16 results on '"Claudia Wiegand"'
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2. Is the Cyanobacterial Bloom Composition Shifting Due to Climate Forcing or Nutrient Changes? Example of a Shallow Eutrophic Reservoir
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Morgane Le Moal, Alexandrine Pannard, Luc Brient, Benjamin Richard, Marion Chorin, Emilien Mineaud, and Claudia Wiegand
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cyanobacteria ,eutrophication ,long term monitoring ,water quality ,Medicine - Abstract
Cyanobacterial blooms in eutrophic freshwater is a global threat to the functioning of ecosystems, human health and the economy. Parties responsible for the ecosystems and human health increasingly demand reliable predictions of cyanobacterial development to support necessary decisions. Long-term data series help with identifying environmental drivers of cyanobacterial developments in the context of climatic and anthropogenic pressure. Here, we analyzed 13 years of eutrophication and climatic data of a shallow temperate reservoir showing a high interannual variability of cyanobacterial development and composition, which is a less occurring and/or less described phenomenon compared to recurrant monospecific blooms. While between 2007–2012 Planktothrix agardhii dominated the cyanobacterial community, it shifted towards Microcystis sp. and then Dolichospermum sp. afterwards (2013–2019). The shift to Microcystis sp. dominance was mainly influenced by generally calmer and warmer conditions. The later shift to Dolichospermum sp. was driven by droughts influencing, amongst others, the N-load, as P remained unchanged over the time period. Both, climatic pressure and N-limitation contributed to the high variability of cyanobacterial blooms and may lead to a new equilibrium. The further reduction of P-load in parallel to the decreasing N-load is important to suppress cyanobacterial blooms and ameliorate ecosystem health.
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- 2021
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3. Daphnia magna Exudates Impact Physiological and Metabolic Changes in Microcystis aeruginosa
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Gorenka Bojadzija Savic, Christine Edwards, Enora Briand, Linda Lawton, Claudia Wiegand, and Myriam Bormans
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cyanobacteria ,secondary metabolites ,PCC7806 ,toxic ,mutant ,infochemicals ,Medicine - Abstract
While the intracellular function of many toxic and bioactive cyanobacterial metabolites is not yet known, microcystins have been suggested to have a protective role in the cyanobacterial metabolism, giving advantage to toxic over nontoxic strains under stress conditions. The zooplankton grazer Daphnia reduce cyanobacterial dominance until a certain density, which may be supported by Daphnia exudates, affecting the cyanobacterial physiological state and metabolites’ production. Therefore, we hypothesized that D. magna spent medium will impact the production of cyanobacterial bioactive metabolites and affect cyanobacterial photosynthetic activity in the nontoxic, but not the toxic strain. Microcystin (MC-LR and des-MC-LR) producing M. aeruginosa PCC7806 and its non-microcystin producing mutant were exposed to spent media of different D. magna densities and culture durations. D. magna spent medium of the highest density (200/L) cultivated for the shortest time (24 h) provoked the strongest effect. D.magna spent medium negatively impacted the photosynthetic activity of M. aeruginosa PCC7806, as well as the dynamics of intracellular and extracellular cyanobacterial metabolites, while its mutant was unaffected. In the presence of Daphnia medium, microcystin does not appear to have a protective role for the strain. On the contrary, extracellular cyanopeptolin A increased in M. aeruginosa PCC7806 although the potential anti-grazing role of this compound would require further studies.
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- 2019
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4. Insights into the molecular mechanisms of pesticide tolerance in the Aporrectodea caliginosa earthworm
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Audrey Barranger, Christophe Klopp, Barbara Le Bot, Gaëlle Saramito, Lise Dupont, Stéphanie Llopis, Claudia Wiegand, Françoise Binet, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Unité de Mathématiques et Informatique Appliquées de Toulouse (MIAT INRAE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de recherche en santé, environnement et travail (Irset), Université d'Angers (UA)-Université de Rennes (UR)-École des Hautes Études en Santé Publique [EHESP] (EHESP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), École des Hautes Études en Santé Publique [EHESP] (EHESP), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and The present study was funded by the Brittany region (France), the François Sommer Foundation (BUZHUG Project - 18XZ316-01D) and the Regional Directorate for the Environment, Planning and Housing (DREAL) through the PHYTOSOL project (N°EJ 2201157402).
