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7. Apple stem grooving capillovirus : pliant pathogen and its potential as a tool in functional genomics and effective disease management

Author

Vats, Gourav, primary, Sharma, Vasudha, additional, Noorani, Salik, additional, Rani, Asha, additional, Kaushik, Naveen, additional, Kaushik, Amit, additional, Kala, Deepak, additional, Nagraik, Rupak, additional, Srivastava, Ashish, additional, Gupta, Shagun, additional, Singh, Bharat, additional, Kaushal, Ankur, additional, Walia, Yashika, additional, and Dhir, Sunny, additional

Published
2024
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8. Recent advances in ctDNA detection using electrochemical biosensor for cancer.

Author

Kumar, Sahil, Poria, Renu, Kala, Deepak, Nagraik, Rupak, Dhir, Yashika, Dhir, Sunny, Singh, Bharat, Kaushik, Naveen Kumar, Noorani, Md Salik, Kumar, Deepak, Gupta, Shagun, and Kaushal, Ankur

Subjects
CIRCULATING tumor DNA, TUMOR diagnosis, EARLY detection of cancer, POINT-of-care testing, CANCER diagnosis
Abstract

In the quest for early cancer diagnosis, early identification and treatment are paramount. Recently, ctDNA detection has emerged as a viable avenue for early screening of cancer. The examination of ctDNA in fluid biopsies has gained substantial attention in tumor diagnosis and therapy. Both the scientific community and industry are actively exploring this field. However, developing cost-effective, portable, and real-time ctDNA measurement methods using conventional gene detection equipment poses a significant challenge. This challenge has led to the exploration of alternative approaches. Electrochemical biosensors, distinguished by their heightened sensitivity, remarkable specificity, affordability, and excellent portability, have emerged as a promising avenue for ctDNA detection. This review is dedicated to the specific focus on ctDNA detection, highlighting recent advancements in this evolving detection technology. We aimed to reference previous studies related to ctDNA-targeted cancer detection using electrochemical biosensors to advocate the utilization of electrochemical biosensors in healthcare diagnostics. Further research is imperative for the effective integration of ctDNA analysis into point-of-care cancer testing. Innovative approaches utilizing multiple markers need to be explored to advance this technology and make substantial contributions to societal well-being. [ABSTRACT FROM AUTHOR]

Published
2024
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11. List of contributors

