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Objective. Potential usage of dry electrodes in emerging applications such as wearable devices, flexible tattoo circuits, and stretchable displays requires that, to become practical solutions, issues such as easy fabrication, strong durability, and low-cost materials must be addressed. The objective of this study was to propose soft and dry electrodes developed from polydimethylsiloxane (PDMS) and carbon nanotube (CNT) composites. Approach. The electrodes were connected with both conventional and in-house NTAmp biosignal instruments for comparative studies. The performances of the proposed dry electrodes were evaluated through electromyogram, electrocardiogram, and electroencephalogram measurements. Main results. Results demonstrated that the capability of the PDMS/CNT electrodes to receive biosignals was on par with that of commercial electrodes (adhesive and gold-cup electrodes). Depending on the type of stimuli, a signal-to-noise ratio of 5-10 dB range was achieved. Significance. The results of the study show that the performance of the proposed dry electrode is comparable to that of commercial electrodes, offering possibilities for diverse applications. These applications may include the physical examination of vital medical signs, the control of intelligent devices and robots, and the transmission of signals through flexible materials., (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)

In order to implement the fifth generation (5G) communication system for a large number of users, the governments of many countries nominated the low 5G frequency band between 3.3 and 4.3 GHz. This paper proposes a wideband RFPA by designing the input matching network (MN) and output MN of the device using the simplified real frequency technique (SRFT) and the harmonic tuning network. The load-pull and source-pull is applied at multiple points for 100 MHz intervals over the bandwidth to obtain the optimum impedances at the output and input of the 10W Gallium Nitride (GaN) Cree CGH40010F device. To verify the design, the RFPA is simulated, and the performance is measured between 3.3 and 4.3 GHz. According to experimental findings, the measured drain efficiency (DE) throughout the whole bandwidth ranged from 57.5 to 67.5% at the output power of 40 dBm. Moreover, at the 1 dB compression point between 39.2 and 42.2 dBm output power, the drain efficiency (DE) achieves a high value of 81.2% with an output power of 42.2 dBm at a frequency of 3.3 GHz. The RFPA can obtain a maximum gain of 12.4 dB at 3.5 GHz. The linearity of the RFPA with a two-tone signal is measured and the value is less than -22 dBc all over the band., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Sadeque et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Purpose: The drift in radiofrequency (RF) power amplifiers (RFPAs) is assessed and several contributing factors are investigated. Two approaches for prospective correction of drift are proposed and their effectiveness is evaluated., Methods: RFPA drift assessment encompasses both intra-pulse and inter-pulse drift analyses. Scan protocols with varying flip angle (FA), RF length, and pulse repetition time (TR) are used to gauge the influence of these parameters on drift. Directional couplers (DICOs) monitor the forward waveforms of the RFPA outputs. DICOs data is stored for evaluation, allowing calculation of correction factors to adjust RFPAs' transmit voltage. Two correction methods, predictive and run-time, are employed: predictive correction necessitates a calibration scan, while run-time correction calculates factors during the ongoing scan., Results: RFPA drift is indeed influenced by the RF duty-cycle, and in the cases examined with a maximum duty-cycle of 66%, the potential drift is approximately 41% or 15%, depending on the specific RFPA revision. Notably, in low transmit voltage scenarios, FA has minimal impact on RFPA drift. The application of predictive and run-time drift correction techniques effectively reduces the average drift from 10.0% to less than 1%, resulting in enhanced MR signal stability., Conclusion: Utilizing DICO recordings and implementing a feedback mechanism enable the prospective correction of RFPA drift. Having a calibration scan, predictive correction can be utilized with fewer complexity; for enhanced performance, a run-time approach can be employed., (© 2024 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals LLC on behalf of International Society for Magnetic Resonance in Medicine.)

Purpose: This project addressed the uses of a loudspeaker array for audiometric measurements. It sought to evaluate a prototype compact array in terms of the reliability of test results across sound booths., Method: A seven-loudspeaker array was developed to deliver sounds from -60° to +60° on an arc with a radius of 0.5 m. The system was equipped with a head position sensing system to maintain the listener's head near the optimal test position. Three array systems were distributed to each of the two test sites for within-subject assessments of booth equivalence on tests of sound localization, speech reception in noise, and threshold detection. A total of 36 subjects participated, 18 at each test site., Results: Results showed excellent interbooth consistency on tests of sound localization using speech and noise signals, including conditions in which one or both ears were covered with a muff. Booth consistency was also excellent on sound field threshold measurements for detecting quasi-diffuse noise bands. Nonequivalence was observed in some cases of speech-in-noise tests, particularly with a small one-person booth. Acoustic analyses of in situ loudspeaker responses indicated that some of the nonequivalent comparisons on speech-in-noise tests could be traced to the effects of reflections., Conclusions: Overall, the results demonstrate the utility and reliability of a compact array for the assessment of localization ability, speech reception in noise, and sound field thresholds. However, the results indicate that researchers and clinicians should be aware of the reflection effects that can influence the results of sound field tests in which signal and noise levels from separate loudspeakers are critical.

