1. Detection of IncN‐pST15 one‐health plasmid harbouring bla KPC‐2 in a hypermucoviscous Klebsiella pneumoniae CG258 isolated from an infected dog, Brazil
- Author
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Sibele Konno, Mariana H Cappellanes, Nilton Lincopan, Fernanda Esposito, Louise Cerdeira, Bruna Fuga, Marcia Beserra Xavier Cortez, Herrison Fontana, César M de Souza, Fábio P. Sellera, Carla Berl, Marcelo Ikeda, and Brenda Cardoso
- Subjects
clone (Java method) ,0303 health sciences ,General Veterinary ,General Immunology and Microbiology ,040301 veterinary sciences ,Transmission (medicine) ,Klebsiella pneumoniae ,04 agricultural and veterinary sciences ,General Medicine ,Biology ,biology.organism_classification ,Microbiology ,0403 veterinary science ,03 medical and health sciences ,Plasmid ,One Health ,Plasmidome ,SEQUENCIAMENTO GENÉTICO ,Gene ,Bacteria ,030304 developmental biology - Abstract
The emergence and rapid spread of carbapenemase-producing Enterobacterales represents a serious public health concern. Critically, these global priority bacteria have begun to be reported in companion animals, implying a potential risk of cross-transmission between humans and pets. Using long-read (MinION) and short-read (Illumina) sequencing technologies, we have identified and characterized a hypermucoviscous KPC-2-producing Klebsiella pneumoniae strain belonging to the high-risk international clone ST11/CG258, in a dog with urinary tract infection. Strikingly, the blaKPC-2 gene was carried by a 54-kb IncN plasmid assignated to ST15, which shared 99.8 and 96.8% pairwise identity with IncN-pST15 plasmids from human and environmental K. pneumoniae strains, respectively; all come from an area with high endemicity of KPC-2. Our findings suggest that IncN-pST15 plasmids conferring carbapenem resistance can play as important a role as clonal transmission of K. pneumoniae, representing another major challenge for One Health.
- Published
- 2021
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