Search

Your search keyword '"Gallimore, Awen"' showing total 263 results
Start Over Author "Gallimore, Awen" Publication Year Range Last 10 years
263 results on '"Gallimore, Awen"'

1. Lower Humoral and Cellular Immunity Following Asymptomatic SARS-CoV-2 Infection Compared to Symptomatic Infection in Education (The ACE Cohort)

Author

Hopkins, Georgina, Gomez, Nancy, Tucis, Davis, Bartlett, Laura, Steers, Graham, Burns, Ellie, Brown, Michaela, Harvey-Cowlishaw, Tyler, Santos, Rute, Lauder, Sarah N, Scurr, Martin, Capitani, Lorenzo, Burnell, Stephanie, Rees, Tara, Smart, Kathryn, Somerville, Michelle, Gallimore, Awen, Perera, Marianne, Potts, Martin, Metaxaki, Marina, Krishna, Benjamin, Jackson, Hannah, Tighe, Paddy, Onion, David, Godkin, Andrew, Wills, Mark, and Fairclough, Lucy

Published
2024
Full Text
View/download PDF

3. A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4+ T cell activation

Author

Hulin-Curtis, Sarah, Geary, James K., MacLachlan, Bruce J., Altmann, Danny M., Baillon, Laury, Cole, David K., Greenshields-Watson, Alex, Hesketh, Sophie J., Humphreys, Ian R., Jones, Ian M., Lauder, Sarah N., Mason, Georgina H., Smart, Kathryn, Scourfield, D. Oliver, Scott, Jake, Sukhova, Ksenia, Stanton, Richard J., Wall, Aaron, Rizkallah, Pierre J., Barclay, Wendy S., Gallimore, Awen, and Godkin, Andrew

Published
2024
Full Text
View/download PDF

6. PI3K Isoform Immunotherapy for Solid Tumours

Author

Scott, Jake, Rees, Lauren, Gallimore, Awen, Lauder, Sarah N., Ahmed, Rafi, Series Editor, Akira, Shizuo, Series Editor, Casadevall, Arturo, Series Editor, Galan, Jorge E., Series Editor, Garcia-Sastre, Adolfo, Series Editor, Malissen, Bernard, Series Editor, Rappuoli, Rino, Series Editor, and Dominguez-Villar, Margarita, editor

Published
2022
Full Text
View/download PDF

10. CD4+ T Cells Recognize Conserved Influenza A Epitopes through Shared Patterns of V-Gene Usage and Complementary Biochemical Features

Author

Greenshields-Watson, Alexander, Attaf, Meriem, MacLachlan, Bruce J., Whalley, Thomas, Rius, Cristina, Wall, Aaron, Lloyd, Angharad, Hughes, Hywel, Strange, Kathryn E., Mason, Georgina H., Schauenburg, Andrea J., Hulin-Curtis, Sarah L., Geary, James, Chen, Yuan, Lauder, Sarah N., Smart, Kathryn, Vijaykrishna, Dhanasekaran, Grau, Miguel L., Shugay, Mikhail, Andrews, Robert, Dolton, Garry, Rizkallah, Pierre J., Gallimore, Awen M., Sewell, Andrew K., Godkin, Andrew J., and Cole, David K.

Published
2020
Full Text
View/download PDF

14. Lower Humoral and Cellular Immunity following Asymptomatic SARS-CoV-2 Infection in Education (The ACE Cohort)

Author

Hopkins, Georgina, primary, Gomez, Nancy, additional, Tucis, Davis, additional, Bartlett, Laura, additional, Steers, Graham, additional, Burns, Ellie, additional, Brown, Michaela, additional, Harvey-Cowlishaw, Tyler, additional, Santos, Rute, additional, Lauder, Sarah N, additional, Scurr, Martin, additional, Capitani, Lorenzo, additional, Burnell, Stephanie, additional, Rees, Tara, additional, Smart, Kathryn, additional, Somerville, Michelle, additional, Gallimore, Awen, additional, Perera, Marianne, additional, Potts, Martin, additional, Metaxaki, Marina, additional, Krishna, Benjamin, additional, Jackson, Hannah, additional, Tighe, Paddy, additional, Onion, David, additional, Godkin, Andrew, additional, Wills, Mark, additional, and Fairclough, Lucy, additional

