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Commentators have recommended that forensic scientists' reports contain various disclosures to facilitate comprehension. However, little research has explored whether following best practice recommendations for disclosure impacts on receivers' impressions of the evidence. We examined whether forensic science reports that are more compliant with these best practice recommendations reduced overvaluing of the evidence and sensitized legal and community decision-makers to evidence quality. Across three experiments, 240 legal practitioners/trainees and 566 community decision-makers were presented with a fingerprint or footwear report that was either compliant or non-compliant with best practice recommendations. Participants were then asked to make evaluations and decisions based on the report. We found mixed effects of report compliance. Report compliance affected community participant's evaluations of the persuasiveness of the evidence but had limited impact on the judgments of legal practitioners/trainees. When presented with compliant reports, we found that community participants regarded unknown reliability evidence as less reliable and less persuasive than high reliability evidence, suggesting disclosures helped reduce overvaluing of the evidence and create sensitivity to differences in evidence quality. These results suggest compliance with reporting recommendations does affect community impressions, while only minimally influencing legal impressions of forensic science evidence. The costs and/or benefits of this outcome require further examination., Competing Interests: Declaration of Competing Interest Summersby, S and Ballantyne, K.N. are employees of Victoria Police Forensic Services Department. Edmond G, Kemp, R .I, and Martire, K.A declare no conflicts of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)

There have been numerous calls for increased transparency and disclosure in forensic science. However, there is a paucity of guidance on how to achieve this transparency in reports, and the impacts it may have on criminal justice proceedings. We describe one multi-disciplinary forensic laboratory's journey to fully transparent reporting, disclosing matters of scientific relevance and importance. All expert reports across 17 disciplines now contain information regarding the fundamental principles and methodology, validity and error, assumptions and limitations, competency testing and quality assurance, cognitive factors, and areas of scientific controversy. Staff support for transparent reporting increased following introduction, with most reporting largely positive impacts. A slight increase in questioning in court has been experienced, with increased legal attention paid to the indicia of scientific validity. Transparency in expert forensic science reports is possible, and can improve the use of scientific evidence in courts without compromising the timeliness of service., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests. All authors are employees of Victoria Police Forensic Services Department. The authors were offered a waiver on the article publishing charge for open access publication of this article., (Crown Copyright © 2024 Published by Elsevier B.V.)

This paper distils seven key lessons about 'error' from a collaborative webinar series between practitioners at Victoria Police Forensic Services Department and academics. It aims to provide the common understanding of error necessary to foster interdisciplinary dialogue, collaboration and research. The lessons underscore the inevitability, complexity and subjectivity of error, as well as opportunities for learning and growth. Ultimately, we argue that error can be a potent tool for continuous improvement and accountability, enhancing the reliability of forensic sciences and public trust. It is hoped the shared understanding provided by this paper will support future initiatives and funding for collaborative developments in this vital domain., Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Kristy A. Martire reports financial support was provided by Victoria Police Forensic Services Department. Richard I. Kemp, Rachel A. Searston, Alice Towler, Jason M. Tangen, Gary Edmond, Matthew B. Thompson and Kristy Martire report financial support was provided by 10.13039/501100000923Australian Research Council. Tess M. S. Neal reports financial support was provided by PLuS Alliance Fellowship and Australian-American Fulbright Commission. Carolyn Davis, Stacey Gorski, Zara Lee, Christopher M. Verdon, Gabrielle Jansen, Tanya Lang, Joshua Slocum, Stephanie Summersby, Darren Watson, Melissa V. Werrett and Kaye N. Ballantyne report a relationship with Victoria Police Forensic Services Department that includes: employment. Jason M. Chin is an Editor at Forensic Science International: Synergy. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Authors.)

