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Changlin Mao,1 Wei Dong,2 Jiaju Lu,3 Zhao Zhang,4 Hongliang Wu,5 Armin Ghavamian,1 Dongbin Bi,3 Pei Gao,1 Zhao Liu,5 Sentai Ding1,3 1Department of Urology, Shandong Provincial Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250021, People’s Republic of China; 2Department of Urology, Shandong Provincial Hospital West Branch, Jinan, Shandong, 250000, People’s Republic of China; 3Department of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, People’s Republic of China; 4Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266000, People’s Republic of China; 5Department of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People’s Republic of ChinaCorrespondence: Zhao LiuDepartment of Urology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, People’s Republic of ChinaEmail zhao.liu@sdu.edu.cnSentai DingDepartment of Urology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, People’s Republic of ChinaEmail dingsentai@126.comObjective: Prostate cancer (PCa) ranks as the second common malignancy in males worldwide. Although conspicuous progressions in diagnosis and treatment have been achieved in the past decades, the prognosis expectation of PCa remains unsatisfied yet. To improve the prognosis prediction of PCa, more specific biomarkers are needed. In this retrospective research, we focused on βKlotho and ETS-like transcription factor 4 (ELK4), aiming to identify potential prognostic biomarkers for PCa.Methods: Western blotting was used to determine the expression of βKlotho, ELK4, and PARP in C4-2B and PC3 PCa cell lines. CCK-8 assay and colony formation assay were applied to examine the roles of βKlotho and ELK4 in the proliferation of PCa cells. The expression of βKlotho and ELK4 in PCa tissue samples was determined by immunochemistry. Pearson’s χ 2 test and Fisher’s exact test were performed to investigate the associations among βKlotho, ELK4 and various clinical factors. Kaplan–Meier curves and Cox regression model were established to reveal the correlation among βKlotho, ELK4 expression and the prognosis of patients.Results: βKlotho overexpression down-regulated the ELK4 expression, induced apoptosis and inhibited cell proliferation in both C4-2B and PC3 cells, which were reversed by ELK4 overexpression. βKlotho expression in PCa tissue samples had negative correlation with the ELK4 expression, and higher βKlotho expression was associated with lower Gleason score, absent distant metastasis and lower prostate-specific antigen (PSA) level. On the contrast, higher ELK4 expression was correlated with distant metastasis and higher PSA level. Moreover, βKlotho and ELK4 were both recognized as independent factors for the prognosis of patients with PCa.Conclusion: βKlotho inhibits proliferation of prostate cancer cells by downregulating ELK4. Both βKlotho and ELK4 expressions correlate with the prognosis of PCa, which may serve as potential biomarkers for follow-up surveillance and prognostic assessments.Keywords: FGFR, biomarker, prostate cancer, survival

[Objective] Designing a apace grid-based hand-eye calibration method to reduce robot positioning errors and calibration object Recognition errors in automated food production lines. [Methods] Based on the composition of the attitude matrix, the attitude and position of the end of the robot relative to the camera were obtained by translating and rotating the end of the robot, and a registration method using known corresponding point pairs was proposed to reduce the influence of the robot positioning error on the attitude solution in the translation method. Based on the space circle fitting method, a two-point vector method was proposed to solve the end position, which reduced the amount of data collection. A method of fitting the center of the sphere was proposed to compensate for the result of hand-eye calibration. [Results] The average measured error after calibration is 0.8 mm. [Conclusion] The hand-eye system based on the Eye-In-Hand type, can realize high-precision object positioning.

A new genus of malachiine Melyridae, Platyintybia gen. nov., is described based on several male-specific characters, along with description of its type species, Platyintybia zhongshanensis sp. nov., from China. A new combination, Platyintybia sarawakensis (Champion, 1921), comb. nov., is proposed after examining the type specimen; this species is newly recorded from China. A key to the genera of Chinese Apalochrini is provided for the first time.

