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The present research work was carried out in 6 milking parlors of the Cordillera Department. The objective of the research was to determine the sensitivity to antimicrobials of Streptococcus spp. from milk samples from lactating cows positive to California Mastitis Test in the dairy farms mentioned in 2018. For this purpose, 200 bovine were counted, of milk aptitude, in lactation, without distinction of race or age, of which 141 (70,5%) were positive to the California Mastitis Test (CMT). The positive samples were identified, refrigerated and sent to the laboratory of the Department of Microbiology and Immunology of the Faculty of Veterinary Sciences of the National University of Asunción, where the corresponding microbiological analyzes were performed. The laboratory results obtained indicated that of the 141 samples analyzed in 89 (63,12%), bacterial microorganisms were isolated; 26 (29,21%) corresponding to Streptococcus spp.; of which 2 (7.69%) were sensitive to oxaciline; 19 (73,08%) to cefotaxime; 26 (100%) to penicillin; 22 (84,61%) to erythomycin; 25 (96,15%) to clindamycin; 24 (92,30%) to chloramphenicol; 15 (57,69%) to tetracycline; 22 (84,61%) at teicoplanine and 25 (96,15%) were sensitive to rifampicin. [ABSTRACT FROM AUTHOR]

The aim of our paper is to present a case study carried out with a Translation and Interpreting student with Asperger's Syndrome (AS) in the framework of the e-learning project "TRADICOR: Corpora Management System for the Teaching Innovation in Translation and Interpreting" (PIE13-054). Our hypothesis is the following: when students with AS translate texts, simplification at two levels (text simplification and instructions simplifications) represents an improvement on translation quality and entails a better understanding of instructions. To confirm this hypothesis, the following four goals have been established: 1) apply text simplification to instructions in Spanish and to source texts in Spanish using Anula's recommendations; 2) checking the readability of the Spanish simplified and non-simplified source texts and instructions using two Spanish formulae: the Flesch-Szigriszt Index and the Fernández Huerta Index; 3) assessing target texts using Toledo Báez's analytic assessment template in order to check whether text simplification implies an improvement on translation quality; 4) test whether the Toledo Báez, M. Cristina (2018). Text Simplification in a Case Study with a Translation Student with Asperger's Syndrome. [ABSTRACT FROM AUTHOR]

One might expect that tax benefits introduced by certain developed countries to attract foreign high-skilled workers would run contrary to taxes aimed at the alleviation or deterrence of so-called brain drain. This article shows, however, that this is not necessarily the case: brain gain benefits might be justified by the existence of home-state brain drain taxes and, at the same time, serve to alleviate international double taxation generated by this type of taxes.

The establishment of successful interspecies hybrids requires restoration of a stable "2n" chromosome complement which can produce viable "n" gametes. This may occur (rarely) via recombination between non-homologous chromosomes, or more commonly is associated with a doubling of parental chromosome number to produce new homologous pairing partners in the hybrid. The production of unreduced "2n" gametes (gametes with the somatic chromosome number) may therefore be evolutionarily useful by serving as a key pathway for the formation of new polyploid hybrids, as might specific mechanisms permitting recombination between non-homologous chromosomes. Here, we investigated chromosome complements and fertility in third generation interspecific hybrids (AABC) resulting from a cross between allopolyploids Brassica juncea (AABB) × B. napus (AACC) followed by self-pollination for two generations. Chromosome numbers ranged from 2n = 48-74 in the experimental population (35 plants), with 9-16 B genome chromosomes and up to 4 copies of A genome chromosomes. Unreduced gamete production leading to a putative genome structure of approximately AAAABBCC was hence predicted to explain the high chromosome numbers observed. Additionally, the estimation of nuclei number in post-meiotic sporads revealed a higher frequency of unreduced gametes (0.04-5.21%) in the third generation AABC interspecific hybrids compared to the parental Brassica juncea (0.07%) and B. napus (0.13%). Our results suggest that unreduced gamete production in the subsequent generations following interspecific hybridization events may play a critical role in restoration of more stable, fertile chromosome complements., Competing Interests: Competing interests: The authors declare no competing interests., (© 2024. The Author(s).)

