171 results on '"Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul"'
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2. Derivation and Validation of the Extended Care (Ex-Care) Model II (Ex-CareII)
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2023
3. Effects of Pilates Standing Exercises on Walking Mobility and Postural Balance
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior., Conselho Nacional de Desenvolvimento Científico e Tecnológico, and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
- Published
- 2021
4. Impact of the 'Ten Steps for Healthy Feeding of Children Younger Than Two Years' in Health Centers
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil, Ministry of Health, Brazil, and Marcia Regina Vitolo, Professor
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- 2021
5. Clinical Impact of COVID-19 by P.1 SARS-CoV-2 Lineage
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil and Alexandre Prehn Zavascki, Principal Investigator
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- 2021
6. Effect of Acetaminophen and N-Acetylcysteine on Liver Metabolism on Homocystinuria
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2021
7. Inflammation and Neurocognitive Damage Markers in Elderly People With Obstructive Sleep Apnea
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2021
8. Convalescent Plasma for Severe COVID-19 Patients (PLACOVID)
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil and Instituto Cultural Floresta
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- 2021
9. Interdisciplinary Intervention With Motivational Approach in Adolescents With Overweight and Obesity. (MERC)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2020
10. Polymorphisms in CD36 and STAT3 Genes and Different Dietary Interventions Among Patients With Coronary Artery Disease (GENUTRI)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2020
11. Food and Nutrition Education in Quilombolas Communities With Food Insecurity: Multi-sector Intervention
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Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil, and Marilda Borges Neutzling, Professor
- Published
- 2019
12. Post-discharge Monitoring of Patients With Heart Failure (CARDIOENF)
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil and Irmandade Santa Casa de Misericórdia de Porto Alegre
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- 2018
13. Brain Response to Single Dose of Pregabalin in Fibromyalgia
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Conselho Nacional de Desenvolvimento Científico e Tecnológico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior., and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2018
14. Effects of Combined Treatment With tDCS and Cognitive Training in Patients With Fibromyalgia
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Conselho Nacional de Desenvolvimento Científico e Tecnológico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior., and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil
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- 2017
15. Combined and Isolated Effects of Sleep Deprivation and Alcohol Intake on Exercise Performance in Humans.
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior., and Marco Aurélio Vaz, PhD, PhD
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- 2016
16. The Effects of Inspiratory Muscle Training in Patients With Heart Failure and Obstructive Sleep Apnea Syndrome
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil and Conselho Nacional de Desenvolvimento Científico e Tecnológico
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- 2016
17. ORal ApplianCes for sLeep Apnea of the Elderly (ORACLE)
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, and Denis Martinez, MD, PhD
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- 2016
18. Treatment of Periodontal Disease in Patients With Acute Myocardial Infarction (TPAMI)
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil and Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Published
- 2015
19. Functional Electrical Stimulation in Chronic Kidney Disease
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Irmandade Santa Casa de Misericórdia de Porto Alegre, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Brazil, and Rodrigo Della Méa Plentz, PhD
- Published
- 2015
20. Effect of Support Matrix and Crosslinking Agents on Nutritional Properties of Orange Juice during Enzyme Clarification: A Comparative Study
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Consejo Nacional de Humanidades, Ciencias y Tecnologías (México), Fernández-Lafuente, Roberto [0000-0003-4976-7096], Dal Magro, Lucas [0000-0002-2463-1021], Rodrigues, Rafael C. [0000-0001-8592-850X], Silva, Pâmela M. da, Esparza-Flores, Eli Emanuel, Virgili, Anike H., Menezes, Eliana W. de, Fernández-Lafuente, Roberto, Dal Magro, Lucas, Rodrigues, Rafael C., Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Ciencia e Innovación (España), Agencia Estatal de Investigación (España), Consejo Nacional de Humanidades, Ciencias y Tecnologías (México), Fernández-Lafuente, Roberto [0000-0003-4976-7096], Dal Magro, Lucas [0000-0002-2463-1021], Rodrigues, Rafael C. [0000-0001-8592-850X], Silva, Pâmela M. da, Esparza-Flores, Eli Emanuel, Virgili, Anike H., Menezes, Eliana W. de, Fernández-Lafuente, Roberto, Dal Magro, Lucas, and Rodrigues, Rafael C.
- Abstract
This study investigated the impact of a support matrix and active group on the support to the nutritional properties of orange juice after juice clarification. Pectinase was immobilized on chitosan and aminated silica supports, activated with genipin or glutaraldehyde, and applied for juice clarification. The effects on various juice properties, including reducing sugars, total soluble solids, vitamin C, and phenolic compounds, juice color, and pH, were evaluated. The results revealed that the immobilization on chitosan activated using genipin resulted in the highest biocatalyst activity (1211.21 U·g−1). The juice treatments using the biocatalysts led to turbidity reduction in the juice (up to 90%), with the highest reductions observed in treatments involving immobilized enzyme on chitosan. Importantly, the enzymatic treatments preserved the natural sugar content, total soluble solids, and pH of the juice. Color differences between treated and raw juice samples were especially relevant for those treated using enzymes, with significant differences in L* and b*, showing loss of yellow vivid color. Analysis of phenolic compounds and vitamin C showed no significant alterations after the enzymatic treatment of the raw juice. According to our results, the clarification of orange juice using immobilized enzymes can be a compromise in turbidity reduction and color reduction to maintain juice quality.
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- 2023
21. Collaboration strategies and corresponding authorship in Agronomy research of Brazilian academic and non‑academic institutions
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Xunta de Galicia, Ministerio de Ciencia e Innovación (España), https://orcid.org/0000-0003-0549-4567, https://orcid.org/0000-0002-8003-0386, https://orcid.org/0000-0002-0968-0108, https://orcid.org/0000-0002-1608-4478, https://orcid.org/0000-0001-6864-1232, de Souza Vanz, Samile Andréa, Gracio, Maria Claudia Cabrini, de Oliveira, Sandra Cristina, Chinchilla-Rodríguez, Zaida, Docampo, Domingo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Xunta de Galicia, Ministerio de Ciencia e Innovación (España), https://orcid.org/0000-0003-0549-4567, https://orcid.org/0000-0002-8003-0386, https://orcid.org/0000-0002-0968-0108, https://orcid.org/0000-0002-1608-4478, https://orcid.org/0000-0001-6864-1232, de Souza Vanz, Samile Andréa, Gracio, Maria Claudia Cabrini, de Oliveira, Sandra Cristina, Chinchilla-Rodríguez, Zaida, and Docampo, Domingo
- Abstract
Agricultural Sciences have become one of Brazil’s most efficient and sustainable research areas, where most publications benefit from scientific collaboration. The present study analyzes the influence of the designation as the corresponding author to researchers linked to Brazilian institutions on the impact of co-authored articles in the category Agronomy from the Web of Science for five-year periods from 2005 to 2019. We have observed a noticeable growth in the number of documents in collaboration between the five-year periods. However, international partnership in the area still needs to be improved, although it is higher in non-academic institutions. The study verified that the documents published by Brazilian authors with international colleagues present a higher normalized citation impact in all types of institutions and five-year periods than documents with national collaboration. The total number of articles, their normalized citation impact, and the percentage of documents as the corresponding author of academic institutions presented less variation than non-academic institutions. For Brazilian academic institutions, assuming the role of the corresponding author of co-authored articles in Agronomy has little influence on citation impact. As for non-academic institutions, we found an association between the corresponding authorship position within the scope of international collaboration and the higher impact of their scientific production. We point out some potential implications and briefly describe the limitations and future research.
