFAT and western blot assays using rN-expressing 293T cell lysates. Of the 1,764 bat serum samples tested, 11.4% and 17.4% were positive for bat and shrew mixed antigens, respectively. These samples showed positive reactions to the Brno, Dakrong, Quezon, Xuan Son, Robina, Asama, Altai, Cao Bang, or Thottapalayam virus antigens. Conclusions/Significance: These observations suggest that the mixed-antigen screening system is useful for serological screening For Orthohantavirus infections and that bats in Zambia are likely exposed to not only bat-borne hantaviruses but also to shrew-borne hantaviruses. Author summary: Advancements in scientific research tools in areas of emerging and re-emerging zoonotic diseases of public health importance are crucial to understanding the epidemiology of these diseases. Hantaviruses are not an exception. Hantavirus pulmonary syndrome (HPS) and hemorrhagic fever with renal syndrome (HFRS) are the two main rodent-borne diseases of public health importance. However, bat- and shrew-borne hantaviruses have also been reported with serological detection of Thottapalayam and Altai shrew-borne hantavirus infections in humans in Sri Lanka and Thailand. Based on these recent public health discoveries, antigenic development and evaluation are part of the key components in efforts put forward to understand the epidemiology of these viruses. This study provides researchers with the first antigenic comparison between selected bat- and shrew-borne hantaviruses using their rN proteins with their application in screening epidemiological samples (Bat sera) from Zambia. Hence, this comprehensive study can serve as the basis for further scientific collaboration within research communities. [ABSTRACT FROM AUTHOR]

In this study, we used the depth-sensing indentation technique to determine the cracking resistance of different PVD hard coatings deposited on tool steel substrates. By comparison, with the load–displacement curves, measured at the sites of carbide inclusion and a tempered martensite matrix in the D2 tool steel substrate surface, we observed different fracture mechanisms on TiAlN hard coating prepared by sputtering. Additional information about the deformation and fracture phenomena was obtained from the SEM images of FIB cross-sections of both types of indents. We found that the main deformation mechanism in the coating is the shear sliding along the columnar boundaries, which causes the formation of steps on the substrate surface under individual columns. Using nanoindentation test, we also analyzed the cracking resistance of a set of nl-(Cr,Al)N nanolayer coatings with different Cr/Al atomic ratios, which were sputter deposited in a single batch. From the indentation curves, we determined the loads (Fc) at which the first pop-in appears and compared them with the plasticity index H3/E2. A good correlation of both parameters was found. We also compared the indentation curves of the TiAlN coating, which were prepared by cathodic arc evaporation using 1-fold, 2-fold and 3-fold rotation of the substrates. Additionally, on the same set of samples, the fracture toughness measurements were performed by micro-cantilever deflection test. The impact of growth defects on the cracking resistance of the hard coatings was also confirmed. [ABSTRACT FROM AUTHOR]

Simple Summary: In Central Europe, one of the main issues in wildlife management at present is its increasing abundance. As population density increases, diseases that directly or indirectly affect humans are also becoming more prevalent. Although fibropapillomatosis is one of the diseases that does not threaten humans, the accumulation of some heavy metals in tumours has been shown to occur at concentrations that would already be toxic to humans and wildlife. In general, the heavy metal content in wild animal tissues is a partly known topic that was studied on muscle or internal organs but is almost unstudied in tumours. Therefore, we evaluated the content of selected heavy metals in roe deer—the most widespread wildlife species across Europe. If the accumulation of these heavy metals in the tumours also affects the muscle of the individual, which is then intended for consumption by the final consumer, such meat could be considered a health hazard. This pilot study is a cornerstone for further research to clarify the safety or otherwise of meat from wild game affected by fibropapillomatosis. In recent decades, there has been an increase in European wild ungulate populations, often associated with a decline in health and spread of disease. This is true for the roe deer (Capreolus capreolus), the most common European cervid, with populations apparently affected by fibropapillomatosis, an increasingly common cancer. To date, however, there has been little research into this disease, thus many interactions remain unclear and descriptions of tumour composition are poorly validated. The main aim of the present study was to evaluate the presence and concentration of toxic heavy metals in roe deer skin tumours. Our results confirmed the presence of virtually all the metals tested for, i.e., Pb, Hg, Cd, As, Cr, Mn, Al, Co, Cu, Ni, Se, Zn, and Fe, with the highest average concentrations found for Cr (0.99 mg/kg−1 ± 2.23 SD), Cd (0.03 mg/kg−1 ± 0.03 SD), and Hg (0.02 mg/kg−1 ± 0.02 SD), exceeding FAO limits for meat from slaughtered animals. We also observed a significant positive relationship between heavy metal concentration and age, especially for Pb, As, Hg, Mn, Se, Al, Zn, and Ni. Our findings provide a strong baseline for further research on the impact of fibropapillomatosis, not only on the welfare and health status of game but also on the final consumer of venison, which in many respects is regarded as a high-quality, ecological, and renewable wild resource. While deer with this disease are not considered qualitatively or medically defective, they could represent a potential reservoir of substances toxic to humans and could affect substance levels in adjacent tissues or the animal as a whole. [ABSTRACT FROM AUTHOR]