A CMOS analog front-end (AFE) local-field potential (LFP) chopper amplifier with stimulation artifact tolerance, improved right-leg driven (RLD) circuit, and improved auxiliary path is proposed. In the proposed CMOS AFE LFP chopper amplifier, common-mode artifact voltage (CMAV) and differential-mode artifact voltage (DMAV) removal using the analog template removal method are proposed to achieve good signal linearity during stimulation. An improved auxiliary path is employed to boost the input impedance and allow the negative stimulation artifact voltage passing through. The common-mode noise is suppressed by the improved RLD circuit. The chip is implemented in 0.18- μm CMOS technology and the total chip area is 5.46-mm 2 . With the improved auxiliary path, the measured input impedance is larger than 133 M[Formula: see text] in the signal bandwidth and reaches 8.2 G[Formula: see text] at DC. With the improved RLD circuit, the measured CMRR is 131 - 144 dB in the signal bandwidth. Under 60-μs pulse width and 130-Hz constant current stimulation (CCS) with ±1-V CMAV and ±50-mV DMAV, the measured THD at the SC Amp output of fabricated AFE LFP chopper amplifier is 1.28%. The measurement results of In vitro agar tests have shown that with ±1.6-mA CCS pulses injecting to agar, the measured THD is 1.69%. Experimental results of both electrical and agar tests have verified that the proposed AFE LFP chopper amplifier has good stimulation artifact tolerance. The proposed CMOS AFE LFP chopper amplifier with analog template removal method is suitable for real-time closed-loop deep drain stimulation (DBS) SoC applications.

Photoacoustic imaging (PAI) utilizes the photoacoustic effect to record both vascular and functional characteristics of a biological tissue. Photoacoustic signals have typically low amplitude that cannot be read efficiently by data acquisition systems. This necessitates the use of one or more amplifiers. These amplifiers are somewhat bulky (e.g., the ZFL-500LN+, Mini-Circuits, USA, or 351A-3-50-NI, Analog Modules Inc., USA). Here, we describe the fabrication and development process of a transducer with a built-in low-noise preamplifier that is encased within the transducer housing. This new, to the best of our knowledge, design could be advantageous for applications where a compact transducer + preamplifier is required. We demonstrate the performance of this compact detection unit in a laser scanning photoacoustic microscopy system by imaging a rat ear ex vivo and a rat brain vasculature in vivo.

This paper presents a groundbreaking Ku-band 20W RF front-end power amplifier (PA), designed to address numerous challenges encountered by satellite communication systems, including those pertaining to stability, linearity, cost, and size. The manuscript commences with an exhaustive discussion of system design and operational principles, emphasizing the intricacies of low-noise amplification, and incorporating key considerations such as noise factors, stability analysis, gain, and gain flatness. Subsequently, an in-depth study is conducted on various components of the RF chain, including the pre-amplification module, driver-amplification module, and final-stage amplification module. The holistic design extends to the inclusion of the display and control unit, featuring the power-control module, monitoring module, and overall layout design of the PA. It is meticulously tailored to meet the specific demands of satellite communication. Following this, a thorough exploration of electromagnetic simulation and measurement results ensues, providing validation for the precision and reliability of the proposed design. Finally, the feasibility of that design is substantiated through systematic system design, prototype production, and exhaustive experimental testing. It is noteworthy that, in the space-simulation environmental test, emphasis is placed on the excellent performance of the Star Ku-band PA within the 13.75GHz to 14.5GHz frequency range. Detailed power scan measurements reveal a P1dB of 43dBm, maintaining output power flatness < ± 0.5dBm across the entire frequency and temperature spectrum. Third-order intermodulation scan measurements indicate a third-order intermodulation of ≤ -23dBc. Detailed results of power monitoring demonstrate a range from +18dBm to +54dBm. Scans of spurious suppression and harmonic suppression, meanwhile, show that the PA evinces spurious suppression ≤ -65dBc and harmonic suppression ≤ -60dBc. Rigorous phase-scan measurements exhibit a phase-shift adjustment range of 0° to 360°, with a step of 5.625°, and a phase-shift accuracy of 0.5dB. Detailed data from gain-scan measurements show a gain-adjustment range of 0dB to 30dB, with a gain flatness of ± 0.5dB. Attenuation error is ≤ 1%. These test parameters perfectly align with the practical application requirements of the technical specifications. When compared to existing Ku-band PAs, our design reflects a deeper consideration of specific requirements in satellite communication, ensuring its outstanding performance and uniqueness. This PA features good stability, high linearity, low cost, and compact modularity, ensuring continuous and stable power output. These features position the proposed system as a leader within the market. Successful orbital deployment not only validates its operational stability; it also makes a significant contribution to the advancement of China's satellite PA technology, generating positive socio-economic benefits., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Chen et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

Advances in brain-machine interfaces and wearable biomedical sensors for healthcare and human-computer interactions call for precision electrophysiology to resolve a variety of biopotential signals across the body that cover a wide range of frequencies, from the mHz-range electrogastrogram (EGG) to the kHz-range electroneurogram (ENG). Existing integrated wearable solutions for minimally invasive biopotential recordings are limited in detection range and accuracy due to trade-offs in bandwidth, noise, input impedance, and power consumption. This article presents a 16-channel wide-band ultra-low-noise neural recording system-on-chip (SoC) fabricated in 65nm CMOS for chronic use in mobile healthcare settings that spans a bandwidth of 0.001 Hz to 1 kHz through a featured sample-level duty-cycling (SLDC) mode. Each recording channel is implemented by a delta-sigma analog-to-digital converter (ADC) achieving 1.0 μ V rms input-referred noise over 1Hz-1kHz bandwidth with a Noise Efficiency Factor (NEF) of 2.93 in continuous operation mode. In SLDC mode, the power supply is duty-cycled while maintaining consistently low input-referred noise levels at ultra-low frequencies (1.1 μV rms over 0.001Hz-1Hz) and 435 M Ω input impedance. The functionalities of the proposed SoC are validated with two human electrophysiology applications: recording low-amplitude electroencephalogram (EEG) through electrodes fixated on the forehead to monitor brain waves, and ultra-slow-wave electrogastrogram (EGG) through electrodes fixated on the abdomen to monitor digestion.