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- 2023
22. Stress response of Rhamdia quelen to the interaction stocking density - Feeding regimen
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Baldisserotto, Bernardo [0000-0002-8770-0100], Silva, Hugo Napoleão Pereira da, Almeida, Ana Paula G., Souza, Carine F., Mancera, Juan Miguel, Martos-Sitcha, Juan Antonio, Martínez-Rodríguez, Gonzalo, Baldisserotto, Bernardo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Baldisserotto, Bernardo [0000-0002-8770-0100], Silva, Hugo Napoleão Pereira da, Almeida, Ana Paula G., Souza, Carine F., Mancera, Juan Miguel, Martos-Sitcha, Juan Antonio, Martínez-Rodríguez, Gonzalo, and Baldisserotto, Bernardo
- Abstract
This study aimed to verify the effect of different feeding and stocking conditions during 14 days on the gene expression of several hormones and enzymes related to the stress cascade and metabolic parameters in silver catfish Rhamdia quelen under the following experimental conditions: 1) fed at low stocking density (2.5 kg m-3, LSD-F); 2) fed at high stocking density (32 kg m-3, HSD-F); 3) food-deprived at LSD (LSD-FD); and 4) food-deprived at HSD (HSD-FD). Fish from LSD-F and HSD-F groups were fed daily (1 % of their body mass), while fish from food-deprived groups (LSD-FD and HSD-FD) were not fed during the experimental time. Plasma metabolic parameters (glucose, lactate, triglycerides, and proteins) and hepatosomatic index (HSI) were evaluated. In addition, mRNA expression of genes related to the stress axis (crh, pomca, pomcb, nr3c2, star, hsd11b2 and hsd20b), heat shock protein family (hsp90 and hspa12a), sodium-dependent noradrenaline transporter (slc6a2), and growth axis (gh and igf1) were also assessed. Specific growth rate and HSI decreased in food-deprived fish regardless of stocking density. The HSD-FD group showed weight loss compared to the HSD-F, LSD-F, and LSD-FD groups. Plasma glucose and triglycerides were reduced in food-deprived groups, while lactate and protein levels did not change. The expression of key players of the stress response (crh, pomca, pomcb, hsd11b2, nr3c2, and hsp90b) and growth (gh and igf1) pathways were differently regulated depending on the experimental condition, whereas no statistical difference between treatments was found for hsd20b, scl6a2, hspa12a, and star mRNAs expression. This study suggests that LSD acts as a stressor affecting negatively the physiological status of fed fish, as demonstrated by the reduction in growth rates, altered metabolic orchestration, and a higher crh mRNA expression. In addition, food deprivation also increased mRNA expression of other assessed genes (nr3c2, hsp90b, pomca, and pomcb) in fish fr
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- 2023
23. QCD equation of state at finite isospin density from the linear sigma model with quarks: The cold case
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SCOAP, Universidad Nacional Autónoma de México, Alexander von Humboldt Foundation, Consejo Nacional de Ciencia y Tecnología (México), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), German Research Foundation, Ayala, Alejandro, Bandyopadhyay, Aritra, Farias, Ricardo L. S., Hernández, Luis Alberto, Hernández, José Luis, SCOAP, Universidad Nacional Autónoma de México, Alexander von Humboldt Foundation, Consejo Nacional de Ciencia y Tecnología (México), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), German Research Foundation, Ayala, Alejandro, Bandyopadhyay, Aritra, Farias, Ricardo L. S., Hernández, Luis Alberto, and Hernández, José Luis
- Abstract
We use the two-flavor Linear Sigma Model with quarks to study the phase structure of isospin asymmetric matter at zero temperature. The meson degrees of freedom provide the mean field chiral and isospin condensates on top of which we compute the effective potential accounting for constituent quark fluctuations at one-loop order. Using the renormalizability of the model, we absorb the ultraviolet divergences into suitable counterterms that are added respecting the original structure of the theory. These counterterms are determined from the stability conditions which require the effective potential to have minima in the condensates directions at the classical values, as well as the transition from the noncondensed to the condensed phase to be smooth as a function of the isospin chemical potential. We use the model to study the evolution of the condensates as well as the pressure, energy and isospin densities and the sound velocity as functions of the isospin chemical potential. The approach does a good average description up to isospin chemical potentials values not too large as compared to the vacuum pion mass.
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- 2023
24. Cross-stress memory: Salt priming at vegetative growth stages improves tolerance to drought stress during grain-filling in rice plants
- Author
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Rossatto, Tatiana [0000-0002-8021-5567], Souza, Gustavo Maia [0000-0001-6754-4860], do Amaral, Marcelo Nogueira [0000-0003-3180-7803], Auler, Priscila Ariane [0000-0001-9045-7626], Pérez-Alonso, Marta Marina [0000-0003-4118-4822], Pollmann, Stephan [0000-0002-5111-4425], Braga, Eugenia Jacira Bolacel [0000-0001-6364-7934], Rossatto, Tatiana, Souza, Gustavo Maia, do Amaral, Marcelo Nogueira, Auler, Priscila Ariane, Pérez-Alonso, Marta-Marina, Pollmann, Stephan, Braga, Eugenia Jacira Bolacel, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Rossatto, Tatiana [0000-0002-8021-5567], Souza, Gustavo Maia [0000-0001-6754-4860], do Amaral, Marcelo Nogueira [0000-0003-3180-7803], Auler, Priscila Ariane [0000-0001-9045-7626], Pérez-Alonso, Marta Marina [0000-0003-4118-4822], Pollmann, Stephan [0000-0002-5111-4425], Braga, Eugenia Jacira Bolacel [0000-0001-6364-7934], Rossatto, Tatiana, Souza, Gustavo Maia, do Amaral, Marcelo Nogueira, Auler, Priscila Ariane, Pérez-Alonso, Marta-Marina, Pollmann, Stephan, and Braga, Eugenia Jacira Bolacel
- Abstract
The pre-exposure of plants to a potentially stressful condition can stimulate tolerance to subsequent stresses, a phenomenon associated with memory in plants. When the stimuli have different natures, a cross-tolerance phenomenon can take place. The objective of this study was to investigate whether the pre-exposure of rice plants to a saline stimulus at the vegetative stage has the potential to mitigate the effects of a drought event during grain-filling. By evaluating the transcriptional and physiological (e.g. hormones, gas exchange, growth parameters) responses, this study seek to identify likely long-term memory mechanisms. Therefore, rice plants were exposed to a pre-treatment in the vegetative stage induced by 150 mM NaCl solution for four days and then subjected to 16 days of water deficit in the reproductive stage. Physiological parameters were evaluated and the endogenous levels of the plant hormones abscisic acid (ABA), salicylic acid (SA) and jasmonic acid (JA) and RNA-sequencing (RNA-Seq) was measured to investigate transcriptional profiles of rice plants subjected to cross-stress. The results demonstrated that primed plants challenged by water stress during the grain-filling stage showed less electrolyte leakage, less damage to the photosynthetic apparatus and increased root growth. The higher ABA concentration in leaves was closely related to the above physiological responses. Along with JA and SA, ABA can be a determinant of long-term stress memory in rice. The annotation of specific cross-tolerance genes pointed to signaling and synthesis of hormones, photosynthesis, stomatal conductance, antioxidant system, glyoxylate cycle and transcription factors. It is concluded that priming with salt in the vegetative stage can mitigates the effects of drought during the reproductive stage at the transcriptional, physiological and hormonal levels.
