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I here review the potential effects of climate change on aphidophagous ladybirds and their future diversity. Aphidophagous ladybirds face challenges arising directly from climatic change and indirect challenges due to the effect of climate on their aphid prey and other organisms with which they interact. Ladybirds show at least some potential to respond to changes of climate through genetic change or phenotypic plasticity, notably through changes in colour pattern, dormancy and voltinism but also through thermal physiology and microhabitat or habitat plasticity. Phenological changes will likely occur in many aphidophagous species arising from relations with their prey. Changes in aphid abundance may lead to changes in ladybird prey associations over the season. The tendency to prey on multiple aphid species will insulate many ladybird predators from decline resulting from changes in the abundance of particular aphids. Like other insects, the geographic ranges of aphidophagous ladybird species will change as they track the climate. This is evidenced by the fossil record of ladybirds and by recent changes in ladybird biogeography. Such changes may be accompanied by a degree of thermal or phenological adaptation. It is likely that cold-adapted ladybirds and some island species will go extinct. Specialised species may also be threatened. A problem in assessing the threat to the latter is the minimal amount of research carried out on specialised species. In summary, aphidophagous ladybirds exhibit some potential resilience against climate change, but future climatic effects on their overall biodiversity are not fully clear.

I here review the potential effects of climate change on aphidophagous ladybirds and their future diversity. Aphidophagous ladybirds face challenges arising directly from climatic change and indirect challenges due to the effect of climate on their aphid prey and other organisms with which they interact. Ladybirds show at least some potential to respond to changes of climate through genetic change or phenotypic plasticity, notably through changes in colour pattern, dormancy and voltinism but also through thermal physiology and microhabitat or habitat plasticity. Phenological changes will likely occur in many aphidophagous species arising from relations with their prey. Changes in aphid abundance may lead to changes in ladybird prey associations over the season. The tendency to prey on multiple aphid species will insulate many ladybird predators from decline resulting from changes in the abundance of particular aphids. Like other insects, the geographic ranges of aphidophagous ladybird species will change as they track the climate. This is evidenced by the fossil record of ladybirds and by recent changes in ladybird biogeography. Such changes may be accompanied by a degree of thermal or phenological adaptation. It is likely that cold-adapted ladybirds and some island species will go extinct. Specialised species may also be threatened. A problem in assessing the threat to the latter is the minimal amount of research carried out on specialised species. In summary, aphidophagous ladybirds exhibit some potential resilience against climate change, but future climatic effects on their overall biodiversity are not fully clear.

Establishment and spread of invasive species can be facilitated by lack of natural enemies in the invaded area. Host-range evolution of natural enemies augments their ability to reduce the impact of the invader and could enhance their value for biological control. We assessed the potential of the Drosophila parasitoid, Leptopilina heterotoma (Hymenoptera: Figitidae), to exploit the invasive pest Drosophila suzukii by focusing on three performance indices: (i) attack rate; (ii) host killing, consisting of killing rate and lethal attack rate (killing efficiency); and (iii) successful offspring development (reproductive success). We found significant intraspecific variation in attack rate and killing rate and lethal attack rate among seven European populations, but offspring generally failed to successfully develop from the D. suzukii host. We crossed these European lines to create a genetically variable source population and performed a half-sib analysis to quantify genetic variation. Using a Bayesian animal model, we found that attack rate and killing rate had a heritability of h(2)=0.2, lethal attack rate h(2)=0.4, and offspring development h(2)=0. We then artificially selected wasps with the highest killing rate of D. suzukii for seven generations to test whether host-killing could be improved. There was a small and inconsistent response to selection in the three selection lines. Realized heritability (hr(2)) after four generations of selection was 0.17 but near zero after seven generations of selection. The genetic response might have been masked by an increased D. suzukii fitness resulting from adaptation to laboratory conditions. Our study reveals that native, European, L. heterotoma can attack the invasive pest, D. suzukii and significantly reduce fly survival and that different steps of the parasitization process need to be considered in the evolution of host-range. It highlights how evolutionary principles can be applied to optimize performance of native species for biological control.

Hosts can carry many viruses in their bodies, but not all of them cause disease. We studied ants as a social host to determine both their overall viral repertoire and the subset of actively infecting viruses across natural populations of three subfamilies: the Argentine ant (Linepithema humile, Dolichoderinae), the invasive garden ant (Lasius neglectus, Formicinae) and the red ant (Myrmica rubra, Myrmicinae). We used a dual sequencing strategy to reconstruct complete virus genomes by RNA-seq and to simultaneously determine the small interfering RNAs (siRNAs) by small RNA sequencing (sRNA-seq), which constitute the host antiviral RNAi immune response. This approach led to the discovery of 41 novel viruses in ants and revealed a host-ant specific RNAi response (21 vs. 22 nt siRNAs) in the different ant species. The efficiency of the RNAi response (sRNA/RNA read count ratio) depended on the virus and the respective ant species, but not its population. Overall, we found the highest virus abundance and diversity per population inLi. humile, followed byLa. neglectusandM. rubra. Argentine ants also shared a high proportion of viruses between populations, whilst overlap was nearly absent inM. rubra. Only a single of the total 59 viruses in our study caused active infection in more than one ant species, whilst six viruses infected one, but only contaminated another ant species. Disentangling active infection from contamination thus allowed us to show high host-specificity of active viral infections versus a decent degree of spillover of non-infecting viral contaminants across ant species, providing relevant information for ecosystem management.

The root-knot nematode, Meloidogyne incognita, is a major pest in tomato production. Paenibacillus polymyxa, which is primarily found in soil and colonizing roots, is considered a successful biocontrol organism against many pathogens. To evaluate the biocontrol capacity of P. polymyxa LMG27872 against M. incognita in tomato, experiments were conducted both in vitro and in vivo. A dose-response effect [30, 50, and 100% (108 CFU/mL)] of bacterial suspensions (BSs) on growth and tomato susceptibility to M. incognita with soil drenching as a mode of application was first evaluated. The results show that the biological efficacy of P. polymyxa LMG27872 against M. incognita parasitism in tomato was dose-dependent. A significantly reduced number of galls, egg-laying females (ELF), and second-stage juveniles (J2) were observed in BS-treated plants, in a dose-dependent manner. The effect of P. polymyxa on tomato growth was also dose-dependent. A high dose of BSs had a negative effect on growth; however, this negative effect was not observed when the BS-treated plants were challenged with M. incognita, indicating tolerance or a defense priming mechanism. In subsequent in vivo experiments, the direct effect of BSs was evaluated on J2 mortality and egg hatching of M. incognita. The effect of BS on J2 mortality was observed from 12 to 24 h, whereby M. incognita J2 was significantly inhibited by the BS treatment. The effect of P. polymyxa on M. incognita egg hatching was also dependent on the BS dose. The results show a potential of P. polymyxa LMG27872 to protect plants from nematode parasitism and its implementation in integrated nematode management suitable for organic productions.

