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Your search keyword '"Damle-Vartak, Amruta"' showing total 14 results
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14 results on '"Damle-Vartak, Amruta"'

1. Dynamic YAP expression in the non-parenchymal liver cell compartment controls heterologous cell communication

Author

Liu, Kaijing, primary, Wehling, Lilija, additional, Wan, Shan, additional, Weiler, Sofia M. E., additional, Tóth, Marcell, additional, Ibberson, David, additional, Marhenke, Silke, additional, Ali, Adnan, additional, Lam, Macrina, additional, Guo, Te, additional, Pinna, Federico, additional, Pedrini, Fabiola, additional, Damle-Vartak, Amruta, additional, Dropmann, Anne, additional, Rose, Fabian, additional, Colucci, Silvia, additional, Cheng, Wenxiang, additional, Bissinger, Michaela, additional, Schmitt, Jennifer, additional, Birner, Patrizia, additional, Poth, Tanja, additional, Angel, Peter, additional, Dooley, Steven, additional, Muckenthaler, Martina U., additional, Longerich, Thomas, additional, Vogel, Arndt, additional, Heikenwälder, Mathias, additional, Schirmacher, Peter, additional, and Breuhahn, Kai, additional

Published
2024
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4. Biliary epithelial cell-specific RAGE controls ductular reaction-mediated fibrosis during cholestasis

Author

Macrina Lam, Wai Ling, primary, Damle-Vartak, Amruta, additional, Gabernet, Gisela, additional, Schneller, Doris, additional, Poth, Tanja, additional, Jörs, Simone, additional, Sator-Schmitt, Melanie, additional, De Ponti, Aurora, additional, Weiß, Lena, additional, Heikenwälder, Mathias, additional, Vartak, Nachiket, additional, Hengstler, Jan G., additional, Geisler, Fabian, additional, Nahnsen, Sven, additional, and Angel, Peter, additional

Published
2022
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5. The hepatocyte export carrier inhibition assay improves the separation of hepatotoxic from non-hepatotoxic compounds

Author

Brecklinghaus, Tim, primary, Albrecht, Wiebke, additional, Kappenberg, Franziska, additional, Duda, Julia, additional, Vartak, Nachiket, additional, Edlund, Karolina, additional, Marchan, Rosemarie, additional, Ghallab, Ahmed, additional, Cadenas, Cristina, additional, Günther, Georgia, additional, Leist, Marcel, additional, Zhang, Mian, additional, Gardner, Iain, additional, Reinders, Jörg, additional, Russel, Frans GM., additional, Foster, Alison J., additional, Williams, Dominic P., additional, Damle-Vartak, Amruta, additional, Grandits, Melanie, additional, Ecker, Gerhard, additional, Kittana, Naim, additional, Rahnenführer, Jörg, additional, and Hengstler, Jan G., additional

Published
2022
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7. Intravital Dynamic and Correlative Imaging of Mouse Livers Reveals Diffusion-Dominated Canalicular and Flow-Augmented Ductular Bile Flux

Author

Vartak, Nachiket, Guenther, Georgia, Joly, Florian, Damle-Vartak, Amruta, Wibbelt, Gudrun, Fickel, Joerns, Joers, Simone, Begher-Tibbe, Brigitte, Friebel, Adrian, Wansing, Kasimir, Ghallab, Ahmed, Rosselin, Marie, Boissier, Noemie, Vignon-Clementel, Irene, Hedberg, Christian, Geisler, Fabian, Hofer, Heribert, Jansen, Peter, Hoehme, Stefan, Drasdo, Dirk, Hengstler, Jan G., Vartak, Nachiket, Guenther, Georgia, Joly, Florian, Damle-Vartak, Amruta, Wibbelt, Gudrun, Fickel, Joerns, Joers, Simone, Begher-Tibbe, Brigitte, Friebel, Adrian, Wansing, Kasimir, Ghallab, Ahmed, Rosselin, Marie, Boissier, Noemie, Vignon-Clementel, Irene, Hedberg, Christian, Geisler, Fabian, Hofer, Heribert, Jansen, Peter, Hoehme, Stefan, Drasdo, Dirk, and Hengstler, Jan G.

