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Evaluation of Salinity Tolerance on Five Strains of Nile Tilapia (Oreochromis niloticus).        The objective of the study was to evaluate the salinity tolerance of five strains of nile tilapia, BEST (Bogor Enhanced Strain Tilapia), Lokal Kuningan (LK), Red NIFI (RN), nila merah (NM) dan nila hitam (NH), in 15 ppt saline water. Initial size of fish examined was 3-5 cm, reared in 100 x 40 x 60 cm aquaria with density of 25 fish per aquarium. Each strain had 4 replications. During experiment fish were fed with commercial pellet, 5% of body weigth per day. Observation was done on standard length, body weight, biomass, and survival rate every 10 days for 1 month. Growth of length and body weight showed no differences among strains. For biomass, BEST strain except with RN was significantly different (P<0.01) compare to NH, NM and LK. On the other hand, RN was not significantly different with NH, but significantly different with LK and NM. For survival rate, BEST strain had the highest percentage among others and significantly different than LK, NM and NH. RN was also significantly different than LK, NM, and NH. The last three strains were not significant different among them.Keywords : Strain, oreochromis, salinity, growth ABSTRAK       Banyaknya lahan tambak yang tidak dioperasikan lagi (idle) merupakan lahan tidur yang perlu dioptimalkan Penelitian yang dilakukan bertujuan untuk melakukan evaluasi pertumbuhan benih ikan nila strain BEST (Bogor Enhanced Strain Tilapia), lokal Kuningan, Red NIFI, nila merah dan nila hitam di masyarakat pada media bersalinitas 15 ppt. Ikan uji yang digunakan berukuran 3-5 cm dipelihara dalam akuarium ukuran 100 x 40 x 60 cm dengan kepadatan 25 ekor menggunakan 4 ulangan untuk masing-masing strain. Selama pemeliharaan, ikan diberi pakan komersial (pellet) sebanyak 5% per hari dari bobot tubuh. Pengamatan panjang baku, bobot tubuh, biomasa dan sintasan dilakukan setiap 10 hari sekali selama masa pemeliharaan.

Phenotype Diversity of BEST Nila Fish of F4, F5 and The Nirwana 2 Nila Fish Using Truss Morphometric Analysis         In the framework of the management of genetic resources for long-term development and sustainability of cultivating nila tilapia fish for the evaluation of population genetic resources needs to be done. The purpose of this research was to know the fish of nila tilapia BEST phenotype diversity of F4, F5 and the nila tilapia fish of Nirwana 2 using Truss Morfometrik. The fish from populations of Tilapia BEST fish of F4, F5 and a Nirwana 2 fish each sample taken as many as 20 tails. The measurement was done by specifying points along the body of the fish assay based on morfometrik truss method. The dots were connected one with the others so retrieved 21 characters measuring results. The analys was done using cluster analysis. The observations indicated levels of similarity of truss morfometrik BEST Fish of F4, F5 and the Nirwana 2 fish tilapia very high character except on B6, B3, A3, B1, C1 and D6 that significantly different (p ≤ 0.05).The results gave an indication of the quantity of fish body that BEST fish was  shorter and rounder than the fish of Nirwana 2 tilapia. Based on the inter population relationship the fish of Nirwana 2 tilapia separated  from BEST nila of F4 and F5.Keywords : BEST tilapia, Nirwana tilapia, truss morfometrik, diversity ABSTRAK        Dalam rangka pengelolaan sumber genetik jangka panjang dan pengembangan budidaya untuk kelestarian ikan nila maka evaluasi sumber daya genetik populasi perlu dilakukan.Tujuan dari penelitian ini adalah mengetahui ragam fenotipe ikan nila BEST F4, F5 dan ikan nila Nirwana 2menggunakanTruss Morfometrik. Ikan nila dari populasi nila BEST F4,F5 dan nila Nirwana2 masing-masing diambil contoh sebanyak 20 ekor. Pengukuran dilakukan dengan menentukan titik-titikacuan sepanjang tubuh ikan uji berdasarkanmetode Truss Morfometrik. Titik-titik dihubungkan satu dengan lainnyasehi

Analysis Genetic Variation of Three Strains of Red Tilapia by Anova of RAPD.         Study on the genetic variance of three strains of red tilapia (Oreochromis sp) had been conducted in the Biology Molecular Laboratory, Research Institute for Freshwater Aquaculture (RIFA) Bogor. Three different strains, Red NIFI from Thailand, Red Tilapia from Lido lake and Red Tilapia from Bogor’s farmer were analyzed in the study. Observation used Random Amplified Polymorphism DNA (RAPD) with OPA-03, OPA-04, OPC 14 and OPC-15 primers. The results showed that only OPA-03 primer was able to amplify numerous samples. Further analysis showed that the percentage of polimorphic range was between 16.67 – 38.89%, heterozygosity value 0.0378 – 0.1536 and genetic distance among strain 0.3051 – 0.6037.Keywords : RAPD, genetic, strain, nile tilapia, oreochromis ABSTRAK          Penelitian mengenai variasi genetik tiga strain nila merah dari 3 lokasi yang berbeda telah dilakukan di Laboratorium Molekuler Biologi, Balai Riset Perikanan Budidaya Air Tawar (BRPBAT) Bogor. Strain yang diamati meliputi jenis nila Red NIFI dari Thailand, nila merah dari Danau Lido dan nila merah dari Petani Bogor. Penelitian menggunakan metode analisis Random Amplified Polymorphism DNA (RAPD), dengan menggunakan primer OPA-03, OPA-04, OPC-14 dan OPC-15. Hasil pengamatan menunjukkan hanya OPA-03 yang dapat menghasilkan amplifikasi dalam jumlah sampel yang memadai. Hasil analisis menunjukkan bahwa persentase polimorfik berkisar antara 20.00-40.00%, dengan nilai heterozigositas 0.0604–0.1516 dan jarak genetik antar strain 0.1770-0.4865.  Kata kunci : RAPD, genetik, strain, ikan nila, oreochromis