Floral nectar is ubiquitously colonized by a variety of microorganisms among which yeasts and bacteria are the most common. Microorganisms inhabiting floral nectar can alter several nectar traits, including nectar odor by producing microbial volatile organic compounds (mVOCs). Evidence showing that mVOCs can affect the foraging behavior of insect pollinators is increasing in the literature, whereas the role of mVOCs in altering the foraging behavior of third-trophic level organisms such as insect parasitoids is largely overlooked. Parasitoids are frequent visitors of flowers and are well known to feed on nectar. In this study, we isolated bacteria inhabiting floral nectar of buckwheat, Fagopyrum esculentum (Polygonales: Polygonaceae), to test the hypothesis that nectar bacteria affect the foraging behavior of the egg parasitoid Trissolcus basalis (Hymenoptera: Scelionidae) via changes in odors of nectar. In behavioral assays, we found that T. basalis wasps are attracted toward nectar fermented by 4 out of the 14 bacterial strains isolated, which belong to Staphylococcus epidermidis, Terrabacillus saccharophilus (both Firmicutes), Pantoea sp. (Proteobacteria), and Curtobacterium sp. (Actinobacteria). Results of chemical investigations revealed significant differences in the volatile blend composition of nectars fermented by the bacterial isolates. Our results indicate that nectar-inhabiting bacteria play an important role in the interactions between flowering plants and foraging parasitoids. These results are also relevant from an applied perspective as flowering resources, such as buckwheat, are largely used in agriculture to promote conservation biological control of insect pests.

Aim: The present study aimed to investigate the antifungal effects of fenhexamid, cyprodinil+fludioxanil, Bacillus subtilis QST 713, Trichoderma secondary metabolites (EGE-K-38 and EGE-K-71) on 6 isolates of necrotrophic fungi (Botrytis cinerea) under in- vitro conditions. Methodology: A dual culture technique was used to evaluate the effects of volatile compounds produced by Trichoderma in culture media against Botrytis cinerea isolates. For non-volatile and volatile metabolites, measurements for growth inhibition were made with the mixture of filter sterilized liquid medium of Trichoderma and potato dextrose agar. The fungicidal effects of these non-volatile metabolites and fungicides were first evaluated according to the effective concentration (EC50) data of growing mycelium inhibition of disease agents. Results: According to the results obtained, the drug/agent with the lowest fungicidal activity was determined as fenhexamide, which seems to have the lowest EC50 value of 0.05 ug m l-1. Both non-volatile metabolites and volatile metabolites of Trichoderma strains showed strong inhibition against B. cinerea isolates under experimental conditions. The highest growth inhibition percentage was determined with volatile metabolites of Trihoderma atroviride EGE-K-71 strain as 71.8%. Interpretation: The study suggest that filtrates that contain secondary metabolites and volatile compounds of Trichoderma strains produced in both liquid culture and dual culture conditions can be used as an effective fungal control agents against pre and postharvest contamination of grapes with Botrytis cinerea.

This article charts the popularization of and eventual disengagement with an approach to wound infection control centered on the medical efficacy of living beings (maggots) in 1920s and 1930s America. Baltimore surgeon William Stevenson Baer successfully drew on his wartime experience to promote the use of sterile or “surgical” maggots in the treatment for deep-seated bone infection at this time. This article situates the practices he promoted in the context of President Herbert Hoover's contemporary establishment of the “associative state,” thereby drawing out the relevance of the latter to medical governance. In so doing, it conveys an image of the development of early twentieth-century infection control that contrasts with narratives centered on medically productive aspects of biomedical industrialization and specialization. The incorporation of surgical maggot research and evaluation within the United States Department of Agriculture played a critical role in the subsequent abandonment of the use of maggots as medical aides. Hoover's orientation of this institution towards the support of large-scale private enterprise helped motivate efforts to synthetically replicate (and thereby displace) maggots' medical efficacy. Ultimately, maggot-based therapeutics proved incompatible with conditions created by the orientation of American governmental institutions towards corporate subsidy rather than the support of individuals.

Ladybirds (Coleoptera: Coccinellidae) provide services that are critical to food production, and they fulfill an ecological role as a food source for predators. The richness, abundance, and distribution of ladybirds, however, are compromised by many anthropogenic threats. Meanwhile, a lack of knowledge of the conservation status of most species and the factors driving their population dynamics hinders the development and implementation of conservation strategies for ladybirds. We conducted a review of the literature on the ecology, diversity, and conservation of ladybirds to identify their key ecological threats. Ladybird populations are most affected by climate factors, landscape composition, and biological invasions. We suggest mitigating actions for ladybird conservation and recovery. Short-term actions include citizen science programs and education, protective measures for habitat recovery and threatened species, prevention of the introduction of non-native species, and the maintenance and restoration of natural areas and landscape heterogeneity. Mid-term actions involve the analysis of data from monitoring programs and insect collections to disentangle the effect of different threats to ladybird populations, understand habitat use by taxa on which there is limited knowledge, and quantify temporal trends of abundance, diversity, and biomass along a management-intensity gradient. Long-term actions include the development of a worldwide monitoring program based on standardized sampling to fill data gaps, increase explanatory power, streamline analyses, and facilitate global collaborations.Las catarinas (Coleoptera: Coccinellidae) proporcionan servicios que son críticos para la producción de alimento, y juegan un papel ecológico como fuente de alimento para depredadores. Sin embargo, la riqueza, abundancia y distribución de catarinas están en peligro debido a muchas amenazas antropogénicas. La carencia de conocimiento sobre el estatus de conservación de la mayoría de las especies y los factores que inciden en su dinámica poblacional dificulta el desarrollo e implementación de estrategias de conservación para las catarinas. Realizamos una revisión de la literatura sobre la ecología, diversidad y conservación de catarinas para identificar sus amenazas ecológicas clave. Las poblaciones de catarinas fueron afectadas mayormente por factores climáticos, composición del paisaje e invasiones biológicas. Proponemos acciones de mitigación para la conservación y recuperación de catarinas. Acciones a corto plazo incluyen programas de ciencia y educación ciudadana, medidas de protección para la recuperación de hábitat y de especies amenazadas, prevención de la introducción de especies no nativas y el mantenimiento y restauración de áreas naturales y la heterogeneidad del paisaje. Acciones a mediano plazo implican el análisis de datos obtenidos de programas de monitoreo y colecciones de insectos para desenmarañar el efecto de las diferentes amenazas a las poblaciones de catarinas, comprender el uso del hábitat por taxa de los que se tiene conocimiento limitado y cuantifica las tendencias temporales de la abundancia, diversidad y biomasa a lo largo de un gradiente de intensidad de manejo. Acciones a largo plazo incluyen el desarrollo de un programa de monitoreo a nivel mundial basado en muestreos estandarizados para subsanar la falta de datos, incrementar el poder explicativo, optimizar los análisis y facilitar colaboraciones globales.随着世界范围内人类与野生动物的冲突不断升级, 对野生动物的宽容和接纳等概念变得越来越重要。然而, 当代保护研究表明, 人们对人类与野生动物积极互作的认识有限, 导致可能难以准确描述人类与动物的相遇。如果不解决这些局限性, 就会导致野生动物和景观管理计划的设计和实施存在不足, 以及对本土生态知识的否定。我们利用印度喀拉拉邦瓦亚纳德野生动物保护区森林中的阿迪瓦西族Kattunayakans部落的民族志证据, 来研究印度原住民对于人类与野生动物共存的观点。通过定性的实地研究(包括访谈和在森林样带调查), 我们发现Kattunayakans部落表现出对野生动物的宽容和接纳, 其特点是深入的共存, 包括三个中心思想:野生动物是有理性的交流者;野生动物是神、老师和平等的族群;野生动物是实行 “dharmam”的具有共同起源的亲戚。我们认为, 充分理解以上几点有助于将Kattunayakan部落的观点引入印度的森林管理, 并更广泛地解决人类与野生动物的冲突。.

