5 results on '"Elisabeth Höring"'
Search Results
2. Dual targeting of MCL1 and NOXA as effective strategy for treatment of mantle cell lymphoma
- Author
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Simon Heine, Walter E. Aulitzky, Lea Schaaf, Gaël Roué, Matthias Vöhringer, Dolors Colomer, Elias Campo, Anna Esteve-Arenys, Arnau Montraveta, Markus Kleih, Elisabeth Höring, German Ott, and Heiko van der Kuip
- Subjects
0301 basic medicine ,Dual targeting ,Apoptosis ,Pyridinium Compounds ,Lymphoma, Mantle-Cell ,Mice, SCID ,Cyclic N-Oxides ,Lactones ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Line, Tumor ,hemic and lymphatic diseases ,medicine ,Animals ,Humans ,MCL1 ,Enzyme Inhibitors ,Dinaciclib ,Orlistat ,biology ,Indolizines ,Hematology ,Bridged Bicyclo Compounds, Heterocyclic ,medicine.disease ,Cyclin-Dependent Kinases ,Cell biology ,Fatty acid synthase ,030104 developmental biology ,Cell Death Induction ,Proto-Oncogene Proteins c-bcl-2 ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Myeloid Cell Leukemia Sequence 1 Protein ,Female ,Mantle cell lymphoma ,CDK inhibitor - Abstract
Imbalances in the composition of BCL2 family proteins contribute to tumourigenesis and therapy resistance of mantle cell lymphoma (MCL), making these proteins attractive therapy targets. We studied the efficiency of dual targeting the NOXA/MCL1 axis by combining fatty acid synthase inhibitors (NOXA stabilization) with the CDK inhibitor Dinaciclib (MCL1 reduction). This combination synergistically induced apoptosis in cell lines and primary MCL cells and led to almost complete inhibition of tumour progression in a mouse model. Apoptosis was NOXA-dependent and correlated with the NOXA/MCL1 ratio, highlighting the importance of the NOXA/MCL1 balance for effective cell death induction in MCL.
- Published
- 2017
3. B-cell receptor-driven MALT1 activity regulates MYC signaling in mantle cell lymphoma
- Author
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Jan Molinsky, Annette M. Staiger, Nicole Dzyuba, Michael Grau, Margot Thome, Pavel Klener, Hannelore Madle, Alexandar Tzankov, Wolfgang E. Berdel, Elisabeth Höring, Georg Lenz, German Ott, Wendan Xu, Marek Trneny, Daniel Krappmann, Ioannis Anagnostopoulos, Reiner Siebert, Martin Dreyling, Peter Lenz, Wolfram Klapper, Niklas Vogt, Sietse M. Aukema, Tabea Erdmann, Gisela Schimmack, Korinna Jöhrens, Eva Hoster, Mélanie Juilland, Andreas Rosenwald, Kristian Erdmann, and Beiying Dai
- Subjects
0301 basic medicine ,Cell Survival ,Immunology ,B-cell receptor ,Receptors, Antigen, B-Cell ,Lymphoma, Mantle-Cell ,Biology ,Biochemistry ,Proto-Oncogene Proteins c-myc ,03 medical and health sciences ,Mice ,Downregulation and upregulation ,hemic and lymphatic diseases ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Regulation of gene expression ,Cell Death ,breakpoint cluster region ,NF-kappa B ,Cell Biology ,Hematology ,NFKB1 ,medicine.disease ,Neoplasm Proteins ,Gene expression profiling ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Mucosa-Associated Lymphoid Tissue Lymphoma Translocation 1 Protein ,Caspases ,Cancer research ,Heterografts ,Mantle cell lymphoma ,Signal transduction ,Signal Transduction - Abstract
Mantle cell lymphoma (MCL) is a mature B-cell lymphoma characterized by poor clinical outcome. Recent studies revealed the importance of B-cell receptor (BCR) signaling in maintaining MCL survival. However, it remains unclear which role MALT1, an essential component of the CARD11-BCL10-MALT1 complex that links BCR signaling to the NF-κB pathway, plays in the biology of MCL. Here we show that a subset of MCLs is addicted to MALT1, as its inhibition by either RNA or pharmacologic interference induced cytotoxicity both in vitro and in vivo. Gene expression profiling following MALT1 inhibition demonstrated that MALT1 controls an MYC-driven gene expression network predominantly through increasing MYC protein stability. Thus, our analyses identify a previously unappreciated regulatory mechanism of MYC expression. Investigating primary mouse splenocytes, we could demonstrate that MALT1-induced MYC regulation is not restricted to MCL, but represents a common mechanism. MYC itself is pivotal for MCL survival because its downregulation and pharmacologic inhibition induced cytotoxicity in all MCL models. Collectively, these results provide a strong mechanistic rationale to investigate the therapeutic efficacy of targeting the MALT1-MYC axis in MCL patients.