Genotype performance of Tambakan, Helostoma temminckii (Cuvier, 1829) from Sumatera, Java and Kalimantan Polulation using Random Amplified Polymorphic DNA (RAPD)       Tambakan, kissing gourami (Helostoma temminckii) is well known as a freshwater tropical  species from Southeast Asia. In Indonesia, tambakan is an important commodity. However, total production of tambakan tends to decrease. Therefore, domestication has urgently to be done to solve the problem of Tambakan population. Three different tambakan populations from Sumatera, Java and Kalimantan were observed to find good genetic resources for culture activity. This study aimed to investigate the genetic variation of tambakan especially from West Java, Jambi, and South Kalimantan province using RAPD. The result showed that the highest polymorphism and heterozygosity was from South Kalimantan population among others. The three population observed had the fragment size ranged from 100- 2000 bp. The highest genetic distance was between Sumatera and Kalimantan (0,2877), while the lowest was between Kalimantan and Java (0,1961). Key words: Helostoma temminckii, genetic, heterozigosity, genetic relationship ABSTRAK       Ikan tambakan (Helostoma temminckii) adalah salah satu jenis ikan air tawar yang berasal dari wilayah tropis, tepatnya Asia Tenggara. Di Indonesia ikan tambakan merupakan salah satu ikan komoditas penting. Saat ini, jumlah produksi ikan tambakan cenderung menurun. Sehingga domestikasi sangat diperlukan untuk mengatasi masalah peneurunan populasi tambakan Tiga populasi ikan tambakan dari Sumatera, Jawa dan Kalimantan diobeservasi untuk mendapatkan sumber genetic terbaik yang dapat digunakan untuk kegiatan budidaya.. Penelitian ini bertujuan untuk mengidentifikasi keragaman genetika populasi ikan tambakan Jambi, Jawa Barat dan Kalimantan Selatan menggunakan metode RAPD. Hasil menunjukkan bahwa polimorfisme dan heterosigositas tertinggi terdapat pada populasi ikan tambakan Kalimantan

Chicken Manure Biogas Waste as Protein Source for Patin FryF.        The hight of production cost encourage to search for alternative low price feed. The objectives of the study was to know an appropriate substitution by chicken manure biogas protein for soybean meal protein, and the influence on growth performance and feed efficiency of siames catfish (Pangasianodon hypophthalmus). The treatments of chicken manure biogas protein consisted of control (A = 0 %) and four treatment (B = 8 %, C = 16%, D = 24 % dan E = 32 %). The results showed that best chicken manure biogas protein was found in treatments C (16 %), with survival rate of 100 %, specific growth rate (3,4% bw/day), feed conversion ratio (1,0), retention of energy (30,3 %), protein efficiency ratio (3,6 g/g) and 47,8 % of protein retention. Conclusion showed that the chicken manure biogas protein could be used to substitute for soybean meal with the best result in treatment of feed C = 16 %.Keywords : biogas waste, chicken manure, feed, siames catfish, pangasianodon hypophthalmusABSTRAK        Tingginya biaya produksi yang menyebabkan mencari alternatif agar pakan murah. Penelitian ini bertujuan untuk mencari substitusi yang tepat antara protein limbah biogas kotoran ayam terhadap protein bungkil kedelai, serta pengaruhnya terhadap pertumbuhan dan perbandingan efisiensi pakan benih ikan patin (Pangasianodon hypophthalmus). Perlakuan pakan substitusi protein limbah biogas kotoran ayam terhadap protein bungkil kedelai terdiri atas kontrol (A = 0%) dan 4 perlakuan (B = 8 %, C = 16%, D = 24 % dan E = 32%). Hasil penelitian menunjukan bahwa substitusi terbaik pada protein limbah biogas kotoran ayam terhadap protein bungkil kedelai didapatkan pada perlakuan pakan C = 16 %, dengan nilai sintasan 100 %, laju pertumbuhan spesifik 3,4 % bt/hari, rasio konversi pakan 1,0, retensi energi 30,3 %, rasio efisiensi protein 3,6 g/g dan retensi protein 47,8 %. Dari data dapat ditarik kesimpulan bahwa