66 results on '"Fernandes DR"'
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2. Mysterious Mass with a Coat of Fur-Fur
- Author
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Vaz, Veril, primary, Bhat M, DR Ramesha, additional, Fernandes, DR Michelle, additional, Dsouza, DR Myfanwy, additional, and Fernandes, DR Sonal, additional
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- 2024
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3. RESILIÊNCIA DOS PROFISSIONAIS DE SAÚDE NO ENFRENTAMENTO DO NOVO CORONAVÍRUS
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Maria Berenice Gomes Nascimento, Marcelo Costa Fernandes, Dr, Silvia Carla Conceição Massagli, Dra, Rafaelle Cavalcante de Lira, Dra, and Fabiana Ferraz Queiroga Freitas, Dra
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Infecções por coronavirus. Pessoal de saúde. Resiliência psicológica. Saúde mental. Impactos na saúde. ,Nursing ,RT1-120 - Abstract
Objetivo: Discutir a resiliência de profissionais de saúde no enfrentamento do novo coronavírus, a partir de um estudo teórico-reflexivo. Metodologia: Refletir a partir de um olhar do construto teórico da resiliência delineado pela autora Yunes as situações adversas experienciadas pelos profissionais da saúde que estão cuidando de pacientes com COVID-19. Os depoimentos foram extraídos de telejornais entre os meses de março e abril de 2020. Resultados: A pandemia do coronavírus trouxe graves consequências para os profissionais da atenção à saúde, assim despertando a necessidade de minimizar impactos na saúde mental destes. Apreende-se que os fatores de riscos evidenciados externam o medo de contrair a doença, falta de equipamentos de proteção individual, bem como o medo de morrer e deixar os filhos ou ainda serem agentes transmissores da doença. Conclusão: A condição observada gera ansiedade, angústia, revelando a necessidade e importância dos profissionais da saúde também serem cuidados, para que ao final dessa jornada estejam com a saúde mental preservada.
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- 2022
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4. O processo de ação coletiva na produção de bens comuns
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Alex Bruno Ferreira Marques do Nascimento, Dr., Antonio Sérgio Aráujo Fernandes, Dr., Mauro Guilherme Maidana Capelari, Dr., Marco Antonio Carvalho Teixeira, Dr., and Eduardo José Grin, Dr,
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Ação Coletiva. Institutional Collective Action. Institutional Analysis Development ,Political science - Abstract
O papel das instituições na produção de bens comuns em processos de ação coletiva é chave para explicar como as instituições em seu contexto influenciam os atores a agir. Para isso, o artigo traz dois quadros teóricos que têm se destacados na literatura recente como arcabouços para a compreensão do processo de ação coletiva visando a produção de bens comuns: o Institutional Analysis Development (IAD), e o Institutional Collective Action (ICA). Sendo assim, o objetivo desse ensaio teórico é caracterizar cada um desses frameworks versando sobre seus pontos de similaridades e diferenças. Ao fim, percebeu-se que ambas partem do princípio de que a ação coletiva é influenciada por instituições contextuais locais, que influenciam a cooperação em diferentes formatos e momentos. .
- Published
- 2021
5. Ações educativas como possibilidade no repensar do homem cigano frente ao consumo do cigarro
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Isabela Lunara Alves Barbalho, Geísa Batista Leandro, Raquel de Jesus Rocha da Silva, Mariana Alexandre Gadelha de Lima, and Marcelo Costa Fernandes, Dr
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Tabagismo ,Roma (Grupo Étnico) ,Homens ,Saúde ,Atenção Primária à Sáude ,Nursing ,RT1-120 - Abstract
O estudo objetiva oportunizar ambientes de discussões sobre as consequências oriundas do consumo do tabaco ao indivíduo e a coletividade, propiciando a melhoria da qualidade de vida desse segmento da população. Trata-se de estudo descritivo com abordagem qualitativa com utilização da pesquisa-ação. O mesmo foi desenvolvido com ciganos tabagistas, da comunidade cigana, Rancho de Cima, da cidade de Sousa, no estado da Paraíba. A pesquisa teve início, a partir da aprovação do projeto pelo Comitê de Ética em Pesquisa sob o parecer nº 2.338.568. As atividades proporcionaram a discussão dos prejuízos decorrente do tabagismo, expondo mecanismos de contribuição e estratégias de formas de repensar no agir dos homens ciganos tabagistas frente ao vício, com foco em ações de educação em saúde, de forma a compartilhar saberes, vivências e experiências. A promoção do seguinte estudo propiciou a análise e discussão das experiências do homem cigano acerca do consumo do cigarro. Além de oportunizar o compartilhamento de conhecimentos adquiridos, das experiências vividas e atribuir a realização de ações educativas para fomentar o repensar do homem cigano em relação ao consumo do tabaco.
- Published
- 2021
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6. The Brazilian Government's Inaction Policy in the face of the COVID-19 pandemic
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Marco Teixeira, Dr., Antonio Sérgio Aráujo Fernandes, Dr., Robson Zuccolotto, and Alex Bruno F. M. do Nascimento
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Inaction Policy ,Federative Uncoordination ,Insufficient Financial Resources ,Political Crisis ,Brasil ,COVID-19 ,Political science - Abstract
This article aim show the inaction in public policy to combat COVID-19 in Brazil, making the country the second in the number of cases and second in the number of deaths in the world. This situation is due to a political choice from Federal Government that marked the decision-making process of public policies to combat COVID-19. The problem is situated in Federal Governament, and this statement is supported by the conceptual hypothesis of Inaction Policy that will be demonstrated by the analytical narrative throughout the text
- Published
- 2020
7. The use of Machine Learning for predicting the sentence given by the population at a crime scene
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de Freitas, Keyvilânia V. V., Fernandes, Dr. C. Alexandre R., Ferreira M. Jr., Dr. James, and dos Santos, Gabriela A.