There is a body of published research that has evaluated the contribution of forensic science to the criminal justice system, but many disciplines of forensic science remain unexplored in this regard. The aim of this study was to examine the contribution that forensic fire examination services provide to criminal investigations and court processes in arson cases. Forensic fire examination services differ in a number of ways to the disciplines covered in previous research on the impact of forensic evidence on justice outcomes. Forensic fire examinations involve a combination of scene examination and laboratory analyses, and the results can provide critical evidence of whether an incident that has occurred is a criminal offence (i.e. whether a fire has occurred as the result of an act of arson). Forensic fire examination is also a discipline that has faced challenges and undergone development in recent decades regarding its scientific basis and the issue of contextual bias. In this study, data were collated for 273 structural fires that were examined by the forensic fire services in Victoria, Australia. In this jurisdiction, scene and laboratory forensic services are delivered within short time frames with a focus on providing impartial scientific and investigative services to assist criminal investigations conducted by police. The current dataset was highly skewed in terms of criminal justice outcomes and was not suitable for conducting the planned statistical analyses. Nonetheless, the pattern of findings obtained suggested that the inclusion of forensic evidence which supported the prosecution of arson may be associated with an increased likelihood of suspects being charged and defendants found guilty. Examination of the decision-making process of the forensic fire examiners has provided insight into the variety of evidence that is considered by forensic experts in reaching the important conclusion about the origin and cause of structural fires., (Crown Copyright © 2021. Published by Elsevier B.V. All rights reserved.)

Forensic scientists endeavour to explain complex scientific principles to legal decision-makers with limited scientific training (e.g., police, lawyers, judges, and jurors). Much of the time this communication is limited to written opinions in expert reports. Notwithstanding considerable scientific research and debate about the best way to communicate forensic science opinions, it is unclear how much of the advice has translated into forensic science practice. In conducting this descriptive study, we examined the reporting practices adopted by forensic scientists across a range of forensic science disciplines. Specifically, we used a quantitative content analysis approach to identify the conclusion types and additional information submitted by forensic scientists in proficiency tests during 2016 ("What would be the wording of the Conclusions in your report?"). Our analysis of 500 randomly selected responses in eight disciplines indicated that the conclusion type which has received the most criticism in recent years (categorical statements) remains the preferred means of expression in a clear majority of responses. We also found that the provision of additional information often considered necessary for rational evaluation of the evidence (e.g., information about reliability and validity) was rarely reported. These results suggest limited engagement with recent recommendations and are concerning given the gravity of the legal decisions that hinge on accurate and transparent forensic science communication., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 The Chartered Society of Forensic Sciences. All rights reserved.)

There is limited information available about the impact of chemical trace evidence and it has tended to be anecdotal and mostly pertaining to court outcomes. Very little is known about the use of chemical trace evidence by police investigators or the impact that this evidence form has on criminal investigations. This survey, which was conducted in Victoria, Australia, was aimed at addressing these inadequacies by capturing information from police investigators about: (i) the purpose of using chemical trace and other forensic services; (ii) the expectation of what value forensic services would provide; (iii) the actual impact of forensic evidence in specified cases; and (iv) the general perceptions of forensic science. Police officers who were the lead investigators in a sample of criminal investigations were selected as the subjects for this survey. Each of the sample cases included chemical trace evidence and many of the cases also included other forms of forensic evidence. The police investigators indicated that they use chemical trace evidence with the expectation that it will assist decision-making in their investigations and contribute to building a case for court. Survey responses indicated that chemical trace evidence can impact on multiple stages of a case and that this form of evidence can play a part in guiding police investigators in making decisions about how their cases progress through the criminal justice system. It was found that an important aspect of the impact of chemical trace evidence can involve connections with other forensic and non-forensic evidence in the cases. The provision of preliminary results, prior to the formal written reports that are issued for use in court, enables chemical trace evidence to contribute timely support to investigations. The findings of this survey study contradict prevailing perceptions that the contribution of chemical trace evidence is limited to the presentation of evidence in court., Competing Interests: Declaration of Competing Interest Peter Woodman and Kaye Ballantyne were employees of VPFSD at the time of the study. However, the study was conducted as an independent piece of research under the supervision of the other authors who are external to the organisation., (Crown Copyright © 2020. Published by Elsevier B.V. All rights reserved.)