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Mitochondrial respiratory chain ,Health, Toxicology and Mutagenesis ,[SDE]Environmental Sciences ,Pesticides residues ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,General Medicine ,Agrosystem ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Transcriptomics ,Toxicology ,Soil biodiversity ,Pollution ,Aporrectodea - Abstract
International audience; Diffuse pollution of the environment by pesticides has become a major soil threat to non-target organisms, such as earthworms for which declines have been reported. However some endogeic species are still abundant and persist in intensively cultivated fields, suggesting they become tolerant to long-term anthropogenic pressure. We thus considered the working hypothesis that populations of Aporrectodea caliginosa earthworms from conventionally managed fields developed a tolerance to pesticides compared with those from organically managed fields. To investigate this hypothesis, we studied earthworm populations of the same genetic lineage from soils that were either lowly or highly contaminated by pesticides to detect any constitutive expression of differentially expressed molecular pathways between these populations. Earthworm populations were then experimentally exposed to a fungicide-epoxiconazole-in the laboratory to identify different molecular responses when newly exposed to a pesticide. State-of-the-art omics technology (RNA sequencing) and bioinformatics were used to characterize molecular mechanisms of tolerance in a non-targeted way. Additional physiological traits (respirometry, growth, bioaccumulation) were monitored to assess tolerance at higher levels of biological organization. In the present study, we generated the de novo assembly transcriptome of A. caliginosa consisting of 64,556 contigs with N50 = 2862 pb. In total, 43,569 Gene Ontology terms were identified for 21,593 annotated sequences under the three main ontologies (biological processes, cellular components and molecular functions). Overall, we revealed that two same lineage populations of A. caliginosa earthworms, inhabiting similar pedo-climatic environment, have distinct gene expression pathways after they long-lived in differently managed agricultural soils with a contrasted pesticide exposure history for more than 22 years. The main difference was observed regarding metabolism, with upregulated pathways linked to proteolytic activities and the mitochondrial respiratory chain in the highly exposed population. This study improves our understanding of the long-term impact of chronic exposure of soil engineers to pesticide residues.
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- 2023
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5. Thermal plasticity and sensitivity to insecticides in populations of an invasive beetle:Cyfluthrin increases vulnerability to extreme temperature
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Martin Holmstrup, Sapho-Lou Marti, Julie Engell Dahl, David Renault, Claudia Wiegand, Hervé Colinet, Université de Rennes (UR), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Aarhus University [Aarhus], Institut Universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), This study was supported by France-Agri-Mer (Research grant ‘Teneblimit’), and by the International Research Project (IRP) ‘Phenomic responses of invertebrates to changing environments and multiple stress (PRICES) funded by InEE-CNRS., Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
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Insecticides ,Environmental Engineering ,Hot Temperature ,Health, Toxicology and Mutagenesis ,heat spike ,0208 environmental biotechnology ,Alphitobius diaperinus ,Population ,02 engineering and technology ,010501 environmental sciences ,Cyfluthrin ,01 natural sciences ,Extreme temperature ,Toxicology ,chemistry.chemical_compound ,Extreme weather ,Heat spike ,Nitriles ,Pyrethrins ,Environmental Chemistry ,Animals ,education ,Extreme Cold ,0105 earth and related environmental sciences ,education.field_of_study ,Pyrethroid ,tolerance ,Thermal variation ,biology ,pyrethroid ,[SDV.BA]Life Sciences [q-bio]/Animal biology ,Public Health, Environmental and Occupational Health ,Temperature ,General Medicine ,General Chemistry ,Pesticide ,biology.organism_classification ,Pollution ,020801 environmental engineering ,Coleoptera ,chemistry ,13. Climate action ,insect ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Insect ,Tolerance ,thermal variation - Abstract
International audience; Climate change increases average temperatures and the occurrence of extreme weather events, in turn accentuating the risk of organism exposure to temperature stress. When thermal conditions become stressful, the sensitivity of insects toward insecticides can be exacerbated. Likewise, exposure of insects to insecticides can subsequently influence their ability to handle stressful temperatures. Here, we investigated the effects of constant temperature and daily heat spikes, in presence/absence of insecticide treatment (cyfluthrin), on the condition (impairment of mobility) and thermal tolerance to cold (-6 °C) and heat (42.5 °C) of the terrestrial beetle Alphitobius diaperinus. The responses of insects from four populations (three farm-collected populations, one laboratory population) to different durations of extreme temperature exposure were compared. The results showed that the laboratory population was generally more sensitive to extreme cold and heat temperatures, with less than 50% of adults recovering after an exposure at -6 or +42.5 °C for 3h. Significant differences in the level of thermal tolerance were also found among insects from poultry farms. Cyfluthrin exposure incurred detrimental effects to insects’ condition in all but one population. For two out of the four populations, mobility impairment was increased when adults were exposed to daily heat spikes (6 h per day at 38 °C) and cyfluthrin simultaneously, compared to cyfluthrin exposure at constant temperatures; yet, no significant interaction between the two stressors was found. Finally, using one farm collected population, effects of pre-exposure to cyfluthrin on extreme temperature tolerance provided another example of the toxicant-induced climate sensitivity in insects.