- Published
- 2016
4. Notch1 Signaling in NOTCH1-Mutated Mantle Cell Lymphoma Depends on DLL4 and Is a Potential Target for Specific Antibody Therapy
- Author
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Berta Colom Sanmarti, Walter E. Aulitzky, Heiko van der Kuip, Elias Campo, Dolors Colomer, Silvia Xargay-Torrent, Elisabeth Höring, and Mónica López-Guerra
- Subjects
JAG2 ,JAG1 ,Mutation ,biology ,Immunology ,Notch signaling pathway ,Cell Biology ,Hematology ,Cell cycle ,medicine.disease_cause ,Biochemistry ,Molecular biology ,Ubiquitin ligase ,03 medical and health sciences ,0302 clinical medicine ,Cyclin D1 ,hemic and lymphatic diseases ,030220 oncology & carcinogenesis ,embryonic structures ,cardiovascular system ,biology.protein ,medicine ,HES1 ,030215 immunology - Abstract
Mantle cell lymphoma (MCL) is a rare B-cell-neoplasm with an aggressive clinical course characterized by the hallmark translocation t(11;14)(q13;q32) resulting in overexpression of cyclin D1. Furthermore, genomic profiling revealed numerous secondary genetic alterations and recurrent mutations contributing to MCL pathogenesis. Among them, mutations in the NOTCH1 gene have been described with a frequency of ~10% and are associated with significantly shorter survival rates. Therefore, further investigation of the biological impact of this mutation in MCL and its potential as a target for a specific inhibitory antibody therapy is of great interest. Here we investigated the role of the Notch receptor ligands Jagged1 (JAG1), Jagged2 (JAG2), Delta-like canonical Notch ligand 1 (DLL1) and Delta-like canonical Notch ligand 4 (DLL4) in activating the Notch1-signaling pathway in NOTCH1-mutated Mino and NOTCH1-wt Jeko1 MCL cell lines and evaluated the effects of the novel and specific monoclonal Notch1-antibody OMP-52M51. Mino cells are characterized by a single nucleotide substitution in exon 34 of the NOTCH1 gene, leading to truncation of the C-terminal PEST-domain. These mutations are known to remove the recognition site from the ubiquitin ligase degradation complex, resulting in increased stability and transcriptional activity of Notch1 upon ligand-stimulation. We confirmed the predicted truncation of Notch1 in Mino cell lysates and demonstrated that the expression of cleaved Notch1 was potently stimulated by recombinant DLL4 protein. In contrast, in the NOTCH1-wt cell line Jeko1, no stable overexpression of cleaved Notch1 could be observed upon any ligand-stimulation. Treatment of the NOTCH1-mutated cells with OMP-52M51 effectively prevented DLL4-dependent activation of Notch1 and suppressed transcriptional induction of well-known Notch1-target genes HES1 (hairy and enhancer of-split 1), DTX1 (deltex E3 ubiquitin ligase 1) and c-MYC as observed by real-time quantitative PCR. Gene expression profiling furthermore revealed that OMP-52M51 significantly impeded DLL4-induced upregulation of numerous genes involved in lymphoid biology and lymphomagenesis, as well as genes related to the cell cycle and the MAP-Kinase signaling pathway. Western blot analysis confirmed Notch1-antibody-mediated abrogation of enhanced expression of p-ERK and p-MEK upon DLL4-ligand-stimulation. Further investigations of functional effects revealed inhibitory effects of OMP-52M51 on enhanced tumor cell migration of DLL4-stimulated cells. As the DLL4-Notch-axis is known to be involved in regulating angiogenesis, we investigated the impact of activating NOTCH1-mutations in MCL on HUVEC tube formation and observed increased vessel sprouting upon DLL4-stimulation that could be abolished by treatment with OMP-52M51. Importantly, all these effects were specific for NOTCH1-mutated cells and did not occur in the NOTCH1-wt cell line Jeko1. In conclusion, we demonstrate for the first time that DLL4 is the most important ligand to activate Notch1 in MCL harbouring NOTCH1-mutations in the PEST-domain. Our findings indicate that enhanced Notch1 activity promotes a more aggressive behavior of the disease and specific antibody-inhibition of the Notch1-signaling pathway might provide an interesting therapeutic alternative for a subset of MCL patients, warranting further investigation. Disclosures No relevant conflicts of interest to declare.