Author

Adhikari, Nav Raj, primary, Agrawal, Ganesh Kumar, additional, Ahmad, Niaz, additional, Ahmad, Rumana, additional, Araujo, Fabricio Almeida, additional, Aslam, Sidra, additional, Aydın, Yıldız, additional, Barh, Debmalya, additional, Benkeblia, Noureddine, additional, Benko-Iseppon, Ana Maria, additional, Bhardwaj, Ragini, additional, Binneck, Eliseu, additional, Bozhilova-Sakova, Milena Georgieva, additional, Braga, Marcus de Barros, additional, Buckseth, Tanuja, additional, Challam, Clarissa, additional, Choudhary, Ashish Kumar, additional, da Silva, Manassés, additional, da Silva, Wilson, additional, Das, Sauren, additional, De Los Santos, Edian Franklin Franco, additional, Dhakar, Kusum, additional, Dhir, Sunny, additional, Dhkal, Manmohan, additional, Dimitrova, Ivona Vassileva, additional, Dwivedi, Shruti, additional, Dwivedi, Upendra N., additional, Dwivedi, Vikas, additional, Eizenberg, Hanan, additional, Elias, Sabrina, additional, Ferreira-Neto, José Ribamar Costa, additional, Freilich, Shiri, additional, Gaikwad, Kishor, additional, Gaur, Rajarshi Kumar, additional, Gautam, Ranjana, additional, Ghosh, Sewali, additional, Gilles, Tijs, additional, Gupta, Archit, additional, Gupta, Kapil, additional, Gupta, Om Prakash, additional, Gupta, Ravi, additional, Gupta, Romasha, additional, Gupta, Supriya, additional, Haque, Taslima, additional, Hazra, Anjan, additional, Hossain, Md. Anowar, additional, Iyer, Niranjani, additional, Jaiswar, Akanksha, additional, Jasrotia, Rahul Singh, additional, Kaila, Tanvi, additional, Kamboj, Disha, additional, Kaur, Manjot, additional, Kaur, Parampreet, additional, Kaur, Ramandeep, additional, Khatri, Nisha, additional, Kido, Éderson Akio, additional, Kim, Sun Tae, additional, Kumar, Ashwani, additional, Kumar, Dileep, additional, Kumar, Manoj, additional, Kumar, Pradeep, additional, Kumar, Pravin, additional, Kumar, Satish, additional, Kumar, Shailesh, additional, Kushwaha, Ujjawal Kumar Singh, additional, Lucas, Stuart J., additional, Mahadani, Pradosh, additional, Malo, Richard, additional, Maurya, Suresh Kumar, additional, Meena, Nand Lal, additional, Meena, Vijay Singh, additional, Mehmood, Muhammad Aamer, additional, Minkina, Tatiana, additional, Mishra, Chandra Nath, additional, Mohideen, Habeeb Shaik, additional, Mor, Poonam, additional, Mukhopadhyay, Kunal, additional, Nanda, Satyabrata, additional, Narad, Priyanka, additional, Narayan, Aditya, additional, Natarajan, Nandakumar, additional, Negi, Chandranandani, additional, Nehra, Chitra, additional, Noor, Fatima, additional, Pal, Lalita, additional, Pandey, Veda P., additional, Petrov, Nikolay Manchev, additional, Poddar, Raju, additional, Prasad, Birendra, additional, Rahman, Mehboob-ur, additional, Rajput, Vishnu D., additional, Rakwal, Randeep, additional, Ramos, Rommel Thiago Jucá, additional, Rani, Asha, additional, Redhu, Neeru S., additional, Rishi, Narayan, additional, Riyazuddin, Riyazuddin, additional, Ronen, Zeev, additional, Sangeetha, Jeyabalan, additional, Sarkar, Abhijit, additional, Satapathy, Lopamudra, additional, Sengupta, Abhishek, additional, Seraj, Zeba, additional, Shahid, Saima, additional, Sharma, Pradeep, additional, Sharma, Priti, additional, Sharma, Swati, additional, Sheikh, Imran, additional, Shohan, Mohammad Umer Sharif, additional, Singh, Abhishek, additional, Singh, Ajeet, additional, Singh, Anil Kumar, additional, Singh, Anuradha, additional, Singh, Dharmendra, additional, Singh, Gyanendra Pratap, additional, Singh, Nisha, additional, Singh, Rajesh K., additional, Sonawala, Kevina, additional, Srivastava, Sudhanshu, additional, Stoyanova, Mariya Ivanova, additional, Tanveer, Aiman, additional, Thakur, Zoozeal, additional, Thangadurai, Devarajan, additional, Tiwari, Jagesh Kumar, additional, Tiwary, Basant K., additional, Torres, Renato Hidaka, additional, Tripathy, Sanjaya Shankar, additional, Tyagi, Aruna, additional, Ujinwal, Megha, additional, Uncuoğlu, Ahu Altınkut, additional, Verma, Rakesh Kumar, additional, Vyas, Pritesh, additional, Yadav, Ajit Kumar Singh, additional, Yadav, Anurag, additional, Yadav, Dinesh, additional, Yadav, Kanchan, additional, Yadav, Kusum, additional, Yadav, Manoj Kumar, additional, Yadav, Pramod Kumar, additional, Yashveer, Shikha, additional, Zarecki, Raphy, additional, and Şahin, Ezgi Çabuk, additional

Published
2022
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16. Vaccine development: Current trends and technologies