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- 2021
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6. Cell free Microcystis aeruginosa spent medium affects Daphnia magna survival and stress response
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Enora Briand, Gorenka Bojadzija Savic, Linda A. Lawton, Christine Edwards, Hervé Colinet, Claudia Wiegand, Myriam Bormans, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Robert Gordon University (RGU), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Gorenka Bojadzija Savic was supported by a Presidential scholarship from the University of Rennes 1. The project benefited from funding from the Centre National de la Recherche Scientifique (CNRS) Initiative Structurante Ecosphère continentale et côtière (EC2CO) under the Interactions métaboliques entre cyanobactéries et daphnies (MICYDA) project. Gorenka Bojadzija Savic was granted a mobility grant from the Doctoral School EGAAL (Bretagne-Loire, France) to visit the Robert Gordon University in Aberdeen, Scotland for metabolites analyses., Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,Antioxidant ,Microcystis ,Microcystins ,medicine.medical_treatment ,Daphnia magna ,Toxicology ,medicine.disease_cause ,Cyanobacteria ,01 natural sciences ,PCC7806 ,Zooplankton ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Animals ,Microcystis aeruginosa ,Transcriptomics ,Ecosystem ,[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography ,Glutathione Transferase ,chemistry.chemical_classification ,0303 health sciences ,biology ,010604 marine biology & hydrobiology ,Glutathione peroxidase ,Secondary metabolites ,030302 biochemistry & molecular biology ,fungi ,Glutathione ,Metabolism ,biology.organism_classification ,6. Clean water ,[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology ,chemistry ,Biochemistry ,Daphnia ,13. Climate action ,Catalase ,Oxidative stress ,biology.protein ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis - Abstract
International audience; Primary consumers in freshwater ecosystems, such as the zooplankton organism Daphnia magna, are highly affected by cyanobacteria, both as they may use it as a food source but also by cyanobacterial metabolites present in the water. Here, we investigate the impacts of cyanobacterial metabolites focussing on the environmental realistic scenario of the naturally released mixture without crushing cyanobacterial cells or their uptake as food. Therefore, D. magna were exposed to two concentrations of cell free cyanobacterial spent medium from Microcystis aeruginosa PCC 7806 to represent higher and lower ecologically-relevant concentrations of cyanobacterial metabolites. Including microcystin-LR, 11 metabolites have been detected of which 5 were quantified. Hypothesising concentration and time dependent negative impact, survival, gene expression marking digestion and metabolism, oxidative stress response, cell cycle and molting as well as activities of detoxification and antioxidant enzymes were followed for 7 days. D. magna suffered from oxidative stress as both catalase and glutathione S-transferase enzyme activities significantly decreased, suggesting enzyme exhaustibility after 3 and 7 days. Moreover, gene-expressions of the 4 stress markers (glutathione S-transferase, glutathione peroxidase, catalase and thioredoxin) were merely downregulated after 7 days of exposure. Energy allocation (expression of glyceraldehyde-3-phosphate dehydrogenase) was increased after 3 days but decreased as well after 7 days exposure. Cell cycle was impacted time dependently but differently by the two concentrations, along with an increasing downregulation of myosin heavy chain responsible for cell arrangement and muscular movements. Deregulation of nuclear hormone receptor genes indicate that D. magna hormonal steering including molting seemed impaired despite no detection of microviridin J in the extracts. As a consequence of all those responses and presumably of more than investigated molecular and physiological changes, D. magna survival was impaired over time, in a concentration dependent manner. Our results confirm that besides microcystin-LR, other secondary metabolites contribute to negative impact on D. magna survival and stress response.
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- 2021
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7. How can interspecific interactions in freshwater benthic macroinvertebrates modify trace element availability from sediment?