- Published
- 2016
5. Dual Targeting of NOXA/MCL-1 Synergistically Induces Cell Death in Mantle Cell Lymphoma (MCL) Cells
- Author
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Heiko van der Kuip, Kleih Markus, Walter E. Aulitzky, Elisabeth Höring, German Ott, Christine Bayha, and Matthias Voehringer
- Subjects
Programmed cell death ,Low protein ,biology ,Bortezomib ,Immunology ,Cell Biology ,Hematology ,Biochemistry ,Cell biology ,chemistry.chemical_compound ,chemistry ,Cyclin-dependent kinase ,Apoptosis ,hemic and lymphatic diseases ,biology.protein ,Proteasome inhibitor ,medicine ,Dinaciclib ,Helenalin ,medicine.drug - Abstract
Mantle cell lymphoma (MCL) cells are characterized by a discrepancy between high mRNA and low protein levels of the pro-apoptotic BH3-only protein NOXA. Modulation of NOXA protein expression has been described as a major mechanism of cell death induction in MCL cells. However, the efficiency of induction of apoptosis at lower concentrations is limited despite effective stabilization of NOXA. We therefore investigated whether the main binding partner of NOXA, the anti-apoptotic protein MCL-1 is co-regulated by these agents and whether dual targeting of NOXA and MCL-1 could be a promising strategy to enhance effectiveness of cell death induction in MCL cell lines. Screening a panel of compounds supposed to lead to NOXA stabilization in MCL, we identified the proteasome inhibitor Bortezomib, the fatty acid synthase inhibitor Orlistat and the ROS inducing agents Helenalin, a sesquesterpenone lactone from Arnica and the naphtoquinone derivative Menadione to kill MCL cells very effectively in a NOXA -dependent manner. Investigating the NOXA-/MCL-1-protein expression upon treatment with these agents, we could observe that at lower, sublethal concentrations of Bortezomib and Orlistat, NOXA protein was increased to a certain extent but also the anti-apoptotic MCL-1 was highly induced, counteracting induction of apoptosis. From this observation it has to be concluded that MCL-1 limits the apoptotic activity of NOXA stabilizing agents and dual targeting of MCL-1 and NOXA is required for optimal killing of MCL cells. In search for MCL-1 regulating agents we could identify the cdk-inhibitor Dinaciclib to be the most effective one. This compound rapidly downregulates Mcl-1 protein in a dose- and time-dependent manner presumably due to cdk 9-mediated inhibition of phosphorylation of the RNA-Polymerase II-subunit RPB1. To study the efficiency of dual targeting of NOXA/MCL-1 in killing of MCL cells we combined sublethal doses of Dinaciclib with NOXA stabilizing agents and observed a synergistic induction of apoptosis in MCL cells. Western Blot analysis showed that combination treatment decreased Mcl-1 and increased NOXA protein expression compared to single-agents. It could be shown that induction of cell death by treatment with the combined agents depends on NOXA as transfection of cells with NOXA-siRNA rescued cells from induction of apoptosis. Cell death upon treatment with Dinaciclib and ROS-inducing agents Helenalin and Menadione could furthermore be rescued by preincubation with the antioxidant GSH. Interestingly, combined treatment of cells with Orlistat and Dinaciclib killed most effectively when Dinaciclib was added for 8 hours after 16 hours of preincubation with the NOXA stabilizing agent. This observation contributes to the hypothesis that stabilization of NOXA protein leads to priming of MCL cells to induction of apoptosis by pharmacological downregulation of Mcl-1 with Dinaciclib. In summary, the NOXA-MCL-1 balance is critical for survival of MCL cells. Dual targeting of MCL-1 and NOXA efficiently kills MCL cells and appears to be of particular importance especially at lower concentrations of these compounds. These culture conditions most likely resemble the limited exposure after in vivo treatment. Therefore the combination of NOXA stabilizing agents with Dinaciclib appears to be a promising strategy to be tested in clinical trials. Disclosures No relevant conflicts of interest to declare.
- Published
- 2015
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