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The Carboniferous strata in the northeastern Junggar Basin are an important exploration field for natural gas in the basin. However, volcanic rocks have long been the primary exploration target. In contrast, the exploration and research of clastic rocks associated with source formations have been largely overlooked, resulting in an insufficient understanding of the reservoir forming conditions and exploration potential of Carboniferous clastic rocks. Through the evaluation of Carboniferous source rocks, effective source stove characterization, clastic reservoir evaluation, oil and gas source correlation, and reservoir formation model construction in this region, three key findings have been made. First, the Carboniferous in the northeastern Junggar Basin has developed three sets of high-quality gas source rocks: the Dishuiquan Formation, the Songkalsu B Member, and the Shiqiantan Formation. These formations correspond to three hydrocarbon source centers: the Sannan–Dishuiquan Sag, the Wucaiwan Sag–Dajing area, and the Dongdao Haizi Sag–Baijiahai High. Second, the Carboniferous system in the northeast has developed multiple types of large-scale reservoirs, including sand conglomerates, sandstones, turbidites, dolomitic rocks, and shale. These reservoirs are generally characterized by low porosity to ultra-low porosity and low permeability to ultra-low permeability reservoirs. There is a dissolution pore development zone at depths of 2900–4500 m. Third, a comparison of oil and gas sources reveals that all three sets of gas source rocks contribute to the natural gas found in the northeast, with obvious characteristics of near-source reservoir formation. The Carboniferous clastic rocks host two types of natural gas reservoirs: unconventional and conventional near-source reservoirs. It is predicted that there is an orderly accumulation pattern of shale gas, tight sandstone gas, and conventional natural gas reservoirs. This study reveals that the Carboniferous clastic rock source and reservoir configuration in the northeastern Junggar Basin is highly favorable, and the natural gas reservoirs in source and near-source clastic rocks represent important exploration directions.

Abstract Background Bisphosphonates (BPs) are widely used to inhibit excessive osteoclast activity. However, the potential to compromise bone defect healing has limited their broader application. To better understand the influence of BPs on bone regeneration, we established a bone grafting model with Zoledronate administration, aiming to deepen the understanding of bone remodeling and mineralization processes. Methods A bone grafting model was established in the distal femurs of male Sprague–Dawley rats. The experimental group received systemic administration of Zoledronate (ZOL, 0.2 mg/kg, administered twice). Histological analysis and immunohistochemistry (IHC) were employed to assess osteoblastic and macrophage activity, tartrate-resistant acid phosphatase (TRAP) staining was used to evaluate osteoclastogenesis. Mineralization was assessed through Micro-CT analysis, Raman spectroscopy, and back-scatter scanning electron microscopy (BSE-SEM). Additionally, the in vitro effects of ZOL on osteoblast and osteoclast activity were investigated to further elucidate its impact on bone regeneration. Results In vivo, the ZOL group showed increased bone mass, as observed in histological and radiological assessments. However, Micro-CT, Raman spectroscopy, and BSE-SEM detection revealed lower mineralization levels in ZOL group's regenerated bone. Acid-etched SEM analysis showed abnormal osteocyte characteristics in ZOL-group’s regenerated bone. Simultaneously, elevated osteopontin (OPN), F4/80 expression along with reduced TRAP expressing was found in the grafting region of ZOL group. In vitro, ZOL did not negatively impact osteogenetic activity (ALP, BMP4, OCN expression) at the tested concentrations (0.02–0.5 g/ml) but significantly impaired mineralization and inhibited osteoclast formation, even at the lowest concentration. Conclusions This study highlights a less recognized negative effect of ZOL on bone mineralization during bone regeneration. More research is needed to elucidate the underlying mechanism.

Taking the deep-ultra deep glutenite reservoirs in the Upper Wuerhe Formation of Permian in Fukang Sag and Dongdaohaizi Sag as the research object and representative of the eastern Junggar Basin, its characteristics and main controlling factors are analyzed using various methods, including core analysis, thin section examination, and scanning electron microscope observation. High-pressure mercury injection and logging imaging were also employed to reveal the reservoir that Fukang and Dongdaohaizi sags harbor deep-ultra deep glutenite reservoirs, with Fukang Sag being a typical low porosity to ultra-low permeability reservoir, while Dongdaohaizi Sag is a low porosity to low permeability reservoir. Reservoir space types vary between the two sags, with Fukang Sag characterized by microfractures and corrosion pores, while a large number of corrosion pores with fewer fractures existing in Dongdaohaizi Sag. Both compaction and cementation exhibit a strong destructive effect on the reservoirs in the eastern Junggar Basin. However, the compaction effect in Fukang Sag is very strong, and the dissolution effect is weak. The large number of fractures generated by overpressure becomes an effective channel for ultra-deep oil and gas migration. The cracks in the Dongdaohaizi Sag are underdeveloped, and a large number of intra-particle corrosion pores generated by the dissolution of feldspar and turbidite improve reservoir properties. In addition, its rich turbidite also plays a compressive and pore retention role. There are two types of reservoir models developed in the Upper Wuerhe Formation of Permian in the eastern Junggar Basin: the deep fracture model represented by the Fukang Sag and the solution pore model rich in turbidite in Dongdaohaizi Sag. These models create favorable conditions for oil and gas accumulation in the deep-ultra deep reservoirs in the depression area.