Previously, we reported the discovery of a genetically distinct hantavirus, designated Boginia virus (BOGV), in the Eurasian water shrew (Neomys fodiens), as well as the detection of Seewis virus (SWSV) in the Eurasian common shrew (Sorex araneus), in central Poland. In this expanded study of 133 shrews and 69 moles captured during 2010-2013 in central and southeastern Poland, we demonstrate the co-circulation of BOGV in the Eurasian water shrew and SWSV in the Eurasian common shrew, Eurasian pygmy shrew (Sorex minutus) and Mediterranean water shrew (Neomys anomalus). In addition, we found high prevalence of Nova virus (NVAV) infection in the European mole (Talpa europaea), with evidence of NVAV RNA in heart, lung, liver, kidney, spleen and intestine. The nucleotide and amino acid sequence variation of the L segment among the SWSV strains was 0-18.8% and 0-5.4%, respectively. And for the 38 NVAV strains from European moles captured in Huta Dłutowska, the L-segment genetic similarity ranged from 94.1%-100% at the nucleotide level and 96.3%-100% at the amino acid level. Phylogenetic analyses showed geographic-specific lineages of SWSV and NVAV in Poland, not unlike that of rodent-borne hantaviruses, suggesting long-standing host-specific adaptation. The co-circulation and distribution of BOGV, SWSV and NVAV in Poland parallels findings of multiple hantavirus species co-existing in their respective rodent reservoir species elsewhere in Europe. Also, the detection of SWSV in three syntopic shrew species resembles spill over events observed among some rodent-borne hantaviruses., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Copyright of Arte, Individuo y Sociedad is the property of Universidad Complutense de Madrid and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Shrews (Soricidae) are common small wild mammals. Some species of shrews, such as Asian house shrews (Suncus murinus), have a significant overlap in their habitats with humans and domestic animals. Currently, over 190 species of viruses in 32 families, including Adenoviridae, Arenaviridae, Arteriviridae, Astroviridae, Anelloviridae, Bornaviridae, Caliciviridae, Chuviridae, Coronaviridae, Filoviridae, Flaviviridae, Hantaviridae, Hepadnaviridae, Hepeviridae, Nairoviridae, Nodaviridae, Orthoherpesviridae, Orthomyxoviridae, Paramyxoviridae, Parvoviridae, Phenuiviridae, Picobirnaviridae, Picornaviridae, Polyomaviridae, Poxviridae, Rhabdoviridae, Sedoreoviridae, Spinareoviridae, and three unclassified families, have been identified in shrews. Diverse shrew viruses, such as Borna disease virus 1, Langya virus, and severe fever with thrombocytopenia syndrome virus, cause diseases in humans and/or domestic animals, posing significant threats to public health and animal health. This review compiled fundamental information about shrews and provided a comprehensive summary of the viruses that have been detected in shrews, with the aim of facilitating a deep understanding of shrews and the diversity, epidemiology, and risks of their viruses. [ABSTRACT FROM AUTHOR]

The role of trace elements, heavy metals, and their effect on the development of hard tissue mineralization balance is poorly documented and the available results are often contradictory. The aim of the present study was the assessment of the concentration of the selected elements in the teeth of deer (Capreolus capreolus) and red deer (Cervus elaphus) with respect to their potential role in maintaining biomineralization balance in teeth hard tissues. Moreover, the aim was to determine whether trace elements accumulate in teeth with age. This study was conducted on 22 molar teeth of red deer (Cervus elaphus) and 54 molar teeth of roe deer (Capreolus capreolus) from Poland. Samples were analyzed with the use of inductively coupled plasma optical emission spectrometry equipped with a concentric nebulizer and cyclonic spray chamber in order to determine their Al, Ba, Cd, Fe, K, Li, Mg, Na, Ni, Pb, Sr, and Zn contents. There were strong positive correlations between strontium and zinc with heavy metals, particularly in dentine, which may affect the neutralization of the negative effects of heavy metals on tooth tissue and, consequently, maintaining ionic balance. The accumulation of trace elements with age in serenades has not been confirmed. [ABSTRACT FROM AUTHOR]