A second-order voltage-controlled oscillator (VCO)-based continuous-time sigma-delta modulator (CTSDM) for current-sensing readout applications is proposed. Current signals from the sensor can directly be quantized by the proposed VCO-based CTSDM, which does not require any extra trans-impedance amplifiers. With the proportional-integral (PI) structure and a VCO phase integrator, the capability of second-order noise shaping is available to reduce the in-band quantization noise. The PI structure can be simply realized by a resistor in series with the integrating capacitor, which can reduce the architecture complexity and maintain the stability of the system. The current-steering digital-to-analog converter with tail and sink current sources is used on the feedback path for the subtraction of the current-type input signal. All the components of the circuit are scaling friendly and applicable to current-sensing readout applications in the Internet of Things (IoT). The proposed VCO-based CTSDM implemented in a 0.18-μm standard CMOS process has a measured signal-to-noise and distortion ratio (SNDR) of 74.6 dB at 10 kHz bandwidth and consumes 44.8 μw only under a supply voltage of 1.2 V, which can achieve a Figure-of-Merit (FoM) of 160.76 dB.

This article presents a local field potential (LFP)/action potential (AP) mode reconfigurable analog front-end (AFE) dedicated for the closed-loop vagus nerve stimulator (VNS). It combines an inverse electrical model of the intracranial electroencephalogram (iEEG) conducting in the brain tissues and been recorded at scalp as the extended electroencephalogram (EEEG). The AFE contains a LFP/AP mode reconfigurable EEEG preamplifier, a tunable integrator to compensate the effect of either the recording electrodes or head tissues, and an adder. The LFP/AP mode reconfigurable EEEG preamplifier consists of a tunable chopper-stabilized amplifier (CSA) and a 2nd-order tunable low pass filter (LPF). For better separation of LFP and AP signals, a high-order DC servo loop (DSL) characterized as a 2nd-order DSL in parallel with a 1st-order DSL is exploited in the tunable CSA to achieve a tunable high-pass frequency with a stopband attenuation slope (SAS) of +40 dB/dec. In addition, the tunable LPF can obtain a tunable low-pass frequency with a SAS of -40 dB/dec and provide additional 20 dB gain for AP signals. Fabricated in a SMIC 180 nm CMOS technology, and in the LFP band (0.5 Hz-200 Hz) and AP band (300 Hz-5 kHz), the measured mid-band gains of the LFP/AP mode reconfigurable EEEG preamplifier are 39.6 dB and 59.5 dB, the input-referred noises (IRNs) are 2.2 μVrms and 6.3 μVrms, the DC/in-band input impedances are 1.27/1.26 GΩ and 0.3/0.22 GΩ, respectively. The power consumption per channel AFE is 6.3 μW, and the die area is 1.4 mm × 0.25 mm.

MicroRNA detection is crucial for early infectious disease diagnosis and rapid cancer screening. However, conventional techniques like reverse transcription-quantitative polymerase chain reaction, requiring specialized training and intricate procedures, are less suitable for point-of-care analyses. To address this, we've developed a straightforward amplifier based on an exonuclease III (exo III)-propelled DNAzyme walker for sensitive and selective microRNA detection. This amplifier employs a specially designed hairpin probe with two exposed segments for strand recognition. Once the target microRNA is identified by the hairpin's extended single-strand DNA, exo III initiates its digestion, allowing microRNA regeneration and subsequent hairpin probe digestion cycles. This cyclical process produces a significant amount of DNAzyme, leading to a marked reduction in electrochemical signals. The biosensor exhibits a detection range from 10 fM to 100 pM and achieves a detection limit of 5 fM (3σ criterion). Importantly, by integrating an "And logic gate," our system gains the capacity for simultaneous diagnosis of multiple microRNAs, enhancing its applicability in RNA-based disease diagnostics., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)

This article presents the design of a low-power, low-noise neural signal amplifier for neural recording. The structure reduces the current consumption of the amplifier through current scaling technology and lowers the input-referred noise of the amplifier by combining a source degeneration resistor and current reuse technologies. The amplifier was fabricated using a 0.18 μm CMOS MS RF G process. The results show the front-end amplifier exhibits a measured mid-band gain of 40 dB/46 dB and a bandwidth ranging from 0.54 Hz to 6.1 kHz; the amplifier's input-referred noise was measured to be 3.1 μVrms, consuming a current of 3.8 μA at a supply voltage of 1.8 V, with a Noise Efficiency Factor (NEF) of 2.97. The single amplifier's active silicon area is 0.082 mm 2 .