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- 2023
25. A review on amaranth protein: composition, digestibility, health benefits and food industry utilisation
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Egea, Isabel [0000-0002-1248-0297], Rivero Meza, Silvia Leticia, Hirsch Ramos, Adriano, Cañizares, Lázaro, Raphaelli, Chirle de Oliveria, Bueno Peres, Betina, Gaioso, César Augusto, Egea, Isabel, Estrada, Yanira, Flores, Francisco Borja, de Oliveira, Mauricio, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Egea, Isabel [0000-0002-1248-0297], Rivero Meza, Silvia Leticia, Hirsch Ramos, Adriano, Cañizares, Lázaro, Raphaelli, Chirle de Oliveria, Bueno Peres, Betina, Gaioso, César Augusto, Egea, Isabel, Estrada, Yanira, Flores, Francisco Borja, and de Oliveira, Mauricio
- Abstract
Amaranth grains are a source of proteins such as albumins and globulins with well-balanced amino acids profile since they are rich in lysine and methionine, which are scarce in other cereals. Amaranth proteins also show a great bioavailability that contributes to their high quality and, consequently, become a potential substitute for animal-based foods. This review aims to provide a comprehensive summary of the recent research on screening of amaranth proteins as well as their isolation, fractionation, amino acid composition and digestibility. In addition, we carried out an important overview of the nutritional benefits of amaranth proteins and its application as ingredients in food systems. The amaranth has an excellent balance of amino acids and bioactive peptides, with potential health benefits. The consumption of amaranth proteins can promote human health due to their potential to reduce the risk of suffering chronic diseases, while their bioactive peptides play a fundamental role as antioxidant, anti-inflammatory, anti-hypertensive and antimicrobial activities. This review makes an important contribution and approaches the potentiality of using amaranth as a plant protein source, addresses its digestibility and the beneficial health effects brought mainly by the bioactive peptides present in grains
- Published
- 2023
26. Dietary egg white hydrolysate prevents male reproductive dysfunction after long-term exposure to aluminum in rats
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Universidade Federal do Pampa, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Silveira Martinez, Caroline, Uranga-Ocio, José Antonio, Peçanha, Franck Maciel, Vassallo, Dalton Valentim, Exley, Christopher, Miguel, Marta, Wiggers, Giulia Alessandra, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Universidade Federal do Pampa, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Silveira Martinez, Caroline, Uranga-Ocio, José Antonio, Peçanha, Franck Maciel, Vassallo, Dalton Valentim, Exley, Christopher, Miguel, Marta, and Wiggers, Giulia Alessandra
- Abstract
Aluminum (Al) is a non-essential metal omnipresent in human life and is considered an environmental toxicant. Al increases reactive oxygen production and triggers immune responses, contributing to chronic systemic inflammation development. Here, we have tested whether an egg white hydrolysate (EWH) with potential bioactive properties can protect against changes in reproductive function in rats exposed to long-term Al dietary levels at high and low doses. Male Wistar rats received orally: low aluminum level group—AlCl3 at 8.3 mg/kg b.w. for 60 days with or without EWH (1 g/kg/day); high aluminum level group—AlCl3 at 100 mg/kg b.w. for 42 days with or without EWH (1 g/kg/day). The co-administration of EWH prevented the increased Al deposition surrounding the germinative cells, reducing inflammation and oxidative stress in the reproductive organs. Furthermore, the daily supplementation with EWH maintained sperm production and sperm quality similar to those found in control animals, even after Al exposure at a high dietary contamination level. Altogether, our results suggest that EWH could be used as a protective agent against impairment in the reproductive system produced after long-term exposure to Al at low or high human dietary levels.
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- 2022
27. Expression of Ion Transporters and Na+/K+-ATPase and H+-ATPase Activities in the Gills and Kidney of Silver Catfish (Rhamdia quelen) Exposed to Different pHs
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Streck, Morgana Tais, Souza, Carine F., Gottlieb Almeida, A. P., Descovi, Sharine N., Bianchini, Adriane Erbice, Martos-Sitcha, Juan Antonio, Martínez-Rodríguez, Gonzalo, Antoniazzi, Alfredo, Baldisserotto, Bernardo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Streck, Morgana Tais, Souza, Carine F., Gottlieb Almeida, A. P., Descovi, Sharine N., Bianchini, Adriane Erbice, Martos-Sitcha, Juan Antonio, Martínez-Rodríguez, Gonzalo, Antoniazzi, Alfredo, and Baldisserotto, Bernardo
- Abstract
Exposure to acidic and alkaline pHs results in an ionic imbalance. Cellular responses involved in osmoregulation in silver catfish exposed to different pHs (5.5, 7.5, and 9.0) for 24 h were evaluated. The gills and kidney were collected to measure Na+/K+-ATPase (NKA) and H+-ATPase (V-ATPase) activities and to evaluate the expression of ion transporter-related genes: NKA (atp1a1), H+-ATPases (atp6v0a1b, atp6v0a2a, atp6v0a2b), Na+/H+ antiporter (slc9a3), K+/Cl− symporters (slc12a4, slc12a6, slc12a7a, slc12a7b), Na+/K+/2Cl− symporter (slc12a2), and ammonium transporter Rh type b (rhbg). The gills presented greater responses to pH changes than the kidney. The pH alterations changed the atp1a1 gene expression and NKA activity, whereas the H+-ATPase activity increased in the gills in alkaline water, probably to maintain ionic balance. The slc9a3 and slc12a2 genes play more prominent roles in the ion uptake at acidic pH than H+-ATPase. The slc12a7a was the only isoform of this transporter affected by pH. The rhbg is apparently related to ammonia excretion through the gills and kidney (minor scale). Exposure to alkaline pH seems to be battled by impairment of NKA and H+-ATPase activities in the gills, whereas the expression of some ion transporters in silver catfish changes during both acidic and alkaline pHs.
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- 2022
28. Multi-functional egg white hydrolysate prevent hypertension and vascular dysfunction induced by cadmium in rats
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Universidade Federal do Pampa, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Zambelli Moraes, Paola, Gomes Pinheiro, José Eudes, Silveira Martinez, Caroline, Rodrigues Moro, Camila, Silva, Grazielly Caroline da, Diaz Rodriguez, Marina, Ronacher Simões, Maylla, Barbosa Junior, Fernando, Peçanha, Franck Maciel, Vassallo, Dalton Valentim, Miguel, Marta, Wiggers, Giulia Alessandra, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Universidade Federal do Pampa, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Zambelli Moraes, Paola, Gomes Pinheiro, José Eudes, Silveira Martinez, Caroline, Rodrigues Moro, Camila, Silva, Grazielly Caroline da, Diaz Rodriguez, Marina, Ronacher Simões, Maylla, Barbosa Junior, Fernando, Peçanha, Franck Maciel, Vassallo, Dalton Valentim, Miguel, Marta, and Wiggers, Giulia Alessandra
- Abstract
We have investigated if EWH could counteract or prevent cardiovascular damage induced by high level of Cd exposure in rats. Male Wistar rats were treated for 14 days with: (A) Untreated - intraperitoneal (i.p.) injections of distilled water and tap water by gavage; (B) Cd − 1 mg/kg of bw/day of CdCl2 (i.p.) and tap water by gavage; (C) EWH – distilled water (i.p.) and 1 mg/kg/day of EWH by gavage; (D) CdEWH – both treatments. EWH prevented the increase on systolic blood pressure, vascular dysfunction, and inflammation after Cd exposure; prevent the activation of cyclooxygenase (COX)-2 and its derived contractile protanoids, inhibits angiotensin II by the reduction of ACE activity and prevents the increased oxidative stress mainly mediated by NADPH oxidase. Multifunctional EWH could be considered as a natural alternative therapy to counteract the deleterious effects caused by high level of Cd exposure.
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- 2022
29. Transforming second-generation ethanol production waste into sustainable enzymatic cosmetic inputs
- Author
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Polesso, Camila Borges [0000-0002-2423-2741], Rencoret, Jorge [0000-0003-2728-7331], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Camassola, Marli [0000-0001-7410-4337], Polesso, Camila Borges, Fontana, Roselei Claudete, Costa, Cristiana, Fernandes, Bruno, Matamá, Teresa, Cavaco-Paulo, Artur, Rencoret, Jorge, Gutiérrez Suárez, Ana, Schneider, Willian Daniel Hahn, Camassola, Marli, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Polesso, Camila Borges [0000-0002-2423-2741], Rencoret, Jorge [0000-0003-2728-7331], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Camassola, Marli [0000-0001-7410-4337], Polesso, Camila Borges, Fontana, Roselei Claudete, Costa, Cristiana, Fernandes, Bruno, Matamá, Teresa, Cavaco-Paulo, Artur, Rencoret, Jorge, Gutiérrez Suárez, Ana, Schneider, Willian Daniel Hahn, and Camassola, Marli
- Abstract
Agro-industrial residues were used as a successful, cheap and renewable substrate, capable of increasing activity of ligninolytic enzymes for further use in melanin degradation assays to develop inputs for the treatment of skin hyperpigmentation. The analysis of melanin degradation was performed in a quantitative and qualitative way. The enzymatic crude broth extract, rich in laccases, produced by Marasmiellus palmivorus VE111 in medium formulated with lignocellulosic residues of Araucaria angustifolia subjected to hydrolysis and alcoholic fermentation, degraded almost 33% of the melanin content and enzymatic crude broth extract produced by Pycnoporus sanguineus PR_32 degraded 13%, in just 8 h, using vanillin as a mediator. Proton 2D NMR spectra show melanin changes after laccase treatment. This work has provided an insight for the development of ecofriendly, sustainable and innovative cosmetics to deal with skin hyperpigmentation. © 2022 Elsevier B.V.