Copyright of Acta Universitatis Carolinae Philologica is the property of Charles University Prague, Karolinum Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of [i2]: Research & Innovation in Architecture / Investigación e Innovación en Arquitectura y Territorio is the property of Universitat D'Alacant and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The ionome—an important expression of the physiological state of organisms—is poorly known for mammals. The focus on particular tissues—such as liver, kidney, and bones—in biomonitoring of environmental pollution and potential deficiencies is based on widely held assumptions rather than solid knowledge of full mammalian ionomes. We examined the full ionome of Red deer (Cervus elaphus) and Wild boar (Sus scrofa), two commonly used mammals for biomonitoring, in a Dutch protected nature reserve (Veluwezoom). We used four individuals per species. We dissected 13 tissues and organs from each individuals (eight in total) of each species and measured 22 elemental concentrations in each. We assessed, for each element, how concentrations varied across tissues within and between individuals. Based on existing literature, we put our findings in the context of their function in the mammalian body. We found that the ionome was highly variable between as well as within the two species. For most elements, tissues containing the highest and lowest concentration differed between individuals. No single tissue accurately represented the accumulation of toxic elements or potential deficiencies in the bodies. Our assessment of the element's biological roles revealed a serious lack of reference values. Our findings imply that analyses of commonly used tissues in biomonitoring do not necessarily capture bioaccumulation of toxins or potential deficiencies. We recommend establishing a centralized database of mammalian ionomes to derive reference values in future. To our knowledge, our study is one of the most complete assessments of mammalian ionomes to date. [ABSTRACT FROM AUTHOR]

Results of a long-term study conducted in large bat hibernacula of central Europe showed upward trends in populations of many species over the last three decades. This study was aimed to check if similar changes in numbers could be observed in smaller underground winter roosts of bats. Surveys were made in three military shelters near Warsaw between 2000 and 2023 (once a year in February). These roosts hosted relatively small number of hibernating individuals (up to 38). Six bat species were recorded: Myotis nattereri, Myotis daubentonii, Eptesicus serotinus, Plecotus auritus, P. austriacus and Barbastella barbastellus. The species preferring cold sites for hibernation (P. auritus and B. barbastellus) predominated in the studied roosts, representing together over 86% of the recorded individuals. The numbers of the two most abundant species and of all bats showed statistically significant upward trends. [ABSTRACT FROM AUTHOR]

(1) In mammals, the mineral nutrient and trace elemental composition of the body - the ionome - differs among individuals. It has been hypothesized that these differences may be related to age and sex, both for ecotoxic and essential elements. (2) We investigated whether and how intraspecific ionomic variation is related to age and sex in Fallow deer (Dama dama). We tested the predictions that concentrations of ecotoxic elements increase with age, that ionomic variation is lower among young individuals than among older individuals, and that reproductive females (does) have the lowest concentrations of essential elements. (3) Culled animals of different sex and age were obtained from a single protected area. The animals were dissected to collect 13 tissues, and concentrations of 22 different elements were measured in a sample of each tissue. (4) We described substantial ionomic variation between individuals. Some of this variation was related to age and sex, as predicted. Based on the limited existing knowledge on chemical element allocation and metabolism in the body, sex-related differences were more difficult to interpret than age-related differences. Since reference values are absent, we could not judge about the consequences of the elemental values that we found. (5) More extensive ionomic surveys, based on a wide range of elements and tissues, are needed to enlarge the understanding of within-species ionomic variation and potential biological, ecological, and metabolic consequences. [ABSTRACT FROM AUTHOR]

The classical density topology is an extension of the natural topology on the real line, as the interior of arbitrary Lebesgue measurable set A is contained in the set of density points of A. Also each density point of A belongs to the closure of A for arbitrary measurable set A. In this paper, we concentrate on lower density operators for which the inclusions mentioned above are not fulfilled. In the first part, examples of such lower density operators generated by measure-preserving bijections are given. There are introduced three conditions to investigate lower density operators for which only the second inclusion holds. In the second part, the concept of operator D introduced by K. Kuratowski is applied to the characterization of such operators. [ABSTRACT FROM AUTHOR]

