1. A panel of in vitro tests to evaluate genotoxic and morphological neoplastic transformation potential on Balb/3T3 cells by pristine and remediated titania and zirconia nanoparticles
- Author
-
Anna Luisa Costa, Sebastiano Di Bucchianico, Andrea Stoccoro, Lucia Migliore, Simona Ortelli, Chiara Uboldi, Magda Blosi, Jessica Ponti, Claudia Placidi, Fabio Coppedè, University of Pisa - Università di Pisa, Università degli Studi di Siena = University of Siena (UNISI), Karolinska Institutet [Stockholm], Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), JRC Institute for Health and Consumer Protection (IHCP), European Commission - Joint Research Centre [Ispra] (JRC), Universitá degli Studi dell’Insubria, CNR Istituto di Scienza e Tecnologia dei Materiali Ceramici (CNR-ISTEC), Consiglio Nazionale delle Ricerche [Roma] (CNR), Universitá degli Studi dell’Insubria = University of Insubria [Varese] (Uninsubria), CNR Istituto di Scienza e Tecnologia dei Materiali Ceramici [Faenza] (CNR | ISTEC), and National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR)
- Subjects
AMORPHOUS SILICA ,0301 basic medicine ,ADSORPTION ,Cell Survival ,Health, Toxicology and Mutagenesis ,BALB 3T3 Cells ,CRYSTAL PHASE ,Metal Nanoparticles ,transforming potential ,NANO ,Toxicology ,medicine.disease_cause ,OXIDE PARTICLES ,Cell Line ,Mice ,03 medical and health sciences ,Genetics ,medicine ,Animals ,CYTOTOXICITY ,Neoplastic transformation ,Viability assay ,BOVINE SERUM-ALBUMIN ,Cytotoxicity ,Genetics (clinical) ,Titanium ,Mutagenicity Tests ,Chemistry ,In vitro toxicology ,DNA ,TiO2 and ZrO2 NP ,Comet assay ,PERIPHERAL-BLOOD LYMPHOCYTES ,TIO2 NANOPARTICLES ,DIOXIDE NANOPARTICLES ,Oxidative Stress ,Cell Transformation, Neoplastic ,030104 developmental biology ,Biochemistry ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,Zirconium ,Genotoxicity ,Micronucleus ,DNA Damage - Abstract
International audience; The FP7 Sanowork project was aimed to minimise occupational hazard and exposure to engineered nanomaterials (ENM) through the surface modification in order to prevent possible health effects. In this frame, a number of nanoparticles (NP) have been selected, among which zirconium (ZrO 2) and titanium (TiO 2) dioxide. In this study, we tested ZrO 2 NP and TiO 2 NP either in their pristine (uncoated) form, or modified with citrate and/or silica on their surface. As benchmark material, Aeroxide® P25 was used. We assessed cytotoxicity, genotoxicity and induction of morphological neoplastic transformation of NP by using a panel of in vitro assays in an established mammalian cell line of murine origin (Balb/3T3). Cell viability was evaluated by means of colony-forming efficiency assay (CFE). Genotoxicity was investigated by cytokinesis-block micronucleus cytome assay (CBMN cyt) and comet assay, and by the use of the restriction enzymes EndoIII and Fpg, oxidatively damaged DNA was detected; finally, the morphological neoplastic transformation of NP was assayed in vitro by cell transformation assay (CTA). Our results show that the surface remediation has not been effective in modifying cyto-and genotoxic properties of the nanomaterials tested; indeed, in the case of remediation of zirconia and titania with citrate, there is a tendency to emphasise the toxic effects. The use of a panel of assays, such as those we have employed, allowing the evaluation of multiple endpoints, including cell transformation, seems particularly advisable especially in the case of long-term exposure effects in the same cell type.
- Published
- 2016
- Full Text
- View/download PDF