Bacillus velezensis is considered as model species for plant-associated bacilli providing benefits to its host such as protection against phytopathogens. This is mainly due to the potential to secrete a wide range of secondary metabolites with specific and complementary bioactivities. This metabolite arsenal has been quite well defined genetically and chemically but much remains to be explored regarding how it is expressed under natural conditions and notably how it can be modulated upon interspecies interactions in the competitive rhizosphere niche. Here, we show that B. velezensis can mobilize a substantial part of its metabolome upon the perception of Pseudomonas, as a soil-dwelling competitor. This metabolite response reflects a multimodal defensive strategy as it includes polyketides and the bacteriocin amylocyclicin, with broad antibiotic activity, as well as surfactin lipopeptides, contributing to biofilm formation and enhanced motility. Furthermore, we identified the secondary Pseudomonas siderophore pyochelin as an info-chemical, which triggers this response via a mechanism independent of iron stress. We hypothesize that B. velezensis relies on such chelator sensing to accurately identify competitors, illustrating a new facet of siderophore-mediated interactions beyond the concept of competition for iron and siderophore piracy. This phenomenon may thus represent a new component of the microbial conversations driving the behavior of members of the rhizosphere community.

The risks of Aedes aegypti and Aedes albopictus nuisance and vector-borne diseases are rising and the adverse effects of broad-spectrum insecticide application have promoted species-specific techniques, such as sterile insect technique (SIT) and other genetic strategies, as contenders in their control operations. When specific vector suppression is proposed, potential effects on predators and wider ecosystem are some of the first stakeholder questions. These are not the only Aedes vectors of human diseases, but are those for which SIT and genetic strategies are of most interest. They vary ecologically and in habitat origin, but both have behaviorally human-adapted forms with expanding ranges. The aquatic life stages are where predation is strongest due to greater resource predictability and limited escape opportunity. These vectors' anthropic forms usually use ephemeral water bodies and man-made containers as larval habitats; predators that occur in these are mobile, opportunistic and generalist. No literature indicates that any predator depends on larvae of either species. As adults, foraging theory predicts these mosquitoes are of low profitability to predators. Energy expended hunting and consuming will mostly outweigh their energetic benefit. Moreover, as adult biomass is mobile and largely disaggregated, any predator is likely to be a generalist and opportunist. This work, which summarizes much of the literature currently available on the predators of Ae. aegypti and Ae. albopictus, indicates it is highly unlikely that any predator species depends on them. Species-specific vector control to reduce nuisance and disease is thus likely to be of negligible or limited impact on nontarget predators. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Mycoviruses are viruses that infect fungi, and hypovirulence-associated mycoviruses have the potential to control fungal diseases. However, it is unclear how mycovirus-mediated hypovirulent strains live and survive in the field, and no mycovirus has been applied for field crop protection. In this study, we found that a previously identified small DNA mycovirus (SsHADV-1) can convert its host, Sclerotinia sclerotiorum, from a typical necrotrophic pathogen to a beneficial endophytic fungus. SsHADV-1 downregulates the expression of key pathogenicity factor genes in S. sclerotiorum during infection. When growing in rapeseed, the SsHADV-1-infected strain DT-8 significantly regulates the expression of rapeseed genes involved in defense, hormone signaling, and circadian rhythm pathways. As a result, plant growth is promoted and disease resistance is enhanced. Field experiments showed that spraying DT-8 at the early flowering stage can reduce the disease severity of rapeseed stem rot by 67.6% and improve yield by 14.9%. Moreover, we discovered that SsHADV-1 could also infect other S. sclerotiorum strains on DT-8-inoculated plants and that DT-8 could be recovered from dead plants. These findings suggest that the mycoviruses may have the ability to shape the origin of endophytism. Our discoveries suggest that mycoviruses may influence the origin of endophytism and may also offer a novel strategy for disease control in which mycovirus-infected strains are used to improve crop health and release mycoviruses into the field.

Insects have been on the menu of humans for centuries, but only recently we have begun to mass produce them for human food and animal feed. This introduction first paints a synopsis of mass cultured insects and their application. The new insect production industry raises many interesting fundamental and applied questions about insect biology and fitness. The second part of the introduction to this special issue addresses the 13 articles dealing with the improvement of mass-rearing efforts for a range of insects. The various studies focus on the effects of diet and microorganisms on relevant life-history traits and economic value of the insects. They reflect the current rapid developments in the insect production industry.

Insects have been on the menu of humans for centuries, but only recently we have begun to mass produce them for human food and animal feed. This introduction first paints a synopsis of mass cultured insects and their application. The new insect production industry raises many interesting fundamental and applied questions about insect biology and fitness. The second part of the introduction to this special issue addresses the 13 articles dealing with the improvement of mass-rearing efforts for a range of insects. The various studies focus on the effects of diet and microorganisms on relevant life-history traits and economic value of the insects. They reflect the current rapid developments in the insect production industry.

Area-wide integrated pest management strategies against tephritid fruit flies include the release of fruit fly parasitic wasps in the target area. Mass rearing of parasitic wasps is essential for the efficient application of biological control strategies. Enhancement of fruit fly host fitness through manipulation of their gut-associated symbionts might also enhance the fitness of the produced parasitic wasps and improve the parasitoid rearing system. In the current study, we added three gut bacterial isolates originating from Ceratitis capitata (Wiedemann) and four originating from Bactrocera oleae (Rossi) (both Diptera: Tephritidae) to the larval diet of C. capitata and used the bacteria-fed larvae as hosts for the development of the parasitic wasp Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae). We evaluated the effect of the bacteria on wasp life-history traits and assessed their potential use for the improvement of D. longicaudata rearing. Enterobactersp. AA26 increased fecundity and parasitism rate and accelerated parasitoid emergence. Providencia sp. AA31 led to faster emergence of both male and female parasitoids, whereas Providencia sp. 22 increased the production of female progeny. Bacillus sp. 139 increased parasitoid fecundity, parasitism rate, and production of female progeny. Serratia sp. 49 accelerated parasitoid emergence for both males and females and increased production of female progeny. Klebsiella oxytoca delayed parasitoid emergence and Enterobacter sp. 23 decreased parasitoid fecundity and parasitism rate. Our findings demonstrate a wide range of effects of fruit fly gut symbionts on parasitoid production and reveal a great potential of bacteria use towards enhancement of parasitic wasp rearing.