Woodland bat assemblages are usually structured in a space according to the distance from the ground, water, and obstacles, features that often define chiropteran hunting tactics. Consequently, the bat species composition differs strongly among various habitats, even within the same forest patch. However, when conducting local bat surveys in Wolin National Park (WPN), we revealed an unexpected uniformity in the qualitative and quantitative structure of bat assemblages, based on mist netting and ultrasound recording. In total, 10 vespertilionid species were detected. Across all methods and sampled habitats, a single species, Pipistrellus pygmaeus, predominated, while no Barbastella barbastellus, an old forest specialist, were detected, despite the abundance of their preferred daily roosts. We also reviewed the literature for mist-netted bat samples in four different habitats in lowland Polish forests. The samples usually clustered based on habitats, and the same habitat classes often clustered very closely despite representing geographically distant forests. The exception was WPN, where all four habitat classes formed a tightly packed cluster. We hypothesize that P. pygmaeus might act as a hyperabundant native species, a successful generalist that reduces the contribution of more specialized taxa in the assemblage. It probably benefits from both forest renaturation and anthropogenic cross-boundary subsidy. [ABSTRACT FROM AUTHOR]

Jiang and co-workers use high resolution STM to investigate the reaction and self-assembly of (3,6-dibromo-9,10-phenanthrenequinone, or DBPQ) molecules on Ag (100) and Ag (110) surfaces in order to understand the mechanism of bottom-up assembly on surfaces.[31] They show that, through the inclusion of multiple functional groups within a precursor molecule, it becomes possible to fabricate new low-dimensional materials with unique chemical, physical, and electronic properties. Herbst and Fransson consider the core-valence separation approximation that is often used in the calculation of core-level spectra.[5] They show how to quantify the errors in this approximation, thereby opening the door to error-quantified predictions relevant to x-ray spectroscopy. 153(16), 164108 (2020).10.1063/5.0019557 5 M. F. Herbst and T. Fransson, "Quantifying the error of the core-valence separation approximation", J. Chem. Phys. Zhu and co-workers tackle this problem for a model system containing a 2D semiconductor heterojunction and show convincingly the efficient hot electron transfer from photoexcited MoTe SB 2 sb to WS SB 2 sb .[30] This finding provides important insight into the competition between hot electron cooling and transfer at 2D semiconductor interfaces and suggests an intriguing possibility for the exploration of hot electron devices. [Extracted from the article]

Orthohantaviruses are mainly carried and transmitted by wild rodents, although during the last decade, they have also been identified in multiple species of shrews and moles. Orthohantavirus, Orthohantavirus seewisense (Seewis virus, SWSV), first detected in Switzerland in a single Sorex araneus (Eurasian common shrew) specimen, has been further described in several European countries, including Croatia's neighboring Slovenia and Hungary. Croatia is a well-known endemic region for several zoonotic agents including three different orthohantaviruses: Orthohantavirus puumalaense (PUUV), Orthohantavirus dobravaense (DOBV), and Orthohantavirus tulaense (TULV). In this study, nine shrews were tested and SWSV RNA was detected in liver, lung, and kidney belonging to two shrews (22.22%), one collected on Medvednica mountain in Zagreb County, and the other in the Stara Gradiška area in lowland Croatia. The phylogenetic analysis of the complete S segment's open reading frame (ORF) and partial L-segment revealed that the Croatian sequences, when compared to sequences from the adjacent geographic regions, form a specific genetic lineage. Two SWSV-positive shrew species—Sorex araneus and Neomys milleri (Mediterranean water shrew)—were identified using barcode-based sequence analysis. Therefore, the SWSV detection in N. milleri throughout the course of this study is seen as a rare find in this shrew species. To our knowledge, this is the first molecular and phylogenetic analysis of SWSV in Croatia. [ABSTRACT FROM AUTHOR]

In this paper, we prove some conditions for existence of an extension of a real (quasi-continuous) function with a closed graph defined on a given dense subset D of a topological space X to a (quasi-continuous) function with a closed graph on whole X. [ABSTRACT FROM AUTHOR]