Author

Poria, Renu, primary, Kala, Deepak, additional, Nagraik, Rupak, additional, Dhir, Yashika, additional, Dhir, Sunny, additional, Singh, Bharat, additional, Kaushik, Naveen Kumar, additional, Noorani, Md Salik, additional, Kaushal, Ankur, additional, and Gupta, Shagun, additional

Published
2023
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25. Molecular characterization of sequence variants of Chrysanthemumchlorotic mottle viroidfrom Himachal Pradesh, India

Author

Walia, Yashika, Dhir, Sunny, and Hallan, Vipin

Abstract

Chrysanthemumis an important cut flower crop but is known to be infected with viruses and viroids. Here, we have identified and characterized two sequence variants of Chrysanthemum chlorotic mottle viroid(CCHMVd) from chrysanthemumvarieties Yellow Star and Orange Daisy that showed stunting, mottling and chlorosis-like symptoms, respectively. The viroid sequences were sequenced which upon BLAST analysis showed 97–98% sequence similarity to other CCHMVd isolates reported from India and world. CCHMVd disease incidence was found to be 15% that was higher in comparison to 8–10% in South Indian states. In phylogenetic analysis, the Palampur isolate grouped with other Indian isolates while isolate YS showed different ancestral origin and grouped separately with other world isolates.

Published
2022
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27. Molecular detection and characterization of Potato spindle tuber viroid(PSTVd) infecting Tomato (Solanum lycopersicumL.) in Karnataka State of India

Author

Shilpa, N., Dhir, Sunny, and Janardhana, G. R.

Abstract

Potato Spindle Tuber Viroid (PSTVd) is a non-coding, infectious, small, circular RNA known to cause disease in agricultural and horticultural plants. In the present work, an investigation was conducted in the southern districts of Karnataka state to assess the possible pospiviroid infections on tomato plants that are considered natural hosts for viroids. A total of 83 tomato samples showing disease symptoms (virus or viroid-like) along with healthy ones were collected and subjected to viroid detection by conventional Reverse Transcriptase Polymer Chain Reaction (RT-PCR) using universal (Pospi1-RE/Pospi1-FW) and a specific set of primers (3H1/2H1). The study confirmed the presence of PSTVd in one of the samples of tomato collected from Banghatta village of Mandy District, with an expected amplicon of ~ 361 bp. The bioassay conducted on tomato plants (cv. Rutgers) proved the association of PSTVd, which was further confirmed by RT-PCR. The amplicons were cloned, sequenced, and the representative sequences were deposited in the NCBI GenBank. The sequence alignment and secondary structure analysis of the isolated viroid with other reference sequences revealed the variations in the pathogenicity, central conserved region, and Terminal right domains. The variations observed between the isolated PSTVd with that of other Indian isolates support that viroid may have been transmitted among the crop plants, possibly through seed or mechanical means.

Published
2022
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30. Apple scar skin viroidnaked RNA is actively transmitted by the whitefly Trialeurodes vaporariorum

Author

Walia, Yashika, Dhir, Sunny, Zaidi, Aijaz Asghar, and Hallan, Vipin

Abstract

Nucleic acid transfer between plants is a phenomenon which is likely to occur in many ways in nature. We report here the active transmission of Apple scar skin viroid(ASSVd) naked ssRNA species by the whitefly Trialeurodes vaporariorum(Tv). Not only the viroid RNA, its DNA form was also identified from the insect. The viroid transfer efficiency was enhanced with the help of Cucumis sativusPhloem protein 2 (CsPP2), a plant protein known to translocate viroid RNAs. This PP2/ASSVd complex is stably present in the viroid infected cucumber plants, as was identified with the help of immunological reaction. As viroid-like secondary structures are found in some plant RNAs, and PP2 is known to bind and translocate several RNAs, the results have huge implications in transfer of these RNA species between plants visited by the whitefly.

Published
2015
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