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Martine Bouhnik-Le Coz, Ana María Gagneten, Claudia Wiegand, Victoria Soledad Andrade, Mathieu Pédrot, Alexandrine Pannard, Christophe Piscart, Universidad Nacional del Litoral [Santa Fe] (UNL), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Géosciences Rennes (GR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Universidad Nacional del Litoral, Fond Français pour l’Environnement Urbain, LTSER France Zone Atelier Armorique, Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), and Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 1 (UR1)
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Geologic Sediments ,Environmental Engineering ,Amphipoda ,Health, Toxicology and Mutagenesis ,0208 environmental biotechnology ,Fresh Water ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Macroinvertebrate ,Rare earth element ,Environmental Chemistry ,Animals ,14. Life underwater ,Oligochaeta ,0105 earth and related environmental sciences ,biology ,Chemistry ,fungi ,Public Health, Environmental and Occupational Health ,Sediment ,General Medicine ,General Chemistry ,Mussel ,biology.organism_classification ,Bivalvia ,Pollution ,Invertebrates ,6. Clean water ,020801 environmental engineering ,Trace Elements ,Benthic zone ,Tubifex tubifex ,Environmental chemistry ,Bioaccumulation ,Trace element ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,Bioturbation ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Water Pollutants, Chemical - Abstract
International audience; This study aimed to assess how bioturbation by freshwater benthic macroinvertebrates with different biological traits alone or in combination could modify trace elements (TE) fate between sediment and water, and if water TE concentration and animal TE content impair their body stores. Three macroinvertebrate species were exposed to TE contaminated sediment for 7 days: the omnivorous Echinogammarus berilloni (Amphipoda), the sediment feeding Tubifex tubifex (Oligochaeta) and the filter feeding Pisidium sp. (Bivalvia). Treatments were one without invertebrates (control), two with amphipods or mussels alone, and the combinations amphipod-mussel, and amphipod-mussel-worms. Water TE concentration increased significantly in 2 or 3 species mesocosms, concerning mainly Rare Earth Elements, Cr, U and Pb, known to be associated to the colloidal phase. By contrast, water soluble TE were not affected by animals. For both, amphipods and mussels, TE body content increased with the number of coexisting species. For amphipods, this increase concerned both, soluble and colloid-associated TE, possibly due to intense contact and feeding from sediment and predation on tubificids. TE bioaccumulation in mussel was less important and characterized by soluble TE, with water filtration as most plausible uptake route. Protein, triglyceride and Whole Body Energy Budget increased in amphipods with the number of coexisting species (probably by feeding on mussels’ feces and tubificids) whereas triglycerides declined in mussels (presumably filtration was disturbed by amphipods). This study highlights interspecific interactions as key drivers explaining both: TE bioturbation, depending on their water solubility or colloidal association, and the exposure/contamination of species through another species activity.
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- 2020
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8. Cross talk: Two way allelopathic interactions between toxic Microcystis and Daphnia
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Christine Edwards, Claudia Wiegand, Myriam Bormans, Enora Briand, Linda A. Lawton, Gorenka Bojadzija Savic, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Robert Gordon University (RGU), Institut Français de Recherche pour l'Exploitation de la Mer - Atlantique (IFREMER Atlantique), University of Rennes 1, Centre National de la Recherche Scientifique (CNRS) Initiative Structurante Ecosphere continentale et cotiere (EC2CO) under the Interactions metaboliques entre cyanobacteries et daphnies (MICYDA) project, Doctoral School University of Rennes 1, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,Cyanobacteria ,Microcystis ,[SDV]Life Sciences [q-bio] ,Daphnia magna ,Zoology ,Plant Science ,Microcystin ,010501 environmental sciences ,Aquatic Science ,01 natural sciences ,Daphnia ,PCC7806 ,Zooplankton ,Extracellular ,Animals ,Microcystis aeruginosa ,14. Life underwater ,Ecosystem ,Allelopathy ,reproductive and urinary physiology ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,biology ,010604 marine biology & hydrobiology ,Secondary metabolites ,fungi ,biology.organism_classification ,Toxic ,chemistry ,Oxidative stress ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology - Abstract
International audience; Due to eutrophication, freshwater ecosystems frequently experience cyanobacterial blooms, many of which produce bioactive metabolites that can affect vertebrates and invertebrates life traits. Zooplankton are able to develop tolerance as a physiological response to cyanobacteria and their bioactive compounds, however, this comes with energetic cost that in turn influence Daphnia life traits and may impair populations. Vice versa, it has been suggested that Daphnia are able to reduce cyanobacterial dominance until a certain cyanobacterial density; it remains unclear whether Daphnia metabolites alone influence the physiological state and bioactive metabolites production of cyanobacteria. Hence, this study investigates mutual physiological reactions of toxic Microcystis aeruginosa PCC7806 and Daphnia magna. We hypothesize that a) the presence of D. magna will negatively affect growth, increase stress response and metabolites production in M. aeruginosa PCC7806 and b) the presence of M. aeruginosa PCC7806 will negatively affect physiological responses and life traits in D. magna. In order to test these hypotheses experiments were conducted in a specially designed co-culture chamber that allows exchange of the metabolites without direct contact. A clear mutual impact was evidenced. Cyanobacterial metabolites reduced survival of D. magna and decreased oxidative stress enzyme activity. Simultaneously, presence of D. magna did not affect photosynthetic activity. However, ROS increase and tendencies in cell density decrease were observed on the same day, suggesting possible energy allocation towards anti-oxidative stress enzymes, or other protection mechanisms against Daphnia infochemicals, as the strain managed to recover. Elevated concentration of intracellular and overall extracellular microcystin MC-LR, as well as intracellular concentrations of aerucyclamide A and D in the presence of Daphnia, indicating a potential protective or anti-grazing function. However, more research is needed to confirm these findings.