Botryosphaeria dothidea causes apple ring rot, which is among the most prevalent postharvest diseases of apples and causes significant economic loss during storage. In this study, we investigated the biocontrol activity and possible mechanism of Bacillus velezensis strain P2-1 isolated from apple branches against B. dothidea in postharvest apple fruit. The results showed strain P2-1, one of the 80 different endophytic bacterial strains from apple branches, exhibited strong inhibitory effects against B. dothidea growth and resulted in hyphal deformity. B. velezensis P2-1 treatment significantly reduced the ring rot caused by B. dothidea. Additionally, the supernatant of strain P2-1 exhibited antifungal activity against B. dothidea. Re-isolation assay indicated the capability of strain P2-1 to colonize and survive in apple fruit. PCR and qRT-PCR assays revealed that strain P2-1 harbored the gene clusters required for biosynthesis of antifungal lipopeptides and polyketides. Strain P2-1 treatment significantly enhanced the expression levels of pathogenesis-related genes (MdPR1 and MdPR5) but did not significantly affect apple fruit qualities (measured in fruit firmness, titratable acid, ascorbic acid, and soluble sugar). Thus, our results suggest that B. velezensis strain P2-1 is a biocontrol agent against B. dothidea-induced apple postharvest decay. It acts partially by inhibiting mycelial growth of B. dothidea, secreting antifungal substances, and inducing apple defense responses.

This work introduces an alternative workflow for the discovery of novel bacterial biocontrol agents in wheat against Fusarium head blight (FHB). Unlike the mass testing of isolate collections, we started from a diverse inoculum by extracting microbiomes from ears of field-grown plants at grain filling stage. Four distinct microbial communities were generated which were exposed to 3 14-day culture-independent experimental enrichments on detached wheat spikes infected with F. graminearum PH1. We found that one bacterial community reduced infection symptoms after 3 cycles, which was chosen to subsequently isolate bacteria through limiting dilution. All 94 isolates were tested in an in vitro and in planta assay, and a selection of 14 isolates was further tested on detached ears. The results seem to indicate that our enrichment approach resulted in bacteria with different modes-of-action in regard to FHB control. Erwinia persicina isolate C3 showed a significant reduction in disease severity (Fv/Fm), and Erwinia persicina C3 and Pseudomonas sp. B3 showed a significant reduction in fungal biomass (cGFP). However, the mycotoxin analysis of both these treatments showed no reduction in DON levels. Nevertheless, Pantoea ananatis H3 and H11 and Erwinia persicina H2 were able to reduce DON concentrations by more than 50%, although these effects were not statistically significant. Lastly, Erwinia persicina H2 also showed a significantly greater glucosylation of DON to the less phytotoxic DON-3G. The bacterial genera isolated through the enrichment cycles have been reported to dominate microbial communities that develop in open habitats, showing strong indications that the isolated bacteria can reduce the infection pressure of F. graminearum on the spike phyllosphere.

Simple Summary Turkey is the world's largest producer and exporter of hazelnut. Ambrosia beetle species are the most common species of pests for hazelnut in the orchards of Turkey. These beetles cause enormous economic losses by draining hazelnut branches and trees. The techniques for managing ambrosia beetles are limited. The more effective and eco-friendly alternative control methods, including the use of entomopathogenic fungi (EPF), should be included in integrated pest management programs to suppress ambrosia beetle populations. The objectives of the current study were (i) to isolate EPF from individual ambrosia beetles that were obtained from Turkey's main hazelnut production areas; (ii) to characterize EPF isolates using DNA sequencing and iPBS profiling; and (iii) to assess the effectiveness of the isolates against three ambrosia beetle species under laboratory conditions. A total of 47 EPF isolates were obtained from beetle cadavers and classified into eight EPF species. For the first time, the primer binding site (PBS) marker system was used to successfully discriminate among the EPF species. Some isolates caused 100% mortality of the beetle species within 7 to 9 days, depending on the beetle species, demonstrating their effectiveness in managing the pests. The major EPF species in this study provided an important basis for developing bioproducts and a possible alternative approach in controlling these ambrosia beetles. Ambrosia beetles, Anisandrus dispar Fabricius, Xylosandrus germanus Blandford, and Xyleborinus saxesenii Ratzeburg (Coleoptera: Curculionidae: Scolytinae) are among the most significant hazelnut pests in Turkey. The control of these pests is difficult and expensive due to their biology. The present study aimed to isolate entomopathogenic fungi (EPF) from A. dispar, X. germanus, and X. saxesenii individuals that were obtained from the main hazelnut production areas of Turkey, characterize the EPF isolates using internal transcribed spacer (ITS)-DNA sequencing and iPBS profiling, and determine the efficacy of the isolates against A. dispar, X. germanus, and X. saxesenii under laboratory conditions. Phylogenetic analyses based on ITS revealed that the 47 native isolates were Beauveria bassiana (11), B. pseudobassiana (8), Cordyceps fumosorosea (6), Cordyceps farinosa (1), Akanthomyces lecanii (13), Purpureocillium lilacinum (3), Clonostachys rosea (2) and Metarhizium anisopliae (3). For the first time, the primer binding site (PBS) marker system, based on retrotransposons, was used to discriminate successfully among the EPF species. Some isolates of B. bassiana, B. pseudobassiana, C. fumosorosea, A. lecanii, and M. anisopliae caused 100% mortality of the beetle species within 7 to 9 days. The findings of this study indicated that some isolated entomopathogenic fungi provide an essential basis for the development of bioproducts, as well as a promising alternative method for controlling these ambrosia beetles.

The predation rate and prey-stage preference of two mirid predatory bugs Macrolophus pygmaeus Rambur and Nesidiocoris tenuis (Reuter) (Hemiptera Miridae), were investigated on the diamondback moth (DBM) Plutella xylostella (L.) (Lepidoptera Plutellidae), a noxious pest of cruciferous plants. In particular, predation capacity of each predatory stages of both predators was studied on the first, second and third larval instars of DBM. There were no significant differences in predation between the predators on eggs, second and third instar larvae of DBM. Among the different life stages of both predators, females had the highest predation on eggs and all larval instars of DBM. Preference indices for both mirid species indicated that there was a preference toward eggs, first instar and second instar larvae versus first instar, second instar and third instar larvae, respectively. The data of the current study suggest that M. pygmaeus and N. tenuis are effective predators of DBM particularly against eggs and first instar larva. It would therefore be worth to investigate how these mirids can be incorporated in biological control programs of DBM.

Disease suppressive soils are important for managing soil-borne diseases that cannot be controlled with chemicals. One such disease is the potato common scab caused by Streptomyces species. Suppressiveness against common scab can develop spontaneously in fields where potato is grown for years without interruption, and this has been attributed to non-pathogenic Streptomyces strains. Streptomyces spp. have been used as inoculants in biological control, but their long-term effects have gained less attention. In our previous studies, a nonpathogenic Streptomyces strain (Str272) isolated from a potato common scab lesion suppressed common scab in field trials lasting over 5 years. In this study, bacterial communities in the tuberosphere i.e. in the soil adjacent to potato tubers, were analysed by next generation sequencing (NGS). The aim was to compare bacterial communities in untreated control plots to those in which seed tubers were treated with Str272 in one or several growing seasons. Str272 applications increased soil bacterial diversity and affected the bacterial composition in the potato tuberosphere. The most pronounced differences were observed between the untreated control and the treatments in which the antagonist had been applied in three or four consecutive years. The differences remained similar until the following growing season. Bacterial composition after repeated antagonist applications was associated with lower common scab severity. The antagonist applications had no or only slight effect on the number or abundance of OTUs belonging to Actinobacteria or Streptomyces, and no differences in quantities of pathogenic Streptomyces populations were detected by qPCR. This indicates that suppression of common scab by Str272 may not be based on direct effect on the common scab pathogens but is more likely to be associated with the alterations of the soil bacterial community. The most abundant bacteria phyla in the potato tuberosphere were Actinobacteria, Proteobacteria and Acidobacteria. However, the OTUs responding greatest to the antagonist treatments belonged to Bacterioidetes and Gemmatimonadetes. Results indicate that repeated applications of Str272 can change the bacterial community in the potato tuberosphere and lead to development of soil that is suppressive against potato common scab for several growing seasons after the last application.

