233 results on '"H. Sugimura"'
Search Results
2. Commissioning Runs of J-PARC E16 Experiment
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M. Ichikawa, K. Aoki, D. Arimizu, S. Ashikaga, W.-C. Chang, T. Chujo, K. Ebata, H. En’yo, S. Esumi, H. Hamagaki, R. Honda, S. Kajikawa, K. Kanno, Y. Kimura, A. Kiyomichi, T.K. Kondo, S. Kyan, C.-H. Lin, C.-S. Lin, Y. Morino, H. Murakami, T.N. Murakami, R. Muto, W. Nakai, S. Nakasuga, M. Naruki, T. Nonaka, H. Noumi, K. Ozawa, T. Sakaguchi, H. Sako, F. Sakuma, S. Sato, S. Sawada, M. Sekimoto, K. Shigaki, K. Shirotori, H. Sugimura, T.N. Takahashi, Y. Takaura, R. Tatsumi, K. Tsukui, P.-H. Wang, K. Yahiro, K.H. Yamaguchi, and S. Yokkaichi
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General Physics and Astronomy - Published
- 2023
3. SuperKEKB operation using crab waist collision scheme
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Xiangyu Zhou, S. Nakamura, Michiru Nishiwaki, Takashi Mori, Toshiyuki Oki, Katsunobu Oide, H. Sugimura, Hiroshi Kaji, Masafumi Tawada, Hitomi Ikeda, Toshihiro Mimashi, R. Ueki, Yusuke Suetsugu, Shinji Terui, Demin Zhou, S. Enomoto, Yasushi Arimoto, Haruyo Koiso, G. Mitsuka, Zhanguo Zong, T. Miyajima, R. Yang, Akio Morita, Xudong Wang, Tatsuro Nakamura, Yoshihiro Funakoshi, Kyo Shibata, Kazuro Furukawa, Mitsuo Kikuchi, Kazuhito Ohmi, T. Yoshimoto, Kota Kodama, Hitoshi Fukuma, S. Yoshimoto, Hiroshi Sugimoto, Ken Watanabe, Takuya Kamitani, M. A. Rehman, Kazunori Akai, T. Kobayashi, Kazumi Egawa, Kota Nakanishi, Tetsuo Abe, Takuya Ishibashi, S. Ogasawara, Norihito Ohuchi, Mika Masuzawa, Yukiyoshi Ohnishi, Yuji Seimiya, Makoto Tobiyama, Fusashi Miyahara, T. Kawamoto, Naoko Iida, and S. Iwabuchi
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Scheme (programming language) ,Physics ,Low emittance ,Waist ,Luminosity (scattering theory) ,Interaction point ,Astrophysics::High Energy Astrophysical Phenomena ,General Physics and Astronomy ,Collision ,law.invention ,Nuclear physics ,symbols.namesake ,law ,symbols ,Physics::Accelerator Physics ,High Energy Physics::Experiment ,Collider ,computer ,Beta function ,computer.programming_language - Abstract
SuperKEKB is an electron–positron asymmetric-energy double-ring collider, which was built in Japan. It has been operated to explore new phenomena in B-meson decays. Hence, extremely higher luminosity is required. A collision scheme of low emittance with a large Piwinski angle called a “nano-beam scheme” has been adopted to achieve higher luminosity by squeezing the vertical beta function at the interaction point to be smaller than the bunch length. A “crab waist collision scheme” proposed by P. Raimondi et al. has also been adopted to improve the luminosity performance. The article presents an overview of the operation of the nano-beam and crab waist collision schemes at SuperKEKB.
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- 2021
4. Dietary magnesium intake and risk of incident coronary heart disease in men: A prospective cohort study
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Yoshihiro Kokubo, Isao Saito, Hiroyasu Iso, Kazumasa Yamagishi, Hiroshi Yatsuya, Junko Ishihara, Koutatsu Maruyama, Manami Inoue, Norie Sawada, Shoichiro Tsugane, S. Tsugane, N. Sawada, M. Iwasaki, S. Sasazuki, T. Yamaji, T. Shimazu, T. Hanaoka, J. Ogata, S. Baba, T. Mannami, A. Okayama, Y. Kokubo, K. Miyakawa, F. Saito, A. Koizumi, Y. Sano, I. Hashimoto, T. Ikuta, Y. Tanaba, H. Sato, Y. Roppongi, T. Takashima, H. Suzuki, Y. Miyajima, N. Suzuki, S. Nagasawa, Y. Furusugi, N. Nagai, Y. Ito, S. Komatsu, T. Minamizono, H. Sanada, Y. Hatayama, F. Kobayashi, H. Uchino, Y. Shirai, T. Kondo, R. Sasaki, Y. Watanabe, Y. Miyagawa, Y. Kobayashi, M. Machida, K. Kobayashi, M. Tsukada, Y. Kishimoto, E. Takara, T. Fukuyama, M. Kinjo, M. Irei, H. Sakiyama, K. Imoto, H. Yazawa, T. Seo, A. Seiko, F. Ito, F. Shoji, R. Saito, A. Murata, K. Minato, K. Motegi, T. Fujieda, S. Yamato, K. Matsui, T. Abe, M. Katagiri, M. Suzuki, M. Doi, A. Terao, Y. Ishikawa, T. Tagami, H. Sueta, H. Doi, M. Urata, N. Okamoto, F. Ide, H. Goto, R. Fujita, N. Onga, H. Takaesu, M. Uehara, T. Nakasone, M. Yamakawa, F. Horii, I. Asano, H. Yamaguchi, K. Aoki, S. Maruyama, M. Ichii, M. Takano, Y. Tsubono, K. Suzuki, Y. Honda, K. Yamagishi, S. Sakurai, N. Tsuchiya, M. Kabuto, M. Yamaguchi, Y. Matsumura, S. Sasaki, S. Watanabe, M. Akabane, T. Kadowaki, M. Inoue, M. Noda, T. Mizoue, Y. Kawaguchi, Y. Takashima, Y. Yoshida, K. Nakamura, R. Takachi, J. Ishihara, S. Matsushima, S. Natsukawa, H. Shimizu, H. Sugimura, S. Tominaga, N. Hamajima, H. Iso, T. Sobue, M. Iida, W. Ajiki, A. Ioka, S. Sato, E. Maruyama, M. Konishi, K. Okada, I. Saito, N. Yasuda, S. Kono, S. Akiba, and T. Isobe
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Male ,medicine.medical_specialty ,Coronary Disease ,Disease ,030204 cardiovascular system & hematology ,Critical Care and Intensive Care Medicine ,Dietary Magnesium ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Risk Factors ,Surveys and Questionnaires ,Internal medicine ,Humans ,Medicine ,Magnesium ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Stroke ,Aged ,Nutrition and Dietetics ,business.industry ,Hazard ratio ,Middle Aged ,medicine.disease ,Diet Records ,Coronary heart disease ,Confidence interval ,Diet ,Physical therapy ,Population study ,Female ,business - Abstract
Summary Background & aims The associations between dietary magnesium intake and stroke and coronary heart disease (CHD) incidences are inconsistent and not established in Asian. We aimed to determine the association between dietary magnesium intake and the risk of stroke and CHD in a Japanese population. Subjects/Methods We studied 85,293 Japanese subjects by questionnaire at baseline (age 45–74 years, without cardiovascular disease or cancer in 1995 and 1998 for Cohorts I and II, respectively). The participants were followed until the end of 2009 and 2010 in Cohorts I and II, respectively. Dietary magnesium intake was estimated from a self-administered 138-item food-frequency questionnaire. Results After 1,305,738 person-years of follow-up, 4110 strokes and 1283 cases of CHD were documented. The multivariable-adjusted hazard ratios (HRs, 95% confidence intervals, 95%CIs) of CHD for the fourth and fifth quintiles of dietary magnesium intake were 0.70 (0.50–0.99) and 0.66 (0.44–0.97) in men (P for trend = 0.036), respectively, and third quintile of dietary magnesium intake was 0.61 (0.39–0.96) in women (P for trend = 0.241), compared with the lowest quintile in men and women. We observed no decreased risks of incident stroke in men or women with higher dietary magnesium intakes. Conclusions Higher dietary magnesium intake was associated with a reduced risk of CHD in Japanese men.
- Published
- 2018
5. Observation of Coulomb-Assisted Nuclear Bound State of Ξ^{-}-^{14}N System
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S H, Hayakawa, K, Agari, J K, Ahn, T, Akaishi, Y, Akazawa, S, Ashikaga, B, Bassalleck, S, Bleser, H, Ekawa, Y, Endo, Y, Fujikawa, N, Fujioka, M, Fujita, R, Goto, Y, Han, S, Hasegawa, T, Hashimoto, T, Hayakawa, E, Hayata, K, Hicks, E, Hirose, M, Hirose, R, Honda, K, Hoshino, S, Hoshino, K, Hosomi, S H, Hwang, Y, Ichikawa, M, Ichikawa, K, Imai, K, Inaba, Y, Ishikawa, H, Ito, K, Ito, W S, Jung, S, Kanatsuki, H, Kanauchi, A, Kasagi, T, Kawai, M H, Kim, S H, Kim, S, Kinbara, R, Kiuchi, H, Kobayashi, K, Kobayashi, T, Koike, A, Koshikawa, J Y, Lee, T L, Ma, S Y, Matsumoto, M, Minakawa, K, Miwa, A T, Moe, T J, Moon, M, Moritsu, Y, Nagase, Y, Nakada, M, Nakagawa, D, Nakashima, K, Nakazawa, T, Nanamura, M, Naruki, A N L, Nyaw, Y, Ogura, M, Ohashi, K, Oue, S, Ozawa, J, Pochodzalla, S Y, Ryu, H, Sako, S, Sato, Y, Sato, F, Schupp, K, Shirotori, M M, Soe, M K, Soe, J Y, Sohn, H, Sugimura, K N, Suzuki, H, Takahashi, T, Takahashi, T, Takeda, H, Tamura, K, Tanida, A M M, Theint, K T, Tint, Y, Toyama, M, Ukai, E, Umezaki, T, Watabe, K, Watanabe, T O, Yamamoto, S B, Yang, C S, Yoon, J, Yoshida, M, Yoshimoto, D H, Zhang, and Z, Zhang
- Abstract
In an emulsion-counter hybrid experiment performed at J-PARC, a Ξ^{-} absorption event was observed which decayed into twin single-Λ hypernuclei. Kinematic calculations enabled a unique identification of the reaction process as Ξ^{-}+^{14}N→_{Λ}^{10}Be+_{Λ}^{5}He. For the binding energy of the Ξ^{-} hyperon in the Ξ^{-}-^{14}N system a value of 1.27±0.21 MeV was deduced. The energy level of Ξ^{-} is likely a nuclear 1p state which indicates a weak ΞN-ΛΛ coupling.
