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With increased awareness among consumers regarding food safety and security, food allergen control has become an indispensable requirement in the food industry. Although several methods for detecting allergens in food products are available, highly sensitive techniques are required. In this study, we developed a technique named as peptide array-based inhibition enzyme-linked immunosorbent assay (ELISA), Pep-iEIA, for evaluating antigenicity and detecting cow's milk antigen in infant formula products, using a peptide array consisting of a series of overlapping peptides found in allergenic milk proteins. Pep-iEIA was used to examine five cow's milk-based infant formulas with different degrees of hydrolyzation, and the assay offered both more sensitive detection and detailed analysis of remaining antigenic peptides in allergen compared to conventional ELISA. The antigenicity level of the allergenic peptides identified using Pep-iEIA was confirmed by surface plasmon resonance assay. We believe that Pep-iEIA will be highly useful for antigenicity evaluation of dairy products consumed by infants and patients with cow's milk allergy.

cited By 0; International audience; 2-layer laminated sheets (PE/18-8) with Polyethylene (PE) and austenitic 18-8 stainless steels (18-8) were prepared by a new adhesion method, a double-step treatment consisting of applying low dose (ℜ 0.43MGy) homogeneous low energy electron beam irradiation (HLEBI) prior to hot-press under 5MPa and 343 K. Although the weak hot-press adhesion of the PE/18-8 was observed without HLEBI, the new adhesion raised the bonding forces as evidenced by the mean adhesive forces of peeling resistance (oFp). Based on the 3-parameter Weibull equation, the lowest oFp value at peeling probability (Pp) of zero (Fs) could be estimated. An increasing trend in Fs occurred by the double-step treatment applying HLEBI up to 0.30MGy reaching a maximum at 0.85Nm-1, improving the safety level without radiation damage. When HLEBI cut the chemical bonds in PE polymer and generated terminated atoms with dangling bonds, they probably induced the chemical bonding. Therefore, increasing adhesion force between the laminated sheets could be explained. © 2016 The Japan Institute of Metals and Materials.

Effects of homogeneous low voltage electron beam irradiation (HLEBI) without glue on the adhesive force of tensile shear strength (¸B) at each shear probability (Ps) of laminated PDMS/PTFE sheets of polydimethylsiloxane (PDMS) and Polytetrafluoroethylene (PTFE) were investigated. AlthoughB of shear at low and mid Ps of 0.06 and 0.50 were 0.015 and 0.04MPa before treatment, 0.22MGy-HLEBI enhanced theB up to the largest values of 0.098 and 0.11MPa of the laminated sheets, respectively. They were more than 6 and 3 times larger than those before treatment. On the other hand, additional HLEBI apparently reduced theB of laminated sheets irradiated at more than 0.43MGy as usual radiation damages. The effects of HLEBI on adhesion force ofB can be explained by the enhancement of cross-linking at PDMS/PTFE interface. When HLEBI cut the chemical bonds and generated active terminated atoms with dangling bonds at cross-linked PDMS and PTFE polymers, strengthening the bonding force of cross-linking polymers was induced by the chemical bonding and intermolecular attractive force. (doi:10.2320/matertrans.MBW201411)