- Published
- 2022
30. Effects of defoliation frequencies on above- and belowground biodiversity and ecosystem processes in subtropical grasslands of southern Brazil
- Author
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministério da Educação (Brasil), Claudia S. Jorge, Bruna, Fischer, Felícia M., Debastiani, Vanderlei J., Hoss, Daniela, Pillar, Valério D., Winck, Bruna, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Ministério da Educação (Brasil), Claudia S. Jorge, Bruna, Fischer, Felícia M., Debastiani, Vanderlei J., Hoss, Daniela, Pillar, Valério D., and Winck, Bruna
- Abstract
The links between vegetation and soil biota are responsible for a variety of ecosystem processes and services that can be affected by grazing. In this study, we aimed to evaluate the effects of grazing frequency in natural grasslands’ biodiversity (plant and collembola communities) and on ecosystem processes (decomposition and biomass accumulation). For so, we carried out a clipping experiment over a natural area of subtropical grassland in southern Brazil. The plots were submitted during three years to three different clipping frequencies (high, intermediate, and low) to simulate defoliation caused by grazing. We found the highest aboveground biomass accumulation and detritivore activity in the low defoliation frequency, which also promoted the dominance of tussocks. The synergies between higher vegetation height and biomass tend to provide microclimatic conditions that favors decomposition, which releases nutrients to plants, promoting their growth. The defoliation frequencies applied in this study were not sufficient to provide effects on plant community, since its co-evolutionary history with grazing. The Collembola community was not affected by defoliation. It seems to be more influenced by other factors associated with grazing.
- Published
- 2022
31. PANDEMIA DE COVID-19 E MONITORAMENTO ELETRÔNICO: UM ESTUDO DE CASO SOBRE A ATUAÇÃO DO TRIBUNAL DE JUSTIÇA DO RIO GRANDE DO SUL
- Author
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), de Castro, André Giovane, Mori, Emanuele Dallabrida, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), de Castro, André Giovane, and Mori, Emanuele Dallabrida
- Abstract
A pandemia de Covid-19 desafia os controles sanitário e securitário. Este trabalho acadêmico objetiva analisar o sistema carcerário brasileiro à luz dos direitos humanos e da violência, bem como refletir a adoção do monitoramento eletrônico, com o intuito de conter a disseminação do vírus, no âmbito do Tribunal de Justiça do Rio Grande do Sul. A partir da metodologia do estudo de caso, considerando a seleção e o exame de jurisprudência, observou-se a resistência à utilização da tornozeleira eletrônica e a necessidade de contestar a racionalidade punitiva.
- Published
- 2021
32. Yogurt fortification by the addition of microencapsulated stripped weakfish (Cynoscion guatucupa) protein hydrolysate
- Author
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Universidade Federal do Rio Grande do Sul, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Oliveira Lima, Karina, Rocha, Meritaine da, Alemán, Ailén, López Caballero, M. E., Tovar, C. A., Gómez Guillén, M. C., Montero García, Pilar, Prentice, Carlos, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Universidade Federal do Rio Grande do Sul, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Oliveira Lima, Karina, Rocha, Meritaine da, Alemán, Ailén, López Caballero, M. E., Tovar, C. A., Gómez Guillén, M. C., Montero García, Pilar, and Prentice, Carlos
- Abstract
The aim of the present work was to fortify yogurt by adding a stripped weakfish (Cynoscion guatucupa) protein hydrolysate obtained with the enzyme Protamex and microencapsulated by spray drying, using maltodextrin (MD) as wall material. The effects on the physicochemical properties, syneresis, texture, viscoelasticity, antioxidant and ACE inhibitory activities of yogurt after 1 and 7 days of storage were evaluated. In addition, microbiological and sensory analyses were performed. Four yogurt formulations were prepared: control yogurt (without additives, YC), yogurt with MD (2.1%, YMD), with the free hydrolysate (1.4%, YH) and the microencapsulated hydrolysate (3.5%, YHEn). Yogurts to which free and microencapsulated hydrolysates were added presented similar characteristics, such as a slight reduction in pH and increased acidity, with a greater tendency to present a yellow color compared with the control yogurt. Moreover, they showed less syneresis, the lowest value being that of YHEn, which also showed a slight increase in cohesiveness and greater rheological stability after one week of storage. All yogurts showed high counts of the microorganisms used as starters. The hydrolysate presence in both forms resulted in yogurts with antioxidant activity and potent ACE-inhibitory activity, which were maintained after 7 days of storage. The incorporation of the hydrolysate in the microencapsulated form presented greater advantages than the direct incorporation, since encapsulation masked the fishy flavor of the hydrolysate, resulting in stable and sensorily acceptable yogurts with antioxidant and ACE inhibitory activities.
- Published
- 2021
33. Chronopotentiometric study of the transport of phosphoric acid anions through an anion-exchange membrane under different pH values
- Author
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Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear, European Commission, Financiadora de Estudos e Projetos, Brasil, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, CYTED Ciencia y Tecnología para el Desarrollo, Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, Gally, C., García Gabaldón, Montserrat, Ortega Navarro, Emma María, Bernardes, A.M., Pérez-Herranz, Valentín, Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear, European Commission, Financiadora de Estudos e Projetos, Brasil, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, CYTED Ciencia y Tecnología para el Desarrollo, Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, Gally, C., García Gabaldón, Montserrat, Ortega Navarro, Emma María, Bernardes, A.M., and Pérez-Herranz, Valentín
- Abstract
[EN] Phosphate is the main cause of eutrophication in many water bodies. Its presence in waters is associated to the fact that is not completely removed in conventional wastewater treatment plants. On the other side, phosphate rocks are a non-renewable resource and considered as a critical raw material. A membrane separation process, able to recover phosphate from wastewater, is a promising process to avoid pollution and to reuse phosphate. This paper investigates the transport of salts of phosphoric acid through an anion-exchange membrane (AEM) by means of chronopotentiograms and polarization curves (CVCs). The presence of multiple transition times in the chronopotentiograms and the corresponding limiting current densities in the CVCs indicate a change in the species being transported in the membrane/diffusion boundary layer system, due to the hydrolysis reactions that take place when the concentration polarization is reached. Under the experimental conditions tested, coupled convection (gravitational and elctroconvection) occurs when a certain threshold in the membrane voltage drop is surpassed independently of the electrolyte concentration. However, at high pH values, only one transition time in the chronopotentiograms, due to the transfer of OH- ions with greater concentration and mobility. This fact is reflected in the CVCs by the large plateaus obtained, which hinders the occurrence of coupled convection phenomena, and consequently, water splitting can be considered as the main mechanism responsible for the overlimiting regime.
- Published
- 2020
34. DO DIREITO À SAÚDE REPRODUTIVA FEMININA AO PODER BIOPATRIARCALISTA DE GESTÃO DAS VIDAS HUMANAS: O CONTROLE DOS CORPOS DAS MULHERES MIGRANTES
- Author
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FAPERGS - Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Neilsson, Joice Graciele, Sturza, Janaína Machado, Dezordi Wermuth, Maiquel Ângelo, FAPERGS - Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Neilsson, Joice Graciele, Sturza, Janaína Machado, and Dezordi Wermuth, Maiquel Ângelo
- Abstract
A intercessão entre migração e desigualdades de gênero produz formas distintas de violência e vulnerabilidade, que impactam no acesso a direitos e a políticas de saúde, especialmente sexual e reprodutiva. Este artigo tem por objetivo tratar sobre o tema do direito à saúde reprodutiva das mulheres migrantes, a partir de uma interlocução com o biopatriarcalismo de gestão das vidas humanas e controle dos corpos. Através de um estudo bibliográfico, tendo como método de abordagem o hipotético dedutivo, verificou-se a urgência do desvelamento deste processo de precarização permanente que recai sobre as vidas femininas.
