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22 results on '"Dehouck, Lucie"'

2. ProNGF promotes brain metastasis through TrkA/EphA2 induced Src activation in triple negative breast cancer cells

Author

Cicero, Julien, Trouvilliez, Sarah, Palma, Martine, Ternier, Gaetan, Decoster, Laurine, Happernegg, Eloise, Barois, Nicolas, Van Outryve, Alexandre, Dehouck, Lucie, Bourette, Roland P., Adriaenssens, Eric, Lagadec, Chann, Tarhan, Cagatay Mehmet, Collard, Dominique, Souguir, Zied, Vandenhaute, Elodie, Maubon, Grégory, Sipieter, François, Borghi, Nicolas, Shimizu, Fumitaka, Kanda, Takashi, Giacobini, Paolo, Gosselet, Fabien, Maubon, Nathalie, Le Bourhis, Xuefen, Van Seuningen, Isabelle, Mysiorek, Caroline, and Toillon, Robert-Alain

Published
2023
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6. Quantitative Targeted Absolute Proteomics for Better Characterization of an In Vitro Human Blood–Brain Barrier Model Derived from Hematopoietic Stem Cells

Author

Dehouck, Marie-Pierre, primary, Tachikawa, Masanori, additional, Hoshi, Yutaro, additional, Omori, Kotaro, additional, Maurage, Claude-Alain, additional, Strecker, Guillaume, additional, Dehouck, Lucie, additional, Boucau, Marie-Christine, additional, Uchida, Yasuo, additional, Gosselet, Fabien, additional, Terasaki, Tetsuya, additional, and Karamanos, Yannis, additional

Published
2022
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7. Interaction of enterovirus A71 with the human blood-brain barrier: an in vitro study

Author

Gaume, Léa, Chabrolles, Hélène, Simon, Isabelle, Dehouck, Lucie, Gosselet, Fabien, Mirand, Audrey, Archimbaud, Christine, Bailly, Jean-Luc, BRICHEUX, Genevieve, Epidémiologie et pathogénie des infections à entérovirus (EPIE), CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-Laboratoire Microorganismes : Génome et Environnement (LMGE), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Laboratoire de la Barrière Hémato-Encéphalique (LBHE), and Université d'Artois (UA)

Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BC] Life Sciences [q-bio]/Cellular Biology
Abstract

International audience

Published
2022

8. Neurotrophins promotes brain metastasis of triple negative breast cancer through Src kinase family activation

Author

Cicero, Julien, Trouvilliez, Sarah, Palma, Martine, Ternier, Gaetan, Decoster, Laurine, Barrois, Nicolas, Dehouck, Lucie, Happernegg, Eloise, Bourette, Roland, Adriaenssens, Eric, Lagadec, Chann, Tarhan, Mehmet, Collard, Dominique, Souguir, Zied, Vandenhaute, Elodie, Maubon, Grégory, Maubon, Nathalie, SIPIETER, FRANCOIS, Sipieter, François, Borghi, Nicolas, Giacobini, Paolo, Prevost, Vincent, Gosselet, Fabien, Le Bourhis, Xuefen, van Seuningen, Isabelle, Mysiorek, Caroline, Toillon, Robert-Alain, IEMN, Collection, Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 (CANTHER), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] (UNICANCER/Lille), Université de Lille-UNICANCER, CHU Lille, Lille Neurosciences & Cognition - U 1172 (LilNCog), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Plateformes Lilloises en Biologie et Santé - UAR 2014 - US 41 (PLBS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Bio-Micro-Electro-Mechanical Systems - IEMN (BIOMEMS - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), JUNIA (JUNIA), Université catholique de Lille (UCL), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), HCS-Pharma Loos [Lille] (HCS-PL), Institut Jacques Monod (IJM (UMR_7592)), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and Institut National de la Santé et de la Recherche Médicale (INSERM)

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics]
Abstract

ORAL; International audience; With nearly 2.3 million cases diagnosed worldwide each year and an estimated 685 000 deaths by 2020, breast cancer is the leading cause of cancer-related death in women. Brain metastases cause severe cognitive complications that severely impair quality of life. In TN breast cancer, the prognosis for brain metastases is particularly poor with a median survival of no more than 6 months. It is therefore crucial to know the molecular actors that promote the metastatic dissemination of triple negative breast cancer to the brain but also to prevent the proliferation of brain micrometastases that can cause fatal recurrences. In order to recapitulate several final stages of brain metastasis, we used both human Blood- Brain-Barrier in vitro model, human organotypic 3D extracellular in vitro matrix, mice brain slices organotypic ex vivo culture and in vivo mice xenograft. These models are coupled with single cell, 3D and real time imaging techniques, including FRET biosensing. This unique experimental approach allows us to study the involvement of signaling pathways in these different biological processes. Using this innovative method, we have identified that inhibition neurotrophins receptor leads to a decrease in the activity of the underlying signaling pathways and consequently to a decrease in the ability of breast cancer cells to pass through the BBB, to grow and to colonize the brain parenchyma.