Published
2024
Full Text
View/download PDF

17. TIM3 is a context-dependent co-regulator of cytotoxic T cell function

Author

Alamir, Hanin, primary, Wong, Carissa C.W., additional, Alsubaiti, Amal, additional, Edmunds, Grace L., additional, Grant, Tressan, additional, Alsulaimani, Safaa, additional, Boyd, James, additional, Holland, Christopher J., additional, Morgan, David J., additional, Gallimore, Awen M., additional, and Wülfing, Christoph, additional

Published
2023
Full Text
View/download PDF

18. Peptide mimic for influenza vaccination using nonnatural combinatorial chemistry

Author

Miles, John J., Tan, Mai Ping, Dolton, Garry, Edwards, Emily S.J., Galloway, Sarah A.E., Laugel, Bruno, Clement, Mathew, Makinde, Julia, Ladell, Kristin, Matthews, Katherine K., Watkins, Thomas S., Tungatt, Katie, Wong, Yide, Lee, Han Siean, Clark, Richard J., Pentier, Johanne M., Attaf, Meriem, Lissina, Anya, Ager, Ann, Gallimore, Awen, Rizkallah, Pierre J., Gras, Stephanie, Rossjohn, Jamie, Burrows, Scott R., Cole, David K., Price, David A., and Sewell, Andrew K.

Subjects
Combinatorial chemistry -- Health aspects -- Methods, Synthetic biology -- Research, Influenza vaccines -- Research, Biomimetics -- Health aspects -- Usage, Polypeptides -- Health aspects -- Chemical properties, Pharmacological research, Health care industry
Abstract

Polypeptide vaccines effectively activate human T cells but suffer from poor biological stability, which confines both transport logistics and in vivo therapeutic activity. Synthetic biology has the potential to address these limitations through the generation of highly stable antigenic 'mimics' using subunits that do not exist in the natural world. We developed a platform based on D-amino acid combinatorial chemistry and used this platform to reverse engineer a fully artificial [CD8.sup.+] T cell agonist that mirrored the immunogenicity profile of a native epitope blueprint from influenza virus. This nonnatural peptide was highly stable in human serum and gastric acid, reflecting an intrinsic resistance to physical and enzymatic degradation. In vitro, the synthetic agonist stimulated and expanded an archetypal repertoire of polyfunctional human influenza virus-specific [CD8.sup.+] T cells. In vivo, specific responses were elicited in naive humanized mice by subcutaneous vaccination, conferring protection from subsequent lethal influenza challenge. Moreover, the synthetic agonist was immunogenic after oral administration. This proof-of-concept study highlights the power of synthetic biology to expand the horizons of vaccine design and therapeutic delivery., Introduction [CD8.sup.+] T cells recognize short peptide fragments presented by MHC class I (MHC-I) molecules on the surface of nucleated cells (1-3). These peptide-MHC-I (pMHC-I) molecular arrays are scanned by [...]

Published
2018
Full Text
View/download PDF

20. Figure S5 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells

Author

Milutinovic, Stefan, primary, Abe, Jun, primary, Jones, Emma, primary, Kelch, Inken, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Ware, Carl, primary, Godkin, Andrew, primary, Stein, Jens V., primary, Bogle, Gib, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

21. Supplementary Figures from Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor Rejection

Author

Pires, Ana, primary, Greenshields-Watson, Alexander, primary, Jones, Emma, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Milutinovic, Stefan, primary, Kendrick, Howard, primary, Hindley, James P., primary, French, Rhiannon, primary, Smalley, Matthew J., primary, Watkins, William J., primary, Andrews, Robert, primary, Godkin, Andrew, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

22. Data from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells

Author

Milutinovic, Stefan, primary, Abe, Jun, primary, Jones, Emma, primary, Kelch, Inken, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Ware, Carl, primary, Godkin, Andrew, primary, Stein, Jens V., primary, Bogle, Gib, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

23. Supplementary Movie 2 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells

Author

Milutinovic, Stefan, primary, Abe, Jun, primary, Jones, Emma, primary, Kelch, Inken, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Ware, Carl, primary, Godkin, Andrew, primary, Stein, Jens V., primary, Bogle, Gib, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