The focus of this research was to examine the contribution chemical trace evidence makes to criminal justice outcomes. The aim of this work was to place the discipline of chemical trace evidence under the spotlight as there is a dearth of robust research on the impact of this discipline. In this study, data relating to the forensic examinations in a sample of 238 cases which included chemical trace evidence, was collated with data from police investigations and court processes. The findings show that chemical trace evidence is frequently used in combination with other forensic disciplines to support the progress of high-level criminal cases through the justice system. Due to characteristics of how the criminal cases in the dataset were investigated and prosecuted, in combination with the methodology applied in this study, the impact of forensic evidence on the decision to charge suspects could not be analysed quantitatively. However, the impact of forensic evidence on court outcomes in the sample of cases was analysed using methodology that considered the results of the examinations, and the ability of the evidence to provide support for the inclusion or exclusion of persons of interest. The possibility of chemical trace evidence having impact when applied in combination with other forensic disciplines was also examined. It was found that biological examination results was a significant standalone predictor of court outcomes. In contrast, chemical trace examinations did not predict court outcomes when considered as a standalone predictor but examination results of chemical trace evidence in combination with ballistics/tool marks was significantly associated with court outcomes. The findings of this research indicate that, to assess the full impact of any discipline of forensic evidence on the criminal justice system, the analysis must take into account the potential for important synergies that may exist with other forensic and non-forensic evidence., Competing Interests: Declaration of Competing Interest Peter Woodman and Kaye Ballantyne were employees of VPFSD at the time of the study. However, the study was conducted as an independent piece of research under the supervision of the other authors who are external to the organisation., (Copyright © 2019. Published by Elsevier B.V.)

We do not know how often false positive reports are made in a range of forensic science disciplines. In the absence of this information it is important to understand the naive beliefs held by potential jurors about forensic science evidence reliability. It is these beliefs that will shape evaluations at trial. This descriptive study adds to our knowledge about naive beliefs by: (1) measuring jury-eligible (lay) perceptions of reliability for the largest range of forensic science disciplines to date, over three waves of data collection between 2011 and 2016 (n=674); (2) calibrating reliability ratings with false positive report estimates; and (3) comparing lay reliability estimates with those of an opportunity sample of forensic practitioners (n=53). Overall the data suggest that both jury-eligible participants and practitioners consider forensic evidence highly reliable. When compared to best or plausible estimates of reliability and error in the forensic sciences these views appear to overestimate reliability and underestimate the frequency of false positive errors. This result highlights the importance of collecting and disseminating empirically derived estimates of false positive error rates to ensure that practitioners and potential jurors have a realistic impression of the value of forensic science evidence., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Bloodstain evidence can be very powerful evidence in assault related crimes. Determination of the distance that blood droplets may travel as a result of an impact into liquid blood may be of significance to corroborate or disprove a version of events, provide likely scenarios, or help determine the culpability of a person in determining their proximity to the blood shedding event. It was the aim of this research to determine the potential maximum distance blood droplets travel horizontally following a vertical impact into liquid blood. A custom apparatus was designed and constructed to replicate a vertical impact of a timber weapon, rotating on a fixed axis at one end, striking a pool of liquid blood. The device was positioned at three different levels of elevation to replicate an impact to the head of a person near ground level, a seated or kneeling height and standing height. Overall, the results indicated that the application of kinetic energy of between 1 and 5J at a height of 1780mm led to the blood droplets travelling a maximum horizontal distance of 5361mm (and average maximum distance of 4981mm). The horizontal distance blood droplets may travel upon impact does not appear to follow a linear trend with differing kinetic energy, but is affected by the applied force and release height in a curvilinear relationship. The results provide a valuable tool to bloodstain pattern analysts and investigators in determining search zones within a scene, as well as providing information about the proximity of an individual to an impact event., (Crown Copyright © 2018. Published by Elsevier B.V. All rights reserved.)