- Published
- 2023
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8. A Survey on Underwater Image Processing Techniques
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Ravindran, Arya, primary, Lokapure, Anand, additional, and Fernandes, Dr. Aisha, additional
- Published
- 2022
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9. A case series of gestational trophoblastic disease, it's variants, management and outcomes in a tertiary health care centre: A report of three cases
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Fernandes, Dr. Lynette, primary, Jindal, Dr. Manjusha, additional, and Padwalkar, Dr. Rupa, additional
- Published
- 2022
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10. Efficacy of mitomycin C in adjunct with intralesional steroids in the management of oral submucous fibrosis: A case report
- Author
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Fernandes, Dr. Jerusha, primary, Rana, Dr. Vishal, additional, and Gupta, Dr. Savina, additional
- Published
- 2022
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11. Peri-Implantitis Matter: Possibilities of Treatment but Without a Strong Predictability for Solution
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Vicentis de Oliveira Fernandes, Dr. Gustavo, primary
- Published
- 2021
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12. Getting Started with Python for the Internet of Things
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Fernandes, Dr. Steven Lawrence, Fernandes, Dr. Steven Lawrence, Yamanoor, Sai, Cox, Tim, Fernandes, Dr. Steven Lawrence, Fernandes, Dr. Steven Lawrence, Yamanoor, Sai, and Cox, Tim
- Abstract
Build clever, collaborative, and powerful automation systems with the Raspberry Pi and Python. Key Features Create your own Pi-Rover or Pi-Hexipod robots Develop practical applications in Python using Raspberry Pi Build your own Jarvis, a highly advanced computerized AI Book Description This Learning Path takes you on a journey in the world of robotics and teaches you all that you can achieve with Raspberry Pi and Python. It teaches you to harness the power of Python with the Raspberry Pi 3 and the Raspberry Pi zero to build superlative automation systems that can transform your business. You will learn to create text classifiers, predict sentiment in words, and develop applications with the Tkinter library. Things will get more interesting when you build a human face detection and recognition system and a home automation system in Python, where different appliances are controlled using the Raspberry Pi. With such diverse robotics projects, you'll grasp the basics of robotics and its functions, and understand the integration of robotics with the IoT environment. By the end of this Learning Path, you will have covered everything from configuring a robotic controller, to creating a self-driven robotic vehicle using Python. Raspberry Pi 3 Cookbook for Python Programmers - Third Edition by Tim Cox, Dr. Steven Lawrence Fernandes Python Programming with Raspberry Pi by Sai Yamanoor, Srihari Yamanoor Python Robotics Projects by Prof. Diwakar Vaish What you will learn Build text classifiers and predict sentiment in words with the Tkinter library Develop human face detection and recognition systems Create a neural network module for optical character recognition Build a mobile robot using the Raspberry Pi as a controller Understand how to interface sensors, actuators, and LED displays work Apply machine learning techniques to your models Interface your robots with Bluetooth Who this book is for This Learning Path is specially designed for Python developers who want to take t
- Published
- 2019
13. A CFD Analysis on the Influence of Upstream Surface Geometry Modifications of Clerestory Shaped Rib on Heat Transfer Characteristics of Solar Air Heater
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Fernandes, Dr. Dolfred Vijay, primary and M.S., Manjunath, additional
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- 2021
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14. Maternal and perinatal outcome of eclampsia at a tertiary hospital: A retrospective analysis
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Fernandes, Dr. Jessica Celina, primary and G, Dr. Nandini, additional
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- 2021
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15. Evaluating Effect of Anxiety on Eye Hand Coordination Using Jenga Game in Female Nursing Professionals: An Observational Study
- Author
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Chitra, Dr. Jeba, primary, Fernandes, Dr. Jorida, additional, and Parikh, Jowena, additional
- Published
- 2020
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16. Evaluation of satisfaction and change in knowledge following use of high fidelity simulation based teaching for obstetric emergencies among nursing students
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Fernandes, Dr. Shannon, primary, D'Cunha, Dr Rithesh, additional, and Sherif, Dr. Lulu, additional
- Published
- 2020
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17. Clinico-Histopathological correlation in Hansen’s disease: A retrospective study
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Archana, Dr., primary and Fernandes, Dr. Hilda, additional
- Published
- 2020
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18. Clinical Implications and Histopathological Correlation of Cervical Lesions Found in Cervico-Vaginal Smears
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BR, Dr. Pallavi, primary and Fernandes, Dr. Hilda, additional
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- 2020
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19. Evaluation of therapeutic effect of low-level helium neon laser therapy in the management of concurrent chemoradiotherapy induced mucositis in Oral and ORO-pharyngeal cancer patients
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Nigudgi, Dr. Shantaling, primary and Fernandes, Dr. Donald, additional
- Published
- 2020
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20. Getting Started with Python for the Internet of Things
- Author
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Cox, Tim, Fernandes, Dr. Steven Lawrence, Yamanoor, Sai, Cox, Tim, Cox, Tim, Fernandes, Dr. Steven Lawrence, Yamanoor, Sai, and Cox, Tim
- Abstract
Build clever, collaborative, and powerful automation systems with the Raspberry Pi and Python. Key Features Create your own Pi-Rover or Pi-Hexipod robots Develop practical applications in Python using Raspberry Pi Build your own Jarvis, a highly advanced computerized AI Book Description This Learning Path takes you on a journey in the world of robotics and teaches you all that you can achieve with Raspberry Pi and Python. It teaches you to harness the power of Python with the Raspberry Pi 3 and the Raspberry Pi zero to build superlative automation systems that can transform your business. You will learn to create text classifiers, predict sentiment in words, and develop applications with the Tkinter library. Things will get more interesting when you build a human face detection and recognition system and a home automation system in Python, where different appliances are controlled using the Raspberry Pi. With such diverse robotics projects, you'll grasp the basics of robotics and its functions, and understand the integration of robotics with the IoT environment. By the end of this Learning Path, you will have covered everything from configuring a robotic controller, to creating a self-driven robotic vehicle using Python. Raspberry Pi 3 Cookbook for Python Programmers - Third Edition by Tim Cox, Dr. Steven Lawrence Fernandes Python Programming with Raspberry Pi by Sai Yamanoor, Srihari Yamanoor Python Robotics Projects by Prof. Diwakar Vaish What you will learn Build text classifiers and predict sentiment in words with the Tkinter library Develop human face detection and recognition systems Create a neural network module for optical character recognition Build a mobile robot using the Raspberry Pi as a controller Understand how to interface sensors, actuators, and LED displays work Apply machine learning techniques to your models Interface your robots with Bluetooth Who this book is for This Learning Path is specially designed for Python developers who want to take t
- Published
- 2019
21. Raspberry Pi 3 Cookbook for Python Programmers
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Cox, Tim, Fernandes, Dr. Steven Lawrence, Cox, Tim, Cox, Tim, Fernandes, Dr. Steven Lawrence, and Cox, Tim
- Abstract
A recipe-based guide to programming your Raspberry Pi 3 using PythonAbout This BookLeverage the power of Raspberry Pi 3 using Python programmingCreate 3D games, build neural network modules, and interface with your own circuitsPacked with clear, step-by-step recipes to walk you through the capabilities of Raspberry PiWho This Book Is ForThis book is for anyone who wants to master the skills of Python programming using Raspberry Pi 3. Prior knowledge of Python will be an added advantage.What You Will LearnLearn to set up and run Raspberry Pi 3Build text classifiers and perform automation using PythonPredict sentiments in words and create games and graphicsDetect edges and contours in imagesBuild human face detection and recognition systemUse Python to drive hardwareSense and display real-world dataBuild a neural network module for optical character recognitionBuild movie recommendations systemIn DetailRaspberry Pi 3 Cookbook for Python Programmers – Third Edition begins by guiding you through setting up Raspberry Pi 3, performing tasks using Python 3.6, and introducing the first steps to interface with electronics. As you work through each chapter, you will build your skills and apply them as you progress. You will learn how to build text classifiers, predict sentiments in words, develop applications using the popular Tkinter library, and create games by controlling graphics on your screen. You will harness the power of a built in graphics processor using Pi3D to generate your own high-quality 3D graphics and environments.You will understand how to connect Raspberry Pi's hardware pins directly to control electronics, from switching on LEDs and responding to push buttons to driving motors and servos. Get to grips with monitoring sensors to gather real-life data, using it to control other devices, and viewing the results over the internet. You will apply what you have learned by creating your own Pi-Rover or Pi-Hexipod robots. You will also learn about sentiment analys
- Published
- 2018
22. Observation of an Orbital Interaction-Induced Feshbach Resonance in Yb-173
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Hofer, M, Riegger, L, Scazza, F, Hofrichter, C, Fernandes, Dr, Parish, Mm, Levinsen, J, Bloch, I, Folling, S, Hofer, M, Riegger, L, Scazza, F, Hofrichter, C, Fernandes, Dr, Parish, Mm, Levinsen, J, Bloch, I, and Folling, S
- Abstract
We report on the experimental observation of a novel interorbital Feshbach resonance in ultracold Yb-173 atoms. This opens up the possibility of tuning the interactions between the S-1(0) and P-3(0) metastable state, both possessing zero total electronic angular momentum. The resonance is observed at experimentally accessible magnetic field strengths and occurs universally for all hyperfine state combinations. We characterize the resonance in the bulk via interorbital cross thermalization as well as in a three-dimensional lattice using high-resolution clock-line spectroscopy. Our measurements are well described by a generalized two-channel model of the orbital-exchange interactions.