The improper treatment of crop straw not only leads to resource wastage but also adversely impacts the ecological environment. However, the application of microorganisms can accelerate the decomposition of crop straw and improve its utilization. In this study, cellulose-degrading microbial strains were isolated from naturally decayed corn straw and screened using Congo red staining, along with assessing variations in carboxymethyl cellulase (CMCase) activity, filter paper enzyme (FPase) activity and β-glucosidase (β-Gase) activity, as well as the degradation rate. The eight strains, namely Neurospora intermedia isolate 29 (A1), Streptomyces isolate FFJC33 (A2), Gibberella moniliformis isolate FKCB-009 (A3), Fusarium fujikuroi isolate EFS3(2) (A4), Fusarium Fujikuroi isolate FZ04 (A5), Lysine bacillus macroides strain LNHL43 (B1), Bacillus subtilis strain MPF30 (B2) and Paenibacilli lautus strain ALEB-P1 (C), were identified and selected for microbial strain consortium design based on their high activities of CMCase, FPase and β-Gase. The fungi, bacteria and actinomycete strains were combined without antagonistic effects on corn straw decomposition. The results showed the A2B2 combination had a significantly higher FPase at 55.44 U/mL and β-Gase at 25.73 U/mL than the other two strain combinations (p < 0.05). Additionally, the degradation rate of this combination was 40.33%, which was considerably higher than that of the other strains/consortia. The strain combination A4B2C also had superior enzyme activity, including CMCase with a value of 35.03 U/mL, FPase with a value of 63.59 U/mL and β-Gase with a value of 26.15 U/mL, which were significantly different to those of the other three strain combinations (p < 0.05). Furthermore, seven single microbial strains with high cellulase activities were selected to construct various microbial consortiums for in situ composting in order to evaluate their potential. Taken as a whole, the results of composting, including temperature, moisture content, pH, E4/E6 value and seed germination index, indicated that the microbial strain consortium consisting of Neurospora intermediate isolate 29, Fusarium fujikuroi isolate EFS3(2), Fusarium fujikuroi isolate FZ04, Lysinibacillus macrolides, Lysinibacillus sphaericus, Bacillus subtilis and Paenibacillus lautus was advantageous for corn straw decomposition and yielded high-quality compost. The screened flora was able to effectively degrade corn straw. This study provides a novel solution for the construction of a microbial consortium for the composting of corn straw.

As the largest hydrocarbon-rich sag in the Junggar Basin, the limited research on geochemical characteristics, sedimentary environment and bio-precursors of source rocks in the Fukang Sag has seriously restricted the understanding of the poor proven oil and gas resources in the sag. A combined geological and geochemical analysis on the Lucaogou Formation source rocks in the Fukang Sag was conducted based on the experimental analysis results of the newly drilled source rock samples on the Fudong Slope. The results show that the Lucaogou Formation source rocks in the Fukang Sag generally belong to source rocks of medium to very good quality, with organic matter of type Ⅱ to type Ⅲ, and mainly in the thermal maturity of early mature to mature stage. Biomarker characteristics show that the organic matter of Lucaogou Formation source rocks in the Fukang Sag is a mixture of terrestrial higher plants and aquatic algae and bacteria and maybe some Dunaliella-like green algae, which is generally deposited in the anoxic to oxic, fresh to brackish, intermittent stratified water environment, showing obvious mudstone sedimentary characteristics. The geochemical characteristics of the Lucaogou Formation source rocks in the Fukang Sag vary greatly on the plane, among which, the central and northern subsags show great hydrocarbon generation potential.

