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Context Dairy operations have adopted benchmarking as a methodology to rank farms and establish target indexes; however, a connection between benchmarking and farms in the tropics is still warranted. Aims To evaluate the technical and economic quartiles based on farm return on assets (ROA) of three regions (Centre, South and Triangle) of Minas Gerais state, Brazil, and use them to establish benchmarks for dairy farms. Methods We collected data from 128 dairy farms (from January to December of 2019). All properties were part of the Educampo® project/Sebrae-MG. Farms were grouped into the Centre, South and Triangle regions, and subdivided into three groups within each region according to their ROA, where 25% of the farms that presented the lowest ROA were classified as the first quartile, 50% of farms were classified as interquartile and the 25% remaining farms were classified as the fourth quartile. Data were analysed as a randomised block design in a split-plot scheme, where the production systems were blocks, the regions were the main plots and the groups were the split plots. Differences were declared when P ≤ 0.10. Key results Total operating cost ($/L; $ – this currency is in US dollars and it applies throughout the paper); accrual operating cost ($/L); production costs, such as roughage ($/L), hired labour ($/L), percentage of concentrate and hired labour in accrual operating cost (%), were affected by regions and groups. The South and fourth quartile had the greatest total operating cost (0.24 $/L; 0.26 $/L) and accrual operating cost (0.27 $/L; 0.30 $/L), respectively. The majority of economic indexes were higher for Triangle than South and Centre, respectively. The fourth quartile had the greatest net margin (0.09 $/L), profit (0.07 $/L), return on assets (2%) and assets turnover rate (24%). Conclusions We suggest that benchmarks should be established by region, as there were too many variations among regions. In addition, this study demonstrated the importance of understanding the behaviour of the technical and economic indicators to stratify farms based on their return on assets. Implications We evaluated technical and economic indexes from three regions and stratified by ROA. Then, we established benchmarks by regions to better guide the producer in decision-making in dairy operations.

This study evaluated the effect of maternal protein supplementation during mid or late gestation on energy metabolism of the skeletal muscle of beef calves. Sixteen pregnant cows were divided into 3 groups: CTRL (not supplemented); MID (supplemented from 30 to 180 days of gestation); and LATE (supplemented from 181 to 281 days of gestation). The supplement contained 30% crude protein. Thirty days after birth, blood and muscle samples of the calves were collected for analyses of gene expression, proteins, and metabolites. No differences (P ≥ 0.15) in birth weight, performance at weaning, or muscle expression of the genes evaluated (P ≥ 0.21) were observed. Calves born to CTRL cows had a lower ratio (P = 0.03) of p-AMPK/AMPK protein in the skeletal muscle. Calves born to MID cows had lower (P = 0.04) glucose concentration than those born to LATE cows. Changes in p-AMPK/AMPK protein, indicated a possible metabolic inflexibility in the skeletal muscle of calves born to CTRL cows. These results indicated that lack of protein supplementation in pregnant cows alter the energy metabolism of their calves and reflect in a metabolic inflexibility. RESUMO: O objetivo deste estudo foi avaliar o efeito da suplementação proteica materna sobre o metabolismo energético do músculo esquelético de bezerros de corte. Dezesseis matrizes gestantes foram divididas em três grupos: CONTROLE (não suplementado); MÉDIO (suplementados entre 30 e 180 dias de gestação); e FINAL (suplementado entre 181 e 281 dias de gestação). O suplemento continha 30% de proteína bruta e foi fornecido em quantidades totais iguais aos tratamentos. Trinta dias após o nascimento, amostras de sangue e músculo dos bezerros foram coletadas para análises de expressão gênica, abundância de proteínas e metabólitos. Não foram observadas diferenças (P ≥ 0,15) no peso ao nascimento ou parâmetros de desempenho ao desmame, bem como na expressão dos genes avaliados (P ≥ 0,21). Os bezerros nascidos de matrizes do tratamento CONTROLE apresentaram menor proporção (P = 0,03) de proteína p-AMPK/AMPK no músculo esquelético. Os bezerros nascidos de matrizes do tratamento MÉDIO apresentaram concentração de glicose menor (P = 0,04) do que aqueles nascidos de matrizes do tratamento FINAL. Os resultados observados indicam que a ausência de suplementação proteica em matrizes gestantes pode alterar o metabolismo energético da progênie e refletir em uma inflexibilidade metabólica, a qual pode ocasionar limitações quanto à eficiência energética do tecido muscular esquelético e consequentemente, limitar o desempenho da progênie ao longo da fase pós-natal.