Abstract

Background and Aims: Small-molecule flux in tissue microdomains is essential for organ function, but knowledge of this process is scant due to the lack of suitable methods. We developed two independent techniques that allow the quantification of advection (flow) and diffusion in individual bile canaliculi and in interlobular bile ducts of intact livers in living mice, namely fluorescence loss after photoactivation and intravital arbitrary region image correlation spectroscopy. Approach and Results: The results challenge the prevailing "mechano-osmotic" theory of canalicular bile flow. After active transport across hepatocyte membranes, bile acids are transported in the canaliculi primarily by diffusion. Only in the interlobular ducts is diffusion augmented by regulatable advection. Photoactivation of fluorescein bis-(5-carboxymethoxy-2-nitrobenzyl)-ether in entire lobules demonstrated the establishment of diffusive gradients in the bile canalicular network and the sink function of interlobular ducts. In contrast to the bile canalicular network, vectorial transport was detected and quantified in the mesh of interlobular bile ducts. Conclusions: The liver consists of a diffusion-dominated canalicular domain, where hepatocytes secrete small molecules and generate a concentration gradient and a flow-augmented ductular domain, where regulated water influx creates unidirectional advection that augments the diffusive flux.

Published
2021
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8. Intravital Dynamic and Correlative Imaging of Mouse Livers Reveals Diffusion‐Dominated Canalicular and Flow‐Augmented Ductular Bile Flux

Author

Vartak, Nachiket, primary, Guenther, Georgia, additional, Joly, Florian, additional, Damle‐Vartak, Amruta, additional, Wibbelt, Gudrun, additional, Fickel, Jörns, additional, Jörs, Simone, additional, Begher‐Tibbe, Brigitte, additional, Friebel, Adrian, additional, Wansing, Kasimir, additional, Ghallab, Ahmed, additional, Rosselin, Marie, additional, Boissier, Noemie, additional, Vignon‐Clementel, Irene, additional, Hedberg, Christian, additional, Geisler, Fabian, additional, Hofer, Heribert, additional, Jansen, Peter, additional, Hoehme, Stefan, additional, Drasdo, Dirk, additional, and Hengstler, Jan G., additional

Published
2021
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9. Intravital dynamic and correlative imaging reveals diffusion‐dominated canalicular and flow‐augmented ductular bile flux

Author

Vartak, Nachiket, Guenther, Georgia, Joly, Florian, Damle-Vartak, Amruta, Wibbelt, Gudrun, Fickel, Jörns, Jörs, Simone, Begher-Tibbe, Brigitte, Friebel, Adrian, Wansing, Kasimir, Ghallab, Ahmed, Rosselin, Marie, Boissier, Noemie, Vignon-Clementel, Irene, Hedberg, Christian, Geisler, Fabian, Hofer, Heribert, Jansen, Peter, Hoehme, Stefan, Drasdo, Dirk, Hengstler, Jan, Leibniz Research Centre for Working Environment and Human Factors [Dortmund] (IFADO), Technische Universität Dortmund [Dortmund] (TU), Modelling and Analysis for Medical and Biological Applications (MAMBA), Inria de Paris, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire Jacques-Louis Lions (LJLL (UMR_7598)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Leibniz Institute for Zoo and Wildlife Research (IZW), Leibniz Association, University of Potsdam, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Institut für Informatik [Leipzig], Universität Leipzig [Leipzig], South Valley University [Qena], Umeå University, Numerical simulation of biological flows (REO), Sorbonne Université (SU)-Inria de Paris, Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Freie Universität Berlin, University of Amsterdam [Amsterdam] (UvA), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), University of Potsdam = Universität Potsdam, Universität Leipzig, SImulations en Médecine, BIOtechnologie et ToXicologie de systèmes multicellulaires (SIMBIOTX ), Inria Saclay - Ile de France, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), and ANR-16-RHUS-0005,iLite,iLite(2016)