- Published
- 2020
35. A POSSIBILIDADE DE ACESSO JURISDICIONAL DEMOCRÁTICO NO SUPREMO TRIBUNAL FEDERAL: a busca pela efetivação de direitos por meio das audiências públicas e amicus curiae
- Author
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Programa de Aperfeiçoamento de Pessoal de Nível Superior – CAPES, Conselho Nacional de Desenvolvimentos Científico e Tecnológico - CNPq e Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul - FAPERGS, Maia, Selmar José, Rocha, Leonel Severo, Programa de Aperfeiçoamento de Pessoal de Nível Superior – CAPES, Conselho Nacional de Desenvolvimentos Científico e Tecnológico - CNPq e Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul - FAPERGS, Maia, Selmar José, and Rocha, Leonel Severo
- Abstract
O presente estudo sociojurídico objetiva contribuir para uma análise mais sofisticada acerca das demandas sociais ante as decisões jurídicas, visto que não são raras às vezes em que direitos são reivindicados por mobilizações e processos judiciais e, por conseguinte, acabam por encontrar guarida no texto legal e/ou decisões normativas. Por fim, a partir da análise pragmático-sistêmica, adotada neste artigo, observa-se que as irritações comunicacionais introduzidas na sociedade pelas organizações públicas e/ou privadas são importantes para acessos mais democráticos no STF, conforme será possível verificar nos casos estudados nesta pesquisa.
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- 2020
36. Competing interactions near the liquid-liquid phase transition of core-softened water/methanol mixtures
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), CSIC - Instituto de Química Física Rocasolano (IQFR), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Economía y Competitividad (España), Marques, M.S., Hernandes, V.F., Lomba, Enrique, Bordin, J.R., Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), CSIC - Instituto de Química Física Rocasolano (IQFR), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Ministerio de Economía y Competitividad (España), Marques, M.S., Hernandes, V.F., Lomba, Enrique, and Bordin, J.R.
- Abstract
Water is a unique material with a long list of thermodynamic, dynamic and structural anomalies, which are usually attributed to the competition between two characteristic length scales in the intermolecular interaction. It has been argued that a potential liquid-liquid phase transition (LLPT) ending at a liquid-liquid critical point (LLCP) lies at the core of the anomalous behavior of water. This transition which has been evidenced in multiple simulation studies seems to be preempted experimentally by spontaneous crystallization. Here, in order to expose the connection between the spontaneous crystallization observed in the supercooled regime in the vicinity of the LLPT, and the density anomaly, we perform extensive Molecular Dynamics simulations of a model mixture of core-softened water and methanol. The pure water-like fluid exhibits a LLPT and a density anomaly. In contrast, our pure methanol-like model does have a LLPT but lacks the density anomaly. Our results illustrate the relation between the vanishing of the density anomaly and an increase in the temperature of the spontaneous crystallization: once this temperature surpasses the LLCP critical temperature, no density anomaly is observed. This peculiar feature illustrates how fine tuning the competitive interactions determine the anomalous behavior of water/alcohol mixtures.
- Published
- 2020
37. Cadmium exposure activates NADPH oxidase, renin–angiotensin system and cyclooxygenase 2 pathways in arteries, inducing hypertension and vascular damage
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Universidade Federal do Pampa, Fundação de Amparo à Pesquisa do Estado de São Paulo, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Gomes Pinheiro Júnior, José Eudes, Zambelli Moraes, Paola, Diaz Rodriguez, Marina, Ronacher Simões, Maylla, Cibin, Francielli, Pinton, Simone, Barbosa Junior, Fernando, Peçanha, Franck Maciel, Vassallo, Dalton Valentim, Miguel, Marta, Wiggers, Giulia Alessandra, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Universidade Federal do Pampa, Fundação de Amparo à Pesquisa do Estado de São Paulo, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Gomes Pinheiro Júnior, José Eudes, Zambelli Moraes, Paola, Diaz Rodriguez, Marina, Ronacher Simões, Maylla, Cibin, Francielli, Pinton, Simone, Barbosa Junior, Fernando, Peçanha, Franck Maciel, Vassallo, Dalton Valentim, Miguel, Marta, and Wiggers, Giulia Alessandra
- Abstract
Exposure to high concentrations of cadmium (Cd), widely used in many industries and found in air, food and contaminated water, is not uncommon. Cd damages the cardiovascular system, but the vascular mechanisms involved are not fully understood. This study investigated the mechanisms involved in cardiovascular damage after exposure to high Cd concentrations. Three-month-old male Wistar rats were treated intraperitoneally for 14 days with distilled water (Untreated group) or 1 mg/kg cadmium chloride (Cd group). We investigated the systolic blood pressure (SBP) and vascular reactivity of mesenteric resistance arteries (MRA) and the aorta by analysing contractile and relaxation responses in the absence and presence of the endothelium; we also evaluated pathways involved in vascular tone regulation. Superoxide anion production, COX-2 protein expression and in situ detection of COX-2, AT-1, and NOX-1 were evaluated. Oxidative status, creatinine level and angiotensin-converting enzyme (ACE) activity in plasma were also evaluated. Fourteen-day exposure to a high Cd concentration induced hypertension associated with vascular dysfunction in MRA and the aorta. In both vessels, there was increased participation of cyclooxygenase 2 (COX2), angiotensin II type 1 (AT1) receptor and NOX1. MRA also presented endothelial dysfunction, denoted by impaired acetylcholine-mediated relaxation. All vascular changes were accompanied by increased reactive oxygen species production and COX2, NOX1 and AT1 receptor expression in vascular tissue. Overall, high Cd concentrations induced cardiovascular damage: hypertension, endothelial dysfunction and vascular damage in conductance and resistance arteries, NADPH oxidase, renin–angiotensin system and COX2 pathway activation.
- Published
- 2020
38. Lignin degradation and detoxification of eucalyptus wastes by on-site manufacturing fungal enzymes to enhance second-generation ethanol yield
- Author
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Agencia Estatal de Investigación (España), Universidade de Caxias do Sul, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Comunidad de Madrid, Empresa Brasileira de Pesquisa Agropecuária, Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Consejo Superior de Investigaciones Científicas (España), de Siqueira, Félix Gonçalves [0000-0001-5239-4994], Rencoret, Jorge [0000-0003-2728-7331], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Martínez, Ángel T. [0000-0002-1584-2863], Schneider, Willian Daniel Hahn, Fontana, Roselei Claudete, Baudel, Henrique M., de Siqueira, Félix Gonçalves, Rencoret, Jorge, Gutiérrez Suárez, Ana, Eugenio, Laura I. de, Prieto Orzanco, Alicia, Martínez, María Jesús, Martínez, Ángel T., Dillon, Aldo José Pinheiro, Camassola, Marli, Agencia Estatal de Investigación (España), Universidade de Caxias do Sul, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Comunidad de Madrid, Empresa Brasileira de Pesquisa Agropecuária, Ministerio de Ciencia, Innovación y Universidades (España), European Commission, Consejo Superior de Investigaciones Científicas (España), de Siqueira, Félix Gonçalves [0000-0001-5239-4994], Rencoret, Jorge [0000-0003-2728-7331], Gutiérrez Suárez, Ana [0000-0002-8823-9029], Martínez, Ángel T. [0000-0002-1584-2863], Schneider, Willian Daniel Hahn, Fontana, Roselei Claudete, Baudel, Henrique M., de Siqueira, Félix Gonçalves, Rencoret, Jorge, Gutiérrez Suárez, Ana, Eugenio, Laura I. de, Prieto Orzanco, Alicia, Martínez, María Jesús, Martínez, Ángel T., Dillon, Aldo José Pinheiro, and Camassola, Marli
- Abstract
Novel laccases have promising and valuable applications in biorefineries. This investigation documents, for the first time, the potential of depolymerising and repolymerising lignin by the secretome, rich in laccases, from a newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111, for further saccharification and ethanolic fermentation steps. Proteomic analyses of the secretome of M. palmivorus show that laccases are the most predominant enzyme released by this fungus. The whole crude enzymatic broth is used for the delignification of lignin in Eucalyptus globulus wood, with the aim of enhancing the saccharification by cellulolytic and xylanolytic enzymes from Penicillium echinulatum S1M29. In addition, two different strategies, namely, laccase treatment before and after enzymatic hydrolysis, are employed to detoxify steam-exploded E. globulus wood. The objective is to increase the fermentative performance by removing substances formed during the feedstock pretreatment that can inhibit microbial fermentation. The E. globulus wood delignification results in a 31% decrease in the lignin content and a 10% increase in the glucose yield after hydrolysis. An important finding of the present work is the successful wood delignification in the absence of laccase mediators. This laccase-rich preparation also demonstrates its potential in removing the phenolic inhibitors present in steam-exploded E. globulus wood, increasing the ethanol yield by an additional 10%. Furthermore, it is important to highlight that these findings are achieved in the absence of commercial enzymes, making M. palmivorus laccases a potential candidate not only for the production of biofuels but also for the generation of lignin-derived aromatic compounds for different applications in the biotechnology industry. © 2020 Elsevier Ltd
- Published
- 2020
39. DIREITOS HUMANOS E A ESTERILIZAÇÃO DE MULHERES NO BRASIL: O CONTROLE REPRODUTIVO SOBRE OS CORPOS FEMININOS
- Author
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FAPERGS - Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, NIELSSON, JOICE GRACIELE, FAPERGS - Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, and NIELSSON, JOICE GRACIELE
- Abstract
O artigo analisa a evolução das políticas de planejamento familiar e esterilização de mulheres no Brasil, evidenciando a ambivalência entre a promoção dos direitos humanos reprodutivos e as práticas de controle que, pelo dispositivo da reprodutividade, instituem cesuras biopatriarcalistas entre corpos úteis e indesejáveis à reprodução. Analisa a aplicação, por meio de políticas públicas e decisões jurisprudências, da Lei de Planejamento Familiar -9263/96, quanto à prática de esterilização, destacando que, através da exceção, instrumentalizam-se diferentes formas de gestão do útero, conforme recortes de raça, classe, discapacidade e outras. Utiliza o método estruturalista, e o método de abordagem hipotético dedutivo.