- Published
- 2015
23. Study of outcome of meconium stained amniotic fluid in newborn
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Fernandes, Dr. Oniell, primary
- Published
- 2018
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24. A study of splenectomy in beta-thalassemia patients and its effects
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Fernandes, Dr. Oniell, primary
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- 2018
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25. From Three Fishers: Statistician, Geneticist and Person to Only One Fisher: The Scientist
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Fernandes DR, Millor, primary
- Published
- 2015
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26. Corn replacement by broken rice in meat-type quail diets
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Filgueira, TMB, primary, Freitas, ER, additional, Quevedo Filho, IB, additional, Fernandes, DR, additional, Watanabe, PH, additional, and Oliveira, AN de, additional
- Published
- 2014
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27. Echocardiographic Markers of Atrial Fibrillation Recurrence Post Radiofrequency Ablation (Echo-If-AF)
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Diogo de Almeida Fernandes, Dr.
- Published
- 2023
28. Direct probing of the Mott crossover in the SU($N$) Fermi-Hubbard model
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Christian Hofrichter, Luis Riegger, Francesco Scazza, Moritz Höfer, Diogo Rio Fernandes, Immanuel Bloch, Simon Fölling, Hofrichter, C, Riegger, L, Scazza, F, Hofer, M, Fernandes, Dr, Bloch, I, and Folling, S
- Subjects
Condensed Matter::Quantum Gases ,Quantum Gases (cond-mat.quant-gas) ,Physics ,QC1-999 ,FOS: Physical sciences ,Condensed Matter::Strongly Correlated Electrons ,Condensed Matter - Quantum Gases - Abstract
We report on a detailed experimental investigation of the equation of state (EoS) of the three-dimensional Fermi-Hubbard model (FHM) in its generalized SU(N)-symmetric form, using a degenerate ytterbium gas in an optical lattice. In its more common spin-1/2 form, the FHM is a central model of condensed-matter physics. The generalization to N > 2 was first used to describe multi-orbital materials and is expected to exhibit novel many-body phases in a complex phase diagram. By realizing and locally probing the SU(N) FHM with ultracold atoms, we obtain model-free access to thermodynamic quantities. The measurement of the EoS and the local compressibility allows us to characterize the crossover from a compressible metal to an incompressible Mott insulator. We reach specific entropies above Neel order but below that of uncorrelated spins. Having access to the EoS of such a system represents an important step towards probing predicted novel SU(N) phases.
- Published
- 2015
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29. Laminin-α2 chain deficiency in skeletal muscle causes dysregulation of multiple cellular mechanisms.
- Author
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Martins SG, Ribeiro V, Melo C, Paulino-Cavaco C, Antonini D, Dayalan Naidu S, Murtinheira F, Fonseca I, Saget B, Pita M, Fernandes DR, Gameiro Dos Santos P, Rodrigues G, Zilhão R, Herrera F, Dinkova-Kostova AT, Carlos AR, and Thorsteinsdóttir S
- Subjects
- Animals, Mice, DNA Damage, Muscular Dystrophies metabolism, Muscular Dystrophies genetics, Muscular Dystrophies pathology, Cell Proliferation genetics, Cell Line, Muscle Development genetics, Disease Models, Animal, Mutation, Muscle Fibers, Skeletal metabolism, Laminin metabolism, Laminin genetics, Laminin deficiency, Muscle, Skeletal metabolism, Muscle, Skeletal pathology, Cell Differentiation genetics, Myoblasts metabolism, Oxidative Stress genetics
- Abstract
LAMA2, coding for the laminin-α2 chain, is a crucial ECM component, particularly abundant in skeletal muscle. Mutations in LAMA2 trigger the often-lethal LAMA2 -congenital muscular dystrophy (LAMA2-CMD). Various phenotypes have been linked to LAMA2-CMD; nevertheless, the precise mechanisms that malfunction during disease onset in utero remain unknown. We generated Lama2 -deficient C2C12 cells and found that Lama2 -deficient myoblasts display proliferation, differentiation, and fusion defects, DNA damage, oxidative stress, and mitochondrial dysfunction. Moreover, fetal myoblasts isolated from the dy
W mouse model of LAMA2-CMD display impaired differentiation and fusion in vitro. We also showed that disease onset during fetal development is characterized by a significant down-regulation of gene expression in muscle fibers, causing pronounced effects on cytoskeletal organization, muscle differentiation, and altered DNA repair and oxidative stress responses. Together, our findings provide unique insights into the critical importance of the laminin-α2 chain for muscle differentiation and muscle cell homeostasis., (© 2024 Martins et al.)- Published
- 2024
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30. Zeolites as Solid Solvents: Explaining the Chloromethane Hydrolysis over Metal-Exchanged Zeolite Y by DFT Calculations.
- Author
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Rosenbach N Jr, Fernandes DR, and Mota CJA
- Abstract
We report a theoretical DFT study of the reaction pathways for chloromethane hydrolysis over metal-exchanged zeolites (Li
+ , Na+ , K+ , and Mg2+ ). A cluster of 78 T atoms (T referring to Si or Al atoms), comprising the zeolite Y super cavity coupled with the sodalite cage and three hexagonal prism units (Si(78- x ) Alx O129 H54 ; x = 1 or 2), was used in the calculations. The study was carried out using the ONIOM method. The high layer was computed at M062X/6-31++G(d,p), whereas the low layer was computed at the PM6 level of theory. The energy profile was obtained by single-point calculations at the M062X/6-31++G(d,p) for the entire structure. The first step studied was the adsorption of chloromethane on the zeolite, which involves an ion-dipole interaction between the metal cation and the chlorine atom. Then, two mechanistic pathways were investigated: one via formation of an adsorbed methoxide and the other involving a direct, one-step, nucleophilic attack of the water molecule to the adsorbed chloromethane. In all systems studied, the direct mechanism showed a lower energy of activation than the route involving the methoxide intermediate. The same behavior was also observed for chloromethane methanolysis to afford dimethyl ether on the metal-exchanged zeolites. The theoretical results are in agreement with the experiments of chloromethane hydrolysis over metal-exchanged zeolite Y, explaining the formation of dimethyl ether upon chloromethane methanolysis on zeolites of low or no acidity. The transition state for the direct route resembles a SN 2 process assisted by the surface, reinforcing the concept of zeolites as solid solvents, providing a polar nanoenvironment that favors and assists the formation of ionic transition states and intermediates., Competing Interests: The authors declare no competing financial interest., (© 2024 The Authors. Published by American Chemical Society.)- Published
- 2024
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31. Educational technologies for teaching hand hygiene: Systematic review.