Simple Summary: The rapidly growing antlers of farmed fallow deer (Dama dama) reflect the composition of their diet, and furthermore, the risk of toxic substances. The concentration of eight potentially toxic elements, PTE (Cd, Pb, As, Ba, Ni, Sr, La, Ce), was examined in the proximal, middle, and distal positions of the antlers and in the winter food and pasture food that the animals consumed during the increase in individual antler fragments depending on the age. The research was conducted on males aged 2 to 8 years. The oldest fallow deer had the highest amounts of As, Ba, and Sr in antlers. Increasing the body weight of animals and the weight of antlers resulted in a decrease in the concentration of Ba and Sr in tissues. The highest amounts of Cd were in food in June, and Ba in spring and winter. The obtained research results can be used by farmers to make seasonal decisions regarding nutrition. Breeders can produce food in areas with less pollution and harvest to prepare winter feed at a time when emissions and pollutions are lower. Deer antlers, usually harvested annually on a farm, are an accessible material used to determine the exposition to potentially toxic elements, PTEs, during growth. Moreover, the study of antlers from animals of different ages allows the assessment of long-term exposition to these elements. The aim of the study was to analyze the concentration of eight potentially toxic elements (Cd, Pb, As, Ba, Ni, Sr, La, Ce) in individual positions of the antlers (first, second, and third position, corresponding to the stages of development and life of these animals) and in the food that the animals consumed during the growth of individual antler fragments, depending on the age of the farmed fallow deer (Dama dama). The mineral composition of samples was analyzed using inductively coupled plasma mass spectrometry (ICP-MS). The analysis included 31 male deer aged 2–8 years old. The average concentration of Pb, Ba, and Ni was higher in the second position of the antler, and As, La, and Ce in the third position. In addition, the oldest individuals showed a higher Cd, Pb, and As concentration in the third position. A significant positive relationship was found between the age of animals and accumulation of As (r = 0.582, p < 0.05), as well as Ba and Sr (r = −0.534, r = −0.644 at p < 0.05, respectively). The average content of Ba and Sr also significantly negatively depended on body mass and antler mass stags (r = −0.436, r = −0.515 at p < 0.05, respectively). Cd concentration in feed was significantly higher in June compared to winter, spring, and later summer (p < 0.05). On the other hand, the concentration of Ba in food was significantly higher in spring and winter than in early and later summer (p < 0.05). An increase in the PTEs in the pasture determined the concentration of these components in fallow deer antlers. [ABSTRACT FROM AUTHOR]

When infecting humans, Andes orthohantavirus (ANDV) may cause a severe disease called hantavirus cardiopulmonary syndrome (HCPS). Following non-specific symptoms, the infection may progress to a syndrome of hemorrhagic fever combined with hyper-acute cardiopulmonary failure. The case fatality rate ranges between 25–40%, depending on the outbreak. In this study, we present the follow-up of a male patient who recovered from HCPS six years ago. We demonstrate that the ANDV genome persists within the reproductive tract for at least 71 months. Genome sequence analysis early and late after infection reveals a low number of mutations (two single nucleotide variants and one deletion), suggesting limited replication activity. We can exclude the integration of the viral genome into the host genome, since the treatment of the specimen with RNAse led to a loss of signal. We demonstrate a long-lasting, strong neutralizing antibody response using pseudovirions expressing the ANDV glycoprotein. Taken together, our results show that ANDV has the potential for sexual transmission. [ABSTRACT FROM AUTHOR]

Computational methods like molecular docking, pharmacokinetic study, molecular dynamic (MD) simulation and Molecular Mechanics‐Poisson‐Boltzmann Surface Area (MM‐PBSA) were used to investigate the ability of Glycyrrhiza glabra phytoconstituents to modulate the activity of GABA‐A receptor. The docking studies suggested that both Kanzonol U and Glabrol, have shown superior binding abilities, as evident by their binding energies of −11.8 and −11.4 kcal/mol, respectively, as compared to diazepam (−10.0 kcal/mol), which is an allosteric modulator of GABA‐A. Only nine constituents were identified as the blood‐brain barrier permeants in the SwissADME investigation, with binding energy≤−10.0 kcal/mol. The docking results were further strengthened by MD simulation which showed that the Kanzonol U and Glabrol complex displayed good conformational stability with an average RMSD of 0.20 nm. Additionally, MM‐PBSA outcomes revealed that these phytochemicals as the most potent GABA‐A modulators. All these investigations suggest that the phytochemicals Kanzonol U and Glabrol may produce a promising antiepileptic effect. [ABSTRACT FROM AUTHOR]