- Published
- 2020
6. Dietary patterns and colorectal cancer risk in middle-aged adults: A large population-based prospective cohort study
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M. Ichii, S. Sakurai, Yoshihiro Kokubo, Y. Tsubono, N. Suzuki, H. Goto, T. Kondo, Y. Sato, Takashi Fujieda, Hiroyasu Iso, K. Aoki, M. Doi, T. Isobe, M. Kinjo, Kouji Minato, Norie Sawada, K. Imoto, H. Suzuki, E. Takara, Y. Watanabe, S. Tominaga, R. Sasaki, S. Sato, T. Abe, Y. Ito, Y. Roppongi, T. Tagami, Y. Kishimoto, M. Iwasaki, Y. Miyajima, K. Nakamura, T. Seo, S. Komatsu, Minoru Iida, S. Matsushima, Taiki Yamaji, J. Ogata, A. Seiko, N. Okamoto, M. Uehara, K. Matsui, H. Yazawa, H. Sueta, Kazumasa Yamagishi, S. Akiba, H. Yamaguchi, T. Shimazu, S. Kono, Y. Shirai, I. Asano, Y. Tanaba, N. Tsuchiya, H. Sugimura, Y. Hatayama, S. Tsugane, I. Hashimoto, N. Nagai, Y. Matsumura, K. Miyakawa, A. Okayama, Akiko Nanri, A. Terao, T. Minamizono, K. Suzuki, M. Urata, S. Natsukawa, T. Fukuyama, Tetsuya Mizoue, Shoichiro Tsugane, J. Ishihara, Nobuyuki Hamajima, Y. Honda, M. Katagiri, Y. Yoshida, M. Inoue, H. Sato, Ribeka Takachi, K. Kobayashi, R. Saito, Sangah Shin, M. Irei, R. Takachi, Y. Ishikawa, Y. Kawaguchi, Tomotaka Sobue, Eiko Saito, S. Nagasawa, Mitsuhiko Noda, Taichi Shimazu, T. Nakasone, M. Kabuto, Nobufumi Yasuda, Isao Saito, K. Okada, Yukiaki Miyagawa, M. Akabane, F. Kobayashi, T. Hanaoka, S. Sasaki, M. Suzuki, A. Ioka, F. Ide, F. Shoji, Y. Kobayashi, S. Sasazuki, Hiroshi Sakiyama, M. Yamakawa, K. Motegi, H. Shimizu, S. Yamato, Shizuka Sasazuki, A. Murata, Junko Ishihara, F. Ito, M. Tsukada, Toshifumi Mannami, S. Baba, F. Horii, Motoki Iwasaki, H. Uchino, W. Ajiki, Takashi Kadowaki, T. Takashima, Y. Furusugi, N. Onga, Masamitsu Konishi, S. Watanabe, A. Koizumi, T. Ikuta, M. Takano, H. Doi, S. Maruyama, Yasuhiro Takashima, Y. Sano, H. Sanada, M. Yamaguchi, E. Maruyama, M. Machida, R. Fujita, H. Takaesu, F. Saito, and Manami Inoue
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Adult ,Male ,Gerontology ,medicine.medical_specialty ,Colorectal cancer ,Critical Care and Intensive Care Medicine ,Diet Surveys ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Risk Factors ,Surveys and Questionnaires ,Epidemiology ,medicine ,Humans ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Aged ,Nutrition and Dietetics ,business.industry ,Public health ,Confounding ,Cancer ,Middle Aged ,Dietary pattern ,medicine.disease ,Diet ,030220 oncology & carcinogenesis ,Cohort ,Female ,Colorectal Neoplasms ,business ,Demography - Abstract
A finding between dietary pattern and cancer may provide visions beyond the assessment of individual foods or nutrients. We examined the influence of dietary pattern with colorectal cancer (CRC) among a Japanese population.A total of 93,062 subjects (43,591 men, 49,471 women) who participated in the Japan Public Health Center-based Prospective Study were followed from 1995-1998 to the end of 2012, during which 2482 cases of CRC (1514 men, 968 women) were newly identified. Dietary data was obtained from a validated food-frequency questionnaire between 1995 and 1998.Three dietary pattern was derived from principal components factor: prudent, westernized, and traditional pattern. After controlled for potential confounders, the prudent pattern showed a decreased association of CRC risk in men (HR for highest quintile vs lowest: 0.85; 95% CI: 0.72-1.00; P trend0.05), slightly more strongly with distal colon cancer (P trend0.05); but an increased risk of rectal cancer in women (P trend0.05). The westernized pattern showed a significant positive linear trend for colon (P trend0.05) and distal cancer (P trend0.05) in women. There was no apparent association of traditional Japanese dietary pattern on the overall or any specific sites risk of CRC.A prudent dietary pattern showed an inverse association with CRC risk in men, and a westernized pattern was related with a higher risk of colon and distal cancer in women.
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- 2018
7. J-PARC-HI Collaboration
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J.K. Ahn, Y. Akamatsu, M. Asakawa, S. Ashikaga, O. Busch, E. Chishiro, M. Chiu, T. Chujo, P. Cirkovic, T. Csörgő, G. David, D. Devetak, M. Djordjevic, S. Esumi, H. Fujii, K. Fukushima, P. Garg, T. Gunji, T. Hachiya, H. Hamagaki, H. Harada, M. Harada, S. Hasegawa, Y. Hashimoto, T. Hatsuda, N. Hayashi, K. Hirano, T. Hirano, B.S. Hong, H. Hotchi, S.H. Hwang, Y. Ichikawa, T. Ichisawa, K. Imai, M. Inaba, K. Ishii, K. Itakura, T. Ito, J. Kamiya, M. Kaneta, H. Kato, S. Kato, N. Kikuzawa, B.C. Kim, E.J. Kim, T. Kimura, M. Kinsho, R. Kitamura, M. Kitazawa, Y. Kondo, A. Kovalenko, H. Kuboki, Y. Kurimoto, Y. Liu, X. Luo, T. Maruyama, S. Meigo, Y. Miake, A. Miura, T. Miyao, J. Milosevic, D. Mishra, T. Morishita, K. Morita, Y. Morita, K. Moriya, K. Murase, R. Muto, L. Nadjdjerdj, S. Nagamiya, A. Nakamura, T. Nakamura, T. Nakanoya, Y. Nara, M. Naruki, K. Niki, K. Nishio, C. Nonaka, T. Nonaka, M. Ogino, H. Oguri, C. Ohmori, A. Ohnishi, M. Oka, A. Okabe, M. Okamura, K. Oyama, K. Ozawa, P.K. Saha, T.R. Saito, A. Sakaguchi, T. Sakaguchi, S. Sakai, H. Sako, K. Sato, S. Sato, Y. Sato, S. Sawada, T. Shibata, K. Shigaki, S. Shimansky, T. Shimokawa, M. Shimomura, K. Shindo, S. Shinozaki, M. Shirakata, Y. Shobuda, M. Stojanovic, K. Suganuma, H. Sugimura, Y. Sugiyama, H. Takahashi, T. Takayanagi, Y. Takeuchi, F. Tamura, H. Tamura, J. Tamura, K.H. Tanaka, Y. Tanaka, N. Tani, K. Tanida, M. Tomisawa, T. Toyama, Y. Watanabe, N. Xu, K. Yamamoto, M. Yamamoto, S. Yokkaichi, I.K. Yoo, M. Yoshii, and M. Yoshimoto
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Nuclear and High Energy Physics - Published
- 2021
8. Coping strategies and risk of cardiovascular disease incidence and mortality: the Japan Public Health Center-based prospective Study
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Thomas, Svensson, Manami, Inoue, Norie, Sawada, Kazumasa, Yamagishi, Hadrien, Charvat, Isao, Saito, Yoshihiro, Kokubo, Hiroyasu, Iso, Noriyuki, Kawamura, Kenji, Shibuya, Masaru, Mimura, Shoichiro, Tsugane, S, Tsugane, N, Sawada, M, Iwasaki, S, Sasazuki, T, Shimazu, T, Yamaji, T, Hanaoka, J, Ogata, S, Baba, T, Mannami, A, Okayama, Y, Kokubo, K, Miyakawa, F, Saito, A, Koizumi, Y, Sano, I, Hashimoto, T, Ikuta, Y, Tanaba, H, Sato, Y, Roppongi, T, Takashima, Y, Miyajima, N, Suzuki, S, Nagasawa, Y, Furusugi, N, Nagai, Y, Ito, S, Komatsu, T, Minamizono, H, Sanada, Y, Hatayama, F, Kobayashi, H, Uchino, Y, Shirai, T, Kondo, R, Sasaki, Y, Watanabe, Y, Miyagawa, Y, Kobayashi, M, Machida, K, Kobayashi, M, Tsukada, Y, Kishimoto, E, Takara, T, Fukuyama, M, Kinjo, M, Irei, H, Sakiyama, K, Imoto, H, Yazawa, T, Seo, A, Seiko, F, Ito, F, Shoji, R, Saito, A, Murata, K, Minato, K, Motegi, T, Fujieda, S, Yamato, K, Matsui, T, Abe, M, Katagiri, M, Suzuki, M, Doi, A, Terao, Y, Ishikawa, T, Tagami, H, Sueta, H, Doi, M, Urata, N, Okamoto, F, Ide, H, Goto, N, Onga, H, Takaesu, M, Uehara, T, Nakasone, M, Yamakawa, F, Horii, I, Asano, H, Yamaguchi, K, Aoki, S, Maruyama, M, Ichii, M, Takano, Y, Tsubono, K, Suzuki, Y, Honda, K, Yamagishi, S, Sakurai, N, Tsuchiya, M, Kabuto, M, Yamaguchi, Y, Matsumura, S, Sasaki, S, Watanabe, M, Akabane, T, Kadowaki, M, Inoue, M, Noda, T, Mizoue, Y, Kawaguchi, Y, Takashima, Y, Yoshida, K, Nakamura, R, Takachi, J, Ishihara, S, Matsushima, S, Natsukawa, H, Shimizu, H, Sugimura, S, Tominaga, N, Hamajima, H, Iso, T, Sobue, M, Iida, W, Ajiki, A, Ioka, S, Sato, E, Maruyama, M, Konishi, K, Okada, I, Saito, N, Yasuda, S, Kono, and S, Akiba
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Male ,Coping (psychology) ,medicine.medical_specialty ,Myocardial Infarction ,Kaplan-Meier Estimate ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Risk Factors ,Internal medicine ,Adaptation, Psychological ,medicine ,Humans ,Prospective Studies ,030212 general & internal medicine ,Prospective cohort study ,Stroke ,Aged ,Proportional hazards model ,business.industry ,Incidence ,Incidence (epidemiology) ,Hazard ratio ,Avoidance coping ,Middle Aged ,medicine.disease ,Cardiovascular Diseases ,Cohort ,Physical therapy ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Aims Coping strategies may be significantly associated with health outcomes. This is the first study to investigate the association between baseline coping strategies and cardiovascular disease (CVD) incidence and mortality in a general population cohort. Methods and results The Japan Public Health Center-based prospective Study asked questions on coping in its third follow-up survey (2000–04). Analyses on CVD incidence and mortality included 57 017 subjects aged 50–79 without a history of CVD and who provided complete answers on approach- and avoidance-oriented coping behaviours and strategies. Cox regression models, adjusted for confounders, were used to determine hazard ratios (HRs) according to coping style. Mean follow-up time was 7.9 years for incidence and 8.0 years for mortality. The premorbid use of an approach-oriented coping strategy was inversely associated with incidence of stroke (HR = 0.85; 95% CI, 0.73–1.00) and CVD mortality (HR = 0.74; 95% CI, 0.55–0.99). Stroke subtype analyses revealed an inverse association between the approach-oriented coping strategy and incidence of ischaemic stroke (HR = 0.79; 95% CI, 0.64–0.98) and a positive association between the combined coping strategy and incidence of intra-parenchymal haemorrhage (HR = 2.03; 95% CI, 1.01–4.10). Utilizing an avoidance coping strategy was associated with increased mortality from ischaemic heart disease (IHD) only in hypertensive individuals (HR = 3.46; 95% CI, 1.07–11.18). The coping behaviours fantasizing and positive reappraisal were associated with increased risk of CVD incidence (HR = 1.24; 95% CI, 1.03–1.50) and reduced risk of IHD mortality (HR = 0.63; 95% CI, 0.40–0.99), respectively. Conclusion An approach-oriented coping strategy, i.e. proactively dealing with sources of stress, may be associated with significantly reduced stroke incidence and CVD mortality in a Japanese population-based cohort.
- Published
- 2016
9. First Determination of the Level Structure of an sd-Shell Hypernucleus, _{Λ}^{19}F
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S B, Yang, J K, Ahn, Y, Akazawa, K, Aoki, N, Chiga, H, Ekawa, P, Evtoukhovitch, A, Feliciello, M, Fujita, S, Hasegawa, S, Hayakawa, T, Hayakawa, R, Honda, K, Hosomi, S H, Hwang, N, Ichige, Y, Ichikawa, M, Ikeda, K, Imai, S, Ishimoto, S, Kanatsuki, S H, Kim, S, Kinbara, K, Kobayashi, T, Koike, J Y, Lee, K, Miwa, T J, Moon, T, Nagae, Y, Nakada, M, Nakagawa, Y, Ogura, A, Sakaguchi, H, Sako, Y, Sasaki, S, Sato, K, Shirotori, H, Sugimura, S, Suto, S, Suzuki, T, Takahashi, H, Tamura, K, Tanida, Y, Togawa, Z, Tsamalaidze, M, Ukai, T F, Wang, and T O, Yamamoto
- Abstract
We report on the first observation of γ rays emitted from an sd-shell hypernucleus, _{Λ}^{19}F. The energy spacing between the ground state doublet, 1/2^{+} and 3/2^{+} states, of _{Λ}^{19}F is determined to be 315.5±0.4(stat)_{-0.5}^{+0.6}(syst) keV by measuring the γ-ray energy of the M1(3/2^{+}→1/2^{+}) transition. In addition, three γ-ray peaks are observed and assigned as E2(5/2^{+}→1/2^{+}), E1(1/2^{-}→1/2^{+}), and E1(1/2^{-}→3/2^{+}) transitions. The excitation energies of the 5/2^{+} and 1/2^{-} states are determined to be 895.2±0.3(stat)±0.5(syst) and 1265.6±1.2(stat)_{-0.5}^{+0.7}(syst) keV, respectively. It is found that the ground state doublet spacing is well described by theoretical models based on existing s- and p-shell hypernuclear data.