This article describes a power-efficient, high dynamic range (DR) incremental ADC (IADC) for wearable biopotential signals recording, where DC and low-frequency disturbances such as electrode offset, 50/60 Hz interference and motion artifact must be tolerated. To achieve a wide DR, the IADC performs a three-step conversion by combining zoom-SAR and extended counting (EC) on top of a second-order incremental delta-sigma modulator (ΔΣM). The hybrid architecture notably reduces the oversampling ratio (OSR) with respect to conventional incremental ΔΣMs, while using the EC further improves the Signal-to-Noise-and-Distortion Ratio (SNDR) by 7.4 to 25.6 dB. Fabricated in a 0.18-μm CMOS technology, the IADC achieves 107.6-dB DR, 104.9-dB peak SNR, and 99.3-dB peak SNDR at 2 kS/s while dissipating 130 μW from 1.8-V (analog) / 1.2-V (digital) supply. This translates to a highly competitive FoM DR of 176.5 dB. The high-DR IADC reduces the gain of the preceding instrumentation amplifier (IA) such that significant DC and low-frequency disturbances can be tolerated. The advantages of high DR have been demonstrated by wearable Electrocardiography (ECG) and Electroencephalography (EEG) recordings under motion artifact.

We present the design, development, and experimental characterization of an active electrode (AE) IC for wearable ambulatory EEG recording. The proposed architecture features in-AE double common-mode (CM) rejection, making the recording's CMRR independent of typically-significant AE-to-AE gain variations. Thanks to being DC coupled and needless of chopper stabilization for flicker noise suppression, the architecture yields a super-T Ω input impedance. Such a large input impedance makes the AE's CMRR practically immune to electrode-skin interface impedance variations across different recording channels, a critical feature for dry-electrode ambulatory systems. Signal quantization and serialization are also performed in-AE, which enables a distributed system in which all AEs use a single data bus for data/command communication to the backend module, thus significantly improving the system's scalability. Additionally, the presented AE hosts auxiliary modules for (i) detection of an unstable electrode-skin connection through continuous interface impedance monitoring, (ii) dynamic measurement and adjustment of input DC level, and (iii) a CM feedback loop for further CMRR enhancement. The article also presents the development of printed (extrusion) tattoo electrodes and their experimental characterization results with the proposed AE architecture. Besides bio-compatibility, low-cost, pattern flexibility, and quick fabrication process, the printed electrodes offer a very stable electrode-skin connection, conform to scalp shape, and exhibit consistent performance under various bending curvatures. Analog circuit blocks of the presented AE architecture are designed and fabricated using a standard 180 nm CMOS technology, and the [Formula: see text] IC is integrated with off-chip low-power digital modules on a PCB to form the AE. Our measurement results show a CMRR of 82.2 dB (at 60 Hz), amplification voltage gain of 52.8 dB, a bandwidth of 0.2-400 Hz, ±500 mV input DC offset tolerance, An input impedance [Formula: see text], and 0.67 μV RMS integrated input referred noise (0.5-100 Hz), while consuming 17.5 μW per channel. All auxiliary modules are tested experimentally, and the entire system is validated in-vivo, for both ECG and EEG recording.

This article introduces a Combined .symmetrical and complementary Input Pairs (CIP) of a Differential Difference Amplifier (DDA), to boost the total Common-Mode Rejection Ratio (CMRR) for multi-channel neural signal recording. The proposed CIP-DDA employs three input pairs (transconductors). The dc-coupled input neural signal connection, via the gate terminal of the first transconductor, yields a high input impedance. The high-pass corner frequency and dc quiescent operation point are stabilized by the second transconductor. The calibration path of differential-mode gain and Common-Mode Feedback (CMFB) is provided by the proposed third transconductor. The parallel connection has no need for extra voltage headroom of input and output. The proposed CIP-DDA is targeted at integrated circuit realization and designed in a 0.18-μm CMOS technology. The proposed CIP-DDAs with system CMFB achieve an average CMRR of 103 dB, and each channel consumes circa 3.6 μW power consumption.

Filter Bank Multi-Carrier (FBMC) is attracting significant interest as a multi-carrier modulation (MCM) approach for future communication systems. It offers numerous advantages in contrast to Orthogonal Frequency Division Multiplexing (OFDM). Nonetheless, similar to many other MCM techniques, FBMC encounters a significant challenge with a high Peak-to-Average Power Ratio (PAPR). Additionally, incorporating Multiple-Input and Multiple-Output (MIMO) into FBMC presents heightened difficulties due to the presence of complex interference and increased computational complexity. In this paper, we first study the performance analysis of MIMO based Quadrature Amplitude Modulation (QAM)-FBMC systems considering the system complexity and interference. To enhance coverage effectively using beamforming with multiple antennas, it is essential to reduce PAPR to minimize the input backoff (IBO) required by nonlinear power amplifiers. Therefore, we propose new PAPR reduction method for MIMO based QAM-FBMC systems leveraging the null space within the MIMO channel using clipping and filtering (CF) technique. The PAPR reduction signals generated in this process are then mapped to the null space of the overall MIMO channel for each frequency block. Through computer simulations using a nonlinear power amplifier model, we illustrate that the proposed method substantially enhances both PAPR and throughput of MIMO based FBMC systems compared to conventional methods., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 Emad S. Hassan. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

