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The current study conducted to identify the bacterial isolates from burns and wound infections that are responsible for various skin complication in such conditions and determination the development of antimicrobial resistance among these bacterial isolates. This study is carried out Between September 2020 to December 2020, a total number of (103) burns and wound swabs were taken from different patients from different hospitals in Baghdad city, the specimens were collected from; burns (57) and wound (46) swabs, the specimens were included; (51) female and (52) Male, the age average between (2 - 70 years). The positive bacterial containing specimens are (93) swabs, most of them are mixed (82 specimens) with different types of bacterial species and only (11 specimens) are contained with one type of bacterial isolate, while the negative bacterial containing are (10) swabs that showed no aerobic bacterial growth.. Staphylococcus sp. is recorded the most prevalence organisms found in wounds and burns 75/163(46%) included 31(41.3%) and 44(58.7%) isolated from burns and wounds respectively. Whereas Enterobacteriaceae sp. revealed that 57/163 ( 35%), 25(44%) from wounds and 32(56%) from burns. Also Pseudomonas sp. 23/163( 14.1%), Acinetobacter sp. 5/163(3.1%), and Corynebacterium sp. 3/163(1.8%) were isolated from infected wound in a percentage (47.8%,60%,and 33.3%) and from burns (52.2%, 40% and 66.7%) respectively. Another experiment was performed to measure the antimicrobial susceptibility against several antimicrobial agents included; Methicillin, Caphalothin, Tobramycin, Azithromycin, Bacitracin, Novobiocin, Levofloxacin and Sulfamethoxazole + Trimethoprim. The results showed increasing the resistance percentages among different bacterial isolates, and developed multidrug resistant bacteria. [ABSTRACT FROM AUTHOR]

Objective(s): P. mirabilis is considered as extensive drug resistant pathogen in many studies as it can resist complex antibiotic regimes, such pathogen can be threat to public health, especially when it has the ability to produce biofilm. Therefore, biofilm production was characterized among XDR P. mirabilis local isolates in this research. Material and Methods: 100 P. mirabilis isolated from wound infections from patients admitted to Baghdad hospitals. They were identified using biochemical test and Vitek2 system. The MIC test for antibiotic sensitivity was done by Vitek 2 automated system. Biofilm production was identified phenotypically by twitching motility test, scanning electron microscope, and microtiter plate assay. Results: It could be revealed that 8/100 isolates were MDR, 90/100 isolates were XDR and pan drug resistance level was shown in only two isolates. 80% of isolates had motile ability through twitching assay, and scan electron microscope study revealed that 76% of XDR isolates could produce different stages of biofilm on coverslip placed in MacConkey broth. Microtiter plate assay revealed 81% of XDR isolates were biofilm producers. Conclusion: it could be concluded that extensive drug resistant P. mirabilis can produce biofilm hence resist several important antibiotics; making treatment of infection among wounded patients is such a challenge in many hospitals in Baghdad. [ABSTRACT FROM AUTHOR]

Clinical specimens from burns, wounds and UTI`s, were collected from four hospitals in Baghdad area, and local isolates of P. aeruginosa and S. aureus were obtained. Five monosubstituted metal cholates were prepared by reacting monoequivalents of sodium cholate with metal salts in two different solvent, viz. 95% ethanol or water. Ten monosubstituted metal cholates were obtained, and characterized by their physical and spectrophotometric properties. It is the first time to report systematic preparation, characterization and study of such monosubstituted metal cholate. The growth curves for P. aeruginosa and S. aureus in the presence of metal cholates, revealed that the time for logarithmic phase were reduced from 25-30 to 5-25 minutes for both bacteria, while the stationary phase from 90-100 to 40-60 minutes. The antibacterial activities of the prepared metal cholates against P. aeruginosa and S. aureus were determined by broth dilution method in the presence of metal cholates. It was found that in the presence of the metal cholates, the MBC values were found to be within the range 8.0×10-5 - 8.35×10-5 M. [ABSTRACT FROM AUTHOR]