- Published
- 2018
10. Plasma levels of n-3 fatty acids and risk of coronary heart disease among Japanese: The Japan Public Health Center-based (JPHC) study
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Kei Hamazaki, Hiroyasu Iso, Ehab S. Eshak, Satoyo Ikehara, Ai Ikeda, Motoki Iwasaki, Tomohito Hamazaki, Shoichiro Tsugane, S. Tsugane, N. Sawada, M. Iwasaki, S. Sasazuki, T. Yamaji, T. Shimazu, A. Goto, A. Hidaka, T. Hanaoka, J. Ogata, S. Baba, T. Mannami, A. Okayama, Y. Kokubo, K. Miyakawa, F. Saito, A. Koizumi, Y. Sano, I. Hashimoto, T. Ikuta, Y. Tanaba, H. Sato, Y. Roppongi, T. Takashima, H. Suzuki, Y. Miyajima, N. Suzuki, S. Nagasawa, Y. Furusugi, N. Nagai, Y. Ito, S. Komatsu, T. Minamizono, H. Sanada, Y. Hatayama, F. Kobayashi, H. Uchino, Y. Shirai, T. Kondo, R. Sasaki, Y. Watanabe, Y. Miyagawa, Y. Kobayashi, M. Machida, K. Kobayashi, M. Tsukada, Y. Kishimoto, E. Takara, T. Fukuyama, M. Kinjo, M. Irei, H. Sakiyama, K. Imoto, H. Yazawa, T. Seo, A. Seiko, F. Ito, F. Shoji, R. Saito, A. Murata, K. Minato, K. Motegi, T. Fujieda, S. Yamato, M. Doi, K. Matsui, T. Abe, M. Katagiri, M. Suzuki, A. Terao, Y. Ishikawa, T. Tagami, H. Sueta, H. Doi, M. Urata, N. Okamoto, F. Ide, H. Goto, R. Fujita, Y. Sou, N. Onga, H. Takaesu, M. Uehara, T. Nakasone, M. Yamakawa, F. Horii, I. Asano, H. Yamaguchi, K. Aoki, S. Maruyama, M. Ichii, M. Takano, Y. Tsubono, K. Suzuki, Y. Honda, K. Yamagishi, S. Sakurai, N. Tsuchiya, M. Kabuto, M. Yamaguchi, Y. Matsumura, S. Sasaki, S. Watanabe, M. Akabane, T. Kadowaki, M. Inoue, M. Noda, T. Mizoue, Y. Kawaguchi, Y. Takashima, Y. Yoshida, K. Nakamura, R. Takachi, J. Ishihara, S. Matsushima, S. Natsukawa, H. Shimizu, H. Sugimura, S. Tominaga, N. Hamajima, H. Iso, T. Sobue, M. Iida, W. Ajiki, A. Ioka, S. Sato, E. Maruyama, M. Konishi, K. Okada, I. Saito, N. Yasuda, S. Kono, S. Akiba, T. Isobe, and Y. Sato
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Adult ,Male ,medicine.medical_specialty ,Coronary Disease ,030204 cardiovascular system & hematology ,Sudden cardiac death ,03 medical and health sciences ,0302 clinical medicine ,Japan ,Risk Factors ,Internal medicine ,Surveys and Questionnaires ,Fatty Acids, Omega-3 ,Fish Products ,medicine ,Odds Ratio ,Humans ,030212 general & internal medicine ,Myocardial infarction ,Aged ,chemistry.chemical_classification ,Meal ,business.industry ,Odds ratio ,Feeding Behavior ,Middle Aged ,medicine.disease ,Confidence interval ,Death, Sudden, Cardiac ,chemistry ,Quartile ,Case-Control Studies ,Cohort ,Multivariate Analysis ,Female ,Public Health ,Cardiology and Cardiovascular Medicine ,business ,Polyunsaturated fatty acid ,Follow-Up Studies - Abstract
Background and aims Higher intake of fish or n-3 polyunsaturated fatty acids (PUFAs) has been associated with reduced risk of coronary heart disease (CHD). However, it is unclear whether increased blood levels of n-3 PUFAs are associated with reduced risk of CHD in the Japanese population. Methods The relationship between circulating levels of n-3 PUFAs (eicosapentaenoic acid + docosapentaenoic acid + docosahexaenoic acid) and risk of CHD was examined in a nested case-control study among participants in the Japan Public Health Center (JPHC)-based Study Cohort. Plasma n-3 PUFA phospholipid levels were measured at baseline by gas chromatography in 209 cases with CHD and 418 controls matched for sex, age, date of blood draw, time elapsed since last meal before blood collection, and study location. The CHD cases (n = 209) comprised 168 cases of myocardial infarction and 41 of sudden cardiac death, otherwise classified as 157 non-fatal and 52 fatal coronary events, respectively. Mean duration of follow-up was 13.5 years. Results Multivariate conditional logistic analysis showed no significant association between n-3 PUFAs and risk of total CHD. The odds ratio (OR) for the highest versus lowest quartiles of plasma n-3 PUFAs was 0.79 (95% confidence interval [95% CI]: 0.41–1.51, p for trend = 0.51). Subtype analysis of CHD revealed that the multivariate ORs for the highest versus lowest quartiles for n-3 PUFAs were 0.91 (95% CI: 0.43–1.89, p for trend = 0.90) for myocardial infarction, 0.08 (95% CI: 0.01–0.88, p for trend = 0.04) for sudden cardiac death, 0.89 (95% CI: 0.42–1.89, p for trend = 0.97) for nonfatal coronary events, and 0.12 (95% CI: 0.02–0.75, p for trend = 0.03) for fatal coronary events. Conclusions Plasma n-3 PUFA levels were not associated with risk of total CHD but were inversely associated with risks of sudden cardiac death and fatal coronary events among middle-aged Japanese individuals.
- Published
- 2017
11. MON-PO607: Relationship Between Nutritional Assessment and Deterioration of Activities of Daily Living in Patients with Lung Cancer
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H. Sugimura, Y. Ueta, and K. Miyakoshi
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medicine.medical_specialty ,Nutrition and Dietetics ,Activities of daily living ,business.industry ,Internal medicine ,medicine ,In patient ,Critical Care and Intensive Care Medicine ,Lung cancer ,medicine.disease ,business - Published
- 2019
12. [JSNP Excellent Presentation Award for CINP2014]
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D, Nishizawa, S, Kasai, J, Hasegawa, N, Sato, F, Tanioka, M, Nagashima, H, Ujike, R, Hashimoto, M, Tanaka, H, Sugimura, and K, Ikeda
- Subjects
Polymorphism, Genetic ,Genome, Human ,Awards and Prizes ,Humans ,Tobacco Use Disorder ,Genome-Wide Association Study - Published
- 2015
13. Association of green tea consumption with mortality due to all causes and major causes of death in a Japanese population: the Japan Public Health Center-based Prospective Study (JPHC Study)
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Eiko Saito, Manami Inoue, Norie Sawada, Taichi Shimazu, Taiki Yamaji, Motoki Iwasaki, Shizuka Sasazuki, Mitsuhiko Noda, Hiroyasu Iso, Shoichiro Tsugane, S. Tsugane, N. Sawada, M. Iwasaki, S. Sasazuki, T. Yamaji, T. Shimazu, T. Hanaoka, J. Ogata, S. Baba, T. Mannami, A. Okayama, Y. Kokubo, K. Miyakawa, F. Saito, A. Koizumi, Y. Sano, I. Hashimoto, T. Ikuta, Y. Tanaba, H. Sato, Y. Roppongi, T. Takashima, Y. Miyajima, N. Suzuki, S. Nagasawa, Y. Furusugi, N. Nagai, Y. Ito, S. Komatsu, T. Minamizono, H. Sanada, Y. Hatayama, F. Kobayashi, H. Uchino, Y. Shirai, T. Kondo, R. Sasaki, Y. Watanabe, Y. Miyagawa, Y. Kobayashi, M. Machida, K. Kobayashi, M. Tsukada, Y. Kishimoto, E. Takara, T. Fukuyama, M. Kinjo, M. Irei, H. Sakiyama, K. Imoto, H. Yazawa, T. Seo, A. Seiko, F. Ito, F. Shoji, R. Saito, A. Murata, K. Minato, K. Motegi, T. Fujieda, S. Yamato, K. Matsui, T. Abe, M. Katagiri, M. Suzuki, M. Doi, A. Terao, Y. Ishikawa, T. Tagami, H. Sueta, H. Doi, M. Urata, N. Okamoto, F. Ide, H. Goto, N. Onga, H. Takaesu, M. Uehara, T. Nakasone, M. Yamakawa, F. Horii, I. Asano, H. Yamaguchi, K. Aoki, S. Maruyama, M. Ichii, M. Takano, Y. Tsubono, K. Suzuki, Y. Honda, K. Yamagishi, S. Sakurai, N. Tsuchiya, M. Kabuto, M. Yamaguchi, Y. Matsumura, S. Sasaki, S. Watanabe, M. Akabane, T. Kadowaki, M. Inoue, M. Noda, T. Mizoue, Y. Kawaguchi, Y. Takashima, Y. Yoshida, K. Nakamura, R. Takachi, J. Ishihara, S. Matsushima, S. Natsukawa, H. Shimizu, H. Sugimura, S. Tominaga, N. Hamajima, H. Iso, T. Sobue, M. Iida, W. Ajiki, A. Ioka, S. Sato, E. Maruyama, M. Konishi, K. Okada, I. Saito, N. Yasuda, S. Kono, and S. Akiba
- Subjects
Gerontology ,Adult ,Male ,medicine.medical_specialty ,Epidemiology ,Population ,Respiratory Tract Diseases ,Poison control ,Sex Factors ,Asian People ,Japan ,Risk Factors ,Environmental health ,Cause of Death ,Injury prevention ,medicine ,Humans ,Prospective Studies ,Mortality ,education ,Prospective cohort study ,Aged ,Proportional Hazards Models ,education.field_of_study ,Tea ,Proportional hazards model ,business.industry ,Public health ,Incidence ,Hazard ratio ,Middle Aged ,Cardiovascular Diseases ,Female ,business ,Cohort study - Abstract
We examined the association between green tea consumption and mortality due to all causes, cancer, heart disease, cerebrovascular disease, respiratory disease, injuries, and other causes of death in a large-scale population-based cohort study in Japan.We studied 90,914 Japanese (aged between 40 and 69 years) recruited between 1990 and 1994. After 18.7 years of follow-up, 12,874 deaths were reported. The association between green tea consumption and risk of all causes and major causes of mortality was assessed using the Cox proportional hazards regression model with adjustment for potential confounders.Hazard ratios for all-cause mortality among men who consumed green tea compared with those who drank less than 1 cup/day were 0.96 (0.89-1.03) for 1-2 cups/day, 0.88 (0.82-0.95) for 3-4 cups/day, and 0.87 (0.81-0.94) for more than 5 cups/day (P for trend.001). Corresponding hazard ratios for women were 0.90 (0.81-1.00), 0.87 (0.79-0.96), and 0.83 (0.75-0.91; P for trend.001). Green tea was inversely associated with mortality from heart disease in both men and women and mortality from cerebrovascular disease and respiratory disease in men. No association was found between green tea and total cancer mortality.This prospective study suggests that the consumption of green tea may reduce the risk of all-cause mortality and the three leading causes of death in Japan.
- Published
- 2014
14. Molecular-Resolution Imaging of Ionic Liquid/Alkali Halide Interfaces with Varied Surface Charge Densities via Atomic Force Microscopy.