24. Supplementary Movie 1 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells

Author

Milutinovic, Stefan, primary, Abe, Jun, primary, Jones, Emma, primary, Kelch, Inken, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Ware, Carl, primary, Godkin, Andrew, primary, Stein, Jens V., primary, Bogle, Gib, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

25. Data from Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor Rejection

Author

Pires, Ana, primary, Greenshields-Watson, Alexander, primary, Jones, Emma, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Milutinovic, Stefan, primary, Kendrick, Howard, primary, Hindley, James P., primary, French, Rhiannon, primary, Smalley, Matthew J., primary, Watkins, William J., primary, Andrews, Robert, primary, Godkin, Andrew, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

26. Supplementary Movie 3 from Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells

Author

Milutinovic, Stefan, primary, Abe, Jun, primary, Jones, Emma, primary, Kelch, Inken, primary, Smart, Kathryn, primary, Lauder, Sarah N., primary, Somerville, Michelle, primary, Ware, Carl, primary, Godkin, Andrew, primary, Stein, Jens V., primary, Bogle, Gib, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

28. Data from Escalating Regulation of 5T4-Specific IFN-γ+ CD4+ T Cells Distinguishes Colorectal Cancer Patients from Healthy Controls and Provides a Target for In Vivo Therapy

Author

Scurr, Martin, primary, Bloom, Anja, primary, Pembroke, Tom, primary, Srinivasan, Rohit, primary, Brown, Clare, primary, Smart, Kathryn, primary, Bridgeman, Hayley, primary, Davies, Mike, primary, Hargest, Rachel, primary, Phillips, Simon, primary, Christian, Adam, primary, Hockey, Tom, primary, Gallimore, Awen, primary, and Godkin, Andrew, primary

Published
2023
Full Text
View/download PDF

30. Supplementary Figure Legend from Escalating Regulation of 5T4-Specific IFN-γ+ CD4+ T Cells Distinguishes Colorectal Cancer Patients from Healthy Controls and Provides a Target for In Vivo Therapy

Author

Scurr, Martin, primary, Bloom, Anja, primary, Pembroke, Tom, primary, Srinivasan, Rohit, primary, Brown, Clare, primary, Smart, Kathryn, primary, Bridgeman, Hayley, primary, Davies, Mike, primary, Hargest, Rachel, primary, Phillips, Simon, primary, Christian, Adam, primary, Hockey, Tom, primary, Gallimore, Awen, primary, and Godkin, Andrew, primary

Published
2023
Full Text
View/download PDF

32. Supplementary Figures 1 - 4 from Escalating Regulation of 5T4-Specific IFN-γ+ CD4+ T Cells Distinguishes Colorectal Cancer Patients from Healthy Controls and Provides a Target for In Vivo Therapy

Author

Scurr, Martin, primary, Bloom, Anja, primary, Pembroke, Tom, primary, Srinivasan, Rohit, primary, Brown, Clare, primary, Smart, Kathryn, primary, Bridgeman, Hayley, primary, Davies, Mike, primary, Hargest, Rachel, primary, Phillips, Simon, primary, Christian, Adam, primary, Hockey, Tom, primary, Gallimore, Awen, primary, and Godkin, Andrew, primary

Published
2023
Full Text
View/download PDF

33. Supplementary Data from Low-Dose Cyclophosphamide Induces Antitumor T-Cell Responses, which Associate with Survival in Metastatic Colorectal Cancer

Author

Scurr, Martin, primary, Pembroke, Tom, primary, Bloom, Anja, primary, Roberts, David, primary, Thomson, Amanda, primary, Smart, Kathryn, primary, Bridgeman, Hayley, primary, Adams, Richard, primary, Brewster, Alison, primary, Jones, Robert, primary, Gwynne, Sarah, primary, Blount, Daniel, primary, Harrop, Richard, primary, Hills, Robert, primary, Gallimore, Awen, primary, and Godkin, Andrew, primary

Published
2023
Full Text
View/download PDF

34. Supplementary Tables from Cancer Antigen Discovery Is Enabled by RNA Sequencing of Highly Purified Malignant and Nonmalignant Cells