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Fernandes DR, Santos BND, Guimarães CS, Ferreira EB, Margatho AS, Reis PEDD, Pittet D, and Silveira RCCP
- Subjects
- Humans, Health Personnel, Learning, Educational Technology, Hand Hygiene
- Abstract
Aim: To gather available scientific evidence on technologies used to teach hand hygiene to professional populations and lays involved in health care in the hospital setting. This systematic review was designed as proposed by Preferred Reporting Items for Systematic Reviews and Meta-Analysis, included studies reporting primary, original, quantitative research findings with no date limit and written in English, Spanish or Portuguese. The search was performed in the following electronic databases: Cochrane Library, Cumulative Index to Nursing and Allied Health Literature, Excerpta Medica dataBASE, Literatura Latino-Americana e do Caribe em Ciências da Saúde, US National Library of Medicine, Scopus, Web of Science, Google Scholar and ProQuest. The eligibility criteria were applied independently by two reviewers to select the studies, first by reading the titles and abstracts on the Rayyan platform and then by full text reading of the eligible studies. After a descriptive analysis, the studies were subjected to critical evaluation of their methodological quality using JBI tools., Results: Seven studies were included, addressing various methods for teaching hand hygiene using different technological resources, such as audiovisual electronic devices, videos, virtual reality, and gamification using tablets and smartphones, in different populations., Conclusion: Using technologies to teach hand hygiene considerably helps patients, visitors, and relatives in learning the procedures and efficiently improves hand hygiene compliance rates among healthcare professionals, creating evidence-based repetitive learning opportunities for patients and caregivers., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Fernandes et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
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32. Correlation between time on target and glycated hemoglobin in people with diabetes mellitus: systematic review.
- Author
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Lima RAD, Fernandes DR, Garcia RAC, Carvalho LADR, Silveira RCCP, and Teixeira CRS
- Subjects
- Humans, Glycated Hemoglobin, Blood Glucose Self-Monitoring, Blood Glucose, Diabetes Mellitus, Type 2
- Abstract
to analyze the correlation between time on target and glycated hemoglobin in people living with diabetes mellitus and carrying out continuous blood glucose monitoring or self-monitoring of capillary blood glucose., systematic review of etiology and risk based on JBI guidelines and reported according to Preferred Reporting Items for Systematic Reviews and Meta- Analyses, covering six databases and grey literature. The sample included 16 studies and methodological quality was assessed using JBI tools. Protocol registered in the Open Science Framework, available at https://doi.org/10.17605/OSF.IO/NKMZB., time on target (70-180 mg/dl) showed a negative correlation with glycated hemoglobin, while time above target (>180 mg/dl) showed a positive correlation. Correlation coefficients ranged between -0.310 and -0.869 for time on target, and between 0.66 and 0.934 for time above target. A study was carried out on a population that performed self-monitoring., there is a statistically significant correlation between time on target and time above target with glycated hemoglobin. The higher the proportion in the adequate glycemic range, the closer to or less than 7% the glycated hemoglobin will be. More studies are needed to evaluate this metric with data from self-monitoring of blood glucose.
- Published
- 2023
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33. Validity, Reproducibility, and Minimal Detectable Difference of the Functional Upper Extremity Function Test - Simplified Version - for Adults With Moderate to Severe Asthma and COPD.
- Author
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Correia NS, Oliveira JM, Fernandes DR, Feitosa DI, Pereira DM, do Amaral DP, Mesquita R, Pitta F, Dal Corso S, and Furlanetto KC
- Subjects
- Humans, Adult, Hand Strength, Reproducibility of Results, Upper Extremity, Pulmonary Disease, Chronic Obstructive, Asthma diagnosis
- Abstract
Background: Upper-limbs (ULs) functional tests which are valid and reliable for individuals with chronic respiratory disease (CRD) are scarce. The aim of this study was to investigate the intra-rater reproducibility, validity, minimal detectable difference (MDD), and learning effect of the Upper Extremity Function Test - simplified version (UEFT_S) functional test and to characterize its performance for adults with moderate-severe asthma and COPD., Methods: The UEFT_S was performed twice, and the number of elbow flexions in 20 s was the outcome. In addition, spirometry, 6-min walk test (6MWT), handgrip dynamometry (HGD), and usual and maximum timed-up-and-go tests (TUG_usual and TUG_max) were also performed., Results: Eighty-four individuals with moderate-severe CRD and 84 control individuals matched by anthropometric data were analyzed. Individuals with CRD presented better performance in the UEFT_S than controls ( P = .023). UEFT_S correlated significantly with HGD, TUG_usual, TUG_max, and 6MWT ( P < .047 for all). The test-retest intraclass correlation coefficient was 0.91 [0.86-0.94], and the MDD was 0.4%., Conclusions: The UEFT_S is a valid and reproducible tool to assess the functionality of the ULs in people with moderate-severe asthma and COPD. When applied in the modified form, the test can be considered simple, fast, and inexpensive, with an easy outcome to interpret., Competing Interests: The authors have disclosed no conflicts of interest., (Copyright © 2023 by Daedalus Enterprises.)
- Published
- 2023
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34. HLA-G expression in Merkel cell carcinoma and the correlation with Merkel cell polyomavirus infection.
- Author
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Parra LM, Sartori BGC, Fernandes DR, Fachin LRV, Nogueira MRS, Belone AFF, Nunes AJF, and Souza-Santana FC
- Subjects
- Humans, Aged, HLA-G Antigens, Retrospective Studies, Carcinoma, Merkel Cell genetics, Carcinoma, Merkel Cell pathology, Merkel cell polyomavirus genetics, Skin Neoplasms genetics, Polyomavirus Infections complications, Polyomavirus Infections genetics
- Abstract
Merkel cell carcinoma (MCC) is a rare aggressive neuroendocrine cutaneous carcinoma with a high mortality rate. The MCC etiology is not fully understood. Merkel cell-associated polyomavirus (MCPyV) was found in MCC patients, indicating a risk factor for the tumor. Caucasian, elderly, and immunocompromised individuals are more likely to develop this tumor. HLA-G consists of a non-classical class I (Ib) HLA molecule with an immunoregulatory function and was associated with tumor escape in different types of tumors, nonetheless, never been studied in MCC. The purpose of this study was to evaluate the HLA-G expression and also to detect the MCPyV in MCC patients and correlate it with the clinical course of the disease. Forty-five MCC patients were included in a retrospective study. Formalin-fixed paraffin-embedded cutaneous skin biopsies were used by immunohistochemistry and RT-PCR to verify the HLA-G expression and MCPyV infection. HLA-G expression was found in 7 (15.6%), while the presence of MCPyV was detected in 28 (62.2%) of the studied patients. No significant association was found between HLA-G expression and MCPyV infection (p = 0.250). The presence of MCPyV was associated with areas of low sunlight exposure (p = 0.042) and the HLA-G expression with progression to death (p = 0.038). HLA-G expression was detected in MCC patients, as well as the MCPyV presence was confirmed. These markers could represent factors with a possible impact on patient survival; however, further studies with a greater number of patients are needed, to better elucidate the possible role in disease progression., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Published
- 2023
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35. Genomic landscape of the SARS-CoV-2 pandemic in Brazil suggests an external P.1 variant origin.
