6 results on '"Christine Arnould"'
Search Results
2. Dual Cherenkov Radiation-Induced Near-Infrared Luminescence Imaging and Photodynamic Therapy toward Tumor Resection
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Vivian Lioret, Richard A. Decréau, Pierre-Simon Bellaye, Christine Arnould, Bertrand Collin, Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] (ICMUB), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut de Chimie du CNRS (INC), Centre Régional de Lutte contre le cancer Georges-François Leclerc [Dijon] (UNICANCER/CRLCC-CGFL), UNICANCER, Agroécologie [Dijon], Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and French Ministry Enseignement Superieur et de la Recherche Region Bourgogne-Franche-Comte DiMaCell platform for confocal microscopy studies Camille Drouet for radioactivity measurements PARI2 program 3MIM program CNRS Chaire d'Excellence European Union through the PO FEDER-FSE Bourgogne 2014/2020 program French Government ANR-10-EQPX-05-01/IMAPPI EquipexFondation de Cooperation Scientifique Bourgogne Franche-Comte Canceropole Est
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Luminescence ,Light ,Infrared Rays ,Infrared ,Phthalocyanines ,medicine.medical_treatment ,Bodipy ,Photodynamic therapy ,01 natural sciences ,Energy-transfer ,Mice ,03 medical and health sciences ,Optics ,Unresected ,Cell Line, Tumor ,Quantum Dots ,Drug Discovery ,medicine ,Animals ,Cherenkov radiation ,030304 developmental biology ,0303 health sciences ,Chemistry ,business.industry ,Optical Imaging ,Cerenkov Radiation ,[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences ,0104 chemical sciences ,Recognition ,010404 medicinal & biomolecular chemistry ,Photochemotherapy ,Bright Enough ,Radiance ,Nanoparticles ,Molecular Medicine ,Antenna (radio) ,Reactive Oxygen Species ,business ,Visible spectrum - Abstract
International audience; Cherenkov radiation (CR), the blue light seen in nuclear reactors, is emitted by some radiopharmaceuticals. This study showed that (1) a portion of CR could be transferred in the region of the optical spectrum, where biological tissues are most transparent: as a result, upon radiance amplification in the near-infrared window, the detection of light could occur twice deeper in tissues than during classical Cherenkov luminescence imaging and (2) Cherenkov-photodynamic therapy (CR-PDT) on cells could be achieved under conditions mimicking unlimited depth using the CR-embarked light source, which is unlike standard PDT, where light penetration depth is limited in biological tissues. Both results are of utmost importance for simultaneous applications in tumor resection and post-resection treatment of remaining unresected margins, thanks to a molecular construct designed to raise its light collection efficiency (i.e., CR energy transfer) by conjugation with multiple CR-absorbing (water-soluble) antenna followed by intramolecular-FRET/TBET energy transfers.
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- 2020
3. Imaging plant tissues: advances and promising clearing practices
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Mathilde Hériché, Christine Arnould, Daniel Wipf, and Pierre-Emmanuel Courty
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Imaging, Three-Dimensional ,Optical Imaging ,Agriculture ,Plant Science ,Plants - Abstract
The study of the organ structure of plants and understanding their physiological complexity requires 3D imaging with subcellular resolution. Most plant organs are highly opaque to light, and their study under optical sectioning microscopes is therefore difficult. In animals, many protocols have been developed to make organs transparent to light using clearing protocols (CPs). By contrast, clearing plant tissues is challenging because of the presence of fibers and pigments. We describe progress in the development of plant CPs over the past 20 years through a modified taxonomy of CPs based on their physical and optical parameters that affect tissue properties. We also discuss successful approaches that combine CPs with new microscopy methods and their future applications in plant science research.