Questions relating to how DNA from an individual got to where it was recovered from and the activities associated with its pickup, retention and deposition are increasingly relevant to criminal investigations and judicial considerations. To address activity level propositions, investigators are typically required to assess the likelihood that DNA was transferred indirectly and not deposited through direct contact with an item or surface. By constructing a series of Bayesian networks, we demonstrate their use in assessing activity level propositions derived from a recent legal case involving the alleged secondary transfer of DNA to a surface following a handshaking event. In the absence of data required to perform the assessment, a set of handshaking simulations were performed to obtain probabilities on the persistence of non-self DNA on the hands following a 40min, 5h or 8h delay between the handshake and contact with the final surface (an axe handle). Variables such as time elapsed, and the activities performed and objects contacted between the handshake and contact with the axe handle, were also considered when assessing the DNA results. DNA from a known contributor was transferred to the right hand of an opposing hand-shaker (as a depositor), and could be subsequently transferred to, and detected on, a surface contacted by the depositor 40min to 5h post-handshake. No non-self DNA from the known contributor was detected in deposits made 8h post-handshake. DNA from the depositor was generally detected as the major or only contributor in the profiles generated. Contributions from the known contributor were minor, decreasing in presence and in the strength of support for inclusion as the time between the handshake and transfer event increased. The construction of a series of Bayesian networks based on the case circumstances provided empirical estimations of the likelihood of direct or indirect deposition. The analyses and conclusions presented demonstrate both the complexity of activity level assessments concerning DNA evidence, and the power of Bayesian networks to visualise and explore the issues of interest for a given case., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Genetic profiling of DNA collected from fingerprints that have been exposed to various enhancement techniques is routine in many forensic laboratories. As a result of direct contact with fingermark residues during treatment, there is concern around the DNA contamination risk of dusting fingermarks with fingerprint brushes. Previous studies have demonstrated the potential for cross-contamination between evidentiary items through various mechanisms, highlighting the risk of using the same fingerprint brush to powder multiple surfaces within and between crime-scenes. Experiments were performed to assess the contamination risk of reused fingerprint brushes through the transfer of dried saliva and skin deposits from and to glass surfaces with new unused squirrel hair and fiberglass brushes. Additional new unused brushes and brushes previously used in casework were also tested for their ability to contaminate samples. In addition, the ability to eradicate DNA from used squirrel hair and fiberglass fingerprint brushes was assessed using a 1% sodium hypochlorite solution and a 5% solution of a commercially available alternative, Virkon. DNA profiling results from surfaces contacted by treated and untreated brushes were compared to determine the effectiveness of the devised cleaning protocol. Brush durability was also assessed over multiple wash/rinse/dry cycles with both agents. Varying amounts of DNA-containing material were collected and transferred by squirrel hair and fiberglass brushes, with detectability on the secondary surface dependent on the biological nature of the material being transferred. The impact of DNA contamination from dirty fingerprint brushes was most apparent in simulations involving the transfer of dried saliva and brushes previously used in casework, while minimal transfer of touch DNA was observed. Alarmingly, large quantities of DNA were found to reside on new unused squirrel hair brushes, while no DNA was detected on new unused fiberglass brushes or brushes sold as DNA-free. Squirrel hair brushes were easily and effectively cleaned with both hypochlorite and Virkon, with no evidence of DNA transfer between exhibits by treated brushes. Brushes were still deemed useable after multiple cleaning cycles with either agent. In contrast, fiberglass bristles became tangled and matted when wet and could not be cleaned effectively using either method. It is recommended they are disposed of following use. Each laboratory should consider their current circumstances before adapting a cleaning method. The implementation of a program to monitor the effectiveness of the cleaning regime is also advised., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Peer review features prominently in the forensic sciences. Drawing on recent research and studies, this article examines different types of peer review, specifically: editorial peer review; peer review by the scientific community; technical and administrative review; and verification (and replication). The article reviews the different meanings of these quite disparate activities and their utility in relation to enhancing performance and reducing error. It explains how forensic practitioners should approach and use peer review, as well as how it should be described in expert reports and oral testimony. While peer review has considerable potential, and is a key component of modern quality management systems, its actual value in most forensic science settings has yet to be determined. In consequence, forensic practitioners should reflect on why they use specific review procedures and endeavour to make their actual practices and their potential value transparent to consumers; whether investigators, lawyers, jurors or judges. Claims that review increases the validity of a scientific technique or accuracy of opinions within a particular case should be avoided until empirical evidence is available to support such assertions., (Copyright © 2017 Elsevier B.V. All rights reserved.)