Corn earworm, Helicoverpa zea (Boddie) (Lepidoptera: Noctuidae), has emerged as an injurious insect pest to hemp, Cannabis sativa L., a crop newly reintroduced to the United States. Growing hemp presents a potential alternative economic opportunity for farmers but can be challenging with a market that is unstable and just developing. One of the most notable production challenges is managing corn earworm, an insect pest that is particularly damaging when it feeds on flower buds produced in cannabinoid varieties, creating extensive bud tunneling and wounds that allow entry of pathogens that can aid development and presence of bud rot. Damage to seeds is of lesser concern in hemp cultivars grown for grain and minimal risk is associated with hemp grown for fiber. Our ability to research hemp has only recently been allowed as production was largely suspended following World War II and, as such, there has been limited opportunity to develop information for empirically-based pest management recommendations. Further complicating development of integrated pest management (IPM) strategies are regulatory challenges associated with providing registration support to add hemp to pesticide labels, as it was not formally recognized as a crop by U.S. regulatory agencies until late 2019. Research needs and challenges to develop effective IPM programs for corn earworm on hemp are discussed here.

Trihoderma citrinoviride protects plants from diseases by functioning as antagonists of many pathogenic fungi or by triggering the antioxidant defense system in plants. In the present study, to uncover the possible alleviative role of Trichoderma against salinity and Rhizoctonia solani infection, strawberry plants were pretreated Trichoderma citrinoviride and then subjected to salinity, R. solani and combined salinity and R. solani. The effect of T. citrinoviride on the alleviation of the effects of salt stress and Rhizoctonia solani infection was investigated by analysing leaf dry weight, PSII efficiency, and the activity of some antioxidant enzymes in the leaves of strawberry plants. T. citrinoviride improved competitive capability against salinity and R. solani infection. It showed 79% inhibition of the growth of pathogen R. solani. T. citrinoviride reduced 63% of the severity of disease in the leaves. Trichoderma pretreatment maximized plant dry weight. The T. citrinoviride-pretreated plants showed higher levels of PSII efficiency (Fv/Fm). Decreased lipid peroxidation and H2O2 accumulation compared to untreated seedlings under salt stress and R. solani infection was observed. Trichoderma-pretreated and –untreated plants respond differently to salt stress and R. solani infection by means of antioxidant defense. As compared to untreated seedlings, treated seedlings showed significantly lower activities of antioxidant enzymes, superoxide dismutase (SOD), peroxidase (POX), cell wall peroxidase (CWPOX) under salt stress and R. solani infection, indicating that treated seedlings might sense lower stress as compared to untreated seedlings. The study reports the effective adaptive strategy and potential of T. citrinoviride in alleviating the negative impact of salt stress and R. solani infection in strawberry.

There is growing evidence about the role of insectivorous bats against agricultural pests in various crops. Nevertheless, little research addressed the aggregational and functional responses of bat assemblages to changes in pest availability across a spatio-temporal scale. Therefore, we examined the activity and diet habits of different bat species using DNA metabarcoding by simultaneously monitoring the relative abundance of two major pests (the European grapevine moth, Lobesia botrana, and the leaf rolling tortrix, Sparganothis pilleriana) through the grape growing season, in a vineyard region of the Iberian Peninsula. During pest major irruptions, we found the highest bat activity levels and frequencies of grape pests in the diet of bats, although not all bat species contributed equally to pest suppression. Bats of different foraging guilds positively responded to pest abundances, indicating distinct bat species may synergistically play a role at suppressing agricultural pests at broad scales of the aerospace. For instance, narrow space foragers exploiting major irruptions in grape interior, edge space foragers hampering pest dispersion at local scale, and open space foragers preventing infestations of new grapevine patches at broader scales. Yet, our study exposed the current methodological constraints regarding pest dispersion dynamics, acoustic monitoring of bats? foraging activity or the unfeasibility of metabarcoding to reliably quantify prey abundance in bats diet, and thus further improvement in these issues is required in order to gain insight on the agroecological interactions between bats and pests. We are grateful to owners of the winery cellars (Artadi, Berarte, Laukote, Puelles, Ramon Saenz, Granja Nuestra Senora de Remelluri and R. Lopez de Heredia Vina Tondonia) for facilitating the collection of faeces and for allowing us to conduct the experiments on their estates. We also thank Ana Maria Diez Navajas and NEIKER-Tecnalia workers for sending data on the phenology of L. botrana during the sampling period. Special gratitude to Gonzalo Garcia Baquero for his valuable help with statistical analysis and Vanessa A. Mata for her priceless advice on modeling and the Sequencing and Genotyping Unit-Genomic Facilities-SGIker (UPV/EHU/ERDF, EU) for the technical support provided. This project was funded by the Spanish Ministry of Economy and Competitiveness and the European Regional Development Fund (CGL-2015-69069P), as well as the Government of the Basque Country (IT75413 and IT1163-19). The Basque Government granted UB (PRE_2019_2_0186).

The antagonistic activities of native Debaryomyces hansenii strains isolated from Danish cheese brines were evaluated against contaminating molds in the dairy industry. Determination of chromosome polymorphism by use of pulsed-field gel electrophoresis (PFGE) revealed a huge genetic heterogeneity among the D. hansenii strains, which was reflected in intra-species variation at the phenotypic level. 11 D. hansenii strains were tested for their ability to inhibit germination and growth of contaminating molds, frequently occurring at Danish dairies, i.e., Cladosporium inversicolor, Cladosporium sinuosum, Fusarium avenaceum, Mucor racemosus, and Penicillium roqueforti. Especially the germination of C. inversicolor and P. roqueforti was significantly inhibited by cell-free supernatants of all D. hansenii strains. The underlying factors behind the inhibitory effects of the D. hansenii cell-free supernatants were investigated. Based on dynamic headspace sampling followed by gas chromatography-mass spectrometry (DHS-GC-MS), 71 volatile compounds (VOCs) produced by the D. hansenii strains were identified, including 6 acids, 22 alcohols, 15 aldehydes, 3 benzene derivatives, 8 esters, 3 heterocyclic compounds, 12 ketones, and 2 phenols. Among the 71 identified VOCs, inhibition of germination of C. inversicolor correlated strongly with three VOCs, i.e., 3-methylbutanoic acid, 2-pentanone as well as acetic acid. For P. roqueforti, two VOCs correlated with inhibition of germination, i.e., acetone and 2-phenylethanol, of which the latter also correlated strongly with inhibition of mycelium growth. Low half-maximal inhibitory concentrations (IC50) were especially observed for 3-methylbutanoic acid, i.e., 6.32–9.53 × 10–5 and 2.00–2.67 × 10–4 mol/L for C. inversicolor and P. roqueforti, respectively. For 2-phenylethanol, a well-known quorum sensing molecule, the IC50 was 1.99–7.49 × 10–3 and 1.73–3.45 × 10–3 mol/L for C. inversicolor and P. roqueforti, respectively. For acetic acid, the IC50 was 1.35–2.47 × 10–3 and 1.19–2.80 × 10–3 mol/L for C. inversicolor and P. roqueforti, respectively. Finally, relative weak inhibition was observed for 2-pentanone and acetone. The current study shows that native strains of D. hansenii isolated from Danish brines have antagonistic effects against specific contaminating molds and points to the development of D. hansenii strains as bioprotective cultures, targeting cheese brines and cheese surfaces.