Purpose: The article aims to analyse the theoretical foundations of value management in both large enterprises and small and medium-sized businesses. Design/methodology/approach: The achievement of the objective involved the critical review of both national and international literature as the primary research method. Originality/value: Financial management, in theory and practice of a developed market economy is characterized by high dynamics, high level of evolution of the subject, scope and determinants of decisions made. At the same time, the basic goal of all enterprises is effective financial management as a determinant of the success or failure of an economic unit. When analyzing the financial management process, first of all, it is necessary to define the main goals, functions and management instruments, and then compare the company's goals with the goals of financial management. In the literature on the subject and in practice, you can meet many goals of the company's activity, i.e. maximizing profit, maximizing sales, achieving a satisfactory level of profit, survival of the company, but all these categories are directly or indirectly related to the profitability. However, the qualification of profit maximization as the superior goal of the enterprise raises a lot of controversy. On the other hand, profit maximization should not be treated as the goal of the company's activity, as it focuses on current effects, ignoring such an important issue as the structure of revenues over time. This gap is filled by maximizing the value of the owners' wealth. Undoubtedly, the main goal of the company is to maximize the financial benefits of the owners. Therefore, the question arises if this issue can be treated as the primary goal in the group of small and medium-sized enterprises. Each unit that conducts business activity must have at its disposal adequate capital which is the basis for the initiation and development of the company's activity. This capital can be obtained in many ways, but a significant part has to be contributed by co-owners. Of course, there are many groups of co-owners ranging from shareholders to sole proprietorship. Each shareholder expects to achieve specific benefits. Thus, he wants to be entitled to a share in the company's profits. The investment will be made in such a unit that will allow to obtain the greatest benefits, and therefore obtain the highest rate of return. [ABSTRACT FROM AUTHOR]

This paper, which serves as the introduction to the volume, suggests that stories of ancient Roman women shattering the curtain of silence that surrounds them can be repurposed to support contemporary women's struggle to make their voice heard. In particular, the tales of Io and Philomela in Ovid's Metamorphoses can be used to amplify the powerful message of the recently developed Me Too movement, as they show that women victim of sexual assault can reaffirm their identity and reclaim their role in the community by speaking up and denouncing the violence they have suffered. [ABSTRACT FROM AUTHOR]

Recombination of N2H+ ions with electrons was studied using a stationary afterglow with a cavity ring-down spectrometer. We probed in situ the time evolutions of number densities of different rotational and vibrational states of recombining N2H+ ions and determined the thermal recombination rate coefficients for N2H+ in the temperature range of 80–350 K. The newly calculated vibrational transition moments of N2H+ are used to explain the different values of recombination rate coefficients obtained in some of the previous studies. No statistically significant dependence of the measured recombination rate coefficient on the buffer gas number density was observed. [ABSTRACT FROM AUTHOR]

Hantaviridae currently encompasses seven genera and 53 species. Multiple hantaviruses such as Hantaan virus, Seoul virus, Dobrava-Belgrade virus, Puumala virus, Andes virus, and Sin Nombre virus are highly pathogenic to humans. They cause hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome or hantavirus pulmonary syndrome (HCPS/HPS) in many countries. Some hantaviruses infect wild or domestic animals without causing severe symptoms. Rodents, shrews, and bats are reservoirs of various mammalian hantaviruses. Recent years have witnessed significant advancements in the study of hantaviruses including genomics, taxonomy, evolution, replication, transmission, pathogenicity, control, and patient treatment. Additionally, new hantaviruses infecting bats, rodents, shrews, amphibians, and fish have been identified. This review compiles these advancements to aid researchers and the public in better recognizing this zoonotic virus family with global public health significance. [ABSTRACT FROM AUTHOR]

The recent detection of both Nova virus (NVAV) and Bruges virus (BRGV) in European moles (Talpa europaea) in Belgium and Germany prompted a search for related hantaviruses in the Iberian mole (Talpa occidentalis). RNAlater®-preserved lung tissue from 106 Iberian moles, collected during January 2011 to June 2014 in Asturias, Spain, were analyzed for hantavirus RNA by nested/hemi-nested RT-PCR. Pairwise alignment and comparison of partial L-segment sequences, detected in 11 Iberian moles from four parishes, indicated the circulation of genetically distinct hantaviruses. Phylogenetic analyses, using maximum-likelihood and Bayesian methods, demonstrated three distinct hantaviruses in Iberian moles: NVAV, BRGV, and a new hantavirus, designated Asturias virus (ASTV). Of the cDNA from seven infected moles processed for next generation sequencing using Illumina HiSeq1500, one produced viable contigs, spanning the S, M and L segments of ASTV. The original view that each hantavirus species is harbored by a single small-mammal host species is now known to be invalid. Host-switching or cross-species transmission events, as well as reassortment, have shaped the complex evolutionary history and phylogeography of hantaviruses such that some hantavirus species are hosted by multiple reservoir species, and conversely, some host species harbor more than one hantavirus species. [ABSTRACT FROM AUTHOR]