- Author
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Perico CP, De Pierri CR, Neto GP, Fernandes DR, Pedrosa FO, de Souza EM, and Raittz RT
- Abstract
Brazil was the epicenter of worldwide pandemics at the peak of its second wave. The genomic/proteomic perspective of the COVID-19 pandemic in Brazil could provide insights to understand the global pandemics behavior. In this study, we track SARS-CoV-2 molecular information in Brazil using real-time bioinformatics and data science strategies to provide a comparative and evolutive panorama of the lineages in the country. SWeeP vectors represented the Brazilian and worldwide genomic/proteomic data from Global Initiative on Sharing Avian Influenza Data (GISAID) between February 2020 and August 2021. Clusters were analyzed and compared with PANGO lineages. Hierarchical clustering provided phylogenetic and evolutionary analyses of the lineages, and we tracked the P.1 (Gamma) variant origin. The genomic diversity based on Chao's estimation allowed us to compare richness and coverage among Brazilian states and other representative countries. We found that epidemics in Brazil occurred in two moments with different genetic profiles. The P.1 lineages emerged in the second wave, which was more aggressive. We could not trace the origin of P.1 from the variants present in Brazil. Instead, we found evidence pointing to its external source and a possible recombinant event that may relate P.1 to a B.1.1.28 variant subset. We discussed the potential application of the pipeline for emerging variants detection and the PANGO terminology stability over time. The diversity analysis showed that the low coverage and unbalanced sequencing among states in Brazil could have allowed the silent entry and dissemination of P.1 and other dangerous variants. This study may help to understand the development and consequences of variants of concern (VOC) entry., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Perico, De Pierri, Neto, Fernandes, Pedrosa, de Souza and Raittz.)
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- 2022
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36. Dressing to prevent surgical site infection in adult patients with cancer: a systematic review with meta-analysis.
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Dias TA, Fernandes DR, Dos Santos BN, Dos Reis PED, Margatho AS, and Silveira RCCP
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- Humans, Adult, Silver, Wound Healing, Bandages, Surgical Wound Infection etiology, Surgical Wound Infection prevention & control, Neoplasms surgery
- Abstract
Purpose: To identify the most effective dressing for application to surgical wounds with primary closure to prevent surgical site infection (SSI) in adult patients with cancer undergoing elective surgeries., Methods: This systematic review was based on the Preferred Reporting Items for Systematic Reviews and Meta-Analysis, with online searches conducted in the CINHAL, Cochrane Central, LILACS, PubMed, Scopus, Embase, Livivo, and Web of Science databases. An additional search was conducted in gray literature using Google Scholar. The risk of bias was assessed using RoB 2.0. The certainty of evidence was evaluated using the Grading of Recommendations Assessment and Development and Evaluation, and the results were synthesized in a descriptive manner and using meta-analysis., Results: Eleven randomized clinical trials were conducted to compare different types of dressing-silver dressing with absorbent dressing (n = 3), mupirocin dressing with paraffin/no dressing (n = 1), honey-based dressing with absorbent dressing (n = 1), vitamin E and silicone-containing dressing with absorbent dressing (n = 1), and negative pressure wound therapy with absorbent dressing (n = 4)-and compare the usage duration of absorbent dressing (n = 1). Nine trials presented a low risk of bias, and two were classified as having uncertain bias. Compared with absorbent dressing, silver dressing did not reduce the risk of developing any type of SSI in 894 clinical trial participants (risk relative RR: 0.72; 95% confidence interval [CI] [0.44, 1.17] p = 0.18). Compared with absorbent dressing, negative pressure wound therapy did not reduce the risk of developing any type of SSI in the 1041 participants of two clinical trials (RR 0.68; 95% CI [0.31, 1.26] p = 0.22). The certainty of evidence of the three meta-analyses was considered low or very low for the prevention of SSI. We believe that this low certainty of evidence can be improved by conducting new studies in the future., Conclusion: There is no evidence regarding which dressing is the most effective in preventing SSI in adult patients with cancer., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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- 2022
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37. Environmental Factors at the Periconceptional Period and the Occurrence of Cleft Lip and Palate in a Midwest Brazil Population: A Case-Control Study.
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Silvestre CMR, Silva AMC, Ferreira da Silva RCG, Bittencourt WS, Borba AM, Fernandes V, and da Silva CAL
- Subjects
- Male, Child, Female, Humans, Case-Control Studies, Brazil epidemiology, Risk Factors, Cleft Lip epidemiology, Cleft Lip etiology, Cleft Palate epidemiology, Cleft Palate etiology, Pesticides adverse effects
- Abstract
Objective: To investigate the association between periconceptional environmental exposures and the occurrence of cleft lips and palates., Methods: This case-control study analyzed 150 mothers of children with cleft lips and palates living in the same city as 250 mothers whose children did not present with this malformation (controls). Environmental exposure data were gathered through a questionnaire (Latin American Collaborative Study of Congenital Malformations methodology)., Results: Multivariate analysis revealed that monthly income below minimum wage, having another malformed child, other diseases in the first gestational trimester (urinary infection), use of pesticides in home gardens, and pesticide use in farms close to the home were risk factors associated with the malformation, whereas taking vitamins was a protective factor., Conclusion: Maternal and paternal exposure to pesticides is associated with cleft lip and palate in Mato Grosso State, Brazil., Competing Interests: Conflict of interest: None declared., (Copyright © 2022 American College of Occupational and Environmental Medicine.)
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- 2022
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38. Phenotypic and Genetic Studies of the Viral Lineage Associated with the Recent Yellow Fever Outbreak in Brazil.
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Furtado ND, Gómez MM, de Mello IS, Fernandes DR, and Bonaldo MC
- Subjects
- Amino Acids genetics, Animals, Brazil epidemiology, Disease Outbreaks, Mice, Phenotype, Yellow fever virus genetics, Yellow Fever epidemiology
- Abstract
Yellow fever virus (YFV) caused an outbreak in the Brazilian Southeast from 2016 to 2019, of the most significant magnitude since the 1900s. An investigation of the circulating virus revealed that most of the genomes detected in this period carried nine unique amino acid polymorphisms, with eight located in the non-structural proteins NS3 and NS5, which are pivotal for viral replication. To elucidate the effect of these amino acid changes on viral infection, we constructed viruses carrying amino acid alterations in NS3 and NS5, performed infection in different cells, and assessed their neurovirulence in BALB/c mice and infected AG129 mice. We observed that the residues that compose the YFV 2016-2019 molecular signature in the NS5 protein might have been related to an attenuated phenotype, and that the alterations in the NS3 protein only slightly affected viral infection in AG129 mice, increasing to a low extent the mortality rate of these animals. These results contributed to unveiling the role of specific naturally occurring amino acid changes in the circulating strain of YFV in Brazil.
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- 2022
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39. Phenotypic and Genetic Variability of Isolates of ZIKV-2016 in Brazil.
