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Objective To investigate the clinical efficacy and safety of long-term application of omalizumab in the treatment of severe hormone dependent allergic asthma. Methods Seven cases of severe hormone dependent allergic asthma treated with omalizumab for 3 years were collected from Department of Respiratory Medicine, Zhongshan Hospital, Fudan University. Results of oral corticosteroid (OCS) doses, acute attack frequency, asthma control test (ACT), asthma quality of life (AQLQ), forced vital capacity (FVC), forced expiratory volume in first second (FEV1) and percentage to predicted value (FEV1% pred), and forced expiratory volume in the first second over forced vital capacity (FEV1/FVC) before and after omalizumab treatment were compared in this study. Results After treatment of omalizumab for 3 years, the oral prednisone doses of the 7 cases decreased from 15.0 (15.0-22.5) mg/d to 2.5（0-10.0) mg/d (P<0.01), and acute attack frequency decreased from 6 (4-7) per year to 0（0-1) per year (P<0.01). The ACT scores of 7 patients with severe asthma increased from 18.0 (13.0-20.0) to 22.0 (18.0-24.0), and AQLQ scores increased from (4.5±1.4) to (5.1±1.1). After treatment of omalizumab for 3 years, FEV1 increased from baseline (2.2±1.0) L to (2.5±1.3) L, FEV1% pred increased from baseline 74.4%±29.2% to 77.5%±27.2%, FEV1/FVC (%) increased from baseline 68.4%±11.9% to 69.8%±16.3%. One case had swelling at the injection site, and the rest had no obvious adverse reactions during the long-term treatment with omalizumab. Conclusion Long-term application of omalizumab in the treatment of severe hormone dependent allergic asthma could effectively reduce oral prednisone doses and acute attack frequency, control asthma symptoms and improve pulmonary ventilation function of patients and also has good safety. [ABSTRACT FROM AUTHOR]

The spatiotemporal characteristics are investigated by EOF method and Morlet wavelet analysis based on the spring daily minimum temperature of 13 representative weather stations in Eastern Liaoning mountainous region during 1960-2014. The probability distributions of each station’s extreme minimum temperature sequence are also modeled by the following methods:Gumble-I distribution, Weibull distribution, and Normal distribution. The best model was selected to calculate the extreme minimum temperature at different recurrence periods and occurrence probability and spatial distribution. We find that (1) The spatial distribution of spring extreme minimum temperature in the Eastern Liaoning mountainous region was mainly decomposed into the entire domain EOF mode, the north-south EOF mode, and the east-west EOF mode; (2) There were periods of 5 years and 10 years in the entire domain for extreme minimum temperature; (3) The Weibull distribution was the most appropriate model for the patterns of spring extreme minimum temperature in Eastern Liaoning mountainous region. The insignificant differences between predicted and measured extreme minimum temperature in different recurrence periods indicated minior errors (Absolute error <1℃, R2=0.98); (4) The northern area and high-altitude area are vulnerable to spring low-temperature disasters. The probability and distribution of mild low-temperature disasters is the largest, the moderate follows, and the severe is the minimal. In conclusion, our results provide scientific reference for the prediction of extreme minimum temperature in this region, and the prevention of recurring low-temperature disaster after spring warm-up. [ABSTRACT FROM AUTHOR]

This paper tried to propose a method to solve the problem of sentiment classification by integrating word embedding and convolutional neural network (CNN). First of all, the method accomplished a training process with skip-gram model to generate word embedding of each word in the dataset. Then, it created a two-dimensional feature matrix which was the combination of word embedding of each word in a training sample as the input of CNN model. Each iteration process of training, entries of feature matrix would also update as part of model parameters. Secondly, this paper proposed a CNN structure which was mainly composed of three different sizes of convolution kernels so as to complete the automatic extraction process of a variety of local abstract features. Compared with traditional machine learning algorithms, the proposed word embedding and CNN based sentiment classification model has successfully improved classification accuracy by 5.04%. [ABSTRACT FROM AUTHOR]