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- 2020
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9. Responses of a tropical micro-crustacean, Daphnia lumholtzi, upon exposures to dissolved toxins and living cells of cyanobacteria
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Khuong Van Dinh, Ba Trung Bui, Thi My Chi Vo, Claudia Wiegand, Thanh-Son Dao, Duy Tan University (DTU), Wageningen University and Research [Wageningen] (WUR), Vietnam National University - Ho Chi Minh City (VNU-HCM), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Washington State University (WSU), We would like to thank Prof. Tham Hoang from Loyola University Chicago for his assistance on the calculation of median lethal concentration (24h- and 48h-LC50). This research is funded by Vietnam National Foundation for Science and Technology Development (NAFOSTED) under grant number 106-NN.04-2014.69., Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Centre Asiatique de Recherche sur l'Eau (CARE), and Hochiminh City University of Technology
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0106 biological sciences ,Cyanobacteria ,Aquatic Ecology and Water Quality Management ,Microcystins ,Survival proportion ,Soil Science ,Zoology ,Acute lethal concentration ,Plant Science ,010501 environmental sciences ,01 natural sciences ,Daphnia ,Dominance (ecology) ,Daphnia lumholtzi ,Microcystis aeruginosa ,14. Life underwater ,Clutch size ,0105 earth and related environmental sciences ,General Environmental Science ,biology ,010604 marine biology & hydrobiology ,Aquatic ecosystem ,Reproduction ,fungi ,Cylindrospermopsis curvispora ,Aquatische Ecologie en Waterkwaliteitsbeheer ,biology.organism_classification ,Crustacean ,[SDE]Environmental Sciences ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Bloom - Abstract
International audience; The mass development and expansion of cyanobacteria release cyanotoxins in the aquatic environment and cause serious problems for grazers such as micro-zooplankton. In contrast to aquatic ecosystems in temperate regions, impacts of cyanobacteria and their toxins on tropical micro-crustaceans are relatively understudied. In this study, acute and chronic effects of pure microcystin-LR (MC-LR), a crude extract of water bloom sample with a dominance of Microcystis aeruginosa containing microcystins (MCE) and living cells of Cylindrospermopsis curvispora were tested on a tropical micro-crustacean, Daphnia lumholtzi. The 24 h- and 48 h-LC50 values for MC-LR ranged from 247–299, and 331–409 μg MCE L−1, respectively. Exposures to 1–25 μg MCE L−1 decreased survival, fecundity, and reproduction of D. lumholtzi. The impacts of C. curvispora cells on life-history traits of D. lumholtzi were density-dependent and more severe than the impacts of dissolved microcystins. It could be that the adverse effects of C. curvispora on D. lumholtzi are linked to a combination of potential toxic metabolites, mal-nutrients, feeding and swimming interference. Daphnia lumholtzi used in this study is more sensitive to microcystins compared to Daphnia species from temperate regions. Therefore, we highly suggest using D. lumholtzi as a model species for toxicity testing and monitoring on water quality, particularly in tropical countries.
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- 2020
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10. Strategies and Consequences of Indigenous and Invasive Freshwater Mussels Living in Cyanobacterial and Anthropogenic Impacted Waters
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Claudia Wiegand, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Elisabeth Gross, Jeanne Garric, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,Macroinvertebrate Communities ,Aquatic Organisms ,Toxin Microcystin-lr ,STREAMS ,010501 environmental sciences ,01 natural sciences ,Ecosystem services ,Water column ,Nutrient ,Organic matter ,14. Life underwater ,ComputingMilieux_MISCELLANEOUS ,Corbicula-fluminea ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Ecology ,010604 marine biology & hydrobiology ,Acute Toxicity ,fungi ,Sediment ,Pelagic zone ,Commercial Formulation ,15. Life on land ,6. Clean water ,Zebra Mussel ,chemistry ,13. Climate action ,Benthic zone ,Multixenobiotic Resistance ,[SDV.TOX]Life Sciences [q-bio]/Toxicology ,Environmental science ,Limnoperna-fortunei ,Dreissena-polymorpha - Abstract
Most indigenous European and North American mussels live on or partially burrowed in sediments of lakes and slow-flowing streams and rivers. They nourish merely non-selectively by filtering suspended particles (and the bacteria colonizing them), and with this activity, they provide the ecosystem service of water clearance, particle processing and nutrient dynamics (release and biodeposition), and thus link the pelagic compartment to the benthic one by transferring material and nutrients from the water column to the sediment. Moreover, by their turbation activity, they oxygenize the upper layers of the sediment aiding bacterial degradation of organic matter.
