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Made available in DSpace on 2021-06-25T11:24:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-27 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Fundo de Defesa da Citricultura-Fundecitrus Embrapa Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Huanglongbing (HLB), the most important citrus disease worldwide, is associated with bacteria transmitted by the Asian citrus psyllid (ACP) preferably through new shoots present in the canopy. In a commercial citrus plant, the vegetative growth of the scion is influenced by the rootstock variety in which it is grafted. Although all commercial citrus varieties planted in recent years are susceptible to HLB, the dynamics of the rootstock in grafted plant could influence the progress of HLB, whether at the plant or grove scale. In this work, HLB incidence in 'Valencia' sweet orange grafted onto 16 rootstocks and its relationship to the tree canopy volume and flushing dynamics were evaluated in a field trial under ACP control. The experiment was conducted under rainfed conditions in Bebedouro, state of Sao Paulo, Brazil, from 2011 to 2019. 'Flying Dragon' trifoliate orange known for its dwarfing characteristics was used as the rootstock. A reduction in canopy volume by 77% at 8 years of age were observed compared to the most vigorous rootstocks. The frequency of flush shoots of 'Valencia' sweet orange was not influenced by the rootstock, but the abundance of flush shoots was lower on three semi-dwarfing rootstocks and as well as 'Flying Dragon'. Although HLB incidence on 'Flying Dragon' was lower than on 'Rangpur' lime and other three semi-standard rootstocks (trees with canopy volume between 51 and 75% of the 'Rangpur' lime canopy volume), all other combinations had similar HLB disease progress regardless of the canopy volume and flushing dynamics. Moreover, under field conditions, variations on the cumulative HLB incidence greater than 26% were necessary to significantly separate rootstocks. Therefore, the results suggest that true dwarfing rootstocks have potential to integrate the management program for HLB and that mechanisms in addition to tree vigor appear to be involved in the host-vector relationship. Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil Embrapa Mandioca & Fruticultura, BR-44380000 Cruz Das Almas, BA, Brazil Fundecitrus, BR-14807040 Araraquara, SP, Brazil Univ Estadual Paulista, Fac Ciencias Agr & Vet, BR-14884900 Jaboticabal, SP, Brazil FAPESP: 2014/26758-0 Embrapa: MP 02.13.03.003.00.01 Embrapa: 22.16.04.030.00.00

Purpose Porous structures have been widely used in filtration, medical implants and aerospace field. In the filtration field, the study of permeability of the porous structures is of great importance. This paper aims to study the influence of selective laser sintering/melting (SLS/SLM) process parameters on porosity and permeability of stainless steels filters with gradient porosity (FGM). Design/methodology/approach AISI 316L stainless steel powder was used to manufacture FGM filters by varying the hatch distance while other process parameters were fixed. The relationship between porosity and permeability of such FGM filters was investigated by means of Archimedes’ and Forchheimer’s laws. The gradient of porosity was also analyzed by means of micro-computed tomography. Findings The results have confirmed the ability of SLS/SLM in controlling porosity of the final product by varying the hatch distance. Further, the results allow to assume that FGM filters will have particle retention capacity related to lowest porosity value (which in turn is associated with the lowest hatch distance value used), while it will work at lower pressure drops – or at higher flow rates for equal pressure drop – when compared to a filter without gradient porosity. Originality/value Some research found in recent literature has showed the relationship between SLS/SLM process parameters and permeability of stainless steel porous structures. However, this paper fulfils the need to understand the relationship between SLS/SLM process parameters, porosity and permeability behavior of functionally graded porous structures (FGM filters).

The isotropic Stoner-Wohlfarth model can be applied for the determination of the anisotropy field in powders. Samples of strontium ferrite - SrFe12O19 – were measured in Vibrating Sample Magnetometer (VSM). By means of the fitting of the first quadrant of the hysteresis curve the anisotropy field was estimated as 19-20 kOe for the SrFe12O19 phase. The main advantage of the method is its simplicity, because compacting or sintering of the powder samples is not necessary

Acknowledgments: TCS and RPP acknowledge RL3-TECT-NORTE-01-0124-FEDER-000020, co-financed by ON.2-O Novo Norte; and FCT grants: SFRH/BPD/101952/2014 and SFRH/BPD/101886/2014, respectively. LPS acknowledges FCT grant SFRH/BD/78025/2011.