Bacteria BMJHZ-01, a strain capable of degrading difenoconazole was isolated from sewage sludges at a pesticide factory district in Jiangsu province. The morphology, physiological and biochemical identification for this strain was studied. 16S rDNA sequence homology analysis and phylogenetic tree alignment system indicated it as the Pseudomonas nitroreducens. Based on the study of the influence factors of degradation of difenoconazole, the most suitable degradation conditions for BMJHZ-01 was obtained as: Incubator speed 200 r/min and culture temperature of 30 ℃, initial pH value of 7.0. High performance liquid chromatography were used to detect pesticide concentration and its degradation rate was calculated. Difenoconazole with concentration of 180 mg/L could degraded more than 90% by the bacteria BMJHZ-01 within five days. BMJHZ-01 could grow and propagate in the highest difenoconazole concentration of 180 mg/L. The research results imply that this strain may be ultilized in future environmental management of difenoconazole pollution. [ABSTRACT FROM AUTHOR]

To further explore the internal mechanism of organic and inorganic fertilizer application on the fertilizer reduction and efficiency of dryland winter wheat, a fissure zone design was set up according to a 7-year long-term positioning experiment. Five nitrogen levels（0, 75, 150, 225, 300 kg·hm-2 ）were set as the main treatments; a mixed and unapplied organic fertilizer（dosage of 30 t·hm-2 ）was used as a side treatment. The single fertilizer treatment was labeled N0, N75, N150, N225, N300, and the combined organic fertilizer treatment was labeled MN0, MN75, MN150, MN225, MN300. The underground（0~20 cm root system）and aboveground parts of the plant were collected during the tillering, jointing, flowering, filling, and mature stages, and the grain yield and its constituent factors, nitrogen uptake, and utilization were studied. The results showed that, with an increase in the nitrogen application rate, the number of effective panicles and grains per panicle increased first and then decreased, while the 1 000-grain weight decreased gradually. The effective number of panicles in the combined organic fertilizer treatment increased by 7.1% compared with the single application of chemical fertilizer. The MN150 treatment had the highest grain yield, which reached 6 311 kg·hm-2 . The absorption of nitrogen under the single fertilizer treatment was mainly through pre-anthesis nitrogen transport, while pre-anthesis nitrogen transport and post-anthesis nitrogen absorption played equally important roles in the accumulation of nitrogen under the combined fertilizer treatment. Compared with the single application of chemical fertilizer, the combined application of organic and inorganic fertilizers increased pre-anthesis nitrogen transshipment, and post-anthesis nitrogen accumulation, by 9.8% and 33.1%, respectively. Pre-anthesis nitrogen transport mainly occurred in the stems, and nitrogen accumulation reached the highest under the MN150 treatment. Nitrogen use efficiency and agricultural efficiency were significantly increased by organic fertilizer application. A decrease in the nitrogen application rate increased the nitrogen use efficiency, agricultural efficiency, and nitrogen harvest index. Nitrate reductase activity in leaves was significantly increased by nitrogen application, and the highest value was reached under the N225 and MN150 treatments. Nitrate reductase activity increased by 25.94%, 12.88%, 9.90%, and 12.35% during the tiller, jointing, flowering, and filling stages, respectively, under organic and inorganic fertilizer application. In summary, the combined application of organic and inorganic fertilizers at a nitrogen application rate of 150 kg · hm-2 could not only promote the absorption and utilization of nitrogen in winter wheat plants, but also ensured a stable and high yield of winter wheat in a dryland in northwest China. [ABSTRACT FROM AUTHOR]

Objective To quantify the degree of pulmonary cystic damage in CT and compare its correlation with pulmonary function tests（PFTs）and serum levels of vascular endothelial growth factor （VEGF-D），and to study the potential of quantitative CT to assess the degree of lung damage and decide treatment in lymphangioleiomyomatosis（LAM）. Methods Pulmonary cystic areas in CT images were identified by the artificial intelligence software.The volumetric percentage of cystic regions of the total lung volume was calculated to get a cyst score.The association between the 36 times of CT scan and PFTs were analyzed in 21 patients. The correlation of serum VEGF-D levels of 17 patients and their 24 times of CT scan were studied.A ROC curve was calculated to select the threshold at which the cyst scores could predict the lung impairment best. Results Cyst scores correlated significantly with percentage of predicted forced of expiratory volume in one second （FEV1%pred）（r=-0.72，P<0.001） and percentage of predicted diffusion capacity（DLCO/VA%pred）（r=-0.821，P<0.000 1），while its correlation with serum VEGF-D levels（r=-0.143，P=0.506）was not obvious. The Youden index is max（0.667）with a 94.44% sensitivity and a 72.22% specificity when cyst score is 15.80%. Conclusion Pulmonary cyst scores on CT is strongly correlated with FEV1%pred and DLCO/VA% pred. However，the association with serum levels of VEGF-D is not obvious. Cyst score>15.80% might indicate an abnormal lung function and patients are recommended a sirolimus treatment. [ABSTRACT FROM AUTHOR]

