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20 results on '"Chen, Zuxian"'

5. Duck TRIM29 negatively regulates type I IFN production by targeting MAVS

Author

Li, Weiqiang, primary, Song, Yating, additional, Du, Yuqing, additional, Huang, Zhanhong, additional, Zhang, Meng, additional, Chen, Zuxian, additional, He, Zhuoliang, additional, Ding, Yangbao, additional, Zhang, Junsheng, additional, Zhao, Luxiang, additional, Sun, Hailiang, additional, and Jiao, Peirong, additional

Published
2023
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7. Layered Double Hydroxide Modified with Deoxycholic and Hyaluronic Acids for Efficient Oral Insulin Absorption

Author

Huang,Xia, Han,Shangcong, Chen,Zuxian, Zhao,Lei, Wang,Changduo, Guo,Qingyang, Li,Yanfeng, Sun,Yong, Huang,Xia, Han,Shangcong, Chen,Zuxian, Zhao,Lei, Wang,Changduo, Guo,Qingyang, Li,Yanfeng, and Sun,Yong

Abstract

Xia Huang, 1,* Shangcong Han, 1,* Zuxian Chen, 1 Lei Zhao, 2 Changduo Wang, 1 Qingyang Guo, 3 Yanfeng Li, 1 Yong Sun 1 1Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao, People’s Republic of China; 2Lunan Better Pharmaceutical Co., Ltd, Linyi, People’s Republic of China; 3College of Fisheries, Henan Normal University, Xinxiang, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yong Sun Email sunyong@qdu.edu.cnIntroduction: This study aimed to construct a layered double hydroxide (LDH) nanoparticle delivery system that was modified by deoxycholic acid (DCA) and hyaluronic acid (HA) to increase the bioavailability of oral insulin.Methods: LDH-DCA-HA was synthesized by the hybridization of DCA and HA with LDH. Subsequently, insulin was loaded onto LDH-DCA-HA, resulting in the formation of INS@LDH-DCA-HA. The in vivo and in vitro mechanisms of insulin release, as well as the efficiency of insulin absorption, were analyzed before and after DCA-HA modification.Results: MTT assay showed that there was satisfactory biocompatibility between LDH-DCA-HA and Caco-2 cells at a concentration below 1000 μg/mL. Flow cytometry analysis revealed that Caco-2 cells absorbed INS@LDH-DCA-HA more readily than insulin. Measurement of transepithelial electrical resistance indicated that INS@LDH-DCA-HA induced the reversible opening of tight cell junctions, thereby facilitating its absorption. This was confirmed via laser confocal microscopy analysis, revealing that a large amount of zonula occludens-1 tight junction (TJ) protein was utilized for the paracellular pathway of nanoparticles. We also measured the blood glucose levels of type I diabetic mice and found that oral INS@LDH-DCA-HA exerted a steady hypoglycemic effect lasting 12 h, with a small range of postprandial blood glucose fluctuation. Immunofluorescence analysis showed that the strong penetration ability of INS@LDH-DCA-HA all

Published
2021

8. Overcoming Multiple Absorption Barrier for Insulin Oral Delivery Using Multifunctional Nanoparticles Based on Chitosan Derivatives and Hyaluronic Acid

Author

Chen,Zuxian, Han,Shangcong, Yang,Xiaotang, Xu,Lisa, Qi,Hong, Hao,Guizhou, Cao,Jie, Liang,Yan, Ma,Qingming, Zhang,Guimin, Sun,Yong, Chen,Zuxian, Han,Shangcong, Yang,Xiaotang, Xu,Lisa, Qi,Hong, Hao,Guizhou, Cao,Jie, Liang,Yan, Ma,Qingming, Zhang,Guimin, and Sun,Yong

Abstract

Zuxian Chen,1,* Shangcong Han,1,* Xiaotang Yang,1 Lisa Xu,1 Hong Qi,2 Guizhou Hao,3 Jie Cao,1 Yan Liang,1 Qingming Ma,1 Guimin Zhang,3 Yong Sun1 1Department of Pharmaceutics, School of Pharmacy, Qingdao University, Qingdao 266021, People’s Republic of China; 2Department of General Surgery, Qingdao Municipal Hospital, Qingdao 266071, People’s Republic of China; 3Department of Scientific Research, Lunan Pharmaceutical Corporation, Linyi 276001, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yong Sun Tel +86-532-82991203Email sunyong@qdu.edu.cnBackground: Although dynamics and uses of modified nanoparticles (NPs) as orally administered macromolecular drugs have been researched for many years, measures of molecule stability and aspects related to important transport-related mechanisms which have been assessed in vivo remain as relatively under characterized. Thus, our aim was to develop a novel type of oral-based delivery system for insulin and to overcome barriers to studying the stability, transport mechanisms, and efficacy in vivo of the delivery system.Methods: NPs we developed and tested were composed of insulin (INS), dicyandiamide-modified chitosan (DCDA-CS), cell-penetrating octaarginine (r8), and hydrophilic hyaluronic acid (HA) and were physically constructed by electrostatic self-assembly techniques.Results: Compared to free-insulin, levels of HA-DCDA-CS-r8-INS NPs were retained at more desirable measures of biological activity in our study. Further, our assessments of the mechanisms for NPs suggested that there were high measures of cellular uptake that mainly achieved through active transport via lipid rafts and the macropinocytosis pathway. Furthermore, investigations of NPs indicated their involvement in caveolae-mediated transport and in the DCDA-CS-mediated paracellular pathway, which contributed to increasing the efficiency of sequential transportation from the apical to basolatera