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Bao Y, Nishiwaki Y, Kawano T, Utsunomiya T, Sugimura H, and Ichii T
- Abstract
When in contact with charged solid surfaces, ionic liquids (ILs) are known to form solvation structures consisting of alternating cation and anion layers. This phenomenon is considered to originate from the adsorption layer of counterions overcompensating the surface charge, so-called overscreening. However, the response of these layers to surfaces with near-zero or extremely high surface charge density (σ) remains inadequately understood. Here, we probe the solvation structure of ILs on alkali halide surfaces with varied surface orientations: nearly zero-charged RbI(100) and highly charged RbI(111), by employing frequency modulation atomic force microscopy with atomic resolution. Two commonly used ILs are examined in this study: 1-methyl-1-propylpyrrolidinium bis(trifluoromethylsulfonyl)imide ([C
3 mpyr][NTf2 ]) and 1-ethyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C2 mim][NTf2 ]). On RbI(100) surfaces with near zero σ, we observe alternating cation and anion layers, diverging from the previously proposed monolayer model for IL/alkali halide(100) interfaces. These results support the argument that overscreening occurs under low σ, even approaching zero, and reconcile conflicting experimental conclusions about low σ systems. On RbI(111) surfaces with high σ, we identify solvation structures consisting of two consecutive counterion layers. This structure aligns with the theoretically predicted crowding; a phenomenon rarely observed in commonly used ILs due to typically unreachable σ in electrochemical IL/electrode systems. Our findings indicate that alkali halide(111) surfaces are potentially valuable for exploring the crowding phenomenon in ILs, addressing the current scarcity of experimental observations.- Published
- 2024
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15. Stereoselective Synthesis of Spirolactone Analogues of Pyrrolomorpholine Alkaloids.
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Uchiyama A, Ohta H, Ogawa Y, Sasaki I, and Sugimura H
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The synthesis of a spirolactone analogue of xylapyrraside B
1 , a potent antioxidant agent, is described. The key step is the stereoselective formation of the spirolactone skeleton via the formal [3+2] annulation of the isopropylidene-protected glyceraldehyde and δ-methylene lactone, mediated by trifluoroborane etherate. This study addresses the stereoselective synthesis of pyrrolomorpholine spiroketal alkaloids, enabling the production of these bioactive compounds and their analogues.- Published
- 2024
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16. Effects of salt intake reduction by urinary sodium to potassium ratio self-monitoring method.
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Shimoyama M, Kawamoto S, Nakatani Y, Banba N, Nagashima Y, Tomoe T, Sugiyama T, Ueno A, Kitahara K, Kawabe A, Otani N, Sugimura H, and Yasu T
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- Humans, Female, Male, Middle Aged, Aged, Sodium Chloride, Dietary administration & dosage, Sodium Chloride, Dietary urine, Diet, Sodium-Restricted, Adult, Blood Pressure physiology, Sodium urine, Potassium urine, Hypertension urine
- Abstract
Effective and feasible educational methods are needed to control salt intake. We performed a single-center, non-randomized controlled study to investigate the effectiveness and feasibility of self-monitoring using a urinary sodium/potassium (Na/K) ratio-measuring device in patients with difficulty in reducing salt intake. This study included 160 patients with hypertension, chronic kidney disease, or heart disease who were followed up in the outpatient clinic of the Dokkyo Medical University Nikko Medical Center. Urinary Na/K ratio measuring Na/K ratio meter were loaned for 2-6 weeks to the treatment (T) group (n = 80) and not to the patients in the control (C) group (n = 80). In the T group, patients were instructed to measure the urinary Na/K ratio at least three times a day and maintain a Na/K ratio below 2.0. Salt reduction education and home blood pressure measurement guidance continued in both groups. The mean device loan period in the T group was 25.1 days, the mean number of measurements was 3.0 times/day, and the proportion of patients achieving three measurements per day was 48.8% (39/80). Self-monitoring using the urinary Na/K ratio meter successfully reduced salt intake by -1.9 g/day at the second visit (p < 0.001) in the T group. In contrast, no change was observed over time in the C group. Self-monitoring using the urinary Na/K ratio meter successfully reduced salt intake in patients with difficulty reducing salt intake., (© 2024. The Author(s), under exclusive licence to The Japanese Society of Hypertension.)
- Published
- 2024
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17. Bioinformatic Identification of TP53 Gene Mutation Hotspots in Colorectal Cancer.
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Kovács Z, Sugimura H, György TA, Osvath E, Manirakiza F, and Gurzu S
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- Humans, Male, Female, Middle Aged, Aged, Genetic Predisposition to Disease, Colorectal Neoplasms genetics, Colorectal Neoplasms pathology, Computational Biology methods, Mutation, Tumor Suppressor Protein p53 genetics, Exons genetics
- Abstract
Mutations and inactivation of the TP53 gene are frequently observed in various types of malignancies. Precise knowledge of the genetic structure and detection of mutation hotspots are crucial, as these indicate a high probability of developing cancer. The aim of our study was to perform the bioinformatic detection of mutation hotspots in the TP53 gene in patients diagnosed with malignant colon neoplasms using self-developed software (version 1). We compared TP53 gene sequences from 50 healthy individuals with those from 50 patients diagnosed with colorectal carcinoma. Of the 50 samples from cancer patients, the most frequent mutations were observed in exons 5 and 8 (12 mutations per exon) and gene sequences of 12 samples, which differed from those of the 50 samples from healthy individuals. Based on our results, the distribution of mutations in the TP53 gene structure was not even across different exons. By comparing the gene sequences of healthy individuals with those of colon cancer samples, we conclude that structural changes occurring in similar gene regions are not associated with increases in susceptibility to malignancies in every case, namely, that the pathological mechanism is multifactorial.
- Published
- 2024
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18. Successful Transvenous Implantation of a Permanent Pacemaker in a Patient with Situs Inversus with Dextrocardia Supported by Preceding Three-dimensional Computed Tomography.
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Nomura A, Otani N, Kokubun A, Mizuguchi S, Kawamoto S, Tomoe T, Kitahara K, Sugiyama T, Horie Y, Sugimura H, and Yasu T
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- Humans, Female, Aged, Sick Sinus Syndrome therapy, Sick Sinus Syndrome diagnostic imaging, Sick Sinus Syndrome complications, Prosthesis Implantation methods, Prosthesis Implantation instrumentation, Treatment Outcome, Dextrocardia diagnostic imaging, Dextrocardia complications, Situs Inversus diagnostic imaging, Situs Inversus complications, Pacemaker, Artificial, Imaging, Three-Dimensional, Tomography, X-Ray Computed
- Abstract
We herein report a 76-year-old woman with situs inversus and dextrocardia who underwent pacemaker implantation for sick sinus syndrome. Situs inversus with dextrocardia, which is frequently associated with cardiovascular malformation, is a rare congenital malformation wherein the thoracic and abdominal viscera are inverted compared with their normal positions. This renders the implantation of cardiac devices an arduous task. We therefore decided to gather preoperative anatomical information on patients with situs inversus and dextrocardia. We used three-dimensional computed tomography to collect preoperative information in order to facilitate the safe implantation of cardiac devices.
- Published
- 2024
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19. Correction: The spectrum of TP53 mutations in Rwandan patients with gastric cancer.
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Nzitakera A, Surwumwe JB, Ndoricyimpaye EL, Uwamungu S, Uwamariya D, Manirakiza F, Ndayisaba MC, Ntakirutimana G, Seminega B, Dusabejambo V, Rutaganda E, Kamali P, Ngabonziza F, Ishikawa R, Rugwizangoga B, Iwashita Y, Yamada H, Yoshimura K, Sugimura H, and Shinmura K
- Published
- 2024
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20. The spectrum of TP53 mutations in Rwandan patients with gastric cancer.
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Nzitakera A, Surwumwe JB, Ndoricyimpaye EL, Uwamungu S, Uwamariya D, Manirakiza F, Ndayisaba MC, Ntakirutimana G, Seminega B, Dusabejambo V, Rutaganda E, Kamali P, Ngabonziza F, Ishikawa R, Rugwizangoga B, Iwashita Y, Yamada H, Yoshimura K, Sugimura H, and Shinmura K
- Abstract
Background: Gastric cancer is the sixth most frequently diagnosed cancer and third in causing cancer-related death globally. The most frequently mutated gene in human cancers is TP53, which plays a pivotal role in cancer initiation and progression. In Africa, particularly in Rwanda, data on TP53 mutations are lacking. Therefore, this study intended to obtain TP53 mutation status in Rwandan patients with gastric cancer., Results: Formalin-fixed paraffin-embedded tissue blocks of 95 Rwandan patients with histopathologically proven gastric carcinoma were obtained from the University Teaching Hospital of Kigali. After DNA extraction, all coding regions of the TP53 gene and the exon-intron boundary region of TP53 were sequenced using the Sanger sequencing. Mutated TP53 were observed in 24 (25.3%) of the 95 cases, and a total of 29 mutations were identified. These TP53 mutations were distributed between exon 4 and 8 and most of them were missense mutations (19/29; 65.5%). Immunohistochemical analysis for TP53 revealed that most of the TP53 missense mutations were associated with TP53 protein accumulation. Among the 29 mutations, one was novel (c.459_477delCGGCACCCGCGTCCGCGCC). This 19-bp deletion mutation in exon 5 caused the production of truncated TP53 protein (p.G154Wfs*10). Regarding the spectrum of TP53 mutations, G:C > A:T at CpG sites was the most prevalent (10/29; 34.5%) and G:C > T:A was the second most prevalent (7/29; 24.1%). Interestingly, when the mutation spectrum of TP53 was compared to three previous TP53 mutational studies on non-Rwandan patients with gastric cancer, G:C > T:A mutations were significantly more frequent in this study than in our previous study (p = 0.013), the TCGA database (p = 0.017), and a previous study on patients from Hong Kong (p = 0.006). Even after correcting for false discovery, statistical significance was observed., Conclusions: Our results suggested that TP53 G:C > T:A transversion mutation in Rwandan patients with gastric cancer is more frequent than in non-Rwandan patients with gastric cancer, indicating at an alternative etiological and carcinogenic progression of gastric cancer in Rwanda., (© 2024. The Author(s).)
- Published
- 2024
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21. Left Main Bronchus Obstruction in a Patient with Small-cell Lung Cancer Successfully Treated with Venovenous Extracorporeal Membrane Oxygenation.
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Nagai T, Yoshida K, Otsuki A, So Y, Karumai T, Sugimura H, Tachibana Y, Fukuoka J, Ito H, and Nakashima K
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- Humans, Bronchi, Extracorporeal Membrane Oxygenation, Lung Neoplasms complications, Lung Neoplasms therapy, Respiratory Insufficiency etiology, Respiratory Insufficiency therapy, Airway Obstruction therapy, Airway Obstruction complications, Respiratory Distress Syndrome, Small Cell Lung Carcinoma complications, Small Cell Lung Carcinoma therapy
- Abstract
Lung cancer can cause fatal central airway obstruction. Rapid airway clearance is necessary in some cases, but ventilator management may be insufficient to maintain oxygenation levels. Venovenous extracorporeal membrane oxygenation (VV-ECMO) may be an effective rescue therapy for respiratory failure, but its efficacy in treating tumor-related airway obstruction is unknown. We herein report a case of central airway obstruction and severe acute respiratory failure due to small-cell lung cancer successfully treated with VV-ECMO, bronchoscopic airway intervention, and chemotherapy. VV-ECMO can be an effective option for the treatment of central airway obstruction with acute respiratory failure due to lung cancer.
- Published
- 2024
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22. Relationship Between Increased Oxygen Uptake and Lactate Production With Progressive Incremental Electrode Skeletal Muscle Stimulation: A Pilot Study.
- Author
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Tamura Y, Ochiai K, Takahashi M, Takahashi H, Tomoe T, Sugiyama T, Otani N, Sugimura H, Toyoda S, and Yasu T
- Abstract
Background Belt electrode skeletal muscle stimulation (B-SES) is an alternative exercise therapy for those with difficulty performing voluntary exercise. However, it is unknown whether oxygen uptake (VO
2 ) in B-SES is comparable to cardiopulmonary exercise test (CPX) as assessed by voluntary exercise. This study aimed to evaluate oxygen uptake (VO2 ) and lactate (LA) production in incremental B-SES compared to ergometer CPX and to determine the relationship with ergometer CPX. Methods This study included 10 healthy young Japanese participants. Using a crossover design, all participants underwent incremental B-SES CPX and ergometer CPX using a 20 W ramp. Serum lactic acid concentration (LA) was measured serially before, during, and after B-SES. The tolerability of B-SES was adjusted with the change in LA level (⊿LA). Results Peak VO2 during B-SES (14.1±3.3 mL/kg/min) was significantly lower than ergometer peak VO2 (30.2±6.2 mL/kg/min, P<0.001). B-SES peak VO2 was similar to the anaerobic threshold (AT) VO2 on ergometer CPX (15.1±2.6 mL/kg/min). LA (Rest: 1.4±0.3, Peak: 2.8±0.8 mmol) and plasma noradrenalin (Rest: 0.2±0.1, Peak: 0.4±0.1 ng/mL) levels increased after B-SES. No significant correlation was observed between B-SES peak VO2 and ergometer CPX. However, after adjusting for B-SES, tolerability, it (peak VO2 of B-SES /⊿LA) correlated with peak VO2 (r=0.688, p=0.028) on the ergometer. Conclusion Peak VO2 of the passively progressive B-SES almost reached the AT value of the ergometer CPX without adverse events. Peak VO2 of B-SES adjusted with ⊿LA may be used to predict peak VO2 in ergometer CPX., Competing Interests: The authors have declared financial relationships, which are detailed in the next section., (Copyright © 2024, Tamura et al.)- Published
- 2024
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23. Risk Evaluation of Proton Pump Inhibitors for Panitumumab-Related Hypomagnesemia in Patients with Metastatic Colorectal Cancer.