Hantaviruses are zoonotic rodent-borne viruses that are known to infect humans and cause various symptoms of disease, including hemorrhagic fever with renal and cardiopulmonary syndromes. They have a segmented single-stranded, enveloped, negative-sense RNA genome and are widely distributed. This study aimed to investigate the circulation of rodent-borne hantaviruses in peridomestic rodents and shrews in two semi-arid ecologies within the Kenyan Rift Valley. The small mammals were trapped using baited folding Sherman traps set within and around houses, then they were sedated and euthanatized through cervical dislocation before collecting blood and tissue samples (liver, kidney, spleen, and lungs). Tissue samples were screened with pan-hantavirus PCR primers, targeting the large genome segment (L) encoding the RNA-dependent RNA polymerase (RdRp). Eleven of the small mammals captured were shrews (11/489, 2.5%) and 478 (97.5%) were rodents. A cytochrome b gene-based genetic assay for shrew identification confirmed the eleven shrews sampled to be Crocidura somalica. Hantavirus RNA was detected in three (3/11, 27%) shrews from Baringo County. The sequences showed 93–97% nucleotide and 96–99% amino acid identities among each other, as well as 74–76% nucleotide and 79–83% amino acid identities to other shrew-borne hantaviruses, such as Tanganya virus (TNGV). The detected viruses formed a monophyletic clade with shrew-borne hantaviruses from other parts of Africa. To our knowledge, this constitutes the first report published on the circulation of hantaviruses in shrews in Kenya. [ABSTRACT FROM AUTHOR]

Hibernation is one of the most important behaviours of bats of the temperate zone. During winter, when little food or liquid water is available, hibernation in torpor lowers metabolic costs. However, the timing of emergence from hibernation is crucial for the resumption of the reproductive process in spring. Here, we investigate the spring emergence of six bat species or pairs of bat species of the genera Myotis and Plecotus at five hibernation sites in Central Europe over 5 years. Using generalized additive Poisson models (GAPMs), we analyze the influence of weather conditions (air and soil temperature, atmospheric pressure, atmospheric pressure trend, rain, wind, and cloud cover) as predictors of bat activity and separate these extrinsic triggers from residual motivation to emerge from hibernation (extrinsic factors not studied; intrinsic motivation). Although bats in a subterranean hibernaculum are more or less cut off from the outside world, all species showed weather dependence, albeit to varying degrees, with air temperature outside the hibernaculum having a significant positive effect in all species. The residual, potentially intrinsic motivation of species to emerge from their hibernacula corresponds to their general ecological adaptation, such as trophic specialization and roosting preferences. It allows the definition of three functional groups (high, medium and low residual activity groups) according to the degree of weather dependence of spring activity. A better knowledge of the interplay of extrinsic triggers and residual motivation (e.g., internal zeitgebers) for spring emergence will help to understand the flexibility of a species to adapt to a changing world. [ABSTRACT FROM AUTHOR]

Bat-associated hantaviruses have been detected in Asia, Africa and Europe. Recently, a novel hantavirus (Brno loanvirus, BRNV) was identified in common noctule bats (Nyctalus noctula) in the Czech Republic, but nothing is known about its geographical range and prevalence. The objective of this study was to evaluate the distribution and host specificity of BRNV by testing bats from neighbouring countries Germany, Austria and Poland. One thousand forty-seven bats representing 21 species from Germany, 464 bats representing 18 species from Austria and 77 bats representing 12 species from Poland were screened by L segment broad-spectrum nested reverse transcription-polymerase chain reaction (RT-PCR) or by BRNV-specific real-time RT-PCR. Three common noctules from Germany, one common noctule from Austria and three common noctules from Poland were positive in the hantavirus RNA screening. Conventional RT-PCR and primer walking resulted in the amplification of partial L segment and (almost) complete S and M segment coding sequences for samples from Germany and partial L segment sequences for samples from Poland. Phylogenetic analysis of these nucleotide sequences showed highest similarity to BRNV from Czech Republic. The exclusive detection of BRNV in common noctules from different countries suggests high host specificity. The RNA detection rate in common noctules ranged between 1 of 207 (0.5%; Austria), 3 of 245 (1.2%; Germany) and 3 of 20 (15%; Poland). In conclusion, this study demonstrates a broader distribution of BRNV in common noctules in Central Europe, but at low to moderate prevalence. Additional studies are needed to prove the zoonotic potential of this hantavirus and evaluate its transmission within bat populations. [ABSTRACT FROM AUTHOR]