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Raphael LMS, de Mello IS, Gómez MM, Ribeiro IP, Furtado ND, Lima NS, Dos Santos AAC, Fernandes DR, da Cruz SOD, Damasceno LS, Brasil P, and Bonaldo MC
- Abstract
The possibility of a Zika virus epidemic resurgence requires studies to understand its mechanisms of pathogenicity. Here, we describe the isolation of the Zika virus from breast milk (Rio-BM1) and compare its genetic and virological properties with two other isolates (Rio-U1 and Rio-S1) obtained during the same epidemic period. Complete genomic analysis of these three viral isolates showed that they carry characteristics of the American isolates and belong to the Asian genotype. Furthermore, we detected eight non-synonymous single nucleotide variants and multiple nucleotide polymorphisms that reflect phenotypic changes. The new isolate, Rio-BM1, showed the lowest replication rates in mammalian cells, induced lower cell death rates, was more susceptible to treatment with type I IFN, and was less pathogenic than Rio-U1 in a murine model. In conclusion, the present study shows evidence that the isolate Rio-BM1 is more attenuated than Rio-U1, probably due to the impact of genetic alterations in the modulation of virulence. The results obtained in our in vitro model were consistent with the pathogenicity observed in the animal model, indicating that this method can be used to assess the virulence level of other isolates or to predict the pathogenicity of reverse genetic constructs containing other polymorphisms.
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- 2022
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40. Biological Characterization of Yellow Fever Viruses Isolated From Non-human Primates in Brazil With Distinct Genomic Landscapes.
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Furtado ND, Raphael LM, Ribeiro IP, de Mello IS, Fernandes DR, Gómez MM, Dos Santos AAC, Nogueira MDS, de Castro MG, de Abreu FVS, Martins LC, Vasconcelos PFDC, Lourenço-de-Oliveira R, and Bonaldo MC
- Abstract
Since the beginning of the XXI Century, the yellow fever virus (YFV) has been cyclically spreading from the Amazon basin to Brazil's South and Southeast regions, culminating in an unprecedented outbreak that started in 2016. In this work, we studied four YFV isolated from non-human primates obtained during outbreaks in the states of Rio Grande do Sul in 2008 (PR4408), Goiás (GO05), and Espírito Santo (ES-504) in 2017, and Rio de Janeiro (RJ 155) in 2019. These isolates have genomic differences mainly distributed in non-structural proteins. We compared the isolates' rates of infection in mammal and mosquito cells and neurovirulence in adult mice. RJ 155 and PR4408 YFV isolates exhibited higher infectivity in mammalian cells and neurovirulence in mice. In mosquito Aag2 cells, GO05 and PR4408 displayed the lowest proliferation rates. These results suggest that RJ 155 and PR4408 YFV isolates carry some genomic markers that increase infectivity in mammal hosts. From this characterization, it is possible to contribute to discovering new molecular markers for the virulence of YFV., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Furtado, Raphael, Ribeiro, de Mello, Fernandes, Gómez, dos Santos, Nogueira, de Castro, de Abreu, Martins, Vasconcelos, Lourenço-de-Oliveira and Bonaldo.)
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- 2022
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41. Use of technology for self-care in surgical wound infection surveillance: integrative review.
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Oliveira LP, Lucato ALS, Fernandes DR, Vieira LG, Santos BND, and Silveira RCCP
- Subjects
- Cross-Sectional Studies, Humans, Surgical Wound Infection epidemiology, Technology, Self Care, Text Messaging
- Abstract
Objectives: to synthesize knowledge about technology for self-care in surgical wound infection surveillance., Methods: integrative review conducted in CINAHL, Embase, LILACS, PubMed, Scopus and Web of Science databases with the descriptors "surgical wound infection" and "self-care", in addition to manual search in the references of the included studies. The selection and evaluation of the methodological quality of the studies and data collection were performed by two independent reviewers; conflicts were resolved by a third reviewer., Results: nine primary studies were included, published between 2011 and 2019. Six are cross-sectional, two are randomized clinical trials, and one is a case report. Mobile, text messaging, and imaging applications, computer software, assessments, and data storage capacity stand out., Conclusions: the technologies identified for self-care in surgical wound infection surveillance were the creation and use of mHealth and the use of health apps on mobile devices. Effective technologies in surveillance of surgical wound infection that enable rapprochement with the healthcare team, encourage a greater number of surgical wound assessments, enhance self-care actions, and decrease patient anxiety. Technology is also a monitored and recorded form of patient care, one of the main axes of infection surveillance.
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- 2022
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42. Ethanol extracts of mango seeds added to the diet of pigs increases antioxidant capacity of processed pork.
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Araújo LRS, Watanabe PH, Fernandes DR, Mello MCA, Maia IRO, Silva ÊCD, Owen RW, Nascimento GAJD, Trevisan MTS, and Freitas ER
- Subjects
- Animals, Antioxidants, Diet, Ethanol, Oxidation-Reduction, Plant Extracts pharmacology, Seeds, Swine, Mangifera, Pork Meat, Red Meat
- Abstract
Synthetic antioxidants (e.g.butylhydroxytoluene, BHT) are routinely used for to restrict oxidative processes of meat products, but they are implicated as harmful to the health of humans. Therefore natural alternatives, such as plant antioxidants, have been sought as replacements. Plant antioxidants when added to the diet can be incorporated into meat and reduce the need for the addition of synthetic antioxidants during processing. The objective of this study was to evaluate the effects of ethanol extracts of mango seeds (EEMS) in the diet of pigs on qualitative parameters and total antioxidant capacity of mortadella produced from these animals. Thirty-two pigs with an average 60 days of age were distributed among four treatments: control=no antioxidant; BHT=200ppm BHT; EEMS200=200ppm of EEMS and EEMS400=400ppm of EEMS. At 145 days of age the animals were slaughtered and loin was removed for the preparation of mortadella, which was analyzed during 90 days of storage at 4°C. A higher content of polyphenolic compounds and, total antioxidant capacity in mortadellas processed with meat of animals which consumed the EEMS400 ration after 60 and 90 days of storage was observed. EEMS polyphenolic antioxidants incorporated into pork through the diet results in an increase of total antioxidant capacity in the processed product.
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- 2021
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43. Postoperative complications in adult patients undergoing surgery with confirmed infection by SARS-CoV-2: An integrative review.
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Favaro E, Fernandes DR, Vieira LG, Margatho AS, Mendes KDS, and Silveira RCCP
- Subjects
- Humans, Intensive Care Units, Postoperative Complications epidemiology, Postoperative Complications etiology, Respiration, Artificial, COVID-19, SARS-CoV-2
- Abstract
Objective: to analyze the evidence available in the literature about postoperative complications in adult patients undergoing surgical procedures with confirmed infection by SARS-CoV-2., Method: an integrative literature review conducted in the CINAHL, EMBASE, LILACS, PubMed, Scopus and Web of Science databases, as well as in the gray literature. The references identified were exported to the EndNote manager and, subsequently, to the Rayyan web application for study selection. The stages of sampling, categorization of studies, evaluation of the studies included, interpretation of the results and knowledge synthesis were performed by two reviewers independently and in a masked manner. The data were analyzed descriptively., Results: of the 247 articles identified, 15 were selected to comprise this review. The prevalent postoperative complications in patients infected with SARS-CoV-2 were the following: cough, dyspnea and hypoxia, need for invasive mechanical ventilation or not, admission to the intensive care unit and death., Conclusion: the most reported postoperative complications in the studies evaluated were respiratory-related, followed by cardiovascular complications. The importance of preoperative screening for COVID-19 is highlighted, as well as of the monitoring and tracking of confirmed cases in the postoperative period, as these actions exert an impact on reducing the occurrence of complications related to SARS-CoV-2.