The objective of this study was to compare the indications and outcomes of the total inferior border ostectomy and T-shape genioplasty. A retrospective study was conducted using the clinical notes and records of patients who underwent total inferior border ostectomy (group 1, n = 42) and T-shape genioplasty (group 2, n = 60). The outcomes were evaluated by assessment of computed tomography images combined with medical records and photographs. Lower facial height, chin width, chin symmetry, and facial proportions, as well as patient satisfaction and complications were investigated. The data were collected preoperatively and 6–24 months postoperatively. All 102 patients showed an improved lower facial contour. No severe complications were observed during the follow-up period. Although the postoperative lower to midfacial height ratios were similar in the two groups (P = 0.080), both the preoperative and postoperative chin width to lower facial height ratios were lower in group 1 (both P < 0.001). A larger amount of chin narrowing, as well as better chin symmetry were observed in group 1 (P < 0.001). In conclusion, compared to the T-shape genioplasty, the total inferior border ostectomy is well suited for a longer, wider, and more asymmetrical chin. The surgical options should be considered and chosen quantitatively to achieve aesthetically pleasing results. [ABSTRACT FROM AUTHOR]

Objective To reduce the contamination rate of urine culture by using nursing project management. Methods A nursing project management team was established, and urine culture test of 318 hospitalized patients were retrospectively analyzed. The current situation of contamination of urine culture was analyzed, and improvement goals and measures were carried out. The contamination rate of urine culture in 272hospitalized patients was observed. Results The contamination rate of urine culture was decreased form 26. 10%(83/318)before implementation of nursing project management to 4. 78%(13/272)after implementation of nursing project management, with a significant difference(P＜0. 05). Conclusion The nursing project management is effective to reduce the contamination rate of urine culture for hospitalized patients, and improve the quality of nephrology nursing (目的 开展护理专案活动降低肾内科住院患者中段尿培养采集污染率。方法 成立护理专案小组, 分析2021年1月—12月收治的318 名住院患者的尿培养采集结果, 确定 “降低肾内科住院患者中段尿培养采集污染率”的活动主题, 分析现状, 找出问题, 制定目标, 制定并实施对策。统计2022年1月—12月开展专案改善活动后的272例患者尿培养采集结果。结果 肾内科住院患者中段尿培养采集污染率由专案改善活动前的26. 10%(83/318)降低至4. 78%(13/272), 差异有统计学意义(P

ABSTRACT Colletotrichum spp. are economically important phytopathogenic fungi that cause anthracnose in a variety of plant species worldwide. Hypovirulence-associated mycoviruses provide new options for the biological control of plant fungal diseases. Here, we found a novel partitivirus from Colletotrichum alienum and named it Colletotrichum alienum partitivirus 1 (CaPV1). CaPV1 contained two dsRNA segments encoding an RNA-dependent RNA polymerase and a capsid protein and was classified under the genus Gammapartitivirus of the family Partitiviridae. CaPV1 significantly decreased host virulence, mycelial growth, appressorial development, and appressorium turgor but increased conidial production with abnormal morphology. In addition, CaPV1 could be successfully transfected into other Colletotrichum species, including C. fructicola, C. spaethianum, and C. gloeosporioides, and caused hypovirulence, indicating the broad application potential of this virus. CaPV1 caused significant transcriptional rewiring of the host fungus C. alienum. Notably, some genes related to vesicle transport in the CaPV1-infected strain were downregulated, consistent with the impaired endocytosis pathway in this fungus. When the Rab gene CaRab7, which is associated with endocytosis in vesicle transport, was knocked out, the virulence of the mutants was reduced. Overall, our findings demonstrated that CaPV1 has the potential to control anthracnose caused by Colletotrichum, and the mechanism by which Colletotrichum induces hypovirulence is caused by affecting vesicle transport.IMPORTANCEColletotrichum is a kind of economically important phytopathogenic fungi that cause anthracnose disease in a variety of plant species worldwide. We found a novel mycovirus of the Gammapartitivirus genus and Partitiviridae family from the phytopathogenic fungus Colletotrichum alienum and named it CaPV1. This study revealed that CaPV1 infection significantly decreased host virulence and fitness by affecting mycelial growth, appressorial development, and appressorium turgor. In addition, CaPV1 could also infect other Colletotrichum species, including C. fructicola, C. spaethianum, and C. gloeosporioides, by viral particle transfection and resulting in hypovirulence of these Colletotrichum species. Transcriptomic analysis showed that CaPV1 caused significant transcriptional rewiring of the host fungus C. alienum, especially the genes involved in vesicle transport. Moreover, endocytosis and gene knockout assays demonstrated that the mechanism underlying CaPV1-induced hypovirulence is, at least in part, caused by affecting the vesicle transport of the host fungus. This study provided insights into the mechanisms underlying the pathogenesis of Colletotrichum species and mycovirus-fungus interactions, linking the role of mycovirus and fungus vesicle transport systems in shaping fungal pathogenicity.