The health and fitness of the human body rely heavily on physiological parameters. These parameters can be measured using various tools such as ECG, EMG, EEG, EOG, among others, to obtain real-time physiological data. Analysing the bio-signals obtained from these measurements can provide valuable information that can be used to improve health-care in terms of observation, diagnosis, and treatment. In bio-signal pattern recognition applications, more channels provide multiple information simultaneously. Different biosignal acquisition devices are available in the market, most of which are designed for specific signals like ECG, EMG, EEG etc The gain of the amplifiers and frequency of the filters are designed as per the targeted signals; due to which one device cannot be used for other signals. Also, most of the systems are wired system which is not comfortable for animal studies. In this paper, a low-cost, compact, wireless, 16 channel biopotential data acquisition system with integrated electrical stimulator is designed and implemented. There are several novel and flexible design approaches were incorporated in the proposed design like (1) It has user selectable digital filter in each channel based on the signal frequencies like ECG, EMG, EEG, EOG. The same system will be used to acquire different signals simultaneously. (2) It has variable gain with a configurable analog bandpass filter. (3) It can acquire signals from 4 patients simultaneously. (4) The system is capable to acquire signal from both two-electrode as well as three-electrode configurations. (5) It has integrated stimulator with trapezoidal, charge-balanced, biphasic stimulus output with near zero DC level and user selectable pulse duration or frequency of the stimulus. The developed system has the ability to acquire and transmit data wirelessly in real-time at a high transfer rate. To validate the performance of the system, tests were conducted on the acquired signals using a simulator., (© 2024 IOP Publishing Ltd.)

In the actual design process of traditional power amplifiers, there is a problem of being cumbersome and unable to simultaneously meet low power and saturation modes. Therefore, an improved multi-objective optimization algorithm proposed by decomposition is introduced to optimize its matching network to achieve overall optimization design of power amplifiers. The algorithm, matching network, and optimized power amplifier performance are simulated and verified. The experimental outcomes denote that on the logic function with Zener diode transistor, the proposed algorithm has a mean generation distance index of 5.03E-3, which is lower than most algorithms. Its overall comprehensive performance is better than the comparison algorithm, and compared to the comparison algorithm, it converges more quickly in the early stage of iteration on 1 and 2, and tends to stabilize in the 40th generation, and completes convergence in the 80th generation. In addition, the optimal solution has already begun to appear around the 25th generation and reached saturation around the 70th generation. At the same time, in the actual working bandwidth, the optimized power amplifier saturation efficiency reaches 51.5%~61.9%, and the efficiency at 6dB power backoff is about 44.4%~56.5%. Overall, the algorithm proposed in the study is effective in optimizing power amplifiers and their matching networks, effectively solving the problem of insufficient efficiency in low power modes in traditional designs., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2023 Jun Sun. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)

In this study, we explore nonlinear cochlear amplification by analyzing basilar membrane (BM) motion in the mouse apex. Through in vivo, postmortem, and mechanical suppression recordings, we estimate how the cochlear amplifier nonlinearly shapes the wavenumber of the BM traveling wave, specifically within a frequency range where the short-wave approximation holds. Our findings demonstrate that a straightforward mathematical model, depicting the cochlear amplifier as a wavenumber modifier with strength diminishing monotonically as BM displacement increases, effectively accounts for the various experimental observations. This empirically derived model is subsequently incorporated into a physics-based "overturned" framework of cochlear amplification [see Altoè, Dewey, Charaziak, Oghalai, and Shera (2022), J. Acoust. Soc. Am. 152, 2227-2239] and tested against additional experimental data. Our results demonstrate that the relationships established within the short-wave region remain valid over a much broader frequency range. Furthermore, the model, now exclusively calibrated to BM data, predicts the behavior of the opposing side of the cochlear partition, aligning well with recent experimental observations. The success in reproducing key features of the experimental data and the mathematical simplicity of the resulting model provide strong support for the "overturned" theory of cochlear amplification., (© 2023 Acoustical Society of America.)

Proteins are arguably one of the most important class of biomarkers for health diagnostic purposes. Label-free solid-state nanopore sensing is a versatile technique for sensing and analyzing biomolecules such as proteins at single-molecule level. While molecular-level information on size, shape, and charge of proteins can be assessed by nanopores, the identification of proteins with comparable sizes remains a challenge. Here, solid-state nanopore sensing is combined with machine learning to address this challenge. The translocations of four similarly sized proteins is assessed using amplifiers with bandwidths (BWs) of 100 kHz and 10 MHz, the highest bandwidth reported for protein sensing, using nanopores fabricated in <10 nm thick silicon nitride membranes. F-values of up to 65.9% and 83.2% (without clustering of the protein signals) are achieved with 100 kHz and 10 MHz BW measurements, respectively, for identification of the four proteins. The accuracy of protein identification is further enhanced by classifying the signals into different clusters based on signal attributes, with F-value and specificity of up to 88.7% and 96.4%, respectively, for combinations of four proteins. The combined use of high bandwidth instruments, advanced clustering and machine learning methods allows label-free identification of proteins with high accuracy., (© 2023 The Authors. Small Methods published by Wiley-VCH GmbH.)

This article presents a multichannel EEG/BIOZ acquisition application specific integrated circuit (ASIC) with 4 EEG channels and a BIOZ channel, a switch resistor low-pass filter (SR-LPF). Each EEG channel includes a frontend, and a 4-channel multiplexed analog-to-digital converter (ADC), while the BIOZ channel features a pseudo sine current generator and a pair of readout paths with multiplexed SR-LPF and ADC. The ASIC is designed for size and power minimization, utilizing a 3-step ADC with a novel signal-dependent low power strategy. The proposed ADC operates at a sampling rate of 1600 S/s with a resolution of 15.2 bits, occupying only 0.093 mm 2 . With the help of the proposed signal-dependent low-power strategy, the ADC's power dissipation drops from 32.2 μW to 26.4 μW, resulting in an 18% efficiency improvement without performance degradation. Moreover, the EEG channels deliver excellent noise performance with a NEF of 7.56 and 27.8 nV/√Hz at the expense of 0.16 mm 2 per channel. In BIOZ measurement, a 5-bit programmable current source is used to generate pseudo sine injection current ranging from 0 to 22 μApp, and the detection sensitivity reaches 2.4 mΩ/√Hz. Finally, the presented multichannel EEG/BIOZ acquisition ASIC has a compact active area of 1.5 mm 2 in an 180nm CMOS technology.