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- 2019
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11. Sensitivity of a tropical micro-crustacean (Daphnia lumholtzi) to trace metals tested in natural water of the Mekong River
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Thi-My-Chi Vo, Truong-Giang Vo, Cong-Thanh Dao, Claudia Wiegand, Ly-Sy-Phu Nguyen, Khuong Van Dinh, Van-Dong Nguyen, Thanh-Son Nguyen, Thanh-Son Dao, Ba-Trung Bui, Vu-Nam Le, Thi-Hien To, HoChiMinh-City University of Technology, Ho Chi Minh City University of Technology (HCMUT), University of Science, Vietnam National University, Institute for Environment and Resources, Vietnam National University, Technical University of Denmark, National Institute of Aquatic Resources, Department of Freshwater Aquaculture, Nha Trang University, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Vietnam National University - Hochiminh City [B2014-48-01], Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
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reproductive toxicity ,silver exposure ,0106 biological sciences ,copper toxicity ,Environmental Engineering ,dissolved organic-carbon ,magna ,Biodiversity ,biotic ligand model ,010501 environmental sciences ,01 natural sciences ,Daphnia ,Rivers ,Metals, Heavy ,Dissolved organic carbon ,Toxicity Tests, Acute ,medicine ,Animals ,Environmental Chemistry ,Daphnia lumholtzi ,Toxicity Tests, Chronic ,Waste Management and Disposal ,Chronic toxicity ,0105 earth and related environmental sciences ,dietary copper ,biology ,Ecology ,Chemistry ,010604 marine biology & hydrobiology ,zinc ,Copper toxicity ,Biotic Ligand Model ,15. Life on land ,biology.organism_classification ,medicine.disease ,Pollution ,Acute toxicity ,nickel toxicity ,Vietnam ,Environmental chemistry ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
International audience; Metal contamination is one of the major issues to the environment worldwide, yet it is poorly known how exposure to metals affects tropical species. We assessed the sensitivity of a tropical micro-crustacean Daphnia lumholtzi to three trace metals: copper (Cu), zinc (Zn) and nickel (Ni). Both, acute and chronic toxicity tests were conducted with metals dissolved in in situ water collected from two sites in the lower part of the Mekong River. In the acute toxicity test, D. lumholtzi neonates were exposed to Cu (3-30 mu g L-1), Zn (50-540 mu g L-1) or Ni (46-2356 mu g L-1) for 48 h. The values of median lethal concentrations (48 h-LC50) were 11.57-16.67 mu g Cu L-1, 179.3-280.9 mu g Zn L-1, and 1026-1516 mu g Ni L-1. In the chronic toxicity test, animals were exposed to Cu (3 and 4 mu g L-1), Zn (50 and 56 mu g L-1), and Ni (six concentrations from 5 to 302 mu g L-1) for 21 days. The concentrations of 4 mu g Cu L-1 and 6 mu g Ni L-1 enhanced the body length of D. lumholtzi but 46 mu g Ni L-1 and 50 mu g Zn L-1 resulted in a strong mortality, reduced the body length, postponed the maturation, and lowered the fecundity. The results tentatively suggest that D. lumholtzi showed a higher sensitivity to metals than related species in the temperate region. The results underscore the importance of including the local species in ecological risk assessment in important tropical ecosystems such as the Mekong River to arrive at a better conservational and management plan and regulatory policy to protect freshwater biodiversity from metal contamination. (C) 2016 Elsevier B.V. All rights reserved.
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- 2017
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12. List of Authors
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Claude Amiard-Triquet, Amélie Châtel, Davide Degli-Esposti, Jeanne Garric, Laure Giambérini, Elisabeth Gross, Laëtitia Minguez, Catherine Mouneyrac, Odette Prat, Messika Revel, and Claudia Wiegand
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- 2019
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13. Transgenerational effects of cyanobacterial toxins on a tropical micro-crustacean Daphnia lumholtzi across three generations
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Khuong Van Dinh, Thanh-Son Dao, Ba-Trung Bui, Thi-My-Chi Vo, Claudia Wiegand, Ho Chi Minh City University of Technology (HCMUT), Duy Tan University (DTU), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institute for Environment and Resources, Vietnam National University - Ho Chi Minh City (VNU-HCM), Nha Trang University, Technical University of Denmark [Lyngby] (DTU), 106-NN.04-2014.69, National Foundation for Science and Technology Development, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), and Danmarks Tekniske Universitet = Technical University of Denmark (DTU)