Published
2022

9. A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Author

Rizzi, Eleonora, primary, Deligne, Clémence, primary, Dehouck, Lucie, primary, Bilardo, Roberta, primary, Sano, Yasutero, primary, Shimizu, Fumitaka, primary, Kanda, Takashi, primary, Resmini, Marina, primary, Gosselet, Fabien, primary, Dehouck, Marie-Pierre, primary, and Mysiorek, Caroline, primary

Published
2021
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11. Time-Dependent Internalization of Polymer-Coated Silica Nanoparticles in Brain Endothelial Cells and Morphological and Functional Effects on the Blood-Brain Barrier

Author

Bittner, Aniela, Gosselet, Fabien, Sevin, Emmanuel, Dehouck, Lucie, Ducray, Angélique D., Gaschen, Véronique, Stoffel, Michael H., Hansang, Cho, Mevissen, Meike, University of Bern, Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), and Sungkyunkwan University [Suwon] (SKKU)

Subjects
615 Pharmacology & therapeutics, prescription drugs, 3D model, Cell Survival, Polymers, [SDV]Life Sciences [q-bio], education, transendothelial electrical resistance, 610 Medicine & health, Lymphocyte Activation, Article, lcsh:Chemistry, Animals, Humans, lcsh:QH301-705.5, Cells, Cultured, B-Lymphocytes, Cerebral Revascularization, NF-kappa B, Brain, Endothelial Cells, Biological Transport, 500 Science, Silicon Dioxide, co-culture, lcsh:Biology (General), lcsh:QD1-999, Blood-Brain Barrier, 570 Life sciences, biology, Nanoparticles, Cattle, Laser Therapy, permeability, Pericytes
Abstract

International audience; Nanoparticle (NP)-assisted procedures including laser tissue soldering (LTS) offer advantages compared to conventional microsuturing, especially in the brain. In this study, effects of polymer-coated silica NPs used in LTS were investigated in human brain endothelial cells (ECs) and blood-brain barrier models. In the co-culture setting with ECs and pericytes, only the cell type directly exposed to NPs displayed a time-dependent internalization. No transfer of NPs between the two cell types was observed. Cell viability was decreased relatively to NP exposure duration and concentration. Protein expression of the nuclear factor ĸ-light-chain-enhancer of activated B cells and various endothelial adhesion molecules indicated no initiation of inflammation or activation of ECs after NP exposure. Differentiation of CD34+ ECs into brain-like ECs co-cultured with pericytes, blood-brain barrier (BBB) characteristics were obtained. The established endothelial layer reduced the passage of integrity tracer molecules. NP exposure did not result in alterations of junctional proteins, BBB formation or its integrity. In a 3-dimensional setup with an endothelial tube formation and tight junctions, barrier formation was not disrupted by the NPs and NPs do not seem to cross the blood-brain barrier. Our findings suggest that these polymer-coated silica NPs do not damage the BBB

Published
2021

12. Additional file 1 of Contribution of brain pericytes in blood–brain barrier formation and maintenance: a transcriptomic study of cocultured human endothelial cells derived from hematopoietic stem cells

Author

Heymans, Marjolein, Figueiredo, Ricardo, Dehouck, Lucie, Francisco, David, Yasuteru Sano, Fumitaka Shimizu, Kanda, Takashi, Bruggmann, Rémy, Engelhardt, Britta, Winter, Peter, Gosselet, Fabien, and Culot, Maxime

Abstract

Additional file 1. Supplementary table depicting sequencing statistics. Number of raw reads sequenced and number of unique reads after removal of duplicates generated during polymerase chain reaction.

Published
2020
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13. Zika Virus Infection Promotes Local Inflammation, Cell Adhesion Molecule Upregulation, and Leukocyte Recruitment at the Blood-Brain Barrier

Author

Clé, Marion, primary, Desmetz, Caroline, additional, Barthelemy, Jonathan, additional, Martin, Marie-France, additional, Constant, Orianne, additional, Maarifi, Ghizlane, additional, Foulongne, Vincent, additional, Bolloré, Karine, additional, Glasson, Yaël, additional, De Bock, Frédéric, additional, Blaquiere, Marine, additional, Dehouck, Lucie, additional, Pirot, Nelly, additional, Tuaillon, Edouard, additional, Nisole, Sébastien, additional, Najioullah, Fatiha, additional, Van de Perre, Philippe, additional, Cabié, André, additional, Marchi, Nicola, additional, Gosselet, Fabien, additional, Simonin, Yannick, additional, and Salinas, Sara, additional

Published
2020
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16. Transient blood–brain barrier disruption is induced by low pulsed electrical fields in vitro: an analysis of permeability and trans-endothelial electric resistivity

Author

Sharabi, Shirley, primary, Bresler, Yael, additional, Ravid, Orly, additional, Shemesh, Chen, additional, Atrakchi, Dana, additional, Schnaider-Beeri, Michal, additional, Gosselet, Fabien, additional, Dehouck, Lucie, additional, Last, David, additional, Guez, David, additional, Daniels, Dianne, additional, Mardor, Yael, additional, and Cooper, Itzik, additional