The pine bark adelgid, Pineus strobi (Hartig) (Hemiptera: Adelgidae), is an herbivore native to eastern North America that specialises on eastern white pine, Pinus strobus Linnaeus (Pinaceae). Little is known about P. strobi, especially in its southern range in the Appalachian Mountains, United States of America, and the composition of its predator complex has not yet been documented in this region. The current study identifies arthropod predators associated with P. strobi in Appalachian forests of Virginia based on a two-year survey. Predators were identified using morphology and DNA barcoding. Predator species include: Laricobius rubidus LeConte (Coleoptera: Derodontidae), Leucopis piniperda Malloch (Diptera: Chamaemyiidae), and Leucopis argenticollis Zetterstedt (Diptera: Chamaemyiidae), that are known adelgid specialists. Also found were predators from the families Cecidomyiidae (Diptera), Coccinellidae (Coleoptera), Chrysopidae (Neuroptera), Hemerobiidae (Neuroptera), and Syrphidae (Diptera). The Cecidomyiidae were especially diverse, with 14 different species inferred from their DNA barcodes. Knowledge of this predator complex is particularly valuable for anticipation and detection of potential interactions between native predator species and those that are being considered for the introduction for biological control of invasive adelgid pests within the southern Appalachian ecosystem.

Fusarium basal rot (FBR) is a soil-borne disease that affects Allium species worldwide. Although FBR has long been recognized as a major constraint to the production of economically important Allium species, information that could support disease management remains scattered. In this review, the current knowledge on the causal agents, symptomology and epidemiology, impact, and management strategies of FBR is synthesized. We highlight that FPR is associated with different complexes of several Fusarium species, of which Fusarium oxysporum and F. proliferatum are the most prevalent. These pathogenic complexes vary in composition and virulence, depending on sites and hosts, which can be challenging for disease management. Research to improve disease management using chemical pesticides, resistance cultivars, biocontrol agents, and cultural practices has achieved both promising results and limitations. Finally, research needs and future directions are proposed for the development of effective FBR management strategies.

Biochar has been reported to play a positive role in disease suppression against airborne pathogens in plants. The mechanisms behind this positive trait are not well-understood. In this study, we hypothesized that the attraction of plant growth-promoting rhizobacteria (PGPR) or fungi (PGPF) underlies the mechanism of biochar in plant protection. The attraction of PGPR and PGPF may either activate the innate immune system of plants or help the plants with nutrient uptake. We studied the effect of biochar in peat substrate (PS) on the susceptibility of strawberry, both on leaves and fruits, against the airborne fungal pathogen Botrytis cinerea. Biochar had a positive impact on the resistance of strawberry fruits but not the plant leaves. On leaves, the infection was more severe compared with plants without biochar in the PS. The different effects on fruits and plant leaves may indicate a trade-off between plant parts. Future studies should focus on monitoring gene expression and metabolites of strawberry fruits to investigate this potential trade-off effect. A change in the microbial community in the rhizosphere was also observed, with increased fungal diversity and higher abundances of amplicon sequence variants classified into Granulicella, Mucilaginibacter, and Byssochlamys surrounding the plant root, where the latter two were reported as biocontrol agents. The change in the microbial community was not correlated with a change in nutrient uptake by the plant in either the leaves or the fruits. A decrease in the defense gene expression in the leaves was observed. In conclusion, the decreased infection of B. cinerea in strawberry fruits mediated by the addition of biochar in the PS is most likely regulated by the changes in the microbial community.

Entomopathogenic nematodes (EPNs) in the families Steinernematidae and Heterorhabditidae are effective biocontrol agents against a number of important soil insect pests and are safe to vertebrates, plants and other non-target organisms with no negative effects on the environment. They are associated with mutualistic bacteria in the genus Xenorhabdus for Steinernematidae and Photorhabdus for Heterorhabditidae and are often referred to as the nematode/bacterium complex. The natural habitat of EPNs is the soil where the third-stage infective juveniles (IJs), the only free-living stage, can infect an array of insect hosts. However, the survival of the IJs is affected by various abiotic (i.e., temperature, moisture, soil texture, soil salinity, UV light, oxygen, and pH) and biotic (i.e., natural enemies, omnivores, scavengers, competitors, and plants) factors in the soil. In this review, we summarize the impact of the biotic factors, especially natural enemies, omnivores, scavengers, and competitors against EPNs and conclude that the major predators of the IJs appear to be nematophagous fungi, mites, and collembolans. Omnivores and scavengers, particularly, mites, collembolans, ants and birds, feeding on EPNkilled insects appear to be the major mortality factors on the developing nematodes especially of insects that are 2 days or less post infection. In addition, we discuss the defense mechanisms of EPN IJs against nematophagous fungi, and the protection of the nematode-killed insects by production of the scavenger deterrent factor produced by the mutualistic bacteria against omnivores and scavengers. By understanding the interactions between EPNs and their antagonists we can improve and increase the successful use of EPNs in biological control programs. Aydin Adnan Menderes University Research Fund, Turkey [FEF-14006]; Scientific and Technical Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [116Z074]; RUSA -Phase 2.0 grant [Policy (TN Multi-Gen), Dept. of Edn. Govt. of India] [F.24-51/2014-U]; University Science Instrumentation Centre (USIC), Alagappa University, Karaikudi, Tamil Nadu, India The study was supported by Aydin Adnan Menderes University Research Fund, Turkey (Project number: FEF-14006); Scientific and Technical Research Council of Turkey (TUBITAK-Project Number: 116Z074); RUSA -Phase 2.0 grant [Letter No. F.24-51/2014-U, Policy (TN Multi-Gen), Dept. of Edn. Govt. of India, Dt. 09.10.2018] and University Science Instrumentation Centre (USIC), Alagappa University, Karaikudi, Tamil Nadu, India. WOS:000597333300003 2-s2.0-85092116227

In this study, which was conducted to determine predation potential of Typhlodromus recki Wainstein, 1958 (Acari: Phytoseiidae) at Ege University, Faculty of Agriculture, Department of Plant Protection in 2018-2019. Functional response and egg production of the predatory mite, T. recki fed on different biological stages (egg, larva, protonymph, deutonymph and adult male) of the two-spotted spider mite (green form), Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) were studied under laboratory conditions (25 +/- 1 degrees C, 60 +/- 10% RH and 16:8 h L:D photoperiod). In the experiments, seven prey densities (2, 4, 8, 16, 32, 64 and 128) for each biological stage of the prey were offered daily to the predatory mite. The results of logistic regression analysis indicated that T. recki had a Type II functional response on each developmental stage of its prey according to Holling's models. The attack rate (alpha) and the handling time (T-h) varied based on the biological stages of the prey. The highest alpha and the lowest T-h values were determined as 1.035 and 0.001 when the predator fed on larvae and eggs of its prey, respectively. The highest average daily mean number of the eggs consumed by T. recki was 111 at 128 prey densities. The highest average daily mean number of eggs deposited by the predator were found to be 1.05 when it fed on the eight-prey density of T. urticae protonymphs. In addition, the lowest average daily mean number of eggs deposited by the predator was 0.15 when fed on the two-prey density with T. urticae adult males. The study indicates that T. recki could be effective and promising biological control agent for T. urticae., Scientific and Technological Research Council of Turkey [118O666], This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK-Project Number: 118O666) . I am grateful to Dr. Ibrahim Cakmak (Aydn Adnan Menderes University, Faculty of Agriculture, Department of Plant Protection, Aydn, Turkey) and Dr. smail Doker (Cukurova University, Faculty of Agriculture, Department of Plant Protection, Adana, Turkey) for data analyses and editing the manuscript.

