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Your search keyword '"Ferguson, Cristin M."' showing total 8 results
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8 results on '"Ferguson, Cristin M."'

4. HIF_SupplementaryInformation_Revised – Supplemental material for Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus

Author

Stone, Austin V., Loeser, Richard F., Callahan, Michael F., McNulty, Margaret A., Long, David L., Raghunatha R. Yammani, Bean, Sara, Kadie Vanderman, Chubinskaya, Susan, and Ferguson, Cristin M.

Subjects
FOS: Clinical medicine, FOS: Biological sciences, 110323 Surgery, 110604 Sports Medicine, FOS: Health sciences, 69999 Biological Sciences not elsewhere classified, 110314 Orthopaedics
Abstract

Supplemental material, HIF_SupplementaryInformation_Revised for Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus by Austin V. Stone, Richard F. Loeser, Michael F. Callahan, Margaret A. McNulty, David L. Long, Raghunatha R. Yammani, Sara Bean, Kadie Vanderman, Susan Chubinskaya and Cristin M. Ferguson in CARTILAGE

Published
2021
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5. Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus

Author

Stone, Austin V., primary, Loeser, Richard F., additional, Callahan, Michael F., additional, McNulty, Margaret A., additional, Long, David L., additional, Yammani, Raghunatha R., additional, Bean, Sara, additional, Vanderman, Kadie, additional, Chubinskaya, Susan, additional, and Ferguson, Cristin M., additional

Published
2020
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6. Role of the Hypoxia-Inducible Factor Pathway in Normal and Osteoarthritic Meniscus and in Mice after Destabilization of the Medial Meniscus

Author

Stone, Austin V., Loeser, Richard F., Callahan, Michael F., McNulty, Margaret A., Long, David L., Yammani, Raghunatha R., Bean, Sara, Vanderman, Kadie, Chubinskaya, Susan, and Ferguson, Cristin M.

Abstract

Objective Meniscus injury and the hypoxia-inducible factor (HIF) pathway are independently linked to osteoarthritis pathogenesis, but the role of the meniscus HIF pathway remains unclear. We sought to identify and evaluate HIF pathway response in normal and osteoarthritic meniscus and to examine the effects of Epas1 (HIF-2α) insufficiency in mice on early osteoarthritis development.Methods Normal and osteoarthritic human meniscus specimens were obtained and used for immunohistochemical evaluation and cell culture studies for the HIF pathway. Meniscus cells were treated with pro-inflammatory stimuli, including interleukins (IL)-1β, IL-6, transforming growth factor (TGF)-α, and fibronectin fragments (FnF). Target genes were also evaluated with HIF-1α and HIF-2α (Epas1) overexpression and knockdown. Wild-type (n= 36) and Epas1+/−(n= 30) heterozygous mice underwent destabilization of the medial meniscus (DMM) surgery and were evaluated at 2 and 4 weeks postoperatively for osteoarthritis development using histology.Results HIF-1α and HIF-2α immunostaining and gene expression did not differ between normal and osteoarthritic meniscus. While pro-inflammatory stimulation significantly increased both catabolic and anabolic gene expression in the meniscus, HIF-1α and Epas1 expression levels were not significantly altered. Epas1 overexpression significantly increased Col2a1 expression. Both wild-type and Epas1+/−mice developed osteoarthritis following DMM surgery. There were no significant differences between genotypes at either time point.Conclusion The HIF pathway is likely not responsible for osteoarthritic changes in the human meniscus. Additionally, Epas1 insufficiency does not protect against osteoarthritis development in the mouse at early time points after DMM surgery. The HIF pathway may be more important for protection against catabolic stress.

Published
2021
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8. Variability in Patellofemoral Alignment Measurements on MRI: Influence of Knee Position.

Author

Marquez-Lara A, Andersen J, Lenchik L, Ferguson CM, and Gupta P

Subjects
Adult, Anatomic Landmarks, Female, Healthy Volunteers, Humans, Image Processing, Computer-Assisted, Male, Range of Motion, Articular, Magnetic Resonance Imaging methods, Patellofemoral Joint anatomy & histology, Patellofemoral Joint diagnostic imaging
Abstract

Objective: The purpose of this study was to determine the variability of distances between four distinct anatomic landmarks used for the evaluation of patellofemoral alignment while implementing controlled changes in patient positioning during MRI., Materials and Methods: Limited MRI was performed of 12 knees in healthy volunteers (10 men, two women; mean age, 28 years) with the knees in four different positions. The four landmarks used were TT (the most anterior point of the osseous tibial tubercle), TG (the nadir of the cartilaginous trochlear groove), PT (the center of the patellar tendon insertion on the tibia), and PCL (the medial border of the posterior cruciate ligament at its insertion along the posterior tibia). TT-TG, PT-TG, TT-PCL, and PT-PCL distances were measured on the MR images. Each distance was measured with the knee at maximum patient comfort (rest), full extension, 15° of flexion, and 30° of flexion. Linear mixed models with random intercepts were implemented to determine variability between measurements and knee position., Results: In general, measurements based on anatomic landmark and knee position varied greatly. The greatest variability in different knee positions was seen in mean TT-TG and PT-TG distances ± SD (TT-TG: rest, 18.1 ± 7.9 mm; full extension, 17.3 ± 5.3 mm; 15° of flexion, 11.4 ± 5.7 mm; 30° of flexion, 11.7 ± 6.0 mm; intraclass correlation coefficient [ICC] = 0.539; PT-TG: rest, 18.1 ± 6.3 mm; full extension, 17.9 ± 4.7 mm; 15° of flexion, 13.2 ± 5.2 mm; 30° of flexion, 11.8 ± 4.3 mm; ICC = 0.633). In contrast, knee position did not significantly affect distances for TT-PCL and PT-PCL (TT-PCL: rest, 23.5 ± 6.8 mm; full extension, 20.5 ± 5.5 mm; 15° of flexion, 22.8 ± 6.2 mm; 30° of flexion, 22.8 ± 6.7 mm; ICC = 0.484; PTPCL: rest, 23.4 ± 5.3 mm; full extension, 21.5 ± 4.5 mm; 15° of flexion, 22.3 ± 4.3 mm; 30° of flexion, 23.1 ± 4.8 mm; ICC = 0.509)., Conclusion: On MRI, TT-PCL and PT-PCL measurements have significantly less variability compared with TT-TG and PT-TG measurements, regardless of knee position. Although further studies are warranted, the use of TT-PCL and PT-PCL measurements may offer more reliable assessment of tibial tubercle lateralization and patellofemoral alignment.

Published
2017
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