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Katsura H, Suga Y, Kubo A, Sugimura H, Kumatani K, Haruki K, Yonezawa M, Narita A, Ishijima R, Ikesue H, Toi H, and Takata N
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- Humans, Panitumumab adverse effects, Proton Pump Inhibitors adverse effects, Retrospective Studies, Magnesium therapeutic use, Colonic Neoplasms drug therapy, Colorectal Neoplasms pathology
- Abstract
Hypomagnesemia commonly occurs as a side effect of panitumumab treatment. In severe cases, temporary discontinuation or dose reduction of panitumumab may be necessary. Proton pump inhibitors (PPIs) are reportedly potential risk factors for hypomagnesemia. We conducted a multicenter study to assess the impact of PPIs on the risk of grade 3-4 hypomagnesemia in patients with metastatic colorectal cancer (mCRC) receiving panitumumab. We adjusted for potential bias using a propensity score-matched analysis and retrospectively reviewed the medical records of patients. Hypomagnesemia severity was graded according to the Common Terminology Criteria for Adverse Events, version 5.0. A total of 165 patients were enrolled in this study. The incidence of grade 3-4 hypomagnesemia was significantly higher in the PPI group than in the non-PPI group, both before (20.0% [30/60] vs. 8.0% [8/105], p = 0.026) and after propensity score matching (16.2% [6/37] vs. 0% [0/37], p = 0.025). In the propensity score-matched cohort, the risk of grade 3-4 hypomagnesemia was significantly higher in the PPI group (odds ratio, 2.19; 95% confidence interval, 1.69-2.84; p = 0.025). These findings suggest that concomitant use of PPIs significantly increases the risk of grade 3-4 hypomagnesemia in patients with mCRC receiving panitumumab. Therefore, close monitoring of these patients is imperative.
- Published
- 2024
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24. DNA methylation status of SHATI/NAT8L promoter in the blood of cigarette smokers.
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Izuo N, Miyanishi H, Nishizawa D, Fujii T, Hasegawa J, Sato N, Tanioka F, Sugimura H, Ikeda K, and Nitta A
- Subjects
- Humans, DNA Methylation, Smokers, Tobacco Use Cessation Devices, Biomarkers, Acetyltransferases metabolism, Smoking Cessation, Tobacco Products
- Abstract
Aims: Cigarette smoking is a preventable risk factor for various diseases such as cancer, ischemic stroke, cardiac stroke, and chronic obstructive pulmonary disease. Smoking cessation is of great importance not only for individual smokers but also for social health. Regarding current cessation therapies, the effectiveness of nicotine replacement is limited, and the cost of varenicline medication is considerable. Thus, a method for screening smokers who are responsive to cessation therapy based on the therapeutic effectiveness is required. Peripheral biomarkers reflecting smoking dependence status are necessary to establish a method for achieving effective cessation therapy., Methods: Methylation status of smokers' blood DNA was evaluated focusing on SHATI/NAT8L, an addiction-related gene. Eight CpG sites in SHATI/NAT8L were quantified by pyrosequencing., Results: There was no difference in the methylation status of this gene between smokers (n = 129) and non-smokers (n = 129) at all CpG sites. No correlations between the methylation status of SHATI/NAT8L and indicators of smoking dependence were found., Conclusions: Although the present study found no significance in the DNA methylation of SHATI/NAT8L among smokers, the exploration of predictable peripheral biomarkers for the effectiveness of smoking cessation therapy is required., (© 2023 The Authors. Neuropsychopharmacology Reports published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Neuropsychopharmacology.)
- Published
- 2023
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25. High Expression of Fas-Associated Factor 1 Indicates a Poor Prognosis in Non-Small-Cell Lung Cancer.
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Hu, Yamada H, Yoshimura K, Ohta T, Tsuchiya K, Inoue Y, Funai K, Suda T, Iwashita Y, Watanabe T, Ogawa H, Kurono N, Shinmura K, and Sugimura H
- Subjects
- Humans, Adaptor Proteins, Signal Transducing genetics, Adaptor Proteins, Signal Transducing metabolism, Carrier Proteins genetics, Carrier Proteins metabolism, Fibrinogen, Prognosis, Apoptosis Regulatory Proteins genetics, Carcinoma, Non-Small-Cell Lung genetics, Lung Neoplasms genetics, Lung Neoplasms metabolism
- Abstract
Fas-associated factor 1 (FAF1) is a death-promoting protein identified as an interaction partner of the death receptor Fas. The downregulation and mutation of FAF1 have been reported in a variety of human tumors, but there have been few studies on lung cancer. Here, we investigated the prognostic significance of FAF1 expression in non-small-cell lung cancer (NSCLC), and whether aberrant FAF1 expression may be involved in the pathogenesis and prognosis of NSCLC. FAF1 expression was examined in NSCLC specimens as well as human lung cancer cell lines. In addition, changes in cell viability and apoptosis upon regulating FAF1 expression were investigated in lung cancer cell lines. As a result, high FAF1 expression was significantly associated with a poor prognosis in NSCLC. In lung cancer cell lines, FAF1 downregulation hindered cell viability and tended to promote early apoptosis. In conclusion, this is the first study of the clinical significance of FAF1 in NSCLC, showing that FAF1 overexpression is associated with a poor prognosis in NSCLC and that FAF1 acts as a dangerous factor rather than an apoptosis promoter in NSCLC.
- Published
- 2023
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26. Interaction between the substrate and probe in liquid metal Ga: experimental and theoretical analysis.
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Amano KI, Tozawa K, Tomita M, Takagi R, Iwayasu R, Nakano H, Murata M, Abe Y, Utsunomiya T, Sugimura H, and Ichii T
- Abstract
Interaction between two bodies in a liquid metal is an important topic for development of metallic products with high performance. We conducted atomic force microscopy measurements and achieved the interaction between the substrate and the probe in liquid Ga of an opaque and highly viscous liquid. The interaction cannot be accessed with the normal atomic force microscopy, electron microscopy, and beam reflectometry. We performed a theoretical calculation using statistical mechanics of simple liquids by mixing an experimentally derived quantum effect. From both experiment and theory, we found an unusual behaviour in the interaction between the solvophobic substances, which has never been reported in water and ionic liquids. Shapes of the interaction curves between several solvophobic and solvophilic pairs in liquid Ga are also studied., Competing Interests: There are no conflicts of interest to declare., (This journal is © The Royal Society of Chemistry.)
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- 2023
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27. Both MLH1 deficiency and BRAFV600E mutation are a unique characteristic of colorectal medullary carcinoma: An observational study.
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Kaneko M, Nakashima M, Sugiura K, Ishida N, Tamura S, Tani S, Yamade M, Hamaya Y, Osawa S, Tatsuta K, Kurachi K, Baba S, Iwashita Y, Arai T, Sugimura H, Maekawa M, Sugimoto K, and Iwaizumi M
- Subjects
- Humans, Mutation, Genetic Testing, MutL Protein Homolog 1 genetics, Carcinoma, Medullary, Adenocarcinoma, Colorectal Neoplasms genetics, Colorectal Neoplasms surgery
- Abstract
Although immunohistochemistry (IHC) for mismatch repair (MMR) proteins (MMR IHC) is used to identify DNA MMR status, universal screening of all patients with colorectal cancer (CRC) using a combination of both MMR IHC and genetic testing for the BRAFV600E mutation is limited in Japan. This study aimed to better understand the histopathological characteristics of CRCs, which exhibit both deficient mismatch repair (dMMR) and BRAFV600E mutation. MMR IHC of formalin-fixed paraffin-embedded tissues from tumor areas obtained from 651 patients with CRC who underwent surgical resection at Hamamatsu University Hospital (Hamamatsu, Japan) between August 2016 and March 2022 were used to evaluate MMR status, which was determined by staining for the expression of 4 MMR proteins (MLH1, MSH2, PMS2, and MSH6). All dMMR tumors were additionally evaluated for BRAFV600 mutation status via Sanger sequencing. Patient clinical characteristics (age, sex, tumor location, size, and tumor pathology) were then classified using their dMMR and BRAFV600 mutation statuses. Among the 651 patients with CRC, 58 carried tumors with dMMR, of which 52 were deficiency in MLH1 (dMLH1). Interestingly, all 16 medullary carcinomas that were analyzed showed characteristics corresponding to the presence of both dMLH1 and BRAFV600E mutation (P = .01). These results suggest that colorectal medullary carcinomas can be diagnosed based on their unique characteristics of harboring the BRAFV600E mutation and exhibiting dMLH1 expression., Competing Interests: The authors have no conflicts of interest to disclose., (Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.)
- Published
- 2023
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28. Evaluation of the widths of the mucosal strips in pathological examination of specimens of endoscopic submucosal dissection for early gastric cancer.
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Katsuragi SY, Otsuki Y, Unno S, Kimata M, Yoshizawa Y, Tomatsu M, Shinmura K, Suzuki K, and Sugimura H
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- Humans, Retrospective Studies, Gastric Mucosa surgery, Gastric Mucosa pathology, Gastroscopy methods, Gastrectomy methods, Treatment Outcome, Stomach Neoplasms surgery, Stomach Neoplasms pathology, Endoscopic Mucosal Resection methods
- Abstract
Background: Endoscopic submucosal dissection (ESD) is the standard treatment for early gastric cancer in Japan. Pathological evaluation of ESD specimens is considered essential to determine if additional gastrectomy is necessary. Usually, specimens resected by ESD are sliced into 2-3 mm wide sections, and each section is examined for depth of tumor and lymphovascular invasion. Nevertheless, in most cases of additional gastrectomy, lymph node metastasis is not present. Given that there are few-studies on how clinical-decisions based on the pathologic-evaluation-method, in particular the specimen cut-width, influence patient outcomes, we retrospectively evaluated whether reducing the number of cuts to one-half or one-third would result in underestimation of the real need for additional surgery. The effect of the actual cut-width on recommended treatment (referral to operation) and patient-outcomes was also assessed., Methods: Pathological records of 498 lesions from 439 patients were reviewed and re-evaluated. All pathological descriptions are based on the gastric cancer classification system of the Japanese Gastric Cancer Association, 15th edition., Results: In 5.8% and 8.5% of the total specimens, underdiagnosis of tumor-depth and lymphovascular invasion occurred when the number of sections was reduced to one-half and one-third, respectively. Significantly more submucosal invasions were found in the group in which the cut-with was between 3 and 4 mm than in the group in which the cut width was less than 3 mm., Conclusion: Evaluation of the appropriate cut-width is important and should be discussed from the standpoint of labor costs and lost opportunities to search for molecular markers in ESD materials., (© 2023. The Author(s) under exclusive licence to The International Gastric Cancer Association and The Japanese Gastric Cancer Association.)
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- 2023
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29. Lung adenocarcinoma and squamous cell carcinoma difficult for immunohistochemical diagnosis can be distinguished by lipid profile.