An infective prey has the potential to infect, kill and consume its predator. Such a prey-predator relationship fundamentally differs from the predator-prey interaction because the prey can directly profit from the predator as a growth resource. Here we present a population dynamics model of partial role reversal in the predator-prey interaction of two species, the bottom dwelling marine deposit feeder sea cucumber Apostichopus japonicus and an important food source for the sea cucumber but potentially infective bacterium Vibrio splendidus. We analyse the effects of different parameters, e.g. infectivity and grazing rate, on the population sizes. We show that relative population sizes of the sea cucumber and V. Splendidus may switch with increasing infectivity. We also show that in the partial role reversal interaction the infective prey may benefit from the presence of the predator such that the population size may exceed the value of the carrying capacity of the prey in the absence of the predator. We also analysed the conditions for species extinction. The extinction of the prey, V. splendidus, may occur when its growth rate is low, or in the absence of infectivity. The extinction of the predator, A. japonicus, may follow if either the infectivity of the prey is high or a moderately infective prey is abundant. We conclude that partial role reversal is an undervalued subject in predator-prey studies.

Feeding a rising population of currently 7.8 billion people globally requires efficient agriculture, which is preferably sustainable. Today, farmers are largely dependent on synthetic fungicides to avoid food losses caused by fungal diseases. However, the extensive use of these has resulted in the emergence of fungicide-resistant pathogens and concerns have been raised over the residual effects on the environment and human health. In this regard, biocontrol agents (BCAs) have been proposed as an alternative to standard fungicides but their disease management capacity is usually incomplete and heavily relies on uncontrollable environmental conditions. An integrated approach combining BCAs with fungicides, which is the focus of this review, is put forward as a way to reduce the fungicide doses to manage plant diseases and thereby their residue on harvested crops. In addition, such a strategy of combining antifungal treatments with different modes of action reduces the selection pressure on pathogens and thereby the chances of resistance development. However, to allow its large-scale implementation, further knowledge is needed, comprising timing, number and interval of repeated BCA applications and their compatibility with fungicides. The compatibility of BCAs with fungicides might differ when applied in a mixture or when used in alternation. ispartof: MICROORGANISMS vol:8 issue:12 ispartof: location:Switzerland status: published

Harmonia axyridis is an invasive alien ladybird in North America and Europe. Studies show that multiple natural enemies are using Ha. axyridis as a new host. However, thus far, no research has been undertaken to study the effects of simultaneous infections of multiple natural enemies on Ha. axyridis. We hypothesized that high thallus densities of the ectoparasitic fungus Hesperomyces virescens on a ladybird weaken the host’s defenses, thereby making it more susceptible to infection by other natural enemies. We examined mortality of the North American-native Olla v-nigrum and Ha. axyridis co-infected with He. virescens and an entomopathogenic fungus—either Beauveria bassiana or Metarhizium anisopliae. Laboratory assays revealed that He. virescens-infected O. v-nigrum individuals are more susceptible to entomopathogenic fungi, but Ha. axyridis does not suffer the same effects. This is in line with the enemy release hypothesis, which predicts that invasive alien species in new geographic areas experience reduced regulatory effects from natural enemies compared to native species. Considering our results, we can ask how He. virescens affects survival when confronted by other pathogens that previously had little impact on Ha. axyridis.

Since its introduction to control overabundant invasive European rabbits (Oryctolagus cuniculus), the highly virulent rabbit haemorrhagic disease virus (RHDV) has caused regular annual disease outbreaks in Australian rabbit populations. Although initially reducing rabbit abundance by 60%, continent���wide, experimental evidence has since indicated increased genetic resistance in wild rabbits that have experienced RHDV���driven selection. To identify genetic adaptations, which explain the increased resistance to this biocontrol virus, we investigated genome���wide SNP (single nucleotide polymorphism) allele frequency changes in a South Australian rabbit population that was sampled in 1996 (pre���RHD genomes) and after 16 years of RHDV outbreaks. We identified several SNPs with changed allele frequencies within or close to genes potentially important for increased RHD resistance. The identified genes are known to be involved in virus infections and immune reactions or had previously been identified as being differentially expressed in healthy versus acutely RHDV���infected rabbits. Furthermore, we show in a simulation study that the allele/genotype frequency changes cannot be explained by drift alone and that several candidate genes had also been identified as being associated with surviving RHD in a different Australian rabbit population. Our unique data set allowed us to identify candidate genes for RHDV resistance that have evolved under natural conditions, and over a time span that would not have been feasible in an experimental setting. Moreover, it provides a rare example of host genetic adaptations to virus���driven selection in response to a suddenly emerging infectious disease., publishedVersion

We studied the food quality of the aphid Rhopalosiphum padi to the pirate bug Orius majusculus using Ephestia eggs as high-quality comparison prey. Several performance parameters were tested on individuals that had been reared and maintained on each of the two single-prey diets or on a mixed diet. All fitness parameters were lower in individuals fed aphids only, indicating poor food quality of this prey. Compared with the pure Ephestia egg diet, the mixed diet enhanced teneral mass, while adult survival and female starvation tolerance were negatively affected and all other traits were unaffected. Body protein proportions were constant across diets, whereas lipid proportion was low in the aphid treatment. Preference for aphids was lower following a monotypic aphid diet than when reared on Ephestia eggs or a mixed diet. The results confirm that R. padi is low-quality food for O. majusculus as it is for other generalist predators, even though O. majusculus may contribute significantly to population suppression of the aphid.

Sphecophaga vesparum often parasitizes nests of vespid wasps such as Vespula vulgaris and Vespula germanica. Inside the colonies, the ectoparasitic larvae feed on the immature forms of the wasps. There are two adult forms of S. vesparum. The large, winged adults emerge from either rigid yellow cocoons or the orange cocoons used for overwintering. The small, brachypterous females emerge from soft, white cocoons. The species is facultative deuterotokous, producing mostly parthenogenic females and infrequently producing males. Here, we describe the production of chemical compounds related to the different developmental forms of the parasitoid S. vesparum (larvae, pupae and adults). We also compare the chemical profiles of the parasitoid wasp adults to those of their two main host species, Vespula vulgaris and Vespula germanica. The results show differences in hydrocarbon composition of larvae, pupae and adults of S. vesparum. Our results also suggest a partial mimicry of each of the two host species, mostly relating to linear alkanes present in both parasitoids and the host vespid wasp species. This matching is likely due to the recycling of the prey&rsquo, s hydrocarbons, as has been found in other species of parasitoids.