Due to the widespread use of intensive cropping patterns, the problem of continuous cropping obstacle, which is dominated by autotoxicity, has been becoming more and more prominent. Although many methods have been proposed to overcome the continuous cropping obstacle of cucumber, no study has reported the screening and evaluation of cucumber germplasm resistant to autotoxicity. In this study, 28 physiological indices related to the cucumber bud stage under cinnamic acid (CA) treatment were determined. In total, 45 cucumber cultivars were classified into three groups using principal component analysis and cluster analysis, and a model for evaluating cucumber resistance to autotoxicity was developed. The evaluation model was validated using autotoxicity-tolerant and non-autotoxicity-tolerant cultivars. The results showed that the growth of non-autotoxicity-tolerant cultivars was significantly inhibited compared to autotoxicity-tolerant cultivars. This indicated that the evaluation model of cucumber autotoxicity tolerance is reliable. The results of this study provide a valuable reference for the application of cucumber autotoxicity-tolerant germplasm resources and the development of autotoxicity-tolerant genes.

Abstract Background While autophagy is essential for stem cells’ self-renewal and differentiation, its effect on bone marrow mesenchymal stem cells (BMSCs) remains unclear. This study aimed to investigate the interaction between autophagy and osteogenic differentiation using rapamycin (RAPA), a classical autophagy agonist with osteo-regulatory effects. Methods Rat BMSC’s autophagy was analyzed after osteoinduction (0, 7, 14, and 21 d) by western blotting, immunofluorescence, and real-time quantitative polymerase chain reaction (RT-qPCR). In addition, we evaluated osteogenic differentiation using alizarin red staining, alkaline phosphatase assays, and RT-qPCR/Western blotting quantification of bone sialoprotein, type 1 collagen, alkaline phosphatase, osteopontin, and Runt-related transcription factor 2 mRNA and protein levels. Results The BMSC’s basal autophagy level gradually decreased during osteogenic differentiation with a decrease in BECN1 level and the lipidated (LC3-II) to unlipidated (LC3-I) microtubule-associated protein 1 light chain 3 ratio and an increase in the expression of selective autophagic target p62. In contrast, it increased with increasing RAPA concentration. Furthermore, while 2 nM RAPA promoted BMSC osteogenic differentiation on days 7 and 14, 5 nM RAPA inhibited osteogenesis on days 14 and 21. Inhibition of autophagy by the inhibitor 3-methyladenine could impair RAPA’s osteogenesis-enhancing effect on BMSCs. Conclusions The BMSC’s basal autophagy level decreased over time during osteogenic differentiation. However, an appropriate RAPA concentration promoted BMSC osteogenic differentiation via autophagy activation.

Abstract Introduction The aim of this study was to verify whether the use of short implants could optimize stress distribution of bone surrounding implants in atrophic mandibles with different bone qualities. Methods A three-dimensional model of the atrophic mandible with three levels of bone quality was made using computer software. Short implants (6 mm) and standard implants (10 mm) were used in four designs: Design 1 "All-On four", Design 2 "All-On-four" with two short implants, Design 3 four vertical implants with two short implants, and Design 4 six short implants. The distal short implants were placed at the first molar position. All twelve models were imported into finite element analysis software, and 110 N oblique force was loaded on the left second premolar. Maximum principal stress values of peri-implant bone and the volumes of bone with over 3000 microstrians (overload)were analyzed. Result Stress values and volumes of overload bone increased in all four groups with the decline of bone quality. The highest stress values were found in the cortical bone surrounding the Design 1 inclined implant in two lower bone quality mandibles, and the lowest in Design 3. However, Design 1 had less overload bone tissue than all three designs with short implants. Conclusion Short implants placed posteriorly helped decrease stress values in peri-implant bone, while bone surrounding short implants had a high resorption risk in low bone quality mandible.

In this paper, the lithology, pore type, throat structure, and physical characteristics of the sandstone and conglomerate reservoirs of the Upper Permian Shangwuerhe Formation in the Fukang Sag of the Junggar Basin were analyzed through rock cast thin section, scanning electron microscopy, fluid inclusions, piezometric mercury, and porosity–permeability analysis. In addition, the reservoir densification mechanism and the genesis of deep effective reservoirs were discussed. The results show that the reservoir is dominated by lithic sandstone (or lithic sandstone conglomerate). The lithic fragments primarily comprise tuffaceous volcanic rocks, supported by grains and cemented by clay, carbonate, authigenic quartz, and laumontite. The reservoir properties are characterized by extra-low porosity and permeability, and the pore type is dominated by inter- and intragrain dissolved pores of lithic fragments, feldspar, and quartz. The pore connectivity is poor due to poorly sorted extra-fine throat channels. The tightness of the reservoir.is due to the strong cementation of calcite, chlorite, montmorillonite, illite-montmorillonite mixed layer, authigenic quartz, and laumontite. Furthermore, the large amount of Ca2+ released by the hydration of tuff rock debris and intermediate–basic volcanic rock debris and the CO2-rich thermal fluid from the deep layers cause the development of several calcites. The formation of several montmorillonites is mainly related to the alteration of filled volcanic ash, and the hydration of volcanic tuff material primarily causes the development of laumontite cementation. The dissolution of feldspar and various volcanic lithic fragments by acidic fluids triggers the physical improvement of the reservoir in the local section.