The increase in the global prevalence of type 2 diabetes mellitus (DM2), driven mainly by obesity and physical inactivity, has increased interest in various nonpharmacological therapies. This systematic review aims to establish the effectiveness of high-intensity interval training (HIIT) and resistance exercise (RE) compared with continuous aerobic exercise in improving control in patients with DM2. We conducted a comprehensive search for clinical trials using databases such as MEDLINE (PubMed) and Web of Science. The search was performed using a controlled vocabulary (MeSH) together with Boolean operators, and the results were limited to English and Spanish. Secondary outcomes were improvements in VO 2 max and decreases in low-density lipoprotein (LDL). This study aims to explain evidence-based recommendations for primary care physicians on exercise therapies to improve glycemic management as well as cardiovascular health in people with DM2., Competing Interests: Conflicts of interest: In compliance with the ICMJE uniform disclosure form, all authors declare the following: Payment/services info: All authors have declared that no financial support was received from any organization for the submitted work. Financial relationships: All authors have declared that they have no financial relationships at present or within the previous three years with any organizations that might have an interest in the submitted work. Other relationships: All authors have declared that there are no other relationships or activities that could appear to have influenced the submitted work., (Copyright © 2024, Muñoz Rossi et al.)

Juncus is the largest genus of Juncaceae and was considered holocentric for a long time. Recent findings, however, indicated that 11 species from different clades of the genus have monocentric chromosomes. Thus, the Juncus centromere organization and evolution need to be reassessed. We aimed to investigate the major repetitive DNA sequences of two accessions of Juncus effusus and its centromeric structure by employing whole-genome analyses, fluorescent in situ hybridization, CENH3 immunodetection, and chromatin immunoprecipitation sequencing. We showed that the repetitive fraction of the small J. effusus genome (~270 Mbp/1C) is mainly composed of Class I and Class II transposable elements (TEs) and satellite DNAs. Three identified satellite DNA families were mainly (peri)centromeric, with two being associated with the centromeric protein CENH3, but not strictly centromeric. Two types of centromere organization were discerned in J. effusus: type 1 was characterized by a single CENH3 domain enriched with JefSAT1-155 or JefSAT2-180, whereas type 2 showed multiple CENH3 domains interrupted by other satellites, TEs or genes. Furthermore, while type 1 centromeres showed a higher degree of satellite identity along the array, type 2 centromeres had less homogenized arrays along the multiple CENH3 domains per chromosome. Although the analyses confirmed the monocentric organization of J. effusus chromosomes, our data indicate a more dynamic arrangement of J. effusus centromeres than observed for other plant species, suggesting it may constitute a transient state between mono- and holocentricity., (© 2024 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd.)

The increasing demand for optimizing the use of agricultural resources will require the adoption of cutting-edge technologies and precision farming management. Unmanned Aerial Vehicle (UAV) sprayers seem promising due to their potential to perform precision or spot spraying, particularly in woody crop environments where total surface spraying is unnecessary. However, incorporating this technology is limited by the lack of scientific knowledge about the environmental risks associated with UAV sprayers and the strict legal framework. Nonetheless, these spraying systems' characteristic downwash airflow and the limited swath width can potentially mitigate drift in hedgerow crops. During our study we performed comparative studies aimed to compare the airborne drift, soil, and crop depositions between a conventional orchard sprayer and a UAV sprayer in a commercial superhigh-density orchard in the South Iberian Peninsula in 2022. Our findings reveal that, in superhigh-density olive orchards, the UAV sprayer presents a substantial reduction in airborne drift, while soil depositions showed no significant differences compared to those of a conventional terrestrial orchard sprayer. Crop depositions were significantly lower when utilizing the UAV sprayer. These results suggest that introducing UAV spraying technology in Mediterranean agricultural systems, under specific scenarios, can effectively reduce the environmental impact of crop spraying and encourage the responsible use of plant protection products (PPPs)., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Sánchez-Fernández, Barrera-Báez, Martínez-Guanter and Pérez-Ruiz.)