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Yamashita T, Takanashi Y, Uebayashi A, Oka M, Mizuno K, Kawase A, Oyama S, Kitamoto T, Kondo M, Omori S, Tao H, Takahashi Y, Sakamoto T, Kahyo T, Sugimura H, Setou M, Shiiya N, and Funai K
- Subjects
- Humans, Reproducibility of Results, Lipids, Biomarkers, Tumor analysis, Carcinoma, Non-Small-Cell Lung pathology, Lung Neoplasms diagnosis, Lung Neoplasms pathology, Adenocarcinoma of Lung, Carcinoma, Squamous Cell diagnosis, Carcinoma, Squamous Cell pathology, Adenocarcinoma diagnosis, Adenocarcinoma pathology
- Abstract
In patients with unresectable non-small cell lung cancer, histological diagnosis is frequently based on small biopsy specimens unsuitable for histological diagnosis when they are severely crushed and do not retain their morphology. Therefore, establishing a novel diagnostic method independent of tissue morphology or conventional immunohistochemistry (IHC) markers is required. We analyzed the lipid profiles of resected primary lung adenocarcinoma (ADC) and squamous cell carcinoma (SQCC) specimens using liquid chromatography-tandem mass spectrometry. The specimens of 26 ADC and 18 SQCC cases were evenly assigned to the discovery and validation cohorts. Non-target screening on the discovery cohort identified 96 and 13 lipid peaks abundant in ADC and SQCC, respectively. Among these 109 lipid peaks, six and six lipid peaks in ADC and SQCC showed reproducibility in target screening on the validation cohort. Finally, we selected three and four positive lipid markers for ADC and SQCC, demonstrating high discrimination abilities. In cases difficult to diagnose by IHC staining, [cardiolipin(18:2_18:2_18:2_18:2)-H]
- and [triglyceride(18:1_17:1_18:1) + NH4]+ showed the excellent diagnostic ability for ADC (sensitivity: 1.00, specificity: 0.89, accuracy: 0.93) and SQCC (sensitivity: 0.89, specificity: 0.83, accuracy: 0.87), respectively. These novel candidate lipid markers may contribute to a more accurate diagnosis and subsequent treatment strategy for unresectable NSCLC., (© 2023. The Author(s).)- Published
- 2023
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30. Association between intrapleural urokinase monotherapy and treatment failure in patients with pleural infection: a retrospective cohort study.
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Taniguchi J, Matsui H, Nagai T, Otsuki A, Ito H, Sugimura H, and Nakashima K
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- Humans, Urokinase-Type Plasminogen Activator therapeutic use, Hospital Mortality, Retrospective Studies, Treatment Failure, Empyema, Pleural therapy, Pleural Effusion drug therapy, Pleural Diseases drug therapy
- Abstract
Background: Pleural infection, an infection of the pleural space, is frequently treated with antibiotics and thoracic tube drainage. In case of insufficient drainage, an intrapleural fibrinolytic agent is considered before surgical intervention. However, the effectiveness of fibrinolytic monotherapy is still controversial. Therefore, we aimed to examine the association between urokinase monotherapy and treatment failure in patients with pleural infection., Methods: In this retrospective observational study, patients with pleural infection underwent chest tube insertion were divided into two groups including patients treated with or without intrapleural instillation of urokinase. The propensity score overlap weighting was used to balance the baseline characteristics between the groups. Treatment failure was defined by the composite primary outcome of in-hospital death and referral for surgery., Results: Among the 94 patients, 67 and 27 patients were in the urokinase and non-urokinase groups, respectively. Urokinase monotherapy improved the composite outcome between the groups (19.4% vs. 48.1%, p = 0.01). After adjusting using propensity score overlap weighting, urokinase monotherapy improved the composite outcome compared to the non-urokinase group (19.0% vs. 59.5%, p = 0.003)., Conclusions: Urokinase monotherapy can be an important nonsurgical treatment option for patients with pleural infection., Trial Registration: The participants were retrospectively registered., (© 2023. The Author(s).)
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- 2023
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31. TP53 mutations in Romanian patients with colorectal cancer.
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Manirakiza F, Yamada H, Iwashita Y, Ishino K, Ishikawa R, Kovacs Z, Osvath E, Nzitakera A, Gurzu S, and Sugimura H
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Background: Colorectal cancer (CRC) has been ranked as the second most deadly cancer and the third most diagnosed cancer cases for the year 2020. Specifically for Romania, the number of CRC-related deaths in 2019 was estimated at 6307 people, with a standardized mortality rate of 33.8 per 100,000 inhabitants. Although the tumor protein 53 (TP53) gene is intensively studied, there are few data on TP53 mutations in Romanian CRC. Furthermore, since genetic alterations may show geographical differences, our study aimed to analyze the clinical status and TP53 somatic variation in Romanian CRC patients., Subjects and Methods: DNA from 40 randomly selected cases of CRC was extracted from formalin-fixed paraffin-embedded tissues and sequenced using direct Sanger sequencing techniques, and variants were annotated according to the recommendations of the Human Genome Variation Society. Novel variants were analyzed using MutationTaster2021 to predict their effects., Results: The mean age was 63.6 years (range 33-85 years) with a male to female ratio of 2.3. More than 45% (18/40) had an advanced cancer stage (≥ stage III). Mutations were found in 21/40 cases (52.5%), with one case having two mutations, giving a total of twenty-two mutations in the TP53 coding DNA. These mutations include 3 (13.6%) insertion-deletion mutations, two of which are novel frameshift mutations: c.165delT (in exon 4) and c.928_935dup (in exon 9), both of which are predicted to lead to nonsense-mediated mRNA decay and are classified as deleterious. The remaining 19 (86.36%) were substitution mutations: 1 nonsense and 18 (81.8%) missense mutations, with G > A (n = 7/19; 36.8%) and C > T (n = 6/19; 31.5%) transitions being the most common. The G > T transversion was found in 21.05% (4/19) of the substitution mutations., Conclusion: We have described two novel frameshift mutations in TP53. The discovery of novel mutations following the efforts of The Cancer Genome Atlas and other large-scale cancer genome sequencing projects may be further evidence of the heterogeneous nature of mutations in cancer and may indicate that the identification of carcinogenic mutations is not yet saturated. Further sequencing is therefore needed, especially in less studied populations. Importantly, consideration of their geographical environment will shed light on population-specific carcinogenesis., (© 2023. The Author(s).)
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- 2023
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32. Clinicopathological Characteristics and Mutational Landscape of APC , HOXB13, and KRAS among Rwandan Patients with Colorectal Cancer.
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Manirakiza F, Rutaganda E, Yamada H, Iwashita Y, Rugwizangoga B, Seminega B, Dusabejambo V, Ntakirutimana G, Ruhangaza D, Uwineza A, Shinmura K, and Sugimura H
- Abstract
Cancer research in Rwanda is estimated to be less than 1% of the total African cancer research output with limited research on colorectal cancer (CRC). Rwandan patients with CRC are young, with more females being affected than males, and most patients present with advanced disease. Considering the paucity of oncological genetic studies in this population, we investigated the mutational status of CRC tissues, focusing on the Adenomatous polyposis coli (APC) , Kirsten rat sarcoma ( KRAS), and Homeobox B13 (HOXB13) genes. Our aim was to determine whether there were any differences between Rwandan patients and other populations. To do so, we performed Sanger sequencing of the DNA extracted from formalin-fixed paraffin-embedded adenocarcinoma samples from 54 patients (mean age: 60 years). Most tumors were located in the rectum (83.3%), and 92.6% of the tumors were low-grade. Most patients (70.4%) reported never smoking, and 61.1% of patients had consumed alcohol. We identified 27 variants of APC , including 3 novel mutations (c.4310_4319delAAACACCTCC, c.4463_4470delinsA, and c.4506_4507delT). All three novel mutations are classified as deleterious by MutationTaster2021. We found four synonymous variants (c.330C>A, c.366C>T, c.513T>C, and c.735G>A) of HOXB13 . For KRAS , we found six variants (Asp173, Gly13Asp, Gly12Ala, Gly12Asp, Gly12Val, and Gln61His), the last four of which are pathogenic. In conclusion, here we contribute new genetic variation data and provide clinicopathological information pertinent to CRC in Rwanda.
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- 2023
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33. Lipidomics-based tissue heterogeneity in specimens of luminal breast cancer revealed by clustering analysis of mass spectrometry imaging: A preliminary study.
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Aramaki S, Tsuge S, Islam A, Eto F, Sakamoto T, Oyama S, Li W, Zhang C, Yamaguchi S, Takatsuka D, Hosokawa Y, Waliullah ASM, Takahashi Y, Kikushima K, Sato T, Koizumi K, Ogura H, Kahyo T, Baba S, Shiiya N, Sugimura H, Nakamura K, and Setou M
- Subjects
- Mass Spectrometry, Cluster Analysis, Triglycerides, Lipidomics methods, Neoplasms
- Abstract
Cancer tissues reflect a greater number of pathological characteristics of cancer compared to cancer cells, so the evaluation of cancer tissues can be effective in determining cancer treatment strategies. Mass spectrometry imaging (MSI) can evaluate cancer tissues and even identify molecules while preserving spatial information. Cluster analysis of cancer tissues' MSI data is currently used to evaluate the phenotype heterogeneity of the tissues. Interestingly, it has been reported that phenotype heterogeneity does not always coincide with genotype heterogeneity in HER2-positive breast cancer. We thus investigated the phenotype heterogeneity of luminal breast cancer, which is generally known to have few gene mutations. As a result, we identified phenotype heterogeneity based on lipidomics in luminal breast cancer tissues. Clusters were composed of phosphatidylcholine (PC), triglycerides (TG), phosphatidylethanolamine, sphingomyelin, and ceramide. It was found that mainly the proportion of PC and TG correlated with the proportion of cancer and stroma on HE images. Furthermore, the number of carbons in these lipid class varied from cluster to cluster. This was consistent with the fact that enzymes that synthesize long-chain fatty acids are increased through cancer metabolism. It was then thought that clusters containing PCs with high carbon counts might reflect high malignancy. These results indicate that lipidomics-based phenotype heterogeneity could potentially be used to classify cancer for which genetic analysis alone is insufficient for classification., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Aramaki et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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- 2023
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34. Cholesterol as a Subsidiary Component of Sorbitan Surfactant-Based Aggregates: A Study of Formation, Hydrophobicity, and Estimation of Localization of Embedded Molecules.
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Hayashi K, Ota H, Sugimura H, Shimanouchi T, Iwasaki T, Fujita S, Nakamura H, and Umakoshi H
- Abstract
Aggregates of amphiphilic molecules can be used as drug carriers, for which the properties can be modified by mixing with other molecules such as cholesterol. It is important to understand the effects of such additives on the properties because they directly define the material functions. In this work, we investigated the effect of cholesterol on the formation and hydrophobicity of aggregates of sorbitan surfactants. As cholesterol changed its formation from micelles to vesicles, an increase in hydrophobicity was seen, particularly in the middle regions compared with the shallow and deep regions. We show that this gradual hydrophobicity is related to the localization of the embedded molecules. 4-Hydroxy-TEMPO and 4-carboxy-TEMPO were preferentially localized in the shallow region of the aggregates, whereas 4-PhCO
2 -TEMPO was preferentially localized in the deep region of the vesicle. The localization of molecules depends on their chemical structure. However, the localization of 4-PhCO2 -TEMPO in micelles was not observed, despite the similar hydrophobicity in the hydrophobic region within the aggregates. The localization of embedded molecules was related to other properties, such as molecular mobility.- Published
- 2023
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35. Modification of Grocott's staining procedure with heat treatment and oxidation by periodic acid for mucormycosis in tissue: a method to detect Mucor spp.
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Kamo YK, Igarashi H, and Sugimura H
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- Periodic Acid, Hot Temperature, Staining and Labeling, Mucor, Mucormycosis diagnosis, Mucormycosis drug therapy
- Abstract
The sensitivity of the Grocott-modified Gomori's methenamine-silver nitrate technique for the detection of fungi is sometimes low, especially for Mucor spp. We modified the Grocott technique by replacing chromic acid with periodic acid in the oxidation step. The use of periodic acid instead of chromic acid enhanced the detectability of Mucor spp. in histopathological sections. Other parameters should be assessed with a high number of cases under different conditions. We propose our protocol as one of the options in practice, especially in cases suspected of Mucor spp. infection.
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- 2023
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36. Primary Cilia Are Frequently Present in Small Cell Lung Carcinomas but Not in Non-Small Cell Lung Carcinomas or Lung Carcinoids.
