1. Isolation lactic acid bacteria from human breast milk and study their susceptibility to antibiotics.
- Author
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Nhaer, Ahlam Gareeb and Isa, Jawad Kadhim
- Subjects
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LACTIC acid bacteria , *BREAST milk , *PROBIOTICS , *BREASTFEEDING , *ANTIBIOTICS , *INFANT nutrition , *CESAREAN section - Abstract
Human milk is the primary source of infant nutrition and the transmission of various beneficial microorganisms. The lactic acid bacteria (LAB) in breast milk maintains the stability of the gut microflora of infants. This study was undertaken to evaluate the potential probiotic of LAB strains isolated from breastfeeding women milk at Al-Zahra Teaching Hospital of Wasit Province in Iraq, 50 samples of human breast milk. Three of (LAB): Lactobacillus, Bifidobacterium, and Streptococcus were recognized. They were selected and identified through morphology, Gram staining, and 16S rRNA, as the standard reference strain, the separated strains were described for their characteristics of growth, production of H2O2, and safety properties (including hemolytic activity, antibiotic susceptibility). From 50 positive samples belonged to human milk among them 26(52%) Bifidobacterium spp., 50(100%) Streptococcus spp., and 45(90%) Lactobacillus spp. There was relationship between the percent of delivery case naturally and by caesarean section in the milk samples (p≤0.05). There is no relationship between the age of the mother and the bacteria count. There are significance differences (p≤0.005) between baby age and bacterial count. To evaluate the probiotic potential of antibiotic susceptibility by disc diffusion method. Antibiotic resistance among (50) isolates was detected to Ampicillin, Tetracycline, vancomycin, Gentamicin, Trimethoprim, Levofloxacin, Amikacin, and Ciprofloxacin the isolates showed resistance against multiple antibiotics isolated were resistant to Amikacin, Gentamicin, and Levofloxacin and intermediately resistant to Ciprofloxacin but susceptible to Ampicillin, Tetracycline, Trimethoprim, and vancomycin. Depending on these properties, human breast milk is demonstrated as a source of potential probiotic bacteria. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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