Published
2020

10. Pathogenicity of different H5N6 highly pathogenic avian influenza virus strains and host immune responses in chickens

Author

Chen, Zuxian, primary, Wang, Zhenyu, additional, Zhao, Xiya, additional, Guan, Yun, additional, Xue, Qian, additional, Li, Jinrong, additional, Liu, Zhiting, additional, Zhao, Bingbing, additional, He, Zhuoliang, additional, Huang, Jianni, additional, Liao, Ming, additional, Song, Yafen, additional, and Jiao, Peirong, additional

Published
2020
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13. Genetic characteristics, pathogenicity and transmission of H5N6 highly pathogenic avian influenza viruses in Southern China

Author

Qu, Nannan, primary, Zhao, Bingbing, additional, Chen, Zuxian, additional, He, Zhuoliang, additional, Li, Weiqiang, additional, Liu, Zhiting, additional, Wang, Xia, additional, Huang, Jianni, additional, Zhang, You, additional, He, Wanting, additional, Sun, Jie, additional, Qin, Zhifeng, additional, Liao, Ming, additional, and Jiao, Peirong, additional

Published
2019
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19. Avian Influenza (H7N9) Viruses Co-circulating among Chickens, Southern China.

Author

Nianchen Wang, Minhua Sun, Wenqing Wang, Guowen Ouyang, Zuxian Chen, You Zhang, Bingbing Zhao, Siyu Wu, Jianni Huang, Hailiang Sun, Ming Liao, Peirong Jiao, Wang, Nianchen, Sun, Minhua, Wang, Wenqing, Ouyang, Guowen, Chen, Zuxian, Zhang, You, Zhao, Bingbing, and Wu, Siyu

Subjects
H7N9 Influenza, AVIAN influenza, INFLUENZA A virus, CHICKEN diseases, HEMAGGLUTININ, PROTEIN metabolism, CLASSIFICATION of viruses, AVIAN influenza epidemiology, AMINO acids, EPIDEMIOLOGY, BIOLOGICAL evolution, GENE expression, METABOLISM, POULTRY, PROTEINS, SURVIVAL analysis (Biometry), MICROBIAL virulence, VIRUSES
Abstract

In April 2017, three avian influenza (H7N9) viruses were isolated from chickens in southern China. Each virus had different insertion points in the cleavage site of the hemagglutinin protein compared to the first identified H7N9 virus. We determined that these viruses were double or triple reassortant viruses. [ABSTRACT FROM AUTHOR]

Published
2017
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20. RNF216 Inhibits the Replication of H5N1 Avian Influenza Virus and Regulates the RIG-I Signaling Pathway in Ducks.

Author

He Z, Li W, Zhang M, Huang M, Chen Z, Zhao X, Ding Y, Zhang J, Zhao L, and Jiao P

Subjects
Animals, Fibroblasts immunology, Fibroblasts virology, Avian Proteins immunology, Avian Proteins genetics, Avian Proteins metabolism, Ubiquitination, DEAD Box Protein 58 metabolism, DEAD Box Protein 58 immunology, Ducks immunology, Ducks virology, Virus Replication immunology, Signal Transduction immunology, Influenza in Birds immunology, Influenza in Birds virology, Influenza A Virus, H5N1 Subtype immunology, Influenza A Virus, H5N1 Subtype physiology, Immunity, Innate immunology, Ubiquitin-Protein Ligases genetics, Ubiquitin-Protein Ligases immunology
Abstract

The RING finger (RNF) family, a group of E3 ubiquitin ligases, plays multiple essential roles in the regulation of innate immunity and resistance to viral infection in mammals. However, it is still unclear whether RNF proteins affect the production of IFN-I and the replication of avian influenza virus (AIV) in ducks. In this article, we found that duck RNF216 (duRNF216) inhibited the duRIG-I signaling pathway. Conversely, duRNF216 deficiency enhanced innate immune responses in duck embryonic fibroblasts. duRNF216 did not interacted with duRIG-I, duMDA5, duMAVS, duSTING, duTBK1, or duIRF7 in the duck RIG-I pathway. However, duRNF216 targeted duTRAF3 and inhibited duMAVS in the recruitment of duTRAF3 in a dose-dependent manner. duRNF216 catalyzed K48-linked polyubiquitination of duck TRAF3, which was degraded by the proteasome pathway. Additionally, AIV PB1 protein competed with duTRAF3 for binding to duRNF216 to reduce degradation of TRAF3 by proteasomes in the cytoplasm, thereby slightly weakening duRNF216-mediated downregulation of IFN-I. Moreover, although duRNF216 downregulated the IFN-β expression during virus infection, the expression level of IFN-β in AIV-infected duck embryonic fibroblasts overexpressing duRNF216 was still higher than that in uninfected cells, which would hinder the viral replication. During AIV infection, duRNF216 protein targeted the core protein PB1 of viral polymerase to hinder viral polymerase activity and viral RNA synthesis in the nucleus, ultimately strongly restricting viral replication. Thus, our study reveals a new mechanism by which duRNF216 downregulates innate immunity and inhibits AIV replication in ducks. These findings broaden our understanding of the mechanisms by which the duRNF216 protein affects AIV replication in ducks., (Copyright © 2024 by The American Association of Immunologists, Inc.)

Published
2024
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