Author

Scurr, Martin J., primary, Greenshields-Watson, Alex, primary, Campbell, Emma, primary, Somerville, Michelle S., primary, Chen, Yuan, primary, Hulin-Curtis, Sarah L., primary, Burnell, Stephanie E.A., primary, Davies, James A., primary, Davies, Michael M., primary, Hargest, Rachel, primary, Phillips, Simon, primary, Christian, Adam D., primary, Ashelford, Kevin E., primary, Andrews, Robert, primary, Parker, Alan L., primary, Stanton, Richard J., primary, Gallimore, Awen, primary, and Godkin, Andrew, primary

Published
2023
Full Text
View/download PDF

35. Data from T-Cell Trafficking Facilitated by High Endothelial Venules Is Required for Tumor Control after Regulatory T-Cell Depletion

Author

Hindley, James P., primary, Jones, Emma, primary, Smart, Kathryn, primary, Bridgeman, Hayley, primary, Lauder, Sarah N., primary, Ondondo, Beatrice, primary, Cutting, Scott, primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Withers, David, primary, Price, David A., primary, Ager, Ann, primary, Godkin, Andrew J., primary, and Gallimore, Awen M., primary

Published
2023
Full Text
View/download PDF

36. Supplementary Figures 1-3 from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

37. Data from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

38. Supplementary Figure 6 from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

39. Supplementary Figure 4 from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

40. Supplementary Figure Legends 1-7 from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

41. Supplementary Figure 5 from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

42. Supplementary Figure 7 from Analysis of the T-Cell Receptor Repertoires of Tumor-Infiltrating Conventional and Regulatory T Cells Reveals No Evidence for Conversion in Carcinogen-Induced Tumors

Author

Hindley, James P., primary, Ferreira, Cristina, primary, Jones, Emma, primary, Lauder, Sarah N., primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Betts, Gareth J., primary, Singh, Yogesh, primary, Price, David A., primary, Godkin, Andrew J., primary, Dyson, Julian, primary, and Gallimore, Awen, primary

Published
2023
Full Text
View/download PDF

43. Supplementary Figures 1-6 from T-Cell Trafficking Facilitated by High Endothelial Venules Is Required for Tumor Control after Regulatory T-Cell Depletion

Author

Hindley, James P., primary, Jones, Emma, primary, Smart, Kathryn, primary, Bridgeman, Hayley, primary, Lauder, Sarah N., primary, Ondondo, Beatrice, primary, Cutting, Scott, primary, Ladell, Kristin, primary, Wynn, Katherine K., primary, Withers, David, primary, Price, David A., primary, Ager, Ann, primary, Godkin, Andrew J., primary, and Gallimore, Awen M., primary

Published
2023
Full Text
View/download PDF

44. Oncogenic Properties of Apoptotic Tumor Cells in Aggressive B Cell Lymphoma

Author

Ford, Catriona A., Petrova, Sofia, Pound, John D., Voss, Jorine J.L.P., Melville, Lynsey, Paterson, Margaret, Farnworth, Sarah L., Gallimore, Awen M., Cuff, Simone, Wheadon, Helen, Dobbin, Edwina, Ogden, Carol Anne, Dumitriu, Ingrid E., Dunbar, Donald R., Murray, Paul G., Ruckerl, Dominik, Allen, Judith E., Hume, David A., van Rooijen, Nico, Goodlad, John R., Freeman, Tom C., and Gregory, Christopher D.

Published
2015
Full Text
View/download PDF

45. A Targeted Amino Acid Mutation in Influenza a Virus Universal Epitope Transforms Immunogenicity and Protective Immunity via CD4 T Cell Activation

Author

Hulin-Curtis, Sarah Louise, primary, Geary, James, additional, MacLachlan, Bruce, additional, Altmann, Daniel, additional, Baillon, Laury, additional, Cole, David, additional, Greenshields-Watson, Alexander, additional, Hesketh, Sophie, additional, Humphreys, Ian R., additional, Jones, Ian, additional, Lauder, Sarah, additional, Mason, Georgina, additional, Smart, Kathryn, additional, Scourfield, Oliver, additional, Scott, Jake, additional, Sukhova, Ksenia, additional, Stanton, Richard J., additional, Wall, Aaron, additional, Rizkallah, Pierre, additional, Barclay, Wendy S., additional, Gallimore, Awen, additional, and Godkin, Andrew, additional

Published
2023
Full Text
View/download PDF

47. Three-dimensional Imaging Reveals Immune-driven Tumor-associated High Endothelial Venules as a Key Correlate of Tumor Rejection Following Depletion of Regulatory T Cells