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- 2021
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44. Organic zinc and manganese and 25-hydroxycholecalciferol improves eggshell thickness in late-phase laying hens.
- Author
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Dos Santos EO, Freitas ER, Nepomuceno RC, Watanabe PH, Souza DH, Fernandes DR, de Abreu Freitas C, do Nascimento GAJ, Aguiar GC, and de Melo MCA
- Subjects
- Animal Feed analysis, Animals, Calcifediol pharmacology, Chickens, Diet veterinary, Dietary Supplements, Eggs, Female, Ovum, Zinc, Egg Shell, Manganese
- Abstract
The aim of this study was to evaluate the effects of organic micromineral zinc (Zn) and manganese (Mn) and 25-hydroxycholecalciferol supplementation in late-phase laying hens' diets on performance, egg quality, lipid stability of fresh and stored eggs, and bone quality. The treatments were a basal diet and diets supplemented with 32 mg Zn-Met/kg diet; 26 mg Mn-Met/kg diet; 32 mg Zn-Met/kg diet and 26 mg Mn-Met/kg diet; 1500 IU 25-hydroxycholecalciferol/kg diet; 32 mg Zn-Met/kg diet; 26 mg Mn-Met/kg diet; and 1500 IU 25-hydroxycholecalciferol/kg diet. On performance, the birds supplemented with organic manganese had the lowest feed intake. Regarding egg quality, the birds supplemented with Zn-Met and Mn-Met, with 25-hydroxycholecalciferol alone, and with Zn-Met, Mn-Met and 25-hydroxycholecalciferol presented a greater eggshell thickness than those receiving the basal diet. Lipid stability of the yolk varied only according to storage time. No effect of supplementation was observed on bone quality. Supplementation with Zn-Met and Mn-Met, or associated with 25-hydroxycholecalciferol, or 25-hydroxycholecalciferol alone, improved eggshell thickness in aged white layers. However, the associated or isolated supplementation with these nutrients did not influence performance, lipid stability of fresh and stored egg yolk or bone quality., (© 2021. The Author(s), under exclusive licence to Springer Nature B.V.)
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- 2021
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45. Physical exercise is effective for neuropsychiatric symptoms in Alzheimer's disease: a systematic review.
- Author
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Mendonça DCB, Fernandes DR, Hernandez SS, Soares FDG, Figueiredo K, and Coelho FGM
- Subjects
- Aged, Caregivers, Humans, Alzheimer Disease therapy, Exercise Therapy
- Abstract
Background: Neuropsychiatric symptoms are disorders frequently seen in Alzheimer's disease. These symptoms contribute to reduction of brain reserve capacity and, in addition, they present unfavorable implications, such as: poor prognosis for the disease, increased functional decline, increased burden on the caregiver and institutionalization. This scenario makes neuropsychiatric symptoms one of the biggest problems in Alzheimer's disease, and gives rise to a need for treatments focused on improving these symptoms. Sow progress in drug trials has led to interest in exploring non-pharmacological measures for improving the neuropsychiatric symptoms of Alzheimer's disease, such as physical exercise., Objective: To ascertain the effect of exercise on the neuropsychiatric symptoms of Alzheimer's disease and its implications., Methods: This was a systematic review of effective longitudinal research, conducted by searching for articles in the PubMed, Web of Science, CINAHL and Scopus electronic databases, from 2009 to 2019. Studies in which the sample consisted of elderly people aged 65 years old or over with a diagnosis of Alzheimer's disease were included. Initially 334 articles were identified. After exclusions, 21 articles remained to be read in full. From these, five articles fitted the eligibility criteria, and a further two articles were added through manual searches in the references of the articles found., Results: Out of the seven articles analyzed in this review, five studies revealed that physical exercise had a positive effect on the neuropsychiatric symptoms of Alzheimer's disease., Conclusion: This systematic review indicated that physical exercise is a favorable non-pharmacological means for attenuating the neuropsychiatric symptoms of elderly people with Alzheimer's disease, with special attention to aerobic exercises.
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- 2021
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46. Recovery of Synthetic Zika Virus Based on Rio-U1 Isolate Using a Genetically Stable Two Plasmid System and cDNA Amplification.
- Author
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de Mello IS, Fernandes DR, Furtado ND, Dos Santos AAC, Dos Santos MP, Ribeiro IP, Raphael LMS, Nogueira MDS, da Cruz SOD, Rocha ADS, Manso PPA, Pelajo-Machado M, and Bonaldo MC
- Abstract
In 2016, the world experienced the unprecedented Zika epidemic. The ZIKV emerged as a major human pathogen due to its association with the impairment of perinatal development and Guillain-Barré syndrome. The occurrence of these severe cases of Zika points to the significance of studies for understanding the molecular determinants of flavivirus pathogenesis. Reverse genetics is a powerful method for studying the replication and determinants of pathogenesis, virulence, and viral attenuation of flaviviruses, facilitating the design of vaccines and therapeutics. However, the main hurdle in the development of infectious clones is the instability of full-length cDNA in Escherichia coli . Here, we described the development of a genetically stable and efficient infectious clone based on the ZIKV Rio-U1 isolated in the 2016 epidemic in Brazil. The employed strategy consisted of cloning the viral cDNA genome into two stable plasmid subclones and obtaining a high-quality cDNA template with increment in DNA mass for in vitro transcription by PCR amplification. The strategy for developing a ZIKV infectious cDNA clone designed in this study was successful, yielding a replicative and efficient clone-derived virus with high similarities with its parental virus, Rio-U1, by comparison of the proliferation capacity in mammal and insect cells. The infection of AG129 immunocompromised mice caused identical mortality rates, with similar disease progression and morbidity in the animals infected with the parental and the cDNA-derived virus. Histopathological analyses of mouse brains infected with the parental and the cDNA-derived viruses revealed a similar pathogenesis degree. We observed meningoencephalitis, cellular pyknosis, and neutrophilic invasion adjacent to the choroid plexus and perivascular cuffs with the presence of neutrophils. The developed infectious clone will be a tool for genetic and functional studies in vitro and in vivo to understand viral infection and pathogenesis better., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 de Mello, Fernandes, Furtado, dos Santos, dos Santos, Ribeiro, Raphael, Nogueira, da Cruz, Rocha, Manso, Pelajo-Machado and Bonaldo.)
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- 2021
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47. Dietary ethanol extract of mango increases antioxidant activity of pork.