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Shinmura K, Kato H, Kawasaki H, Hariyama T, Yoshimura K, Tsuchiya K, Watanabe H, Ohta I, Asahina E, Sumiyoshi F, Hamada K, Kawanishi Y, Kawase A, Funai K, and Sugimura H
- Subjects
- Humans, Cilia metabolism, Cilia pathology, Hedgehog Proteins, Lung metabolism, Proteins, Carcinoma, Non-Small-Cell Lung, Carcinoma, Small Cell genetics, Carcinoma, Small Cell metabolism, Carcinoma, Small Cell pathology, Lung Neoplasms genetics, Carcinoid Tumor genetics, Carcinoid Tumor metabolism, Carcinoid Tumor pathology, Small Cell Lung Carcinoma, Adenocarcinoma metabolism, Carcinoma, Squamous Cell
- Abstract
Most human malignant neoplasms show loss of primary cilia (PC). However, PC are known to be retained and involved in tumorigenesis in some types of neoplasms. The PC status in lung carcinomas remains largely uninvestigated. In this study, we comprehensively assessed the PC status in lung carcinomas. A total of 492 lung carcinomas, consisting of adenocarcinomas (ACs) (n = 319), squamous cell carcinomas (SCCs) (n = 152), and small cell lung carcinomas (SCLCs) (n = 21), were examined by immunohistochemical analysis using an antibody against ARL13B, a marker of PC. The PC-positive rate was markedly higher in SCLCs (81.0%) than in ACs (1.6%) and SCCs (7.9%). We subsequently performed analyses to characterize the PC-positive lung carcinomas further. PC-positive lung carcinomas were more numerous and had longer PC than normal cells. The presence of PC in these cells was not associated with the phase of the cell cycle. We also found that the PC were retained even in metastases from PC-positive lung carcinomas. Furthermore, the hedgehog signaling pathway was activated in PC-positive lung carcinomas. Because ARL13B immunohistochemistry of lung carcinoids (n = 10) also showed a statistically significantly lower rate (10.0%) of PC positivity than SCLCs, we searched for a gene(s) that might be upregulated in PC-positive SCLCs compared with lung carcinoids, but not in PC-negative carcinomas. This search, and further cell culture experiments, identified HYLS1 as a gene possessing the ability to regulate ciliogenesis in PC-positive lung carcinomas. In conclusion, our findings indicate that PC are frequently present in SCLCs but not in non-SCLCs (ACs and SCCs) or lung carcinoids, and their PC exhibit various specific pathobiological characteristics. This suggests an important link between lung carcinogenesis and PC., (Copyright © 2022 United States & Canadian Academy of Pathology. Published by Elsevier Inc. All rights reserved.)
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- 2023
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37. Hereditary diffuse gastric cancer in a Japanese family with CDH1 mutation three case reports.
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Muranaka F, Kise E, Tokumaru S, Kitazawa M, Miyagawa Y, Suga T, Uehara T, Iwaya M, Kobayashi S, Sato M, Gomi D, Yamada H, Sugimura H, Kosho T, Soejima Y, and Koizumi T
- Abstract
Background: Germline pathogenic variants in the E-cadherin gene CDH1 cause hereditary diffuse gastric cancer (HDGC), which is an autosomal dominant cancer syndrome, accounting for 1-3% of all gastric cancers. HDGC harboring a CDH 1 variant is extremely rare in Japan., Method: In this study we report the clinical courses of three cases with HDGC from a single Japanese family., Results: The proband exhibited advanced and metastatic gastric cancer, and was found to have a previously reported heterozygous frameshift variant in CDH1 (NM_004360.3:c.1009_1010del:p.Ser337Phefs*12). Five at-risk relatives underwent presymptomatic molecular testing after careful genetic counseling, and three were molecularly diagnosed as positive for the variant. Esophagogastroduodenoscopy was performed in these relatives revealing abnormal small pale mucosal patches, small ulcerative lesion and no abnormal findings. Moreover, random and targeted biopsies were compatible with pathological diagnosis of HDGC in the three cases, all of which underwent total prophylactic gastrectomy., Conclusion: It is critical for the assessment and management of HDGC patients to be actively offered a multidisciplinary and familial-oriented approach. Notably, genetic screening in suspected individuals and familial members is a determining piece for a higher detection rate and the identification of clinical relevant mutations in both low and high-incidence gastric cancer countries., (© 2023. The Author(s).)
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- 2023
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38. Lipid biomarkers that reflect postoperative recurrence risk in lung cancer patients who smoke: a case-control study.
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Takanashi Y, Kahyo T, Hayakawa T, Sekihara K, Kawase A, Kondo M, Kitamoto T, Takahashi Y, Sato T, Sugimura H, Shiiya N, Setou M, and Funai K
- Subjects
- Humans, Case-Control Studies, Reproducibility of Results, Biomarkers, Tumor analysis, Smoking adverse effects, Lipids, Lung Neoplasms etiology, Lung Neoplasms surgery, Adenocarcinoma of Lung, Carcinoma, Squamous Cell pathology
- Abstract
Background: The risk of postoperative recurrence is higher in lung cancer patients who smoke than non-smokers. However, objective evaluation of the postoperative recurrence risk is difficult using conventional pathological prognostic factors because of their lack of reproducibility. Consequently, novel objective biomarkers that reflect postoperative risk in lung cancer patients who smoke must be identified. Because cigarette smoking and oncogenesis alter lipid metabolism in lung tissue, we hypothesized that the lipid profiles in lung cancer tissues are influenced by cigarette smoking and can reflect the postoperative recurrence risk in smoking lung cancer patients. This study aimed to identify lipid biomarkers that reflect the smoking status and the postoperative recurrence risk., Methods: Primary tumor tissues of lung adenocarcinoma (ADC) (n = 26) and squamous cell carcinoma (SQCC) (n = 18) obtained from surgery were assigned to subgroups according to the patient's smoking status. The ADC cohort was divided into never smoker and smoker groups, while the SQCC cohort was divided into moderate smoker and heavy smoker groups. Extracted lipids from the tumor tissues were subjected to liquid chromatography-tandem mass spectrometry analysis. Lipids that were influenced by smoking status and reflected postoperative recurrence and pathological prognostic factors were screened., Results: Two and 12 lipid peaks in the ADC and SQCC cohorts showed a significant positive correlation with the Brinkman index, respectively. Among them, in the ADC cohort, a higher lipid level consisted of three phosphatidylcholine (PC) isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), was associated with a shorter recurrence free period (RFP) and a greater likelihoods of progressed T-factor (≥ pT2) and pleural invasion. In the SQCC cohort, a lower m/z 736.5276 level was associated with shorter RFP and greater likelihood of recurrence., Conclusions: From our data, we propose three PC isomers, PC (14:0_18:2), PC (16:1_16:1), and PC (16:0_16:2), and a lipid peak of m/z 736.5276 as novel candidate biomarkers for postoperative recurrence risk in lung ADC and SQCC patients who are smokers., (© 2023. The Author(s).)
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- 2023
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39. Thyroid transcription factor-1 expression in rectal adenocarcinoma metastatic to the lung.
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Takanashi Y, Kurachi K, Fujihiro M, Sekihara K, Torii K, Kawase A, Matsubayashi Y, Hayakawa T, Baba S, Sugimura H, Iwashita T, and Funai K
- Abstract
Distinguishing metastatic lung tumors from primary lung cancer is essential for planning the appropriate treatment strategy. Thyroid transcription factor-1 (TTF-1) is a reliable immunohistochemistry (IHC) marker for differentiating between primary lung adenocarcinomas and metastatic lung tumors originating from colorectal adenocarcinomas. Herein, we report a rare case of TTF-1 expression in both the metastatic lung tumor and primary rectal adenocarcinoma. Aside from the similar histological characteristics of both tumors when stained with hematoxylin-eosin, the IHC patterns, including negative results for alveolar epithelium markers (napsin A and CK7) and positive results for intestinal markers (CK20, CDX2, SATB2, and β-catenin), of the lung tumor and the primary rectal adenocarcinoma strongly supported the final diagnosis. Considering the non-negligible frequency of TTF-1 positivity in colorectal adenocarcinomas, applying the IHC panel including multiple markers for alveolar epithelium and intestinal differentiation, would be helpful to support the diagnosis of metastatic lung tumor from a rectal adenocarcinoma., Competing Interests: None declared., (© 2023 The Authors.)
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- 2023
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40. Non-CpG sites preference in G:C > A:T transition of TP53 in gastric cancer of Eastern Europe (Poland, Romania and Hungary) compared to East Asian countries (China and Japan).
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Natsume H, Szczepaniak K, Yamada H, Iwashita Y, Gędek M, Šuto J, Ishino K, Kasajima R, Matsuda T, Manirakiza F, Nzitakera A, Wu Y, Xiao N, He Q, Guo W, Cai Z, Ohta T, Szekely T, Kadar Z, Sekiyama A, Oshima T, Yoshikawa T, Tsuburaya A, Kurono N, Wang Y, Miyagi Y, Gurzu S, and Sugimura H
- Abstract
Aim: Mutation spectrum of TP53 in gastric cancer (GC) has been investigated world-widely, but a comparison of mutation spectrum among GCs from various regions in the world are still sparsely documented. In order to identify the difference of TP53 mutation spectrum in GCs in Eastern Europe and in East Asia, we sequenced TP53 in GCs from Eastern Europe, Lujiang (China), and Yokohama, Kanagawa (Japan) and identified the feature of TP53 mutations of GC in these regions., Subjects and Method: In total, 689 tissue samples of GC were analyzed: 288 samples from East European populations (25 from Hungary, 71 from Poland and 192 from Romania), 268 from Yokohama, Kanagawa, Japan and 133 from Lujiang, Anhui province, China. DNA was extracted from FFPE tissue of Chinese, East European cases; and from frozen tissue of Japanese GCs. PCR products were direct-sequenced by Sanger method, and in ambiguous cases, PCR product was cloned and up to 8 clones were sequenced. We used No. NC_000017.11(hg38) as the reference sequence of TP53. Mutation patterns were categorized into nine groups: six base substitutions, insertion, deletion and deletion-insertion. Within G:C > A:T mutations the mutations in CpG and non-CpG sites were divided. The Cancer Genome Atlas data (TCGA, ver.R20, July, 2019) having somatic mutation list of GCs from Whites, Asians, and other ethnicities were used as a reference for our data., Results: The most frequent base substitutions were G:C > A:T transition in all the areas investigated. The G:C > A:T transition in non-CpG sites were prominent in East European GCs, compared with Asian ones. Mutation pattern from TCGA data revealed the same trend between GCs from White (TCGA category) vs Asian countries. Chinese and Japanese GCs showed higher ratio of G:C > A:T transition in CpG sites and A:T > G:C mutation was more prevalent in Asian countries., Conclusion: The divergence in mutation spectrum of GC in different areas in the world may reflect various pathogeneses and etiologies of GC, region to region. Diversified mutation spectrum in GC in Eastern Europe may suggest GC in Europe has different carcinogenic pathway of those from Asia., (© 2023. The Author(s).)
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- 2023
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41. Identifying Active Progeny Virus Particles in Formalin-Fixed, Paraffin-Embedded Sections Using Correlative Light and Scanning Electron Microscopy.
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Itoh T, Yamada S, Ohta I, Meguro S, Kosugi I, Iwashita T, Itoh H, Kanayama N, Okudela K, Sugimura H, Misawa K, Hariyama T, and Kawasaki H
- Subjects
- Humans, Microscopy, Electron, Scanning, Paraffin Embedding, 3,3'-Diaminobenzidine, Formaldehyde, Virion
- Abstract
Immunohistochemical analysis of formalin-fixed paraffin-embedded (FFPE) tissue blocks is routinely used to identify virus-infected cells. However, detecting virus particles in FFPE sections using light microscopy is difficult because of the light diffraction resolution limitations of an optical microscope. In this study, light microscopy and field emission scanning electron microscopy were performed to observe 3-dimensional virus particles in FFPE sections in a nondestructive manner using NanoSuit or osmium conductive treatment methods. The virus particles in FFPE sections were immunostained with specific antibodies against the surface antigens of the viral particles and stained with 3,3'-diaminobenzidine. A metal solution (0.2% gold chloride or 2% osmium tetroxide) was applied to enhance the 3,3'-diaminobenzidine-stained area. This procedure is nondestructive for FFPE sections and is a simpler method than transmission electron microscopy. To validate the applicability of this technique, we performed 3-dimensional imaging of the virus particles of different sizes, such as human papillomavirus, cytomegalovirus, and varicella-zoster virus. Furthermore, ultrathin sections from the FFPE sections that were observed to harbor viral particles using field emission scanning electron microscopy were prepared and assessed using transmission electron microscopy. In the correlative areas, transmission electron microscopy confirmed the presence of large numbers of virus particles. These results indicated that the combination of marking viral particles with 3,3'-diaminobenzidine/metal staining and conductive treatment can identify active progeny virus particles in FFPE sections using scanning electron microscopy. This easy correlative imaging of field emission scanning electron microscopy of the identical area of FFPE in light microscopy may help elucidate new pathological mechanisms of virus-related diseases., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2023
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42. Fundamental and higher eigenmodes of qPlus sensors with a long probe for vertical-lateral bimodal atomic force microscopy.