Body channel communication (BCC) has become a promising candidate in wireless body area networks (WBAN) due to its advantages in energy efficiency and security. However, BCC transceivers face two challenges: diverse application requirements and varying channel conditions. To overcome these challenges, this article proposes a re-configurable architecture for BCC transceivers (TRXs), whose key parameters and communication protocols can be software-defined (SD) according to the requirements. In the proposed TRX, the programmable direct-sampling receiver (RX) is a combination of a programmable low-noise amplifier (LNA) and a fast-convergent successive approaching register analog-to-digital converter (SAR ADC), to achieve simple but energy-efficient data reception. The programmable digital transmitter (TX) is essentially implemented by a 2-bit DAC array to transmit either wide-band carrier-free signals like 4-level pulse amplitude modulation (PAM-4) or non-return-to-zero (NRZ) or narrow-band carrier-based signals like on-off keying (OOK) or frequency shift keying (FSK). The proposed BCC TRX is fabricated in a 180-nm CMOS process. Through an in-vivo experiment, it achieves up to 10-Mbps data rate and 119.2 pJ/bit energy efficiency. Moreover, the TRX is able to communicate under long-distance (1.5 m) and body-shielding conditions by switching its protocols, which shows the potential to be deployed in all categories of WBAN applications.

This article presents a chip designed for wireless intra-cardiac monitoring systems. The design consists of a three-channel analog front-end, a pulse-width modulator featuring output-frequency offset and temperature calibration, and inductive data telemetry. By employing a resistance boosting technique in the instrumentation amplifier feedback, the pseudo-resistor exhibits lower non-linearity, leading to a total harmonic distortion of below 0.1%. Furthermore, the boosting technique enhances the feedback resistance, leading to a reduction in the size of the feedback capacitor and, consequently, the overall size. To make the modulator's output frequency resilient to temperature and process changes, coarse and fine-tuning algorithms are used. The front-end channel is capable of extracting the intra-cardiac signal with an effective number of bits of 8.9, while exhibiting an input-referred noise of less than 2.7 μV rms , and consuming 200 nW per channel. The front-end output is encoded by an ASK-PWM modulator, which drives an on-chip transmitter at 13.56 MHz. The proposed System-on-Chip (SoC) is fabricated in a 0.18 μm standard CMOS technology and consumes 4.5 μW while occupying 1.125 mm 2 .

This article presents a low-noise high-power-efficiency analog front-end (AFE) for capacitive-micromachined-ultrasonic transducers (CMUT). Implemented in 28-nm CMOS technology, the proposed AFE features three-stage continuous time-gain compensation (TGC) embedded in both trans-impedance amplifiers (TIAs) and an analog beamformer to provide a large compensation range with no extra power-consumption cost. The use of noise cancellation and capacitive feedback optimizes the noise performance of TIAs. The first stage of the TGC is built in the TIA by adjusting the positive and negative resistance loads, which are composed of voltage-controlled transistor arrays. An all-pass passive network is used as the delay unit of the analog beamformer, meanwhile achieving the second TGC stage. Phase shift for all frequency components in the ultrasound pass-band is manifested as a delay to the echoes. The third stage of the TGC is merged with a summing unit, which is a closed-loop amplifier with variable resistance feedback. The design takes into account the ability to handle large signals and power consumption, with TIA and beamforming operating at voltages of 2.5 V and 0.9 V, respectively. Experimental results show that the proposed AFE achieves a 2.11 pA/√Hz input-referred noise (IRN) at the 5 MHz center frequency of the echoes while consuming only 1.02 mW/channel. A total exponential TGC range of 60 dB with continuous ranges of 12 dB, 24 dB, and 24 dB assigned to three stages has been verified for this work.

Objective. Invasive brain-computer interfaces (BCIs) have shown promise in restoring motor function to those paralyzed by neurological injuries. These systems also have the ability to restore sensation via cortical electrostimulation. Cortical stimulation produces strong artifacts that can obscure neural signals or saturate recording amplifiers. While front-end hardware techniques can alleviate this problem, residual artifacts generally persist and must be suppressed by back-end methods. Approach. We have developed a technique based on pre-whitening and null projection (PWNP) and tested its ability to suppress stimulation artifacts in electroencephalogram (EEG), electrocorticogram (ECoG) and microelectrode array (MEA) signals from five human subjects. Main results. In EEG signals contaminated by narrow-band stimulation artifacts, the PWNP method achieved average artifact suppression between 32 and 34 dB, as measured by an increase in signal-to-interference ratio. In ECoG and MEA signals contaminated by broadband stimulation artifacts, our method suppressed artifacts by 78%-80% and 85%, respectively, as measured by a reduction in interference index. When compared to independent component analysis, which is considered the state-of-the-art technique for artifact suppression, our method achieved superior results, while being significantly easier to implement. Significance. PWNP can potentially act as an efficient method of artifact suppression to enable simultaneous stimulation and recording in bi-directional BCIs to biomimetically restore motor function., (© 2023 IOP Publishing Ltd.)