The major monomer and oligomer of proanthocyanidins from the petals, seeds, fruits or pericarps of 11 plants were detected, and the difference of proanthocyanidin contents were analyzed in a variety of plants in this study. The catechin(C), epicatechin(EC), gallic acid(GA), procyanidin B2 (PCB2), procyanidin B4(PCB4), procyanidin A2(PCA2) of the 11 plants were extracted by 85% ethanol solution under ultrasonic, purified by Oasis PRiME HLB solid phase extraction column and separated by high performance liquid chromatography(HPLC). The result showed that monomers or oligomers of proanthocyanidins could be detected in 11 plants under the selected chromatographic conditions, and the content was mainly proanthocyanidin oligomers(OPC). Among them, the content of proanthocyanidins in grape seeds was the highest and the types were the most. Six proanthocyanidin monomers and oligomers were all detected in grape seeds. Two monomers, C and GA, and three oligomers, PCB2, PCB4 and PCA2, were detected in rose petals and peanut seed coats, respectively. In blueberry, walnut kernel, lycium ruthenicum murray and purple cabbage, except one proanthocyanidin monomer was detected, three kinds of proanthocyanidin oligomers were detected in blueberry and walnut kernel, while only PCB2 and PCB4 were detected in lycium ruthenicum murray and purple cabbage. The pericarp of red potato and purple potato only had OPCs; and the types of proanthocyanidin monomers and oligomers in red pepper and pericarps of dragon fruit were few and the content was low. In addition, the seed and pericarp of grape, peanut kernel and its seed coat were different parts of the same plant, and the types and content of proanthocyanidins were different. The types and contents of proanthocyanidins in different plants were different. The types and contents of proanthocyanidins in different parts of the same plant were also different. The differences of distribution, composition and concentration of proanthocyanidins in different plants lay a foundation for the study of structure-activity relationship and its development and utilization. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of rhIL-11 and possible mechanism, which might help to find a new theoretical basis for prevention and control measures. Methods SD rats were received artificial feeding, hypoxia, stomach LPS treatment to copy NEC animal models; rhIL-11 group rats was injected 400 μg/kg rhIL-11 respectively at the end of the NEC building，12 h，36 h, At this point, NEC and the control group rats injected with a dose of saline substitution; The rats were killed at 12h，36h，72h，120h after the completion of rhIL-11 injections； Effects of rhIL-11 on the intestinal histopathological changes were observed by HE staining. Results The body weight of NEC rats decreased which had significant differences with the control group (P<0.05) ； After reception of rhIL-11, rhIL-11 group of clinical manifestations of NEC gradually reduced and the weight gain faster than the NEC group; but on the whole of the experiment, the body weight of rhIL-11 group rats was always lower than the control group at the same time points there was differences between the two groups (P< 0.05). Intestinal epithelial cells was degeneration and necrosis, the number of crypts reduced significantly, Inflammatory cell infiltrated, lamina propria and muscle layer separated in severe parts. There was seepage9 hyperemia and hemorrhage in intestinal wall. Compared with the control groups the height of villus and the number of ileal crypt significantly reduced in NEC 2.0—3.0 days (P<0. 05). Intestinal of rhIL-11 group injured lighter than the NEC group, and the quantity of epithelial cells and crypt was mucher, the height of villus gradually restored. The height of villus and the number of ileal crypt gradually restored, There was no difference between rhIL-11 group and the control group after 120 h (P>0.05). Conclusion The main pathological changes of intestinal epithelium were degeneration, apoptosis and necrosis, the number of crypts reduced. Intestinal damage of rhIL-11 group was lighter than NEC group. The mechanism of might be that rhIL-11 reduced the damage of epithelial cells and crypts and Promoted crypt stem proliferation. [ABSTRACT FROM AUTHOR]