As an unconventional petroleum reservoir, the volcanic reservoir is essential for finding large-scale oil and gas reserves in deep sedimentary basins. Based on basin modeling, organic geochemistry, and organic petrology, this study evaluates the exploration potential of the Carboniferous volcanic-petroleum system in the Jimsar Sag of the southeastern Junggar Basin. The Carboniferous source rocks in the study area were developed in the Lower Carboniferous Songkharsu Formation, a set of marine–terrigenous transitional source rocks, lithologically composed of carbonaceous mudstone, mudstone, and coal. The kerogen is characterized by type II2–III, indicating a gas-prone source rock. Carbonaceous mudstones, mudstone, and coal are classified as medium–good, medium, and poor source rocks, respectively. The hydrocarbon-generating potential is close to that of the Kelameili gas field. The Carboniferous-reservoir oil in well J15 is characterized by 13C-enriched stable carbon isotopes, relatively high ƩC31–35/C30 and Pr/Ph ratios, relatively low density, gammacerane index, regular C29-sterane content, and undetected β-carotene, indicating a Songkharsu origin. The Carboniferous-reservoired gas from well J3301 has stable carbon isotope composition enriched in 13C, similar to the gas from the Kelameili gas field, and should be from the Songkharsu source rock. This set of source rocks is widely distributed in the Jimsar Sag with considerable thickness, among which thickness >100 m reaches 580 km2, accounting for 38.7% of the whole sag. The Songkharsu source rocks entered the main oil-generating window at the end of the Early Jurassic. The area of Songkharsu source rocks in the Jimsar Sag with gas-generating intensity greater than 20 × 108 m3/km2 reaches 1,015 km2, whereas that with oil-generating intensity greater than 500 × 104 t/km2 reaches 1,146 km2. However, 13 Carboniferous volcanic lithological traps were found in the sag, covering an area of 230 km2. The Carboniferous volcanic-petroleum system in the Jimsar Sag has the resource potential to form large and medium oil and gas fields, which could become a critical replacement field for volcanic oil and gas exploration in the Junggar Basin after the Klameili gas field.

Oil shale is a kind of unconventional energy resource with abundant reserves, but its exploitation has a continuous negative impact on the environment, which has hindered the research and exploitation of oil shale under the international environmental consensus on issues such as climate change. Therefore, more attention should be paid to environmental problems as the side effect of oil shale exploitation. With the combination of field research, literature collection, and tracking survey, the oil shale open-pit exploitation and management process in Maoming, Guangdong, China, has been investigated, and its development and transformation model has been subsequently refined and summarized. The research results show that Maoming oil shale open-pit mine area has gone through four main stages: pre-exploitation stage, large-scale utilization stage, restoration stage, and green development stage. Through the management of mine pit treatment, vegetation restoration, ecological park construction, and tourism resource development, the abandoned open-pit mine has been transformed into an ecological park combining ecosystem, tourism, and cultural resources. In this process, this area has achieved the transformation from rough resource extraction to environment-friendly sustainable growth in its development mode. As a successful case of open-pit mine management in the world, the ecological restoration experience in Maoming can function as a reference for the smooth development and transformation of other oil shale mines in developing countries.

Worldwide, scarce water resources and substantial food demands require efficient water use and high yield. This study investigated whether irrigation frequency can be used to adjust soil moisture to increase grain yield and water use efficiency (WUE) of high-yield maize under conditions of mulching and drip irrigation. A field experiment was conducted using three irrigation intervals in 2016: 6, 9, and 12 days (labeled D6, D9, and D12) and five irrigation intervals in 2017: 3, 6, 9, 12, and 15 days (D3, D6, D9, D12, and D15). In Xinjiang, an optimal irrigation quota is 540 mm for high-yield maize. The D3, D6, D9, D12, and D15 irrigation intervals gave grain yields of 19.7, 19.1–21.0, 18.8–20.0, 18.2–19.2, and 17.2 Mg ha−1 and a WUE of 2.48, 2.53–2.80, 2.47–2.63, 2.34–2.45, and 2.08 kg m−3, respectively. Treatment D6 led to the highest soil water storage, but evapotranspiration and soil-water evaporation were lower than other treatments. These results show that irrigation interval D6 can help maintain a favorable soil-moisture environment in the upper-60-cm soil layer, reduce soil-water evaporation and evapotranspiration, and produce the highest yield and WUE. In this arid region and in other regions with similar soil and climate conditions, a similar irrigation interval would thus be beneficial for adjusting soil moisture to increase maize yield and WUE under conditions of mulching and drip irrigation. Keywords: Irrigation frequency, Soil moisture, Maize, High yield (>15 Mg ha−1), Water use efficiency