This article presents a prototype of a new, non-invasive, cuffless, self-calibrating blood pressure measuring device equipped with a pneumatic pressure sensor. The developed sensor has a double function: it measures the waveform of blood pressure and calibrates the device. The device was used to conduct proof-of-concept measurements on 10 volunteers. The main novelty of the device is the pneumatic pressure sensor, which works on the principle of a pneumatic nozzle flapper amplifier with negative feedback. The developed device does not require a cuff and can be used on arteries where cuff placement would be impossible (e.g., on the carotid artery). The obtained results showed that the systolic and diastolic pressure measurement errors of the proposed device did not exceed ±6.6% and ±8.1%, respectively.

Herein, we synthesized a novel porphyrinic covalent organic polymer (TPAPP-PTCA PCOP) for constructing a polarity-switchable dual-wavelength photoelectrochemical (PEC) biosensor with ferrocene (Fc) and hydrogen peroxide (H 2 O 2 ) as regulator and amplifier simultaneously. Interestingly, this new PCOP possessed both n-type and p-type semiconductor characteristics, which thus enabled the appearance of a dual-polarity photocurrent at two different excitation wavelengths. Furthermore, Fc and H 2 O 2 could readily switch the photocurrent of PCOP to the cathode and anode stemming from its efficient electron collection and donation function, respectively. Based on these, a PCOP-based PEC biosensor skillfully integrating dual wavelengths with reliable accuracy and polarity switch with high sensitivity was instituted. As a result, the developed PEC biosensor exhibited a low detection limit down to 0.089 pg mL -1 for the most powerful natural carcinogen aflatoxin M1 (AFM1) assay. Impressively, the target exhibited a completely opposite photocurrent difference to the interfering substances, and the linear correlation coefficient of the assay was improved compared to single-wavelength detection. The PEC sensing platform not only provided a basis for exploring multicharacteristic photoactive material but also innovatively developed the detection mode of the PEC biosensor.

In this research, to improve the ability of nanomaterials to interfere with liquid crystal orientation, we constructed a novel liquid crystal biosensor using Fe 3 O 4 nanospheres as signal amplifiers, which was used for the highly selective and sensitive detection of human chorionic gonadotropin (HCG). The Fe 3 O 4 nanospheres in liquid crystal biosensors are still rare, in particular for the detection of the HCG antigen, a marker of pregnancy in women. This strategy takes advantage of the large spatial structure of Fe 3 O 4 nanospheres to pre-immobilise β-hCG antibodies on the glass substrate, and, in the presence of HCG antigens, the antigen-antibody formed a specific immune response to disrupt the orientation of the liquid crystal. This allows an amplified optical signal to be generated for the ultimate successful detection of HCG antigen, and can greatly reduce the detectability of the target antigen concentration, ultimately greatly increasing the sensitivity of detection to a large degree. Thus, under optimal detection conditions, the minimum detection limit can be as low as 0.438 mIU mL -1 . The prepared LC biosensor demonstrates excellent specificity and sensitivity compared to conventional nanomaterials used in liquid crystal biosensors, and will be of great benefit for future applications requiring trace detection of proteins, antibiotics, bacteria and so on, which is expected to provide a sensitive detection platform for HCG and other molecular monitors.

One of the main challenges of analyzing intact proteins on an ion trap mass spectrometer is the mass range limitation, especially for miniature mass spectrometers. In this study, a high-field frequency scanning ion trap miniature mass spectrometer, namely the high-field "Brick" mass spectrometer, was developed to analyze intact proteins. A high-voltage broadband radio frequency (rf) amplifier was designed with a maximum output of 900 V p-p over a frequency range of 130-700 kHz. Compared to the 600 V p-p rf amplifier equipped in the conventional "Brick" mass spectrometer, the mass range of the instrument could be extended from 2000 to over 8000 Th. Sensitivity and mass resolution for native protein analyses were also evaluated and compared. Various proteins as well as their mixtures were analyzed on the high-field "Brick" mass spectrometer. The noncovalent interaction between protein-ligand complexes, lysozyme with triN-acetylchitotriose, was also analyzed. In addition, a hybrid ion scan mode was explored to further expand the mass range of the instrument at both low- and high-mass ends. In the hybrid ion scan mode, both rf frequency and amplitude were tuned, and a mass range from 100 to 12,000 Th was realized. As a result, both small drugs and proteins could be analyzed in a single mass scan. As proof-of-concept demonstrations, a mixture of atenolol and bovine serum albuminand oligomers of transferrin were analyzed.

Escherichia coli ( E. coli ) was used for cancer therapy due to the tumor-targeting, catalytic, and surface-reducing properties. Effective diagnosis combined with treatment of cancer based on E. coli , however, was rarely demonstrated. In this study, E. coli was used to surface reduce HAuCl 4 and as a carrier to modify riboflavin (Rf) and luminol (E-Au@Rf@Lum). After targeted delivery to tumor, the E-Au@Rf@Lum probe could actively emit 425 nm blue-violet chemiluminescence (CL) to achieve cell imaging for cancer diagnosis. Furthermore, this light could in situ trigger the photosensitizer (Rf) through CL resonance energy transfer, which produces reactive oxygen species (ROS) for accurate photodynamic therapy. In return, the excessive ROS enhanced the blue-violet light which was further absorbed by Rf, and ROS production was cyclically amplified. Abundant ROS broke down the dense extracellular matrix network and penetrated deep into tumors. Besides, E. coli with excellent catalytic property could decompose H 2 O 2 to O 2 to relieve tumor hypoxia for a long time and enhance the photosensitized process of Rf. By self-illumination, effective penetration, and tumor hypoxia relief, this work opens a self-amplified therapy modality to tumor.