- Published
- 2020
40. The judiciary and the greek myth of Kronos: the judicialization of the consensual means for conflict solution and the monopoly of access to justice
- Author
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS)., Stangherlin, Camila Silveira, Spengler, Fabiana Marion, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS)., Stangherlin, Camila Silveira, and Spengler, Fabiana Marion
- Abstract
In the Greek mythology, the god Kronos swallowed his children after birth, in order not to be dethroned and thus to perpetuate himself in power. The Brazilian State, when represented by the Judiciary, in seeking ways to provide access to qualified justice, has been overusing extrajudicial forms – which at community level have provided differentiated approaches to conflict – but such overuse has transformed these extrajudicial decisions into rules of traditional jurisdiction. The present study aims at analyzing the perspective of the Judiciary at implementing alternatives to adjudicated decisions, by means of editing norms that keep it as a centralizing entity, through self-determined means outlined under its order. The research is based on the bibliographical method and contains an examination of the doctrine and legislation connected to the theme, as well as the hypothetical-deductive approach and monographic procedure method. In this understanding, one wonders: from the myth of the Greek god Kronos, is it possible to identify an excess – by the Judiciary – in subtracting consensual ways of resolving conflicts in order to retain the monopoly of jurisdiction? The incessant intervention of the Brazilian Judiciary is incontestable in covering all the questions related to the delineation of the self-composition of conflicts, infecting them with the perils of a decadent system, hence separating the citizen from the access to justice., Na mitologia grega, o deus Cronos engolia seus filhos após o nascimento, no intuito de não ser destronado e, assim, perpetuar-se no poder. O Estado, representado pelo Poder Judiciário, ao almejar vias que possibilitem o acesso à justiça qualificado, vem balizando formas extrajudiciais – que em âmbito comunitário propiciavam uma abordagem diferenciada do conflito – e convertendo-as aos ditames da jurisdição tradicional. O presente estudo objetiva analisar a perspectiva do Poder Judiciário em implementar alternativas à decisão adjudicada, pela edição de normas que o mantém como ente centralizador, através de meios autocompositivos delineados sob seu crivo. A pesquisa vale-se de técnica bibliográfica, contendo exame da doutrina e legislação condizente ao tema, bem como utilizando o método de abordagem hipotético-dedutivo e método de procedimento monográfico. Nessa compreensão, questiona-se: a partir do mito do deus grego Cronos, é possível identificar um excesso – por parte do Poder Judiciário – de subtração das formas consensuais de solucionar conflitos, a fim de manter-se no monopólio da jurisdição? É inconteste a incessante intervenção do Poder Judiciário em abarcar todas as questões relacionadas ao deslinde da autocomposição de conflitos, eivando-as com as mazelas próprias de um sistema em decadência e afastando o cidadão do acesso à justiça.
- Published
- 2019
41. DIÁLOGO ENTRE SPIVAK E HONNETH: A LIBERDADE SOCIAL COMO UM TRAMPOLIM AO SUBALTERNO
- Author
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Fundação de Amparo à pesquisa do Estado do Rio Grande do Sul, Julio, Ana Carolina Cavalcante Ferreira, Fundação de Amparo à pesquisa do Estado do Rio Grande do Sul, and Julio, Ana Carolina Cavalcante Ferreira
- Abstract
O artigo propõe uma costura entre os conceitos de “Liberdade Social”, de Honneth (2015) e “Subalternidade” e “Colonialidade”, de Spivak (2010), entendendo que reconhecem a importância da dinâmica social para a ideia de justiça. Considerando que em decorrência da complexidade na comunicação do sistema jurídico pela linguagem, cria-se uma distância entre a população subalterna e a efetivação dos seus direitos, portanto, uma patologia da liberdade jurídica. Assim, propõe-se uma quebra nessa forma de pensar restrita ao direito, apontando a importância da abertura à realidade social. Utiliza-se, então, a metodologia da revisão bibliográfica e da análise qualitativa de dados estatísticos.
- Published
- 2019
42. Novozym 435: The 'perfect' lipase immobilized biocatalyst?
- Author
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Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Industria, Comercio y Turismo (España), Colciencias (Colombia), Generalitat Valenciana, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Consejo Superior de Investigaciones Científicas (España), Ministerio de Educación Nacional (Colombia), ICETEX (Colombia), Fondo Francisco José de Caldas, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico, Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Dos Santos, José C. S [0000-0002-1511-5180], Berenguer-Murcia, Ánge [0000-0003-3204-801X], Briand, Laura E. [0000-0002-6828-8624], Fernández-Lafuente, Roberto [0000-0003-4976-7096], Ortiz, Claudia, Ferreira, María Luján, Barbosa, Oveimar, Dos Santos, José C. S., Rodrigues, Rafael C., Berenguer-Murcia, Ángel, Briand, Laura E., Fernández-Lafuente, Roberto, Ministerio de Ciencia, Innovación y Universidades (España), Ministerio de Industria, Comercio y Turismo (España), Colciencias (Colombia), Generalitat Valenciana, Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Consejo Superior de Investigaciones Científicas (España), Ministerio de Educación Nacional (Colombia), ICETEX (Colombia), Fondo Francisco José de Caldas, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico, Agencia Nacional de Promoción Científica y Tecnológica (Argentina), Dos Santos, José C. S [0000-0002-1511-5180], Berenguer-Murcia, Ánge [0000-0003-3204-801X], Briand, Laura E. [0000-0002-6828-8624], Fernández-Lafuente, Roberto [0000-0003-4976-7096], Ortiz, Claudia, Ferreira, María Luján, Barbosa, Oveimar, Dos Santos, José C. S., Rodrigues, Rafael C., Berenguer-Murcia, Ángel, Briand, Laura E., and Fernández-Lafuente, Roberto
- Abstract
Novozym 435 (N435) is a commercially available immobilized lipase produced by Novozymes. It is based on immobilization via interfacial activation of lipase B from Candida antarctica on a resin, Lewatit VP OC 1600. This resin is a macroporous support formed by poly(methyl methacrylate) crosslinked with divinylbenzene. N435 is perhaps the most widely used commercial biocatalyst in both academy and industry. Here, we review some of the success stories of N435 (in chemistry, energy and lipid manipulation), but we focus on some of the problems that the use of this biocatalyst may generate. Some of these problems are just based on the mechanism of immobilization (interfacial activation) that may facilitate enzyme desorption under certain conditions. Other problems are specific to the support: Mechanical fragility, moderate hydrophilicity that permits the accumulation of hydrophilic compounds (e.g., water or glycerin) and the most critical one, support dissolution in some organic media. Finally, some solutions (N435 coating with silicone, enzyme physical or chemical crosslinking, and use of alternative supports) are proposed. However, the N435 history, even with these problems, may continue in the coming future due to its very good properties if some simpler alternative biocatalysts are not developed.