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0106 biological sciences ,Aquatic Ecology and Water Quality Management ,Microcystins ,Health, Toxicology and Mutagenesis ,Bacterial Toxins ,Drug Resistance ,Zoology ,Microcystin ,010501 environmental sciences ,Cyanobacteria ,Toxicology ,01 natural sciences ,Zooplankton ,Freshwater ecosystem ,Life history theory ,SDG 13 - Climate Action ,Animals ,Daphnia lumholtzi ,14. Life underwater ,Adaptation ,SDG 15 - Life on Land ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Tropical Climate ,Cyanobacteria Toxins ,biology ,Reproduction ,010604 marine biology & hydrobiology ,Aquatic ecosystem ,General Medicine ,Life history traits ,Aquatische Ecologie en Waterkwaliteitsbeheer ,biology.organism_classification ,Fecundity ,Pollution ,6. Clean water ,Lakes ,Daphnia ,chemistry ,Female ,Marine Toxins ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Tolerance - Abstract
Climate change and human activities induce an increased frequency and intensity of cyanobacterial blooms which could release toxins to aquatic ecosystems. Zooplankton communities belong to the first affected organisms, but in tropical freshwater ecosystems, this issue has yet been poorly investigated. We tested two questions (i) if the tropical Daphnia lumholtzi is capable to develop tolerance to an ecologically relevant concentration of purified microcystin-LR and microcystins from cyanobacterial extract transferable to F1 and F2 generations? And (ii) would F1 and F2 generations recover if reared in toxin-free medium? To answer these questions, we conducted two full factorial mutigenerational experiments, in which D. lumholtzi was exposed to MC-LR and cyanobacterial extract at the concentration of 1 μg L−1 microcystin continuously for three generations. After each generation, each treatment was spit into two: one reared in the control (toxin free) while the other continued in the respective exposure. Fitness-related traits including survival, maturity age, body length, and fecundity of each D. lumholtzi generation were quantified. Though there were only some weak negative effects of the toxins on the first generation (F0), we found strong direct, accumulated and carried-over impacts of the toxins on life history traits of D. lumholtzi on the F1 and F2, including reductions of survival, and reproduction. The maturity age and body length showed some inconsistent patterns between generations and need further investigations. The survival, maturity age (for extract), and body length (for MC-LR) were only recovered when offspring from toxin exposed mothers were raised in clean medium for two generations. Chronic exposure to long lasting blooms, even at low density, evidently reduces survival of D. lumholtzi in tropical lakes and reservoirs with ecological consequences. Exposure to environmentally relevant concentrations of cyanobacterial toxins for 3 generations tropical Daphnia lumholtzi developed no or marginal tolerance.
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- 2018
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14. Virtual laboratories in science education: students’ motivation and experiences in two tertiary biology courses
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Nadia Dyrberg, Alexander H. Treusch, Claudia Wiegand, University of Southern Denmark (SDU), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), E-Learning Project Fund, University of Southern Denmark, Laboratory for Coherent Education and Learning, University of Southern Denmark and Department of Biology, University of Southern Denmark, Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
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Self-efficacy ,Medical education ,Science instruction ,Multimedia ,4. Education ,05 social sciences ,education ,050301 education ,050109 social psychology ,Virtual laboratory ,computer.software_genre ,pre-laboratory preparation ,Science education ,Education ,motivation ,Virtual Laboratory ,ComputingMilieux_COMPUTERSANDEDUCATION ,0501 psychology and cognitive sciences ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,General Agricultural and Biological Sciences ,0503 education ,computer ,self-efficacy - Abstract
International audience; Potential benefits of simulations and virtual laboratory exercises in natural sciences have been both theorised and studied recently. This study reports findings from a pilot study on student attitude, motivation and self-efficacy when using the virtual laboratory programme Labster. The programme allows interactive learning about the workflows and procedures of biological and biochemical experiments, the operation of relevant apparatuses, including the ability to adjust parameters, and the production of results. The programme was used as supplement to mandatory laboratory exercises in two undergraduate courses (i. microbiology and ii. pharmaceutical toxicology) at the University of Southern Denmark. With a theoretical basis in motivational theories, students’ (n = 73) motivation and attitude towards the virtual exercises were evaluated. After completing virtual laboratory cases, the students felt significantly more confident and comfortable operating laboratory equipment, but they did not feel more motivated to engage in virtual laboratories compared to real laboratories. Teachers observed that students were able to participate in discussions at higher levels than in previous years where the programme was not used. The study concludes that virtual laboratories have the potential to improve students’ pre-laboratory preparation. © 2016 Royal Society of Biology.
