1. The droplet interface bilayer as a platform for a compartmentalized microreactor
- Author
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Allen-Benton, Maxwell and Booth, Paula Jane
- Subjects
572 - Abstract
Compartmentalization is one of the defining features of biological systems. Compartmentalization in biology can be defined as the use of physical barriers to spatially organise metabolic processes and create distinct microenvironments optimised for specific chemical reactions. Cells exhibit compartmentalization across all length scales, from the molecular scale to the cell membrane itself, which divides the cell from its environment. Compartmentalization serves to enhance the efficiency of metabolic processes by concentrating reaction intermediaries, localising key enzymes and excluding toxic by-products. Due to the positive effects that compartmentalization can impart on the efficiency of reactions, there is a desire to create synthetic compartmentalized systems in vitro. One technology which shows promise as a platform for constructing synthetic compartmentalized systems is the droplet interface bilayer. Droplet interface bilayers consist of two or more aqueous droplets coated with a lipid monolayer in an oil bath. Upon bringing the droplets into contact, a lipid bilayer is formed at the interface between the droplets. Membrane transport proteins can be functionally reconstituted into droplet interface bilayers, providing a mechanism for moving substrates from one droplet to another. This in turn leads to the possibility of conducting a cascade reaction across a series of droplets separated by droplet interface bilayers, with membrane protein transporters moving substrates and intermediaries throughout the network. This thesis presents a design for a droplet interface bilayer based compartmentalized microreactor.
- Published
- 2021