The advances achieved by cell-based therapies to treat autoimmune diseases such as rheumatoid arthritis (RA) and multiple sclerosis (MS), despite promising, are still insufficient for the current demands. RA and MS therapeutic approaches follow world guidelines to use disease modifying drugs and biological agents that, regardless of some good results in clinical outcomes, are well known for several systemic secondary side effects. Dendrimers are custom-made nanoparticles with proved clinical potential, displaying proper size, chemistries, immunomodulatory, and anti-inflammatory properties. This has directed their potential use as drug delivery systems for cancer therapy, for instance. This review manuscript discloses the hidden potential behind dendrimers as alternative viable solutions to treat RA and MS, by focusing in the most recent reports describing the use of dendrimers for suppressing inflammation and possibly preventing disease progression. The advantages of their use as compared with current applied therapies is also discussed herein., This work was developed under the scope of the Project NORTE‐08‐ 5369‐FSE‐000044 to which the author Daniel B. Rodrigues acknowledges for his PhD grant. The author Tírcia C. Santos acknowledges to the Fundação para a Ciência e Tecnologia (FCT) for her postdoc grant (SFRH/BPD/101952/2014). The FCT distinction attributed to Joaquim M. Oliveira under the Investigador FCT program (IF/01285/ 2015) is also greatly acknowledged., info:eu-repo/semantics/publishedVersion

Extracellular calcium (Ca 2 + o ) and its receptor, the Ca 2 + -sensing receptor (CaSR), play an important role in prostate physiology, and it has been shown that the deregulation of Ca 2 + homeostasis and the overexpression of CaSR are involved in prostate cancer (PCa). Regucalcin (RGN), a Ca 2 + -binding protein that plays a relevant role in intracellular Ca 2 + homeostasis, was identified as an under-expressed protein in human PCa. Moreover, RGN was associated with suppression of cell proliferation, suggesting that the loss of RGN may favor development and progression of PCa. This work aims to unveil the role of Ca 2 + o on RGN expression and viability of non-neoplastic (PNT1A) and neoplastic (LNCaP) prostate cell lines. It was demonstrated that Ca 2 + o up-regulates RGN expression in both cell lines, but important differences were found between cells for dose- and time-responses to Ca 2 + o treatment. It was also shown that high [Ca 2 + ] o triggers different effects on cell proliferation of neoplastic and non-neoplastic PCa cells, which seems to be related with RGN expression levels. This suggests the involvement of RGN in the regulation of cell proliferation in response to Ca 2 + o treatment. Also, the effect of Ca 2 + o on CaSR expression seems to be dependent of RGN expression, which is strengthened by the fact that RGN-knockdown in PNT1A cells increases the CaSR expression, whereas transgenic rats overexpressing RGN exhibit low levels of CaSR. Overall, our results highlighted the importance of RGN as a regulatory protein in Ca 2 + -dependent signaling pathways and its deregulation of RGN expression by Ca 2 + o may contribute for onset and progression of PCa.

RESUMO – O cultivo de microalga em meios heterotroficos e uma alternativa promissora para obtencao de pigmentos como o β-caroteno. Assim, o objetivo do trabalho foi a quantificacao deste pigmento em larga escala a partir da biomassa de microalga Phormidium sp. A producao desse pigmento foi conduzida atraves do cultivo heterotrofico, utilizando agua residuaria como meio. A extracao do β-caroteno foi obtido por ultra-som (Sonics, Anaheim, CA, EUA) com sonda de 13 mm de diâmetro. Os isomeros foram quantificados de acordo com os seguintes parâmetros: ordem de eluicao na coluna C30 de HPLC, cromatografia com padroes autenticos, e espectros de massa. All-trans-β-caroteno (70,22 ± 0,4 g.g) e 9-cis-β-caroteno (8,0± 0,1g.g) a partir da biomassa. Visando uma escala industrial a partir do cultivo heterotrofico de 10,000 m/d, estima-se uma producao all-trans-β-caroteno 7.902,5 kg/ano e 4.944,7 kg/ano de 9-cis-βcaroteno apresentando assim valores de alta rentabilidade no beneficiamento deste tipo de pigmento comparados com a producao em meio autotrofico.

Grant IF.00945.2014 and SFRH.BD.119756.2016 (FCT_MCTES), NORTE.08.5369.FSE.000044 (funded by Programa_Operacional_Norte_2020 Fundo Social Europeu), GENE2SKIN Twinning Project, Horizon_2020 (European Commission).

Quinoa (Chenopodium quinoa Willd.) is an annual plant belonging to subfamily Chenopodiaceae containing such granite importance, being considered a pseudocereal. For the quinoa to gain widespread attention, to the point of achieving large scale cultivation in Brazil it is necessary for the studies to advance specially in regards to quality of produced seeds. The main aim of this work was to evaluate potential of quinoa seed’s production cultivated in different seasons, as well as quality of seeds produced on primary and secondary stalks of quinoa plants from BRS Piabiru, produced in the South of Rio Grande do Sul (RS), Brazil. The evaluated seeding seasons were October, November and December, June, July and August of the years 2015/2016, after the plants were taken to evaluation regarding agronomical characters (fresh mass of ramification glomeruli, main glomeruli, fresh mass of stem from the main, fresh mass of stem ramification, fresh mass of leaves, dry mass of stem ramification, stem from the main, glomeruli from the main, stem ramification, of leaves, diameter of stem and panicle lenght) and quality of seeds (germination, test of cold, accelerated aging, and seed’s emergency). Quality of the seed is assured when it’s produced after months of July, August and November, given that quality of seeds from main stem is superior.