Although the Fukang Sub-depression is the largest hydrocarbon generation depression in the Junggar Basin and promises favorable potential for oil and gas exploration, only several smallâmedium oil/gas accumulations have been discovered so far. After analyzing the molecular and stable carbon isotopic compositions of natural gases and its associated low molecular weight hydrocarbons (LMWHs) from eastern Fukang Sub-depression, their genetic types and origins are discussed in this study. Natural gases are wet gases with an average dryness coefficient of 0.84. Stable carbon isotopes of methane and ethane are well correlated with the thermal evolution trend of coal-derived gases. The C5â7 LMWHs are enriched in methyl cyclohexane but they lack n-alkanes, indicating a predominance of higher plant input. Gases have maturities ranging 0.76%â0.93%Ro and are well consistent with their low n-heptane (H) and iso-heptane (I) contents. The geochemical characteristics of natural gases from eastern Fukang Sub-depression are quite similar with those from the TurpanâHami Basin. Their gases are considered to be low-mature gases derived from the LowerâMiddle Jurassic coal measures. In contrast, they show remarkable differences with those from the Wucaiwan gas field; they are considered to have high-mature gases derived from the Carboniferous coal measures. Keywords: Coal-derived gas, Stable carbon isotopes, Low molecular weight hydrocarbons, Fukang sub-depression, Junggar Basin, MiddleâLower Jurassic

Based on observation of cores and casting thin sections, reservoir fluorescence, hydrocarbon geochemical characteristics and oil production test data, the factors controlling the tight oil accumulation and high production in the Da'anzhai Member in the Jurassic Ziliujing Formation in central Sichuan Basin are studied. The shelly limestone, muddy shell limestone and tight limestone are all oil-bearing in the Da'anzhai Member. The dissolved pores and fractures in shelly limestone and the matrix pores (microfractures, intercrystal pores) in tight limestone are all oil-bearing in large area, which is the reason why oil wells can keep low production for a long period of time. On the basis of the extensive oil-bearing, natural gas from the Upper Triassic Xujiahe Formation provided the major power for oil migration and accumulation in the Da'anzhai low amplitude, water-free tight limestone reservoirs, driving the dispersed oil from the matrix pores into an accumulation, so the oil is more enriched in the western structures of central Sichuan Basin where oil wells are higher in gas-oil ratio. In contrast, in the east of central Sichuan, the Huayingshan major faults generally cut through Da'anzhai Member, allowing gas to migrate to the formations above Da'anzhai Member along fractures to accumulate or dissipate, without gas as driving force, as a result, the oil is less enriched than that in the west part of central Sichuan. Key words: tight oil, central Sichuan Basin, Jurassic Da'anzhai Member, enrichment and high production, gas drive, fault

Proteolysis-targeting chimera (PROTAC) that specifically targets harmful proteins for destruction by hijacking the ubiquitin–proteasome system is emerging as a potent anticancer strategy. How to efficiently modulate the target degradation remains a challenging issue. In this study, we employ a single amino acid–based PROTAC, which uses the shortest degradation signal sequence as the ligand of the N-end rule E3 ubiquitin ligases to degrade the fusion protein BCR (breakpoint cluster region)–ABL (Abelson proto-oncogene), an oncogenic kinase that drives the progression of chronic myeloid leukemia. We find that the reduction level of BCR–ABL can be easily adjusted by substituting different amino acids. Furthermore, a single PEG linker is found to achieve the best proteolytic effect. Our efforts have resulted in effective degradation of BCR–ABL protein by the N-end rule pathway and efficient growth inhibition of K562 cells expressing BCR–ABL in vitro and blunted tumor growth in a K562 xenograft tumor model in vivo. The PROTAC presented has unique advantages including lower effective concentration, smaller molecular size, and modular degradation rate. Demonstrating the efficacy of the N-end rule–based PROTACs in vitro and in vivo, our study further expands the limited degradation pathways currently available for PROTACs in vivo and is easily adapted for broader applications in targeted protein degradation. [ABSTRACT FROM AUTHOR]