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- 2021
4. New clearing protocol for tannic roots optical imaging
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Christine Arnould, Courty Pierre-Emmanuel, Mathilde Hériché, and Daniel Wipf
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Optical imaging ,Imaging, Three-Dimensional ,Optical Imaging ,Clearing ,Plant Science ,Biology ,Protocol (object-oriented programming) ,Plant Roots ,Biomedical engineering - Published
- 2021
5. Sugar exchanges in arbuscular mycorrhiza: RiMST5 and RiMST6, two novel Rhizophagus irregularis monosaccharide transporters, are involved in both sugar uptake from the soil and from the plant partner
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Christine Arnould, Nassima Ait Lahmidi, Odile Chatagnier, Daniel Wipf, Laurent Bonneau, Pierre-Emmanuel Courty, Daphnée Brulé, Joan Doidy, Guido Lingua, Graziella Berta, Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Department of Environmental Sciences, Botany, Zurich Basel Plant Science Center, University of Basel (Unibas)-Universität Zürich [Zürich] = University of Zurich (UZH)-University of Basel (Unibas)-Universität Zürich [Zürich] = University of Zurich (UZH), and Amedeo Avogadro University of Eastern Piedmont
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0106 biological sciences ,0301 basic medicine ,Rhizophagus irregularis ,Light ,Physiology ,[SDV]Life Sciences [q-bio] ,Plant Science ,arbuscular mycorrhizal fungus ,01 natural sciences ,rhizophagus irregularis ,Glomeromycota ,Soil ,Gene Expression Regulation, Plant ,Mycorrhizae ,Medicago ,Phylogeny ,2. Zero hunger ,Mutualism (biology) ,Fungal protein ,Reverse Transcriptase Polymerase Chain Reaction ,glucose specific ,Monosaccharides ,food and beverages ,high affinity H+ co-transporter ,high affinity transporter ,Arbuscular mycorrhiza ,Biochemistry ,[SDE]Environmental Sciences ,Fungus ,Saccharomyces cerevisiae ,Biology ,Fungal Proteins ,03 medical and health sciences ,Symbiosis ,Stress, Physiological ,Botany ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,RNA, Messenger ,Obligate ,Cell Membrane ,Genetic Complementation Test ,fungi ,MST5 ,MST6 ,Membrane Transport Proteins ,15. Life on land ,monosaccharide transporter ,biology.organism_classification ,030104 developmental biology ,Glucose ,010606 plant biology & botany - Abstract
SPE IPM INRA UB CT1; International audience; Arbuscular mycorrhizal (AM) fungi are associated with about 80% of land plants. AM fungi provide inorganic nutrients to plants and in return up to 20% of the plant-fixed CO2 is transferred to the fungal symbionts. Since AM fungi are obligate biotrophs, unraveling how sugars are provided to the fungus partner is a key for understanding the functioning of the symbiosis. In this study, we identified two new monosaccharide transporters from Rhizophagus irregularis (RiMST5 and RiMST6) that we characterized as functional high affinity monosaccharide transporters. RiMST6 was characterized as a glucose specific, high affinity H(+) co-transporter. We provide experimental support for a primary role of both RiMST5 and RiMST6 in sugar uptake directly from the soil. The expression patterns of RiMSTs in response to partial light deprivation and to interaction with different host plants were investigated. Expression of genes coding for RiMSTs was transiently enhanced after 48h of shading and was unambiguously dependent on the host plant species. These results cast doubt on the 'fair trade' principle under carbon-limiting conditions. Therefore, in light of these findings, the possible mechanisms involved in the modulation between mutualism and parasitism in plant-AM fungus interactions are discussed. Copyright 2016 Elsevier Masson SAS. All rights reserved.
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- 2016
6. Preservation of viability and anti-Listeria activity of lactic acid bacteria, Lactococcus lactis and Lactobacillus paracasei, entrapped in gelling matrices of alginate or alginate/caseinate
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Adem Gharsallaoui, Rémi Saurel, Elodie Noirot, Aline Bonnotte, Christine Arnould, Olfa Beji, Jeannine Lherminier, Lucie Léonard, Nadia Oulahal, Pascal Degraeve, Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BIODYMIA), Isara-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Conseil Regional de Bourgogne, and Syndicat Mixte du technopole Alimentec (Fonds de Developpement de la Recherche)
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0106 biological sciences ,Lactobacillus paracasei ,Microorganism ,Population ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,010608 biotechnology ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,Lactic acid bacteria ,anti-Listeria spp. activity ,Food science ,education ,0303 health sciences ,education.field_of_study ,biology ,030306 microbiology ,Lactococcus lactis ,Antimicrobial ,biology.organism_classification ,Lactic acid ,Sodium caseinate ,Biochemistry ,chemistry ,Listeria ,Aqueous two-phase system ,Sodium alginate ,Entrapment ,Bacteria ,Food Science ,Biotechnology - Abstract
In order to control undesirable microorganisms growth in foods, the performance of alginate and alginate-caseinate (an aqueous two-phase system) matrices entrapping lactic acid bacteria (LAB) (Lactobacillus paracasei LAB1 and Lactococcus lactis LAB3) was investigated. Polymeric matrices were initially loaded with Lcells at similar to 10(8-10) or similar to 10(4-6) CFU mL(-1), and were monitored, in liquid and gelled form (beads), for 12 days at 30 degrees C. In the liquid form, maximum cell density (similar to 10(9) CFU mL(-1)) was reached after 24 h whatever the matrix. Then, the Lpopulation decreased but remained higher in alginate-caseinate matrices: 10(7) and 10(6) CFU mL(-1) of LAB3 cells were enumerated after 12 days in alginate-caseinate and in alginate matrices, respectively. Anti-Listeria activity (assayed by agar well diffusion method) did not vary much over 12 days and was also higher for cells entrapped in alginate-caseinate matrices. When matrices were gelled, similar trends were observed: at "Day 12", LAB3 population was 10(4-5) and 10(2-3) CFU/bead, and, LAB1 population was 10(5-6) and 10(3-4) CFU/bead, in alginate-caseinate and alginate beads, respectively. Antimicrobial activity of alginate-caseinate beads containing LAB1 cells was quite constant over 12 days. The anti-Listeria activity of Lcell-free supernatants incorporated in matrices with caseinate was also higher. In fact, the presence of caseinate was shown to promote both the survival of Lcells and the release of their antimicrobial metabolites. Observation of liquid and gelled matrices by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) revealed a preferential localization of Lcells in casein-rich microdomains which could affect favorably the efficiency of bipolymeric matrices. (C) 2014 Elsevier Ltd. All rights reserved.
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- 2015
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