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- 2017
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15. Influence of environmental factors on cyanobacterial biomass and microcystin concentration in the Dau Tieng Reservoir, a tropical eutrophic water body in Vietnam
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Thanh-Son Dao, Utsumi Motoo, Jorge Nimptsch, Claudia Wiegand, Thanh-Luu Pham, Ngoc-Dang Tran, Instituto de Ciencias Marinas y Limnológicas, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
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0106 biological sciences ,Cyanobacteria ,Cyanotoxins ,Microcystin ,Bayesian model average ,010501 environmental sciences ,Aquatic Science ,01 natural sciences ,Eutrophic water ,Temperate climate ,Chroococcales ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Abiotic component ,biology ,Ecology ,010604 marine biology & hydrobiology ,Community structure ,Cyanotoxin ,biology.organism_classification ,6. Clean water ,total phosphorus ,chemistry ,13. Climate action ,Environmental chemistry ,harmful cyanobacterial blooms ,Environmental science ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology - Abstract
International audience; Cyanobacterial blooms can be harmful to environmental and human health due to the production of toxic secondary metabolites, known as cyanotoxins. Microcystins (MCs), one of the most widespread class of cyanotoxins in freshwater, have been found to be positively correlated with cyanobacterial biomass as well as with nitrogen and phosphorus concentrations in temperate lakes. However, in tropical water bodies,cyanobacterial density and cyanotoxin correlation to environmental factors is not fully understood. In the present study, we examined the effects of total nitrogen and total phosphorus (TP) concentrations among other environmental parameters on cyanobacterial community structure and MC concentrations in the Dau Tieng reservoir, a tropical, eutrophic water body in Southern Vietnam. Cyanobacterial biomass and MC content were monitored monthly from March 2012 to February 2013, when MCs were present in the Dau Tieng Reservoir. The highest concentrations of intracellular MCs were found in September and February when cyanobacteria biomass reached maximum values, with 2.50 and 2.13 mg MC.L–1, respectively. Principle component analysis and redundancy analysis showed that MC concentration was positively correlated with the biomass of the cyanobacterial order Chroococcales, whereas TP was the primary abiotic factor influencing cyanobacterial biomass and MC concentrations in the Dau Tieng Reservoir. In addition, Bayesian model average analysis was used to construct a prediction model of MCs using cyanobacterial biomass and environmental variables revealing a suite of useful predictive factors for MCs in the Dau Tieng Reservoir, includingwater temperature, TP and the biomass of Chroococcales.
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- 2017
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16. Accumulation and detoxication responses of the gastropod Lymnaea stagnalis to single and combined exposures to natural (cyanobacteria) and anthropogenic (the herbicide RoundUp(®) Flash) stressors
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Linda A. Lawton, Emilie Lance, Claudia Gérard, Bente Frost Holbech, Julia Desprat, Myriam Bormans, Claudia Wiegand, Christine Edwards, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE), INSTITUTE FOR INNOVATION, DESIGN & SUSTAINABILITY (IDEAS), University Robert Gordon, University of Southern Denmark (SDU), financial support of the European University of Brittany, Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), Robert Gordon University (RGU), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
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0301 basic medicine ,Cyanobacteria ,Microcystins ,Health, Toxicology and Mutagenesis ,Glycine ,Lymnaea stagnalis ,010501 environmental sciences ,Aquatic Science ,medicine.disease_cause ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Biotransformation ,Accumulation ,Stress, Physiological ,Botany ,medicine ,Animals ,Food science ,Gastropod ,0105 earth and related environmental sciences ,Glutathione Transferase ,Lymnaea ,chemistry.chemical_classification ,biology ,Herbicides ,Glyphosate (Roundup® Flash) ,biology.organism_classification ,Oxidative Stress ,030104 developmental biology ,Enzyme ,chemistry ,13. Climate action ,Catalase ,Glyphosate ,Inactivation, Metabolic ,biology.protein ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,Oxidative stress ,Biomarkers ,Water Pollutants, Chemical - Abstract
International audience; Freshwater gastropods are increasingly exposed to multiple stressors in the field such as the herbi- cide glyphosate in Roundup formulations and cyanobacterial blooms either producing or not producing microcystins (MCs), potentially leading to interacting effects. Here, the responses of Lymnaea stagnalis to a 21-day exposure to non-MC or MC-producing (33 ug L−1 ) Planktothrix agardhii alone or in combination with the commercial formulation RoundUp® Flash at a concentration of 1 ug L−1 glyphosate, followed by 14 days of depuration, were studied via i) accumulation of free and bound MCs in tissues, and ii) activities of anti-oxidant (catalase CAT) and biotransformation (glutathione-S-transferase GST) enzymes. During the intoxication, the cyanobacterial exposure induced an early increase of CAT activity, independently of the MC content, probably related to the production of secondary cyanobacterial metabolites. The GST activity was induced by RoundUp® Flash alone or in combination with non MC-producing cyanobacte- ria, but was inhibited by MC-producing cyanobacteria with or without RoundUp® Flash. Moreover, MC accumulation in L. stagnalis was 3.2 times increased when snails were concomitantly exposed to MC- producing cyanobacteria with RoundUp®, suggesting interacting effects of MCs on biotransformation processes. The potent inhibition of detoxication systems by MCs and RoundUp® Flash was reversible during the depuration, during which CAT and GST activities were significantly higher in snails previ- ously exposed to MC-producing cyanobacteria with or without RoundUp® Flash than in other conditions, probably related to the oxidative stress caused by accumulated MCs remaining in tissues.
- Published
- 2015
- Full Text
- View/download PDF
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