Rhizocephalans are abundant members of marine ecosystems and are important regulators of crustacean host populations. Morphological and ecological variation makes them an attractive system for evolutionary studies of advanced parasitism. Such studies have been impeded by a largely formalistic taxonomy, because rhizocephalan morphology offers no characters for a robust phylogenetic analysis. We use DNA sequence data to estimate a new phylogeny for 43 species and use this to develop a revised taxonomy for all Rhizocephala. Our taxonomy accepts 13 new or redefined monophyletic families. The traditional subdivision into the suborders Kentrogonida and Akentrogonida is abandoned, because both are polyphyletic. The three ‘classical’ kentrogonid families are also polyphyletic, including the species-rich Sacculinidae, which is split into a redefined and a new family. Most species of large families remain to be studied based on molecular evidence and are therefore still assigned to their current genus and family by default. We caution against undue generalizations from studies on model species until a more stable species-level taxonomy is also available, which requires more extensive genus- and species-level sampling with molecular tools. We briefly discuss the most promising future studies that will be facilitated by this new phylogeny-based taxonomy.

Alfalfa weevil (Hypera postica Gyllenhal, 1813) samples were collected from different locations in Kirsehir, Turkey, during May-August in 2014. They were immediately transferred to the research laboratory and dissected under aseptic conditions. Then serial dilutions were prepared from digestive tract samples and the spread plate technique was used for obtaining pure cultures. The conventional and molecular methods that include morphological and physiological examinations, biochemical tests, 16S rDNA sequencing, and the basic local alignment search tool (BLAST) were used for identification of the bacterial isolates. According to the findings of the present study, the YHH-2 isolate was found as the main pathogenic bacterium that dominates the digestive tract microflora of the alfalfa weevil. 16S rDNA sequencing and the BLAST data revealed that YHH-2 showed 100% similarity to Bacillus pumilus, recently described as an entomopathogenic strain. In conclusion, the results of the present study are important due to showing for the first time the presence of Bacillus pumilus Y HH-2 in the digestive tract of the alfalfa weevil and it may be used as a potential biocontrol agent against the harmful effects of this insect species., Ahi Evran University Research Fund [4003/2.13.002, 201, 2013], This work was supported by the Ahi Evran University Research Fund (Project Number: 4003/2.13.002, 201, 2013).

New genetic control methods for mosquitoes may reduce vector species without direct effects on other species or the physical environment common with insecticides or drainage. Effects on predators and competitors could, however, be a concern as Anopheles gambiae s.l. is preyed upon in all life stages. We overview the literature and assess the strength of the ecological interactions identified. Most predators identified consume many other insect species and there is no evidence that any species preys exclusively on any anopheline mosquito. There is one predatory species with a specialisation on blood‐fed mosquitoes including An. gambiae s.l.. Evarcha culicivora is a jumping spider, known as the vampire spider, found around Lake Victoria. There is no evidence that these salticids require Anopheles mosquitoes and will readily consume blood‐fed Culex. Interspecific competition studies focus on other mosquitoes of larval habitats. Many of these take place in artificial cosms and give contrasting results to semi‐field studies. This may limit their extrapolation regarding the potential impact of reduced An. gambiae numbers. Previous mosquito control interventions are informative and identify competitive release and niche opportunism; so while the identity and relative abundance of the species present may change, the biomass available to predators may not.

Endophytic bacteria (EB) are ubiquitous in most plant species and they colonize plant tissues actively and systemically. Their colonization and persistence in the plant may be crucial to plant growth, yield and suppressing diseases. In this study, the long-term population dynamics of the two EB, namely Ochrobactrum spp. strain CB36/1 and Pantoea agglomerans strain CC37/2 in cucumber plants (Cucumis sativus L. cv. Gordion F1), were monitored. Also, the potential effects of these EB on plant growth, fruit quality, and yield, as well as the influences on Angular leaf spot disease of cucumber (ALS) and the yield under the disease pressure, were investigated. Plants were grown using the soilless cultivation technique in the greenhouse. Inoculation of the EB took place twice, and their population in plant tissues were monitored periodically for 62 days. Both EB isolates survived in plant organs until the end of the growing season, but over time, population densities dropped from 105 to 103 CFU g. plant?1 with the age of the plant. The EB applications had significant effects on fruit length, color and firmness. Total yield increased by 22% with CB36/1 and 21% with CC37/2 without disease pressure. Only CC37 / 2 significantly reduced the severity of ALS disease by 41% and increased yield by 22% compared to pathogen treatment alone. In this study, it was observed that the EB strain CC37/2 might contribute to reducing the chemical input and prevent the yield losses in soilless growing systems within the integrated agricultural concept. © 2021 Elsevier B.V., FA1103 Türkiye Bilimsel ve Teknolojik Araştirma Kurumu, TÜBITAK: TUBITAK-COST 111 O 505, The present study was sponsored by national grants from the Scientific and Technological Research Council of Turkey (TUBITAK-COST 111 O 505) and Ege University Science-Technology Research and Application Center (EBILTEM). The authors express their gratitude for the contribution of the participants in EU-COST Action FA1103., The present study was sponsored by national grants from the Scientific and Technological Research Council of Turkey ( TUBITAK-COST 111 O 505 ) and Ege University Science-Technology Research and Application Center ( EBILTEM ). The authors express their gratitude for the contribution of the participants in EU-COST Action FA1103.

Plant root secreted compounds alter the gene expression of associated microorganisms by acting as signal molecules that either stimulate or repel the interaction with beneficial or harmful species, respectively. However, it is still unclear whether two distinct groups of beneficial bacteria, non-plant-associated (soil) strains and plant-associated (endophytic) strains, respond uniformly or variably to the exposure with root exudates. Therefore, Bacillus mycoides, a potential biocontrol agent and plant growth-promoting bacterium, was isolated from the endosphere of potatoes and from soil of the same geographical region. Confocal fluorescence microscopy of plants inoculated with GFP-tagged B. mycoides strains showed that the endosphere isolate EC18 had a stronger plant colonization ability and competed more successfully for the colonization sites than the soil isolate SB8. To dissect these phenotypic differences, the genomes of the two strains were sequenced and the transcriptome response to potato root exudates was compared. The global transcriptome profiles evidenced that the endophytic isolate responded more pronounced than the soil-derived isolate and a higher number of significant differentially expressed genes were detected. Both isolates responded with the alteration of expression of an overlapping set of genes, which had previously been reported to be involved in plant-microbe interactions; including organic substance metabolism, oxidative reduction, and transmembrane transport. Notably, several genes were specifically upregulated in the endosphere isolate EC18, while being oppositely downregulated in the soil isolate SB8. These genes mainly encoded membrane proteins, transcriptional regulators or were involved in amino acid metabolism and biosynthesis. By contrast, several genes upregulated in the soil isolate SB8 and downregulated in the endosphere isolate EC18 were related to sugar transport, which might coincide with the different nutrient availability in the two environments. Altogether, the presented transcriptome profiles provide highly improved insights into the life strategies of plant-associated endophytes and soil isolates of B. mycoides.