- Author
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Araújo LRS, Watanabe PH, Fernandes DR, Maia IRO, Silva ECD, Pinheiro RRS, Melo MCA, Santos EOD, Owen RW, Trevisan MTS, and Freitas ER
- Subjects
- Animal Feed analysis, Animals, Antioxidants, Diet veterinary, Ethanol, Meat analysis, Plant Extracts pharmacology, Swine, Mangifera, Pork Meat, Red Meat
- Abstract
Ethanol extract of mango seeds (EEMS) are composed of several polyphenolic compounds with considerable in vitro antioxidant activity that can be used in pig feed and may contribute positively to meat quality characteristics. The aim of this study was to evaluate the effect of EEMS as a source of antioxidants in growing-finishing pig diets on meat quality, lipid stability, sulfhydryl groups non-proteinaceous (SG-NP), total phenolic compounds, total antioxidant potential and total antioxidant activity of meat after 1 and 7 days of refrigeration storage. Thirty-two (60-day-old) barrows, weighing 20.20 ± 1.34 kg, were used in a randomized block design consisting of eight animals with four treatment regimens. Treatments consisted of: Control = no dietary antioxidant; butylated hydroxytoluene (BHT) = diet with 200 ppm BHT; EEMS200 = diet with 200 ppm EEMS; EEMS400 = diet with 400 ppm EEMS. At 145 days of age and average weight of 95.47 ± 6.19 kg, the animals were slaughtered and loin samples were collected and frozen before for qualitative analysis and evaluation of the effect of subsequent storage for 1 or 7 days at 8 °C on lipid stability, SG-NP, phenolic compounds, total antioxidant capacity and total antioxidant activity Meat from animals fed EEMS400 diet showed lower cooking loss (P < 0.0001) and higher non-protein sulfhydryl groups, phenolic compounds and total antioxidant activity at both 1 and 7 days of storage (P < 0.0001) compared to the other treatments. Greater antioxidant capacity was observed at 1 day storage in the meat of animals that consumed EEMS regardless of concentration when compared to the control group (P < 0.01). The dietary inclusion of EEMS to pig diets is more effective at 400 ppm in improving meat quality after cooking and antioxidant parameters of pork., (Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.)
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- 2021
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48. Effectiveness of interventions for the prevention of occupational violence against professionals in health services: a protocol for a systematic review.
- Author
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Okubo CVC, Silveira RCCP, Galdino MJQ, Fernandes DR, Moreira AAO, and Martins JT
- Subjects
- Child, Health Services, Humans, Meta-Analysis as Topic, Review Literature as Topic, Violence prevention & control, Health Personnel, Research Design
- Abstract
Introduction: Occupational violence affects several categories of workers; however, the health sector category has been considered at a high risk, exposing workers to physical and psychological abuse. Thus, occupational violence has decreased the quality of care in health service. This review aims to evaluate the effectiveness of interventions for the prevention and reduction of occupational violence against health professionals., Methods and Analysis: This protocol is consistent with the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols. Searches will be conducted in PubMed, Embase, Cochrane Library, LILACS, Web of Science, Scopus, CINAHL and LIVIVO along with a comprehensive review of grey literature. The search will be conducted on August 1 st 2020, without language and time restrictions. Following the eligibility criteria, two independent reviewers will select the titles and abstracts and subsequently screen the full articles. If necessary, a third reviewer will assess any disagreements. All references will be imported into EndNote, and any duplicates will be removed. The data will be extracted using an extraction-based form from Cochrane. Statistical analyses will be performed using the software Cochrane Review Manager, and a meta-analysis will be performed if possible for the statistical combination of at least two studies. The risk of bias of the randomised clinical trials will be evaluated by the Risk of Bias tool from Cochrane, and the risk of bias of the non-randomised intervention studies will be evaluated using the Downs and Black scale. The quality of the evidence and strength of the classification recommendations will be assessed by the Grading of Recommendations, Assessment, Development and Evaluation., Ethics and Dissemination: This review will not evaluate individual patient information and therefore does not require ethical approval. The results will be disseminated through publications in peer-reviewed journals, presentations at conferences and the doctoral thesis of the leading author., Prospero Registration Number: CRD42018111383., Competing Interests: Competing interests: None declared., (© Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.)
- Published
- 2020
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49. Black bone syndrome in broilers fed ethanolic extract of mango seeds.
- Author
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de Melo MCA, Gomes HM, Faria NNP, Freitas ER, Watanabe PH, Watanabe GCA, Souza DH, and Fernandes DR
- Subjects
- Animals, Bone Diseases chemically induced, Bone Diseases pathology, Dose-Response Relationship, Drug, Male, Plant Extracts chemistry, Poultry Diseases chemically induced, Seeds chemistry, Bone Diseases veterinary, Chickens, Cooking methods, Mangifera chemistry, Meat analysis, Plant Extracts adverse effects, Poultry Diseases pathology
- Abstract
This study aimed to evaluate the incidence of black bone syndrome (BBS) in broiler chickens fed with ethanolic extract of mango seed (EEMS). A total of 504 one-day-old male broilers were used in a completely randomised design assigned with 7 experimental diets and 6 replicates of 12 broilers per experimental plot. The experimental diets consisted of: diet without addition of synthetic antioxidant; diet with addition of synthetic antioxidant (200 ppm); and 5 levels of EEMS: 200 ppm, 400 ppm, 600 ppm, 800 ppm, and 1,000 ppm. Two methods of cooking (roasted and boiled) were used to prepare thigh samples. According to the results, the diets did not significantly influence the performance of the broilers. BBS incidence was higher in broilers fed a diet without antioxidants and was reduced with EEMS dietary inclusion, with the lowest incidence occurring with the inclusion of 1,000 ppm. The synthetic antioxidant butylated hydroxytoluene in the diet promoted a significantly higher BBS incidence than that obtained with 800 and 1,000 ppm EEMS and did not differ from the other diets. Of the cooking methods, a higher BBS incidence was observed for the boiled method. For the meat coloration and bone parameters, there were no significant interactions between the factors, diets and cooking methods. There was a linear reduction in the darkening score and linear increase in the luminosity (L∗) of the meat with increasing EEMS in the diet. With regard to the cooking method, the boiled thighs had lower luminosity (L∗), higher parameter a∗, and lower parameter b∗ values because of more pronounced meat darkening. The roasted bones were less heavy, dense, and flexible. A negative correlation was observed between the degree of darkening of the meat that characterizes the BBS with the luminosity (L∗) and intensity of yellow. We concluded that the addition of EEMS contributes to a reduced darkening of meat that characterises the BBS and recommend the dietary inclusion of 1,000-ppm EEMS., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
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50. Hand hygiene: knowledge and skill of caregivers in the hematopoietic stem cell transplantation.
- Author
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Fernandes DR, Braga FTMM, Silveira RCCP, and Garbin LM
- Subjects
- Adult, Aged, Brazil, Caregivers psychology, Caregivers statistics & numerical data, Clinical Competence statistics & numerical data, Cross-Sectional Studies, Female, Hand Hygiene methods, Hand Hygiene statistics & numerical data, Hematopoietic Stem Cell Transplantation methods, Humans, Male, Middle Aged, Prospective Studies, Surveys and Questionnaires, Caregivers standards, Clinical Competence standards, Hand Hygiene standards, Hematopoietic Stem Cell Transplantation nursing
- Abstract
Objective: to identify the knowledge and ability of caregivers on hand hygiene in hematopoietic stem cell transplantation., Method: a prospective cross-sectional study. A questionnaire was used to identify the knowledge and it was observed the hygiene technique performance. Data were collected in two units (autologous and allogeneic transplant)., Results: the 37 participants recognized the importance of hand hygiene and 95.5% related to removal of dirt or infection prevention. 91.9% stated that it was important to clean their hands with soap and water when entering and leaving the room, and 64.9% understood that it was necessary to apply alcoholic solution after washing. On average, the caregivers scored 6.16 steps, when demonstrating the washing technique and 3.91 steps in the friction with alcoholic solution., Conclusion: although they recognize the importance of the procedure, there are deficits related to the moment and the correct way of doing it, evidencing the need for strategies aimed at improving this process.
- Published
- 2019
- Full Text
- View/download PDF
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