Search

Your search keyword '"Di Ilio, Carmine"' showing total 17 results
Start Over Author "Di Ilio, Carmine" Publication Year Range Last 10 years
17 results on '"Di Ilio, Carmine"'

12. Cryopreservation influence in the WJCs Proteome

Author

Marchisio, Marco, Pierdomenico, Laura, Lanuti, Paola, Ercolino, Eva, Bologna, Giuseppina, Grifone, Giovanna, Angelucci, Stefania, Di Ilio, Carmine, and Miscia, Sebastiano

Subjects
Wharton’s jelly cells, Cryopreservation, Proteome analysis
Abstract

Cryopreservation is the only mode of long-term storage of viable cells and tissues for cellular therapy, stem cell transplantation and/or tissue engineering. However the freeze-thaw process strongly contributes to cell and tissue damage with several mechanism, including oxidative stress, intracellular ice formation (IIF) dependent cell injury and altered physical cellular properties, i.e. osmotic and ion homeostasis. Our previous proteomics investigation was carried on Wharton’s jelly cells (WJCs), fibroblastlike cells with similar properties to mesenchymal stem cells, therefore a rich source of primitive cells to be potentially used in regenerative medicine. The aim of the present work was to investigate molecular changes that occur in WJCs proteome at different culture conditions (freshly and post-frozen cell preparations) and to elucidate possible mechanism involved in maintaining active proliferation and maximal cellular plasticity in order to optimize in vitro culturing procedure. To analyze changes in protein expression of WJCs we performed a comparative proteomic analysis (2DE followed by MALDI–TOF MS) between fresh and post-frozen cell culturing. WJCs postfrozen showed a qualitative and quantitative changes compared to cells from fresh preparation, expressing proteins involved in replication, cellular defense mechanism and metabolism, that ensure freeze-thaw survival. However, further investigations are needed to clarify the biological mechanisms involved in maintaining active proliferation, plasticity, multipotency cell during in vitro expansion, Italian Journal of Anatomy and Embryology, Vol 119, No 1 (Supplement) 2014

Published
2015
Full Text
View/download PDF

13. Evaluation of antibacterial and antibiofilm mechanisms by usnic acid against methicillin-resistant Staphylococcus aureus

Author

Pompilio, Arianna, primary, Riviello, Antonella, additional, Crocetta, Valentina, additional, Di Giuseppe, Fabrizio, additional, Pomponio, Stefano, additional, Sulpizio, Marilisa, additional, Di Ilio, Carmine, additional, Angelucci, Stefania, additional, Barone, Luana, additional, Di Giulio, Andrea, additional, and Di Bonaventura, Giovanni, additional

Published
2016
Full Text
View/download PDF

17. Unraveling the molecular repertoire of tears as a source of biomarkers: beyond ocular diseases.

Author

Pieragostino D, D'Alessandro M, di Ioia M, Di Ilio C, Sacchetta P, and Del Boccio P

Subjects
Animals, Humans, Metabolomics methods, Biomarkers metabolism, Eye Diseases diagnosis, Eye Diseases metabolism, Eye Proteins analysis, Proteome analysis, Proteomics methods, Tears chemistry
Abstract

Proteomics and metabolomics investigations of body fluids present several challenges for biomarker discovery of several diseases. The search for biomarkers is actually conducted in different body fluids, even if the ideal biomarker can be found in an easily accessible biological fluid, because, if validated, the biomarker could be sought in the healthy population. In this regard, tears could be considered an optimum material obtained by noninvasive procedures. In the past years, the scientific community has become more interested in the study of tears for the research of new biomarkers not only for ocular diseases. In this review, we provide a discussion on the current state of biomarkers research in tears and their relevance for clinical practice, and report the main results of clinical proteomics studies on systemic and eye diseases. We summarize the main methods for tear samples analyses and report recent advances in "omics" platforms for tears investigations. Moreover, we want to take stock of the emerging field of metabolomics and lipidomics as a new and integrated approach to study protein-metabolites interplay for biomarkers research, where tears represent a still unexplored and attractive field., (© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results