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- 2019
43. Development of new treatments for Alzheimer's disease based on the modulation of translocator protein (TSPO)
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Arbo, B. D., Ribeiro, M. F. B., García-Segura, Luis M., Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Arbo, B. D., Ribeiro, M. F. B., and García-Segura, Luis M.
- Abstract
The increase in life expectancy of the world population is associated with a higher prevalence of neurodegenerative diseases. Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting currently 43 million people over the world. To date, most of the pharmacological interventions in AD are intended for the alleviation of some of its symptoms, and there are no effective treatments to inhibit the progression of the disease. Translocator protein (TSPO) is present in contact points between the outer and the inner mitochondrial membranes and is involved in the control of steroidogenesis, inflammation and apoptosis. In the last decade, studies have shown that TSPO ligands present neuroprotective effects in different experimental models of AD, both in vitro and in vivo. The aim of this review is to analyze the data provided by these studies and to discuss if TSPO could be a viable therapeutic target for the development of new treatments for AD.
- Published
- 2019
44. A sinfoni view of the nuclear activity and circumnuclear star formation in NGC 4303 – II. Spatially resolved stellar populations
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Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Economía y Competitividad (España), Fundação de Amparo à Pesquisa do Estado de São Paulo, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Dametto, N.Z., Riffel, Rogério, Colina, Luis, Riffel, Rogemar A., Piqueras López, Javier, Davies, R. I., Burtscher, Leonard, Menezes, R. B., Arribas-Mocoroa, Santiago, Pastoriza, M., Labiano, Álvaro, Storchi Bergmann, T., Dahmer-Hahn, L.G., Sales, Dinalva A., Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Ministerio de Economía y Competitividad (España), Fundação de Amparo à Pesquisa do Estado de São Paulo, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Dametto, N.Z., Riffel, Rogério, Colina, Luis, Riffel, Rogemar A., Piqueras López, Javier, Davies, R. I., Burtscher, Leonard, Menezes, R. B., Arribas-Mocoroa, Santiago, Pastoriza, M., Labiano, Álvaro, Storchi Bergmann, T., Dahmer-Hahn, L.G., and Sales, Dinalva A.
- Abstract
We present a spatially resolved stellar population study of the inner ∼200 pc radius of NGC 4303 based on near-infrared integral field spectroscopy with SINFONI/VLT at a spatial resolution of 40–80 pc and using the STARLIGHT code. We found that the distribution of the stellar populations presents a spatial variation, suggesting an age stratification. Three main structures stand out. Two nuclear blobs, one composed by young stars (t ≤ 50 Myr) and one with intermediate-age stars (50 Myr < t ≤ 2 Gyr), both shifted from the centre. The third one is an internal intermediate-age spiral arm-like structure, surrounding the blob of young stars. Our results indicate that star formation has occurred through multiple bursts in this source. Furthermore, the youngest stellar populations (t 2 Gyr) are distributed along a circumnuclear star-forming ring with r ∼ 250 pc. The ring displays star formation rates (SFRs) in the range of 0.002–0.14 Myr, favouring the ‘pearls-on-a-string’ scenario. The old underlying bulge stellar population component (t > 2 Gyr) is distributed outside the two blob structures. For the nuclear region (inner ∼60 pc radius) we derived an SFR of 0.43 M yr and found no signatures of non-thermal featureless continuum and hot dust emission, supporting the scenario in which an LLAGN/LINER-like source is hidden in the centre of NGC 4303. Thus, our results reveal a rather complex star formation history in NGC 4303, with different stellar population components coexisting with a low efficiency accreting black hole in its centre.
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- 2019
45. Chlorophyll Oxidative Metabolism During the Phototrophic and Heterotrophic Growth of Scenedesmus obliquus
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Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Manzoni Maroneze, Mariana, Queiroz Zepka, Leila, Jacob Lopes, Eduardo, Pérez Gálvez, Antonio, Roca, María, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Consejo Superior de Investigaciones Científicas (España), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Manzoni Maroneze, Mariana, Queiroz Zepka, Leila, Jacob Lopes, Eduardo, Pérez Gálvez, Antonio, and Roca, María
- Abstract
Different cultivation strategies have been developed with the aim of increasing the production rate of microalgal pigments. Specifically, biotechnological approaches are designed to increase antioxidant metabolites as chlorophyll and carotenoids. However, although significant advances have been built up, available information regarding both the chlorophyll metabolism and their oxidative reactions in photobioreactors is scarce. To unravel such processes, the detailed chlorophyll and carotenoid fraction of Scenedesmus obliquus has been studied by HPLC-ESI/APCI-hrTOF-MS from phototrophic and heterotrophic cultures. Scenedesmus is provided with a controlled strategy of interconversion between chlorophyll a and b to avoid the formation of reactive oxygen species (ROS) at high irradiances in addition to the photoacclimation of carotenoids. Indeed, precise kinetics of 132-hydroxy- and 151-hydroxy-lactone chlorophyll metabolites shows the existence of a chlorophyll oxidative metabolism as a tool to manage the excess of energy at high light conditions. Unexpectedly, the oxidation under phototrophy favored chlorophyll b metabolites over the chlorophyll a series, while the heterotrophic conditions exclusively induced the formation of 132-hydroxy-chlorophyll a. In parallel, during the first 48 h of growth in the dark, the chlorophyll fraction maintained a promising steady state. Although future studies are required to resolve the biochemical reactions implied in the chlorophyll oxidative metabolism, the present results agree with phytoplankton metabolism.
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- 2019
46. Pluto's ephemeris from ground-based stellar occultations (1988-2016)
- Author
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), European Research Council, Desmars, J., Meza, E., Sicardy, B., Assafin, M., Camargo, J.I.B., Braga-Ribas, F., Benedetti-Rossi, G., Dias-Oliveira, A., Morgado, B., Gomes-Júnior, A.R., Vieira-Martins, Roberto, Behrend, R., Ortiz, José Luis, Duffard, René D., Morales, Nicolás, Santos Sanz, Pablo, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Brasil), European Research Council, Desmars, J., Meza, E., Sicardy, B., Assafin, M., Camargo, J.I.B., Braga-Ribas, F., Benedetti-Rossi, G., Dias-Oliveira, A., Morgado, B., Gomes-Júnior, A.R., Vieira-Martins, Roberto, Behrend, R., Ortiz, José Luis, Duffard, René D., Morales, Nicolás, and Santos Sanz, Pablo
- Abstract
Context. From 1988 to 2016, several stellar occultations have been observed to characterise Pluto's atmosphere and its evolution. From each stellar occultation, an accurate astrometric position of Pluto at the observation epoch is derived. These positions mainly depend on the position of the occulted star and the precision of the timing. Aims. We present 19 Pluto's astrometric positions derived from occultations from 1988 to 2016. Using Gaia DR2 for the positions of the occulted stars, the accuracy of these positions is estimated at 2-10 mas, depending on the observation circumstances. From these astrometric positions, we derive an updated ephemeris of Pluto's system barycentre using the NIMA code. Methods. The astrometric positions were derived by fitting the light curves of the occultation by a model of Pluto's atmosphere. The fits provide the observed position of the centre for a reference star position. In most cases other publications provided the circumstances of the occultation such as the coordinates of the stations, timing, and impact parameter, i.e. the closest distance between the station and centre of the shadow. From these parameters, we used a procedure based on the Bessel method to derive an astrometric position. Results. We derive accurate Pluto's astrometric positions from 1988 to 2016. These positions are used to refine the orbit of Pluto'system barycentre providing an ephemeris, accurate to the milliarcsecond level, over the period 2000-2020, allowing for better predictions for future stellar occultations.© J. Desmars et al. 2019.