The aim of this study was to compare the chemical composition and physicochemical and sensory properties of meat (m. longissimus lumborum, LL) from wild-living female red deer (Cervus elaphus L.) aged 4-6 years (15 animals) and female roe deer (Capreolus capreolus L.) aged 3-5 years (20 animals). The animals were hunter-harvested in the forests of north-eastern Poland. The LL muscle of roe deer had a higher (P ≤ 0.05) content of moisture, total protein and fat and a higher (P ≤ 0.05) pH value. In the case of red deer, the LL muscle had a higher (P ≤ 0.05) contribution of redness and yellowness, a more intense (P ≤ 0.05) taste, and higher (P ≤ 0.05) juiciness scores. The quality of meat from both deer species was very high, and neither can be considered superior in that respect based on the differences noted. [ABSTRACT FROM AUTHOR]

Temperature measurements in biological tissues play a crucial role in studying metabolic activities. In this study, we introduce a noninvasive thermometry technique based on two-color ultrasound-switchable fluorescence (USF). This innovative method allows for a local temperature mapping within a microtube filled with temperature-sensitive liposomes as nano imaging agents. By measuring the temperature-dependent fluorescence emission of the liposomes using a spectrometer, we identify four characteristic temperatures. The local background temperature can be estimated by analyzing the corresponding appearance time of these four characteristic temperatures in the dynamic USF signals captured by a camera-based USF system with two detection channels. Simultaneous measurements with an infrared (IR) camera showed a 0.38°C ± 0.27°C difference between USF thermometry and IR thermography in a physiological temperature range of 36.48°C-40.14°C. This shows that the two-color USF thermometry technique is a reliable, noninvasive tool with excellent spatial and thermal resolution., (© 2024 Wiley‐VCH GmbH.)

This paper considers misunderstandings found within the story of Cephalus and Procris in Metamorphoses 7, misunderstandings that are regularly repeated in the scholarly interpretations and English translations of this episode. The interpretive challenges posed by Cephalus’ tale are indeed akin to those faced by the Ovidian translator, whose interpretive choices will influence how later readers construe his often ambiguous words. Whereas others see Cephalus as an unreliable narrator whose claim to have been raped by Aurora is open to doubt, I argue that his words are best read as he tells us to read them. Misunderstanding is due not to Cephalus’ unreliability but to the interpretive challenges posed by the changeable Ovidian world he inhabits, a world that is labyrinthine and ambiguous. Ovid himself helps us navigate the mazelike structure of Cephalus’ narrative by verbal clues and intra-/intertextual parallels that clearly align Cephalus with other rape victims in the epic. By understanding Cephalus first and foremost as a rape victim, his actions and words become more intelligible and shed light on the themes of rape, masculinity, and power within the epic as a whole. [ABSTRACT FROM AUTHOR]

The official classification of newly discovered or long-known unassigned viruses by the International Committee on Taxonomy of Viruses (ICTV) requires the deposition of coding-complete or -near-complete virus genome sequences in GenBank to fulfill a requirement of the taxonomic proposal (TaxoProp) process. However, this requirement is fairly new; thus, genomic sequence information is fragmented or absent for many already-classified viruses. As a result, taxon-wide modern phylogenetic analyses are often challenging, if not impossible. This problem is particularly eminent among viruses with segmented genomes, such as bunyavirals, which were frequently classified solely based on single-segment sequence information. To solve this issue for one bunyaviral family, Hantaviridae, we call on the community to provide additional sequence information for incompletely sequenced classified viruses by mid-June 2023. Such sequence information may be sufficient to prevent their possible declassification during the ongoing efforts to establish a coherent, consistent, and evolution-based hantavirid taxonomy. [ABSTRACT FROM AUTHOR]

Copyright of Tasarim+Kuram is the property of KARE Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We prove that the lower density operator associated with the Baire category density points in the real line has Borel values of class Π 3 0 which is analogous to the measure case. We also introduce the notion of the Baire category density point of a subset with the Baire property of the Cantor space, and we prove that it generates a lower density operator with Borel values of class Π 3 0 . [ABSTRACT FROM AUTHOR]

Copyright of Journal of Architectural Sciences & Applications (JASA) is the property of Journal of Architectural Sciences & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)