Early detection of foodborne bacteria is urgently needed to ensure food quality and to avoid the outbreak of foodborne bacterial diseases. Here, a kind of metal-organic framework (Zr-MOF) modified with Pt nanoparticles (Pt-PCN-224) was designed as a peroxidase-like signal amplifier for microfluidic biosensing of foodborne bacteria. Taking Escherichia coli ( E. coli ) O157:H7 as a model, a linear range from 2.93 × 10 2 to 2.93 × 10 8 CFU/mL and a limit of detection of 2 CFU/mL were obtained. The whole detection procedure was integrated into a single microfluidic chip. Water, milk, and cabbage samples were successfully detected, showing consistency with the results of the standard culture method. Recoveries were in the range from 90 to 110% in spiked testing. The proposed microfluidic biosensor realized the specific and sensitive detection of E. coli O157:H7 within 1 h, implying broad prospects of MOF with biomimetic enzyme activities for biosensing.

Objective. Recent SiPM developments and improved front-end electronics have opened new doors in TOF-PET with a focus on prompt photon detection. For instance, the relatively high Cherenkov yield of bismuth-germanate (BGO) upon 511 keV gamma interaction has triggered a lot of interest, especially for its use in total body positron emission tomography (PET) scanners due to the crystal's relatively low material and production costs. However, the electronic readout and timing optimization of the SiPMs still poses many questions. Lab experiments have shown the prospect of Cherenkov detection, with coincidence time resolutions (CTRs) of 200 ps FWHM achieved with small pixels, but lack system integration due to an unacceptable high power uptake of the used amplifiers. Approach. Following recent studies the most practical circuits with lower power uptake (<30 mW) have been implemented and the CTR performance with BGO of newly developed SiPMs from Fondazione Bruno Kessler tested. These novel SiPMs are optimized for highest single photon time resolution (SPTR). Main results. We achieved a best CTR FWHM of 123 ps for 2 × 2 × 3 mm 3 and 243 ps for 3 × 3 × 20 mm 3 BGO crystals. We further show that with these devices a CTR of 106 ps is possible using commercially available 3 × 3 × 20 mm 3 LYSO:Ce,Mg crystals. To give an insight in the timing properties of these SiPMs, we measured the SPTR with black coated PbF 2 of 2 × 2 × 3 mm 3 size. We confirmed an SPTR of 68 ps FWHM published in literature for standard devices and show that the optimized SiPMs can improve this value to 42 ps. Pushing the SiPM bias and using 1 × 1 mm 2 area devices we measured an SPTR of 28 ps FWHM. Significance. We have shown that advancements in readout electronics and SiPMs can lead to improved CTR with Cherenkov emitting crystals. Enabling time-of-flight with BGO will trigger a high interest for its use in low-cost and total-body PET scanners. Furthermore, owing to the prompt nature of Cherenkov emission, future CTR improvements are conceivable, for which a low-power electronic implementation is indispensable. In an extended discussion we will give a roadmap to best timing with prompt photons., (Creative Commons Attribution license.)

In this article, we present a portable NMR relaxometry system optimized for the point-of-care analysis of body liquids such as blood. The presented system is centered on an NMR-on-a-chip transceiver ASIC, a reference frequency generator with arbitrary phase control, and a custom-designed miniaturized NMR magnet with a field strength of 0.29 T and a total weight of 330 g. The NMR-ASIC co-integrates a low-IF receiver, a power amplifier, and a PLL-based frequency synthesizer on a total chip area of 1100 × 900 μm 2 . The arbitrary reference frequency generator enables the use of conventional CPMG and inversion sequences, as well as modified water-suppression sequences. Moreover, it is used to implement an automatic frequency lock to correct temperature-induced magnetic field drifts. Proof-of-concept measurements on NMR phantoms and human blood samples show an excellent concentration sensitivity of v[Formula: see text] = 2.2 mM/[Formula: see text]. This very good performance renders the presented system an ideal candidate for the future NMR-based point-of-care detection of biomarkers such as the blood glucose concentration.

The key challenges in designing a multi-channel biosignal acquisition system for an ambulatory or invasive medical application with a high channel count are reducing the power consumption, area consumption and the outgoing wire count. This article proposes a spread-spectrum modulated biosignal acquisition system using a shared amplifier and an analog-to-digital converter (ADC). We propose a design method to optimize a recording system for a given application based on the required SNR performance, number of inputs, and area. The proposed method is tested and validated on real pre-recorded atrial electrograms and achieves an average percentage root-mean-square difference (PRD) performance of 2.65% and 3.02% for sinus rhythm (SR) and atrial fibrillation (AF), respectively by using pseudo-random binary-sequence (PRBS) codes with a code-length of 511, for 16 inputs. We implement a 4-input spread-spectrum analog front-end in a 0.18 μm CMOS process to demonstrate the proposed approach. The analog front-end consists of a shared amplifier, a 2nd order Σ∆ ADC sampled at 7.8 MHz, used for digitization, and an on-chip 7-bit PRBS generator. It achieves a number-of-inputs to outgoing-wire ratio of 4:1 while consuming 23 μA/input including biasing from a 1.8 V power supply and 0.067 mm 2 in area.