Author

Milutinovic, Stefan, primary, Abe, Jun, additional, Jones, Emma, additional, Kelch, Inken, additional, Smart, Kathryn, additional, Lauder, Sarah N., additional, Somerville, Michelle, additional, Ware, Carl, additional, Godkin, Andrew, additional, Stein, Jens V., additional, Bogle, Gib, additional, and Gallimore, Awen, additional

Published
2022
Full Text
View/download PDF

49. Whole blood-based measurement of SARS-CoV-2-specific T cells reveals asymptomatic infection and vaccine immunogenicity in healthy subjects and patients with solid-organ cancers

Author

Scurr, Martin J, Zelek, Wioleta M, Lippiatt, George, Somerville, Michelle, Burnell, Stephanie EA, Capitani, Lorenzo, Davies, Kate, Lawton, Helen, Tozer, Thomas, Rees, Tara, Roberts, Kerry, Evans, Mererid, Jackson, Amanda, Young, Charlotte, Fairclough, Lucy, Tighe, Paddy, Wills, Mark, Westwell, Andrew D, Morgan, B Paul, Gallimore, Awen, Godkin, Andrew, Scurr, Martin J [0000-0002-4120-0688], Zelek, Wioleta M [0000-0002-2230-3550], Fairclough, Lucy [0000-0003-4581-9741], Gallimore, Awen [0000-0001-6675-7004], Godkin, Andrew [0000-0002-1910-7567], and Apollo - University of Cambridge Repository

Subjects
Adult, Aged, 80 and over, Male, Immunity, Cellular, COVID-19 Vaccines, Adolescent, SARS-CoV-2, Immunology, Vaccination, T cells, COVID-19, Middle Aged, Th1 Cells, Interferon-gamma, Immunogenicity, Vaccine, vaccine, Carrier State, Immunology and Allergy, antibodies, Humans, Female, Aged
Abstract

Accurate assessment of SARS-CoV-2 immunity is critical in evaluating vaccine efficacy and devising public health policies. Whilst the exact nature of effective immunity remains incompletely defined, SARS-CoV-2-specific T-cell responses are a critical feature that will likely form a key correlate of protection against COVID-19. Here, we developed and optimized a high-throughput whole blood-based assay to determine the T-cell response associated with prior SARS-CoV-2 infection and/or vaccination amongst 231 healthy donors and 68 cancer patients. Following overnight in vitro stimulation with SARS-CoV-2-specific peptides, blood plasma samples were analysed for TH 1-type cytokines. Highly significant differential IFN-γ+ /IL-2+ SARS-CoV-2-specific T-cell responses were seen amongst previously infected COVID-19-positive healthy donors in comparison with unknown / naïve individuals (p < 0·0001). IFN-γ production was more effective at identifying asymptomatic donors, demonstrating higher sensitivity (96·0% vs. 83·3%) but lower specificity (84·4% vs. 92·5%) than measurement of IL-2. A single COVID-19 vaccine dose induced IFN-γ and/or IL-2 SARS-CoV-2-specific T-cell responses in 116 of 128 (90·6%) healthy donors, reducing significantly to 27 of 56 (48·2%) when measured in cancer patients (p < 0·0001). A second dose was sufficient to boost T-cell responses in the majority (90·6%) of cancer patients, albeit IFN-γ+ responses were still significantly lower overall than those induced in healthy donors (p = 0·034). Three-month post-vaccination T-cell responses also declined at a faster rate in cancer patients. Overall, this cost-effective standardizable test ensures accurate and comparable assessments of SARS-CoV-2-specific T-cell responses amenable to widespread population immunity testing, and identifies individuals at greater need of booster vaccinations.

Published
2021
Full Text
View/download PDF

50. Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry

Author

Santegoets, Saskia J. A. M., Dijkgraaf, Eveline M., Battaglia, Alessandra, Beckhove, Philipp, Britten, Cedrik M., Gallimore, Awen, Godkin, Andrew, Gouttefangeas, Cecile, de Gruijl, Tanja D., Koenen, Hans J. P. M., Scheffold, Alexander, Shevach, Ethan M., Staats, Janet, Taskén, Kjetil, Whiteside, Theresa L., Kroep, Judith R., Welters, Marij J. P., and van der Burg, Sjoerd H.

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results