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The mangrove red snapper, Lutjanus argentimaculatus, is a marine food fish of economic and aquaculture importance. The application of genomic selection-based breeding programs for this species is limited by the absence of a reference genome and transcriptome profiles. The current study attempted to fill this void by generating genomic and transcriptomic resources for red snapper. Using PacBio long reads, and Arima Hi-C linked reads, a scaffold-level genome assembly was generated for L. argentimaculatus. The assembly is of 1.03 Gb comprising of 400 scaffolds with N50 of 33.8 Mb and was assessed to be 97.2% complete upon benchmarking with BUSCO. Full-length transcriptome generated with PacBio Iso-Sequencing strategy using six tissues (muscle, gills, liver, kidney, stomach, and gonad) contained 56,515 isoforms belonging to 18,108 unique genes with N50 length of 3,973 bp. The resources generated will have potential applications in the functional studies, conservation, broodstock management and selective breeding programmes of L. argentimaculatus. [ABSTRACT FROM AUTHOR]

Flow cytometry analysis of haemocytes from control and WSSV-infected shrimps (Group I) revealed that maximum cells were in the G1 phase of the cell cycle as only un-replicated cells are found in this phase (Figure 1a). Many viruses are known to interfere and block the cell cycle (Davy & Doorbar, 2007) or promote cell cycle progression as this would benefit the virus to use the host machinery for its own replication. Other studies also showed a significant increase in ROS during the early infection stages of WSSV with the highest value (2.7 times as compared with the control group) appearing at 3 h after WSSV injection and the high levels were maintained till 48 h post WSSV infection (Ji et al., 2011). Keywords: cell cycle; cytoplasmic free calcium; flow cytometry; Penaeus vannamei; respiratory burst; white spot syndrome virus EN cell cycle cytoplasmic free calcium flow cytometry Penaeus vannamei respiratory burst white spot syndrome virus 320 324 5 12/20/21 20220101 NES 220101 DNA viruses are known to interfere with host cell cycles, which helps in viral replication and adopt different strategies to act on cell cycle regulators for creation of a favourable cell environment (De Beeck & Caillet-Fauquet, 1997). [Extracted from the article]