Subjects
[SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Bile flux proceeds by diffusion in canaliculi, Augmented by advection in ducts, digestive system
Abstract

International audience; Small-molecule flux in tissue-microdomains is essential for organ function, but knowledge of this process is scant due to the lack of suitable methods. We developed two independent techniques that allow the quantification of advection (flow) and diffusion in individual bile canaliculi and in interlobular bile ducts of intact livers in living mice, namely Fluorescence Loss After Photoactivation (FLAP) and Intravital Arbitrary Region Image Correlation Spectroscopy (IVARICS). The results challenge the prevailing 'mechano-osmotic' theory of canalicular bile flow. After active transport across hepatocyte membranes bile acids are transported in the canaliculi primarily by diffusion. Only in the interlobular ducts, diffusion is augmented by regulatable advection. Photoactivation of fluorescein bis-(5-carboxymethoxy-2-nitrobenzyl)-ether (CMNB-caged fluorescein) in entire lobules demonstrated the establishment of diffusive gradients in the bile canalicular network and the sink function of interlobular ducts. In contrast to the bile canalicular network, vectorial transport was detected and quantified in the mesh of interlobular bile ducts. In conclusion, the liver consists of a diffusion dominated canalicular domain, where hepatocytes secrete small molecules and generate a concentration gradient and a flow-augmented ductular domain, where regulated water influx creates unidirectional advection that augments the diffusive flux.

Published
2020

10. Intravital multi-modal flux analysis reveals the transport mechanism of bile acids through hepatic microconduits

Author

Vartak, Nachiket, Guenther, Georgia, Joly, Florian, Damle-Vartak, Amruta, Wibbelt, Gudrun, Fickel, Jörns, Jörs, Simone, Begher-Tibbe, Brigitte, Friebel, Adrian, Wansing, Kasimir, Ghallab, Ahmed, Rosselin, Marie, Boissier, Noemie, Vignon-Clementel, Irene, Hedberg, Christian, Geisler, Fabian, Hofer, Heribert, Jansen, Peter, Hoehme, Stefan, Drasdo, Dirk, and Hengstler, Jan G.

Subjects
digestive system
Abstract

Small-molecule flux in tissue-microdomains is essential for organ function, but knowledge of this process is scant due to the lack of suitable methods applicable to live animals. We developed a methodology based on dynamic and correlative imaging for quantitative intravital flux analysis. Application to the liver, challenged the prevailing ‘mechano-osmotic’ theory of canalicular bile flow. After active transport across hepatocyte membranes bile salts are transported in the canaliculi primarily by diffusion. Only in the interlobular ducts, diffusion is augmented by regulatable advection. We corroborate these observations with in silico simulations and pan-species comparisons of lobule size. This study demonstrates a flux mechanism, where the energy invested in transmembrane transport entropically dissipates in a sub-micron scale vessel network. One Sentence Summary Bile flux proceeds by diffusion in canaliculi, augmented by advection in ducts.

Published
2019
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12. Intravital dynamic and correlative imaging reveals diffusion-dominated canalicular and flow-augmented ductular bile flux

Author

Vartak, Nachiket, primary, Guenther, Georgia, additional, Joly, Florian, additional, Damle-Vartak, Amruta, additional, Wibbelt, Gudrun, additional, Fickel, Jörns, additional, Jörs, Simone, additional, Begher-Tibbe, Brigitte, additional, Friebel, Adrian, additional, Wansing, Kasimir, additional, Ghallab, Ahmed, additional, Rosselin, Marie, additional, Boissier, Noemie, additional, Vignon-Clementel, Irene, additional, Hedberg, Christian, additional, Geisler, Fabian, additional, Hofer, Heribert, additional, Jansen, Peter, additional, Hoehme, Stefan, additional, Drasdo, Dirk, additional, and Hengstler, Jan G., additional

Published
2019
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13. Single cell polarity in liquid phase facilitates tumour metastasis