During the evaluation of forensic DNA evidence in court proceedings, the emphasis previously placed on the source of the DNA is progressively shifting to the consideration of the activities resulting in its deposition. While direct contact and deposition may be a likely explanation, alternative scenarios involving DNA transfer through a secondary person or medium are important to consider. Here we assessed whether non-self DNA, indirectly transferred via a handshake, could be detected on surfaces contacted by the opposing hand-shaker after 15min, and considered the variables affecting its persistence in subsequent contacts. In general, the depositor of the handprint was the major contributor to DNA profiles collected from handprints placed on glass plates. Minor contributions from the opposing hand-shaker (as a known contributor) were detected at a lower rate, decreasing as the number of contacted items increased post-handshake. Delays in deposition also affected the detection of the opposing hand-shaker, with a 15min delay between handshaking and contact resulting in the reduced presence, and corresponding LRs, of the known contributor. The handprint depositor was excluded from their own handprint on several occasions, including instances where the opposing hand-shaker was not excluded from the same profile. Several factors appeared to strongly influence the detection of both the depositor and contributing individual involved in the handshake. The relative shedding ability of the pair had the largest effect, where good shedders (whether depositor or contributor) could swamp poor to moderate shedders, while the pairing of two moderate or two poor shedders could result in the detection of both individuals. When the deposition of a handprint was delayed, the activities performed by the individual had a substantial effect on the resultant detection of the contributing profile - multiple contacts with the same items increased the likelihood that the known contributor's DNA would be retained and subsequently detected, through the parking and re-transfer of DNA on used items., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Aboriginal Australians are one of the more poorly studied populations from the standpoint of human evolution and genetic diversity. Thus, to investigate their genetic diversity, the possible date of their ancestors' arrival and their relationships with neighboring populations, we analyzed mitochondrial DNA (mtDNA) diversity in a large sample of Aboriginal Australians. Selected mtDNA single-nucleotide polymorphisms and the hypervariable segment haplotypes were analyzed in 594 Aboriginal Australians drawn from locations across the continent, chiefly from regions not previously sampled. Most (~78%) samples could be assigned to mtDNA haplogroups indigenous to Australia. The indigenous haplogroups were all ancient (with estimated ages >40 000 years) and geographically widespread across the continent. The most common haplogroup was P (44%) followed by S (23%) and M42a (9%). There was some geographic structure at the haplotype level. The estimated ages of the indigenous haplogroups range from 39 000 to 55 000 years, dates that fit well with the estimated date of colonization of Australia based on archeological evidence (~47 000 years ago). The distribution of mtDNA haplogroups in Australia and New Guinea supports the hypothesis that the ancestors of Aboriginal Australians entered Sahul through at least two entry points. The mtDNA data give no support to the hypothesis of secondary gene flow into Australia during the Holocene, but instead suggest long-term isolation of the continent.

Objective: Understanding the origins of Aboriginal Australians is crucial in reconstructing the evolution and spread of Homo sapiens as evidence suggests they represent the descendants of the earliest group to leave Africa. This study analyzed a large sample of Y-chromosomes to answer questions relating to the migration routes of their ancestors, the age of Y-haplogroups, date of colonization, as well as the extent of male-specific variation., Methods: Knowledge of Y-chromosome variation among Aboriginal Australians is extremely limited. This study examined Y-SNP and Y-STR variation among 657 self-declared Aboriginal males from locations across the continent. 17 Y-STR loci and 47 Y-SNPs spanning the Y-chromosome phylogeny were typed in total., Results: The proportion of non-indigenous Y-chromosomes of assumed Eurasian origin was high, at 56%. Y lineages of indigenous Sahul origin belonged to haplogroups C-M130*(xM8,M38,M217,M347) (1%), C-M347 (19%), K-M526*(xM147,P308,P79,P261,P256,M231,M175,M45,P202) (12%), S-P308 (12%), and M-M186 (0.9%). Haplogroups C-M347, K-M526*, and S-P308 are Aboriginal Australian-specific. Dating of C-M347, K-M526*, and S-P308 indicates that all are at least 40,000 years old, confirming their long-term presence in Australia. Haplogroup C-M347 comprised at least three sub-haplogroups: C-DYS390.1del, C-M210, and the unresolved paragroup C-M347*(xDYS390.1del,M210)., Conclusions: There was some geographic structure to the Y-haplogroup variation, but most haplogroups were present throughout Australia. The age of the Australian-specific Y-haplogroups suggests New Guineans and Aboriginal Australians have been isolated for over 30,000 years, supporting findings based on mitochondrial DNA data. Our data support the hypothesis of more than one route (via New Guinea) for males entering Sahul some 50,000 years ago and give no support for colonization events during the Holocene, from either India or elsewhere., (© 2015 Wiley Periodicals, Inc.)