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- 2019
47. ENTRE A ARTE E O ENSINO DO DIREITO: NOTAS SOBRE NOSSAS LINHAS DE FUGA
- Author
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Fundação de Amparo à pesquisa do Estado do Rio Grande do Sul, Julio, Ana Carolina Cavalcante Ferreira, Henning, Ana Clara Correa, Fundação de Amparo à pesquisa do Estado do Rio Grande do Sul, Julio, Ana Carolina Cavalcante Ferreira, and Henning, Ana Clara Correa
- Abstract
Partindo da conexão entre direito e arte, debatemos saberes sujeitados ou subalterizados e suas possíveis resistências ao problematizar, no âmbito do ensino jurídico, normas de direito e sua eficácia social. Apresentamos, para isso, os resultados de um projeto de ensino, pesquisa e extensão, realizado desde o ano de 2017 em uma Faculdade de Direito do sul do Brasil, por meio de pesquisa documental e de fundamentação em literatura especializada no campo do direito e arte, em estudos pós-estruturalistas e decoloniais. Com isso, buscamos criar algumas linhas de fuga, na busca pela democratização do conhecimento jurídico.
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- 2019
48. Electrodialysis for the tertiary treatment of municipal wastewater: Efficiency of ion removal and ageing of ion exchange membranes
- Author
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Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear, Universidade Feevale, Brasil, Câmara Municipal de Novo Hamburgo, Brasil, Financiadora de Estudos e Projetos, Brasil, Banco Nacional de Desenvolvimento Econômico e Social, Brasil, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, Rodrigues Gally, Caline, Benvenuti, Tatiane, da Trinidade, Carolina de Moraes, Siqueira Rodrigues, Marco Antonio, Zoppas Ferreira, Jane, Pérez-Herranz, Valentín, Moura Bernardes, Andréa, Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear, Universidade Feevale, Brasil, Câmara Municipal de Novo Hamburgo, Brasil, Financiadora de Estudos e Projetos, Brasil, Banco Nacional de Desenvolvimento Econômico e Social, Brasil, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, Rodrigues Gally, Caline, Benvenuti, Tatiane, da Trinidade, Carolina de Moraes, Siqueira Rodrigues, Marco Antonio, Zoppas Ferreira, Jane, Pérez-Herranz, Valentín, and Moura Bernardes, Andréa
- Abstract
[EN] Electrodialysis was applied as a tertiary treatment for effluents from a Brazilian sewage treatment plant, and the results are discussed in terms of membrane ageing and process efficiency. Current-voltage analysis and electrodialysis (ED) treatment were performed in a bench cell. The treatment was discontinuously carried out for 930 h within one year. During the experiments, samples were collected for evaluation, and the pH, conductivity and ion concentration were monitored. Thermogravimetric analyses of the membrane were also performed. A reduction in electrical conductivity and the high ion percentage extraction demonstrated the efficiency of the ED treatment, confirming the possibility of using ED as a tertiary treatment for sewage. ED showed 100% effectiveness in terms of meeting the quality standards established by Brazilian legislation on the discharge of effluents. Additionally, important corrosive (Cl-) and encrusting ions (Ca-2 +/- and Mg-2 +/- ) that limit certain industrial uses of water were satisfactorily removed, giving the treated effluent a suitable quality for industrial purposes. The treatment did not suffer harmful fouling effects in terms of ion extraction in the membrane ageing study, indicating the possibility of long-term treatment without requiring cleaning for the membranes; however, this needs to be validated by scaling up the process.
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- 2018
49. Antibiotics mineralization by electrochemical and UV-based hybrid processes: evaluation of the synergistic effect
- Author
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Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear, Financiadora de Estudos e Projetos, Brasil, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, CYTED Ciencia y Tecnología para el Desarrollo, Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, da Silva, Salatiel W., Heberle, Alan N.A., Santos, Alexia P., Rodrigues, M.A.S., Valentín Pérez-Herranz, Bernardes, A.M., Universitat Politècnica de València. Departamento de Ingeniería Química y Nuclear - Departament d'Enginyeria Química i Nuclear, Financiadora de Estudos e Projetos, Brasil, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, CYTED Ciencia y Tecnología para el Desarrollo, Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior, Brasil, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brasil, da Silva, Salatiel W., Heberle, Alan N.A., Santos, Alexia P., Rodrigues, M.A.S., Valentín Pérez-Herranz, and Bernardes, A.M.
- Abstract
[EN] Antibiotics are not efficiently removed in conventional wastewater treatments. In fact, different advanced oxidation process (AOPs), including ozone, peroxide, UV radiation, among others, are being investigated in the elimination of microcontaminants. Most of AOPs proved to be efficient on the degradation of antibiotics, but the mineralization is on the one hand not evaluated or on the other hand not high. At this work, the UV-based hybrid process, namely Photo-assisted electrochemical oxidation (PEO), was applied, aiming the mineralization of microcontaminants such as the antibiotics Amoxicillin (AMX), Norfloxacin (NOR) and Azithromycin (AZI). The influence of the individual contributions of electrochemical oxidation (EO) and the UV-base processes on the hybrid process (PEO) was analysed. Results showed that AMX and NOR presented higher mineralization rate under direct photolysis than AZI due to the high absorption of UV radiation. For the EO processes, a low mineralization was found for all antibiotics, what was associated to a mass-transport limitation related to the low concentration of contaminants (200 ¿g/L). Besides that, an increase in mineralization was found, when heterogeneous photocatalysis and EO are compared, due to the influence of UV radiation, which overcomes the mass-transport limitations. Although the UV-based processes control the reaction pathway that leads to mineralization, the best results to mineralize the antibiotics were achieved by PEO hybrid process. This can be explained by the synergistic effect of the processes that constitute them. A higher mineralization was achieved, which is an important and useful finding to avoid the discharge of microcontaminants in the environment.
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- 2018
50. Effects of agar films incorporated with fish protein hydrolysate or clove essential oil on flounder (Paralichthys orbignyanus) fillets shelf-life
- Author
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Ministerio de Economía y Competitividad (España), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Da Rocha, Meritaine, Alemán, Ailén, Romani, Viviane Patrícia, López Caballero, M. E., Gómez Guillén, M. C., Montero García, Pilar, Prentice, Carlos, Ministerio de Economía y Competitividad (España), Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul, Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil), Da Rocha, Meritaine, Alemán, Ailén, Romani, Viviane Patrícia, López Caballero, M. E., Gómez Guillén, M. C., Montero García, Pilar, and Prentice, Carlos
- Abstract
This study evaluated the effect of protein hydrolysate (PH) and clove essential oil (CEO) on agar film properties and the shelf-life improvement of flounder fillets. Firstly, Argentine croaker muscle protein was alkali-solubilised and recovered to obtain a stable substrate. This substrate was hydrolysed to 20% degree of hydrolysis, by Alcalase (A20) and Protamex (P20). The molecular weight (MW) distribution, amino acid and in vitro antimicrobial activity of these hydrolysates were tested. Agar films were prepared with and without (control) incorporation of PH (A20 hydrolysate) or CEO and characterised by mechanical properties, water vapour permeability, solubility and optical properties. The microbiological, physical and chemical properties of flounder fillets coated with these films were monitored during storage at 5 °C for 15 days. Compared to P20, A20 hydrolysate possessed lower MW, higher hydrophobic amino acid content and the most effective antimicrobial activity against selected pathogens, such as Staphylococcus aureus, Yersinia enterocolitica, Aeromonas hydrophila, Debaryomyces hansenii and Listeria innocua. The presence of PH increased the water solubility, water vapour permeability, elongation at break and yellowness of the films. The CEO films possessed less transparency than PH films and control films, due to the presence of oil in the formulations. Fillets covered with PH and CEO films, especially the latter, presented lower total volatile bases and pH values, and delayed considerably the growth of HS-producing microorganisms compared to the control films. The CEO might be used as a natural biopreservative to extend the flounder shelf-life. However, further studies are necessary, to assess the antimicrobial effect of PH when used as food packaging.
- Published
- 2018
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