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Yamada Y, Ichii T, Utsunomiya T, Kimura K, Kobayashi K, Yamada H, and Sugimura H
- Abstract
The detection of vertical and lateral forces at the nanoscale by atomic force microscopy (AFM) reveals various mechanical properties on surfaces. The qPlus sensor is a widely used force sensor, which is built from a quartz tuning fork (QTF) and a sharpened metal probe, capable of high-resolution imaging in viscous liquids such as lubricant oils. Although a simultaneous detection technique of vertical and lateral forces by using a qPlus sensor is required in the field of nanotribology, it has still been difficult because the torsional oscillations of QTFs cannot be detected. In this paper, we propose a method to simultaneously detect vertical and lateral force components by using a qPlus sensor with a long probe. The first three eigenmodes of the qPlus sensor with a long probe are theoretically studied by solving a set of equations of motion for the QTF prong and probe. The calculation results were in good agreement with the experimental results. It was found that the tip oscillates laterally in the second and third modes. Finally, we performed friction anisotropy measurements on a polymer film by using a bimodal AFM utilizing the qPlus sensor with a long probe to confirm the lateral force detection., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)
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- 2022
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43. A case of mycosis fungoides with large cell transformation which shows germinal centers preference of lymph node.
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Ishikawa R, Yamada H, Takahashi J, Baba S, Tanioka F, and Sugimura H
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- Humans, Lymph Nodes pathology, Cell Transformation, Neoplastic pathology, Germinal Center pathology, Mycosis Fungoides pathology, Skin Neoplasms pathology
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- 2022
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44. Methylation of CpG island promoters at ZNF625 , LONRF2 , SDC2 and WDR17 in a patient with numerous non-granular type laterally spreading tumors and colorectal cancer: A case report.
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Iwaizumi M, Taniguchi T, Kurachi K, Osawa S, Sugimoto K, Baba S, Sugimura H, and Maekawa M
- Abstract
Patients with adenomatous polyposis syndromes such as familial adenomatous polyposis are at higher risk of colorectal cancer, hence continuous management is necessary. However, little is known about the etiology of patients with numerous laterally spreading tumors (LSTs), or how genetic alterations uniquely influence LSTs in colorectal carcinogenesis. The present case report investigated a woman with >150 non-granular type LSTs (LST-NG) and one sigmoid colon cancer. After subtotal colectomy via ileorectal anastomosis, genetic and epigenetic analyses were conducted by comparing the profiles of the patient's normal colonic mucosa, four LST-NG lesions and a cancer lesion. Using customized multigene panel testing, no pathogenic germline mutations were detected, including APC regulator of WNT signaling pathway, but identified a somatic pathogenic variant of APC in one LST-NG lesion, and both TP53 and F-box and WD repeat domain containing 7 somatic mutations in the cancer. Comprehensive genome-wide methylation analysis showed that CpG island promoters at zinc finger protein 625, LON peptidase N-terminal domain and ring finger 2, WD repeat domain 17 and syndecan 2 were methylated in both LST-NG and cancer, which may contribute to colorectal tumorigenesis as early as the LST-NG phase., Competing Interests: The authors declare that they have no competing interests., (Copyright: © Iwaizumi et al.)
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- 2022
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45. Exogenous Lipoid Pneumonia Caused by Gargling With Sesame Oil: A Case Report.
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Yamada A, Kagawa T, Nishimoto Y, Sugawara R, Arai T, Inoue Y, Taiji R, Okada H, Takewa M, Sugimura H, and Tanaka T
- Subjects
- Humans, Tomography, X-Ray Computed adverse effects, Sesame Oil therapeutic use, Pneumonia, Lipid chemically induced, Pneumonia, Lipid diagnostic imaging
- Abstract
Competing Interests: The authors declare no conflicts of interest.
- Published
- 2022
- Full Text
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46. Recent Advances in the Treatment of Pulmonary Arterial Hypertension.
- Author
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Otani N, Tomoe T, Kawabe A, Sugiyama T, Horie Y, Sugimura H, Yasu T, and Nakamoto T
- Abstract
Pulmonary arterial hypertension (PAH) is a disease in which stenosis or obstruction of the pulmonary arteries (PAs) causes an increase in PA pressure, leading to right-sided heart failure and death. Basic research has revealed a decrease in the levels of endogenous vasodilators, such as prostacyclin, and an increase in the levels of endogenous vasoconstrictors, such as endothelin, in patients with PAH, leading to the development of therapeutic agents. Currently, therapeutic agents for PAH target three pathways that are selective for PAs: the prostacyclin, endothelin, and nitric oxide pathways. These treatments improve the prognosis of PAH patients. In this review, we introduce new drug therapies and provide an overview of the current therapeutic agents., Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
- Published
- 2022
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47. m 6 A demethylase ALKBH5 promotes tumor cell proliferation by destabilizing IGF2BPs target genes and worsens the prognosis of patients with non-small-cell lung cancer.
- Author
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Tsuchiya K, Yoshimura K, Iwashita Y, Inoue Y, Ohta T, Watanabe H, Yamada H, Kawase A, Tanahashi M, Ogawa H, Funai K, Shinmura K, Suda T, and Sugimura H
- Subjects
- Humans, AlkB Homolog 5, RNA Demethylase genetics, AlkB Homolog 5, RNA Demethylase metabolism, Cell Proliferation genetics, Prognosis, RNA, Messenger genetics, RNA-Binding Proteins metabolism, Carcinoma, Non-Small-Cell Lung genetics, Carcinoma, Non-Small-Cell Lung metabolism, Lung Neoplasms pathology
- Abstract
The modification of N
6 -methyladenosine (m6 A) in RNA and its eraser ALKBH5, an m6 A demethylase, play an important role across various steps of human carcinogenesis. However, the involvement of ALKBH5 in non-small-cell lung cancer (NSCLC) development remains to be completely elucidated. The current study revealed that the expression of ALKBH5 was increased in NSCLC and increased expression of ALKBH5 worsened the prognosis of patients with NSCLC. In vitro study revealed that ALKBH5 knockdown suppressed cell proliferation ability of PC9 and A549 cells and promoted G1 arrest and increased the number of apoptotic cells. Furthermore, ALKBH5 overexpression increased the cell proliferation ability of the immortalized cell lines. Microarray analysis and western blotting revealed that the expression of CDKN1A (p21) or TIMP3 was increased by ALKBH5 knockdown. These alterations were offset by a double knockdown of both ALKBH5 and one of the IGF2BPs. The decline of mRNAs was, at least partly, owing to the destabilization of these mRNAs by one of the IGF2BPs. In conclusions, the ALKBH5-IGF2BPs axis promotes cell proliferation and tumorigenicity, which in turn causes the unfavorable prognosis of NSCLC., (© 2022. The Author(s).)- Published
- 2022
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48. The relationship between chest tube position in the thoracic cavity and treatment failure in patients with pleural infection: a retrospective cohort study.
- Author
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Taniguchi J, Nakashima K, Matsui H, Nagai T, Otsuki A, Ito H, and Sugimura H
- Subjects
- Humans, Pleural Cavity, Retrospective Studies, Treatment Failure, Chest Tubes adverse effects, Pleural Diseases
- Abstract
Background: Pleural infection is an infection of the pleural space that is usually treated with antibiotics and source control. Chest tube insertion is the most popular and widely used drainage technique. We typically attempt to place the tube at the bottom of the thoracic cavity to consider the effects of gravity; however, the effectiveness of this practice is not well-defined. Therefore, we aimed to examine whether the position of the tip of the thoracic tube affects treatment failure in patients with pleural infection., Methods: In this retrospective observational study, patients with pleural infection who underwent thoracic tube insertion were divided into two groups: those with the tip of the tube positioned below the 10th thoracic vertebra at the level of the diaphragm (lower position group) and those with the tip placed above the 9th thoracic vertebra (upper position group). We compared whether the position of the tube tip affected treatment failure. Stabilized inverse probability treatment weights (SIPTW) were used to balance the baseline characteristics between the groups. Treatment failure showed a composite outcome of hospital death, referral to surgeons for surgery, and additional chest tube insertion., Results: Among the 87 patients, 41 and 46 patients were in the lower and upper groups, respectively. No significant difference was observed in the composite outcomes between the groups (46.3% vs. 54.3%, P = 0.596). There was also no significant difference in the composite outcome between both groups after adjusting for SIPTW (52.3% vs. 68.8%, P = 0.286)., Conclusions: There were no significant differences in the treatment failure in this study addressing pleural infection treatment, in which the drain tip position was stratified by the 9th and 10th thoracic vertebrae. The position of the tip of the thoracic tube may not be important for pleural infection treatment providing that it is in the thoracic cavity. Trial registration The participants were registered retrospectively., (© 2022. The Author(s).)
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- 2022
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49. Efficacy of HSV-TK/GCV system suicide gene therapy using SHED expressing modified HSV-TK against lung cancer brain metastases.
- Author
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Oishi T, Ito M, Koizumi S, Horikawa M, Yamamoto T, Yamagishi S, Yamasaki T, Sameshima T, Suzuki T, Sugimura H, Namba H, and Kurozumi K
- Abstract
Lung cancer is one of the most common cancers, and the number of patients with intracranial metastases is increasing. Previously, we developed an enzyme prodrug suicide gene therapy based on the herpes simplex virus thymidine kinase (HSV-TK)/ganciclovir (GCV) system using various mesenchymal stem cells to induce apoptosis in malignant gliomas through bystander killing effects. Here, we describe stem cells from human exfoliated deciduous teeth (SHED) as gene vehicles of the TK/GCV system against a brain metastasis model of non-small cell lung cancer (NSCLC). We introduced the A168H mutant TK (TK
A168H ) into SHED to establish the therapeutic cells because of the latent toxicity of wild type. SHED expressing TKA168H (SHED-TK) exhibited chemotaxis to the conditioned medium of NSCLC and migrated toward implanted NSCLC in vivo . SHED-TK demonstrated a strong bystander effect in vitro and in vivo and completely eradicated H1299 NSCLC in the brain. SHED-TK cells implanted intratumorally followed by GCV administration significantly suppressed the growth of H1299 and improved survival time. These results indicate that the TKA168H variant is suitable for establishing therapeutic cells and that intratumoral injection of SHED-TK followed by GCV administration may be a useful strategy for therapeutic approaches., Competing Interests: The authors declare no competing interests., (© 2022 The Authors.)- Published
- 2022
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50. Induction of DNA Damage in Mouse Colorectum by Administration of Colibactin-producing Escherichia coli , Isolated from a Patient With Colorectal Cancer.
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Narita T, Tsunematsu Y, Miyoshi N, Komiya M, Hamoya T, Fujii G, Yoshikawa Y, Sato M, Kawanishi M, Sugimura H, Iwashita Y, Totsuka Y, Terasaki M, Watanabe K, Wakabayashi K, and Mutoh M
- Subjects
- Animals, DNA Damage, Escherichia coli genetics, Female, Humans, Mice, Peptides, Polyketides, Rats, Colorectal Neoplasms genetics, Escherichia coli Infections microbiology
- Abstract
Background/aim: Among colorectal cancer-associated intestinal microbiota, colibactin-producing (clb
+ ) bacteria are attracting attention. We aimed to clarify the interaction between clb+ Escherichia coli and normal colorectal epithelial cells in vivo and in vitro., Materials and Methods: Five-week-old female Balb/c mice were divided in an untreated group, a group treated with clb+ E. coli isolated from a Japanese patient with colorectal cancer (E. coli-50), and a group treated with non colibactin-producing E. coli (E. coli-50/ΔclbP). Mice were sacrificed at 18 weeks of treatment., Results: Treatment with clb+ E. coli increased positivity for H2A histone family member X phosphorylated at Ser-139 (γH2AX) in epithelial cells of the luminal surface of the mouse rectum but this did not occur in the E. coli-50/ΔclbP and untreated groups. In an in vitro setting, the ratio of apoptotic cells was increased and cell counts were reduced by treatment with clb+ E. coli more than in untreated cells and normal rat colorectal epithelial cells., Conclusion: E. coli-50 induced DNA damage in the mouse rectum, possibly by direct interaction between clb+ E. coli and normal colorectal epithelial cells. Our findings imply that regulation of clb+ E. coli infection may be a useful strategy for colorectal cancer control., (Copyright © 2022, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)- Published
- 2022
- Full Text
- View/download PDF
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