Author

Lorentzen, Anna, primary, Becker, Paul F., additional, Kosla, Jan, additional, Saini, Massimo, additional, Weidele, Kathrin, additional, Ronchi, Paolo, additional, Klein, Corinna, additional, Wolf, Monika J., additional, Geist, Felix, additional, Seubert, Bastian, additional, Ringelhan, Marc, additional, Mihic-Probst, Daniela, additional, Esser, Knud, additional, Roblek, Marko, additional, Kuehne, Felix, additional, Bianco, Gaia, additional, O’Connor, Tracy, additional, Müller, Quentin, additional, Schuck, Kathleen, additional, Lange, Sebastian, additional, Hartmann, Daniel, additional, Spaich, Saskia, additional, Groß, Olaf, additional, Utikal, Jochen, additional, Haferkamp, Sebastian, additional, Sprick, Martin R., additional, Damle-Vartak, Amruta, additional, Hapfelmeier, Alexander, additional, Hüser, Norbert, additional, Protzer, Ulrike, additional, Trumpp, Andreas, additional, Saur, Dieter, additional, Vartak, Nachiket, additional, Klein, Christoph A., additional, Polzer, Bernhard, additional, Borsig, Lubor, additional, and Heikenwalder, Mathias, additional

Published
2018
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14. Pipe-3D: A Pipeline Based on Immunofluorescence, 3D Confocal Imaging, Reconstructions, and Morphometry for Biliary Network Analysis in Cholestasis.

Author

Damle-Vartak A, Begher-Tibbe B, Gunther G, Geisler F, Vartak N, and Hengstler JG

Subjects
Alanine Transaminase metabolism, Aspartate Aminotransferases metabolism, Microscopy, Confocal, Bile Ducts metabolism, Cholestasis metabolism, Fluorescent Antibody Technique methods
Abstract

Cholestasis, the impairment of bile flux out of the liver, is a common complication of many pathological liver disorders, such as cholangiopathies, primary biliary sclerosis, and primary biliary cirrhosis. Besides accumulation of bile acids in the liver and blood, it leads to a proliferative response of the biliary tree termed as a ductular reaction. The ductular reaction is characterized by enhanced proliferation of cholangiocytes, which form the epithelial lining of bile ducts. This strong reaction of the biliary tree has been reported to generate a source of progenitor cells that can differentiate to hepatocytes or cholangiocytes during regeneration. On the other hand, it can cause periportal fibrosis eventually progressing to cirrhosis and death. In 2D histology, this leads to the appearance of an increased number of duct lumina per area of tissue. Yet, the biliary tree is a 3D vstructure and the appearance of lumina in thin slices may be explained by the appearance of novel ducts or by ramification or convolution of existing ducts in 3D. In many such aspects, traditional 2D histology on thin slices limits our understanding of the response of the biliary tree. A comprehensive understanding of architecture remodeling of the biliary network in cholestasis depends on robust 3D sample preparation and analysis methods. To that end, we describe pipe-3D, a processing and analysis pipeline visualization based on immunofluorescence, confocal imaging, surface reconstructions, and automated morphometry of the biliary network in 3D at subcellular resolution. This pipeline has been used to discover extensive remodeling of interlobular bile ducts in cholestasis, wherein elongation, branching, and looping create a dense ductular mesh around the portal vein branch. Surface reconstructions generated by Pipe-3D from confocal data also show an approximately fivefold enhancement of the luminal duct surface through corrugation of the epithelial lamina, which may increase bile reabsorption and alleviate cholestasis. The response of interlobular ducts in cholestasis was shown to be in sharp contrast to that of large bile ducts, de novo duct formation during embryogenesis. It is also distinct from ductular response in other models of hepatic injury such as choline-deficient, ethionine-supplemented diet, where parenchymal tissue invasion by ducts and their branches is observed. Pipe-3D is applicable to any model of liver injury, and optionally integrates tissue clearing techniques for 3D analysis of thick (>500 μm) tissue sections.

Published
2019
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