Objective To analyze the consistency of pulmonary function parameters measured by portable and Jaeger spirometer and explore the clinical feasibility of the portable spirometer. Methods The pulmonary ventilation functions of 151 subjects were simultaneously measuerd by Jaeger spirometer and Medikro PRO portable spirometer. Eight parameters.i. e. .forced vital capacity (FVC), forced expiratory volume in one second (FEV, ), precentage of predicted forced expiratory volume in one second (FEV, J'cpred)

Objective To study the effect of mesenchymal stem cell derived exosomes (MSC-Exo) on the proliferation of breast cancer cells. Methods The human breast cancer cell line MDA-MB-231 (231-Fluc-GFP) was constructed by lentiviral transfection using a double-reporter gene (fluorescein-green fluorescent protein, fluc-GFP) vector based on bioluminescence imaging technology, so that the cells were monitored in vitro in real time. Cells were treated with MSCs-Exo (MSCs-Exo group) and PBS (control group) respectively. The morphological observation, proliferation, survival rate and results of real-time PCR in vitro cells were performed in two groups of cells. In vivo, a nude mouse breast cancer model was established, and the tumor formation under different treatment conditions was analyzed. Results The proliferation of breast cancer MDA-MB-231 cells was inhibited after treatment with mesenchymal stem cell-derived exosomes. There were significant differences in cell numbers on the 3rd day of treatment between the two groups (P＜0.05). The survival rate of breast cancer cells decreased after treatment (P＜0.01). The expression of STAT3 and its downstream genes C-MYC，BCLXL， NANOG，OCT4 and SOX2 decreased in exosome treatment group. In vivo, the growth of tumor cells treated with MSCs- Exo was inhibited. Conclusion Mesenchymal stem cell-derived exosomes inhibit breast cancer cell growth [ABSTRACT FROM AUTHOR]

Objective · To investigate the effect of Lycium barbarum polysaccharides on inflammatory cytokines in type 2 diabetes mellitus (T2DM) mice without myeloid differentiation factor 88 gene (MyD88-/-). Methods · Levels of interleukin 1β (IL-1β), IL-6, IL-8, transforming growth factor β1 (TGF-β1), and IL-10 in serum were assessedELISA in the MyD88-/-T2DM mice which had been administered with different doses of LBP (20, 40, and 80 mg/kg). Momacrophages Raw264.7 were stimulatedlipopolysaccharide (LPS) after treatment with different concentrations of LBP (25, 50, and 100 μg/mL). Then Western blotting was used to detect nuclear translocation level of nuclear factor κB (NF-κB) and protein s of inhibitor of NF-κB (IκB) and p-IκB. Results · Serum levels of IL-1β and TGF-β1 in MyD88-/-T2DM mice were down-regulatedLBP (P<0.05). Cell experiment proved that nuclear migration of NF-κB was dose-dependently inhibitedLBP, and the level of p-IκB was reducedhigh dose of LBP. Conclusion · LBP can reduce some proinflammatory cytokines in the MyD88-/-T2DM mice, which may be related with its inhibitive effect on the phosphorylation of IκB and nuclear migration of NF-κB in the macrophages. [ABSTRACT FROM AUTHOR]

Objective To observe surgical safety for patients whose pulmonary functions manifest were obstructive ventilatory dysfunction. Methods The data of totally 122 patients with obstructive ventilatory dysfunction in their perioperative period, such as hospital stays, hospital costs, treatment methods and postoperative pulmonary adverse events, had been collected and analyzed. The influnce of different airway medications and different grades of obstructive ventilatory dysfunction on the above date were compared. Results According to the result of regression analysis, airway management medication had a highly significant negative correlation with postoperative pulmonary adverse events (b=-0.807, P<0.001) and hospital stays (b=-1.906, P=0.003), and adverse events decreased significantly if the patients were medicated in their airways before and after surgery compared with neither before or after surgery (P=0.013). There was no significant difference in the 4 study endpoints (postoperative pulmonary adverse events, hospital stays, ICU stays, hospital costs) among different groups of obstructive ventilatory dysfunction (P=0.240, 0.755, 0.360, 0.320), and the patients with severe obstructive ventilatory dysfunction were medicated more actively. While different groups of surgical type had significant difference of the 4 study endpoints (P all <0.001). Conclusions Airway management medication is the most essential factor of surgical safety for patients with obstructive ventilatory dysfunction. Even though preoperative pulmonary function manifests as severe obstructive ventilatory dysfunction, patients can go through the operation safely by effective airway management. [ABSTRACT FROM AUTHOR]