IntroductionThe aim of this study was to assess the correlation between surrogate insulin resistance (IR) indexes and carotid atherosclerosis (CA) in normal-weight populations, as well as compared their ability to predict CA.MethodA total of 26,795 middle-aged and older adult individuals with normal body weights were included. Triglyceride-glucose index (TyG), TyG-body mass index, TyG-waist circumference (TyG-WC), TyG-waist-to-height ratio (TyG-WHtR), visceral adiposity index, Chinese VAI (CVAI) and lipid accumulation product (LAP) were determined using established formulas. The associations between these surrogate indexes and CA were assessed using logistic regression models and restricted cubic spline (RCS) analysis. Receiver operating characteristic curves were utilized to compare the performance of these indexes for predicting CA.ResultThe levels of all seven surrogate indexes of IR were significantly higher in normal-weight individuals with CA than in those without CA (p 89.83, LAP >28.91, TyG-WHtR >4.42 and TyG-WC >704.93. The area under the curve for CVAI was significantly greater than for other indexes (p

Nous analysons les implications, pour l’espagnol en Côte d’Ivoire, de l’intégration du portugais dans l’éducation secondaire. Depuis la création de l’enseignement secondaire sur le modèle de l’ex puissance coloniale vers la fin des années 1940, l’anglais, l’espagnol et l’allemand sont les trois langues étrangères au programme des lycées et collèges ivoiriens. La Côte d’Ivoire et le Portugal ayant signé en 2015 un protocole d’accord en vue de l’intégration du portugais, il est probable que le statu quo ne soit plus de mise dans les années à venir. En plus d’être de la même famille néolatine que l’espagnol, le portugais bénéficie d’une plus grande présence sur le continent africain en tant que langue officielle de cinq pays. Son intégration aura l’avantage de créer une concurrence bénéfique aux acteurs et d’offrir de nouvelles opportunités dans un monde de plus en plus globalisé.

IntroductionPatients with Metabolic Syndrome (MetS) are considered at high-risk for incident stroke. An indicator of visceral adiposity dysfunction, the Chinese Visceral Adiposity Index (CVAI) is used to evaluate the dysfunction of visceral fat. Given the impact of visceral adiposity dysfunction on elevating cardiovascular hazards, this study aimed to examine the association between CVAI and stroke risk in MetS patients.MethodBetween November 2017 and December 2018, a total of 18,974 individuals aged ≥40 underwent standardized in-person clinical interviews in Hunan Province, with 6,732 meeting the criteria for MetS. After the baseline survey was completed, subsequent surveys were conducted biennially. The study was split into two stages performed at baseline and after two years. During the former, receiver-operating characteristic curves were used to assess the accuracy of using baseline CVAI in diagnosing MetS. After two years, we examined the association between CVAI and incident stroke in MetS patients using logistic regression, subgroup analysis, and restricted cubic spline (RCS) analysis.ResultAs evidenced by a higher AUC (AUC:0.741), CVAI demonstrated superior diagnostic performance relative to body mass index (AUC:0.631) and waist circumference (AUC:0.627) in diagnosing MetS. After a 2-year follow-up, 72 MetS patients had a stroke event. There was a robust positive correlation between incident stroke and CVAI in patients with MetS. Each 1 SD increase in CVAI was associated with a 1.52-fold higher risk of stroke after adjustment for confounding factors (aOR=1.52, 95%CI: 1.18-1.95). The RCS demonstrated a reduced risk of stroke for MetS patients when the CVAI was below 110.91. However, no significant correlation was detected between CVAI and stroke in non-MetS patients.ConclusionOur findings recommend CVAI as a superior screening tool for detecting MetS and suggest that reducing CVAI can mitigate the risk of stroke in patients with MetS.

Durante muchos años, la situación del asociacionismo de ELE en Costa de Marfil no ha estado a la altura del dinamismo de su enseñanza no solo en términos demolingüísticos sino también en la producción de materiales didácticos adaptados al alumnado africano. El despertar actual en este ámbito tiene que ver con el poder vinculador de Internet y de las redes sociales por una parte y, por la otra, con la pasión, la imaginación y la visión de las nuevas generaciones de profesorado. Pero este despertar no debe ocultar ni llevar a menospreciar las iniciativas asociativas anteriores, pues aprendiendo de los errores y aciertos del pasado es como las nuevas asociaciones marfileñas de ELE se enfrentarán con éxito a los retos del presente y del futuro.