Glucokinase (GCK) is the pancreatic β-cell glucose sensor, and its kinetics are key to that purpose. A slow transition step, displayed as non-hyperbolic kinetics, and a low affinity for glucose characterize GCK. Mutations in GCK associated with maturity-onset diabetes of the young type 2 (MODY2) previously described reduce the functionality of the human pancreatic β-cell, leading to diabetic clinical phenotypes. We present a kinetic characterization of the G448D mutation identified in a MODY2 patient, which is one of the first mutations to exhibit increased functionality. This mutant displays increased activity, high affinity for both Mg 2+ -ATP and glucose, hyperbolic kinetics and increased phosphorylation potential. Hyperbolic kinetics and assays in the presence of glycerol indicate that G448D lacks the slow transition step crucial for the pancreatic β-cell glucose sensor function., (© 2022 Federation of European Biochemical Societies.)

The genus Agave presents a bimodal karyotype with x = 30 (5L, large, +25S, small chromosomes). Bimodality within this genus is generally attributed to allopolyploidy in the ancestral form of Agavoideae. However, alternative mechanisms, such as the preferential accumulation of repetitive elements at the macrochromosomes, could also be important. Aiming to understand the role of repetitive DNA within the bimodal karyotype of Agave , genomic DNA from the commercial hybrid 11648 (2 n = 2 x = 60, 6.31 Gbp) was sequenced at low coverage, and the repetitive fraction was characterized. In silico analysis showed that ~67.6% of the genome is mainly composed of different LTR retrotransposon lineages and one satellite DNA family (AgSAT171). The satellite DNA localized at the centromeric regions of all chromosomes; however, stronger signals were observed for 20 of the macro- and microchromosomes. All transposable elements showed a dispersed distribution, but not uniform across the length of the chromosomes. Different distribution patterns were observed for different TE lineages, with larger accumulation at the macrochromosomes. The data indicate the differential accumulation of LTR retrotransposon lineages at the macrochromosomes, probably contributing to the bimodality. Nevertheless, the differential accumulation of the satDNA in one group of macro- and microchromosomes possibly reflects the hybrid origin of this Agave accession.

Cryptography has become one of the vital disciplines for information technology such as IoT (Internet Of Things), IIoT (Industrial Internet Of Things), I4.0 (Industry 4.0), and automotive applications. Some fundamental characteristics required for these applications are confidentiality, authentication, integrity, and nonrepudiation, which can be achieved using hash functions. A cryptographic hash function that provides a higher level of security is SHA-3. However, in real and modern applications, hardware implementations based on FPGA for hash functions are prone to errors due to noise and radiation since a change in the state of a bit can trigger a completely different hash output than the expected one, due to the avalanche effect or diffusion, meaning that modifying a single bit changes most of the desired bits of the hash; thus, it is vital to detect and correct any error during the algorithm execution. Current hardware solutions mainly seek to detect errors but not correct them (e.g., using parity checking or scrambling). To the best of our knowledge, there are no solutions that detect and correct errors for SHA-3 hardware implementations. This article presents the design and a comparative analysis of four FPGA architectures: two without fault tolerance and two with fault tolerance, which employ Hamming Codes to detect and correct faults for SHA-3 using an Encoder and a Decoder at the step-mapping functions level. Results show that the two hardware architectures with fault tolerance can detect up to a maximum of 120 and 240 errors, respectively, for every run of KECCAK-p, which is considered the worst case. Additionally, the paper provides a comparative analysis of these architectures with other works in the literature in terms of experimental results such as frequency, resources, throughput, and efficiency.