Published
2019
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17. A silicon nanomembrane platform for the visualization of immune cell trafficking across the human blood–brain barrier under flow

Author

Mossu, Adrien, primary, Rosito, Maria, additional, Khire, Tejas, additional, Li Chung, Hung, additional, Nishihara, Hideaki, additional, Gruber, Isabelle, additional, Luke, Emma, additional, Dehouck, Lucie, additional, Sallusto, Federica, additional, Gosselet, Fabien, additional, McGrath, James L, additional, and Engelhardt, Britta, additional

Published
2018
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18. Blood-Brain Barrier Cellular Responses Toward Organophosphates: Natural Compensatory Processes and Exogenous Interventions to Rescue Barrier Properties

Author

Ravid, Orly, primary, Elhaik Goldman, Shirin, additional, Macheto, David, additional, Bresler, Yael, additional, De Oliveira, Raquel Ines, additional, Liraz-Zaltsman, Sigal, additional, Gosselet, Fabien, additional, Dehouck, Lucie, additional, Beeri, Michal Schnaider, additional, and Cooper, Itzik, additional

Published
2018
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21. Enterovirus A71 crosses a human blood-brain barrier model through infected immune cells.

Author

Gaume L, Chabrolles H, Bisseux M, Lopez-Coqueiro I, Dehouck L, Mirand A, Henquell C, Gosselet F, Archimbaud C, and Bailly J-L

Subjects
Humans, Virus Replication, Monocytes virology, Monocytes immunology, Pericytes virology, Leukocytes virology, Leukocytes immunology, Brain virology, Killer Cells, Natural immunology, Neutrophils immunology, Neutrophils virology, Blood-Brain Barrier virology, Enterovirus A, Human genetics, Enterovirus A, Human physiology, Enterovirus Infections virology, Enterovirus Infections immunology, Endothelial Cells virology
Abstract

Enterovirus A71 (EV-A71) is associated with neurological conditions such as acute meningitis and encephalitis. The virus is detected in the bloodstream, and high blood viral loads are associated with central nervous system (CNS) manifestations. We used an in vitro blood-brain barrier (BBB) model made up of human brain-like endothelial cells (hBLECs) and brain pericytes grown in transwell systems to investigate whether three genetically distinct EV-A71 strains (subgenogroups C1, C1-like, and C4) can cross the human BBB. EV-A71 poorly replicated in hBLECs, which released moderate amounts of infectious viruses from their luminal side and trace amounts of infectious viruses from their basolateral side. The barrier properties of hBLECs were not impaired by EV-A71 infection. We investigated the passage through hBLECs of EV-A71-infected white blood cells. EV-A71 strains efficiently replicated in immune cells, including monocytes, neutrophils, and NK/T cells. Attachment to hBLECs of immune cells infected with the C1-like virus was higher than attachment of cells infected with C1-06. EV-A71 infection did not impair the transmigration of immune cells through hBLECs. Overall, EV-A71 targets different white blood cell populations that have the potential to be used as a Trojan horse to cross hBLECs more efficiently than cell-free EV-A71 particles.IMPORTANCEEnterovirus A71 (EV-A71) was first reported in the USA, and numerous outbreaks have since occurred in Asia and Europe. EV-A71 re-emerged as a new multirecombinant strain in 2015 in Europe and is now widespread. The virus causes hand-foot-and-mouth disease in young children and is involved in nervous system infections. How the virus spreads to the nervous system is unclear. We investigated whether white blood cells could be infected by EV-A71 and transmit it across human endothelial cells mimicking the blood-brain barrier protecting the brain from adverse effects. We found that endothelial cells provide a strong roadblock to prevent the passage of free virus particles but allow the migration of infected immune cells, including monocytes, neutrophils, and NK/T cells. Our data are consistent with the potential role of immune cells in the pathogenesis of EV-A71 infections by spreading the virus in the blood and across the human blood-brain barrier., Competing Interests: The authors declare no conflict of interest.

Published
2024
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22. An In Vitro Human Blood-Brain Barrier Model to Study Breast Cancer Brain Metastasis.

Author

Mysiorek C, Dehouck L, Gosselet F, and Dehouck MP

Subjects
Biological Transport, Blood-Brain Barrier, Brain pathology, Endothelial Cells, Female, Humans, Brain Neoplasms genetics, Breast Neoplasms pathology
Abstract

Studying the mechanisms of breast cancer cells in brain metastases is challenging, considering the high specificity of the blood-brain barrier (BBB) with whom breast cancer cells have to interact and cross in order to reach the brain parenchyma. While numerous in vitro BBB models are available, the setting of the model and phenotype of the endothelial cells (ECs) of the BBB model are essential to obtain relevant results.In this chapter, we describe a method to establish a human in vitro BBB model to study adhesion of breast cancer cells and the adaptation of the method for trans-endothelial migration assay keeping the appropriate BBB phenotype of the ECs., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)

Published
2022
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