Effective sampling of biological material is critical to the ability to acquire DNA profiles of probative value. The main methods of collection are swabbing, tapelifting, or direct excision. This chapter describes the key aspects to consider when applying these methods, in addition to suggested procedures for swabbing and tapelifting. Important issues to be considered, such as exhibit triaging, pre-examination preparation, contamination risk reduction, sample localization, sample identification, and sample prioritization as well as aspects of record keeping, packaging, and storage, are also raised.

The introduction of profiling systems with increased sensitivity has led to a concurrent increase in the risk of detecting contaminating DNA in forensic casework. To evaluate the contamination risk of tools used during exhibit examination we have assessed the occurrence and level of DNA transferred between mock casework exhibits, comprised of cotton or glass substrates, and high-risk vectors (scissors, forceps, and gloves). The subsequent impact of such transfer in the profiling of a target sample was also investigated. Dried blood or touch DNA, deposited on the primary substrate, was transferred via the vector to the secondary substrate, which was either DNA-free or contained a target sample (dried blood or touch DNA). Pairwise combinations of both heavy and light contact were applied by each vector in order to simulate various levels of contamination. The transfer of dried blood to DNA-free cotton was observed for all vectors and transfer scenarios, with transfer substantially lower when glass was the substrate. Overall touch DNA transferred less efficiently, with significantly lower transfer rates than blood when transferred to DNA-free cotton; the greatest transfer of touch DNA occurred between cotton and glass substrates. In the presence of a target sample, the detectability of transferred DNA decreased due to the presence of background DNA. Transfer had no impact on the detectability of the target profile, however, in casework scenarios where the suspect profiles are not known, profile interpretation becomes complicated by the addition of contaminating alleles and the probative value of the evidence may be affected. The results of this study reiterate the need for examiners to adhere to stringent laboratory cleaning protocols, particularly in the interest of contamination minimisation, and to reduce the handling of items to prevent intra-item transfer., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)

Recently introduced rapidly mutating Y-chromosomal short tandem repeat (RM Y-STR) loci, displaying a multiple-fold higher mutation rate relative to any other Y-STRs, including those conventionally used in forensic casework, have been demonstrated to improve the resolution of male lineage differentiation and to allow male relative separation usually impossible with standard Y-STRs. However, large and geographically-detailed frequency haplotype databases are required to estimate the statistical weight of RM Y-STR haplotype matches if observed in forensic casework. With this in mind, the Italian Working Group (GEFI) of the International Society for Forensic Genetics launched a collaborative exercise aimed at generating an Italian quality controlled forensic RM Y-STR haplotype database. Overall 1509 male individuals from 13 regional populations covering northern, central and southern areas of the Italian peninsula plus Sicily were collected, including both "rural" and "urban" samples classified according to population density in the sampling area. A subset of individuals was additionally genotyped for Y-STR loci included in the Yfiler and PowerPlex Y23 (PPY23) systems (75% and 62%, respectively), allowing the comparison of RM and conventional Y-STRs. Considering the whole set of 13 RM Y-STRs, 1501 unique haplotypes were observed among the 1509 sampled Italian men with a haplotype diversity of 0.999996, largely superior to Yfiler and PPY23 with 0.999914 and 0.999950, respectively. AMOVA indicated that 99.996% of the haplotype variation was within populations, confirming that genetic-geographic structure is almost undetected by RM Y-STRs. Haplotype sharing among regional Italian populations was not observed at all with the complete set of 13 RM Y-STRs. Haplotype sharing within Italian populations was very rare (0.27% non-unique haplotypes), and lower in urban (0.22%) than rural (0.29%) areas. Additionally, 422 father-son pairs were investigated, and 20.1% of them could be discriminated by the whole set of 13 RM Y-STRs, which was very close to the theoretically expected estimate of 19.5% given the mutation rates of the markers used. Results obtained from a high-coverage Italian haplotype dataset confirm on the regional scale the exceptional ability of RM Y-STRs to resolve male lineages previously observed globally, and attest the unsurpassed value of RM Y-STRs for male-relative differentiation purposes., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)